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[PMID]:29458566
[Au] Autor:Kim YS; Cha CJ
[Ad] Endereço:Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.
[Ti] Título:Paenibacillus translucens sp. nov., isolated from tidal flat sediment.
[So] Source:Int J Syst Evol Microbiol;68(3):936-941, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11 , was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea. Strain CJ11 grew optimally on R2A at 30 °C and pH 7.0. Sequencing results of the 16S rRNA gene revealed that strain CJ11 possesses two copies of the 16S rRNA gene varying at five nucleotide positions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11 belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691 (99.36-99.15 % similarity). DNA-DNA relatedness levels of strain CJ11 was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691 . The G+C content of the genomic DNA was 51.0 mol%. Strain CJ11 contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major isoprenoid quinone was menaquinone-7. The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids. On the basis of the polyphasic taxonomic study, strain CJ11 represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp. nov. is proposed. The type strain is CJ11 (=KACC 19304 =JCM 32080 ).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Paenibacillus/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Glicolipídeos/química
Paenibacillus/genética
Paenibacillus/isolamento & purificação
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 583-93-7 (Diaminopimelic Acid); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002613


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[PMID]:29351296
[Au] Autor:Passera A; Marcolungo L; Casati P; Brasca M; Quaglino F; Cantaloni C; Delledonne M
[Ad] Endereço:Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, Università degli Studi di Milano, Milan, Italy.
[Ti] Título:Hybrid genome assembly and annotation of Paenibacillus pasadenensis strain R16 reveals insights on endophytic life style and antifungal activity.
[So] Source:PLoS One;13(1):e0189993, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria of the Paenibacillus genus are becoming important in many fields of science, including agriculture, for their positive effects on the health of plants. However, there are little information available on this genus compared to other bacteria (such as Bacillus or Pseudomonas), especially when considering genomic information. Sequencing the genomes of plant-beneficial bacteria is a crucial step to identify the genetic elements underlying the adaptation to life inside a plant host and, in particular, which of these features determine the differences between a helpful microorganism and a pathogenic one. In this study, we have characterized the genome of Paenibacillus pasadenensis, strain R16, recently investigated for its antifungal activities and plant-associated features. An hybrid assembly approach was used integrating the very precise reads obtained by Illumina technology and long fragments acquired with Oxford Nanopore Technology (ONT) sequencing. De novo genome assembly based solely on Illumina reads generated a relatively fragmented assembly of 5.72 Mbp in 99 ungapped sequences with an N50 length of 544 Kbp; hybrid assembly, integrating Illumina and ONT reads, improved the assembly quality, generating a genome of 5.75 Mbp, organized in 6 contigs with an N50 length of 3.4 Mbp. Annotation of the latter genome identified 4987 coding sequences, of which 1610 are hypothetical proteins. Enrichment analysis identified pathways of particular interest for the endophyte biology, including the chitin-utilization pathway and the incomplete siderophore pathway which hints at siderophore parasitism. In addition the analysis led to the identification of genes for the production of terpenes, as for example farnesol, that was hypothesized as the main antifungal molecule produced by the strain. The functional analysis on the genome confirmed several plant-associated, plant-growth promotion, and biocontrol traits of strain R16, thus adding insights in the genetic bases of these complex features, and of the Paenibacillus genus in general.
[Mh] Termos MeSH primário: Endófitos/genética
Fungos/patogenicidade
Genoma Bacteriano
Paenibacillus/genética
Plantas/microbiologia
[Mh] Termos MeSH secundário: Amino Açúcares/metabolismo
Metabolismo dos Carboidratos
DNA Bacteriano/genética
DNA Bacteriano/isolamento & purificação
Endófitos/metabolismo
Endófitos/fisiologia
Sequenciamento de Nucleotídeos em Larga Escala
Compostos Orgânicos/metabolismo
Paenibacillus/metabolismo
Paenibacillus/fisiologia
Desenvolvimento Vegetal
Sideróforos/biossíntese
Esporos Bacterianos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Sugars); 0 (DNA, Bacterial); 0 (Organic Chemicals); 0 (Siderophores)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189993


  3 / 703 MEDLINE  
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[PMID]:29235981
[Au] Autor:Nahar S; Cha CJ
[Ad] Endereço:Department of Systems Biotechnology, Chung-Ang University, Anseong 456-756, Republic of Korea.
[Ti] Título:Paenibacillus limicola sp. nov., isolated from tidal flat sediment.
[So] Source:Int J Syst Evol Microbiol;68(1):423-426, 2018 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An aerobic, Gram-staining-variable, rod-shaped, endospore-forming and motile bacterial strain, designated CJ6 , was isolated from a tidal flat on Ganghwa Island, South Korea. The isolate was characterized based on a polyphasic taxonomy approach. Strain CJ6 grew optimally on R2A agar media at 30 °C and pH 7. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CJ6 belonged to the genus Paenibacillus, displaying the highest sequence similarity to Paenibacillus vulneris CCUG 53270 (97.0 %) and clearly defined strain CJ6 as a novel species within the genus. The G+C content of the genomic DNA was 49.9 mol%. The major polar lipid contents of strain CJ6 were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified glycolipids. MK-7 was detected as the major respiratory quinone. The dominant fatty acid was anteiso-C15 : 0. Analyses of phylogenetic, phenotypic, biochemical and chemotaxonomic characteristics indicated that strain CJ6 was distinguishable from its closely related type strains. Therefore, strain CJ6 represents a novel species in the genus Paenibacillus, for which name Paenibacilluslimicola sp. nov. is proposed; the type strain is CJ6 (=KACC 19303 =JCM 32079 ).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Paenibacillus/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Paenibacillus/genética
Paenibacillus/isolamento & purificação
Fosfatidiletanolaminas/química
Fosfatidilgliceróis/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylethanolamines); 0 (Phosphatidylglycerols); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 39382-08-6 (phosphatidylethanolamine); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002522


  4 / 703 MEDLINE  
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[PMID]:28923247
[Au] Autor:Chen YH; Chiu CC; Hung SW; Liu JY; Wang YC; Lv Q; Hsu CC; Huang YW; Huang WC; Chuang HL; Chen TH
[Ad] Endereço:Graduate Institute of Veterinary Pathobiology, National Chung Hsin University, Taichung 402, Taiwan.
[Ti] Título:Effects of plant- and animal-based high-fat diets on lipid storage and distribution in environmental bacteria-colonized gnotobiotic mice.
[So] Source:Biochem Biophys Res Commun;493(2):1075-1081, 2017 Nov 18.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Different edible oils such as lard and soybean oil have been reported to interact with the gut microbiota, affecting host lipid metabolism. However, whether bacteria derived from the environment influence host lipid metabolism remains unclear. This study aimed to clarify the roles of environmental bacteria in host lipid storage and distribution with various edible oils. Gnotobiotic C57BL/6JNarl mice were inoculated with Lysinibacillus xylanilyticus and Paenibacillus azoreducens and then fed either a normal diet (LabDiet 5010, control group) or a diet containing 60% lard (L-group) or soybean oil (S-group) for 18 months. Interestingly, the S-group accumulated massive amounts of white adipose tissue compared to the L- and control groups, while the L-group displayed more hepatic steatosis and fatty droplets than the other groups. The expression of fatty acid synthase (FAS), hydroxymethylglutaryl-coenzyme A reductase (HMGCR), sterol regulatory element-binding protein 2 (SREBP2), and peroxisome proliferator-activated receptor gamma (PPARγ) in the livers of the L-group were markedly elevated compared to the S-group. FAS and PPARγ protein levels were also markedly elevated. However, there were no differences in the expression of the pro-inflammatory cytokines interleukin-6 and tumor necrosis factor-α between the groups. Our results suggest that environmental bacteria may affect host hepatic inflammation and lipid distribution in the presence of high-fat diets, with different effects depending on the fat type consumed.
[Mh] Termos MeSH primário: Dieta Hiperlipídica/efeitos adversos
Fígado Gorduroso/metabolismo
Fígado Gorduroso/microbiologia
Metabolismo dos Lipídeos
Fígado/metabolismo
Fígado/microbiologia
[Mh] Termos MeSH secundário: Animais
Bacillaceae/fisiologia
Gorduras na Dieta/efeitos adversos
Gorduras na Dieta/metabolismo
Fígado Gorduroso/patologia
Fígado/patologia
Masculino
Camundongos Endogâmicos C57BL
Paenibacillus/fisiologia
Óleo de Soja/efeitos adversos
Óleo de Soja/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fats); 8001-22-7 (Soybean Oil); SI6O3IW77Z (lard)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


  5 / 703 MEDLINE  
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[PMID]:28881947
[Au] Autor:Murphey Coy R; Held DW; Kloepper JW
[Ad] Endereço:Department of Entomology and Plant Pathology, Auburn University, 301 Funchess Hall, Auburn, AL 36849.
[Ti] Título:Bacterial Inoculant Treatment of Bermudagrass Alters Ovipositional Behavior, Larval and Pupal Weights of the Fall Armyworm (Lepidoptera: Noctuidae).
[So] Source:Environ Entomol;46(4):831-838, 2017 08 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nonpathogenic soil bacteria can colonize the rhizosphere and induce unique plant phenotypes that may influence plant-insect interactions. However, few studies have considered the influences of bacteria-plant interactions on insect feeding and oviposition. The objective of this study was to determine how rhizobacterial inoculation of bermudagrass affects larval development and ovipositional behaviors of the fall armyworm (Spodoptera frugiperda J.E. Smith). Eight blends of rhizobacteria known to induce root or shoot growth in grasses were applied weekly to hybrid bermudagrass for 5 wk. Oviposition was evaluated in two no-choice trials with bacteria-treated, fertilized, or nontreated grass. Grass blades from these treatments were extracted in polar and nonpolar solvents and assayed for oviposition responses. Another experiment compared the development of fall armyworm larvae on bermudagrass treated with each of the eight rhizobacterial blends for 5 wk to larvae fed nontreated bermudagrass. Females deposited more eggs on nontreated and fertilized grass and ≤34% of eggs on grass treated with rhizobacterial blends. Moths exposed to polar and nonpolar extracts were unable to reproduce these results. Larval and pupal weights at days 10 and 12 and the number of adults to eclose were lower for larvae fed some, but not all, bacteria-treated bermudagrass relative to controls. This is one of the few studies to investigate plant-microbe-insect interactions in an economically important system. Although the effects noted with fall armyworm are limited, induced changes in roots also reported for these bacteria may have greater utility than foliar changes for mediating interactions with biotic or abiotic stresses.
[Mh] Termos MeSH primário: Inoculantes Agrícolas/química
Bacillales/química
Cynodon/microbiologia
Mariposas/microbiologia
Mariposas/fisiologia
Controle Biológico de Vetores
[Mh] Termos MeSH secundário: Animais
Bacillus/química
Brevibacillus/química
Cynodon/crescimento & desenvolvimento
Larva/crescimento & desenvolvimento
Larva/microbiologia
Larva/fisiologia
Mariposas/crescimento & desenvolvimento
Oviposição
Paenibacillus/química
Pupa/crescimento & desenvolvimento
Pupa/microbiologia
Pupa/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx102


  6 / 703 MEDLINE  
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[PMID]:28829031
[Au] Autor:Wasoontharawat M; Phongsopitanun W; Siriudom S; Tanasupawat S
[Ad] Endereço:1​Department of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand.
[Ti] Título:Paenibacillus aurantiacus sp. nov., isolated from ant nest soil.
[So] Source:Int J Syst Evol Microbiol;67(9):3226-3230, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-positive, rod-shaped and endospore-forming bacterium, strain RC11T, isolated from ant nest soil collected from Udon Thani Province, Thailand was characterized in a taxonomic study based on a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain RC11T was affiliated to the genus Paenibacillus and was closely related to Paenibacillus phyllosphaerae LMG 22192T with 98.1 % sequence similarity. Strain RC11T contained meso-diaminopimelic in the cell-wall peptidoglycan. The major menaquinone was MK-7. Anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and C16 : 0 were the predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. The DNA G+C content was 57.9 mol%. In addition, strain RC11T and P. phyllosphaerae LMG 22192T showed a low level of DNA-DNA relatedness (15.6-33.2 %). Therefore, strain RC11T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus aurantiacus sp. nov. is proposed. The type strain is RC11T (=KCTC 33816T=LMG 29659T=TISTR 2452T).
[Mh] Termos MeSH primário: Formigas
Paenibacillus/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
Parede Celular/química
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Paenibacillus/genética
Paenibacillus/isolamento & purificação
Peptidoglicano/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (RNA, Ribosomal, 16S); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002088


  7 / 703 MEDLINE  
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[PMID]:28768765
[Au] Autor:Hino T; Hamamoto H; Suzuki H; Yagi H; Ohshiro T; Nagano S
[Ad] Endereço:From the Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyamacho-minami, Tottori 680-8552, Japan.
[Ti] Título:Crystal structures of TdsC, a dibenzothiophene monooxygenase from the thermophile sp. A11-2, reveal potential for expanding its substrate selectivity.
[So] Source:J Biol Chem;292(38):15804-15813, 2017 Sep 22.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sulfur compounds in fossil fuels are a major source of environmental pollution, and microbial desulfurization has emerged as a promising technology for removing sulfur under mild conditions. The enzyme TdsC from the thermophile sp. A11-2 is a two-component flavin-dependent monooxygenase that catalyzes the oxygenation of dibenzothiophene (DBT) to its sulfoxide (DBTO) and sulfone (DBTO ) during microbial desulfurization. The crystal structures of the apo and flavin mononucleotide (FMN)-bound forms of DszC, an ortholog of TdsC, were previously determined, although the structure of the ternary substrate-FMN-enzyme complex remains unknown. Herein, we report the crystal structures of the DBT-FMN-TdsC and DBTO-FMN-TdsC complexes. These ternary structures revealed many hydrophobic and hydrogen-bonding interactions with the substrate, and the position of the substrate could reasonably explain the two-step oxygenation of DBT by TdsC. We also determined the crystal structure of the indole-bound enzyme because TdsC, but not DszC, can also oxidize indole, and we observed that indole binding did not induce global conformational changes in TdsC with or without bound FMN. We also found that the two loop regions close to the FMN-binding site are disordered in apo-TdsC and become structured upon FMN binding. Alanine substitutions of Tyr-93 and His-388, which are located close to the substrate and FMN bound to TdsC, significantly decreased benzothiophene oxygenation activity, suggesting their involvement in supplying protons to the active site. Interestingly, these substitutions increased DBT oxygenation activity by TdsC, indicating that expanding the substrate-binding site can increase the oxygenation activity of TdsC on larger sulfur-containing substrates, a property that should prove useful for future microbial desulfurization applications.
[Mh] Termos MeSH primário: Oxirredutases/química
Oxirredutases/metabolismo
Paenibacillus/enzimologia
[Mh] Termos MeSH secundário: Domínio Catalítico
Cristalografia por Raios X
Mononucleotídeo de Flavina/metabolismo
Indóis/metabolismo
Modelos Moleculares
Mutação
Oxirredutases/genética
Especificidade por Substrato
Tiofenos/química
Tiofenos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoles); 0 (Thiophenes); 7N464URE7E (Flavin Mononucleotide); EC 1.- (Oxidoreductases); EC 1.8.2.- (dibenzothiophene monooxygenase); Z3D4AJ1R48 (dibenzothiophene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.788513


  8 / 703 MEDLINE  
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[PMID]:28699880
[Au] Autor:Im WT; Yi KJ; Lee SS; Moon HI; Jeon CO; Kim DW; Kim SK
[Ad] Endereço:1​Department of Biotechnology, Hankyoung National University, 327 Chungang-no Anseong-si, Kyonggi-do 17579, Republic of Korea.
[Ti] Título:Paenibacillus konkukensis sp. nov., isolated from animal feed.
[So] Source:Int J Syst Evol Microbiol;67(7):2343-2348, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146T, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillusyunnanensis JCM 30953T (98.6 %), Paenibacillusvulneris CCUG 53270T (98.0 %) and Paenibacilluschinjuensis DSM 15045T (96.9 %). Cells were non-motile, endospore-forming and formed milky colonies on NA and R2A agar media. Growth of strain SK-3146T occurred at temperatures of 18-45 °C, at pH 6.0-9.5 and between 0.5-3.0 % NaCl (w/v). The major menaquinone was MK-7, with lesser amounts of MK-6 present. The cell wall peptidoglycan of strain SK-3146T contained meso-diaminopimelic acid. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 53.8 mol% and the DNA-DNA hybridization relatedness values between strain SK-3146T and P.yunnanensis JCM 30953T and P.vulneris CCUG 53270T were 26.13±0.8 % and 38.7±0.6 %, respectively. The phenotypic, phylogenetic and chemotaxonomic results indicate that strain SK-3146T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus konkukensis sp. nov. is proposed. The type strain is SK-3146T (=KACC 18876T=LMG 29568T).
[Mh] Termos MeSH primário: Ração Animal/microbiologia
Paenibacillus/classificação
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Parede Celular/química
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Paenibacillus/genética
Paenibacillus/isolamento & purificação
Peptidoglicano/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 583-93-7 (Diaminopimelic Acid); 71ANL51TLA (menaquinone 6); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001955


  9 / 703 MEDLINE  
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[PMID]:28699855
[Au] Autor:Menéndez E; Flores-Félix JD; Mulas R; Andrés FG; Fernández-Pascual M; Peix A; Velázquez E
[Ad] Endereço:1​Departamento de Microbiología y Genética, Universidad de Salamanca and Instituto Hispanoluso de Investigaciones Agrarias (CIALE), Salamanca, Spain.
[Ti] Título:Paenibacillus tritici sp. nov., isolated from wheat roots.
[So] Source:Int J Syst Evol Microbiol;67(7):2312-2316, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bacterial strain designated RTAE36T was isolated from wheat roots in northern Spain. Phylogenetic analyses based on 16S rRNA gene sequence placed the isolate into the genus Paenibacillus with its closest relative being Paenibacillus borealis DSM 13188T with 97.7 % sequence similarity. Cells of the isolate were facultatively anaerobic, Gram-stain-positive, motile and sporulating rods. Catalase and oxidase were positive. Gelatin, casein and starch were not hydrolysed. Growth was supported by many carbohydrates and organic acids as carbon sources. MK-7 was the only menaquinone detected, and anteiso-C15 : 0, C16 : 0, iso-C14 : 0 and iso-C16 : 0 were the major fatty acids. The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid, two unidentified phospholipids, three unidentified phosphoaminolipids, one unidentified glycolipid and one unidentified lipid. meso-Diaminopimelic acid was detected in the cell-wall peptidoglycan. Strains RTAE36T and P. borealis DSM 13188T had an mean DNA-DNA relatedness of 39 % and differed in several phenotypic and chemotaxonomic characteristics, confirming that strain RTAE36T should be considered as a representative of a novel species of the genus Paenibacillus, for which the name Paenibacillus tritici sp. nov. is proposed. The type strain is RTAE36T (=LMG 29502T=CECT 9125T).
[Mh] Termos MeSH primário: Filogenia
Raízes de Plantas/microbiologia
Triticum/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Parede Celular/química
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Glicolipídeos/química
Hibridização de Ácido Nucleico
Paenibacillus/classificação
Paenibacillus/genética
Paenibacillus/isolamento & purificação
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espanha
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 583-93-7 (Diaminopimelic Acid); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001949


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[PMID]:28698247
[Au] Autor:Fujimoto Z; Suzuki N; Kishine N; Ichinose H; Momma M; Kimura A; Funane K
[Ad] Endereço:Advanced Analysis Center, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan zui@affrc.go.jp funane@affrc.go.jp.
[Ti] Título:Carbohydrate-binding architecture of the multi-modular α-1,6-glucosyltransferase from sp. 598K, which produces α-1,6-glucosyl-α-glucosaccharides from starch.
[So] Source:Biochem J;474(16):2763-2778, 2017 Aug 07.
[Is] ISSN:1470-8728
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:sp. 598K α-1,6-glucosyltransferase (Ps6TG31A), a member of glycoside hydrolase family 31, catalyzes exo-α-glucohydrolysis and transglucosylation and produces α-1,6-glucosyl-α-glucosaccharides from α-glucan via its disproportionation activity. The crystal structure of Ps6TG31A was determined by an anomalous dispersion method using a terbium derivative. The monomeric Ps6TG31A consisted of one catalytic (ß/α) -barrel domain and six small domains, one on the N-terminal and five on the C-terminal side. The structures of the enzyme complexed with maltohexaose, isomaltohexaose, and acarbose demonstrated that the ligands were observed in the catalytic cleft and the sugar-binding sites of four ß-domains. The catalytic site was structured by a glucose-binding pocket and an aglycon-binding cleft built by two sidewalls. The bound acarbose was located with its non-reducing end pseudosugar docked in the pocket, and the other moieties along one sidewall serving three subsites for the α-1,4-glucan. The bound isomaltooligosaccharide was found on the opposite sidewall, which provided the space for the acceptor molecule to be positioned for attack of the catalytic intermediate covalent complex during transglucosylation. The N-terminal domain recognized the α-1,4-glucan in a surface-binding mode. Two of the five C-terminal domains belong to the carbohydrate-binding modules family 35 and one to family 61. The sugar complex structures indicated that the first family 35 module preferred α-1,6-glucan, whereas the second family 35 module and family 61 module preferred α-1,4-glucan. Ps6TG31A appears to have enhanced transglucosylation activity facilitated by its carbohydrate-binding modules and substrate-binding cleft that positions the substrate and acceptor sugar for the transglucosylation.
[Mh] Termos MeSH primário: Acarbose/metabolismo
Proteínas de Bactérias/metabolismo
Glucosiltransferases/metabolismo
Oligossacarídeos/metabolismo
Paenibacillus/enzimologia
[Mh] Termos MeSH secundário: Acarbose/química
Apoenzimas/química
Apoenzimas/genética
Apoenzimas/metabolismo
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Sítios de Ligação
Biocatálise
Configuração de Carboidratos
Domínio Catalítico
Cristalização
Cristalografia por Raios X
Dimerização
Glucosiltransferases/química
Glucosiltransferases/genética
Indicadores e Reagentes/química
Ligantes
Oligossacarídeos/química
Conformação Proteica
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/química
Térbio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoenzymes); 0 (Bacterial Proteins); 0 (Indicators and Reagents); 0 (Ligands); 0 (Oligosaccharides); 0 (Recombinant Proteins); 06SSF7P179 (Terbium); 34620-77-4 (maltohexaose); 6175-02-6 (isomaltohexose); 804HI855F8 (terbium chloride); EC 2.4.1.- (Glucosyltransferases); T58MSI464G (Acarbose)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1042/BCJ20170152



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