Base de dados : MEDLINE
Pesquisa : B03.250 [Categoria DeCS]
Referências encontradas : 409 [refinar]
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[PMID]:29364601
[Au] Autor:Lunina ON; Savvichev AS; Krasnova ED; Kokryatskaya NM; Veslopolova EF; Kuznetsov BB; Gorlenko VM
[Ti] Título:Succession Processes in the Anoxygenic Phototrophic Bacterial Community in Lake Kislo-Sladkoe (Kandalaksha Bay, White Sea).
[So] Source:Mikrobiologiia;85(5):531-544, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The community of anoxygenic phototrophic bacteria (APB) in the water column of Lake Kislo- Sladkoe (Kandalaksha Bay, White Sea), which has recently become separated from the sea, was investigated in March-April 2012, March-April 2013, and in September 2013. The lake, which was previously considered meromictic, was in fact mixed and was strongly affected by the sea. In winter the lake is sometimes washed off with seawater, and this together with the seasonal cycles of succession processes determines the succession of the community. The consequences of the mixing in autumn 2011 could be observed in the APB community as late as autumn 2013. Green-colored green sulfur bacteria (GSB) usually predominated in the chemocline. In winter 2013 stagnation resulted in turbidity of water under the ice, which was responsible for both predom- inance of the brown GS B forms and the changes ratio of the species of purple sulfur bacteria (PS B) in anoxic water layers. Production of anoxygenic photosynthesis in the lake was at least 240 mg C m-2 day-- in September and 0-20 mg C m-2 day- in March-April, which corresponded to 40 and 69%, respectively, of oxygenic photosynthesis. Okenone-containing purple sulfur bacteria, strain TcakPS12 were isolated in 2012 from lake water. The ells of this strain form filaments of not separated cells. Strain TcakPS12 exhibited 98% similarity with the type strains of Thiocapsapendens DSM.236 and Thiocapsa bogorovii BBS, as well as with the strains AmPS10 and TcyrPS 10, which were isolated from Lake Kislo-Sladkoe in 2010.
[Mh] Termos MeSH primário: Baías/microbiologia
Chlorobi/genética
Chromatiaceae/genética
Lagos/microbiologia
Consórcios Microbianos/fisiologia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Chlorobi/classificação
Chlorobi/isolamento & purificação
Chlorobi/ultraestrutura
Chromatiaceae/classificação
Chromatiaceae/isolamento & purificação
Chromatiaceae/ultraestrutura
Ecossistema
Fotossíntese/fisiologia
Filogenia
Pigmentos Biológicos/isolamento & purificação
Federação Russa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pigments, Biological); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:29245157
[Au] Autor:Danza F; Storelli N; Roman S; Lüdin S; Tonolla M
[Ad] Endereço:Laboratory of Applied Microbiology (LMA), Department for Environmental Constructions and Design (DACD), University of Applied Sciences and Arts of Southern Switzerland (SUPSI), via Mirasole 22a, Bellinzona, Switzerland.
[Ti] Título:Dynamic cellular complexity of anoxygenic phototrophic sulfur bacteria in the chemocline of meromictic Lake Cadagno.
[So] Source:PLoS One;12(12):e0189510, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The meromictic Lake Cadagno is characterized by a compact chemocline with high concentrations of anoxygenic phototrophic purple sulfur bacteria (PSB) and green sulfur bacteria (GSB). The co-occurrence of phylogenetically distant bacterial groups such as PSB and GSB in the same ecological niche, makes the chemocline of Lake Cadagno an ideal system for studying the conditions and consequences of coexistence of photosynthetic bacteria populations. In this study, we applied flow cytometry (FCM) as a fast tool to identify metabolic changes due to the production and consumption of inclusion bodies such as sulfur globules (SGBs), and follow population dynamics of closely related anoxygenic photosynthetic sulfur bacteria in their natural environment. Large-celled PSB Chromatium okenii and GSB Chlorobium populations were reliably separated and identified due to differences in auto-fluorescence and cell size. Moreover, we showed that these dominant taxa share the same ecological niche over seasonal periods. Taking advantage of FCM detection of dynamic cellular complexity variation during phases of photosynthetic activity, we identified an unexpected alternation in PSB versus GSB metabolic activity, indicating dynamic interspecific interactions between these two populations.
[Mh] Termos MeSH primário: Chlorobi/fisiologia
Chromatiaceae/fisiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Anaerobiose
DNA Bacteriano/metabolismo
Lagos/análise
Lagos/microbiologia
Oxirredução
Fotossíntese
RNA Bacteriano/metabolismo
Estações do Ano
Sulfetos/análise
Sulfetos/metabolismo
Suíça
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (Sulfides)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189510


  3 / 409 MEDLINE  
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[PMID]:28035767
[Au] Autor:de Beer D; Weber M; Chennu A; Hamilton T; Lott C; Macalady J; M Klatt J
[Ad] Endereço:Microsensor Group, Max-Planck-Institute for Marine Microbiology, Celsiusstrasse 1, Bremen, 28359, Germany.
[Ti] Título:Oxygenic and anoxygenic photosynthesis in a microbial mat from an anoxic and sulfidic spring.
[So] Source:Environ Microbiol;19(3):1251-1265, 2017 Mar.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Oxygenic and anoxygenic photosynthesis were studied with microsensors in microbial mats found at 9-10 m depth in anoxic and sulfidic water in Little Salt Spring (Florida, USA). The lake sediments were covered with a 1-2 mm thick red mat dominated by filamentous Cyanobacteria, below which Green Sulfur Bacteria (GSB, Chlorobiaceae) were highly abundant. Within 4 mm inside the mats, the incident radiation was attenuated to undetectable levels. In situ microsensor data showed both oxygenic photosynthesis in the red surface layer and light-induced sulfide dynamics up to 1 cm depth. Anoxygenic photosynthesis occurred during all daylight hours, with complete sulfide depletion around midday. Oxygenic photosynthesis was limited to 4 h per day, due to sulfide inhibition in the early morning and late afternoon. Laboratory measurements on retrieved samples showed that oxygenic photosynthesis was fully but reversibly inhibited by sulfide. In patches Fe(III) alleviated the inhibition of oxygenic photosynthesis by sulfide. GSB were resistant to oxygen and showed a low affinity to sulfide. Their light response showed saturation at very low intensities.
[Mh] Termos MeSH primário: Chlorobi/metabolismo
Cianobactérias/metabolismo
Fontes Termais/microbiologia
Lagos/microbiologia
Oxigênio/metabolismo
Fotossíntese
Sulfetos/metabolismo
[Mh] Termos MeSH secundário: Chlorobi/classificação
Chlorobi/genética
Chlorobi/isolamento & purificação
Cianobactérias/genética
Cianobactérias/isolamento & purificação
Compostos Férricos/análise
Compostos Férricos/metabolismo
Florida
Fontes Termais/análise
Lagos/análise
Fotossíntese/fisiologia
Sulfetos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ferric Compounds); 0 (Sulfides); S88TT14065 (Oxygen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161231
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13654


  4 / 409 MEDLINE  
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[PMID]:27994052
[Au] Autor:Thweatt JL; Ferlez BH; Golbeck JH; Bryant DA
[Ad] Endereço:From the Departments of Biochemistry and Molecular Biology and.
[Ti] Título:BciD Is a Radical S-Adenosyl-l-methionine (SAM) Enzyme That Completes Bacteriochlorophyllide e Biosynthesis by Oxidizing a Methyl Group into a Formyl Group at C-7.
[So] Source:J Biol Chem;292(4):1361-1373, 2017 Jan 27.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Green bacteria are chlorophotorophs that synthesize bacteriochlorophyll (BChl) c, d, or e, which assemble into supramolecular, nanotubular structures in large light-harvesting structures called chlorosomes. The biosynthetic pathways of these chlorophylls are known except for one reaction. Null mutants of bciD, which encodes a putative radical S-adenosyl-l-methionine (SAM) protein, are unable to synthesize BChl e but accumulate BChl c; however, it is unknown whether BciD is sufficient to convert BChl c (or its precursor, bacteriochlorophyllide (BChlide) c) into BChl e (or BChlide e). To determine the function of BciD, we expressed the bciD gene of Chlorobaculum limnaeum strain DSMZ 1677 in Escherichia coli and purified the enzyme under anoxic conditions. Electron paramagnetic resonance spectroscopy of BciD indicated that it contains a single [4Fe-4S] cluster. In assays containing SAM, BChlide c or d, and sodium dithionite, BciD catalyzed the conversion of SAM into 5'-deoxyadenosine and BChlide c or d into BChlide e or f, respectively. Our analyses also identified intermediates that are proposed to be 7 -OH-BChlide c and d Thus, BciD is a radical SAM enzyme that converts the methyl group of BChlide c or d into the formyl group of BChlide e or f This probably occurs by a mechanism involving consecutive hydroxylation reactions of the C-7 methyl group to form a geminal diol intermediate, which spontaneously dehydrates to produce the final products, BChlide e or BChlide f The demonstration that BciD is sufficient to catalyze the conversion of BChlide c into BChlide e completes the biosynthetic pathways for all "Chlorobium chlorophylls."
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Bacterioclorofilas/biossíntese
Chlorobi/enzimologia
Proteínas com Ferro-Enxofre/metabolismo
Metionina Adenosiltransferase/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Bacterioclorofilas/genética
Chlorobi/genética
Proteínas com Ferro-Enxofre/genética
Metionina Adenosiltransferase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacteriochlorophylls); 0 (Iron-Sulfur Proteins); EC 2.5.1.6 (Methionine Adenosyltransferase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161221
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.767665


  5 / 409 MEDLINE  
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[PMID]:27862807
[Au] Autor:Savvichev AS; Kokryatskaya NM; Zabelina SA; Rusanov II; Zakharova EE; Veslopolova EF; Lunina ON; Patutina EO; Bumazhkin BK; Gruzdev DS; Sigalevich PA; Pimenov NV; Kuznetsov BB; Gorlenko VM
[Ad] Endereço:Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
[Ti] Título:Microbial processes of the carbon and sulfur cycles in an ice-covered, iron-rich meromictic lake Svetloe (Arkhangelsk region, Russia).
[So] Source:Environ Microbiol;19(2):659-672, 2017 Feb.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biogeochemical, isotope geochemical and microbiological investigation of Lake Svetloe (White Sea basin), a meromictic freshwater was carried out in April 2014, when ice thickness was ∼0.5 m, and the ice-covered water column contained oxygen to 23 m depth. Below, the anoxic water column contained ferrous iron (up to 240 µµM), manganese (60 µM), sulfide (up to 2 µM) and dissolved methane (960 µM). The highest abundance of microbial cells revealed by epifluorescence microscopy was found in the chemocline (redox zone) at 23-24.5 m. Oxygenic photosynthesis exhibited two peaks: the major one (0.43 µmol C L  day ) below the ice and the minor one in the chemocline zone, where cyanobacteria related to Synechococcus rubescens were detected. The maximum of anoxygenic photosynthesis (0.69 µmol C L  day ) at the oxic/anoxic interface, for which green sulfur bacteria Chlorobium phaeoclathratiforme were probably responsible, exceeded the value for oxygenic photosynthesis. Bacterial sulfate reduction peaked (1.5 µmol S L  day ) below the chemocline zone. The rates of methane oxidation were as high as 1.8 µmol CH  L  day at the oxi/anoxic interface and much lower in the oxic zone. Small phycoerythrin-containing Synechococcus-related cyanobacteria were probably involved in accumulation of metal oxides in the redox zone.
[Mh] Termos MeSH primário: Ciclo do Carbono
Carbono/metabolismo
Camada de Gelo
Ferro/química
Lagos/microbiologia
Enxofre/metabolismo
[Mh] Termos MeSH secundário: Dióxido de Carbono/análise
Chlorobi/metabolismo
Ecossistema
Lagos/química
Metano/análise
Oxirredução
Oxigênio
Fotossíntese
Federação Russa
Sulfetos
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sulfides); 142M471B3J (Carbon Dioxide); 70FD1KFU70 (Sulfur); 7440-44-0 (Carbon); E1UOL152H7 (Iron); OP0UW79H66 (Methane); S88TT14065 (Oxygen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13591


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[PMID]:27838020
[Au] Autor:Wan D; Liu Y; Wang Y; Wang H; Xiao S
[Ad] Endereço:School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China. Electronic address: dongjinwan@yeah.net.
[Ti] Título:Simultaneous bio-autotrophic reduction of perchlorate and nitrate in a sulfur packed bed reactor: Kinetics and bacterial community structure.
[So] Source:Water Res;108:280-292, 2017 Jan 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study investigated the simultaneous removal of perchlorate and nitrate from aqueous solution in an up-flow sulfur autotrophic reduction reactor. A nitrate and perchlorate containing pollution solution was treated with a remarkable removal efficiency greater than 97%. The concentration of nitrate was 22.03 ± 1.07 mg-N/L coexisting with perchlorate either 21.87 ± 1.03 mg/L or 471.7 ± 50.3 µg/L, in this case the reactor could be operated at a hydraulic retention time (HRT) ranging from 12.00 h to 0.75 h. Half-order kinetics model fit the experimental data well; this indicates that diffusion in the biofilm was the limiting step. Perchlorate reduction required a longer reaction time than the coexisting nitrate, regardless of the perchlorate concentration. Sulfur (S) disproportionation was inhibited when nitrate was not completely removed; whereas it was accelerated when perchlorate decreased to low concentrations. This process therefore generated excessive sulfate and consumed much more alkalinity. High-throughput sequencing method was used to analyze bacterial community spatial distribution in the reactor under different operational conditions. The reduction of the two contaminants was accompanied by a decrease in biodiversity. The results indicated that Sulfuricella, Sulfuritalea Thiobacillus, and Sulfurimonas are effective DB (denitrification bacteria)/PRB (perchlorate reduction bacteria). The Chlorobaculum genus was the dominant bacteria associated with S disproportionation.
[Mh] Termos MeSH primário: Percloratos
Enxofre/química
[Mh] Termos MeSH secundário: Reatores Biológicos/microbiologia
Chlorobi
Cinética
Nitratos/química
Purificação da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrates); 0 (Perchlorates); 70FD1KFU70 (Sulfur)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161114
[St] Status:MEDLINE


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[PMID]:27565613
[Au] Autor:Levy AT; Lee KH; Hanson TE
[Ad] Endereço:Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA.
[Ti] Título:Chlorobaculum tepidum Modulates Amino Acid Composition in Response to Energy Availability, as Revealed by a Systematic Exploration of the Energy Landscape of Phototrophic Sulfur Oxidation.
[So] Source:Appl Environ Microbiol;82(21):6431-6439, 2016 Nov 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microbial sulfur metabolism, particularly the formation and consumption of insoluble elemental sulfur (S ), is an important biogeochemical engine that has been harnessed for applications ranging from bioleaching and biomining to remediation of waste streams. Chlorobaculum tepidum, a low-light-adapted photoautolithotrophic sulfur-oxidizing bacterium, oxidizes multiple sulfur species and displays a preference for more reduced electron donors: sulfide > S > thiosulfate. To understand this preference in the context of light energy availability, an "energy landscape" of phototrophic sulfur oxidation was constructed by varying electron donor identity, light flux, and culture duration. Biomass and cellular parameters of C. tepidum cultures grown across this landscape were analyzed. From these data, a correction factor for colorimetric protein assays was developed, enabling more accurate biomass measurements for C. tepidum, as well as other organisms. C. tepidum's bulk amino acid composition correlated with energy landscape parameters, including a tendency toward less energetically expensive amino acids under reduced light flux. This correlation, paired with an observation of increased cell size and storage carbon production under electron-rich growth conditions, suggests that C. tepidum has evolved to cope with changing energy availability by tuning its proteome for energetic efficiency and storing compounds for leaner times. IMPORTANCE: How microbes cope with and adapt to varying energy availability is an important factor in understanding microbial ecology and in designing efficient biotechnological processes. We explored the response of a model phototrophic organism, Chlorobaculum tepidum, across a factorial experimental design that enabled simultaneous variation and analysis of multiple growth conditions, what we term the "energy landscape." C. tepidum biomass composition shifted toward less energetically expensive amino acids at low light levels. This observation provides experimental evidence for evolved efficiencies in microbial proteomes and emphasizes the role that energy flux may play in the adaptive responses of organisms. From a practical standpoint, our data suggest that bulk biomass amino acid composition could provide a simple proxy to monitor and identify energy stress in microbial systems.
[Mh] Termos MeSH primário: Aminoácidos/química
Chlorobi/metabolismo
Processos Fototróficos
Enxofre/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/metabolismo
Biomassa
Chlorobi/crescimento & desenvolvimento
Elétrons
Luz
Oxirredução
Proteoma
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Proteome); 70FD1KFU70 (Sulfur)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE


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[PMID]:27427396
[Au] Autor:Saga Y; Yamashita H; Hirota K
[Ad] Endereço:Department of Chemistry, Faculty of Science and Engineering, Kindai University, Higashi-Osaka, Osaka 577-8502, Japan; PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan. Electronic address: saga@chem.kindai.ac.jp.
[Ti] Título:Introduction of perfluoroalkyl chain into the esterifying moiety of bacteriochlorophyll c in the green sulfur photosynthetic bacterium Chlorobaculum tepidum by pigment biosynthesis.
[So] Source:Bioorg Med Chem;24(18):4165-4170, 2016 Sep 15.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The green sulfur photosynthetic bacterium Chlorobaculum (Cba.) tepidum was grown in liquid cultures containing perfluoro-1-decanol, 1H,1H,2H,2H-heptadecafluoro-1-decanol [CF3(CF2)7(CH2)2OH] or 1H,1H-nonadecafluoro-1-decanol [CF3(CF2)8CH2OH], to introduce rigid and fluorophilic chains into the esterifying moiety of light-harvesting bacteriochlorophyll (BChl) c. Exogenous 1H,1H,2H,2H-heptadecafluoro-1-decanol was successfully attached to the 17(2)-carboxy group of bacteriochlorophyllide (BChlide) c in vivo: the relative ratio of the unnatural BChl c esterified with this perfluoroalcohol over the total BChl c was 10.3%. Heat treatment of the liquid medium containing 1H,1H,2H,2H-heptadecafluoro-1-decanol with ß-cyclodextrin before inoculation increased the relative ratio of the BChl c derivative esterified with this alcohol in the total BChl c in Cba. tepidum. In a while, 1H,1H-nonadecafluoro-1-decanol was not attached to BChlide c in Cba. tepidum, which was grown by its supplementation. These results suggest that the rigidity close to the hydroxy group of the esterifying alcohol is not suitable for the recognition by the BChl c synthase called BchK in Cba. tepidum. The unnatural BChl c esterified with 1H,1H,2H,2H-heptadecafluoro-1-decanol participated in BChl c self-aggregates in chlorosomes.
[Mh] Termos MeSH primário: Proteínas de Bactérias/biossíntese
Bacterioclorofilas/biossíntese
Chlorobi/metabolismo
Fluorcarbonetos/química
Pigmentos Biológicos/biossíntese
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/isolamento & purificação
Bacterioclorofilas/química
Bacterioclorofilas/isolamento & purificação
Ésteres/química
Ésteres/metabolismo
Álcoois Graxos/química
Complexos de Proteínas Captadores de Luz/química
Pigmentos Biológicos/química
Pigmentos Biológicos/isolamento & purificação
Temperatura Ambiente
beta-Ciclodextrinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacteriochlorophylls); 0 (Esters); 0 (Fatty Alcohols); 0 (Fluorocarbons); 0 (Light-Harvesting Protein Complexes); 0 (Pigments, Biological); 0 (beta-Cyclodextrins); 53986-51-9 (bacteriochlorophyll c); JV039JZZ3A (betadex); UM7L66OFE1 (1H,1H,2H,2H-perfluorodecanol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170812
[Lr] Data última revisão:
170812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160719
[St] Status:MEDLINE


  9 / 409 MEDLINE  
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[PMID]:27406896
[Au] Autor:Mackowski S; Czechowski N; Ashraf KU; Szalkowski M; Lokstein H; Cogdell RJ; Kowalska D
[Ad] Endereço:Optics of Hybrid Nanostructures Group, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Torun, Poland.
[Ti] Título:Origin of bimodal fluorescence enhancement factors of Chlorobaculum tepidum reaction centers on silver island films.
[So] Source:FEBS Lett;590(16):2558-65, 2016 Aug.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We focus on the spectral dependence of plasmon-induced enhancement of fluorescence of Chlorobaculum tepidum reaction centers. When deposited on silver island film, they exhibit up to a 60-fold increase in fluorescence. The dependence of enhancement factors on the excitation wavelength is not correlated with the absorption spectrum of the plasmonic structure. In particular, the presence of one (or multiple) trimers of the Fenna-Matthews-Olson (FMO) protein reveals itself in bimodal distribution of enhancement factors for the excitation at 589 nm, the wavelength corresponding to bacteriochlorophyll absorption of FMO and the core of the RC. We conclude that the structure of multichromophoric complexes can substantially affect the impact of plasmonic excitations, which is important in the context of assembling functional biohybrid systems.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Chlorobi/química
Citoplasma/química
Fluorescência
Complexos de Proteínas Captadores de Luz/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Chlorobi/genética
Chlorobi/metabolismo
Citoplasma/genética
Transferência de Energia/genética
Complexos de Proteínas Captadores de Luz/genética
Espectrometria de Fluorescência
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (FMO bacteriochlorophyll protein, Bacteria); 0 (Light-Harvesting Protein Complexes)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160714
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12292


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[PMID]:27190141
[Au] Autor:Shuman KE; Hanson TE
[Ad] Endereço:Department of Biological Sciences, University of Delaware, Newark, DE 19711, USA Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA.
[Ti] Título:A sulfide:quinone oxidoreductase from Chlorobaculum tepidum displays unusual kinetic properties.
[So] Source:FEMS Microbiol Lett;363(12), 2016 06.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sulfide:quinone oxidoreductase (SQR) is the primary sulfide-oxidizing enzyme found in all three domains of life. Of the six phylogenetically distinct types of SQR, four have representatives that have been biochemically characterized. The genome of Chlorobaculum tepidum encodes three SQR homologs. One of these, encoded by CT1087, is a type VI SQR that has been previously shown to be required for growth at high sulfide concentrations and to be expressed in sulfide-dependent manner. Therefore, CT1087 was hypothesized to be a high sulfide adapted SQR. CT1087 was expressed in Escherichia coli with an N-terminal His-tag (CT1087NHis6) and purified by Ni-NTA chromatography. CT1087NHis6 was active and contained FAD as a strongly bound cofactor. The measured kinetic parameters for CT1087NHis6 indicate a low affinity for sulfide and a high enzymatic turnover rate consistent with the hypothesis for its function inferred from genetic and expression data. These are the first kinetic data for a type VI SQR and have implications for structure-function analyses of all SQR's.
[Mh] Termos MeSH primário: Chlorobi/enzimologia
NAD(P)H Desidrogenase (Quinona)/genética
NAD(P)H Desidrogenase (Quinona)/metabolismo
Sulfetos/metabolismo
[Mh] Termos MeSH secundário: Chlorobi/crescimento & desenvolvimento
Chlorobi/metabolismo
Metabolismo Energético
Escherichia coli/genética
Flavina-Adenina Dinucleotídeo/metabolismo
Cinética
NAD(P)H Desidrogenase (Quinona)/isolamento & purificação
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Sulfides); 146-14-5 (Flavin-Adenine Dinucleotide); EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160519
[St] Status:MEDLINE



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