Base de dados : MEDLINE
Pesquisa : B03.300.390 [Categoria DeCS]
Referências encontradas : 123 [refinar]
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[PMID]:26982366
[Au] Autor:Kumar P; Monin L; Castillo P; Elsegeiny W; Horne W; Eddens T; Vikram A; Good M; Schoenborn AA; Bibby K; Montelaro RC; Metzger DW; Gulati AS; Kolls JK
[Ad] Endereço:Richard King Mellon Foundation Institute for Pediatric Research, Children's Hospital of Pittsburgh of UPMC, University of Pittsburgh, Pittsburgh, PA 15224, USA.
[Ti] Título:Intestinal Interleukin-17 Receptor Signaling Mediates Reciprocal Control of the Gut Microbiota and Autoimmune Inflammation.
[So] Source:Immunity;44(3):659-671, 2016 Mar 15.
[Is] ISSN:1097-4180
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Interleukin-17 (IL-17) and IL-17 receptor (IL-17R) signaling are essential for regulating mucosal host defense against many invading pathogens. Commensal bacteria, especially segmented filamentous bacteria (SFB), are a crucial factor that drives T helper 17 (Th17) cell development in the gastrointestinal tract. In this study, we demonstrate that Th17 cells controlled SFB burden. Disruption of IL-17R signaling in the enteric epithelium resulted in SFB dysbiosis due to reduced expression of α-defensins, Pigr, and Nox1. When subjected to experimental autoimmune encephalomyelitis, IL-17R-signaling-deficient mice demonstrated earlier disease onset and worsened severity that was associated with increased intestinal Csf2 expression and elevated systemic GM-CSF cytokine concentrations. Conditional deletion of IL-17R in the enteric epithelium demonstrated that there was a reciprocal relationship between the gut microbiota and enteric IL-17R signaling that controlled dysbiosis, constrained Th17 cell development, and regulated the susceptibility to autoimmune inflammation.
[Mh] Termos MeSH primário: Encefalomielite Autoimune Experimental/imunologia
Infecções por Bactérias Gram-Positivas/imunologia
Bactérias Gram-Positivas Formadoras de Endosporo/imunologia
Intestinos/fisiologia
Receptores de Interleucina-17/metabolismo
Células Th17/imunologia
[Mh] Termos MeSH secundário: Animais
Disbiose/genética
Fator Estimulador de Colônias de Granulócitos e Macrófagos/sangue
Fator Estimulador de Colônias de Granulócitos e Macrófagos/genética
Fator Estimulador de Colônias de Granulócitos e Macrófagos/metabolismo
Interações Hospedeiro-Patógeno
Imunidade nas Mucosas/genética
Interleucina-17/metabolismo
Intestinos/microbiologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Microbiota
NADH NADPH Oxirredutases/genética
NADH NADPH Oxirredutases/metabolismo
NADPH Oxidase 1
Receptores de Superfície Celular/genética
Receptores de Superfície Celular/metabolismo
Receptores de Interleucina-17/genética
Transdução de Sinais/genética
Células Th17/microbiologia
alfa-Defensinas/genética
alfa-Defensinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Interleukin-17); 0 (Oscar protein, mouse); 0 (Receptors, Cell Surface); 0 (Receptors, Interleukin-17); 0 (alpha-Defensins); 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor); EC 1.6.- (NADH, NADPH Oxidoreductases); EC 1.6.3.- (NADPH Oxidase 1); EC 1.6.3.- (NOX1 protein, mouse)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160317
[St] Status:MEDLINE


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[PMID]:25858227
[Au] Autor:Farkas AM; Panea C; Goto Y; Nakato G; Galan-Diez M; Narushima S; Honda K; Ivanov II
[Ad] Endereço:Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032, United States.
[Ti] Título:Induction of Th17 cells by segmented filamentous bacteria in the murine intestine.
[So] Source:J Immunol Methods;421:104-11, 2015 Jun.
[Is] ISSN:1872-7905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Segmented filamentous bacteria (SFB) are Gram-positive, anaerobic, spore-forming commensals that reside in the gut of many animal species. Described more than forty years ago, SFB have recently gained interest due to their unique ability to modulate the host immune system through induction of IgA and Th17 cells. Here, we describe a collection of methods to detect and quantify SFB and SFB adhesion in intestinal mucosa, as well as SFB-specific CD4 T cells in the lamina propria. In addition, we describe methods for purification of SFB from fecal material of SFB-monoassociated gnotobiotic mice. Using these methods we examine the kinetics of SFB colonization and Th17 cell induction. We also show that SFB colonize unevenly the intestinal mucosa and that SFB adherence occurs predominantly in the terminal ileum and correlates with an increased proportion of SFB-specific Th17 cells.
[Mh] Termos MeSH primário: Infecções por Bactérias Gram-Positivas/imunologia
Bactérias Gram-Positivas Formadoras de Endosporo/imunologia
Mucosa Intestinal/imunologia
Mucosa Intestinal/microbiologia
Células Th17/imunologia
[Mh] Termos MeSH secundário: Animais
Aderência Bacteriana/imunologia
Fezes/microbiologia
Camundongos
Camundongos Endogâmicos C57BL
Microbiota/imunologia
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1508
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150411
[St] Status:MEDLINE


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[PMID]:25227686
[Au] Autor:D Santos AF; Pacheco CA; Valle RD; Seldin L; D Santos AL
[Ad] Endereço:Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
[Ti] Título:Enzymes produced by halotolerant spore-forming gram-positive bacterial strains isolated from a resting habitat (Restinga de Jurubatiba) in Rio de Janeiro, Brazil: focus on proteases.
[So] Source:Appl Biochem Biotechnol;174(8):2748-61, 2014 Dec.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The screening for hydrolases-producing, halotolerant, and spore-forming gram-positive bacteria from the root, rhizosphere, and non-rhizosphere soil of Blutaparon portulacoides, a plant found in the Restinga de Jurubatiba located at the northern region of Rio de Janeiro State, Brazil, resulted in the isolation of 22 strains. These strains were identified as Halobacillus blutaparonensis (n = 2), Oceanobacillus picturae (n = 5), and Oceanobacillus iheyensis (n = 15), and all showed the ability to produce different extracellular enzymes. A total of 20 isolates (90.9 %) showed activity for protease, 5 (22.7 %) for phytase, 3 (13.6 %) for cellulase, and 2 (9.1 %) for amylase. Some bacterial strains were capable of producing three (13.6 %) or two (9.1 %) distinct hydrolytic enzymes. However, no bacterial strain with ability to produce esterase and DNase was observed. The isolate designated M9, belonging to the species H. blutaparonensis, was the best producer of protease and also yielded amylase and phytase. This strain was chosen for further studies regarding its protease activity. The M9 strain produced similar amounts of protease when grown either without or with different NaCl concentrations (from 0.5 to 10 %). A simple inspection of the cell-free culture supernatant by gelatin-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed the presence of three major alkaline proteases of 40, 50, and 70 kDa, which were fully inhibited by phenylmethylsulfonyl fluoride (PMSF) and tosyl-L-phenylalanine chloromethyl ketone (TPCK) (two classical serine protease inhibitors). The secreted proteases were detected in a wide range of temperature (from 4 to 45 °C) and their hydrolytic activities were stimulated by NaCl (up to 10 %). The serine proteases produced by the M9 strain cleaved gelatin, casein, albumin, and hemoglobin, however, in different extensions. Collectively, these results suggest the potential use of the M9 strain in biotechnological and/or industrial processes.
[Mh] Termos MeSH primário: Amaranthaceae/microbiologia
Proteínas de Bactérias/biossíntese
Ecossistema
Bactérias Gram-Positivas Formadoras de Endosporo
Peptídeo Hidrolases/biossíntese
Rizoma/microbiologia
[Mh] Termos MeSH secundário: Bactérias Gram-Positivas Formadoras de Endosporo/enzimologia
Bactérias Gram-Positivas Formadoras de Endosporo/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:141121
[Lr] Data última revisão:
141121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140918
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-014-1223-5


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[PMID]:24325907
[Au] Autor:Remely M; Aumueller E; Merold C; Dworzak S; Hippe B; Zanner J; Pointner A; Brath H; Haslberger AG
[Ad] Endereço:Department of Nutritional Sciences, University Vienna, Vienna, Austria.
[Ti] Título:Effects of short chain fatty acid producing bacteria on epigenetic regulation of FFAR3 in type 2 diabetes and obesity.
[So] Source:Gene;537(1):85-92, 2014 Mar 01.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The human gut microbiota and microbial influences on lipid and glucose metabolism, satiety, and chronic low-grade inflammation are known to be involved in metabolic syndrome. Fermentation end products, especially short chain fatty acids, are believed to engage the epigenetic regulation of inflammatory reactions via FFARs (free fatty acid receptor) and other short chain fatty acid receptors. We studied a potential interaction of the microbiota with epigenetic regulation in obese and type 2 diabetes patients compared to a lean control group over a four month intervention period. Intervention comprised a GLP-1 agonist (glucagon-like peptide 1) for type 2 diabetics and nutritional counseling for both intervention groups. Microbiota was analyzed for abundance, butyryl-CoA:acetate CoA-transferase gene and for diversity by polymerase chain reaction and 454 high-throughput sequencing. Epigenetic methylation of the promoter region of FFAR3 and LINE1 (long interspersed nuclear element 1) was analyzed using bisulfite conversion and pyrosequencing. The diversity of the microbiota as well as the abundance of Faecalibacterium prausnitzii were significantly lower in obese and type 2 diabetic patients compared to lean individuals. Results from Clostridium cluster IV and Clostridium cluster XIVa showed a decreasing trend in type 2 diabetics in comparison to the butyryl-CoA:acetate CoA-transferase gene and according to melt curve analysis. During intervention no significant changes were observed in either intervention group. The analysis of five CpGs in the promoter region of FFAR3 showed a significant lower methylation in obese and type 2 diabetics with an increase in obese patients over the intervention period. These results disclosed a significant correlation between a higher body mass index and lower methylation of FFAR3. LINE-1, a marker of global methylation, indicated no significant differences between the three groups or the time points, although methylation of type 2 diabetics tended to increase over time. Our results provide evidence that a different composition of gut microbiota in obesity and type 2 diabetes affect the epigenetic regulation of genes. Interactions between the microbiota and epigenetic regulation may involve not only short chain fatty acids binding to FFARs. Therefore dietary interventions influencing microbial composition may be considered as an option in the engagement against metabolic syndrome.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 2/genética
Epigênese Genética
Ácidos Graxos Voláteis/metabolismo
Trato Gastrointestinal/microbiologia
Obesidade/genética
Receptores Acoplados a Proteínas-G/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Biodiversidade
Índice de Massa Corporal
Estudos de Casos e Controles
Coenzima A-Transferases/genética
Metilação de DNA
Diabetes Mellitus Tipo 2/tratamento farmacológico
Diabetes Mellitus Tipo 2/microbiologia
Fezes/microbiologia
Comportamento Alimentar
Feminino
Peptídeo 1 Semelhante ao Glucagon/agonistas
Peptídeo 1 Semelhante ao Glucagon/análogos & derivados
Peptídeo 1 Semelhante ao Glucagon/uso terapêutico
Bactérias Gram-Positivas/fisiologia
Bactérias Gram-Positivas Formadoras de Endosporo/genética
Seres Humanos
Liraglutida
Elementos Nucleotídeos Longos e Dispersos
Masculino
Microbiota/fisiologia
Meia-Idade
Obesidade/microbiologia
Regiões Promotoras Genéticas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (FFAR3 protein, human); 0 (Fatty Acids, Volatile); 0 (Receptors, G-Protein-Coupled); 839I73S42A (Liraglutide); 89750-14-1 (Glucagon-Like Peptide 1); EC 2.8.3.- (Coenzyme A-Transferases); EC 2.8.3.- (butyryl-CoA acetoacetate CoA transferase)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131212
[St] Status:MEDLINE


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[PMID]:24118059
[Au] Autor:Blumer-Schuette SE; Brown SD; Sander KB; Bayer EA; Kataeva I; Zurawski JV; Conway JM; Adams MW; Kelly RM
[Ad] Endereço:Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, USA; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
[Ti] Título:Thermophilic lignocellulose deconstruction.
[So] Source:FEMS Microbiol Rev;38(3):393-448, 2014 May.
[Is] ISSN:1574-6976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Thermophilic microorganisms are attractive candidates for conversion of lignocellulose to biofuels because they produce robust, effective, carbohydrate-degrading enzymes and survive under harsh bioprocessing conditions that reflect their natural biotopes. However, no naturally occurring thermophile is known that can convert plant biomass into a liquid biofuel at rates, yields and titers that meet current bioprocessing and economic targets. Meeting those targets requires either metabolically engineering solventogenic thermophiles with additional biomass-deconstruction enzymes or engineering plant biomass degraders to produce a liquid biofuel. Thermostable enzymes from microorganisms isolated from diverse environments can serve as genetic reservoirs for both efforts. Because of the sheer number of enzymes that are required to hydrolyze plant biomass to fermentable oligosaccharides, the latter strategy appears to be the preferred route and thus has received the most attention to date. Thermophilic plant biomass degraders fall into one of two categories: cellulosomal (i.e. multienzyme complexes) and noncellulosomal (i.e. 'free' enzyme systems). Plant-biomass-deconstructing thermophilic bacteria from the genera Clostridium (cellulosomal) and Caldicellulosiruptor (noncellulosomal), which have potential as metabolic engineering platforms for producing biofuels, are compared and contrasted from a systems biology perspective.
[Mh] Termos MeSH primário: Biocombustíveis
Bactérias Gram-Positivas Formadoras de Endosporo/enzimologia
Lignina/metabolismo
[Mh] Termos MeSH secundário: Celulossomas/genética
Celulossomas/metabolismo
Bactérias Gram-Positivas Formadoras de Endosporo/classificação
Bactérias Gram-Positivas Formadoras de Endosporo/genética
Temperatura Alta
Dados de Sequência Molecular
Plantas/metabolismo
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; REVIEW
[Nm] Nome de substância:
0 (Biofuels); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:140505
[Lr] Data última revisão:
140505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131015
[St] Status:MEDLINE
[do] DOI:10.1111/1574-6976.12044


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[PMID]:24249300
[Au] Autor:Wunderlin T; Junier T; Roussel-Delif L; Jeanneret N; Junier P
[Ad] Endereço:Laboratory of Microbiology, Institute of Biology, University of Neuchâtel, Neuchâtel, CH-2000, Switzerland.
[Ti] Título:Stage 0 sporulation gene A as a molecular marker to study diversity of endospore-forming Firmicutes.
[So] Source:Environ Microbiol Rep;5(6):911-24, 2013 Dec.
[Is] ISSN:1758-2229
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we developed and validated a culture-independent method for diversity surveys to specifically detect endospore-forming Firmicutes. The global transcription regulator of sporulation (spo0A) was identified as a gene marker for endospore-forming Firmicutes. To enable phylogenetic classification, we designed a set of primers amplifying a 602 bp fragment of spo0A that we evaluated in pure cultures and environmental samples. The amplification was positive for 35 strains from 11 genera, yet negative for strains from Alicyclobacillus and Sulfobacillus. We also evaluated various DNA extraction methods because endospores often result in reduced yields. Our results demonstrate that procedures utilizing increased physical force improve DNA extraction. An optimized DNA extraction method on biomass pre-extracted from the environmental sample source (indirect DNA extraction) followed by amplification with the aforementioned primers for spo0A was then tested in sediments from two different sources. Specifically, we validated our culture-independent diversity survey methodology on a set of 8338 environmental spo0A sequences obtained from the sediments of Lakes Geneva (Switzerland) and Baikal (Russia). The phylogenetic affiliation of the environmental sequences revealed a substantial number of new clades within endospore-formers. This novel culture-independent approach provides a significant experimental improvement that enables exploration of the diversity of endospore-forming Firmicutes.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Bactérias Gram-Positivas Formadoras de Endosporo/genética
Esporos Bacterianos/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Biodiversidade
DNA Bacteriano/genética
Genes Bacterianos
Marcadores Genéticos
Bactérias Gram-Positivas Formadoras de Endosporo/classificação
Bactérias Gram-Positivas Formadoras de Endosporo/metabolismo
Dados de Sequência Molecular
Técnicas de Amplificação de Ácido Nucleico
Filogenia
RNA Ribossômico 16S/genética
Alinhamento de Sequência
Análise de Sequência de DNA
Esporos Bacterianos/crescimento & desenvolvimento
Esporos Bacterianos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Genetic Markers); 0 (RNA, Ribosomal, 16S); 0 (Spo0A protein, Bacillus subtilis); 0 (Transcription Factors)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:131119
[Lr] Data última revisão:
131119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131120
[St] Status:MEDLINE
[do] DOI:10.1111/1758-2229.12094


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[PMID]:24199681
[Au] Autor:Zhang W; Fang D; Zhang T; Zhou L; Jiang B; Mu W
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Jiangnan University , 214122 Wuxi, People's Republic of China.
[Ti] Título:Characterization of a metal-dependent D-psicose 3-epimerase from a novel strain, Desmospora sp. 8437.
[So] Source:J Agric Food Chem;61(47):11468-76, 2013 Nov 27.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The rare sugar d-psicose is an ideal sucrose substitute for food products, due to having 70% of the relative sweetness but 0.3% of the energy of sucrose. It also shows important physiological functions. d-Tagatose 3-epimerase (DTEase) family enzymes can produce d-psicose from d-fructose. In this paper, a new member of the DTEase family of enzymes was characterized from Desmospora sp. 8437 (GenBank accession no. WP_009711885 ) and was named Desmospora sp. d-psicose 3-epimerase (DPEase) due to its highest substrate specificity toward d-psicose. Desmospora sp. DPEase was strictly metal-dependent and displayed maximum activity in the presence of Co(2+). The optimum pH and temperature were 7.5 and 60 °C, respectively. The enzyme was relatively thermostable below 50 °C, but easily lost initial activity when preincubated at 60 °C. The thermostability property was almost not affected by the addition of Co(2+). Desmospora sp. DPEase had relatively high catalysis efficiency for the substrates d-psicose and d-fructose, which were measured to be 327 and 116 mM(-1) min(-1), respectively. The equilibrium ratio between d-psicose and d-fructose of Desmospora sp. DPEase was 30:70. The enzyme could produce 142.5 g/L d-psicose from 500 g/L of d-fructose, suggesting that the enzyme is a potential d-psicose producer for industrial production.
[Mh] Termos MeSH primário: Frutose/metabolismo
Bactérias Gram-Positivas Formadoras de Endosporo/enzimologia
Metais/metabolismo
Racemases e Epimerases/genética
Racemases e Epimerases/metabolismo
[Mh] Termos MeSH secundário: Clonagem Molecular
Estabilidade Enzimática
Bactérias Gram-Positivas Formadoras de Endosporo/genética
Concentração de Íons de Hidrogênio
Cinética
Filogenia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Metals); 23140-52-5 (psicose); 30237-26-4 (Fructose); EC 5.1.- (Racemases and Epimerases)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:131127
[Lr] Data última revisão:
131127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131109
[St] Status:MEDLINE
[do] DOI:10.1021/jf4035817


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[PMID]:23811505
[Au] Autor:Bueche M; Wunderlin T; Roussel-Delif L; Junier T; Sauvain L; Jeanneret N; Junier P
[Ad] Endereço:Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Neuchatel, Switzerland.
[Ti] Título:Quantification of endospore-forming firmicutes by quantitative PCR with the functional gene spo0A.
[So] Source:Appl Environ Microbiol;79(17):5302-12, 2013 Sep.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial endospores are highly specialized cellular forms that allow endospore-forming Firmicutes (EFF) to tolerate harsh environmental conditions. EFF are considered ubiquitous in natural environments, in particular, those subjected to stress conditions. In addition to natural habitats, EFF are often the cause of contamination problems in anthropogenic environments, such as industrial production plants or hospitals. It is therefore desirable to assess their prevalence in environmental and industrial fields. To this end, a high-sensitivity detection method is still needed. The aim of this study was to develop and evaluate an approach based on quantitative PCR (qPCR). For this, the suitability of functional genes specific for and common to all EFF were evaluated. Seven genes were considered, but only spo0A was retained to identify conserved regions for qPCR primer design. An approach based on multivariate analysis was developed for primer design. Two primer sets were obtained and evaluated with 16 pure cultures, including representatives of the genera Bacillus, Paenibacillus, Brevibacillus, Geobacillus, Alicyclobacillus, Sulfobacillus, Clostridium, and Desulfotomaculum, as well as with environmental samples. The primer sets developed gave a reliable quantification when tested on laboratory strains, with the exception of Sulfobacillus and Desulfotomaculum. A test using sediment samples with a diverse EFF community also gave a reliable quantification compared to 16S rRNA gene pyrosequencing. A detection limit of about 10(4) cells (or spores) per gram of initial material was calculated, indicating this method has a promising potential for the detection of EFF over a wide range of applications.
[Mh] Termos MeSH primário: Carga Bacteriana/métodos
Proteínas de Bactérias/genética
Bactérias Gram-Positivas Formadoras de Endosporo/genética
Bactérias Gram-Positivas Formadoras de Endosporo/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Primers do DNA/genética
Sensibilidade e Especificidade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA Primers)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130702
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01376-13


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[PMID]:23035569
[Au] Autor:Gumerov VM; Mardanov AV; Kolosov PM; Ravin NV
[Ti] Título:[Isolation and functional characterization of lipase from the thermophilic alkali-tolerant bacterium Thermosyntropha lipolytica].
[So] Source:Prikl Biokhim Mikrobiol;48(4):376-82, 2012 Jul-Aug.
[Is] ISSN:0555-1099
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally characterized. The lipase exhibited hydrolytic activity toward para-nitrophenyl esters of various chain lengths, as well as triglycerides, including vegetable oils. The optimal reaction conditions were achieved at temperatures from 70 to 80 degrees C and pH 8.0. Enzyme saved more than 80% of its activity in the presence of 10% methanol. This new thermostable lipase may be a promising biocatalyst for organic synthesis; it may find application in the food and detergent industry and biodiesel production.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Genoma Bacteriano
Bactérias Gram-Positivas Formadoras de Endosporo/enzimologia
Lipase/genética
Óleos Vegetais/metabolismo
[Mh] Termos MeSH secundário: Álcalis
Sequência de Aminoácidos
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/metabolismo
Clonagem Molecular
Escherichia coli
Bactérias Gram-Positivas Formadoras de Endosporo/genética
Temperatura Alta
Concentração de Íons de Hidrogênio
Lipase/isolamento & purificação
Lipase/metabolismo
Lipólise
Dados de Sequência Molecular
Nitrofenóis
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkalies); 0 (Bacterial Proteins); 0 (Nitrophenols); 0 (Plant Oils); 0 (Recombinant Proteins); 0 (Triglycerides); 2395-99-5 (4-nitrophenyl); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1210
[Cu] Atualização por classe:121005
[Lr] Data última revisão:
121005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121006
[St] Status:MEDLINE


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[PMID]:23009985
[Au] Autor:Khuda F; Iqbal Z; Zakiullah; Khan A; Nasir F
[Ad] Endereço:Department of Pharmacy, University of Peshawar, Peshawar, Pakistan. fazli_khuda2008@yahoo.com
[Ti] Título:Antimicrobial and anti-inflammatory activities of leaf extract of Valeriana wallichii DC.
[So] Source:Pak J Pharm Sci;25(4):715-9, 2012 Oct.
[Is] ISSN:1011-601X
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:Valeriana wallichii DC (Valerianaceae) is one of the most widely used traditional remedies for various complications associated with nervous system and digestion. No antimicrobial and anti-inflammatory studies have so far been carried out on the aerial parts of the plant. The present work was focused to evaluate the antimicrobial (antifungal and antibacterial) and anti-inflammatory properties of V. wallichii using reported methods. Chloroform fraction (VW-2) and hexane fraction (VW-3) exhibited significant activity against S. aureus and B. subtilus, respectively. The chloroform fraction (VW-2) showed significant activity against S. aureus with 0.27 mg/ml MIC, where 0.31 mg/ml MIC was deduced for VW-3 fraction against B. subtilus. VW-3 fraction was also found to be the most potent inhibitor of M. canis, showing 70% inhibition with an MIC value of 0.19 mg/ml. Considerable inhibitory activity was also observed for VW-2 and water fraction (VW-6) against M. canis and A. flavus. A remarkable anti-inflammatory like activity was observed for the crude extract at a dose of 200 mg/kg at all observed durations. Other doses of the sample also showed excellent activity. Looking to these results it may be concluded that V. wallichii may be a potential source for activity guided isolation of natural products with antimicrobial and anti-inflammatory-like properties.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Anti-Inflamatórios/farmacologia
Antifúngicos/farmacologia
Edema/prevenção & controle
Bactérias Gram-Positivas Formadoras de Endosporo/efeitos dos fármacos
Fungos Mitospóricos/efeitos dos fármacos
Valeriana
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/isolamento & purificação
Anti-Inflamatórios/química
Anti-Inflamatórios/isolamento & purificação
Antifúngicos/química
Antifúngicos/isolamento & purificação
Aspergillus flavus/efeitos dos fármacos
Aspergillus flavus/crescimento & desenvolvimento
Bacillus subtilis/efeitos dos fármacos
Bacillus subtilis/crescimento & desenvolvimento
Carragenina
Clorofórmio/química
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Edema/induzido quimicamente
Bactérias Gram-Positivas Formadoras de Endosporo/crescimento & desenvolvimento
Hexanos/química
Masculino
Testes de Sensibilidade Microbiana
Microsporum/efeitos dos fármacos
Microsporum/crescimento & desenvolvimento
Fungos Mitospóricos/crescimento & desenvolvimento
Folhas de Planta
Ratos
Ratos Wistar
Solventes/química
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/crescimento & desenvolvimento
Valeriana/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Inflammatory Agents); 0 (Antifungal Agents); 0 (Hexanes); 0 (Solvents); 7V31YC746X (Chloroform); 9000-07-1 (Carrageenan)
[Em] Mês de entrada:1212
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120927
[St] Status:MEDLINE



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