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Pesquisa : B03.280.110 [Categoria DeCS]
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  1 / 104 MEDLINE  
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[PMID]:28245621
[Au] Autor:Roy-Lachapelle A; Solliec M; Bouchard MF; Sauvé S
[Ad] Endereço:Department of Chemistry, Université de Montréal, Montréal, QC H3T 1J4, Canada. a.roy.lachapelle@umontreal.ca.
[Ti] Título:Detection of Cyanotoxins in Algae Dietary Supplements.
[So] Source:Toxins (Basel);9(3), 2017 Feb 25.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Algae dietary supplements are marketed worldwide as natural health products. Although their proprieties have been claimed as beneficial to improve overall health, there have been several previous reports of contamination by cyanotoxins. These products generally contain non-toxic cyanobacteria, but the methods of cultivation in natural waters without appropriate quality controls allow contamination by toxin producer species present in the natural environment. In this study, we investigated the presence of total microcystins, seven individual microcystins (RR, YR, LR, LA, LY, LW, LF), anatoxin-a, dihydroanatoxin-a, epoxyanatoxin-a, cylindrospermopsin, saxitoxin, and ß-methylamino-l-alanine in 18 different commercially available products containing or . Total microcystins analysis was accomplished using a Lemieux oxidation and a chemical derivatization using dansyl chloride was needed for the simultaneous analysis of cylindrospermopsin, saxitoxin, and ß-methylamino-l-alanine. Moreover, the use of laser diode thermal desorption (LDTD) and ultra-high performance liquid chromatography (UHPLC) both coupled to high resolution mass spectrometry (HRMS) enabled high performance detection and quantitation. Out of the 18 products analyzed, 8 contained some cyanotoxins at levels exceeding the tolerable daily intake values. The presence of cyanotoxins in these algal dietary supplements reinforces the need for a better quality control as well as consumer's awareness on the potential risks associated with the consumption of these supplements.
[Mh] Termos MeSH primário: Aphanizomenon
Toxinas Bacterianas/análise
Suplementos Nutricionais/análise
Spirulina
[Mh] Termos MeSH secundário: Adulto
Criança
Seres Humanos
Medição de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE


  2 / 104 MEDLINE  
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[PMID]:28235579
[Au] Autor:Chernova E; Sidelev S; Russkikh I; Voyakina E; Babanazarova O; Romanov R; Kotovshchikov A; Mazur-Marzec H
[Ad] Endereço:Saint-Petersburg Scientific Research Centre for Ecological Safety, Russian Academy of Sciences, Korpusnaya str., 18, Saint-Petersburg, 197110, Russia. Electronic address: s3561389@yandex.ru.
[Ti] Título:Dolichospermum and Aphanizomenon as neurotoxins producers in some Russian freshwaters.
[So] Source:Toxicon;130:47-55, 2017 May.
[Is] ISSN:1879-3150
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Last decades, cyanobacterial blooms have been commonly reported in Russia. Among the boom-forming species, potential toxin producers have been identified. The aim of this paper was to study the presence of neurotoxic compounds - saxitoxins and anatoxin-a - in water bodies from different regions of Russia. We also made attempts to identify the neurotoxin-producing genera. The good convergence of the results obtained by light microscopy, PCR and LC-MS/MS analyses indicated the presence of active neurotoxin producing species in all investigated water bodies. Saxitoxin was detected in phytoplankton from 4 water bodies in Central European Russia and West Siberia, including lake and reservoirs used as a source for potable water. The water bodies differed with the respect of saxitoxin producers which belonged to Aphanizomenon and/or Dolichospermum genera. For the first time, we obtained quantitative data on the intracellular saxitoxin concentration in Russian freshwaters using LC-MS/MS. Anatoxin-a was detected only in lakes of Northwestern Russia. In the eutrophic shallow Lower Suzdal Lake, Aphanizomenon was the stated anatoxin-a-producing genus. In the large shallow artificial hypertrophic Sestroretskij Razliv Lake, it was very likely that both dominant species - Aphanizomenon flos-aquae and Dolichospermum planctonicum - were anatoxin-a producers.
[Mh] Termos MeSH primário: Aphanizomenon/metabolismo
Cianobactérias/metabolismo
Água Doce/química
Neurotoxinas/metabolismo
[Mh] Termos MeSH secundário: Aphanizomenon/genética
Aphanizomenon/isolamento & purificação
Cromatografia Líquida
Cianobactérias/genética
Cianobactérias/isolamento & purificação
Monitoramento Ambiental
Água Doce/microbiologia
Espectrometria de Massas
Neurotoxinas/química
Neurotoxinas/isolamento & purificação
Federação Russa
Saxitoxina/química
Saxitoxina/isolamento & purificação
Saxitoxina/metabolismo
Tropanos/química
Tropanos/isolamento & purificação
Tropanos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurotoxins); 0 (Tropanes); 35523-89-8 (Saxitoxin); 80023A73NK (anatoxin a)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


  3 / 104 MEDLINE  
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[PMID]:27975199
[Au] Autor:Zhang W; Jeppesen E; Wang M; Xu X; Wang L
[Ad] Endereço:College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
[Ti] Título:Allelopathic effect boosts Chrysosporum ovalisporum dominance in summer at the expense of Microcystis panniformis in a shallow coastal water body.
[So] Source:Environ Sci Pollut Res Int;24(5):4666-4675, 2017 Feb.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The increased occurrence of harmful cyanobacterial species and, with this, higher frequency of cyanobacteria blooms, closely associated with eutrophication and climate change, have attracted increasing attention worldwide. However, competition mechanisms between the different bloom-forming cyanobacteria species remain to be elucidated. In this paper, for the first time, the allelopathic effect of the cyanobacterium Chrysosporum ovalisporum on the cyanobacterium Microcystis panniformis is reported. The results of our study conducted in a Chinese shallow coastal water body demonstrated that the biomass of M. panniformis was relatively low during the C. ovalisporum blooming period. Co-cultivation of a C. ovalisporum strain with a M. panniformis strain showed strong inhibition of the growth of M. panniformis but stimulation of C. ovalisporum. Thus, filtrate of C. ovalisporum culture had a strong inhibitory effect on the performance of M. panniformis by decreasing the maximum optical quantum yield (F /F ), the electron transport rate (ETR) of PS II and the onset of light saturation (I ) and by increasing the alkaline phosphatase (ALP) activity and superoxide dismutase (SOD) activity of M. panniformis. Our results suggest that the inter-specific allelopathic effect plays an important role in the competition between different cyanobacteria species. We foresee the importance of C. ovalisporum to intensify in a future warmer world, not least in small- to medium-sized, warm and high conductivity coastal water bodies.
[Mh] Termos MeSH primário: Alelopatia
Aphanizomenon/fisiologia
Microcystis/fisiologia
[Mh] Termos MeSH secundário: Biomassa
Oceanos e Mares
Estações do Ano
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-8149-0


  4 / 104 MEDLINE  
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[PMID]:26714798
[Au] Autor:Guzmán-Guillén R; Prieto Ortega AI; Moyano R; Blanco A; Vasconcelos V; Cameán AM
[Ad] Endereço:Area of Toxicology, Faculty of Pharmacy, University of Sevilla, C/Profesor García González 2, Sevilla, 41012, Spain.
[Ti] Título:Dietary l-carnitine prevents histopathological changes in tilapia (Oreochromis Niloticus) exposed to cylindrospermopsin.
[So] Source:Environ Toxicol;32(1):241-254, 2017 Jan.
[Is] ISSN:1522-7278
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cylindrospermopsin (CYN) is a cytotoxin highly water-soluble, which is easily taken up by several aquatic organisms. CYN acts as a potent protein and glutathione synthesis inhibitor, as well as inducing genotoxicity, oxidative stress, and histopathological alterations. This is the first study reporting the protective effect of a l-carnitine (LC) pretreatment (400 or 880 mg LC/kg bw fish/day, for 21 days) on the histopathological alterations induced by pure CYN or Aphanizomenon ovalisporum lyophilized cells (400 µg CYN/kg bw fish) in liver, kidney, heart, intestines, and gills of tilapia (Oreochromis niloticus) acutely exposed to the toxin by oral route. The main histopathological changes induced by CYN were disorganized parenchyma with presence of glycogen and lipids in the cytoplasm (liver), glomerulonephritis, glomerular atrophy, and dilatation of Bowman's capsule (kidney), myofibrolysis, loss of myofibrils, with edema and hemorrhage (heart), intestinal villi with necrotic enterocytes and partial loss of microvilli (gastrointestinal tract), and hyperemia and hemorrhage (gills). LC pretreatment was able to totally prevent those CYN-induced alterations from 400 mg LC/kg bw fish/day in almost all organs, except in the heart, where 880 mg LC/kg bw fish/day were needed. In addition, the morphometric study indicated that LC managed to recover totally the affectation in the cross sections of the proximal and distal convoluted tubules in CYN-exposed fish. © 2015 Wiley Periodicals, Inc. Environ Toxicol 32: 241-254, 2017.
[Mh] Termos MeSH primário: Toxinas Bacterianas/toxicidade
Carnitina/farmacologia
Rim/efeitos dos fármacos
Fígado/efeitos dos fármacos
Substâncias Protetoras/farmacologia
Uracila/análogos & derivados
Poluentes da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Aphanizomenon/metabolismo
Toxinas Bacterianas/metabolismo
Ciclídeos/metabolismo
Dieta
Brânquias/efeitos dos fármacos
Brânquias/patologia
Coração/efeitos dos fármacos
Rim/patologia
Fígado/patologia
Microscopia Eletrônica
Miocárdio/patologia
Estresse Oxidativo/efeitos dos fármacos
Uracila/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Protective Agents); 0 (Water Pollutants); 2JIZ556BA3 (cylindrospermopsin); 56HH86ZVCT (Uracil); S7UI8SM58A (Carnitine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151231
[St] Status:MEDLINE
[do] DOI:10.1002/tox.22229


  5 / 104 MEDLINE  
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[PMID]:27907126
[Au] Autor:Ballot A; Cerasino L; Hostyeva V; Cirés S
[Ad] Endereço:Norwegian Institute for Water Research, Oslo, Norway.
[Ti] Título:Variability in the sxt Gene Clusters of PSP Toxin Producing Aphanizomenon gracile Strains from Norway, Spain, Germany and North America.
[So] Source:PLoS One;11(12):e0167552, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Paralytic shellfish poisoning (PSP) toxin production has been detected worldwide in the cyanobacterial genera Anabaena, Lyngbya, Scytonema, Cuspidothrix and Aphanizomenon. In Europe Aphanizomenon gracile and Cuspidothrix issatschenkoi are the only known producers of PSP toxins and are found in Southwest and Central European freshwater bodies. In this study the PSP toxin producing Aphanizomenon sp. strain NIVA-CYA 851 was isolated from the Norwegian Lake Hillestadvannet. In a polyphasic approach NIVA-CYA 851 was morphologically and phylogenetically classified, and investigated for toxin production. The strain NIVA-CYA 851 was identified as A. gracile using 16S rRNA gene phylogeny and was confirmed to produce neosaxitoxin, saxitoxin and gonyautoxin 5 by LC-MS. The whole sxt gene clusters (circa 27.3 kb) of four A. gracile strains: NIVA-CYA 851 (Norway); NIVA-CYA 655 & NIVA-CYA 676 (Germany); and UAM 529 (Spain), all from latitudes between 40° and 59° North were sequenced and compared with the sxt gene cluster of reference strain A. gracile NH-5 from the USA. All five sxt gene clusters are highly conserved with similarities exceeding 99.4%, but they differ slightly in the number and presence of single nucleotide polymorphisms (SNPs) and insertions/deletions (In/Dels). Altogether 178 variable sites (44 SNPs and 4 In/Dels, comprising 134 nucleotides) were found in the sxt gene clusters of the Norwegian, German and Spanish strains compared to the reference strain. Thirty-nine SNPs were located in 16 of the 27 coding regions. The sxt gene clusters of NIVA-CYA 851, NIVA-CYA 655, NIVA-CYA 676 and UAM 529, were characterized by 15, 16, 19 and 23 SNPs respectively. Only the Norwegian strain NIVA-CYA 851 possessed an insertion of 126 base pairs (bp) in the noncoding area between the sxtA and sxtE genes and a deletion of 6 nucleotides in the sxtN gene. The sxtI gene showed the highest variability and is recommended as the best genetic marker for further phylogenetic studies of the sxt gene cluster of A. gracile. This study confirms for the first time the role of A. gracile as a PSP toxin producer in Norwegian waters, representing the northernmost occurrence of PSP toxin producing A. gracile in Europe known so far.
[Mh] Termos MeSH primário: Aphanizomenon/genética
Mutação INDEL
Polimorfismo de Nucleotídeo Único
RNA Ribossômico 16S/genética
Saxitoxina/análogos & derivados
Saxitoxina/genética
[Mh] Termos MeSH secundário: Aphanizomenon/classificação
Aphanizomenon/patogenicidade
Organismos Aquáticos
Sequência de Bases
Sequência Conservada
Genes Bacterianos
Alemanha
Lagos/microbiologia
Família Multigênica
Noruega
Fases de Leitura Aberta
Filogenia
Saxitoxina/biossíntese
Espanha
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 35523-89-8 (Saxitoxin); 6YRL8BWD9H (neosaxitoxin); 77462-64-7 (gonyautoxins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0167552


  6 / 104 MEDLINE  
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[PMID]:27738710
[Au] Autor:Zhang SH; Xu PY; Chang JJ
[Ad] Endereço:College of Resources and Environmental Science, South-Central University for Nationalities, Wuhan, 430074, China.
[Ti] Título:Physiological Responses of Aphanizomenon flos-aquae Under the Stress of Sagittaria sagittifolia Extract.
[So] Source:Bull Environ Contam Toxicol;97(6):870-875, 2016 Dec.
[Is] ISSN:1432-0800
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The algal growth and physiological characters of Aphanizomenon flos-aquae were studied under the stress of Sagittaria sagittifolia extract. The results showed that the growth of A. flos-aquae was significantly inhibited by S. sagittifolia extract. The exopolysaccharide (EPS), total soluble protein, intracellular phosphorus (o-PO -P) contents and malondialdehyde (MDA) contents in A. flos-aquae cells increased significantly. These results suggested that A. flos-aquae can adapt to stress by increasing its normal metabolic activity. The algal cellular antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), were triggered to different degrees when exposed to S. sagittifolia extract. The MDA contents and activities of SOD, CAT and POD in algal cells suggested that oxidative damage induced by S. sagittifolia extract via the oxidation of ROS (O · ) might be an important factor responsible for the inhibition of the growth of A. flos-aquae. In addition, SOD may be an important site for the inhibition of S. sagittifolia extract on A. flos-aquae cells. These results indicate that S. sagittifolia may be a good candidate for controlling A. flos-aquae blooms.
[Mh] Termos MeSH primário: Aphanizomenon/efeitos dos fármacos
Aphanizomenon/crescimento & desenvolvimento
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/toxicidade
Sagittaria/toxicidade
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Aphanizomenon/metabolismo
Catalase/metabolismo
Malondialdeído/metabolismo
Peroxidase/metabolismo
Fósforo/metabolismo
Extratos Vegetais/química
Polissacarídeos/metabolismo
Proteínas/metabolismo
Sagittaria/química
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); 0 (Polysaccharides); 0 (Proteins); 27YLU75U4W (Phosphorus); 4Y8F71G49Q (Malondialdehyde); EC 1.11.1.6 (Catalase); EC 1.11.1.7 (Peroxidase); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170127
[Lr] Data última revisão:
170127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161015
[St] Status:MEDLINE


  7 / 104 MEDLINE  
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[PMID]:27696654
[Au] Autor:Klawonn I; Nahar N; Walve J; Andersson B; Olofsson M; Svedén JB; Littmann S; Whitehouse MJ; Kuypers MM; Ploug H
[Ad] Endereço:Department of Ecology, Environment and Plant Sciences Stockholm University, Stockholm, Sweden.
[Ti] Título:Cell-specific nitrogen- and carbon-fixation of cyanobacteria in a temperate marine system (Baltic Sea).
[So] Source:Environ Microbiol;18(12):4596-4609, 2016 Dec.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We analysed N - and carbon (C) fixation in individual cells of Baltic Sea cyanobacteria by combining stable isotope incubations with secondary ion mass spectrometry (SIMS). Specific growth rates based on N - and C-fixation were higher for cells of Dolichospermum spp. than for Aphanizomenon sp. and Nodularia spumigena. The cyanobacterial biomass, however, was dominated by Aphanizomenon sp., which contributed most to total N -fixation in surface waters of the Northern Baltic Proper. N -fixation by Pseudanabaena sp. and colonial picocyanobacteria was not detectable. N -fixation by Aphanizomenon sp., Dolichospermum spp. and N. spumigena populations summed up to total N -fixation, thus these genera appeared as sole diazotrophs within the Baltic Sea's euphotic zone, while their mean contribution to total C-fixation was 21%. Intriguingly, cell-specific N -fixation was eightfold higher at a coastal station compared to an offshore station, revealing coastal zones as habitats with substantial N -fixation. At the coastal station, the cell-specific C- to N -fixation ratio was below the cellular C:N ratio, i.e. N was assimilated in excess to C-fixation, whereas the C- to N -fixation ratio exceeded the C:N ratio in offshore sampled diazotrophs. Our findings highlight SIMS as a powerful tool not only for qualitative but also for quantitative N -fixation assays in aquatic environments.
[Mh] Termos MeSH primário: Ciclo do Carbono
Cianobactérias/metabolismo
Fixação de Nitrogênio
[Mh] Termos MeSH secundário: Aphanizomenon/metabolismo
Países Bálticos
Carbono/metabolismo
Cianobactérias/classificação
Ecossistema
Nitrogênio/metabolismo
Nodularia/metabolismo
Oceanos e Mares
Água do Mar/microbiologia
Espectrometria de Massa de Íon Secundário
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
7440-44-0 (Carbon); N762921K75 (Nitrogen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13557


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[PMID]:27537083
[Au] Autor:Righi V; Parenti F; Schenetti L; Mucci A
[Ad] Endereço:Dipartimento di Scienze per la Qualità della Vita, Università di Bologna , via Corso d'Augusto 237, 47921 Rimini, Italy.
[Ti] Título:Mycosporine-like Amino Acids and Other Phytochemicals Directly Detected by High-Resolution NMR on Klamath (Aphanizomenon flos-aquae) Blue-Green Algae.
[So] Source:J Agric Food Chem;64(35):6708-15, 2016 Sep 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study describes for the first time the use of high-resolution nuclear magnetic resonance (NMR) on Klamath (Aphanizomenon flos-aquae, AFA) blue-green algae directly on powder suspension. These algae are considered to be a "superfood", due to their complete nutritional profile that has proved to have important therapeutic effects. The main advantage of NMR spectroscopy is that it permits the detection of a number of metabolites all at once. The Klamath alga metabolome was revealed to be quite complex, and the most peculiar phytochemicals that can be detected directly on algae by NMR are mycosporine-like amino acids (porphyra-334, P334; shinorine, Shi) and low molecular weight glycosides (glyceryl ß-d-galactopyranoside, GalpG; glyceryl 6-amino-6-deoxy-α-d-glucopyranoside, ADG), all compounds with a high nutraceutical value. The presence of cis-3,4-DhLys was revealed for the first time. This molecule could be involved in the anticancer properties ascribed to AFA.
[Mh] Termos MeSH primário: Aminoácidos/química
Aphanizomenon/química
Cianobactérias/química
[Mh] Termos MeSH secundário: Suplementos Nutricionais
Espectroscopia de Ressonância Magnética/métodos
Compostos Fitoquímicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Phytochemicals)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170419
[Lr] Data última revisão:
170419
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160819
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b02615


  9 / 104 MEDLINE  
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[PMID]:27424100
[Au] Autor:Zhang D; Liu S; Zhang J; Zhang JK; Hu C; Liu Y
[Ad] Endereço:Department of Lifescience and Biotechnology, College of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, PR China. Electronic address: deluzh@163.com.
[Ti] Título:In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill.
[So] Source:Aquat Toxicol;177:484-93, 2016 Aug.
[Is] ISSN:1879-1514
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Aphantoxins, neurotoxins or paralytic shellfish poisons (PSPs) generated by Aphanizomenon flos-aquae, are a threat to environmental safety and human health in eutrophic waters worldwide. The molecular mechanisms of neurotoxin function have been studied; however, the effects of these neurotoxins on oxidative stress, ion transport, gas exchange, and branchial ultrastructure in fish gills are not fully understood. Aphantoxins extracted from A. flos-aquae DC-1 were detected by high-performance liquid chromatography. The major ingredients were gonyautoxins 1 and 5 and neosaxitoxin, which comprised 34.04%, 21.28%, and 12.77% of the total, respectively. Zebrafish (Danio rerio) were administered A. flos-aquae DC-1 aphantoxins at 5.3 or 7.61µg saxitoxin equivalents (eq)/kg (low and high doses, respectively) by intraperitoneal injection. The activities of Na(+)-K(+)-ATPase (NKA), carbonic anhydrase (CA), and lactate dehydrogenase (LDH), ultrastructural alterations in chloride and epithelial cells, and reactive oxygen species (ROS) and total antioxidative capacity (T-AOC) were investigated in the gills during the first 24h after exposure. Aphantoxins significantly increased the level of ROS and decreased the T-AOC in zebrafish gills from 3 to 12h post-exposure, suggesting an induction of oxidative stress and inhibition of antioxidant capacity. Reduced activities of NKA and CA demonstrated abnormal ion transport and gas exchange in the gills of aphantoxin-treated fish. Toxin administration also resulted in increased LDH activity and ultrastructural alterations in chloride and epithelial cells, suggesting a disruption of function and structure in zebrafish gills. The observed abnormalities in zebrafish gills occurred in a time- and dose-dependent manner. These findings demonstrate that aphantoxins or PSPs may inhibit ion transport and gas exchange, increase LDH activity, and result in ultrastructural damage to the gills through elevations in oxidative stress and reduced antioxidant capacity. These effects of aphantoxins in the gills of zebrafish suggest an induction of respiratory toxicity. The parameters investigated in this study may be also considered as biomarkers for studying aphantoxin/PSP exposure and cyanobacterial blooms in nature.
[Mh] Termos MeSH primário: Aphanizomenon/metabolismo
Toxinas Bacterianas/toxicidade
Brânquias/efeitos dos fármacos
Toxinas Marinhas/toxicidade
Poluentes da Água/toxicidade
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Anidrases Carbônicas/metabolismo
Cromatografia Líquida de Alta Pressão
Brânquias/metabolismo
Brânquias/ultraestrutura
Seres Humanos
L-Lactato Desidrogenase/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Espécies Reativas de Oxigênio/metabolismo
ATPase Trocadora de Sódio-Potássio/metabolismo
Proteínas de Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Bacterial Toxins); 0 (Marine Toxins); 0 (Reactive Oxygen Species); 0 (Water Pollutants); 0 (Zebrafish Proteins); 77238-17-6 (aphantoxin); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase); EC 4.2.1.1 (Carbonic Anhydrases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160718
[St] Status:MEDLINE


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[PMID]:27130970
[Au] Autor:Zhang de L; Liu SY; Zhang J; Zhang JK; Hu CX; Liu YD
[Ad] Endereço:Department of Lifescience and Biotechnology, College of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, PR China. Electronic address: deluzh@163.com.
[Ti] Título:Respiratory toxicity of cyanobacterial aphantoxins from Aphanizomenon flos-aquae DC-1 in the zebrafish gill.
[So] Source:Aquat Toxicol;176:106-15, 2016 Jul.
[Is] ISSN:1879-1514
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Aphantoxins from Aphanizomenon flos-aquae are frequently identified in eutrophic waterbodies worldwide. These toxins severely endanger environmental safety and human health due to the production of paralytic shellfish poisons (PSPs). Although the molecular mechanisms of aphantoxin neurotoxicity have been studied, many questions remain to be resolved such as in vivo alterations in branchial histology and neurotransmitter inactivation induced by these neurotoxins. Aphantoxins extracted from a naturally isolated strain of A. flos-aquae DC-1 were determined by high performance liquid chromatography. The basic components of the isolated aphantoxins identified were gonyautoxin 1 (GTX1), gonyautoxin 5 (GTX5), and neosaxitoxin (neoSTX), which comprised 34.04, 21.28, and 12.77% of the total, respectively. Zebrafish (Danio rerio) was administrated 5.3 or 7.61mg STX equivalents (eq)/kg (low and high doses, respectively) of the A. flos-aquae DC-1 aphantoxins by intraperitoneal injection. Histological alterations and changes in neurotransmitter inactivation in the gills of zebrafish were investigated for 24h following exposure. Aphantoxin exposure significantly increased the activities of gill alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and resulted in histological alterations in the gills during the first 12h of exposure, indicating the induction of functional and structural damage. Gill acetylcholinesterase (AChE) and monoamine oxidase (MAO) activities were inhibited significantly, suggesting an alteration of neurotransmitter inactivation in zebrafish gills. The observed alterations in gill structure and function followed a time- and dose-dependent pattern. The results demonstrate that aphantoxins or PSPs lead to structural damage and altered function in the gills of zebrafish, including changes in histological structure and increases in the activities of AST and ALT. The inhibition of the activities of AChE and MAO suggest that aphantoxins or PSPs could induce respiratory toxicity in the zebrafish gill. Furthermore, these parameters may be used as bioindicators for investigating aphantoxin exposure and cyanobacterial blooms in nature.
[Mh] Termos MeSH primário: Aphanizomenon
Toxinas Bacterianas/toxicidade
Brânquias/efeitos dos fármacos
Toxinas Marinhas/toxicidade
Saxitoxina/análogos & derivados
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Acetilcolinesterase/metabolismo
Alanina Transaminase/metabolismo
Animais
Aspartato Aminotransferases/metabolismo
Cromatografia Líquida de Alta Pressão
Brânquias/enzimologia
Brânquias/patologia
Monoaminoxidase/metabolismo
Fenômenos Fisiológicos Respiratórios/efeitos dos fármacos
Saxitoxina/toxicidade
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Marine Toxins); 0 (Water Pollutants, Chemical); 35523-89-8 (Saxitoxin); 6YRL8BWD9H (neosaxitoxin); 77238-17-6 (aphantoxin); 77462-64-7 (gonyautoxins); EC 1.4.3.4 (Monoamine Oxidase); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160501
[St] Status:MEDLINE



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