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  1 / 4037 MEDLINE  
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[PMID]:29484747
[Au] Autor:Hirad AH; Ahmad J; Alkhedhairy AA; Bahkali AH; Khan ST
[Ad] Endereço:Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
[Ti] Título:Bacterial isolates exhibiting multidrug resistance, hemolytic activity, and high 16S rRNA gene similarity with well-known pathogens found in camel milk samples of Riyadh region.
[So] Source:APMIS;126(3):215-226, 2018 Mar.
[Is] ISSN:1600-0463
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Customary consumption of unpasteurized milk by the population in the central Najed region of Saudi Arabia may pose a health risk. Therefore, 80 camel milk samples were collected aseptically from seven different stations of Riyadh region. The biochemical and microbiological properties of these milk samples were determined. Nutrient agar and brain heart infusion agar were used to determine mesophilic aerobic counts (MACs). The MAC in each mL of milk varied from 60 to 16 × 10  CFU/mL on nutrient agar. Based on the colony morphology, 176 colonies were collected from different samples, and these isolates were de-replicated into 80 unique isolates using rep-PCR analysis. Surprisingly, the 16S rRNA sequence analysis of these strains revealed that more than one-third of the collected milk samples contained strains that share maximum sequence similarities with well-known pathogens, such as Brucella, Bacillus anthracis, Listeria monocytogenes, and MRSA. Furthermore, many strains exhibit 16S rRNA gene similarity with opportunistic pathogens such as Citrobacter freundii and Kytococcus schroeteri. Many strains exhibit ß-hemolytic activity and resistant to six different antibiotics. Our study suggested that consumption of raw camel milk from this region constitutes a great health risk.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Leite/química
Leite/microbiologia
[Mh] Termos MeSH secundário: Animais
Bacillus anthracis/genética
Bacillus anthracis/isolamento & purificação
Bactérias/genética
Carga Bacteriana
Brucella/genética
Brucella/isolamento & purificação
Camelus
Citrobacter freundii/genética
Citrobacter freundii/isolamento & purificação
Microbiologia de Alimentos
Seres Humanos
Listeria monocytogenes/genética
Listeria monocytogenes/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Micrococcaceae/genética
Micrococcaceae/isolamento & purificação
Pasteurização
RNA Ribossômico 16S/genética
Arábia Saudita
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180228
[St] Status:MEDLINE
[do] DOI:10.1111/apm.12802


  2 / 4037 MEDLINE  
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[PMID]:29377918
[Au] Autor:Steenkamp PJ; van Heerden H; van Schalkwyk OL
[Ad] Endereço:University of Pretoria, Faculty of Veterinary Science, Department of Production Animal Studies, Onderstepoort, South Africa.
[Ti] Título:Ecological suitability modeling for anthrax in the Kruger National Park, South Africa.
[So] Source:PLoS One;13(1):e0191704, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The spores of the soil-borne bacterium, Bacillus anthracis, which causes anthrax are highly resistant to adverse environmental conditions. Under ideal conditions, anthrax spores can survive for many years in the soil. Anthrax is known to be endemic in the northern part of Kruger National Park (KNP) in South Africa (SA), with occasional epidemics spreading southward. The aim of this study was to identify and map areas that are ecologically suitable for the harboring of B. anthracis spores within the KNP. Anthrax surveillance data and selected environmental variables were used as inputs to the maximum entropy (Maxent) species distribution modeling method. Anthrax positive carcasses from 1988-2011 in KNP (n = 597) and a total of 40 environmental variables were used to predict and evaluate their relative contribution to suitability for anthrax occurrence in KNP. The environmental variables that contributed the most to the occurrence of anthrax were soil type, normalized difference vegetation index (NDVI) and precipitation. Apart from the endemic Pafuri region, several other areas within KNP were classified as ecologically suitable. The outputs of this study could guide future surveillance efforts to focus on predicted suitable areas for anthrax, since the KNP currently uses passive surveillance to detect anthrax outbreaks.
[Mh] Termos MeSH primário: Antraz/diagnóstico
Bacillus anthracis/isolamento & purificação
Ecossistema
[Mh] Termos MeSH secundário: Antraz/epidemiologia
Surtos de Doenças
Seres Humanos
África do Sul/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191704


  3 / 4037 MEDLINE  
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[PMID]:29199987
[Au] Autor:Shornikov A; Tran H; Macias J; Halavaty AS; Minasov G; Anderson WF; Kuhn ML
[Ad] Endereço:Department of Chemistry and Biochemistry, San Francisco State University, USA.
[Ti] Título:Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-dehydrorhamnose 3,5-epimerase (RfbC).
[So] Source:Acta Crystallogr F Struct Biol Commun;73(Pt 12):664-671, 2017 Dec 01.
[Is] ISSN:2053-230X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The exosporium layer of Bacillus anthracis spores is rich in L-rhamnose, a common bacterial cell-wall component, which often contributes to the virulence of pathogens by increasing their adherence and immune evasion. The biosynthetic pathway used to form the activated L-rhamnose donor dTDP-L-rhamnose consists of four enzymes (RfbA, RfbB, RfbC and RfbD) and is an attractive drug target because there are no homologs in mammals. It was found that co-purifying and screening RfbC (dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase) from B. anthracis in the presence of the other three B. anthracis enzymes of the biosynthetic pathway yielded crystals that were suitable for data collection. RfbC crystallized as a dimer and its structure was determined at 1.63 Šresolution. Two different ligands were bound in the protein structure: pyrophosphate in the active site of one monomer and dTDP in the other monomer. A structural comparison with RfbC homologs showed that the key active-site residues are conserved across kingdoms.
[Mh] Termos MeSH primário: Bacillus anthracis/enzimologia
Proteínas de Bactérias/química
Carboidratos Epimerases/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Carboidratos Epimerases/metabolismo
Domínio Catalítico
Cristalografia por Raios X
Difosfatos/química
Difosfatos/metabolismo
Modelos Moleculares
Conformação Proteica
Homologia Estrutural de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Diphosphates); 4E862E7GRQ (diphosphoric acid); EC 5.1.3.- (Carbohydrate Epimerases); EC 5.1.3.13 (dTDP-4-ketorhamnose 3,5-epimerase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1107/S2053230X17015849


  4 / 4037 MEDLINE  
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[PMID]:29199984
[Au] Autor:Law A; Stergioulis A; Halavaty AS; Minasov G; Anderson WF; Kuhn ML
[Ad] Endereço:Department of Chemistry and Biochemistry, San Francisco State University, USA.
[Ti] Título:Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-dehydrorhamnose reductase (RfbD).
[So] Source:Acta Crystallogr F Struct Biol Commun;73(Pt 12):644-650, 2017 Dec 01.
[Is] ISSN:2053-230X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacillus anthracis is the causative agent of the deadly disease Anthrax. Its use in bioterrorism and its ability to re-emerge have brought renewed interest in this organism. B. anthracis is a Gram-positive bacterium that adds L-rhamnose to its cell-wall polysaccharides using the activated donor dTDP-ß-L-rhamnose. The enzymes involved in the biosynthesis of the activated donor are absent in humans, which make them ideal targets for therapeutic development to combat pathogens. Here, the 2.65 Šresolution crystal structure of the fourth enzyme in the dTDP-ß-L-rhamnose-biosynthetic pathway from B. anthracis, dTDP-4-dehydro-ß-L-rhamnose reductase (RfbD), is presented in complex with NADP . This enzyme catalyzes the reduction of dTDP-4-dehydro-ß-L-rhamnose to dTDP-ß-L-rhamnose. Although the protein was co-crystallized in the presence of Mg , the protein lacks the conserved residues that coordinate Mg .
[Mh] Termos MeSH primário: Bacillus anthracis/enzimologia
Proteínas de Bactérias/química
Desidrogenases de Carboidrato/química
Desidrogenases de Carboidrato/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Desidrogenases de Carboidrato/genética
Cristalografia por Raios X
Magnésio/metabolismo
Modelos Moleculares
NADP/química
NADP/metabolismo
Açúcares de Nucleosídeo Difosfato/metabolismo
Conformação Proteica
Multimerização Proteica
Homologia Estrutural de Proteína
Especificidade por Substrato
Nucleotídeos de Timina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Nucleoside Diphosphate Sugars); 0 (Thymine Nucleotides); 2147-59-3 (thymidine diphosphate rhamnose); 53-59-8 (NADP); EC 1.1.- (Carbohydrate Dehydrogenases); EC 1.1.1.133 (dTDP-4-dehydrorhamnose reductase); I38ZP9992A (Magnesium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1107/S2053230X17015746


  5 / 4037 MEDLINE  
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[PMID]:29198864
[Au] Autor:Jiao GS; Kim S; Moayeri M; Thai A; Cregar-Hernandez L; McKasson L; O'Malley S; Leppla SH; Johnson AT
[Ad] Endereço:Hawaii Biotech, 650 Iwilei Road, Suite 204, Honolulu, HI 96817, USA.
[Ti] Título:Small molecule inhibitors of anthrax edema factor.
[So] Source:Bioorg Med Chem Lett;28(2):134-139, 2018 01 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Anthrax is a highly lethal disease caused by the Gram-(+) bacteria Bacillus anthracis. Edema toxin (ET) is a major contributor to the pathogenesis of disease in humans exposed to B. anthracis. ET is a bipartite toxin composed of two proteins secreted by the vegetative bacteria, edema factor (EF) and protective antigen (PA). Our work towards identifying a small molecule inhibitor of anthrax edema factor is the subject of this letter. First we demonstrate that the small molecule probe 5'-Fluorosulfonylbenzoyl 5'-adenosine (FSBA) reacts irreversibly with EF and blocks enzymatic activity. We then show that the adenosine portion of FSBA can be replaced to provide more drug-like molecules which are up to 1000-fold more potent against EF relative to FSBA, display low cross reactivity when tested against a panel of kinases, and are nanomolar inhibitors of EF in a cell-based assay of cAMP production.
[Mh] Termos MeSH primário: Antraz/tratamento farmacológico
Bacillus anthracis/efeitos dos fármacos
Toxinas Bacterianas/antagonistas & inibidores
Bibliotecas de Moléculas Pequenas/farmacologia
[Mh] Termos MeSH secundário: Animais
Antígenos de Bactérias/farmacologia
Toxinas Bacterianas/farmacologia
AMP Cíclico/antagonistas & inibidores
AMP Cíclico/biossíntese
Relação Dose-Resposta a Droga
Seres Humanos
Camundongos
Estrutura Molecular
Proteínas Quinases/metabolismo
Células RAW 264.7
Bibliotecas de Moléculas Pequenas/síntese química
Bibliotecas de Moléculas Pequenas/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Bacterial Toxins); 0 (Small Molecule Libraries); 0 (anthrax toxin); E0399OZS9N (Cyclic AMP); EC 2.7.- (Protein Kinases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


  6 / 4037 MEDLINE  
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[PMID]:29284252
[Au] Autor:Pérez-Tanoira R; Ramos JM; Prieto-Pérez L; Tesfamariam A; Balcha S; Tissiano G; Cabello A; Cuadros J; Rodríguez-Valero N; Barreiro P; Reyes F; Górgolas M
[Ad] Endereço:Gambo Rural General Hospital, Kore, West-Arsi, Gambo, Ethiopia. ramontanoira@hotmail.com.
[Ti] Título:Diagnosis of cutaneous anthrax in resource-poor settings in West Arsi Province, Ethiopia.
[So] Source:Ann Agric Environ Med;24(4):712-715, 2017 Dec 23.
[Is] ISSN:1898-2263
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Cutaneous anthrax is a zoonotic disease caused by the spore-forming bacterium Bacillus anthracis, which typically presents with ulcers after contact with animals or animal products, and is rarely seen in high-income countries but is common in those with low- and middle-incomes. Objective. The aim of this study is to show the main clinical characteristics of cutaneous anthrax in endemic areas. MATERIAL AND METHODS: The study describes the main clinical characteristics of cutaneous anthrax in eight patients (six female and two male, age range 1 - 56 years) admitted to the rural General Hospital of Gambo, West Arsi Province of Ethiopia from 2010-2013. RESULTS: In all cases, lesions began as an erythematous papule located on exposed sites (n=7 head; n=1 thigh) and subsequently became a necrotic black eschar surrounded by an edematous halo. Two patients presented with painful ipsilateral adenopathy near the black eschar. Four patients developed a malignant pustule on the suborbital region of the face. Patients responded positively to treatment, and the lesions resolved, leaving eschars. However, one patient suffered the loss of an eyeball, and another died 12 hours after starting treatment. CONCLUSIONS: Physicians working in rural areas of resource-poor settings should be trained in the clinical identification of cutaneous anthrax. Early antibiotic treatment is essential for decreasing morbidity and mortality.
[Mh] Termos MeSH primário: Antraz/diagnóstico
Bacillus anthracis/fisiologia
Dermatopatias Bacterianas/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Antraz/tratamento farmacológico
Antraz/epidemiologia
Antraz/microbiologia
Antibacterianos/administração & dosagem
Bacillus anthracis/efeitos dos fármacos
Bacillus anthracis/genética
Bacillus anthracis/isolamento & purificação
Criança
Pré-Escolar
Etiópia/epidemiologia
Feminino
Seres Humanos
Lactente
Masculino
Meia-Idade
População Rural
Dermatopatias Bacterianas/tratamento farmacológico
Dermatopatias Bacterianas/epidemiologia
Dermatopatias Bacterianas/microbiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE


  7 / 4037 MEDLINE  
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[PMID]:29059238
[Au] Autor:Seshadri S; Allan DSJ; Carlyle JR; Zenewicz LA
[Ad] Endereço:Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America.
[Ti] Título:Bacillus anthracis lethal toxin negatively modulates ILC3 function through perturbation of IL-23-mediated MAPK signaling.
[So] Source:PLoS Pathog;13(10):e1006690, 2017 Oct.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacillus anthracis, the causative agent of anthrax, secretes lethal toxin that down-regulates immune functions. Translocation of B. anthracis across mucosal epithelia is key for its dissemination and pathogenesis. Group 3 innate lymphocytes (ILC3s) are important in mucosal barrier maintenance due to their expression of the cytokine IL-22, a critical regulator of tissue responses and repair during homeostasis and inflammation. We found that B. anthracis lethal toxin perturbed ILC3 function in vitro and in vivo, revealing an unknown IL-23-mediated MAPK signaling pathway. Lethal toxin had no effects on the canonical STAT3-mediated IL-23 signaling pathway. Rather lethal toxin triggered the loss of several MAP2K kinases, which correlated with reduced activation of downstream ERK1/2 and p38, respectively. Inhibition studies showed the importance of MAPK signaling in IL-23-mediated production of IL-22. Our finding that MAPK signaling is required for optimal IL-22 production in ILC3s may lead to new approaches for targeting IL-22 biology.
[Mh] Termos MeSH primário: Antraz/imunologia
Antígenos de Bactérias/imunologia
Bacillus anthracis/imunologia
Toxinas Bacterianas/imunologia
Linfócitos/imunologia
Sistema de Sinalização das MAP Quinases/imunologia
[Mh] Termos MeSH secundário: Animais
Bacillus anthracis/patogenicidade
Seres Humanos
Interleucina-23/imunologia
Interleucinas/imunologia
Camundongos
Virulência/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Bacterial Toxins); 0 (Interleukin-23); 0 (Interleukins); 0 (anthrax toxin); 0 (interleukin-22)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171024
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006690


  8 / 4037 MEDLINE  
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[PMID]:29048594
[Au] Autor:Marinier E; Zaheer R; Berry C; Weedmark KA; Domaratzki M; Mabon P; Knox NC; Reimer AR; Graham MR; Chui L; Patterson-Fortin L; Zhang J; Pagotto F; Farber J; Mahony J; Seyer K; Bekal S; Tremblay C; Isaac-Renton J; Prystajecky N; Chen J; Slade P; Van Domselaar G
[Ad] Endereço:National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington St, Winnipeg, MB R3E 3R2, Canada.
[Ti] Título:Neptune: a bioinformatics tool for rapid discovery of genomic variation in bacterial populations.
[So] Source:Nucleic Acids Res;45(18):e159, 2017 Oct 13.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ready availability of vast amounts of genomic sequence data has created the need to rethink comparative genomics algorithms using 'big data' approaches. Neptune is an efficient system for rapidly locating differentially abundant genomic content in bacterial populations using an exact k-mer matching strategy, while accommodating k-mer mismatches. Neptune's loci discovery process identifies sequences that are sufficiently common to a group of target sequences and sufficiently absent from non-targets using probabilistic models. Neptune uses parallel computing to efficiently identify and extract these loci from draft genome assemblies without requiring multiple sequence alignments or other computationally expensive comparative sequence analyses. Tests on simulated and real datasets showed that Neptune rapidly identifies regions that are both sensitive and specific. We demonstrate that this system can identify trait-specific loci from different bacterial lineages. Neptune is broadly applicable for comparative bacterial analyses, yet will particularly benefit pathogenomic applications, owing to efficient and sensitive discovery of differentially abundant genomic loci. The software is available for download at: http://github.com/phac-nml/neptune.
[Mh] Termos MeSH primário: Bactérias/genética
Biologia Computacional/métodos
Análise Mutacional de DNA/métodos
Estudos de Associação Genética
Técnicas Microbiológicas/métodos
Análise de Sequência de DNA/métodos
Software
[Mh] Termos MeSH secundário: Bacillus anthracis/genética
Regulação Bacteriana da Expressão Gênica
Genoma Bacteriano
Transcriptoma
Vibrio cholerae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx702


  9 / 4037 MEDLINE  
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[PMID]:29028799
[Au] Autor:Kracalik IT; Kenu E; Ayamdooh EN; Allegye-Cudjoe E; Polkuu PN; Frimpong JA; Nyarko KM; Bower WA; Traxler R; Blackburn JK
[Ad] Endereço:Spatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, FL, United States of America.
[Ti] Título:Modeling the environmental suitability of anthrax in Ghana and estimating populations at risk: Implications for vaccination and control.
[So] Source:PLoS Negl Trop Dis;11(10):e0005885, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Anthrax is hyper-endemic in West Africa. Despite the effectiveness of livestock vaccines in controlling anthrax, underreporting, logistics, and limited resources makes implementing vaccination campaigns difficult. To better understand the geographic limits of anthrax, elucidate environmental factors related to its occurrence, and identify human and livestock populations at risk, we developed predictive models of the environmental suitability of anthrax in Ghana. We obtained data on the location and date of livestock anthrax from veterinary and outbreak response records in Ghana during 2005-2016, as well as livestock vaccination registers and population estimates of characteristically high-risk groups. To predict the environmental suitability of anthrax, we used an ensemble of random forest (RF) models built using a combination of climatic and environmental factors. From 2005 through the first six months of 2016, there were 67 anthrax outbreaks (851 cases) in livestock; outbreaks showed a seasonal peak during February through April and primarily involved cattle. There was a median of 19,709 vaccine doses [range: 0-175 thousand] administered annually. Results from the RF model suggest a marked ecological divide separating the broad areas of environmental suitability in northern Ghana from the southern part of the country. Increasing alkaline soil pH was associated with a higher probability of anthrax occurrence. We estimated 2.2 (95% CI: 2.0, 2.5) million livestock and 805 (95% CI: 519, 890) thousand low income rural livestock keepers were located in anthrax risk areas. Based on our estimates, the current anthrax vaccination efforts in Ghana cover a fraction of the livestock potentially at risk, thus control efforts should be focused on improving vaccine coverage among high risk groups.
[Mh] Termos MeSH primário: Vacinas contra Antraz
Antraz/epidemiologia
Antraz/veterinária
Surtos de Doenças/veterinária
Gado
[Mh] Termos MeSH secundário: Algoritmos
Animais
Antraz/microbiologia
Antraz/prevenção & controle
Bacillus anthracis/isolamento & purificação
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/prevenção & controle
Clima
Simulação por Computador
Meio Ambiente
Métodos Epidemiológicos
Gana/epidemiologia
Seres Humanos
Concentração de Íons de Hidrogênio
Gado/microbiologia
Fatores de Risco
Solo/química
Vacinação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthrax Vaccines); 0 (Soil)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005885


  10 / 4037 MEDLINE  
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[PMID]:28981257
[Au] Autor:Schnicker NJ; Razzaghi M; Guha Thakurta S; Chakravarthy S; Dey M
[Ad] Endereço:Department of Chemistry, The University of Iowa , Iowa City, Iowa 52242, United States.
[Ti] Título:Bacillus anthracis Prolyl 4-Hydroxylase Interacts with and Modifies Elongation Factor Tu.
[So] Source:Biochemistry;56(43):5771-5785, 2017 Oct 31.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Prolyl hydroxylation is a very common post-translational modification and plays many roles in eukaryotes such as collagen stabilization, hypoxia sensing, and controlling protein transcription and translation. There is a growing body of evidence that suggests that prokaryotes contain prolyl 4-hydroxylases (P4Hs) homologous to the hypoxia-inducible factor (HIF) prolyl hydroxylase domain (PHD) enzymes that act on elongation factor Tu (EFTu) and are likely involved in the regulation of bacterial translation. Recent biochemical and structural studies with a PHD from Pseudomonas putida (PPHD) determined that it forms a complex with EFTu and hydroxylates a prolyl residue of EFTu. Moreover, while animal, plant, and viral P4Hs act on peptidyl proline, most prokaryotic P4Hs have been known to target free l-proline; the exceptions include PPHD and a P4H from Bacillus anthracis (BaP4H) that modifies collagen-like proline-rich peptides. Here we use biophysical and mass spectrometric methods to demonstrate that BaP4H recognizes full-length BaEFTu and a BaEFTu 9-mer peptide for site-specific proline hydroxylation. Using size-exclusion chromatography coupled small-angle X-ray scattering (SEC-SAXS) and binding studies, we determined that BaP4H forms a 1:1 heterodimeric complex with BaEFTu. The SEC-SAXS studies reveal dissociation of BaP4H dimeric subunits upon interaction with BaEFTu. While BaP4H is unusual within bacteria in that it is structurally and functionally similar to the animal PHDs and collagen P4Hs, respectively, this work provides further evidence of its promiscuous substrate recognition. It is possible that the enzyme might have evolved to hydroxylate a universally conserved protein in prokaryotes, similar to the PHDs, and implies a functional role in B. anthracis.
[Mh] Termos MeSH primário: Bacillus anthracis/metabolismo
Proteínas de Bactérias/metabolismo
Fator Tu de Elongação de Peptídeos/metabolismo
Prolil Hidroxilases/metabolismo
[Mh] Termos MeSH secundário: Bacillus anthracis/química
Bacillus anthracis/genética
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Fator Tu de Elongação de Peptídeos/química
Fator Tu de Elongação de Peptídeos/genética
Prolil Hidroxilases/química
Prolil Hidroxilases/genética
Ligação Proteica
Domínios Proteicos
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 1.14.11.- (Prolyl Hydroxylases); EC 3.6.1.- (Peptide Elongation Factor Tu)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00601



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