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[PMID]:28605957
[Au] Autor:Gyuris É; Wehmann E; Czeibert K; Magyar T
[Ad] Endereço:Laboratory of Domestic Mammal, Wildlife and Poultry Diseases, National Food Chain Safety Office - Veterinary Diagnostic Directorate , Budapest , Hungary.
[Ti] Título:Antimicrobial susceptibility of Riemerella anatipestifer strains isolated from geese and ducks in Hungary.
[So] Source:Acta Vet Hung;65(2):153-165, 2017 06.
[Is] ISSN:0236-6290
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:Riemerella anatipestifer causes anatipestifer disease in many avian species. A total of 185 R. anatipestifer strains isolated in Hungary between 2000 and 2014 from geese and ducks were tested against 13 antibiotics (ampicillin, doxycycline, enrofloxacin, erythromycin, florfenicol, flumequine, gentamicin, penicillin, spectinomycin, streptomycin, sulphamethoxazole-trimethoprim, sulphonamide compounds, and tetracycline) by the Kirby-Bauer disk diffusion method. The majority of the strains were susceptible to florfenicol (97.9%), ampicillin (95.1%), penicillin (93%), sulphamethoxazole-trimethoprim (92.4%), and spectinomycin (86.5%). The highest resistance rates were observed for flumequine, tetracycline, erythromycin and streptomycin (94%, 91.4%, 75.1% and 71.4% resistance, respectively). The resistance patterns showed some variation depending on the geographical origin of the strains. The average rate of extensive drug resistance was 30.3%, and its proportion tended to increase in the period examined.
[Mh] Termos MeSH primário: Anseriformes
Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Doenças das Aves Domésticas/microbiologia
Riemerella/classificação
Riemerella/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Hungria/epidemiologia
Doenças das Aves Domésticas/epidemiologia
Riemerella/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.016


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[PMID]:28284604
[Au] Autor:Wang M; Zhang P; Zhu D; Wang M; Jia R; Chen S; Sun K; Yang Q; Wu Y; Chen X; Biville F; Cheng A; Liu M
[Ad] Endereço:Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Research Center of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Key Laboratory of Animal Disease and Human Health o
[Ti] Título:Identification of the ferric iron utilization gene B739_1208 and its role in the virulence of R. anatipestifer CH-1.
[So] Source:Vet Microbiol;201:162-169, 2017 Mar.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Riemerella anatipestifer is an important bacterial pathogen in ducks and causes heavy economic losses in the duck industry. However, the pathogensis of this bacterium is poorly understood. In this study, a putative outer membrane hemin receptor gene B739_1208 in R. anatipestifer CH-1 was deleted to determine the relationship between iron uptake and virulence. The R. anatipestifer CH-1ΔB739_1208 mutants grew significantly more slowly than the wild-type bacteria in TSB liquid medium. Further characterization revealed that the R. anatipestifer CH-1ΔB739_1208 mutants were deficient in iron uptake. Animal experiments indicated that the median lethal dose of the wild-type RA-CH-1 in ducklings was 3.89×10 , whereas the median lethal dose of the R. anatipestifer CH-1ΔB739_1208 mutant in ducklings was 5.68×10 . The median lethal dose of the complementation strain in ducklings was 9.84×10 . Additional analysis indicated that bacterial loads in the blood, liver, and brain tissues in the R. anatipestifer CH-1ΔB739_1208-infected ducklings were significantly decreased compared to those in the wild-type R. anatipestifer CH-1 infected ducklings. In a duck co-infection model with R. anatipestifer CH-1 and R. anatipestifer CH-1ΔB739_1208, the R. anatipestifer CH-1B739_1208 mutant was outcompeted by the wild-type R. anatipestifer CH-1 in the blood (P<0.002), livers (P<0.001) and brains (P<0.001) of infected ducks, indicating that B739_1208 gene expression provided a competitive advantage in these organs. Our results demonstrate that the B739_1208 gene is a virulence factor in R. anatipestifer CH-1.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Patos/microbiologia
Infecções por Flavobacteriaceae/veterinária
Ferro/metabolismo
Doenças das Aves Domésticas/microbiologia
Riemerella/genética
[Mh] Termos MeSH secundário: Animais
Bacteriemia
Carga Bacteriana
Proteínas de Bactérias/metabolismo
Transporte Biológico
Encéfalo/microbiologia
Infecções por Flavobacteriaceae/microbiologia
Fígado/microbiologia
Mutação
Riemerella/metabolismo
Riemerella/patogenicidade
Virulência
Fatores de Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Virulence Factors); E1UOL152H7 (Iron)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


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[PMID]:28258143
[Au] Autor:Liu M; Zhang L; Huang L; Biville F; Zhu D; Wang M; Jia R; Chen S; Sun K; Yang Q; Wu Y; Chen X; Cheng A
[Ad] Endereço:Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, People's Republic of China liumafengra@163.com chenganchun@vip.163.com.
[Ti] Título:Use of Natural Transformation To Establish an Easy Knockout Method in Riemerella anatipestifer.
[So] Source:Appl Environ Microbiol;83(9), 2017 May 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:is a member of the family and a major causative agent of duck serositis. Little is known about its genetics and pathogenesis. Several bacteria are competent for natural transformation; however, whether is also competent for natural transformation has not been investigated. Here, we showed that strain ATCC 11845 can uptake the chromosomal DNA of strain RA-CH-1 in all growth phases. Subsequently, a natural transformation-based knockout method was established for ATCC 11845. Targeted mutagenesis gave transformation frequencies of ∼10 transformants. Competition assay experiments showed that ATCC 11845 preferentially took up its own DNA rather than heterogeneous DNA, such as DNA. Transformation was less efficient with the shuttle plasmid pLMF03 (transformation frequencies of ∼10 transformants). However, the efficiency of transformation was increased approximately 100-fold using pLMF03 derivatives containing DNA fragments (transformation frequencies of ∼10 transformants). Finally, we found that the RA-CH-1 strain was also naturally transformable, suggesting that natural competence is widely applicable for this species. The findings described here provide important tools for the genetic manipulation of is an important duck pathogen that belongs to the family At least 21 different serotypes have been identified. Genetic diversity has been demonstrated among these serotypes. The genetic and pathogenic mechanisms of remain largely unknown because no genetic tools are available for this bacterium. At present, natural transformation has been found in some bacteria but not in For the first time, we showed that natural transformation occurred in ATCC 11845 and RA-CH-1. Then, we established an easy gene knockout method in based on natural transformation. This information is important for further studies of the genetic diversity and pathogenesis in .
[Mh] Termos MeSH primário: Técnicas de Inativação de Genes/métodos
Genética Microbiana/métodos
Riemerella/genética
Transformação Bacteriana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


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[PMID]:28166822
[Au] Autor:Dou Y; Wang X; Yu G; Wang S; Tian M; Qi J; Li T; Ding C; Yu S
[Ad] Endereço:Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
[Ti] Título:Disruption of the M949_RS01915 gene changed the bacterial lipopolysaccharide pattern, pathogenicity and gene expression of Riemerella anatipestifer.
[So] Source:Vet Res;48(1):6, 2017 Feb 06.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Riemerella anatipestifer is an important pathogen that causes septicemia anserum exsudativa in ducks. Lipopolysaccharide (LPS) is considered to be a major virulence factor of R. anatipestifer. To identify genes involved in LPS biosynthesis, we screened a library of random Tn4351 transposon mutants using a monoclonal antibody against R. anatipestifer serotype 1 LPS (anti-LPS MAb). A mutant strain RA1067 which lost the reactivity in an indirect ELISA was obtained. Southern blot and sequencing analyses indicated a single Tn4351 was inserted at 116 bp in the M949_RS01915 gene in the RA1067 chromosomal DNA. Silver staining and Western blot analyses indicated that the RA1067 LPS was defected compared to the wild-type strain CH3 LPS. The RA1067 displayed a significant decreased growth rate at the late stage of growth in TSB in comparison with CH3. In addition, RA1067 showed higher susceptibility to complement-dependent killing, more than 360-fold attenuated virulence based on the median lethal dose determination, increased bacterial adhesion and invasion capacities to Vero cells and significantly decreased blood bacterial loads in RA1067 infected ducks, when compared to the CH3. An animal experiment indicated that inactivated RA1067 cells was effective in cross-protecting of the ducks from challenging with R. anatipestifer strains WJ4 (serotype 1), Yb2 (serotype 2) and HXb2 (serotype 10), further confirming the alteration of the RA1067 antigenicity. Moreover, RNA-Seq analysis and real-time PCR verified two up-regulated and three down-regulated genes in RA1067. Our findings demonstrate that the M949_RS01915 gene is associated to bacterial antigenicity, pathogenicity and gene regulation of R. anatipestifer.
[Mh] Termos MeSH primário: Patos/microbiologia
Lipopolissacarídeos/genética
Doenças das Aves Domésticas/microbiologia
Riemerella/patogenicidade
[Mh] Termos MeSH secundário: Animais
Western Blotting/veterinária
Ensaio de Imunoadsorção Enzimática/veterinária
Infecções por Flavobacteriaceae
Regulação Bacteriana da Expressão Gênica/genética
Genes Bacterianos/genética
Lipopolissacarídeos/isolamento & purificação
Lipopolissacarídeos/metabolismo
Mutação/genética
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Riemerella/genética
Riemerella/metabolismo
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipopolysaccharides); 0 (Virulence Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-017-0409-6


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[PMID]:27829415
[Au] Autor:Li Y; Zhang Y; Ding H; Mei X; Liu W; Zeng J; Zeng Z
[Ad] Endereço:Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
[Ti] Título:In vitro susceptibility of four antimicrobials against Riemerella anatipestifer isolates: a comparison of minimum inhibitory concentrations and mutant prevention concentrations for ceftiofur, cefquinome, florfenicol, and tilmicosin.
[So] Source:BMC Vet Res;12(1):250, 2016 Nov 09.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mutant prevention concentration (MPC) is an alternative pharmacodynamic parameter that has been used to measure antimicrobial activity and represents the propensities of antimicrobial agents to select resistant mutants. The concentration range between minimum inhibitory concentration (MIC) and MPC is defined as mutant selection window (MSW). The MPC and MSW parameters represent the ability of antimicrobial agents to inhibit the bacterial mutants selected. This study was conducted to determine the MIC and MPC values of four antimicrobials including ceftiofur, cefquinome, florfenicol and tilmicosin against 105 Riemerella anatipestifer isolates. RESULTS: The MIC /MIC values of clinical isolates tested in our study for ceftiofur, cefquinome, florfenicol and tilmicosin were 0.063/0.5、0.031/0.5、1/4、1/4 µg/mL, respectively; MPC / MPC values were 4/64、8/64、4/32、16/256 µg/mL, respectively. These results provided information on the use of these compounds in treating the R. anatipestifer infection; however, additional studies are needed to demonstrate their therapeutic efficacy. CONCLUSION: Based on the MSW theory, the hierarchy of these tested antimicrobial agents with respect to selecting resistant subpopulations was as follows: cefquinome > ceftiofur > tilmicosin > florfenicol. Cefquinome was the drug that presented the highest risk of selecting resistant mutant among the four antimicrobial agents.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Cefalosporinas/farmacologia
Riemerella/efeitos dos fármacos
Tianfenicol/análogos & derivados
Tilosina/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacocinética
Cefalosporinas/farmacocinética
Farmacorresistência Bacteriana/genética
Patos/microbiologia
Infecções por Flavobacteriaceae/microbiologia
Infecções por Flavobacteriaceae/veterinária
Gansos/microbiologia
Testes de Sensibilidade Microbiana
Mutação
Doenças das Aves Domésticas/microbiologia
Riemerella/genética
Riemerella/isolamento & purificação
Tianfenicol/farmacocinética
Tianfenicol/farmacologia
Tilosina/farmacocinética
Tilosina/farmacologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephalosporins); 83JL932I1C (ceftiofur); 9J97307Y1H (florfenicol); FLQ7571NPM (Thiamphenicol); XL4103X2E3 (tilmicosin); YEF4JXN031 (Tylosin); Z74S078CWP (cefquinome)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE


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[PMID]:27577199
[Au] Autor:Zhu DK; Yang XQ; He Y; Zhou WS; Song XH; Wang JB; Zhang Y; Liu MF; Wang MS; Jia RY; Chen S; Sun KF; Yang Q; Wu Y; Chen XY; Cheng AC
[Ad] Endereço:Research Center of Avian Diseases, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan, China.
[Ti] Título:Comparative genomic analysis identifies structural features of CRISPR-Cas systems in Riemerella anatipestifer.
[So] Source:BMC Genomics;17:689, 2016 08 30.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Riemerella anatipestifer infection is a contagious disease that has resulted in major economic losses in the duck industry worldwide. This study attempted to characterize CRISPR-Cas systems in the disease-causing agent, Riemerella anatipestifer (R. anatipestifer). The CRISPR-Cas system provides adaptive immunity against foreign genetic elements in prokaryotes and CRISPR-cas loci extensively exist in the genomes of archaea and bacteria. However, the structure characteristics of R. anatipestifer CRISPR-Cas systems remains to be elucidated due to the limited availability of genomic data. RESULTS: To identify the structure and components associated with CRISPR-Cas systems in R. anatipestifer, we performed comparative genomic analysis of CRISPR-Cas systems in 25 R. anatipestifer strains using high-throughput sequencing. The results showed that most of the R. anatipestifer strains (20/25) that were analyzed have two CRISPR loci (CRISPR1 and CRISPR2). CRISPR1 was shown to be flanked on one side by cas genes, while CRISPR2 was designated as an orphan. The other analyzed strains harbored only one locus, either CRISPR1 or CRISPR2. The length and content of consensus direct repeat sequences, as well as the length of spacer sequences associated with the two loci, differed from each other. Only three cas genes (cas1, cas2 and cas9) were located upstream of CRISPR1. CRISPR1 was also shown to be flanked by a 107 bp-long putative leader sequence and a 16 nt-long anti-repeat sequence. Combined with analysis of spacer organization similarity and phylogenetic tree of the R. anatipestifer strains, CRISPR arrays can be divided into different subgroups. The diversity of spacer organization was observed in the same subgroup. In general, spacer organization in CRISPR1 was more divergent than that in CRISPR2. Additionally, only 8 % of spacers (13/153) were homologous with phage or plasmid sequences. The cas operon flanking CRISPR1 was observed to be relatively conserved based on multiple sequence alignments of Cas amino acid sequences. The phylogenetic analysis associated with Cas9 showed Cas9 sequence from R. anatipestifer was closely related to that of Bacteroides fragilis and formed part of the subtype II-C subcluster. CONCLUSIONS: Our data revealed for the first time the structural features of R. anatipestifer CRISPR-Cas systems. The illumination of structural features of CRISPR-Cas system may assist in studying the specific mechanism associated with CRISPR-mediated adaptive immunity and other biological functions in R. anatipestifer.
[Mh] Termos MeSH primário: Sistemas CRISPR-Cas/genética
Filogenia
Riemerella/genética
[Mh] Termos MeSH secundário: Hibridização Genômica Comparativa
Variação Genética
Genômica
Plasmídeos/genética
Riemerella/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160901
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-3040-4


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[PMID]:27517915
[Au] Autor:Liu J; Zhu D; Ma G; Liu M; Wang M; Jia R; Chen S; Sun K; Yang Q; Wu Y; Chen X; Cheng A
[Ad] Endereço:Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China. eaeas12@163.com.
[Ti] Título:Genome-Wide Analysis of the Synonymous Codon Usage Patterns in Riemerella anatipestifer.
[So] Source:Int J Mol Sci;17(8), 2016 Aug 10.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Riemerella anatipestifer (RA) belongs to the Flavobacteriaceae family and can cause a septicemia disease in poultry. The synonymous codon usage patterns of bacteria reflect a series of evolutionary changes that enable bacteria to improve tolerance of the various environments. We detailed the codon usage patterns of RA isolates from the available 12 sequenced genomes by multiple codon and statistical analysis. Nucleotide compositions and relative synonymous codon usage (RSCU) analysis revealed that A or U ending codons are predominant in RA. Neutrality analysis found no significant correlation between GC12 and GC3 (p > 0.05). Correspondence analysis and ENc-plot results showed that natural selection dominated over mutation in the codon usage bias. The tree of cluster analysis based on RSCU was concordant with dendrogram based on genomic BLAST by neighbor-joining method. By comparative analysis, about 50 highly expressed genes that were orthologs across all 12 strains were found in the top 5% of high CAI value. Based on these CAI values, we infer that RA contains a number of predicted highly expressed coding sequences, involved in transcriptional regulation and metabolism, reflecting their requirement for dealing with diverse environmental conditions. These results provide some useful information on the mechanisms that contribute to codon usage bias and evolution of RA.
[Mh] Termos MeSH primário: Códon/genética
Genoma Bacteriano/genética
Riemerella/genética
[Mh] Termos MeSH secundário: Mutação
Seleção Genética/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160813
[St] Status:MEDLINE


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[PMID]:27500736
[Au] Autor:Yu G; Wang X; Dou Y; Wang S; Tian M; Qi J; Li T; Ding C; Wu Y; Yu S
[Ad] Endereço:Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
[Ti] Título:Riemerella anatipestifer M949_1360 Gene Functions on the Lipopolysaccharide Biosynthesis and Bacterial Virulence.
[So] Source:PLoS One;11(8):e0160708, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Riemerella anatipestifer causes septicemic and exudative diseases in poultry, resulting in major economic losses to the duck industry. Lipopolysaccharide (LPS), as an important virulence factor in Gram-negative bacteria, can be recognized by the immune system and plays a crucial role in many interactions between bacteria and animal hosts. In this study, we screened out one LPS defective mutant strain RAΔ604 from a random transposon mutant library of R. anatipestifer serotype 1 strain CH3, which did not react with the anti-CH3 LPS monoclonal antibody 1C1 in an indirect enzyme-linked immunosorbent assay. Southern blot analysis confirmed that the genome of RAΔ604 contained a single Tn4351 insert. Then, we found that the M949_1360 gene was inactivated by insertion of the transposon. Using silver staining and western blot analyses, we found that the LPS pattern of RAΔ604 was defective, as compared with that of the wild-type (WT) strain CH3. The mutant strain RAΔ604 showed no significant influence on bacterial growth, while bacterial counting and Live/dead BacLight Bacterial Viability staining revealed that bacterial viability was decreased, as compared with the WT strain CH3. In addition, the abilities of the mutant strain RAΔ604 to adhere and invade Vero cells were significantly decreased. Animal studies revealed that the virulence of the mutant strain RAΔ604 was decreased by more than 200-fold in a duck infection model, as compared with the WT strain CH3. Furthermore, immunization with live bacteria of the mutant strain RAΔ604 protected 87.5% ducks from challenge with R. anatipestifer serotype 1 strain WJ4, indicating that the mutant strain RAΔ604 could be used as a potential vaccine candidate in the future.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Infecções por Flavobacteriaceae/veterinária
Lipopolissacarídeos/biossíntese
Riemerella/genética
Riemerella/patogenicidade
Fatores de Virulência/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Patos
Ensaio de Imunoadsorção Enzimática
Infecções por Flavobacteriaceae/imunologia
Infecções por Flavobacteriaceae/microbiologia
Camundongos
Camundongos Endogâmicos BALB C
Doenças das Aves Domésticas/imunologia
Doenças das Aves Domésticas/microbiologia
Riemerella/metabolismo
Virulência
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Lipopolysaccharides); 0 (Virulence Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160809
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0160708


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[PMID]:27451449
[Au] Autor:Wang X; Liu B; Dou Y; Fan H; Wang S; Li T; Ding C; Yu S
[Ad] Endereço:Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, People's Republic of China.
[Ti] Título:The Riemerella anatipestifer AS87_01735 Gene Encodes Nicotinamidase PncA, an Important Virulence Factor.
[So] Source:Appl Environ Microbiol;82(19):5815-23, 2016 Oct 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Riemerella anatipestifer is a major bacterial pathogen that causes septicemic and exudative diseases in domestic ducks. In our previous study, we found that deletion of the AS87_01735 gene significantly decreased the bacterial virulence of R. anatipestifer strain Yb2 (mutant RA625). The AS87_01735 gene was predicted to encode a nicotinamidase (PncA), a key enzyme that catalyzes the conversion of nicotinamide to nicotinic acid, which is an important reaction in the NAD(+) salvage pathway. In this study, the AS87_01735 gene was expressed and identified as the PncA-encoding gene, using an enzymatic assay. Western blot analysis demonstrated that R. anatipestifer PncA was localized to the cytoplasm. The mutant strain RA625 (named Yb2ΔpncA in this study) showed a similar growth rate but decreased NAD(+) quantities in both the exponential and stationary phases in tryptic soy broth culture, compared with the wild-type strain Yb2. In addition, Yb2ΔpncA-infected ducks showed much lower bacterial loads in their blood, and no visible histological changes were observed in the heart, liver, and spleen. Furthermore, Yb2ΔpncA immunization of ducks conferred effective protection against challenge with the virulent wild-type strain Yb2. Our results suggest that the R. anatipestifer AS87_01735 gene encodes PncA, which is an important virulence factor, and that the Yb2ΔpncA mutant can be used as a novel live vaccine candidate. IMPORTANCE: Riemerella anatipestifer is reported worldwide as a cause of septicemic and exudative diseases of domestic ducks. The pncA gene encodes a nicotinamidase (PncA), a key enzyme that catalyzes the conversion of nicotinamide to nicotinic acid, which is an important reaction in the NAD(+) salvage pathway. In this study, we identified and characterized the pncA-homologous gene AS87_01735 in R. anatipestifer strain Yb2. R. anatipestifer PncA is a cytoplasmic protein that possesses similar PncA activity, compared with other organisms. Generation of the pncA mutant Yb2ΔpncA led to a decrease in the NAD(+) content, which was associated with decreased capacity for invasion and attenuated virulence in ducks. Furthermore, Yb2ΔpncA immunization of ducks conferred effective protection against challenge with the virulent wild-type strain Yb2. Altogether, these results suggest that PncA contributes to the virulence of R. anatipestifer and that the Yb2ΔpncA mutant can be used as a novel live vaccine candidate.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Patos
Infecções por Flavobacteriaceae/veterinária
Nicotinamidase/genética
Doenças das Aves Domésticas/imunologia
Riemerella/genética
Fatores de Virulência/genética
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/imunologia
Proteínas de Bactérias/metabolismo
Vacinas Bacterianas/imunologia
Vacinas Bacterianas/microbiologia
Infecções por Flavobacteriaceae/imunologia
Infecções por Flavobacteriaceae/microbiologia
Expressão Gênica
Imunização/veterinária
Nicotinamidase/imunologia
Nicotinamidase/metabolismo
Doenças das Aves Domésticas/microbiologia
Riemerella/imunologia
Riemerella/metabolismo
Deleção de Sequência
Vacinas Atenuadas/imunologia
Fatores de Virulência/imunologia
Fatores de Virulência/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Vaccines); 0 (Vaccines, Attenuated); 0 (Virulence Factors); EC 3.5.1.19 (Nicotinamidase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01829-16


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Fotocópia
[PMID]:27373315
[Au] Autor:Chikuba T; Uehara H; Fumikura S; Takahashi K; Suzuki Y; Hoshinoo K; Yamamoto Y
[Ad] Endereço:Mie Chuo Livestock Hygiene Service Center, 1742-1 Ishinden-Kozubeta, Tsu, Mie 514-0061, Japan.
[Ti] Título:Riemerella anatipestifer infection in domestic ducks in Japan, 2014.
[So] Source:J Vet Med Sci;78(10):1635-1638, 2016 Nov 01.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The outbreak of Riemerella anatipestifer (RA) infection has been confirmed in meat-type domestic ducks (Anas platyrhynchos) for the first time in 27 years in Japan. In January 2014, increased mortality in a 14- to 21-day-old duck flock was reported to veterinary officials by the owner. The affected ducks exhibited reduced movement, ataxia and dorsal recumbency with leg paddling. Pathological findings were typical for an RA infection. Fibrinous and heterophilic pericarditis, airsacculitis, perihepatitis, ventriculitis and meningitis were observed. The bacterial isolate from duck organs was identified as RA by PCR-based 16S ribosomal RNA sequencing.
[Mh] Termos MeSH primário: Patos
Infecções por Flavobacteriaceae/veterinária
Doenças das Aves Domésticas/microbiologia
Riemerella
[Mh] Termos MeSH secundário: Animais
Surtos de Doenças/veterinária
Infecções por Flavobacteriaceae/epidemiologia
Infecções por Flavobacteriaceae/patologia
Japão/epidemiologia
Doenças das Aves Domésticas/epidemiologia
Doenças das Aves Domésticas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE



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