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Pesquisa : B03.300.390.400.158 [Categoria DeCS]
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  1 / 916 MEDLINE  
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[PMID]:29458475
[Au] Autor:Gan L; Zhang H; Long X; Tian J; Wang Z; Zhang Y; Dai Y; Tian Y
[Ad] Endereço:1​Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
[Ti] Título:Ornithinibacillus salinisoli sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.
[So] Source:Int J Syst Evol Microbiol;68(3):769-775, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A taxonomic study was performed on strain LCB256 , which was isolated from a saline-alkali soil sample taken from northwestern China. Cells of strain LCB256 were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256 was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823 (97.8 %) and Ornithinibacillus bavariensis WSBC 24001 (97.2 %). The peptidoglycan amino acid type was found to be A4ß and the major respiratory quinone was determined to be MK-7. The polar lipid profile of strain LCB256 contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids. The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The G+C content of genomic DNA was 39.3 mol%. DNA-DNA relatedness values between strain LCB256 and Ornithinibacillus halophilus KCTC 13822 and Oceanobacillus limi KCTC 13823 were 46.2 and 34.8 %, respectively. Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp. nov. is proposed. The type strain is LCB256 (=CGMCC 1.15809 =KCTC 33862 ).
[Mh] Termos MeSH primário: Bacillaceae/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Álcalis
Bacillaceae/genética
Bacillaceae/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Salinidade
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002580


  2 / 916 MEDLINE  
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[PMID]:29172504
[Au] Autor:Wu Q; Lin J; Cui K; Du R; Zhu Y; Xu Y
[Ad] Endereço:The Key Laboratory of Industrial Biotechnology, Ministry of Education, State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Biotechnology, Jiangnan University , Wuxi, Jiangsu 214122, China.
[Ti] Título:Effect of Microbial Interaction on Urea Metabolism in Chinese Liquor Fermentation.
[So] Source:J Agric Food Chem;65(50):11133-11139, 2017 Dec 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Urea is the primary precursor of the carcinogen ethyl carbamate in fermented foods. Understanding urea metabolism is important for controlling ethyl carbamate production. Using Chinese liquor as a model system, we used metatranscriptome analysis to investigate urea metabolism in spontaneous food fermentation processes. Saccharomyces cerevisiae was dominant in gene transcription for urea biosynthesis and degradation. Lysinibacillus sphaericus was dominant for urea degradation. S. cerevisiae degraded 18% and L. sphaericus degraded 13% of urea in their corresponding single cultures, whereas they degraded 56% of urea in coculture after 12 h. Compared to single cultures, transcription of CAR1, DAL2, and argA, which are related to urea biosynthesis, decreased by 51, 36, and 69% in coculture, respectively. Transcription of DUR1 and ureA, which are related to urea degradation, increased by 227 and 70%, respectively. Thus, coexistence of the two strains promoted degradation of urea via transcriptional regulation of genes related to urea metabolism.
[Mh] Termos MeSH primário: Bacillaceae/metabolismo
Saccharomyces cerevisiae/metabolismo
Ureia/metabolismo
Vinho/microbiologia
[Mh] Termos MeSH secundário: Bacillaceae/genética
Fermentação
Saccharomyces cerevisiae/genética
Sorghum/metabolismo
Sorghum/microbiologia
Vinho/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8W8T17847W (Urea)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04099


  3 / 916 MEDLINE  
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[PMID]:28460092
[Au] Autor:Kumar Mondal A; Kumar J; Pandey R; Gupta S; Kumar M; Bansal G; Mukerji M; Dash D; Singh Chauhan N
[Ad] Endereço:G.N.Ramachandran Knowledge Centre for Genome Informatics, CSIR-Institute of Genomics and Integrative Biology, New Delhi, India.
[Ti] Título:Comparative Genomics of Host-Symbiont and Free-Living Oceanobacillus Species.
[So] Source:Genome Biol Evol;9(5):1175-1182, 2017 05 01.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Survival in a given environment requires specific functions, so genomic variation is anticipated within in individual taxonomic groups that exhibit a large diversity in lifestyles. In this study, we sequence and assemble the genome of Oceanobacillus faecalis strain HM6, a resident of the human gut. Using the genus Oceanobacillus and the HM6 draft genome sequence, we explore the functional requirements for survival in a symbiotic arrangement within the human gut, in contrast to free living in the environment. Comparative genomics of seven available Oceanobacillus complete genomes highlight a genomically heterogeneous group. Our analysis did not find strict phylogenetic separation between free-living and host-symbiont Oceanobacillus members. By comparing functional gene content between host-associated and free-living species, we identified candidate genes that are potentially involved in symbiotic lifestyles, including phosphotransferase genes, transporters and two component response regulators. This study summarizes genomic and phylogenetic differences in the Oceanobacillus genus. Additionally, we highlight functions that may be key for survival in the human gut community.
[Mh] Termos MeSH primário: Bacillaceae/genética
Bacillaceae/fisiologia
[Mh] Termos MeSH secundário: Bacillaceae/classificação
Ecossistema
Microbioma Gastrointestinal
Genoma Bacteriano
Seres Humanos
Filogenia
Análise de Sequência de DNA
Simbiose
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evx076


  4 / 916 MEDLINE  
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[PMID]:28923247
[Au] Autor:Chen YH; Chiu CC; Hung SW; Liu JY; Wang YC; Lv Q; Hsu CC; Huang YW; Huang WC; Chuang HL; Chen TH
[Ad] Endereço:Graduate Institute of Veterinary Pathobiology, National Chung Hsin University, Taichung 402, Taiwan.
[Ti] Título:Effects of plant- and animal-based high-fat diets on lipid storage and distribution in environmental bacteria-colonized gnotobiotic mice.
[So] Source:Biochem Biophys Res Commun;493(2):1075-1081, 2017 Nov 18.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Different edible oils such as lard and soybean oil have been reported to interact with the gut microbiota, affecting host lipid metabolism. However, whether bacteria derived from the environment influence host lipid metabolism remains unclear. This study aimed to clarify the roles of environmental bacteria in host lipid storage and distribution with various edible oils. Gnotobiotic C57BL/6JNarl mice were inoculated with Lysinibacillus xylanilyticus and Paenibacillus azoreducens and then fed either a normal diet (LabDiet 5010, control group) or a diet containing 60% lard (L-group) or soybean oil (S-group) for 18 months. Interestingly, the S-group accumulated massive amounts of white adipose tissue compared to the L- and control groups, while the L-group displayed more hepatic steatosis and fatty droplets than the other groups. The expression of fatty acid synthase (FAS), hydroxymethylglutaryl-coenzyme A reductase (HMGCR), sterol regulatory element-binding protein 2 (SREBP2), and peroxisome proliferator-activated receptor gamma (PPARγ) in the livers of the L-group were markedly elevated compared to the S-group. FAS and PPARγ protein levels were also markedly elevated. However, there were no differences in the expression of the pro-inflammatory cytokines interleukin-6 and tumor necrosis factor-α between the groups. Our results suggest that environmental bacteria may affect host hepatic inflammation and lipid distribution in the presence of high-fat diets, with different effects depending on the fat type consumed.
[Mh] Termos MeSH primário: Dieta Hiperlipídica/efeitos adversos
Fígado Gorduroso/metabolismo
Fígado Gorduroso/microbiologia
Metabolismo dos Lipídeos
Fígado/metabolismo
Fígado/microbiologia
[Mh] Termos MeSH secundário: Animais
Bacillaceae/fisiologia
Gorduras na Dieta/efeitos adversos
Gorduras na Dieta/metabolismo
Fígado Gorduroso/patologia
Fígado/patologia
Masculino
Camundongos Endogâmicos C57BL
Paenibacillus/fisiologia
Óleo de Soja/efeitos adversos
Óleo de Soja/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fats); 8001-22-7 (Soybean Oil); SI6O3IW77Z (lard)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


  5 / 916 MEDLINE  
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[PMID]:28905696
[Au] Autor:Hirota K; Nishita M; Matsuyama H; Yumoto I
[Ad] Endereço:1​Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8571, Japan.
[Ti] Título:Paralkalibacillus indicireducens gen., nov., sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing.
[So] Source:Int J Syst Evol Microbiol;67(10):4050-4056, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Obligately alkaliphilic, indigo-reducing strains, designated Bps-1 , Bps-2 and Bps-3, were isolated from an indigo fermentation liquor used for dyeing, which was produced from sukumo (composted Polygonum indigo leaves) obtained from a craft centre in Data City, Hokkaido, Japan, by using medium containing cellulase-treated sukumo. The 16S rRNA gene sequence phylogeny suggested that Bps-1 has a distinctive position among the alkaliphilic species of the genus Bacillus, with its closest neighbours being Bacillus pseudofirmus DSM 8715 , Bacillus lindianensis DSM 26864 and Bacillus alcalophilus DSM 485 (96.1, 95.8 and 95.5 % 16S rRNA gene sequence similarities, respectively). The 16S rRNA sequence of strain Bps-1 was identical to those of strains Bps-2 and Bps-3. Cells of the novel isolate were Gram-stain-positive and were facultatively anaerobic straight rods that were motile by means of a pair of flagella (subpolar and centre sides). Spherical endospores were formed in the terminal position. Strain Bps-1 grew between 18 and 40 °C with optimum growth at 33 °C. The isolate grew in the pH range 8‒11, with optimum growth at pH 9‒10. The isoprenoid quinone detected was menaquinone-7 (MK-7), and the DNA G+C content was 40.3 %. The whole-cell fatty acid profile (>10 %) mainly consisted of anteiso-C15 : 0, iso-C15 : 0 and C16 : 0. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, the isolates represent a novel species of a novel genus, for which the name Paralkalibacillus indicireducens gen. nov., sp. nov. is proposed. The type strain of this species is Bps-1 (JCM 31808 =NCIMB 15080 ), with strains Bps-2 and Bps-3 representing additional strains of the species.
[Mh] Termos MeSH primário: Bacillaceae/classificação
Fermentação
Índigo Carmim
Filogenia
[Mh] Termos MeSH secundário: Bacillaceae/genética
Bacillaceae/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
Corantes
DNA Bacteriano/genética
Ácidos Graxos/química
Japão
Hibridização de Ácido Nucleico
Polygonum
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7); D3741U8K7L (Indigo Carmine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002248


  6 / 916 MEDLINE  
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[PMID]:28895514
[Au] Autor:Guo LY; Wang NN; Wang XQ; Chen GJ; Du ZJ
[Ad] Endereço:1​State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
[Ti] Título:Lentibacillus sediminis sp. nov., isolated from a marine saltern.
[So] Source:Int J Syst Evol Microbiol;67(10):3946-3950, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel, Gram-stain-positive, moderately halophilic, endospore-forming, motile, facultatively anaerobic and rod-shaped strain, designated 0W14 , was isolated from a marine saltern of Wendeng, China. Optimal growth occurred at 37 °C, pH 7.5 and with 6.0 % (w/v) NaCl. MK-7 was the sole respiratory quinone and the peptidoglycan type of 0W14 was A4ßl-Orn-d-Glu. The major cellular fatty acid (>10.0 %) in strain 0W14 was anteiso-C15 : 0. The polar lipid profile of strain 0W14 consisted of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and four unknown phospholipids. The genomic DNA G+C content of the strain was 44.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 0W14 forms a phylogenetic lineage with members of the genus Lentibacillus within the family Bacillaceae. Based on data from the current polyphasic study, the isolate is proposed to represent a novel species of genus Lentibacillus, for which the name Lentibacillus sediminis sp. nov. is proposed. The type strain is 0W14 (=KCTC 33835 =MCCC 1H00171 ).
[Mh] Termos MeSH primário: Bacillaceae/classificação
Filogenia
Água do Mar/microbiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Bacillaceae/genética
Bacillaceae/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Salinidade
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002228


  7 / 916 MEDLINE  
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[PMID]:28857029
[Au] Autor:Li J; Liu J; Shen Y; Ni J
[Ad] Endereço:State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
[Ti] Título:Swionibacillus sediminis gen. nov., sp. nov., a member of the family Bacillaceae isolated from ocean sediment.
[So] Source:Int J Syst Evol Microbiol;67(9):3440-3445, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, non-motile, strictly aerobic bacterium designated BW11-2T was isolated from marine sediment of the south-west Indian Ocean. Cells of BW11-2T were rod-shaped, endospore-forming, 0.3-0.5 µm wide, 1.8-2.0 µm long, catalase-positive and oxidase-negative. The isolate was capable of growing at 15-45 °C (optimum 30 °C), pH 5-9 (optimum 7) and with 0.5-10 % (w/v) NaCl (optimum 3 %). Based on 16S rRNA gene sequence similarities, BW11-2T was shown to belong to the family Bacillaceae within the phylum Firmicutes and formed a distinct lineage, showing the highest sequence similarities to closely related genera: Bacillus(93.9-94.7 %), Gracilibacillus (93.3-93.7 %), Amphibacillus (93.5 %), Virgibacillus (92.9-93.1 %) and Anaerobacillus(92.6-93.0 %). BW11-2T shared the highest 16S rRNA gene sequence similarity with the species Bacillus oleronius (94.7 %). The predominant fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0. The major quinone was menaquinone-7 (MK-7). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified aminolipid. The genomic DNA G+C content of strain BW11-2T was 43.3 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain BW11-2T represents a novel genus and species in the family Bacillaceae, for which the name Swionibacillus sediminis gen. nov., sp. nov. is proposed. Strain BW11-2T (=CICC 24196T=JCM 31924T) is the type strain.
[Mh] Termos MeSH primário: Bacillaceae/classificação
Sedimentos Geológicos/microbiologia
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Bacillaceae/genética
Bacillaceae/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Oceano Índico
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002133


  8 / 916 MEDLINE  
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[PMID]:28829034
[Au] Autor:Verma A; Ojha AK; Dastager SG; Natarajan R; Mayilraj S; Krishnamurthi S
[Ad] Endereço:1​Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
[Ti] Título:Domibacillus mangrovi sp. nov. and Domibacillus epiphyticus sp. nov., isolated from marine habitats of the central west coast of India.
[So] Source:Int J Syst Evol Microbiol;67(8):3063-3070, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:While studying culturable bacterial diversity in different marine habitats of the central west coast of India, two novel Gram-stain-positive, strictly aerobic, motile, endospore-forming and rod-shaped bacterial strains designated as SAOS 44T and SAB 38T were isolated from mangrove sediment soil and the surface of a macroalga, respectively. The strains were taxonomically characterized by using a polyphasic approach and genomic methods. The phylogenetic analysis based on 16S rRNA gene sequencing placed the strains firmly in the genus Domibacillus and were most closely related to Domibacillus antri KCTC 33636T. The predominant fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol in addition to an aminophosphoglycolipid. MK-6 was the only respiratory quinone. The range of values of digital DNA-DNA hybridization (19.2-24.9 %) and the ortho-average nucleotide identity (74.1-81.4 %) among strains SAOS 44T, SAB 38T and other Domibacillus species clearly supports their status as a distinct and novel species for which the names Domibacillusmangrovi sp. nov. SAOS 44T (=DSM 100930T=KCTC 33820T=MTCC 12571T) and Domibacillus epiphyticus sp. nov. SAB 38T (=DSM 100929T=KCTC 33830T=MTCC 12575T) are proposed, respectively.
[Mh] Termos MeSH primário: Bacillaceae/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Bacillaceae/genética
Bacillaceae/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Índia
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 71ANL51TLA (menaquinone 6)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002085


  9 / 916 MEDLINE  
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[PMID]:28732529
[Au] Autor:Chen X; Wang L; Zhou J; Wu H; Li D; Cui Y; Lu B
[Ad] Endereço:Department of Laboratory Medicine, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, China.
[Ti] Título:Exiguobacterium sp. A1b/GX59 isolated from a patient with community-acquired pneumonia and bacteremia: genomic characterization and literature review.
[So] Source:BMC Infect Dis;17(1):508, 2017 Jul 21.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bacterial species belonging to the genus Exiguobacterium are facultative anaerobic, non-spore-forming, Gram-positive bacilli, and rarely associated with human infections. Herein, we reported the first case of community-acquired pneumonia (CAP) and bacteremia due to Exiguobacterium spp. in China. CASE PRESENTATION: An adult male with severe CAP was hospitalized. The pathogen was isolated from his bloodstream and broncho-alveolar lavage fluid. The correct identification of the micro-organism was achieved using 16S rRNA sequencing, and its antibiotic susceptibility test was performed by microdilution method. The Whole Genome Sequencing (WGS) was used to characterize its genetic features and to elucidate its potential pathogenic mechanisms. Furthermore, its genome sequence was also compared with those of 3 publicly-available Exiguobacterium strains. A PubMed search was performed for further understanding the features of Exiguobacterium infections. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain GX59 was most closely related to Exiguobacterium AT1b (99.7%). The genome of GX59 was 2,727,929 bp in size, harbouring 2855 putative protein-coding genes, 5 rRNA operons, 37 tRNA genes and 1 tmRNA. The multiple genome comparison of 4 Exiguobacterium strains demonstrated that Exiguobacterium contained 37 genes of secretion systems, including sec, tat, FEA, Type IV Pili and competence-related DNA transformation transporter (Com). Virulence factors of the micro-organism included tlyC, NprR, MCP, Dam, which might play a critical role in causing lethal infection. CONCLUSIONS: The study highlighted the potential pathogenicity of the genus Exiguobacterium for its unique genes encoding various virulence factors and those associated with antibiotic resistance, therefore, its clinical significance should be valued.
[Mh] Termos MeSH primário: Bacillaceae/genética
Bacteriemia/microbiologia
Infecções Comunitárias Adquiridas/microbiologia
Filogenia
Pneumonia Bacteriana/microbiologia
[Mh] Termos MeSH secundário: Bacillaceae/isolamento & purificação
Bacillaceae/patogenicidade
China
DNA Bacteriano/genética
Diabetes Mellitus Tipo 2/microbiologia
Genoma Bacteriano
Seres Humanos
Masculino
Meia-Idade
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170723
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2616-1


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[PMID]:28699866
[Au] Autor:Rahi P; Kurli R; Khairnar M; Jagtap S; Pansare AN; Dastager SG; Shouche YS
[Ad] Endereço:1​Microbial Culture Collection, National Centre for Cell Science, Pune, Maharashtra 411007, India.
[Ti] Título:Description of Lysinibacillus telephonicus sp. nov., isolated from the screen of a cellular phone.
[So] Source:Int J Syst Evol Microbiol;67(7):2289-2295, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel bacterial strain, designated S5H2222T, was isolated form the screen of a cellular phone. The cells were Gram-stain-positive, rod-shaped, aerobic and motile, and endospores are formed. S5H2222T grew as pale white colonies on trypticase soy agar and the best growth was observed at 37 °C (10-55 °C) and at pH 7.0 (5.0-9.0). S5H2222T could tolerate up to 10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences placed this strain within the genus Lysinibacillus and it exhibited high 16S rRNA gene sequence similarity to Lysinibacillus halotolerans LAM612T (97.8 %), Lysinibacillus chungkukjangi 2RL3-2T (97.4 %) and Lysinibacillus sinduriensis BLB-1T (97.2 %). The DNA-DNA relatedness of the strain with L. halotolerans JCM 19611T, L. chungkukjangi KACC 16626T and L. sinduriensis KACC 16611T was 57, 64 and 55 % respectively. The genomic DNA G+C content was 39.8 mol%. The major fatty acids of S5H2222T were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. MK-7 was the only menaquinone and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, four unidentified polar lipids were also present. The diagnostic amino acids in the cell wall peptidoglycan contained Lys-Asp (type A4α). On the basis of the results of the phenotypic and genotypic characterizations, it was concluded that S5H2222T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillus telephonicus sp. nov. is proposed. The type strain is S5H2222T (=MCC 3065T=KACC 18714T=LMG 29294T).
[Mh] Termos MeSH primário: Bacillaceae/classificação
Telefone Celular
Filogenia
[Mh] Termos MeSH secundário: Bacillaceae/genética
Bacillaceae/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
Parede Celular/química
DNA Bacteriano/genética
Ácidos Graxos/química
Índia
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001943



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