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Pesquisa : B03.280.210 [Categoria DeCS]
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  1 / 102 MEDLINE  
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[PMID]:28771148
[Au] Autor:Casali SP; Dos Santos ACA; de Falco PB; Calijuri MDC
[Ad] Endereço:Laboratório de Biotoxicologia de Águas Continentais e Efluentes (BIOTACE), Escola de Engenharia do São Carlos, Universidade de São Paulo, Av. Trabalhador São-Carlense, 400. CEP 13566-590 São Carlos, SP, Brazil.
[Ti] Título:Influence of environmental variables on saxitoxin yields by Cylindrospermopsis raciborskii in a mesotrophic subtropical reservoir.
[So] Source:J Water Health;15(4):509-518, 2017 Aug.
[Is] ISSN:1477-8920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Saxitoxins are a class of toxins produced by at least two groups of evolutionarily distant organisms (cyanobacteria and dinoflagellates). While the toxicity of these toxins is relatively well characterized, to date little is known about their drivers and ecological functions, especially in lower latitude tropical and subtropical freshwater ecosystems. In the present study, we aimed to obtain a better understanding of the main drivers of saxitoxin concentrations in aquatic environments. We investigated the relationships among saxitoxin concentrations in a mesotrophic subtropical reservoir dominated by the cyanobacteria Cylindrospermopsis raciborskii with physical, chemical and biological water variables. The highest saxitoxin concentrations were 0.20 µg·L , which occurred in the samples with the highest densities of C. raciborskii (maximum of 4.3 × 10 org·mL ) and the highest concentration of dissolved nutrients (nitrate from 0.2 to 0.8 µg·L , ortophosphate from 0.3 to 8.5 µg·L ). These correlations were confirmed by statistical analyses. However, the highest saxitoxin relative concentrations (per trichome) were associated with lower C. raciborskii densities, suggesting that saxitoxin production or the selection of saxitoxin-producing strains was associated with the adaptation of this species to conditions of stress. Our results indicate that C. raciborskii toxin yields vary depending on the enrichment conditions having potential implications for reservoir management.
[Mh] Termos MeSH primário: Toxinas Bacterianas/análise
Cylindrospermopsis/química
Lagos/microbiologia
Saxitoxina/análise
[Mh] Termos MeSH secundário: Brasil
Meio Ambiente
Monitoramento Ambiental
Fitoplâncton/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 35523-89-8 (Saxitoxin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.2166/wh.2017.266


  2 / 102 MEDLINE  
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Marinho, Marcelo Manzi
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[PMID]:28423343
[Au] Autor:Mucci M; Noyma NP; de Magalhães L; Miranda M; van Oosterhout F; Guedes IA; Huszar VLM; Marinho MM; Lürling M
[Ad] Endereço:Aquatic Ecology & Water Quality Management Group, Department of Environmental Sciences, Wageningen University, P.O. Box 47, 6700 AA, Wageningen, The Netherlands. Electronic address: maira.mucci@wur.nl.
[Ti] Título:Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis.
[So] Source:Water Res;118:121-130, 2017 Jul 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Combining coagulant and ballast to remove cyanobacteria from the water column is a promising restoration technique to mitigate cyanobacterial nuisance in surface waters. The organic, biodegradable polymer chitosan has been promoted as a coagulant and is viewed as non-toxic. In this study, we show that chitosan may rapidly compromise membrane integrity and kill certain cyanobacteria leading to release of cell contents in the water. A strain of Cylindrospermopsis raciborskii and one strain of Planktothrix agardhii were most sensitive. A 1.3 h exposure to a low dose of 0.5 mg l chitosan already almost completely killed these cultures resulting in release of cell contents. After 24 h, reductions in PSII efficiencies of all cyanobacteria tested were observed. EC50 values varied from around 0.5 mg l chitosan for the two sensitive strains, via about 5 mg l chitosan for an Aphanizomenon flos-aquae strain, a toxic P. agardhii strain and two Anabaena cylindrica cultures, to more than 8 mg l chitosan for a Microcystis aeruginosa strain and another A. flos-aquae strain. Differences in sensitivity to chitosan might be related to polymeric substances that surround cyanobacteria. Rapid lysis of toxic strains is likely and when chitosan flocking and sinking of cyanobacteria is considered in lake restoration, flocculation efficacy studies should be complemented with investigation on the effects of chitosan on the cyanobacteria assemblage being targeted.
[Mh] Termos MeSH primário: Quitosana
Cianobactérias
Eutrofização
[Mh] Termos MeSH secundário: Cylindrospermopsis
Lagos
Microcystis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9012-76-4 (Chitosan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE


  3 / 102 MEDLINE  
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[PMID]:28334256
[Au] Autor:Kokocinski M; Gagala I; Jasser I; Karosiene J; Kasperoviciene J; Kobos J; Koreiviene J; Soininen J; Szczurowska A; Woszczyk M; Mankiewicz-Boczek J
[Ad] Endereço:Department of Hydrobiology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.
[Ti] Título:Distribution of invasive Cylindrospermopsis raciborskii in the East-Central Europe is driven by climatic and local environmental variables.
[So] Source:FEMS Microbiol Ecol;93(4), 2017 Apr 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mechanisms behind expansion of an invasive cyanobacterium Cylindrospermopsis raciborskii have not been fully resolved, and different hypotheses, such as global warming, are suggested. In the East-Central Europe, it is widely occurring in western part of Poland but only in single locations in the East due to some limiting factors. Therefore, broad-scale phytoplankton survey including 117 randomly selected lakes in Poland and Lithuania was conducted. The results showed that C. raciborskii occurred widely in western part of Poland but was absent from other regions and Lithuania except one lake. The regions in which C. raciborskii was present had higher annual mean air temperature, higher maximum air temperature of the warmest month and higher minimum temperature of the coldest month, demonstrating that average air temperature, and indirectly, the duration of growing season might be more important factor driving C. raciborskii distribution than measured in situ water temperature. In turn, the presence of C. raciborskii in single localities may be more related to physiological adaptations of separated ecotype. Collectively, these results provide novel evidence on the influence of temperature on C. raciborskii distribution in East-European regions but also indicate high ecological plasticity of this species.
[Mh] Termos MeSH primário: Cylindrospermopsis/fisiologia
Espécies Introduzidas
Microbiologia da Água
[Mh] Termos MeSH secundário: Cianobactérias/fisiologia
Cylindrospermopsis/isolamento & purificação
Monitoramento Ambiental
Europa (Continente)
Aquecimento Global
Lagos/microbiologia
Lituânia
Fitoplâncton
Polônia
Estações do Ano
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix035


  4 / 102 MEDLINE  
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[PMID]:28202362
[Au] Autor:Lopes KC; Ferrão-Filho AD; Dos Santos EG; Cunha RA; Santos CP
[Ad] Endereço:Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil, 4365 - Manguinhos, Rio de Janeiro, RJ 21040-360, Brazil; Pós-Graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
[Ti] Título:Effects of crude extracts of a saxitoxin-producer strain of the cyanobacterium Cylindrospermopsis raciborskii on the swimming behavior of wild and laboratory reared guppy Poecilia vivipara.
[So] Source:Toxicon;129:44-51, 2017 Apr.
[Is] ISSN:1879-3150
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The cyanobacterium Cylindrospermopsis raciborskii is an invasive species in water supply reservoirs worldwide, which can produces cylindrospermopsins and saxitoxins. In the wild, guppy (Poecilia vivipara) can be exposed to cyanotoxins, but those born and reared in laboratory are free of this contact. The aim of this paper was to comparatively measure the locomotor activity of 'wild' and 'lab' P. vivipara before and after exposure to crude extracts of two different cultures of C. raciborskii (CYRF-01), a saxitoxin-procucer strain. The movement of each fish was recorded using an image monitoring system (Videomex V ) before and after 48 h exposure to cyanobacterial extracts. Each experiment was performed during 4 h, with 1 h acclimation and 3 h recording period of the parameters Distance performed (DP), Swimming time (SwT), Stereotypic time (StT), Resting time (RT) and Average speed (AS). The quantification of saxitoxin in the solutions was performed by the enzyme-linked immunosorbent assay (ELISA). The weight or the total length did not influence the locomotor activity of fish in any of the experiments. The saxitoxin value was similar for both cultures (Culture 1: 7.3 µg L and Culture 2: 8.6 µg L ). However, in experiments with Culture 1 an increased activity in most parameters was observed, while in Culture 2, a decreased activity was observed only in 'lab' fish. Wild fish was less affected, showing higher resistance to both cyanobacterial crude extracts. This study showed that different cultures of the same strain of C. raciborskii and with similar contents of saxitoxin are able to change the locomotor activity of P. vivipara, contributing to the validation of the use of behavioral parameters to the evaluation of sublethal effects of toxic cyanobacteria on fish.
[Mh] Termos MeSH primário: Comportamento Animal/efeitos dos fármacos
Cylindrospermopsis/química
Poecilia
Saxitoxina/toxicidade
Natação
[Mh] Termos MeSH secundário: Animais
Toxinas Bacterianas/toxicidade
Ensaio de Imunoadsorção Enzimática
Uracila/análogos & derivados
Uracila/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 2JIZ556BA3 (cylindrospermopsin); 35523-89-8 (Saxitoxin); 56HH86ZVCT (Uracil)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE


  5 / 102 MEDLINE  
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[PMID]:27352231
[Au] Autor:Svircev Z; Obradovic V; Codd GA; Marjanovic P; Spoof L; Drobac D; Tokodi N; Petkovic A; Nenin T; Simeunovic J; Vazic T; Meriluoto J
[Ad] Endereço:Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovica 2, Novi Sad, 21000, Serbia.
[Ti] Título:Massive fish mortality and Cylindrospermopsis raciborskii bloom in Aleksandrovac Lake.
[So] Source:Ecotoxicology;25(7):1353-63, 2016 Sep.
[Is] ISSN:1573-3017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This paper presents a case study of a massive fish mortality during a Cylindrospermopsis raciborskii bloom in Aleksandrovac Lake, Serbia in mid-December 2012. According to a preliminary investigation of the samples taken on November 6 before the fish mortalities and to extended analyses of samples taken on November 15, no values of significant physicochemical parameters emerged to explain the cause(s) of the fish mortality. No industrial pollutants were apparent at this location, and results excluded the likelihood of bacterial infections. Even after freezing, the dissolved oxygen concentration in the water was sufficient for fish survival. High concentrations of chlorophyll a and phaeophytin occurred in the lake, and phytoplankton bloom samples were lethal in Artemia salina bioassays. A bloom of the cyanobacterium C. raciborskii was recorded during November. Although the A. salina bioassays indicated the presence of toxic compounds in the cyanobacterial cells, the cyanotoxins, microcystins, cylindrospermopsin and saxitoxin were not detected.
[Mh] Termos MeSH primário: Cylindrospermopsis/crescimento & desenvolvimento
Monitoramento Ambiental
Peixes/fisiologia
Proliferação Nociva de Algas
Lagos/microbiologia
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Toxinas Bacterianas/toxicidade
Microcistinas
Uracila/análogos & derivados
Uracila/toxicidade
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Microcystins); 0 (Water Pollutants, Chemical); 2JIZ556BA3 (cylindrospermopsin); 56HH86ZVCT (Uracil)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160629
[St] Status:MEDLINE
[do] DOI:10.1007/s10646-016-1687-x


  6 / 102 MEDLINE  
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[PMID]:27191577
[Au] Autor:Tonietto AE; Oliveira NL; Lombardi AT; Polpo A
[Ad] Endereço:Department of Botany, Federal University of São Carlos, Via Washington Luis km 235, PO Box 676, São Carlos, São Paulo CEP 13565-905, Brazil E-mail: aletonietto@gmail.com.
[Ti] Título:Copper and cadmium complexation by Cylindrospermopsis raciborskii exudates.
[So] Source:Water Sci Technol;73(10):2544-51, 2016.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cylindrospermopsis raciborskii is a potentially toxic cyanobacterium that excretes organic materials which act as ligands for metals. Metal ligands may be characterized for their strength of association, e.g., stability constants, which can be either thermodynamic (K) or conditional (K'). In this research we examined K and K' for Cu and Cd complexes with three molecular weight fractions (>30 kDa; 30-10 kDa; 10-3 kDa) of the cyanobacteria EOM. Complexation capacities of the excreted organic materials (EOM) for metals were determined at several ionic strengths (1.0 × 10(-2), 5.0 × 10(-2), 1.0 × 10(-1), and 5.0 × 10(-1) mol L(-1)) at pH 6.6 ± 0.1, with ligands for which no data for their acidity constants are available; these constants are thus conditional for this specific pH. Bayesian statistics showed that with a probability of 95-100% the EOM have two different ligands for Cu but only one for Cd, that ligands for Cu were stronger than for Cd (94-100% probability), and that the smallest EOM fraction had the highest strength of association for Cu (logKCuL 13.5). The lowest affinity was obtained for Cd (logKCdL 8.6) complexed to any molecular weight fraction. The present findings have important ecological implications, since the metal-ligand association is dynamic, and together with a diversity of ligands it can act as an environmental metal buffer. As a result, higher metal loads may be necessary for the detection of toxicity.
[Mh] Termos MeSH primário: Cádmio/química
Cobre/química
Cylindrospermopsis/metabolismo
Compostos Orgânicos/química
[Mh] Termos MeSH secundário: Teorema de Bayes
Ligantes
Peso Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ligands); 0 (Organic Chemicals); 00BH33GNGH (Cadmium); 789U1901C5 (Copper)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160519
[Lr] Data última revisão:
160519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160519
[St] Status:MEDLINE
[do] DOI:10.2166/wst.2016.111


  7 / 102 MEDLINE  
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PubMed Central Texto completo
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[PMID]:26888523
[Au] Autor:Rangel LM; Ger KA; Silva LH; Soares MC; Faassen EJ; Lürling M
[Ad] Endereço:Departamento de Botânica - Museu Nacional, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 20940-040, Brazil. luciana.rangel@gmail.com.
[Ti] Título:Toxicity Overrides Morphology on Cylindrospermopsis raciborskii Grazing Resistance to the Calanoid Copepod Eudiaptomus gracilis.
[So] Source:Microb Ecol;71(4):835-44, 2016 May.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Toxicity and morphology may function as defense mechanisms of bloom-forming cyanobacteria against zooplankton grazing. Yet, the relative importance of each of these factors and their plasticity remains poorly known. We tested the effects of chemical and morphological traits of the bloom-forming cyanobacterium Cylindrospermopsis raciborskii on the feeding response of the selective feeder Eudiaptomus gracilis (Calanoida, Copepoda), using a saxitoxin-producing strain (STX+) and a non-saxitoxin (STX-)-producing strain as food. From these two chemotypes, we established cultures of three different morphotypes that differed in filament length (short, medium, and long) by incubating the strains at 17, 25, and 32 °C. We hypothesized that the inhibitory effects of saxitoxins determine the avoidance of C. raciborskii, and that morphology would only become relevant in the absence of saxitoxins. Temperature affected two traits: higher temperature resulted in significantly shorter filaments in both strains and led to much higher toxin contents in the STX+ strain (1.7 µg eq STX L(-1) at 17 °C, 7.9 µg eq STX L(-1) at 25 °C, and 25.1 µg eq STX L(-1) at 32 °C). Copepods strongly reduced the ingestion of the STX+ strain in comparison with STX- cultures, regardless of filament length. Conversely, consumption of shorter filaments was significantly higher in the STX- strain. The great plasticity of morphological and chemical traits of C. raciborskii and their resultant contrasting effects on the feeding behavior of zooplankton might explain the success of this cyanobacterium in a variety of aquatic environments.
[Mh] Termos MeSH primário: Copépodes/fisiologia
Cylindrospermopsis/metabolismo
Água Doce/microbiologia
[Mh] Termos MeSH secundário: Animais
Cylindrospermopsis/química
Cylindrospermopsis/citologia
Comportamento Alimentar/fisiologia
Lagos/microbiologia
Fitoplâncton/fisiologia
Saxitoxina/biossíntese
Saxitoxina/toxicidade
Zooplâncton/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
35523-89-8 (Saxitoxin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160219
[St] Status:MEDLINE
[do] DOI:10.1007/s00248-016-0734-8


  8 / 102 MEDLINE  
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[PMID]:26546759
[Au] Autor:Capelo-Neto J; Silva Buarque NM
[Ad] Endereço:Federal University of Ceara - DEHA, Bloco 713 - 1, Andar CEP 60451-970, Brazil. Electronic address: zecapelo@hotmail.com.
[Ti] Título:Simulation of saxitoxins adsorption in full-scale GAC filter using HSDM.
[So] Source:Water Res;88:558-565, 2016 Jan 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many different species of cyanobacteria capable of producing saxitoxins have been identified as a threat to the safety of drinking water supplies worldwide. Removal of these contaminants can be accomplished by adsorption on granular activated carbon (GAC) but little is yet known about the kinetics of this process. This research investigated adsorption kinetics and diffusion behaviour of decarbomoyl saxitoxin (dc-STX) and carbamate saxitoxin (STX) on a GAC sample and simulated a full-scale GAC filter using batch experimental data and the homogeneous surface diffusion model (HSDM). HSDM was able to successfully describe batch adsorption of STX and dc-STX onto GAC sample and the surface diffusion coefficient was identified as the main adjustment parameter for this model. Different scenarios of STX and dc-STX removal in a GAC filter were simulated, offering engineers and scientists an option for the design of GAC full-scale filters, bench or pilot-scale experiments.
[Mh] Termos MeSH primário: Saxitoxina/química
Poluentes Químicos da Água/química
Purificação da Água
[Mh] Termos MeSH secundário: Adsorção
Cylindrospermopsis/química
Difusão
Filtração
Cinética
Modelos Teóricos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 35523-89-8 (Saxitoxin)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151108
[St] Status:MEDLINE


  9 / 102 MEDLINE  
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[PMID]:26347118
[Au] Autor:Ongley SE; Pengelly JJ; Neilan BA
[Ad] Endereço:School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia.
[Ti] Título:Elevated Na(+) and pH influence the production and transport of saxitoxin in the cyanobacteria Anabaena circinalis AWQC131C and Cylindrospermopsis raciborskii T3.
[So] Source:Environ Microbiol;18(2):427-38, 2016 Feb.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Saxitoxins (STX), neurotoxic alkaloids, fall under the umbrella of paralytic shellfish toxins produced by marine dinoflagellates and freshwater cyanobacteria. The genes responsible for the production of STX have been proposed, but factors that influence their expression and induce toxin efflux remain unclear. Here we characterize the putative STX NorM-like MATE transporters SxtF and SxtM. Complementation of the antibiotic-sensitive strain Escherichia coli KAM32 with these transporters decreased fluoroquinolone sensitivity, indicating that while becoming evolutionary specialized for STX transport these transporters retain relaxed specificity typical of this class. The transcriptional response of STX biosynthesis (sxtA) along with that of the STX transporters (sxtM and sxtF from Cylindrospermopsis raciborskii T3, and sxtM from Anabaena circinalis AWQC131C) were assessed in response to ionic stress. These data, coupled with a measure of toxin intracellular to extracellular ratios, provide an insight into the physiology of STX export. Cylindrospermopsis raciborskii and Anabaena circinalis exhibited opposing responses under conditions of ionic stress. High Na(+) (10 mM) induced moderate alterations of transcription and STX localization, whereas high pH (pH 9) stimulated the greatest physiological response. Saxitoxin production and cellular localization are responsive to ionic strength, indicating a role of this molecule in the maintenance of cellular homeostasis.
[Mh] Termos MeSH primário: Anabaena/metabolismo
Cylindrospermopsis/metabolismo
Escherichia coli/genética
Fluoroquinolonas/farmacologia
Saxitoxina/metabolismo
Sódio/metabolismo
[Mh] Termos MeSH secundário: Transporte Biológico Ativo/genética
Transporte Biológico Ativo/fisiologia
Dinoflagelados/metabolismo
Farmacorresistência Bacteriana/genética
Escherichia coli/efeitos dos fármacos
Escherichia coli/metabolismo
Fluoroquinolonas/metabolismo
Água Doce
Concentração de Íons de Hidrogênio
Íons/metabolismo
Proteínas de Membrana Transportadoras/genética
Proteínas de Membrana Transportadoras/metabolismo
Testes de Sensibilidade Microbiana
Estresse Fisiológico/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluoroquinolones); 0 (Ions); 0 (Membrane Transport Proteins); 35523-89-8 (Saxitoxin); 9NEZ333N27 (Sodium)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150909
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13048


  10 / 102 MEDLINE  
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[PMID]:26164741
[Au] Autor:Piccini C; Fabre A; Lacerot G; Bonilla S
[Ad] Endereço:Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Uruguay; Grupo Ecología y Fisiología de Fitoplancton, CSIC, Universidad de la República, Uruguay. Electronic address: cpiccini@iibce.edu.uy.
[Ti] Título:Combining immunolabeling and catalyzed reporter deposition to detect intracellular saxitoxin in a cyanobacterium.
[So] Source:J Microbiol Methods;117:18-21, 2015 Oct.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We combined the use of polyclonal antibodies against saxitoxin with catalyzed reporter deposition to detect production of saxitoxin by the cyanobacterium Cylindrospermopsis raciborskii. The procedure is simple, allows detection of intracellular saxitoxin in cyanobacteria filaments by confocal laser microscopy and is a promising tool to study toxin production and metabolism.
[Mh] Termos MeSH primário: Cylindrospermopsis/metabolismo
Imuno-Histoquímica/métodos
Saxitoxina/análise
Saxitoxina/metabolismo
[Mh] Termos MeSH secundário: Técnicas Bacteriológicas
Cylindrospermopsis/química
Microscopia Confocal
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
35523-89-8 (Saxitoxin)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150919
[Lr] Data última revisão:
150919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150713
[St] Status:MEDLINE



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