Base de dados : MEDLINE
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[PMID]:28207180
[Au] Autor:Stewart GC
[Ad] Endereço:Department of Veterinary Pathobiology and Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA.
[Ti] Título:Assembly of the outermost spore layer: pieces of the puzzle are coming together.
[So] Source:Mol Microbiol;104(4):535-538, 2017 05.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Certain endospore-forming soil dwelling bacteria are important human, animal or insect pathogens. These organisms produce spores containing an outer layer, the exosporium. The exosporium is the site of interactions between the spore and the soil environment and between the spore and the infected host during the initial stages of infection. The composition and assembly process of the exosporium are poorly understood. This is partly due to the extreme stability of the exosporium that has proven to be refractive to existing methods to deconstruct the intact structure into its component parts. Although more than 20 proteins have been identified as exosporium-associated, their abundance, relationship to other proteins and the processes by which they are assembled to create the exosporium are largely unknown. In this issue of Molecular Microbiology, Terry, Jiang, and colleagues in Per Bullough's laboratory show that the ExsY protein is a major structural protein of the exosporium basal layer of B. cereus family spores and that it can self-assemble into complex structures that possess many of the structural features characteristic of the exosporium basal layer. The authors refined a model for exosporium assembly. Their findings may have implications for exosporium formation in other spore forming bacteria, including Clostridium species.
[Mh] Termos MeSH primário: Esporos Bacterianos/metabolismo
[Mh] Termos MeSH secundário: Bacillus cereus/metabolismo
Proteínas de Bactérias/metabolismo
Parede Celular/metabolismo
Bactérias Formadoras de Endosporo
Microbiologia do Solo
[Pt] Tipo de publicação:INTRODUCTORY JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; COMMENT
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13651


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[PMID]:26511622
[Au] Autor:de Almeida Couto CR; Alvarez VM; Marques JM; de Azevedo Jurelevicius D; Seldin L
[Ad] Endereço:Laboratório de Genética Microbiana, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Ilha do Fundão, Rio de Janeiro, RJ, CEP 21941-590, Brazil. camilarattes@gmail.com.
[Ti] Título:Exploiting the aerobic endospore-forming bacterial diversity in saline and hypersaline environments for biosurfactant production.
[So] Source:BMC Microbiol;15:240, 2015 Oct 28.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Biosurfactants are surface-active biomolecules with great applicability in the food, pharmaceutical and oil industries. Endospore-forming bacteria, which survive for long periods in harsh environments, are described as biosurfactant producers. Although the ubiquity of endospore-forming bacteria in saline and hypersaline environments is well known, studies on the diversity of the endospore-forming and biosurfactant-producing bacterial genera/species in these habitats are underrepresented. METHODS: In this study, the structure of endospore-forming bacterial communities in sediment/mud samples from Vermelha Lagoon, Massambaba, Dois Rios and Abraão Beaches (saline environments), as well as the Praia Seca salterns (hypersaline environments) was determined via denaturing gradient gel electrophoresis. Bacterial strains were isolated from these environmental samples and further identified using 16S rRNA gene sequencing. Strains presenting emulsification values higher than 30 % were grouped via BOX-PCR, and the culture supernatants of representative strains were subjected to high temperatures and to the presence of up to 20 % NaCl to test their emulsifying activities in these extreme conditions. Mass spectrometry analysis was used to demonstrate the presence of surfactin. RESULTS: A diverse endospore-forming bacterial community was observed in all environments. The 110 bacterial strains isolated from these environmental samples were molecularly identified as belonging to the genera Bacillus, Thalassobacillus, Halobacillus, Paenibacillus, Fictibacillus and Paenisporosarcina. Fifty-two strains showed emulsification values of at least 30%, and they were grouped into 18 BOX groups. The stability of the emulsification values varied when the culture supernatants of representative strains were subjected to high temperatures and to the presence of up to 20% NaCl. The presence of surfactin was demonstrated in one of the most promising strains. CONCLUSION: The environments studied can harbor endospore-forming bacteria capable of producing biosurfactants with biotechnological applications. Various endospore-forming bacterial genera/species are presented for the first time as biosurfactant producers.
[Mh] Termos MeSH primário: Bactérias Aeróbias/isolamento & purificação
Bactérias Aeróbias/metabolismo
Bactérias Formadoras de Endosporo/isolamento & purificação
Bactérias Formadoras de Endosporo/metabolismo
Microbiologia Ambiental
Tensoativos/metabolismo
[Mh] Termos MeSH secundário: Bactérias Aeróbias/classificação
Bactérias Aeróbias/genética
Brasil
Análise por Conglomerados
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Bactérias Formadoras de Endosporo/classificação
Bactérias Formadoras de Endosporo/genética
Espectrometria de Massas
Dados de Sequência Molecular
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Cloreto de Sódio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 0 (Surface-Active Agents); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151030
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-015-0575-5


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[PMID]:26449556
[Au] Autor:Qian Y; Kando CK; Thorsen L; Larsen N; Jespersen L
[Ad] Endereço:Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark yangqian@food.ku.dk.
[Ti] Título:Production of autoinducer-2 by aerobic endospore-forming bacteria isolated from the West African fermented foods.
[So] Source:FEMS Microbiol Lett;362(22), 2015 Nov.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Autoinducer-2 (AI-2) is a quorum-sensing (QS) molecule which mediates interspecies signaling and affects various bacterial behaviors in food fermentation. Biosynthesis of AI-2 is controlled by S-ribosylhomocysteine lyase encoded by the luxS gene. The objective of this study was to investigate production of AI-2 by aerobic endospore-forming bacteria (AEB) isolated from the West African alkaline fermented seed products Mantchoua and Maari. The study included 13 AEB strains of Bacillus subtilis, B. cereus, B. altitudinis, B. amyloliquefaciens, B. licheniformis, B. aryabhattai, B. safensis, Lysinibacillus macroides and Paenibacillus polymyxa. All the tested strains harbored the luxS gene and all strains except for P. polymyxa B314 were able to produce AI-2 during incubation in laboratory medium. Production of AI-2 by AEB was growth phase dependent, showing maximum activity at the late exponential phase. AI-2 was depleted from the culture medium at the beginning of the stationary growth phase, indicating that the tested AEB possess a functional AI-2 receptor that internalizes AI-2. This study provides the evidences of QS system in Bacillus spp. and L. macroides and new knowledge of AI-2 production by AEB. This knowledge contributes to the development of QS-based strategies for better control of alkaline fermentation.
[Mh] Termos MeSH primário: Bacillaceae/metabolismo
Bactérias Aeróbias/metabolismo
Bactérias Formadoras de Endosporo/metabolismo
Microbiologia de Alimentos
Homosserina/análogos & derivados
Lactonas/metabolismo
Paenibacillus/metabolismo
[Mh] Termos MeSH secundário: África Ocidental
Bacillaceae/genética
Bacillaceae/crescimento & desenvolvimento
Bacillaceae/isolamento & purificação
Bactérias Aeróbias/genética
Bactérias Aeróbias/crescimento & desenvolvimento
Bactérias Aeróbias/isolamento & purificação
Proteínas de Bactérias/genética
Transporte Biológico
Liases de Carbono-Enxofre/genética
Meios de Cultura/química
Bactérias Formadoras de Endosporo/genética
Bactérias Formadoras de Endosporo/crescimento & desenvolvimento
Bactérias Formadoras de Endosporo/isolamento & purificação
Fermentação
Homosserina/metabolismo
Paenibacillus/genética
Paenibacillus/crescimento & desenvolvimento
Paenibacillus/isolamento & purificação
Percepção de Quorum
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Culture Media); 0 (Lactones); 0 (N-octanoylhomoserine lactone); 6KA95X0IVO (Homoserine); EC 4.4.- (Carbon-Sulfur Lyases); EC 4.4.1.21 (LuxS protein, Bacteria)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:151028
[Lr] Data última revisão:
151028
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151010
[St] Status:MEDLINE


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[PMID]:26104459
[Au] Autor:Adams V; Li J; Wisniewski JA; Uzal FA; Moore RJ; McClane BA; Rood JI
[Ti] Título:Virulence Plasmids of Spore-Forming Bacteria.
[So] Source:Microbiol Spectr;2(6), 2014 Dec.
[Is] ISSN:2165-0497
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plasmid-encoded virulence factors are important in the pathogenesis of diseases caused by spore-forming bacteria. Unlike many other bacteria, the most common virulence factors encoded by plasmids in Clostridium and Bacillus species are protein toxins. Clostridium perfringens causes several histotoxic and enterotoxin diseases in both humans and animals and produces a broad range of toxins, including many pore-forming toxins such as C. perfringens enterotoxin, epsilon-toxin, beta-toxin, and NetB. Genetic studies have led to the determination of the role of these toxins in disease pathogenesis. The genes for these toxins are generally carried on large conjugative plasmids that have common core replication, maintenance, and conjugation regions. There is considerable functional information available about the unique tcp conjugation locus carried by these plasmids, but less is known about plasmid maintenance. The latter is intriguing because many C. perfringens isolates stably maintain up to four different, but closely related, toxin plasmids. Toxin genes may also be plasmid-encoded in the neurotoxic clostridia. The tetanus toxin gene is located on a plasmid in Clostridium tetani, but the botulinum toxin genes may be chromosomal, plasmid-determined, or located on bacteriophages in Clostridium botulinum. In Bacillus anthracis it is well established that virulence is plasmid determined, with anthrax toxin genes located on pXO1 and capsule genes on a separate plasmid, pXO2. Orthologs of these plasmids are also found in other members of the Bacillus cereus group such as B. cereus and Bacillus thuringiensis. In B. thuringiensis these plasmids may carry genes encoding one or more insecticidal toxins.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Toxinas Bacterianas/genética
Bactérias Formadoras de Endosporo/genética
Plasmídeos
Fatores de Virulência/genética
[Mh] Termos MeSH secundário: Bactérias Formadoras de Endosporo/patogenicidade
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (Virulence Factors)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150624
[Lr] Data última revisão:
150624
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150625
[St] Status:MEDLINE
[do] DOI:10.1128/microbiolspec.PLAS-0024-2014


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[PMID]:26104376
[Au] Autor:Hutchison EA; Miller DA; Angert ER
[Ti] Título:Sporulation in Bacteria: Beyond the Standard Model.
[So] Source:Microbiol Spectr;2(5), 2014 Oct.
[Is] ISSN:2165-0497
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Endospore formation follows a complex, highly regulated developmental pathway that occurs in a broad range of Firmicutes. Although Bacillus subtilis has served as a powerful model system to study the morphological, biochemical, and genetic determinants of sporulation, fundamental aspects of the program remain mysterious for other genera. For example, it is entirely unknown how most lineages within the Firmicutes regulate entry into sporulation. Additionally, little is known about how the sporulation pathway has evolved novel spore forms and reproductive schemes. Here, we describe endospore and internal offspring development in diverse Firmicutes and outline progress in characterizing these programs. Moreover, comparative genomics studies are identifying highly conserved sporulation genes, and predictions of sporulation potential in new isolates and uncultured bacteria can be made from these data. One surprising outcome of these comparative studies is that core regulatory and some structural aspects of the program appear to be universally conserved. This suggests that a robust and sophisticated developmental framework was already in place in the last common ancestor of all extant Firmicutes that produce internal offspring or endospores. The study of sporulation in model systems beyond B. subtilis will continue to provide key information on the flexibility of the program and provide insights into how changes in this developmental course may confer advantages to cells in diverse environments.
[Mh] Termos MeSH primário: Bactérias Formadoras de Endosporo/crescimento & desenvolvimento
Firmicutes/crescimento & desenvolvimento
Esporos Bacterianos/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Bactérias Formadoras de Endosporo/genética
Bactérias Formadoras de Endosporo/metabolismo
Firmicutes/genética
Firmicutes/metabolismo
Regulação Bacteriana da Expressão Gênica
Modelos Biológicos
Transdução de Sinais
Esporos Bacterianos/genética
Esporos Bacterianos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; REVIEW
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150624
[Lr] Data última revisão:
150624
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150625
[St] Status:MEDLINE
[do] DOI:10.1128/microbiolspec.TBS-0013-2012


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[PMID]:24548930
[Au] Autor:Jeong DW; Kim HR; Jung G; Han S; Kim CT; Lee JH
[Ad] Endereço:Department of Food Science and Biotechnlogy, Kyonggi University, Suwon 443-760, Republic of Korea.
[Ti] Título:Bacterial community migration in the ripening of doenjang, a traditional Korean fermented soybean food.
[So] Source:J Microbiol Biotechnol;24(5):648-60, 2014 May.
[Is] ISSN:1738-8872
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Doenjang, a traditional Korean fermented soybean paste, is made by mixing and ripening meju with high salt brine (approximately 18%). Meju is a naturally fermented soybean block prepared by soaking, steaming, and molding soybean. To understand living bacterial community migration and the roles of bacteria in the manufacturing process of doenjang, the diversity of culturable bacteria in meju and doenjang was examined using media supplemented with NaCl, and some physiological activities of predominant isolates were determined. Bacilli were the major bacteria involved throughout the entire manufacturing process from meju to doenjang; some of these bacteria might be present as spores during the doenjang ripening process. Bacillus siamensis was the most populous species of the genus, and Bacillus licheniformis exhibited sufficient salt tolerance to maintain its growth during doenjang ripening. Enterococcus faecalis and Enterococcus faecium, the major lactic acid bacteria (LAB) identified in this study, did not continue to grow under high NaCl conditions in doenjang. Enterococci and certain species of coagulase-negative staphylococci (CNS) were the predominant acid-producing bacteria in meju fermentation, whereas Tetragenococcus halophilus and CNS were the major acid-producing bacteria in doenjang fermentation. We conclude that bacilli, LAB, and CNS may be the major bacterial groups involved in meju fermentation and that these bacterial communities undergo a shift toward salt-tolerant bacilli, CNS, and T. halophilus during the doenjang fermentation process.
[Mh] Termos MeSH primário: Bactérias/classificação
Fermentação
Microbiologia de Alimentos
Alimentos de Soja/microbiologia
[Mh] Termos MeSH secundário: Ácidos/metabolismo
Bacillus/classificação
Bactérias/efeitos dos fármacos
Bactérias/crescimento & desenvolvimento
Carga Bacteriana
Fenômenos Fisiológicos Bacterianos
Proteínas de Bactérias/metabolismo
Bactérias Formadoras de Endosporo
Enzimas/metabolismo
Microbiota
Cloreto de Sódio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acids); 0 (Bacterial Proteins); 0 (Enzymes); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140220
[St] Status:MEDLINE


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[PMID]:23123912
[Au] Autor:Traag BA; Pugliese A; Eisen JA; Losick R
[Ad] Endereço:Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.
[Ti] Título:Gene conservation among endospore-forming bacteria reveals additional sporulation genes in Bacillus subtilis.
[So] Source:J Bacteriol;195(2):253-60, 2013 Jan.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The capacity to form endospores is unique to certain members of the low-G+C group of Gram-positive bacteria (Firmicutes) and requires signature sporulation genes that are highly conserved across members of distantly related genera, such as Clostridium and Bacillus. Using gene conservation among endospore-forming bacteria, we identified eight previously uncharacterized genes that are enriched among endospore-forming species. The expression of five of these genes was dependent on sporulation-specific transcription factors. Mutants of none of the genes exhibited a conspicuous defect in sporulation, but mutants of two, ylxY and ylyA, were outcompeted by a wild-type strain under sporulation-inducing conditions, but not during growth. In contrast, a ylmC mutant displayed a slight competitive advantage over the wild type specific to sporulation-inducing conditions. The phenotype of a ylyA mutant was ascribed to a defect in spore germination efficiency. This work demonstrates the power of combining phylogenetic profiling with reverse genetics and gene-regulatory studies to identify unrecognized genes that contribute to a conserved developmental process.
[Mh] Termos MeSH primário: Bacillus subtilis/crescimento & desenvolvimento
Bacillus subtilis/genética
Bactérias Formadoras de Endosporo/crescimento & desenvolvimento
Bactérias Formadoras de Endosporo/genética
Genes Bacterianos
Esporos Bacterianos/crescimento & desenvolvimento
Esporos Bacterianos/genética
[Mh] Termos MeSH secundário: Sequência Conservada
Deleção de Genes
Filogenia
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1302
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121106
[St] Status:MEDLINE
[do] DOI:10.1128/JB.01778-12


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[PMID]:22476275
[Au] Autor:Green CF; Davidson CS; Panlilio AL; Jensen PA; Jin Y; Gibbs SG; Scarpino PV
[Ad] Endereço:Science, Math, and Engineering Division, University of Cincinnati Clermont College, Batavia, Ohio, USA.
[Ti] Título:Effectiveness of selected surgical masks in arresting vegetative cells and endospores when worn by simulated contagious patients.
[So] Source:Infect Control Hosp Epidemiol;33(5):487-94, 2012 May.
[Is] ISSN:1559-6834
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The objective of this study was to quantify the effectiveness of selected surgical masks in arresting vegetative cells and endospores in an experimental model that simulated contagious patients. SETTING: Laboratory. METHODS: Five commercially available surgical masks were tested for their ability to arrest infectious agents. Surgical masks were placed over the nose and mouth of mannequin head forms (Simulaids adult model Brad CPR torso). The mannequins were retrofitted with a nebulizer attached to an automated breathing simulator calibrated to a tidal volume of 500 mL/breath and a breathing rate of 20 breaths/min, for a minute respiratory volume of 10 L/min. Aerosols of endospores or vegetative cells were generated with a modified microbiological research establishment-type 6-jet collision nebulizer, while air samples were taken with all-glass impinger (AGI-30) samplers downstream of the point source. All experiments were conducted in a horizontal bioaerosol chamber. RESULTS: Mean arrestance of bioaerosols by the surgical masks ranged from 48% to 68% when the masks were challenged with endospores and from 66% to 76% when they were challenged with vegetative cells. When the arrestance of endospores was evaluated, statistical differences were observed between some pairs, though not all, of the models evaluated. There were no statistically significant differences in arrestance observed between models of surgical masks challenged with vegetative cells. CONCLUSIONS: The arrestance of airborne vegetative cells and endospores by surgical masks worn by simulated contagious patients supports surgical mask use as one of the recommended cough etiquette interventions to limit the transmission of airborne infectious agents.
[Mh] Termos MeSH primário: Controle de Doenças Transmissíveis/métodos
Bactérias Formadoras de Endosporo
Manequins
Máscaras/normas
Roupa de Proteção/normas
[Mh] Termos MeSH secundário: Laboratórios
Estados Unidos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Em] Mês de entrada:1208
[Cu] Atualização por classe:150127
[Lr] Data última revisão:
150127
[Sb] Subgrupo de revista:IM; N
[Da] Data de entrada para processamento:120406
[St] Status:MEDLINE
[do] DOI:10.1086/665321


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[PMID]:21985154
[Au] Autor:Phelan RW; O'Halloran JA; Kennedy J; Morrissey JP; Dobson AD; O'Gara F; Barbosa TM
[Ad] Endereço:Department of Microbiology, University College Cork, Cork, Ireland.
[Ti] Título:Diversity and bioactive potential of endospore-forming bacteria cultured from the marine sponge Haliclona simulans.
[So] Source:J Appl Microbiol;112(1):65-78, 2012 Jan.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: Despite the frequent isolation of endospore-formers from marine sponges, little is known about the diversity and characterization of individual isolates. The main aims of this study were to isolate and characterize the spore-forming bacteria from the marine sponge Haliclona simulans and to examine their potential as a source for bioactive compounds. METHODS AND RESULTS: A bank of presumptive aerobic spore-forming bacteria was isolated from the marine sponge H. simulans. These represented c. 1% of the total culturable bacterial population. A subgroup of thirty isolates was characterized using morphological, phenotypical and phylogenetic analysis. A large diversity of endospore-forming bacteria was present, with the thirty isolates being distributed through a variety of Bacillus and Paenibacillus species. These included ubiquitous species, such as B. subtilis, B. pumilus, B. licheniformis and B. cereus group, as well as species that are typically associated with marine habitats, such as B. aquimaris, B. algicola and B. hwajinpoensis. Two strains carried the aiiA gene that encodes a lactonase known to be able to disrupt quorum-sensing mechanisms, and various isolates demonstrated protease activity and antimicrobial activity against different pathogenic indicator strains, including Clostridium perfringens, Bacillus cereus and Listeria monocytogenes. CONCLUSIONS: The marine sponge H. simulans harbours a diverse collection of endospore-forming bacteria, which produce proteases and antibiotics. This diversity appears to be overlooked by culture-dependent and culture-independent methods that do not specifically target sporeformers. SIGNIFICANCE AND IMPACT OF STUDY: Marine sponges are an as yet largely untapped and poorly understood source of endospore-forming bacterial diversity with potential biotechnological, biopharmaceutical and probiotic applications. These results also indicate the importance of combining different methodologies for the comprehensive characterization of complex microbial populations such as those found in marine sponges.
[Mh] Termos MeSH primário: Biodiversidade
Bactérias Formadoras de Endosporo/fisiologia
Haliclona/microbiologia
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/metabolismo
Anti-Infecciosos/farmacologia
Bactérias Formadoras de Endosporo/classificação
Bactérias Formadoras de Endosporo/efeitos dos fármacos
Bactérias Formadoras de Endosporo/genética
Bactérias Formadoras de Endosporo/isolamento & purificação
Bactérias Gram-Negativas/efeitos dos fármacos
Dados de Sequência Molecular
Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1204
[Cu] Atualização por classe:111214
[Lr] Data última revisão:
111214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111012
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-2672.2011.05173.x


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[PMID]:20336503
[Au] Autor:Bonadei M; Calvio C; Carbonera D; Galizzi A; Quattrini E; Balestrazzi A
[Ad] Endereço:Department of Genetics and Microbiology, University of Pavia, 27100 Pavia, Italy.
[Ti] Título:Spore-forming bacteria in soil cultivated with GM white poplars: isolation and characterization.
[So] Source:Folia Microbiol (Praha);55(1):39-46, 2010 Jan.
[Is] ISSN:1874-9356
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The impact of transgenic white poplars (Populus alba L. cv. 'Villafranca') was assessed on the soil aerobic spore-forming bacteria (SFB). The genetically modified poplars, expressing either the StSy gene for resveratrol production or the bar gene for herbicide tolerance, were cultivated in greenhouse. The occurrence of SFB was monitored in soil samples collected at eight different timepoints over a two-year period. The total culturable bacterial population of the StSy and bar trials underwent significant seasonal fluctuations in the range of 10(6)-2.5 x 10(8) CFU/g dry soil and of 10(4)-5 x 10(8) CFU/g dry soil, respectively. Changes occurred also within the culturable SFB population with size varying at 10(3)-5 x 10(4) CFU/g dry soil and 10(2)-2 x 10(5) CFU/g dry soil in the StSy and bar trials, respectively. No significant differences in the size of the total and SFB culturable populations were observed when comparing each transgenic line with the nontransformed control line while seasonal shifts of soil bacterial populations were evident in both trials. The culturable SFB fraction included three isolates (SFB-1, SFB-2 and SFB-3) classified by 16S rDNA sequence analysis as members of the Bacillus genus. According to the reported data, cultivation of both herbicide-resistant and resveratrol-producing GM white poplars did not affect the culturable SFB population at the soil level.
[Mh] Termos MeSH primário: Bactérias Formadoras de Endosporo/classificação
Bactérias Formadoras de Endosporo/isolamento & purificação
Plantas Geneticamente Modificadas/microbiologia
Populus/microbiologia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Aerobiose
Análise por Conglomerados
Contagem de Colônia Microbiana
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Bactérias Formadoras de Endosporo/fisiologia
Dados de Sequência Molecular
Filogenia
RNA Ribossômico 16S/genética
Estações do Ano
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100326
[St] Status:MEDLINE
[do] DOI:10.1007/s12223-010-0007-8



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