Base de dados : MEDLINE
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[PMID]:29458480
[Au] Autor:Pal M; Kumari M; Kiran S; Salwan R; Mayilraj S; Chhibber S; Gulati A
[Ad] Endereço:1​CSIR-Institute of Himalayan Bioresource Technology, Palampur (HP)-176 061, India.
[Ti] Título:Chryseobacterium glaciei sp. nov., isolated from the surface of a glacier in the Indian trans-Himalayas.
[So] Source:Int J Syst Evol Microbiol;68(3):865-870, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel bacterial strain, IHBB 10212 , of the genus Chryseobacterium was isolated from a glacier near the Kunzum Pass located in the Lahaul-Spiti in the North-Western Himalayas of India. The cells were Gram-negative, aerobic, non-sporulating, single rods, lacked flagella, and formed yellow to orange pigmented colonies. The strain utilized maltose, trehalose, sucrose, gentibiose, glucose, mannose, fructose, mannitol, arabitol and salicin for growth. Flexirubin-type pigments were produced by strain IHBB 10212 . The 16S rRNA gene sequence analysis showed relatedness of strain IHBB 10212 to Chryseobacterium polytrichastri DSM 26899 (97.43 %), Chryseobacterium greenlandense CIP 110007 (97.29 %) and Chryseobacterium aquaticum KCTC 12483 (96.80 %). Iso-C15 : 0 and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) constituted the major cellular fatty acids. The polar lipids present were six unidentified aminolipids, one unidentified phospholipid and three unidentified lipids. MK-6 was identified as the major quinone. The DNA G+C content was 34.08  mol%. Digital DNA-DNA hybridization of strain IHBB 10212 with C. polytrichastri, C. greenlandense and C. aquaticum showed values far below the prescribed thresholds of 95 % for average nucleotide identity and 70 % for the Genome-to-Genome Distance Calculator for species delineation. Based on its differences from validly published Chryseobacterium species, strain IHBB 10212 is identified as a new species, for which the proposed name is Chryseobacterium glaciei sp. nov., with IHBB 10212 as the type strain (=MTCC 12457 =JCM 31156 =KACC 19170 ).
[Mh] Termos MeSH primário: Chryseobacterium/classificação
Camada de Gelo/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Chryseobacterium/genética
Chryseobacterium/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Índia
Hibridização de Ácido Nucleico
Fosfolipídeos/química
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 71ANL51TLA (menaquinone 6)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002600


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[PMID]:29256852
[Au] Autor:Divyasree B; Suresh G; Sasikala C; Ramana CV
[Ad] Endereço:1​Bacterial Discovery Laboratory, Center for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad, 500085, India.
[Ti] Título:Chryseobacterium salipaludis sp. nov., isolated at a wild ass sanctuary.
[So] Source:Int J Syst Evol Microbiol;68(2):542-546, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, rod-shaped, non-motile, aerobic bacterium was isolated from a sediment sample obtained from a wild ass sanctuary in Gujarat, India. The strain designated JC490 was oxidase- and catalase-positive. The 16S rRNA gene sequence analysis and sequence comparison data indicated that strain JC490 was a member of the genus Chryseobacterium and was closely related to Chryseobacterium jeonii AT1047 (96.4 %) and with other members of the genus Chryseobacterium (<96.3 %). The DNA G+C content of strain JC490 was 34 mol%. Strain JC490 had phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipids and five unidentified polar lipids. Menaquinone-6 was the only respiratory quinone found. Iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0 3-OH were the major fatty acids of strain JC490 . On the basis of physiological, genotypic, phylogenetic and chemotaxonomic analyses, it is concluded that strain JC490 constitutes a novel species of the genus Chryseobacterium, for which the name Chryseobacterium salipaludis sp. nov. is proposed. The type strain is JC490 (=KCTC 52835 =LMG 30048 ).
[Mh] Termos MeSH primário: Chryseobacterium/classificação
Equidae
Sedimentos Geológicos/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
Chryseobacterium/genética
Chryseobacterium/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Índia
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 71ANL51TLA (menaquinone 6)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002536


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[PMID]:28213021
[Au] Autor:Alothman M; Lusk KA; Silcock P; Bremer PJ
[Ad] Endereço:Department of Food Science, University of Otago, 276 Leith Walk, 9054 Dunedin, New Zealand. Electronic address: mohammad.alothman@otago.ac.nz.
[Ti] Título:Comparing PTR-MS profile of milk inoculated with pure or mixed cultures of spoilage bacteria.
[So] Source:Food Microbiol;64:155-163, 2017 Jun.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The volatile organic compounds (VOCs) associated with UHT milk (n=8) inoculated with either pure inoculums of Pseudomonas fluorescens (two strains tested) or Chryseobacterium sp., or with mixed cultures of 2 or all 3 of the bacterial strains, and held at 4.5 °C for up to 26 days was measured using proton transfer reaction - mass spectrometry (PTR-MS). The VOCs evolved included a range of carbonyl compounds, alcohols, esters, and acids and had significant qualitative and quantitative differences between the inoculums. Milks inoculated with paired (mixed) bacterial cultures attained patterns similar to the VOC composition of one of the pure inoculums, which could be attributed to the domination of these bacteria within the mixed inoculum. This study will help to characterize the spoilage of milk and provide important insights into understanding the factors that limit the shelf life of milk.
[Mh] Termos MeSH primário: Chryseobacterium/metabolismo
Contaminação de Alimentos
Leite/química
Leite/microbiologia
Pseudomonas fluorescens/metabolismo
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Animais
Armazenamento de Alimentos
Temperatura Alta
Espectrometria de Massas/métodos
Prótons
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protons); 0 (Volatile Organic Compounds)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE


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[PMID]:28211309
[Au] Autor:Zhao Y; Wang Y; Li DH; Deng Y; Yang H
[Ad] Endereço:1​Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan, PR China.
[Ti] Título:Chryseobacterium reticulitermitis sp. nov., isolated from the gut of Reticulitermes aculabialis.
[So] Source:Int J Syst Evol Microbiol;67(6):1698-1702, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, strain Ra1T, was isolated from the gut of a wood-feeding lower termite, Reticulitermes aculabialis. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was closely related to Chryseobacterium rigui JCM 18078T (96.7 % similarity). Growth was observed at 15-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum 0 %). The DNA G+C content of strain Ra1T was 39.9 mol%. Cells contained menaquinone MK-6 as the sole respiratory quinone and the major fatty acids were iso-C15 : 0, iso-C17 : 0, summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 9 (comprising C16 : 0 10-methyl and/or iso-C17 : 1ω9c). The predominant polyamine was sym-homospermidine. The cellular polar lipids consisted of one phosphatidylethanolamine, three unidentified aminolipids, one unidentified phospholipid and one unidentified lipid. Based on phenotypic, genotypic and phylogenetic studies, it is concluded that strain Ra1T represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium reticulitermitis sp. nov. is proposed. The type strain is Ra1T (=CCTCC AB 2015431T=KCTC 52230T).
[Mh] Termos MeSH primário: Chryseobacterium/classificação
Isópteros/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
China
Chryseobacterium/genética
Chryseobacterium/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espermidina/análogos & derivados
Espermidina/química
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0IJ25X1H4R (sym-homospermidine); 11032-49-8 (Vitamin K 2); 71ANL51TLA (menaquinone 6); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001848


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[PMID]:28199699
[Au] Autor:Verner-Jeffreys DW; Brazier T; Perez RY; Ryder D; Card RM; Welch TJ; Hoare R; Ngo T; McLaren N; Ellis R; Bartie KL; Feist SW; Rowe WMP; Adams A; Thompson KD
[Ad] Endereço:Cefas Weymouth laboratory, The Nothe, Barrack Road, Weymouth DT4 8UB, UK.
[Ti] Título:Detection of the florfenicol resistance gene floR in Chryseobacterium isolates from rainbow trout. Exception to the general rule?
[So] Source:FEMS Microbiol Ecol;93(4), 2017 Apr 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacteria from the family Flavobacteriaceae often show low susceptibility to antibiotics. With the exception of two Chryseobacterium spp. isolates that were positive for the florfenicol resistance gene floR, no clinical resistance genes were identified by microarray in 36 Flavobacteriaceae isolates from salmonid fish that could grow in ≥ 4 mg/L florfenicol. Whole genome sequence analysis of the floR positive isolates revealed the presence of a region that contained the antimicrobial resistance genes floR, a tet(X) tetracycline resistance gene, a streptothricin resistance gene and a chloramphenicol acetyltransferase gene. In silico analysis of 377 published genomes for Flavobacteriaceae isolates from a range of sources confirmed that well-characterised resistance gene cassettes were not widely distributed in bacteria from this group. Efflux pump-mediated decreased susceptibility to a range of antimicrobials was confirmed in both floR positive isolates using an efflux pump inhibitor (phenylalanine-arginine ß-naphthylamide) assay. The floR isolates possessed putative virulence factors, including production of siderophores and haemolysins, and were mildly pathogenic in rainbow trout. Results support the suggestion that, despite the detection of floR, susceptibility to antimicrobials in Flavobacteriaceae is mostly mediated via intrinsic mechanisms rather than the horizontally acquired resistance genes more normally associated with Gram-negative bacterial pathogens such as Enterobacteriaceae.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Chryseobacterium/efeitos dos fármacos
Chryseobacterium/genética
Oncorhynchus mykiss/microbiologia
Tianfenicol/análogos & derivados
[Mh] Termos MeSH secundário: Acetiltransferases/genética
Animais
Proteínas de Transporte/antagonistas & inibidores
Proteínas de Transporte/genética
Cloranfenicol O-Acetiltransferase/genética
Chryseobacterium/isolamento & purificação
Genoma Bacteriano/genética
Proteínas Hemolisinas/biossíntese
Seres Humanos
Testes de Sensibilidade Microbiana
Fenilalanina/análogos & derivados
Fenilalanina/farmacologia
Reação em Cadeia da Polimerase
Sideróforos/biossíntese
Resistência a Tetraciclina/genética
Tianfenicol/farmacologia
Fatores de Virulência/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Carrier Proteins); 0 (Hemolysin Proteins); 0 (Siderophores); 0 (Virulence Factors); 47E5O17Y3R (Phenylalanine); 740-57-8 (phenylalanine-beta-naphthylamide); 9J97307Y1H (florfenicol); EC 2.3.1.- (Acetyltransferases); EC 2.3.1.- (streptothricin acetyltransferase); EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix015


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[PMID]:27902299
[Au] Autor:Chaudhary DK; Kim J
[Ad] Endereço:Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
[Ti] Título:Chryseobacterium nepalense sp. nov., isolated from oil-contaminated soil.
[So] Source:Int J Syst Evol Microbiol;67(3):646-652, 2017 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Biratnagar, Morang, Nepal, a yellow-coloured, Gram-staining-negative, aerobic, non-motile, and rod-shaped bacterium, designated strain C-5-3T, was isolated. This strain was characterized taxonomically by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain C-5-3T belonged to the genus Chryseobacterium and was closely related to Chryseobacterium profundimaris DY46T (98.19 % sequence similarity), Chryseobacterium takakiae AG1-2T (98.15 % sequence similarity), Chryseobacterium taiwanense BCRC 17412T (98.14 % sequence similarity), Chryseobacterium camelliae THG C4-1T (97.73 % sequence similarity) and Chryseobacterium hispalense DSM 25574T (97.60 % sequence similarity). The predominant respiratory quinone was menaquinone-6, and phosphatidylethanolamine was the major polar lipid. The predominant fatty acids of strain C-5-3T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH and summed feature 9 (iso- C17 : 1ω9c and/or C16 : 0 10-methyl). The genomic DNA G+C content of this novel strain was 38.6 mol%. The DNA-DNA relatedness between strain C-5-3T and Chryseobacterium profundimaris JCM 19801T, C. takakiae DSM 26898T, C. taiwanense KACC 13400T, C. camelliae KACC 16985T and C. hispalense DSM 25574T was 53.3, 42.7, 47.3, 33.0 and 28.0 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain C-5-3T represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium nepalense sp. nov. is proposed. The type strain is C-5-3T (=KEMB 9005-411T=KACC 18907T=JCM 31469T).
[Mh] Termos MeSH primário: Chryseobacterium/classificação
Poluição por Petróleo
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Chryseobacterium/genética
Chryseobacterium/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Nepal
Hibridização de Ácido Nucleico
Fosfatidiletanolaminas/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylethanolamines); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 39382-08-6 (phosphatidylethanolamine); 71ANL51TLA (menaquinone 6)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001680


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[PMID]:27902240
[Au] Autor:Zhao Z; Tu YQ; Shen X; Han SB; Zhang CY; Sun C; Wu M
[Ad] Endereço:1​College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
[Ti] Título:Chryseobacterium lineare sp. nov., isolated from a limpid stream.
[So] Source:Int J Syst Evol Microbiol;67(4):800-805, 2017 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, aerobic, non-motile, rod-shaped, yellow-pigmented bacterial strain, XC0022T, isolated from freshwater of a limpid stream in Zhejiang, China, was studied using a polyphasic approach. The phylogenetic analysis based on 16S rRNA gene sequences clearly showed an allocation to the genus Chryseobacterium with the highest sequence similarities of 98.0 % to Chryseobacterium taeanense PHA3-4T, 97.2 % to Chryseobacterium taihuense THMBM1T, 97.1 % to Chryseobacterium rigui CJ16T and 97.1 % to Chryseobacteriumprofundimaris DY46T. 16S rRNA gene sequence similarities to all other species of the genus Chryseobacterium were below 97.0 % (92.3-96.8 %). DNA-DNA hybridization results showed that strain XC0022T was 55.3 %, 49.8 % and 31.1 % related to C. taeanense DSM 17071T, Chryseobacteriumtaichungense DSM 17453T and Chryseobacteriumgleum JCM 2410T, respectively. The quinone system was composed only of MK-6. Strain XC0022T possessed iso-C15 : 0, iso-C17 : 0 3-OH, C18 : 1ω9c and summed feature 3 (iso-C15 : 0 2-OH/C16 : 1ω7c) as the major fatty acids. The polar lipids profile consisted of one phosphatidylethanolamine, one unidentified glycolipid, four unidentified aminolipids and two unidentified lipids. The G+C content of the genomic DNA was 29.7 mol%. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain XC0022T (=KCTC 52364T=MCCC 1K02723T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium lineare sp. nov. is proposed.
[Mh] Termos MeSH primário: Chryseobacterium/classificação
Filogenia
Rios/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
Chryseobacterium/genética
Chryseobacterium/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Hibridização de Ácido Nucleico
Fosfatidiletanolaminas/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Phosphatidylethanolamines); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 39382-08-6 (phosphatidylethanolamine); 71ANL51TLA (menaquinone 6)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001629


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[PMID]:27879557
[Au] Autor:Radera S; Tripathi S; Agarwal J; Kumar M
[Ad] Endereço:From the *Department of Microbiology, and †Department of Pediatrics, King George's Medical University, Lucknow, Uttar Pradesh, India.
[Ti] Título:Chryseobacterium Indologenes-Associated Pneumonia in 2 Neonates.
[So] Source:Pediatr Infect Dis J;36(3):337-339, 2017 Mar.
[Is] ISSN:1532-0987
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chryseobacterium species are widely distributed in nature and can rarely cause human infection. Few cases reported in hospitalized patients are in immunocompromised hosts with indwelling devices and associated comorbidities. Chryseobacterium species are usually multidrug resistant. We describe 2 cases of Chryseobacterium indologenes-associated pneumonia in neonates and review the published infant cases.
[Mh] Termos MeSH primário: Chryseobacterium
Infecções por Flavobacteriaceae
Pneumonia Bacteriana
Pneumonia Associada à Ventilação Mecânica
[Mh] Termos MeSH secundário: Seres Humanos
Recém-Nascido
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE
[do] DOI:10.1097/INF.0000000000001426


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[PMID]:27876290
[Au] Autor:Chidambarampadmavathy K; Karthikeyan OP; Huerlimann R; Maes GE; Heimann K
[Ad] Endereço:College of Science and Engineering, James Cook University, Townsville 4811, Queensland, Australia; Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville 4811, Queensland, Australia.
[Ti] Título:Response of mixed methanotrophic consortia to different methane to oxygen ratios.
[So] Source:Waste Manag;61:220-228, 2017 Mar.
[Is] ISSN:1879-2456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Methane (CH ) and oxygen (air) concentrations affect the CH oxidation capacity (MOC) and mixed methanotrophic community structures in compost (fresh) and landfill (age old) top cover soils. A change in the mixed methanotrophic community structure in response has implications for landfill CH bio-filter remediation and possible bio-product outcomes (i.e., fatty acid methyl esters (FAME) content and profiles and polyhydroxybutyrate (PHB) contents). Therefore the study aimed to evaluate the effect of variable CH to oxygen ratios (10-50% CH in air) on mixed methanotrophic community structures enriched from landfill top cover (LB) and compost soils (CB) and to quantify flow on impacts on MOC, total FAME contents and profiles, and PHB accumulation. A stable consortium developed achieving average MOCs of 3.0±0.12, 4.1±0.26, 6.9±0.7, 7.6±1.3 and 9.2±1.2mgCH g DW h in LB and 2.9±0.04, 5.05±0.32, 6.7±0.31, 7.9±0.61 and 8.6±0.48mgCH g DW h in CB for a 20day cultivation period at 10, 20, 30, 40 and 50% CH , respectively. CB at 10% CH had a maximal FAME content of 40.5±0.8mgFAMEg DW , while maximal PHB contents (25mgg DW ) was observed at 40% CH in LB. Despite variable CH /O ratios, the mixed methanotrophic community structures in both LB and CB were relatively stable, dominated by Methylosarcina sp., and Chryseobacterium, suggesting that a resilient consortium had formed which can now be tested in bio-filter operations for CH mitigations in landfills.
[Mh] Termos MeSH primário: Metano/metabolismo
Consórcios Microbianos/fisiologia
Oxigênio/metabolismo
Microbiologia do Solo
[Mh] Termos MeSH secundário: Austrália
Chryseobacterium/genética
Chryseobacterium/metabolismo
Ácidos Graxos/química
Ácidos Graxos/metabolismo
Methylococcaceae/genética
Methylococcaceae/metabolismo
Consórcios Microbianos/genética
Oxirredução
Poliésteres/metabolismo
RNA Ribossômico 16S
Instalações de Eliminação de Resíduos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Polyesters); 0 (RNA, Ribosomal, 16S); OP0UW79H66 (Methane); S88TT14065 (Oxygen)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


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[PMID]:27830294
[Au] Autor:Zhao FJ; Liu Y; Pei XQ; Guo C; Wu ZL
[Ad] Endereço:Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.
[Ti] Título:Single mutations of ketoreductase ChKRED20 enhance the bioreductive production of (1S)-2-chloro-1-(3, 4-difluorophenyl) ethanol.
[So] Source:Appl Microbiol Biotechnol;101(5):1945-1952, 2017 Mar.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:(1S)-2-chloro-1-(3, 4-difluorophenyl) ethanol ((S)-CFPL) is an intermediate for the drug ticagrelor, and is manufactured via chemical approaches. To develop a biocatalytic solution to (S)-CFPL, an inventory of ketoreductases from Chryseobacterium sp. CA49 were rescreened, and ChKRED20 was found to catalyze the reduction of the ketone precursor with excellent stereoselectivity (>99 % ee). After screening an error-prone PCR library of the wild-type ChKRED20, two mutants, each bearing a single amino acid substitution of H145L or L205M, were identified with significantly increased activity. Then, the two critical positions were each randomized by constructing saturation mutagenesis libraries, which delivered several mutants with further enhanced activity. Among them, the mutant L205A was the best performer with a specific activity of 178 µmol/min/mg, ten times of that of the wild-type. Its k /K increased by 15 times and half-life at 50 °C increased by 70 %. The mutant catalyzed the complete conversion of 150 and 200 g/l substrate within 6 and 20 h, respectively, to yield enantiopure (S)-CFPL with an isolated yield of 95 %.
[Mh] Termos MeSH primário: Adenosina/análogos & derivados
Chryseobacterium/enzimologia
Etanol/análogos & derivados
Etanol/síntese química
Cetonas/metabolismo
Oxirredutases/metabolismo
[Mh] Termos MeSH secundário: 2-Propanol/química
Adenosina/síntese química
Adenosina/química
Biocatálise
Chryseobacterium/metabolismo
Etanol/química
Biblioteca Gênica
Mutagênese
NAD/química
Oxirredução
Oxirredutases/genética
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ketones); 0U46U6E8UK (NAD); 3K9958V90M (Ethanol); EC 1.- (Oxidoreductases); GLH0314RVC (Ticagrelor); K72T3FS567 (Adenosine); ND2M416302 (2-Propanol)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-016-7947-0



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