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[PMID]:28159274
[Au] Autor:Toopcham T; Mes JJ; Wichers HJ; Yongsawatdigul J
[Ad] Endereço:School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
[Ti] Título:Immunomodulatory activity of protein hydrolysates derived from Virgibacillus halodenitrificans SK1-3-7 proteinase.
[So] Source:Food Chem;224:320-328, 2017 Jun 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Modulation of inflammation-related immune response on THP-1 macrophages of protein hydrolysates derived from tilapia mince, casein and pea protein, were investigated. The protein substrates were hydrolyzed by Virgibacillus halodenitrificans SK1-3-7 proteinase. The degree of hydrolysis (DH) of casein was observed to be the highest throughout the course of hydrolysis. When challenging THP-1 macrophages, tilapia mince hydrolysate (TMH) enhanced innate immunity through induction of IL-1ß and COX-2 expression. Anti-inflammatory activity was observed in casein hydrolysate (CH) and pea protein hydrolysate (PPH) by attenuating lipopolysaccharide- (LPS) induced pro-inflammatory gene expression in THP-1 macrophages. CH suppressed IL-1ß, IL-6, IL-8, TNF-α and COX-2, while PPH reduced LPS-induced IL-6 and TNF-α responses. In addition, CH and PPH showed stronger suppression of LPS-induced pro-inflammatory gene expression compared with non-hydrolyzed casein and pea protein. These results suggest that TMH, CH and PPH prepared from V. halodenitrificans SK1-3-7 proteinase are potential functional food ingredients with immunomodulatory activity.
[Mh] Termos MeSH primário: Caseínas/farmacologia
Proteínas de Peixes/farmacologia
Peptídeo Hidrolases/metabolismo
Hidrolisados de Proteína/farmacologia
Virgibacillus/enzimologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/farmacologia
Células Cultivadas
Ciclídeos
Ciclo-Oxigenase 2/genética
Ciclo-Oxigenase 2/metabolismo
Regulação da Expressão Gênica
Seres Humanos
Hidrólise
Imunidade Inata
Imunomodulação
Inflamação
Interleucina-1beta/genética
Interleucina-1beta/metabolismo
Interleucina-6/genética
Interleucina-6/metabolismo
Interleucina-8/genética
Interleucina-8/metabolismo
Lipopolissacarídeos/toxicidade
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
Ervilhas/química
Proteínas de Plantas/farmacologia
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Caseins); 0 (Fish Proteins); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Interleukin-8); 0 (Lipopolysaccharides); 0 (Plant Proteins); 0 (Protein Hydrolysates); 0 (Tumor Necrosis Factor-alpha); 65072-00-6 (casein hydrolysate); EC 1.14.99.1 (Cyclooxygenase 2); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE


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[PMID]:27957631
[Au] Autor:Amziane M; Darenfed-Bouanane A; Abderrahmani A; Selama O; Jouadi L; Cayol JL; Nateche F; Fardeau ML
[Ad] Endereço:Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biology, USTHB, BP 32, El Alia, Bab Ezzouar, Algiers, Algeria.
[Ti] Título:Virgibacillus ainsalahensis sp. nov., a Moderately Halophilic Bacterium Isolated from Sediment of a Saline Lake in South of Algeria.
[So] Source:Curr Microbiol;74(2):219-223, 2017 Feb.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A Gram-positive, moderately halophilic, endospore-forming bacterium, designated MerV , was isolated from a sediment sample of a saline lake located in Ain Salah, south of Algeria. The cells were rod shaped and motile. Isolate MerV grew at salinity interval of 0.5-25% NaCl (optimum, 5-10%), pH 6.0-12.0 (optimum, 8.0), and temperature between 10 and 40 °C (optimum, 30 °C).The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, a phospholipid, and two lipids, and MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C and anteiso C . The DNA G+C content was 45.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MerV was most closely related to Virgibacillus halodenitrificans (gene sequence similarity of 97.0%). On the basis of phenotypic, chemotaxonomic properties, and phylogenetic analyses, strain MerV (=DSM = 28944 ) should be placed in the genus Virgibacillus as a novel species, for which the name Virgibacillus ainsalahensis is proposed.
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Virgibacillus/classificação
Virgibacillus/isolamento & purificação
[Mh] Termos MeSH secundário: Argélia
Composição de Bases
Análise por Conglomerados
Citosol/química
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Ácidos Graxos/análise
Glicolipídeos/análise
Lagos
Locomoção
Fosfolipídeos/análise
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Cloreto de Sódio/metabolismo
Temperatura Ambiente
Virgibacillus/genética
Virgibacillus/fisiologia
Vitamina K 2/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Fatty Acids); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-016-1171-0


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[PMID]:27855889
[Au] Autor:Toopcham T; Mes JJ; Wichers HJ; Roytrakul S; Yongsawatdigul J
[Ad] Endereço:School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
[Ti] Título:Bioavailability of angiotensin I-converting enzyme (ACE) inhibitory peptides derived from Virgibacillus halodenitrificans SK1-3-7 proteinases hydrolyzed tilapia muscle proteins.
[So] Source:Food Chem;220:190-197, 2017 Apr 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The angiotensin I-converting enzyme (ACE) inhibitory activity of protein hydrolysates from tilapia muscle fractions, namely mince (M), washed mince (WM), and sarcoplasmic protein (SP), were investigated. Each fraction was hydrolyzed by Virgibacillus halodenitrificans SK1-3-7 proteinases for up to 24h. After 8h of hydrolysis, the M hydrolysate (48% degree of hydrolysis (DH)) showed the highest ACE inhibitory activity, with an IC value of 0.54mg/ml, while the SP hydrolysate exhibited the lowest DH and ACE inhibition. In vitro gastrointestinal digestion reduced the ACE inhibitory activity of the M hydrolysate but enhanced its transport across Caco-2 cell monolayers. The transported peptides were found to contain 3-4 amino acid residues showing strong ACE inhibition. The novel ACE inhibitory peptide with the highest inhibition was found to be MCS, with an IC value of 0.29µM. Therefore, tilapia mince hydrolyzed by V. halodenitrificans proteinases contained ACE inhibitory peptides that are potentially bioavailable.
[Mh] Termos MeSH primário: Inibidores da Enzima Conversora de Angiotensina/farmacologia
Proteínas Musculares/farmacologia
Peptídeo Hidrolases/metabolismo
Peptídeos/farmacologia
Hidrolisados de Proteína/farmacologia
Virgibacillus/enzimologia
[Mh] Termos MeSH secundário: Animais
Disponibilidade Biológica
Células CACO-2
Seres Humanos
Tilápia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiotensin-Converting Enzyme Inhibitors); 0 (Muscle Proteins); 0 (Peptides); 0 (Protein Hydrolysates); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


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[PMID]:27106915
[Au] Autor:Tao P; Li H; Yu Y; Gu J; Liu Y
[Ad] Endereço:State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, State Key Laboratory of Biological Reactor Engineering, School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republ
[Ti] Título:Ectoine and 5-hydroxyectoine accumulation in the halophile Virgibacillus halodenitrificans PDB-F2 in response to salt stress.
[So] Source:Appl Microbiol Biotechnol;100(15):6779-89, 2016 Aug.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The moderately halophilic bacterium Virgibacillus halodenitrificans PDB-F2 copes with salinity by synthesizing or taking up compatible solutes. The main compatible solutes in this strain were ectoine and hydroxyectoine, as determined by (1)H nuclear magnetic resonance spectroscopy ((1)H-NMR). A high-performance liquid chromatography (HPLC) analysis showed that ectoine was the major solute that was synthesized in response to elevated salinity, while hydroxyectoine was a minor solute. However, the hydroxyectoine/ectoine ratio increased from 0.04 at 3 % NaCl to 0.45 at 15 % NaCl in the late exponential growth phase. A cluster of ectoine biosynthesis genes was identified, including three genes in the order of ectA, ectB, and ectC. The hydroxyectoine biosynthesis gene ectD was not part of the ectABC gene cluster. Reverse transcription-quantitative polymerase chain reactions (RT-qPCR) showed that the expression of the ect genes was salinity dependent. The expression of ectABC reached a maximum at 12 % NaCl, while ectD expression increased up to 15 % NaCl. Ectoine and hydroxyectoine production was growth phase dependent. The hydroxyectoine/ectoine ratio increased from 0.018 in the early exponential phase to 0.11 in the stationary phase at 5 % NaCl. Hydroxyectoine biosynthesis started much later than ectoine biosynthesis after osmotic shock, and the temporal expression of the ect genes differed under these conditions, with the ectABC genes being expressed first, followed by ectD gene. Increased culture salinity triggered ectoine or hydroxyectoine uptake when they were added to the medium. Hydroxyectoine was accumulated preferentially when both ectoine and hydroxyectoine were provided exogenously.
[Mh] Termos MeSH primário: Diamino Aminoácidos/metabolismo
Pressão Osmótica/fisiologia
Cloreto de Sódio/farmacologia
Virgibacillus/metabolismo
[Mh] Termos MeSH secundário: Diamino Aminoácidos/genética
Regulação Bacteriana da Expressão Gênica
Salinidade
Tolerância a Sal/genética
Tolerância a Sal/fisiologia
Estresse Fisiológico/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Diamino); 0 (hydroxyectoine); 451W47IQ8X (Sodium Chloride); 7GXZ3858RY (ectoine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170202
[Lr] Data última revisão:
170202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160424
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-016-7549-x


  5 / 30 MEDLINE  
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[PMID]:27052702
[Au] Autor:Udomsil N; Chen S; Rodtong S; Yongsawatdigul J
[Ad] Endereço:School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakon Ratchasima, 30000, Thailand; Microbial Cultures Research Center for Food and Bioplastics Production, Suranaree University of Technology, Thailand. Electronic address: paeng888@hotmail.com.
[Ti] Título:Quantification of viable bacterial starter cultures of Virgibacillus sp. and Tetragenococcus halophilus in fish sauce fermentation by real-time quantitative PCR.
[So] Source:Food Microbiol;57:54-62, 2016 Aug.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Real-time quantitative polymerase chain reaction (qPCR) methods were developed for the quantification of Virgibacillus sp. SK37 and Tetragenococcus halophilus MS33, which were added as starter cultures in fish sauce fermentation. The PCR assays were coupled with propidium monoazide (PMA) treatment of samples to selectively quantify viable cells and integrated with exogenous recombinant Escherichia coli cells to control variabilities in analysis procedures. The qPCR methods showed species-specificity for both Virgibacillus halodenitrificans and T. halophilus as evaluated using 6 reference strains and 28 strains of bacteria isolated from fish sauce fermentation. The qPCR efficiencies were 101.1% for V. halodenitrificans and 90.2% for T. halophilus. The quantification limits of the assays were 10(3) CFU/mL and 10(2) CFU/mL in fish sauce samples with linear correlations over 4 Logs for V. halodenitrificans and T. halophilus, respectively. The matrix effect was not observed when evaluated using fish sauce samples fermented for 1-6 months. The developed PMA-qPCR methods were successfully applied to monitor changes of Virgibacillus sp. SK37 and T. halophilus MS33 in a mackerel fish sauce fermentation model where culture-dependent techniques failed to quantify the starter cultures. The results demonstrated the usability of the methods as practical tools for monitoring the starter cultures in fish sauce fermentation.
[Mh] Termos MeSH primário: Enterococcaceae/metabolismo
Produtos Pesqueiros/microbiologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Virgibacillus/metabolismo
[Mh] Termos MeSH secundário: Animais
Enterococcaceae/genética
Fermentação
Peixes
Virgibacillus/genética
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160408
[St] Status:MEDLINE


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[PMID]:26873558
[Au] Autor:Kobayashi T; Agustini TW; Ibrahim R; Kamei K; Kondo A; Kajiwara M; Ooka Y; Nakamura H; Terahara T; Imada C
[Ad] Endereço:Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan. takeshik@kaiyodai.ac.jp.
[Ti] Título:Production of bacteriocin by Virgibacillus salexigens isolated from "terasi": a traditionally fermented shrimp paste in Indonesia.
[So] Source:World J Microbiol Biotechnol;32(3):47, 2016 Mar.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A natural antibacterial-substance-producing gram-positive bacterium was isolated from terasi shrimp paste, a popular fermented product in Indonesia. This strain, a spore-forming and strictly aerobic bacterium, was identified as Virgibacillus salexigens by 16S rRNA gene sequence analysis. The antibacterial substance purified from the precipitated product in the culture supernatant of the strain using ammonium sulfate showed a broad inhibition spectrum against gram-positive bacteria, including a typical foodborne bacterium, namely, Listeria monocytogenes. The antibacterial activity of the substance was inactivated by treatments with various proteolytic enzymes. It was stable after heating or pH treatment, and approximately 60% of the initial activity remained even after heating at 121 °C for 15 min. In addition, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis indicated that its monoisotopic mass weight was 5318.4 Da (M+H)(+). On the basis of the results obtained by the automated Edman degradation technique and MALDI-TOF MS analysis, the substance can be classified as a member of Class IId bacteriocins, but it could not be identified as any of the previously purified substances except for the putative bacteriocin predicted from the draft genome sequence data of gram-positive bacteria such as Virgibacillus and Bacillus strains.
[Mh] Termos MeSH primário: Bacteriocinas/metabolismo
Bacteriocinas/farmacologia
Microbiologia de Alimentos
Bactérias Gram-Positivas/efeitos dos fármacos
Virgibacillus/isolamento & purificação
Virgibacillus/metabolismo
[Mh] Termos MeSH secundário: Aerobiose
Bacteriocinas/isolamento & purificação
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Concentração de Íons de Hidrogênio
Indonésia
Peso Molecular
Proteólise
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Esporos Bacterianos/citologia
Temperatura Ambiente
Virgibacillus/classificação
Virgibacillus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacteriocins); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160214
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-015-1991-2


  7 / 30 MEDLINE  
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[PMID]:26869011
[Au] Autor:Daroonpunt R; Tanasupawat S; Kudo T; Ohkuma M; Itoh T
[Ad] Endereço:1​Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
[Ti] Título:Virgibacillus kapii sp. nov., isolated from Thai shrimp paste (Ka-pi).
[So] Source:Int J Syst Evol Microbiol;66(4):1832-7, 2016 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-reaction-positive and rod-shaped bacterium, designated KN3-8-4T, was isolated from shrimp paste collected from a market in Nakhon Sri Thammarat province, Thailand. Strain KN3-8-4T was a strictly aerobic, motile bacterium that produced ellipsoidal endospores at a terminal position. The isolate grew at pH 4.5-10 (optimum pH 7.5), at 11-42 °C (optimum 37 °C) and in the presence of 0-15% (w/v) NaCl (optimum 1-5%). On the basis of 16S rRNA gene sequences, strain KN3-8-4T belonged to the genus Virgibacillus and showed highest similarity with Virgibacillus olivae JCM 30551T (97.85% 16S rRNA gene sequence similarity). The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid and the major isoprenoid quinone was MK-7. Strain KN3-8-4T contained anteiso-C15:0 and anteiso-C17:0 as major cellular fatty acids and had phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and one glycolipid as polar lipids. The DNA G+C content was 43.5 mol%. Strain KN3-8-4T showed low DNA-DNA relatedness (20.44%) with V. olivae JCM 30551T. On the basis of phenotypic and chemotaxonomic data and phylogenetic analyses, strain KN3-8-4T represents a novel species of the genus Virgibacillus for which the name Virgibacillus kapii sp. nov. is proposed. The type strain is KN3-8-4T (=JCM 30071T=LMG 28282T=TISTR 2279T=PCU 345T).
[Mh] Termos MeSH primário: Microbiologia de Alimentos
Filogenia
Alimentos Marinhos/microbiologia
Virgibacillus/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Glicolipídeos/química
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Tailândia
Virgibacillus/genética
Virgibacillus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160422
[Lr] Data última revisão:
160422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.000951


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[PMID]:26520833
[Au] Autor:Hamza A; Fdhila K; Zouiten D; Masmoudi AS
[Ad] Endereço:Institut National des Sciences et Technologies de la Mer, 5000, Monastir, Tunisie. ahlem.hamza@yahoo.fr.
[Ti] Título:Virgibacillus proomii and Bacillus mojavensis as probiotics in sea bass (Dicentrarchus labrax) larvae: effects on growth performance and digestive enzyme activities.
[So] Source:Fish Physiol Biochem;42(2):495-507, 2016 Apr.
[Is] ISSN:1573-5168
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study examined the effects of two probiotics (Virgibacillus proomii and Bacillus mojavensis) on the digestive enzyme activity, survival and growth of Dicentrarchus labrax at various ontogenetic stages in three separate experiments. These probiotics were incorporated as single or mixed into fish feed for a period of 60 days. The growth parameters, proximate composition of whole body, digestive enzymes and gut microbiology were monitored at regular. The increments in length and weight and the survival were significantly higher (P < 0.05), and the values of food conversions were significantly lower (P < 0.05) in fishes fed the probiotic. The administration of V. proomii and B. mojavensis in diet resulted in an increase (P > 0.05) in body ash and protein content and in the specific activity of phosphatase alkaline and amylase in the digestive tract of all the fishes. V. proomii and B. mojavensis persisted in the fish intestine and in the feed in high numbers during the feeding period (group 1: 5.8 × 10(4) CFU/ml, group 2: 9.6 × 10(4) CFU/ml, and group 3: 9.8 × 10(4) CFU/ml day 60). The two probiotics V. proomii and B. mojavensis were adequate for improved growth performance and survival and for healthy gut microenvironment of the host.
[Mh] Termos MeSH primário: Ração Animal
Aquicultura
Bacillus
Bass/fisiologia
Probióticos
Virgibacillus
[Mh] Termos MeSH secundário: Animais
Dieta/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151102
[St] Status:MEDLINE
[do] DOI:10.1007/s10695-015-0154-6


  9 / 30 MEDLINE  
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[PMID]:25820449
[Au] Autor:Montriwong A; Rodtong S; Yongsawatdigul J
[Ad] Endereço:School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
[Ti] Título:Detergent-Stable Salt-Activated Proteinases from Virgibacillus halodenitrificans SK1-3-7 Isolated from Fish Sauce Fermentation.
[So] Source:Appl Biochem Biotechnol;176(2):505-17, 2015 May.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The NaCl-activated and detergent-stable proteinases from Virgibacillus halodenitrificans SK1-3-7 isolated from fish sauce fermentation were purified and characterized. The enzymes with molecular masses of 20 and 36 kDa showed caseinolytic activity on a zymogram. Optimum azocaseinolytic activity was at 60 °C and pH 9. The proteolytic activity increased in the presence of 10 mM CaCl2 and 0.5 M NaCl and showed high stability at 0-2 M NaCl. The enzymes were stable at pH 4-10 and 10-50 °C. The enzymes preferably hydrolyzed Suc-Ala-Ala-Pro-Phe-pNA and were completely inhibited by phenylmethanesulfonyl fluoride (PMSF), showing subtilisin-like characteristics. Activity and stability remained high in the presence of H2O2 and various surfactants. The enzymes exhibited high stability (>95%) in various organic solvents (DMSO, butanol, ethanol, 2-propanol, and acetonitrile) at concentration of 50%. The V. halodenitrificans SK1-3-7 proteinases showed potential as a biocatalyst in aqueous-organic solvent systems and as an additive in laundry detergent.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Detergentes/química
Produtos Pesqueiros/microbiologia
Peptídeo Hidrolases/metabolismo
Cloreto de Sódio/metabolismo
Virgibacillus/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Estabilidade Enzimática
Temperatura Alta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Detergents); 451W47IQ8X (Sodium Chloride); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150521
[Lr] Data última revisão:
150521
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150331
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-015-1591-5


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[PMID]:25301543
[Au] Autor:Yin X; Yang Y; Wang S; Zhang G
[Ad] Endereço:Laboratory of Ocean and Coast Geology, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
[Ti] Título:Virgibacillus oceani sp. nov. isolated from ocean sediment.
[So] Source:Int J Syst Evol Microbiol;65(Pt 1):159-64, 2015 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-positive, moderately halophilic, motile, strictly aerobic, endospore-forming, rod-shaped bacterium, strain MY11(T), was isolated from a sediment sample collected from the Western Pacific. This isolate grew in the presence of 0.5-18% (w/v) NaCl and at pH 6.0-10.0 and 15-45 °C; optimum growth was observed with 3.5% (w/v) NaCl and at pH 8.0-9.0 and 35-37 °C. Strain MY11(T) had menaquinone 7 (MK-7) as the predominant respiratory quinone and anteiso-C15:0 and anteiso-C17:0 as major fatty acids. Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 34.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain MY11(T) was a member of the genus Virgibacillus, exhibiting sequence similarities of 95.3-97.6% to the type strains of recognized Virgibacillus species. Strain MY11(T) could be differentiated from recognized species of the genus Virgibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. On the basis of the data presented, strain MY11(T) is considered to represent a novel species of the genus Virgibacillus, for which the name Virgibacillus oceani sp. nov. is proposed. The type strain is MY11(T) ( =LMG 28105(T) =CGMCC 1.12754(T) =MCCC 1A09973(T)).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Filogenia
Água do Mar/microbiologia
Virgibacillus/classificação
[Mh] Termos MeSH secundário: Composição de Bases
DNA Bacteriano/genética
DNA Ribossômico/genética
Ácidos Graxos/química
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
Oceano Pacífico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Virgibacillus/genética
Virgibacillus/isolamento & purificação
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:150120
[Lr] Data última revisão:
150120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141011
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.068213-0



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