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  1 / 193 MEDLINE  
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[PMID]:28271852
[Au] Autor:Zhang Y; Li D; Lv J; Li Q; Kong C; Luo Y
[Ad] Endereço:Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
[Ti] Título:Effect of cinnamon essential oil on bacterial diversity and shelf-life in vacuum-packaged common carp (Cyprinus carpio) during refrigerated storage.
[So] Source:Int J Food Microbiol;249:1-8, 2017 May 16.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study investigated the effect of cinnamon essential oil on the quality of vacuum-packaged common carp (Cyprinus carpio) fillets stored at 4±1°C in terms of sensory scores, physicochemical characteristics (total volatile basic nitrogen (TVB-N), biogenic amines, and color), and presence of spoilage microbiota. A total of 290,753 bacterial sequences and 162 different genera belonging to 14 phyla were observed by a high-throughput sequencing technique targeting the V3-V4 region of 16S rDNA, which showed a more comprehensive estimate of microbial diversity in carp samples compared with microbial enumeration. Before storage, Macrococcus and Aeromonas were the prevalent populations in the control samples, but cinnamon essential oil decreased the relative abundance of Macrococcus in the treated samples. Variability in the predominant microbiota in different samples during chilled storage was observed. Aeromonas followed by Lactococcus were the major contaminants in the spoiled control samples. Microbial enumeration also observed relatively higher counts of Aeromonas than other spoilage microorganisms. Compared with the control samples, cinnamon essential oil inhibited the growth of Aeromonas and Lactococcus were the predominant components in the treated samples on day 10; plate counts also revealed a relatively high level of lactic acid bacteria during refrigerated storage. However, there were no significant differences (P>0.05) in the composition of dominant microbiota between these two treatments at the end of the shelf-life. Furthermore, cinnamon essential oil treatment was more effective in inhibiting the increase of TVB-N and the accumulation of biogenic amines (especially for putrescine and cadaverine levels). Based primarily on sensory analysis, the use of cinnamon essential oil extended the shelf-life of vacuum-packaged common carp fillets by about 2days.
[Mh] Termos MeSH primário: Cadaverina/farmacologia
Conservação de Alimentos/métodos
Armazenamento de Alimentos/métodos
Óleos Voláteis/farmacologia
Putrescina/farmacologia
Alimentos Marinhos/microbiologia
[Mh] Termos MeSH secundário: Aeromonas/efeitos dos fármacos
Aeromonas/isolamento & purificação
Animais
Carpas
Cinnamomum zeylanicum/metabolismo
Microbiologia de Alimentos
Embalagem de Alimentos/métodos
Seres Humanos
Lactococcus/efeitos dos fármacos
Lactococcus/isolamento & purificação
Microbiota/efeitos dos fármacos
Tipagem Molecular
Nitrogênio/análise
RNA Ribossômico 16S/genética
Refrigeração
Staphylococcaceae/efeitos dos fármacos
Staphylococcaceae/isolamento & purificação
Vácuo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile); 0 (RNA, Ribosomal, 16S); L90BEN6OLL (Cadaverine); N762921K75 (Nitrogen); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE


  2 / 193 MEDLINE  
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[PMID]:28144940
[Au] Autor:Matthews S; Tee KL; Rattray NJ; McLean KJ; Leys D; Parker DA; Blankley RT; Munro AW
[Ad] Endereço:Manchester Institute of Biotechnology, Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), School of Chemistry, The University of Manchester, UK.
[Ti] Título:Production of alkenes and novel secondary products by P450 OleT using novel H O -generating fusion protein systems.
[So] Source:FEBS Lett;591(5):737-750, 2017 Mar.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Jeotgalicoccus sp. 8456 OleT (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H O ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase (AldO) is linked to OleT . AldO oxidizes polyols (including glycerol), generating H O as a coproduct and facilitating its use for efficient OleT -dependent fatty acid decarboxylation. AldO activity is regulatable by polyol substrate titration, enabling control over H O supply to minimize oxidative inactivation of OleT and prolong activity for increased alkene production. We also use these fusion systems to generate novel products from secondary turnover of 2-OH and 3-OH myristic acid primary products, expanding the catalytic repertoire of OleT .
[Mh] Termos MeSH primário: Oxirredutases do Álcool/metabolismo
Alcenos/metabolismo
Sistema Enzimático do Citocromo P-450/metabolismo
Ácidos Graxos/metabolismo
Peróxido de Hidrogênio/metabolismo
Microbiologia Industrial
Proteínas Recombinantes de Fusão/metabolismo
[Mh] Termos MeSH secundário: Oxirredutases do Álcool/genética
Biocatálise
Biocombustíveis
Sistema Enzimático do Citocromo P-450/genética
Descarboxilação
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Engenharia Metabólica
Ácido Mirístico/metabolismo
Oxirredução
Proteínas Recombinantes de Fusão/genética
Staphylococcaceae/enzimologia
Staphylococcaceae/genética
Streptomyces coelicolor/enzimologia
Streptomyces coelicolor/genética
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Alkenes); 0 (Biofuels); 0 (Fatty Acids); 0 (Recombinant Fusion Proteins); 0I3V7S25AW (Myristic Acid); 9035-51-2 (Cytochrome P-450 Enzyme System); BBX060AN9V (Hydrogen Peroxide); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.99.- (alditol oxidase (FAD), Streptomyces coelicolor)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170202
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12581


  3 / 193 MEDLINE  
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[PMID]:28053093
[Au] Autor:Matthews S; Belcher JD; Tee KL; Girvan HM; McLean KJ; Rigby SE; Levy CW; Leys D; Parker DA; Blankley RT; Munro AW
[Ad] Endereço:From the Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, Manchester M1 7DN, United Kingdom.
[Ti] Título:Catalytic Determinants of Alkene Production by the Cytochrome P450 Peroxygenase OleT .
[So] Source:J Biol Chem;292(12):5128-5143, 2017 Mar 24.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The sp. peroxygenase cytochrome P450 OleT (CYP152L1) is a hydrogen peroxide-driven oxidase that catalyzes oxidative decarboxylation of fatty acids, producing terminal alkenes with applications as fine chemicals and biofuels. Understanding mechanisms that favor decarboxylation over fatty acid hydroxylation in OleT could enable protein engineering to improve catalysis or to introduce decarboxylation activity into P450s with different substrate preferences. In this manuscript, we have focused on OleT active site residues Phe , His , and Arg to interrogate their roles in substrate binding and catalytic activity. His is a potential proton donor to reactive iron-oxo species during substrate decarboxylation. The H85Q mutant substitutes a glutamine found in several peroxygenases that favor fatty acid hydroxylation. H85Q OleT still favors alkene production, suggesting alternative protonation mechanisms. However, the mutant undergoes only minor substrate binding-induced heme iron spin state shift toward high spin by comparison with WT OleT , indicating the key role of His in this process. Phe interacts with His , and Phe mutants showed diminished affinity for shorter chain (C10-C16) fatty acids and weak substrate-induced high spin conversion. F79A OleT is least affected in substrate oxidation, whereas the F79W/Y mutants exhibit lower stability and cysteine thiolate protonation on reduction. Finally, Arg is crucial for binding the substrate carboxylate, and R245E/L mutations severely compromise activity and heme content, although alkene products are formed from some substrates, including stearic acid (C18:0). The results identify crucial roles for the active site amino acid trio in determining OleT catalytic efficiency in alkene production and in regulating protein stability, heme iron coordination, and spin state.
[Mh] Termos MeSH primário: Alcenos/metabolismo
Sistema Enzimático do Citocromo P-450/metabolismo
Peroxidases/metabolismo
Staphylococcaceae/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Domínio Catalítico
Cristalografia por Raios X
Sistema Enzimático do Citocromo P-450/química
Sistema Enzimático do Citocromo P-450/genética
Ácidos Graxos/metabolismo
Hidroxilação
Modelos Moleculares
Mutação
Peroxidases/química
Peroxidases/genética
Alinhamento de Sequência
Staphylococcaceae/química
Staphylococcaceae/genética
Staphylococcaceae/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkenes); 0 (Fatty Acids); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.11.1.- (Peroxidases); EC 1.11.1.- (cytochrome P-450 peroxygenase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.762336


  4 / 193 MEDLINE  
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[PMID]:28040597
[Au] Autor:Hassan SSU; Shaikh AL
[Ad] Endereço:Ocean College, Zhejiang University, Hangzhou 310058, China. Electronic address: hassan13@zju.edu.cn.
[Ti] Título:Marine actinobacteria as a drug treasure house.
[So] Source:Biomed Pharmacother;87:46-57, 2017 Mar.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Marine actinobacteria have been considered as a gold mine with respect to great potential regarding their secondary metabolites. Most of the researches have been conducted on actinobacteria's derived secondary metabolites to examine its pharmacological properties. Actinobacteria have a potential to provide future drugs against crucial diseases, such as drug-resistance bacteria, cancer, a range of viral illnesses, malaria, several infections and inflammations. Although, the mode of action of many bio molecules are still untapped, for a tangible number of compounds by which they interfere with human pathogenesis are reported here with detailed diagrammed illustrations. This knowledge is one of the basic vehicles to be known especially for transforming bio medicinal molecules to medicines. Actinobacteria produce a different kind of biochemical substances with numerous carbon skeletons, which have been found to be the main component interfering with human pathogenesis at different sites. Different diseases have the capability to fight at different sites inside the body can lead to a new wave of increasing the chances to produce targeted medicines.
[Mh] Termos MeSH primário: Actinobacteria/fisiologia
Produtos Biológicos/farmacologia
Descoberta de Drogas/tendências
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/farmacologia
Anti-Inflamatórios/uso terapêutico
Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Antineoplásicos/uso terapêutico
Produtos Biológicos/isolamento & purificação
Produtos Biológicos/uso terapêutico
Seres Humanos
Staphylococcaceae/efeitos dos fármacos
Staphylococcaceae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antineoplastic Agents); 0 (Biological Products)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170710
[Lr] Data última revisão:
170710
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


  5 / 193 MEDLINE  
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[PMID]:27902236
[Au] Autor:Li Y; Wang SK; Xue H; Chang JP; Guo LM; Yang XQ
[Ad] Endereço:1​The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
[Ti] Título:Corticicoccus populi gen. nov., sp. nov., a member of the family Staphylococcaceae, isolated from symptomatic bark of Populus × euramericana canker.
[So] Source:Int J Syst Evol Microbiol;67(4):789-794, 2017 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two Gram-stain-positive, aerobic, non-motile, bacterial strains were isolated from symptomatic bark tissue of Populus × euramericana canker. The isolates were able to grow between 10 and 37 °C, at pH 6-10 and with 0-4 % (w/v) NaCl, with optimal growth at 28-30 °C, pH 7.0-8.0 and with 2 % (w/v) NaCl The strains were found to be oxidase and catalase positive. The menaquinone of strain 26D10-3-4T was MK-7 and the peptidoglycan type A3α based on l-Lys-Gly3-?Ala. The polar lipid profiles of strain 26D10-3-4T showed diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and three unidentified glycolipids, and the major fatty acids found were anteiso-C15 : 0, iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0. The DNA G+C content was 38.2 mol%. The two novel isolates shared the highest 16S rRNA gene sequence similarity with Salinicoccus qingdaonensis ZXM223T (95.0 %). Based on phenotypic and genotypic characteristics, these two strains represent a novel species of a new genus of the family Staphylococcaceae; the name Corticicoccus populi gen. nov., sp. nov. is proposed. The type strain of the type species is 26D10-3-4T (=CFCC 12725T=KCTC 33575T). An additional strain of the species is 9-4-1.
[Mh] Termos MeSH primário: Filogenia
Casca de Planta/microbiologia
Doenças das Plantas/microbiologia
Populus/microbiologia
Staphylococcaceae/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Parede Celular/química
China
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Staphylococcaceae/genética
Staphylococcaceae/isolamento & purificação
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001602


  6 / 193 MEDLINE  
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[PMID]:27789876
[Au] Autor:Heß S; Lüddeke F; Gallert C
[Ad] Endereço:Faculty of Technology, Microbiology-Biotechnology, University of Applied Science Emden/Leer, Constantiaplatz 4, Emden 26723, Germany E-mail: stefanie.hess@hs-emden-leer.de.
[Ti] Título:Concentration of facultative pathogenic bacteria and antibiotic resistance genes during sewage treatment and in receiving rivers.
[So] Source:Water Sci Technol;74(8):1753-1763, 2016 Oct.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Whereas the hygienic condition of drinking and bathing water by law must be monitored by culture-based methods, for quantification of microbes and antibiotic resistance in soil or the aquatic environment, often molecular genetic assays are used. For comparison of both methods, knowledge of their correlation is necessary. Therefore the population of total bacteria, Escherichia coli, enterococci and staphylococci during sewage treatment and in receiving river water was compared by agar plating and quantitative polymerase chain reaction (qPCR) assays. In parallel, all samples were investigated for clinically relevant antibiotic resistance genes. Whereas plating and qPCR data for total bacteria correlated well in sewage after primary treatment, qPCR data of river water indicated higher cell numbers for E. coli. It is unknown if these cells are 'only' not growing under standard conditions or if they are dead. Corresponding to the amount of non-culturable cells, the 'breakpoints' for monitoring water quality should be adapted. The abundances of clinically relevant antibiotic resistance genes in river water were in the same order of magnitude or even higher than in treated sewage. For estimation of the health risk it is important to investigate which species carry respective genes and whether these genes are disseminated via gene transfer.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias/isolamento & purificação
Farmacorresistência Bacteriana/genética
Rios/microbiologia
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Bactérias/genética
Enterococcus/genética
Enterococcus/isolamento & purificação
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Alemanha
Staphylococcaceae/genética
Staphylococcaceae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Sewage)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170525
[Lr] Data última revisão:
170525
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


  7 / 193 MEDLINE  
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[PMID]:27381021
[Au] Autor:Srinivas A; Divyasree B; Tushar L; Suresh G; Sasikala C; Ramana CV
[Ad] Endereço:1​Bacterial Discovery Laboratory, Center for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad 500085, India.
[Ti] Título:Salinicoccus amylolyticus sp. nov., isolated from a saltern.
[So] Source:Int J Syst Evol Microbiol;66(10):3814-3820, 2016 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-positive coccus, strain JC304T, was isolated from a saltern of Nari along the Bhavnagar Coast, Gujarat, India. The 16S rRNA gene sequence analysis and sequence comparison data indicated that JC304T represented a member of the genus Salinicoccus and was most closely related to Salinicoccus roseus 9T (99.6 %), Salinicoccus luteus YIM 70202T (97.0 %), Salinicoccus hispanicus J-82T (97.0 %) and the remaining species of the genus Salinicoccus (<97 %). Genome relatedness based on DNA-DNA hybridization of JC304T with the type strains of the most closely related species was less than 46 % and the ΔTmwas >5 °C indicating that the strain represents a novel species of the genus Salinicoccus. Independent and concatenated phylogenetic analysis of recA/fusA gene translated product showed a clear distinction of JC304T from its phylogenetic neighbors. Diphosphotidylglycerol, phosphatidylglycerol, an unidentified glycolipid and three unidentified lipids (L1, L2 and L3) were the polar lipids of JC304T. Iso-C15 : 0 and anteiso-C15 : 0 were the major (>10 %) fatty acids in strain JC304T. The cell-wall amino acids were l-lysine and d-glycine. Hopanoids were not detected. The major isoprenoid quinone was menaquinone (MK-6). The DNA G+C content of JC304T was 48 mol%. On the basis of physiological, genotypic, phylogenetic and chemotaxonomic analyses, strain JC304T is considered to represent a novel species of the genus Salinicoccus, for which the name Salinicoccusamylolyticus sp. nov. is proposed. The type strain is JC304T (=KCTC 33661T=LMG 28757T).
[Mh] Termos MeSH primário: Filogenia
Salinidade
Staphylococcaceae/classificação
[Mh] Termos MeSH secundário: Aminoácidos/química
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Índia
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Staphylococcaceae/genética
Staphylococcaceae/isolamento & purificação
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 71ANL51TLA (menaquinone 6)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160707
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001270


  8 / 193 MEDLINE  
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[PMID]:27311112
[Au] Autor:Zhong N; Zhao M; Zhong L; Liao Q; Zhu X; Luo B; Li Y
[Ad] Endereço:Chongqing Key Laboratory of Fiber Optic Sensor and Photodetector, Chongqing Key Laboratory of Modern Photoelectric Detection Technology and Instrument, Chongqing University of Technology, Chongqing 400054, China. Electronic address: zhongnianbing@163.com.
[Ti] Título:A high-sensitivity fiber-optic evanescent wave sensor with a three-layer structure composed of Canada balsam doped with GeO2.
[So] Source:Biosens Bioelectron;85:876-882, 2016 Nov 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this paper, we present a high-sensitivity polymer fiber-optic evanescent wave (FOEW) sensor with a three-layer structure that includes bottom, inter-, and surface layers in the sensing region. The bottom layer and inter-layer are POFs composed of standard cladding and the core of the plastic optical fiber, and the surface layer is made of dilute Canada balsam in xylene doped with GeO2. We examine the morphology of the doped GeO2, the refractive index and composition of the surface layer and the surface luminous properties of the sensing region. We investigate the effects of the content and morphology of the GeO2 particles on the sensitivity of the FOEW sensors by using glucose solutions. In addition, we examine the response of sensors incubated with staphylococcal protein A plus mouse IgG isotype to goat anti-mouse IgG solutions. Results indicate very good sensitivity of the three-layer FOEW sensor, which showed a 3.91-fold improvement in the detection of the target antibody relative to a conventional sensor with a core-cladding structure, and the novel sensor showed a lower limit of detection of 0.2ng/l and a response time around 320s. The application of this high-sensitivity FOEW sensor can be extended to biodefense, disease diagnosis, biomedical and biochemical analysis.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/instrumentação
Tecnologia de Fibra Óptica/instrumentação
Germânio/química
Fibras Ópticas
[Mh] Termos MeSH secundário: Animais
Técnicas Biossensoriais/métodos
Desenho de Equipamento
Tecnologia de Fibra Óptica/métodos
Glucose/análise
Cabras
Imunoglobulina G/análise
Camundongos
Refratometria
Staphylococcaceae/química
Proteína Estafilocócica A/análise
Xilenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin G); 0 (Staphylococcal Protein A); 0 (Xylenes); 00072J7XWS (Germanium); 5O6CM4W76A (germanium oxide); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160617
[St] Status:MEDLINE


  9 / 193 MEDLINE  
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[PMID]:27282768
[Au] Autor:Glaeser SP; Kleinhagauer T; Jäckel U; Klug K; Kämpfer P
[Ad] Endereço:1​Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
[Ti] Título:Jeotgalicoccus schoeneichii sp. nov. isolated from exhaust air of a pig barn.
[So] Source:Int J Syst Evol Microbiol;66(9):3503-3508, 2016 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-staining-positive, non-motile, non-spore-forming, coccus (strain 140805-STR-02T) was isolated from exhaust air of a pig barn on Columbia Blood Agar Base (Oxoid) supplemented with 5 % defibrinated horse blood, Streptococcus selective supplement and 0.5 mg erythromycin l-1. The strains shared high 16S rRNA gene sequence similarity to Jeotgalicoccus pinnipedialis (98.6 %) but only a maximum of 94 % sequence similarity to all other species of the genus Jeotgalicoccus. DNA-DNA hybridisation values between strain 140805-STR-02T and J. pinnipedialis CIP 107946T were 60.3 % (reciprocal, 51.2 %). The quinone system of 140805-STR-02T contained predominantly menaquinone MK-7 and minor amounts of MK-6. The polar lipid profile of strain 140805-STR-02T contained the major compounds diphosphatidylglycerol and phosphatidylglycerol and four unidentified lipids present in minor to moderate amounts. In the polyamine pattern spermidine and spermine were predominant. The fatty acid profile comprising iso-C15 : 0 and anteiso-C15 : 0 as major fatty acids, and was in congruence with those reported for other species of the genus Jeotgalicoccus and thus supported the affiliation of strain 140805-STR-02T to this genus. The results of physiological and biochemical tests allowed a clear phenotypic differentiation of strain 140805-STR-02T from the most closely related species. Strain 140805-STR-02T represents a novel species, for which the names Jeotgalicoccus schoeneichii sp. nov. is proposed, with the type strain 140805-STR-02T (=LMG 29445T=CCM 8667T).
[Mh] Termos MeSH primário: Abrigo para Animais
Filogenia
Staphylococcaceae/classificação
Sus scrofa
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Alemanha
Hibridização de Ácido Nucleico
Poliaminas/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Staphylococcaceae/genética
Staphylococcaceae/isolamento & purificação
Suínos
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Polyamines); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 71ANL51TLA (menaquinone 6)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160611
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001230


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[PMID]:27272908
[Au] Autor:Jiang Z; Yuan CG; Xiao M; Tian XP; Khan IU; Kim CJ; Zhi XY; Li WJ
[Ad] Endereço:Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
[Ti] Título:Abyssicoccus albus gen. nov., sp. nov., a novel member of the family Staphylococcaceae isolated from marine sediment of the Indian Ocean.
[So] Source:Antonie Van Leeuwenhoek;109(8):1153-60, 2016 Aug.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A Gram-stain positive, aerobic, non-motile, asporogenous, coccoid shaped bacterium, designated YIM M12140(T), was isolated from a marine sediment sample collected from the Indian Ocean. Phylogenetic analysis showed that strain YIM M12140(T) forms a separate clade within the family Staphylococcaceae. Strain YIM M12140(T) shares high 16S rRNA gene sequence similarity with Macrococcus brunensis DSM 19358(T) (92.9 %). The isolate was found to grow at 0-10 % (w/v) NaCl (optimum, 2-3 %), pH 6.0-10.0 (optimum, pH 8.0) and temperature 5-40 °C (optimum, 28 °C). The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid and two unidentified polar lipids. The major cellular fatty acids of the strain were identified as anteiso-C15:0, -C17:0, iso-C16:0, anteiso-C19:0 and C20:0. The respiratory menaquinones were found to be MK-6 (94 %) and MK-7 (6 %). The cell wall amino acids were found to contain Lys, Ala, Glu, Gly, Asp, Ser and Thr. Whole cell sugars were identified as mannose, ribose, rhamnose, glucose, galactose and xylose. The G+C content of the genomic DNA of strain YIM M12140(T) was determined to be 42.4 mol %. Based on phenotypic, chemotaxonomic data and phylogenetic analysis, it is proposed that strain YIM M12140(T) represents a novel species of a new genus in the family Staphylococcaceae, for which the name Abyssicoccus albus gen. nov., sp. nov. is proposed. The type strain is YIM M12140(T) (= DSM 29158(T) = CCTCC AB 2014213(T)).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Staphylococcaceae/classificação
Staphylococcaceae/isolamento & purificação
[Mh] Termos MeSH secundário: Parede Celular/química
DNA Bacteriano/genética
DNA Ribossômico/genética
Ácidos Graxos/metabolismo
Oceano Índico
Peptidoglicano/metabolismo
Fosfolipídeos/metabolismo
Filogenia
Água do Mar/microbiologia
Análise de Sequência de DNA
Staphylococcaceae/genética
Staphylococcaceae/metabolismo
Vitamina K 2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phospholipids); 11032-49-8 (Vitamin K 2)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-016-0717-2



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