Base de dados : MEDLINE
Pesquisa : B03.275 [Categoria DeCS]
Referências encontradas : 516 [refinar]
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[PMID]:29364600
[Au] Autor:Chernitsyna SM; Khal'zov IA; Khanaeva TA; Morozov IV; Klimenkov IV; Pimenov NV; Zemskayal TI
[Ti] Título:Microbial Community Associated with Thioploca sp. Sheaths in the Area of the Posolski Bank Methane Seep, Southern Baikal.
[So] Source:Mikrobiologiia;85(5):522-530, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Bacterial mats formed by a colorless sulfur bacterium Thioploca sp. in the area of the Posolski Bank cold methane seep (southern Baikal) were -studied using electron microscopy and phylogenetic analysis. Morphologically the bacteria were identified as Thioploca ingrica.- Confocal microscopy of DAPI-stained samples revealed numerous rod-shaped, filamentous, and spiral microorganisms in the sheaths, as well as in- side and between the trichomes. Transmission electron microscopy revealed nonvacuolated bacteria and small cells-without cell envelopes within the sheath. Bacteria with pronounced intracytoplasmic membranes characteristic; of type I methanotrophs were observed at the outer side of the sheath. Based on analysis of the 16S rRNA gene sequences, the following phyla were idenified in the sheath community: Bacteroidetes, Nitro- spira, Chloroflexi, Planctomycetes, Verrucomicrobia,'y-, and 6-Proteobacteria, Euryarchaeota, Crenarchaeota, and Thaumarchaeota, as well as anammox bacteria. A hypothetical scheme of matter flows in the Lake Baikal bacterial mats was proposed based on the data on metabolism of the cultured homologues.
[Mh] Termos MeSH primário: Água Subterrânea/microbiologia
Lagos/microbiologia
Consórcios Microbianos/fisiologia
RNA Ribossômico 16S/genética
Thiotrichaceae/genética
[Mh] Termos MeSH secundário: Bacteroidetes/classificação
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Chloroflexi/classificação
Chloroflexi/genética
Chloroflexi/isolamento & purificação
Crenarchaeota/classificação
Crenarchaeota/genética
Crenarchaeota/isolamento & purificação
Ecossistema
Euryarchaeota/classificação
Euryarchaeota/genética
Euryarchaeota/isolamento & purificação
Metano/química
Metano/metabolismo
Filogenia
Planctomycetales/classificação
Planctomycetales/genética
Planctomycetales/isolamento & purificação
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/isolamento & purificação
Sibéria
Thiotrichaceae/classificação
Thiotrichaceae/isolamento & purificação
Thiotrichaceae/ultraestrutura
Verrucomicrobia/classificação
Verrucomicrobia/genética
Verrucomicrobia/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); OP0UW79H66 (Methane)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:28943350
[Au] Autor:Chen J; Wang PF; Wang C; Liu JJ; Gao H; Wang X
[Ad] Endereço:Key Laboratory of Integrated Regulation and Resource Department on Shallow Lakes, Ministry of Education, Hohai University, 1 Xikang Road, Nanjing 210098, PR China; College of Environment, Hohai University, 1 Xikang Road, Nanjing 210098, PR China; The State Key Laboratory of Lake Science and Environm
[Ti] Título:Spatial distribution and diversity of organohalide-respiring bacteria and their relationships with polybrominated diphenyl ether concentration in Taihu Lake sediments.
[So] Source:Environ Pollut;232:200-211, 2018 Jan.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:It is acknowledged that organohalide-respiring bacteria (OHRB) can degrade polybrominated diphenyl ethers (PBDEs); however, very little is known about the distribution of OHRB or their response to PBDE contamination in natural sediments. We collected sediments from 28 sampling sites in Taihu Lake, China, and investigated the spatial distribution and diversity of OHRB, and the relationships between the PBDE contamination levels and the PBDE removal potential. The abundances of five typical OHRB genera, namely Dehalobacter, Dehalococcoides, Dehalogenimonas, Desulfitobacterium, and Geobacter, ranged from 0.34 × 10 to 19.4 × 10 gene copies g dry sediment, and varied significantly among different areas of Taihu Lake. OHRB were more abundant in sediments from Meiliang and Zhushan Bay, where the PBDE concentrations were higher, and the phylotype diversity of the OHRB belonging to the family Dehalococcoidaceae was lower, than reported for other areas. While the sulfate concentrations explained much of the spatial distribution of OHRB, PBDE concentrations were also a strong influence on the abundance and diversity of OHRB in the sediments. For Dehalococcoides, Dehalogenimonas and Geobacter, the abundance of each genus was positively related to its own potential to remove PBDEs. The dominant OHRB genus, Dehalogenimonas, may contribute most to in situ bioremediation of PBDEs in Taihu Lake.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Monitoramento Ambiental
Éteres Difenil Halogenados/análise
Lagos/microbiologia
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Bactérias
Respiração Celular
China
Chloroflexi/genética
Desulfitobacterium/genética
Sedimentos Geológicos
Éteres Difenil Halogenados/metabolismo
Lagos/química
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Halogenated Diphenyl Ethers); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE


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[PMID]:28758628
[Au] Autor:Yabe S; Sakai Y; Abe K; Yokota A; Také A; Matsumoto A; Sugiharto A; Susilowati D; Hamada M; Nara K; Made Sudiana I; Otsuka S
[Ad] Endereço:1​Graduate School of Agricultural Sciences, Tohoku University, 468-1, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-0845, Japan 2​Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd., 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
[Ti] Título:Dictyobacter aurantiacus gen. nov., sp. nov., a member of the family Ktedonobacteraceae, isolated from soil, and emended description of the genus Thermosporothrix.
[So] Source:Int J Syst Evol Microbiol;67(8):2615-2621, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A mesophilic, Gram-stain-positive, spore-forming bacterium that formed branched mycelia was isolated from paddy soil in Gunung Salak (Mount Salak), West Java, Indonesia. This strain, designated S-27T, grew at temperatures between 20 and 37 °C; the optimum growth temperature was 25 to 30 °C, and no growth was observed at 15 or 45 °C. The pH range for growth was pH 3.5 to 8.6; the optimum pH was 6.0, and no growth was observed at pH 3.0 or 9.2. Strain S-27T was able to hydrolyse polysaccharides such as starch, cellulose and xylan. The G+C content of the DNA of strain S-27T was 55.7 mol%. The major fatty acids were iso-C17 : 0 and C16 : 1 2-OH, and the major menaquinone was MK-9 (H2). The cell wall of strain S-27T contained d-glutamic acid, glycine, l-alanine, d-alanine, l-ornithine and ß-alanine in a molar ratio of 1.0 : 1.6 : 1.4 : 0.6 : 0.9 : 1.1. The polar lipids consisted of phosphatidylglycerol, phosphatidylinositol and two glycolipids. The major cell-wall sugar was arabinose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S-27T belongs to the order Ktedonobacterales and is most closely related to Ktedonobacter racemifer SOSP1-21T (89.6 % sequence identity). On the basis of its chemotaxonomic and phenotypic features and phylogenetic position, we concluded that strain S-27T represents a novel genus and species, for which we propose the name Dictyobacter aurantiacus gen. nov., sp. nov. The type strain of Dictyobacter aurantiacus is strain S-27T (=NBRC 109595T=InaCC B312T). Emendation of the description of the genus Thermosporothrix is also provided.
[Mh] Termos MeSH primário: Chloroflexi/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Chloroflexi/genética
Chloroflexi/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Indonésia
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-39-7 (menaquinone 9)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001985


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[PMID]:28672697
[Au] Autor:Nemecek J; Dolinová I; Machácková J; Spánek R; Sevcu A; Lederer T; Cerník M
[Ad] Endereço:Technical University of Liberec, Studentská 2, CZ-461 17, Liberec, Czech Republic; ENACON s.r.o., Krcská 16, CZ-140 00, Prague 4, Czech Republic. Electronic address: jan.nemecek1@tul.cz.
[Ti] Título:Stratification of chlorinated ethenes natural attenuation in an alluvial aquifer assessed by hydrochemical and biomolecular tools.
[So] Source:Chemosphere;184:1157-1167, 2017 Oct.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biomolecular and hydrochemical tools were used to evaluate natural attenuation of chlorinated ethenes in a Quaternary alluvial aquifer located close to a historical source of large-scale tetrachloroethylene (PCE) contamination. Distinct stratification of redox zones was observed, despite the aquifer's small thickness (2.8 m). The uppermost zone of the target aquifer was characterised by oxygen- and nitrate-reducing conditions, with mixed iron- to sulphate-reducing conditions dominant in the lower zone, along with indications of methanogenesis. Natural attenuation of PCE was strongly influenced by redox heterogeneity, while higher levels of PCE degradation coincided with iron- to sulphate reducing conditions. Next generation sequencing of the middle and/or lower zones identified anaerobic bacteria (Firmicutes, Chloroflexi, Actinobacteria and Bacteroidetes) associated with reductive dechlorination. The relative abundance of dechlorinators (Dehalococcoides mccartyi, Dehalobacter sp.) identified by real-time PCR in soil from the lower levels supports the hypothesis that there is a significant potential for reductive dechlorination of PCE. Local conditions were insufficiently reducing for rapid complete dechlorination of PCE to harmless ethene. For reliable assessment of natural attenuation, or when designing monitoring or remedial systems, vertical stratification of key biological and hydrochemical markers should be analysed as standard, even in shallow aquifers.
[Mh] Termos MeSH primário: Monitoramento Ambiental
Etilenos/análise
Água Subterrânea/química
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Chloroflexi
Halogenação
Sequenciamento de Nucleotídeos em Larga Escala
Ferro/análise
Tetracloroetileno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenes); 0 (Water Pollutants, Chemical); 91GW059KN7 (ethylene); E1UOL152H7 (Iron); TJ904HH8SN (Tetrachloroethylene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE


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[PMID]:28654328
[Au] Autor:Fincker M; Spormann AM
[Ad] Endereço:Department of Civil and Environmental Engineering and Department of Chemical Engineering, Stanford University, Stanford, California 94305; email: mfincker@stanford.edu , spormann@stanford.edu.
[Ti] Título:Biochemistry of Catabolic Reductive Dehalogenation.
[So] Source:Annu Rev Biochem;86:357-386, 2017 Jun 20.
[Is] ISSN:1545-4509
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A wide range of phylogenetically diverse microorganisms couple the reductive dehalogenation of organohalides to energy conservation. Key enzymes of such anaerobic catabolic pathways are corrinoid and Fe-S cluster-containing, membrane-associated reductive dehalogenases. These enzymes catalyze the reductive elimination of a halide and constitute the terminal reductases of a short electron transfer chain. Enzymatic and physiological studies revealed the existence of quinone-dependent and quinone-independent reductive dehalogenases that are distinguishable at the amino acid sequence level, implying different modes of energy conservation in the respective microorganisms. In this review, we summarize current knowledge about catabolic reductive dehalogenases and the electron transfer chain they are part of. We review reaction mechanisms and the role of the corrinoid and Fe-S cluster cofactors and discuss physiological implications.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Chloroflexi/enzimologia
Coenzimas/química
Corrinoides/química
Halogênios/química
Oxirredutases/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Benzoquinonas/química
Benzoquinonas/metabolismo
Biocatálise
Chloroflexi/química
Chloroflexi/genética
Coenzimas/metabolismo
Corrinoides/metabolismo
Transporte de Elétrons
Metabolismo Energético
Expressão Gênica
Halogênios/metabolismo
Cinética
Modelos Moleculares
Oxirredutases/genética
Oxirredutases/metabolismo
Filogenia
Especificidade por Substrato
Vitamina B 12/química
Vitamina B 12/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Benzoquinones); 0 (Coenzymes); 0 (Corrinoids); 0 (Halogens); 3T006GV98U (quinone); EC 1.- (Oxidoreductases); P6YC3EG204 (Vitamin B 12)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170704
[Lr] Data última revisão:
170704
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE
[do] DOI:10.1146/annurev-biochem-061516-044829


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[PMID]:28531262
[Au] Autor:Liu Y; Vick-Majors TJ; Priscu JC; Yao T; Kang S; Liu K; Cong Z; Xiong J; Li Y
[Ad] Endereço:Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
[Ti] Título:Biogeography of cryoconite bacterial communities on glaciers of the Tibetan Plateau.
[So] Source:FEMS Microbiol Ecol;93(6), 2017 Jun 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cryoconite holes, water-filled pockets containing biological and mineralogical deposits that form on glacier surfaces, play important roles in glacier mass balance, glacial geochemistry and carbon cycling. The presence of cryoconite material decreases surface albedo and accelerates glacier mass loss, a problem of particular importance in the rapidly melting Tibetan Plateau. No studies have addressed the microbial community composition of cryoconite holes and their associated ecosystem processes on Tibetan glaciers. To further enhance our understanding of these glacial ecosystems on the Tibetan Plateau and to examine their role in carbon cycling as the glaciers respond to climate change, we explored the bacterial communities within cryoconite holes associated with three climatically distinct Tibetan Plateau glaciers using Illumina sequencing of the V4 region of the 16S rRNA gene. Cryoconite bacterial communities were dominated by Cyanobacteria, Chloroflexi, Betaproteobacteria, Bacteroidetes and Actinobacteria. Cryoconite bacterial community composition varied according to their geographical locations, exhibiting significant differences among glaciers studied. Regional beta diversity was driven by the interaction between geographic distance and environmental variables; the latter contributed more than geographic distance to the variation in cryoconite microbial communities. Our study is the first to describe the regional-scale spatial variability and to identify the factors that drive regional variability of cryoconite bacterial communities on the Tibetan Plateau.
[Mh] Termos MeSH primário: Actinobacteria/genética
Bacteroidetes/genética
Betaproteobacteria/genética
Chloroflexi/genética
Cianobactérias/genética
Camada de Gelo/microbiologia
[Mh] Termos MeSH secundário: Actinobacteria/isolamento & purificação
Bacteroidetes/isolamento & purificação
Betaproteobacteria/isolamento & purificação
Chloroflexi/isolamento & purificação
Mudança Climática
Cianobactérias/isolamento & purificação
Ecossistema
Geografia
Sequenciamento de Nucleotídeos em Larga Escala
Consórcios Microbianos/genética
RNA Ribossômico 16S/genética
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix072


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[PMID]:28431045
[Au] Autor:Zhong Q; Chen H; Liu L; He Y; Zhu D; Jiang L; Zhan W; Hu J
[Ad] Endereço:Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
[Ti] Título:Water table drawdown shapes the depth-dependent variations in prokaryotic diversity and structure in Zoige peatlands.
[So] Source:FEMS Microbiol Ecol;93(6), 2017 Jun 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microbial communities are important to ecosystem function and sensitive to hydrological dynamics. However, we lack predictable knowledge about how soil microorganisms respond to water table drawdown in different depths. This research used a high-throughput sequencing method to determine the responses of prokaryotic communities to the changes of water table and depth on Zoige peatlands. Our results showed that water table drawdown reduced alpha diversity indices (observed species, Shannon diversity and Chao1 richness) of prokaryotic communities. Intriguingly, the reduction of diversity varied in different depths, and was statistically significant in intermediate layers (20-30 cm and 50-60 cm), but not in the surface (0-10 cm) or deep layer (90-100 cm). In deeper layers there was greater relative abundance of most anaerobic microorganisms (e.g. Chloroflexi, Planctomyctes and NC10), but lesser amounts of most aerobes (e.g. Proteobacteria and Acidobacteria). However, the vertical distribution of prokaryotic microbiota along the depth gradient was altered by water table drawdown, mainly by enriching oligotrophs (e.g. Acidobcteria) over copiotrophs (e.g. Bacteriodetes). In addition, we found that the most important soil parameters influencing community structure were soil pH, total organic carbon and total nitrogen. Our study illuminates that the variations of prokaryotic communities caused by water table drawdown are depth-dependent, and that water table drawdown leads to predictive changes of microbiota in peatlands.
[Mh] Termos MeSH primário: Acidobacteria/crescimento & desenvolvimento
Chloroflexi/crescimento & desenvolvimento
Água Subterrânea/microbiologia
Planctomycetales/crescimento & desenvolvimento
Solo/química
[Mh] Termos MeSH secundário: Acidobacteria/classificação
Acidobacteria/genética
Biodiversidade
Chloroflexi/classificação
Chloroflexi/genética
Ecossistema
Sequenciamento de Nucleotídeos em Larga Escala
Nitrogênio
Planctomycetales/classificação
Planctomycetales/genética
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); N762921K75 (Nitrogen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix049


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[PMID]:28371548
[Au] Autor:Styles MQ; Nesbitt EA; Marr S; Hutchby M; Leak DJ
[Ad] Endereço:Department of Biology and Biochemistry, University of Bath, UK.
[Ti] Título:Characterization of the first naturally thermostable terpene synthases and development of strategies to improve thermostability in this family of enzymes.
[So] Source:FEBS J;284(11):1700-1711, 2017 Jun.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The terpenoid family of natural products is being targeted for heterologous microbial production as a cheaper and more reliable alternative to extraction from plants. The key enzyme responsible for diversification of terpene structure is the class-I terpene synthase (TS), and these often require engineering to improve properties such as thermostability, robustness and catalytic activity before they are suitable for industrial use. Improving thermostability typically relies on screening a large number of mutants, as there are no naturally thermostable TSs described upon which to base rational design decisions. We have characterized the first examples of natural TSs exhibiting thermostability, which catalyse the formation of the sesquiterpene τ-muurolol at temperatures up to 78 °C. We also report an enzyme with a k value of 0.95 s at 65 °C, the highest k recorded for a bacterial sesquiterpene synthase. In turn, these thermostable enzymes were used as a model to inform the rational engineering of another TS, with the same specificity but low sequence identity to the model. The newly engineered variant displayed increased thermostability and turnover. Given the high structural homology of the class-I TS domain, this approach could be generally applicable to improving the properties of other enzymes in this class. DATABASE: Model data are available in the PMDB database under the accession number PM0080780.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/química
Chloroflexi/enzimologia
[Mh] Termos MeSH secundário: Escherichia coli
Temperatura Alta
Cinética
Modelos Químicos
Modelos Moleculares
Fosfatos de Poli-Isoprenil/metabolismo
Conformação Proteica
Estabilidade Proteica
Proteínas Recombinantes/metabolismo
Sesquiterpenos/metabolismo
Relação Estrutura-Atividade
Terpenos/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polyisoprenyl Phosphates); 0 (Recombinant Proteins); 0 (Sesquiterpenes); 0 (Terpenes); 79W6B01D07 (farnesyl pyrophosphate); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.- (terpene synthase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1111/febs.14072


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[PMID]:28343057
[Au] Autor:Cheng C; Zhou Z; Niu T; An Y; Shen X; Pan W; Chen Z; Liu J
[Ad] Endereço:College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai 200090, China.
[Ti] Título:Effects of side-stream ratio on sludge reduction and microbial structures of anaerobic side-stream reactor coupled membrane bioreactors.
[So] Source:Bioresour Technol;234:380-388, 2017 Jun.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An anoxic/oxic membrane bioreactor (AO-MBR) and three anaerobic side-stream reactor (ASSR) coupled MBRs (ASSR-MBR) were operated to investigate effects of side-stream ratio (SR) on sludge reduction and microbial community structure of ASSR-MBRs. The ASSR-MBR achieved efficient COD and ammonium nitrogen removal. SR increased from 0.2 to 1.0 favored nitrogen removal, and increased sludge reduction from 6.0% to 49.7%. The total released COD in the ASSR increased with the rising SR and was inversely proportional to sludge yield of ASSR-MBR. Pyrosequencing analysis showed that phyla Chloroflexi and Armatimonadetes surviving in anaerobic conditions were enriched in the ASSR, while Nitrospirae was dominant in the MBR. Comparison at the genus level revealed that higher SR favored the growth of slow growers, while lower SR enriched hydrolytic and predatory bacteria. The results suggested that SR has a profound effect on nitrogen removal, sludge reduction and microbial community structure in the ASSR-MBR.
[Mh] Termos MeSH primário: Reatores Biológicos/microbiologia
Esgotos/análise
[Mh] Termos MeSH secundário: Compostos de Amônio/isolamento & purificação
Anaerobiose
Biodegradação Ambiental
Análise da Demanda Biológica de Oxigênio
Chloroflexi/crescimento & desenvolvimento
Hidrólise
Oxirredução
Esgotos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Sewage)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170327
[St] Status:MEDLINE


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[PMID]:28321007
[Au] Autor:Yabe S; Sakai Y; Abe K; Yokota A
[Ad] Endereço:Graduate School of Agricultural Sciences, Tohoku University.
[Ti] Título:Diversity of Ktedonobacteria with Actinomycetes-Like Morphology in Terrestrial Environments.
[So] Source:Microbes Environ;32(1):61-70, 2017 Mar 31.
[Is] ISSN:1347-4405
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Bacteria with an actinomycetes-like morphology have recently been discovered, and the class Ktedonobacteria was created for these bacteria in the phylum Chloroflexi. They may prove to be a valuable resource with the potential to produce unprecedented secondary metabolites. However, our understanding of their diversity, richness, habitat, and ecological significance is very limited. We herein developed a 16S rRNA gene-targeted, Ktedonobacteria-specific primer and analyzed ktedonobacterial amplicons. We investigated abundance, diversity, and community structure in forest and garden soils, sand, bark, geothermal sediment, and compost. Forest soils had the highest diversity among the samples tested (1181-2934 operational taxonomic units [OTUs]; Chao 1 estimate, 2503-5613; Shannon index, 4.21-6.42). A phylogenetic analysis of representative OTUs revealed at least eight groups within unclassified Ktedonobacterales, expanding the known diversity of this order. Ktedonobacterial communities markedly varied among our samples. The common mesic environments (soil, sand, and bark) were dominated by diverse phylotypes within the eight groups. In contrast, compost and geothermal sediment samples were dominated by known ktedonobacterial families (Thermosporotrichaceae and Thermogemmatisporaceae, respectively). The relative abundance of Ktedonobacteria in the communities, based on universal primers, was ≤0.8%, but was 12.9% in the geothermal sediment. These results suggest that unknown diverse Ktedonobacteria inhabit common environments including forests, gardens, and sand at low abundances, as well as extreme environments such as geothermal areas.
[Mh] Termos MeSH primário: Biodiversidade
Chloroflexi/classificação
Chloroflexi/genética
Microbiologia Ambiental
[Mh] Termos MeSH secundário: Chloroflexi/citologia
Análise por Conglomerados
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1264/jsme2.ME16144



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