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[PMID]:29458469
[Au] Autor:Cai H; Cui H; Zeng Y; An M; Jiang H
[Ad] Endereço:1​State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, PR China.
[Ti] Título:Sandarakinorhabdus cyanobacteriorum sp. nov., a novel bacterium isolated from cyanobacterial aggregates in a eutrophic lake.
[So] Source:Int J Syst Evol Microbiol;68(3):730-735, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bacterial strain, designated TH057 , was isolated from cyanobacterial aggregates in a eutrophic lake in China. Cells were observed to be slightly curved, rod-shaped, capsule-forming and stained Gram-negative. Optimal growth was obtained at pH 7.0 (range: pH 5-9) and 30 °C (range: 20-37 °C) in R2A broth. According to the absorption spectrum, carotenoids (455 and 490 nm) and light-harvesting complex LHI (857 nm) were present in the cells. The cells were found to be positive for oxidase and catalase activities. The major respiratory quinone was ubiquinone Q-10. The major fatty acids were identified as C17 : 1ω6c, C16 : 1ω7c/C16 : 1ω6c, C18 : 1ω6c/C18 : 1ω7c and C16 : 0. The major polar lipids were found to consist of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified glycolipid and two sphingoglycolipids. Strain TH057 shared highest 16S rRNA gene sequence similarity to Sandarakinorhabdus limnophila so42 (96.8 %), followed by Polymorphobacter fuscus D40P (95.8 %). The genomic G+C content of strain TH057 was 66.1 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization value for the complete genomes were 81.0 and 23.0 % between strain TH057 and Sandarakinorhabdus limnophila so42 . The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain TH057 represents a novel species within the genus Sandarakinorhabdus, for which the name Sandarakinorhabduscyanobacteriorum sp. nov. is proposed. The type strain is TH057 (=CGMCC 1.15803 =LMG 30294 ).
[Mh] Termos MeSH primário: Lagos/microbiologia
Filogenia
Sphingomonadaceae/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
Cianobactérias
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Sphingomonadaceae/genética
Sphingomonadaceae/isolamento & purificação
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002571


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[PMID]:29358154
[Au] Autor:Turner AD; Waack J; Lewis A; Edwards C; Lawton L
[Ad] Endereço:Centre for Environment, Fisheries and Aquaculture Science, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom. Electronic address: andrew.turner@cefas.co.uk.
[Ti] Título:Development and single-laboratory validation of a UHPLC-MS/MS method for quantitation of microcystins and nodularin in natural water, cyanobacteria, shellfish and algal supplement tablet powders.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1074-1075:111-123, 2018 Feb 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple, rapid UHPLC-MS/MS method has been developed and optimised for the quantitation of microcystins and nodularin in wide variety of sample matrices. Microcystin analogues targeted were MC-LR, MC-RR, MC-LA, MC-LY, MC-LF, LC-LW, MC-YR, MC-WR, [Asp3] MC-LR, [Dha7] MC-LR, MC-HilR and MC-HtyR. Optimisation studies were conducted to develop a simple, quick and efficient extraction protocol without the need for complex pre-analysis concentration procedures, together with a rapid sub 5min chromatographic separation of toxins in shellfish and algal supplement tablet powders, as well as water and cyanobacterial bloom samples. Validation studies were undertaken on each matrix-analyte combination to the full method performance characteristics following international guidelines. The method was found to be specific and linear over the full calibration range. Method sensitivity in terms of limits of detection, quantitation and reporting were found to be significantly improved in comparison to LC-UV methods and applicable to the analysis of each of the four matrices. Overall, acceptable recoveries were determined for each of the matrices studied, with associated precision and within-laboratory reproducibility well within expected guidance limits. Results from the formalised ruggedness analysis of all available cyanotoxins, showed that the method was robust for all parameters investigated. The results presented here show that the optimised LC-MS/MS method for cyanotoxins is fit for the purpose of detection and quantitation of a range of microcystins and nodularin in shellfish, algal supplement tablet powder, water and cyanobacteria. The method provides a valuable early warning tool for the rapid, routine extraction and analysis of natural waters, cyanobacterial blooms, algal powders, food supplements and shellfish tissues, enabling monitoring labs to supplement traditional microscopy techniques and report toxicity results within a short timeframe of sample receipt. The new method, now accredited to ISO17025 standard, is simple, quick, applicable to multiple matrices and is highly suitable for use as a routine, high-throughout, fast turnaround regulatory monitoring tool.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Cianobactérias/química
Suplementos Nutricionais/análise
Microcistinas/análise
Peptídeos Cíclicos/análise
Frutos do Mar/análise
[Mh] Termos MeSH secundário: Animais
Produtos Biológicos/análise
Produtos Biológicos/química
Bivalves
Contaminação de Alimentos
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
Comprimidos
Espectrometria de Massas em Tandem/métodos
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Microcystins); 0 (Peptides, Cyclic); 0 (Tablets); 0 (Water Pollutants, Chemical); 0979BIK2QU (nodularin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE


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[PMID]:28968514
[Au] Autor:Huang Y; Li P; Chen G; Peng L; Chen X
[Ad] Endereço:Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, No.500 Dong Chuan Road, Shanghai 200241, PR China.
[Ti] Título:The production of cyanobacterial carbon under nitrogen-limited cultivation and its potential for nitrate removal.
[So] Source:Chemosphere;190:1-8, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Harmful cyanobacterial blooms (CyanoHABs) represent a serious threat to aquatic ecosystems. A beneficial use for these harmful microorganisms would be a promising resolution of this urgent issue. This study applied a simple method, nitrogen limitation, to cultivate cyanobacteria aimed at producing cyanobacterial carbon for denitrification. Under nitrogen-limited conditions, the common cyanobacterium, Microcystis, efficiently used nitrate, and had a higher intracellular C/N ratio. More importantly, organic carbons easily leached from its dry powder; these leachates were biodegradable and contained a larger amount of dissolved organic carbon (DOC) and carbohydrates, but a smaller amount of dissolved total nitrogen (DTN) and proteins. When applied to an anoxic system with a sediment-water interface, a significant increase of the specific NO -N removal rate was observed that was 14.2 times greater than that of the control. This study first suggests that nitrogen-limited cultivation is an efficient way to induce organic and carbohydrate accumulation in cyanobacteria, as well as a high C/N ratio, and that these cyanobacteria can act as a promising carbon source for denitrification. The results indicate that application as a carbon source is not only a new way to utilize cyanobacteria, but it also contributes to nitrogen removal in aquatic ecosystems, further limiting the proliferation of CyanoHABs.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Carbono/metabolismo
Cianobactérias/metabolismo
Desnitrificação
Nitratos/isolamento & purificação
Nitrogênio/deficiência
[Mh] Termos MeSH secundário: Ecossistema
Microcystis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrates); 7440-44-0 (Carbon); N762921K75 (Nitrogen)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


  4 / 12762 MEDLINE  
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[PMID]:28595450
[Au] Autor:Jiang W; Zhou W; Othman R; Uchida H; Watanabe R; Suzuki T; Sakamoto B; Nagai H
[Ad] Endereço:a Department of Ocean Sciences , Tokyo University of Marine Science and Technology , Tokyo , Japan.
[Ti] Título:A new malyngamide from the marine cyanobacterium Moorea producens.
[So] Source:Nat Prod Res;32(1):97-104, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new malyngamide (1) was isolated along with seven known compounds (2-8) from the marine cyanobacterium Moorea producens collected in Hawaii. Compound 1 represented the first reported malyngamide with a hydroxy moiety at C-7 of the characteristic fatty acid portion of the compound. Compound 1 showed cytotoxicity against L1210 cell line at an IC value of 2.9 mM and lethal toxicity against the shrimp Palaemon paucidens at a LD value of 33.3 mg/kg. The bioactivity of compound 1 was approximately 10-100 times weaker than those of isomalyngamides A and B (3, 4). These results indicated that the methoxy group at C-7 of the fatty acid section confers a degree of bioactivity in malyngamides.
[Mh] Termos MeSH primário: Cianobactérias/química
Lipopeptídeos/química
Lipopeptídeos/farmacologia
[Mh] Termos MeSH secundário: Amidas/química
Amidas/farmacologia
Animais
Organismos Aquáticos/química
Linhagem Celular Tumoral
Leucemia L1210
Espectroscopia de Ressonância Magnética
Camundongos
Estrutura Molecular
Palaemonidae/efeitos dos fármacos
Pirróis/química
Pirróis/farmacologia
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amides); 0 (Lipopeptides); 0 (Pyrroles); 0 (isomalyngamide A); 0 (isomalyngamide B)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1338282


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[PMID]:29406099
[Au] Autor:Lin JL; Hua LC; Hung SK; Huang C
[Ad] Endereço:Department of Environmental Engineering, Chung Yuan Christian University, Taoyuan, Chinese Taipei. Electronic address: jrlin@cycu.edu.tw.
[Ti] Título:Algal removal from cyanobacteria-rich waters by preoxidation-assisted coagulation-flotation: Effect of algogenic organic matter release on algal removal and trihalomethane formation.
[So] Source:J Environ Sci (China);63:147-155, 2018 Jan.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The cyanobacteria-bloom in raw waters frequently causes an unpredictable chemical dosing of preoxidation and coagulation for an effective removal of algal cells in water treatment plants. This study investigated the effects of preoxidation with NaOCl and ClO on the coagulation-flotation effectiveness in the removal of two commonly blooming cyanobacteria species, Microcystis aeruginosa (MA) and Cylindrospermopsis raciborskii (CR), and their corresponding trihalomethane (THM) formation potential. The results showed that dual dosing with NaOCl plus ClO was more effective in enhancing the deformation of cyanobacterial cells compared to single dosing with NaOCl, especially for CR-rich water. Both preoxidation approaches for CR-rich water effectively reduced the CR cell count with less remained dissolved organic carbon (DOC), which benefited subsequent coagulation-flotation. However, preoxidation led to an adverse release of algogenic organic matter (AOM) in the case of MA-rich water. The release of AOM resulted in a poor removal in MA cells and a large amount of THM formation after oxidation-assisted coagulation-flotation process. The reduction in THM formation potential of CR-rich waters is responsible for effective algae and DOC removal by alum coagulation. It is concluded that the species-specific characteristic of cyanobacteria and their AOM released during chlorination significantly influences the performance of coagulation-flotation for AOM removal and corresponding THM formation.
[Mh] Termos MeSH primário: Cianobactérias/fisiologia
Microcystis/fisiologia
Trialometanos/metabolismo
Eliminação de Resíduos Líquidos/métodos
[Mh] Termos MeSH secundário: Microalgas/fisiologia
Oxirredução
Trialometanos/análise
Trialometanos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trihalomethanes)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  6 / 12762 MEDLINE  
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[PMID]:29239717
[Au] Autor:Cai H; Cui H; Zeng Y; Wang Y; Jiang H
[Ad] Endereço:1​State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, PR China.
[Ti] Título:Niveispirillum lacus sp. nov., isolated from cyanobacterial aggregates in a eutrophic lake.
[So] Source:Int J Syst Evol Microbiol;68(2):507-512, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bacterial strain, 1-14 , was isolated from cyanobacterial aggregates in a eutrophic lake, Taihu Lake, China. Cells were observed to be slightly curved, rod-shaped, aerobic and Gram-stain-negative. Optimal growth occurred at pH 7.0 (range: 5.0-9.0), 28 °C (range: 20-32 °C) and 0 % (w/v) NaCl (range: 0-1.0 %) in R2A broth. No growth is observed at 37 °C. The cells were found to be positive for oxidase and catalase activities. The major respiratory quinone was ubiquinone Q-10. The major fatty acids (>10 %) were identified as C18 : 1ω6c/C18 : 1ω7c, C16 : 0 3-OH and C18 : 1 2-OH. The major polar lipids were found to consist of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine. Within the genus Niveispirillum, strain 1-14 was most closely related to Niveispirillum cyanobacteriorum TH16 (98.3 % 16S rRNA gene sequence similarity), followed by Niveispirillum irakense DSM 11586 (97.8 %) and Niveispirillum fermenti CC-LY736 (97.0 %). The genomic G+C content of strain 1-14 was 62.2 mol% based on total genome calculations. Genes coding for light-harvesting complexes LHI and LHII, and a photosynthetic reaction centre were detected in the genome. Average nucleotide identities and digital DNA-DNA hybridizations for complete genomes ranged from 76.4 to 83.5 and from 21.5 to 27.4 % between strain 1-14 and strains within the genus Niveispirillum. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain 1-14 represents a novel species within the genus Niveispirillum, for which the name Niveispirillum lacus sp. nov. is proposed. The type strain is 1-14 (=CGMCC 1.12980 =LMG 28363 ).
[Mh] Termos MeSH primário: Eutrofização
Lagos/microbiologia
Filogenia
Rhodospirillaceae/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
Cianobactérias/classificação
Cianobactérias/genética
Cianobactérias/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Rhodospirillaceae/genética
Rhodospirillaceae/isolamento & purificação
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002526


  7 / 12762 MEDLINE  
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[PMID]:29229390
[Au] Autor:Wang J; Chen CC; Yang Y; Liu W; Ko TP; Shang N; Hu X; Xie Y; Huang JW; Zhang Y; Guo RT
[Ad] Endereço:Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
[Ti] Título:Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis.
[So] Source:Biochem Biophys Res Commun;495(2):1782-1788, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:FamD1 is a novel CloQ/NphB-family indole prenyltransferase which involves in hapalindole-type alkaloid biosynthesis. Here the native FamD1 structure and three protein-ligand complexes are analyzed to investigate the molecular basis of substrate binding and catalysis. FamD1 adopts a typical ABBA architecture of aromatic prenyltransferase, in which the substrate-binding chamber is found in the central ß-barrel. The indole-containing acceptor substrate is bound adjacent to the prenyl donor. Based on the complex structures, a catalytic mechanism of FamD1 is proposed. Functional implications on the sister enzyme FamD2 are also discussed.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Dimetilaliltranstransferase/química
Dimetilaliltranstransferase/metabolismo
Alcaloides de Indol/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Domínio Catalítico
Cristalografia por Raios X
Cianobactérias/enzimologia
Cianobactérias/genética
Dimetilaliltranstransferase/genética
Alcaloides de Indol/química
Modelos Moleculares
Conformação Proteica
Dobramento de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Indole Alkaloids); 0 (Recombinant Proteins); EC 2.5.1.1 (Dimethylallyltranstransferase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


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[PMID]:27775357
[Au] Autor:Wang C; Zhao C; Hu L; Chen H
[Ad] Endereço:Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
[Ti] Título:Calculated Mechanism of Cyanobacterial Aldehyde-Deformylating Oxygenase: Asymmetric Aldehyde Activation by a Symmetric Diiron Cofactor.
[So] Source:J Phys Chem Lett;7(21):4427-4432, 2016 Nov 03.
[Is] ISSN:1948-7185
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyanobacterial aldehyde-deformylating oxygenase (cADO) is a nonheme diiron enzyme that catalyzes the conversion of aldehyde to alk(a/e)ne, an important transformation in biofuel research. In this work, we report a highly desired computational study for probing the mechanism of cADO. By combining our QM/MM results with the available Fe Mössbauer spectroscopic data, the gained detailed structural information suggests construction of asymmetry from the symmetric diiron cofactor in an aldehyde substrate and O activation. His , one of the two iron-coordinate histidine residues in cADO, plays a pivotal role in this asymmetric aldehyde activation process by unprecedented reversible dissociation from the diiron cofactor, a behavior unknown in any other nonheme dinuclear or mononuclear enzymes. The revealed intrinsically asymmetric interactions of the substrate/O with the symmetric cofactor in cADO are inspirational for exploring diiron subsite resolution in other nonheme diiron enzymes.
[Mh] Termos MeSH primário: Aldeídos/química
Cianobactérias/química
Oxigenases/química
[Mh] Termos MeSH secundário: Catálise
Oxirredução
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Aldehydes); EC 1.13.- (Oxygenases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE


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[PMID]:27771810
[Au] Autor:Conceição de Souza R; de Medeiros Muniz G; Siqueira AS; de Melo Lima A; da Silva AP; Gonçalves EC; da Silva Gonçalves Vianez Júnior JL
[Ad] Endereço:Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, PA, 67030-000, Brazil.
[Ti] Título:Investigating the effects of point mutations on the affinity between the cyanobacterial lectin microvirin and high mannose-type glycans present on the HIV envelope glycoprotein.
[So] Source:J Mol Model;22(11):269, 2016 Nov.
[Is] ISSN:0948-5023
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Human immunodeficiency virus (HIV) infections continue to exert an enormous impact on global human health. This led experts to emphasize the importance of new measures for preventing HIV infections, including the development of vaccines and novel drugs. In this context, a promising approach involves the use of lectins that can bind the surface envelope glycoprotein gp120 of HIV with high affinity, preventing viral entry. The cyanobacterial lectin microvirin (MVN) has been proposed as a candidate for development as a topical microbicide because of its ability to bind to high mannose-type glycans, potently inhibiting HIV-1 entry. Thus, the aim of this computational study was to investigate the effects of four point mutations (D53Q, D53E, D53K, and D53W) on the structure and affinity of MVN with di-mannose (MAN). Molecular dynamics simulations followed by binding free energy calculations using MM-GBSA were employed. The calculated binding free energy of ligand-receptor complexation of MVN with MAN was -26.02 kcal mol . We identified in the wild-type protein that residues I45, T59, and Q81 have a major contribution to the binding free energy of di-mannose. Among the investigated mutants, the most promising one was the D53W mutation, with a theoretical binding free energy value of -29.16 kcal mol . We suggest that this increased stability is due to the introduction of extra rigidity on the hinge region connecting two key structural elements of the MVN binding site.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Cianobactérias/química
Proteína gp120 do Envelope de HIV/química
HIV-1/química
Lectina de Ligação a Manose/química
Simulação de Dinâmica Molecular
Mutação Puntual
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Proteínas de Bactérias/genética
Sítios de Ligação
Proteína gp120 do Envelope de HIV/genética
HIV-1/genética
Lectina de Ligação a Manose/genética
Simulação de Acoplamento Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (HIV Envelope Protein gp120); 0 (Mannose-Binding Lectin); 0 (gp120 protein, Human immunodeficiency virus 1)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  10 / 12762 MEDLINE  
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[PMID]:29272288
[Au] Autor:Fukushima M; Tomioka N; Jutagate T; Hiroki M; Murata T; Preecha C; Avakul P; Phomikong P; Imai A
[Ad] Endereço:Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Tsukuba, Japan.
[Ti] Título:The dynamics of pico-sized and bloom-forming cyanobacteria in large water bodies in the Mekong River Basin.
[So] Source:PLoS One;12(12):e0189609, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the face of plans for increased construction of dams and reservoirs in the Mekong River Basin, it is critically important to better understand the primary-producer community of phytoplankton, especially the warm-water cyanobacteria. This is because these algae can serve as the primary source of carbon for higher trophic levels, including fishes, but can also form harmful blooms, threatening local fisheries and environmental and human health. We monitored the dynamics of three cyanobacteria-Synechococcus spp., Microcystis aeruginosa, and Dolichospermum spp.-for two years in nine large lakes and reservoirs in the Mekong River Basin. The densities of these algae were largely system-specific such that their abundance was uniquely determined within individual water bodies. However, after accounting for the system-specific effect, we found that cell densities of Synechococcus spp., M. aeruginosa, and Dolichospermum spp. varied in response to changes in photosynthetically active radiation (PAR), total nitrogen, and water level, respectively. Because both PAR and water level tend to fluctuate concordantly over a wide geographic area, Synechococcus spp., and to a lesser extent Dolichospermum spp., varied synchronously among the water bodies. Sustaining the production of pico-sized primary producers while preventing harmful algal blooms will be a key management goal for the proposed reservoirs in the Mekong Basin.
[Mh] Termos MeSH primário: Proliferação Nociva de Algas
Rios/microbiologia
[Mh] Termos MeSH secundário: Ásia Sudeste
Cianobactérias/classificação
Cianobactérias/genética
Cianobactérias/isolamento & purificação
Cianobactérias/fisiologia
DNA Bacteriano/isolamento & purificação
Fotossíntese
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171223
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189609



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