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[PMID]:29399876
[Au] Autor:Martínez-García S; Rodríguez-Martínez S; Cancino-Diaz ME; Cancino-Diaz JC
[Ad] Endereço:Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, Mexico.
[Ti] Título:Extracellular proteases of Staphylococcus epidermidis: roles as virulence factors and their participation in biofilm.
[So] Source:APMIS;126(3):177-185, 2018 Mar.
[Is] ISSN:1600-0463
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Staphylococci produce a large number of extracellular proteases, some of which are considered as potential virulence factors. Staphylococcus epidermidis is a causative agent of nosocomial infections in medical devices by the formation of biofilms. It has been proposed that proteases contribute to the different stages of biofilm formation. S. epidermidis secretes a small number of extracellular proteases, such as serine protease Esp, cysteine protease EcpA, and metalloprotease SepA that have a relatively low substrate specificity. Recent findings indicate a significant contribution of extracellular proteases in biofilm formation through the proteolytic inactivation of adhesion molecules. The objective of this work is to provide an overview of the current knowledge of S. epidermidis' extracellular proteases during pathogenicity, especially in the different stages of biofilm formation.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Biofilmes/crescimento & desenvolvimento
Cisteína Proteases/metabolismo
Metaloendopeptidases/metabolismo
Serina Proteases/metabolismo
Staphylococcus epidermidis/enzimologia
[Mh] Termos MeSH secundário: Moléculas de Adesão Celular/metabolismo
Infecção Hospitalar/microbiologia
Infecção Hospitalar/patologia
Seres Humanos
Infecções Estafilocócicas/microbiologia
Infecções Estafilocócicas/patologia
Staphylococcus epidermidis/metabolismo
Staphylococcus epidermidis/patogenicidade
Fatores de Virulência/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cell Adhesion Molecules); 0 (Virulence Factors); EC 3.4.- (Cysteine Proteases); EC 3.4.- (Serine Proteases); EC 3.4.24.- (Metalloendopeptidases); EC 3.4.24.- (SepA protein, Staphylococcus epidermidis)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180206
[St] Status:MEDLINE
[do] DOI:10.1111/apm.12805


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[PMID]:29442030
[Au] Autor:Resende AFC; Pereira AF; Moreira TP; Patrício PSO; Fialho SL; Cunha GMF; Silva-Cunha A; Magalhães JT; Silva GR
[Ti] Título:PLGA Implants containing vancomycin and dexamethasone: development, characterization and bactericidal effects.
[So] Source:Pharmazie;71(8):439-446, 2016 08 01.
[Is] ISSN:0031-7144
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Post-operative endophthalmitis is an infection and an inflammation of the eye following a surgical procedure. Its treatment is based on drug injections into the eye. However, this treatment can lead to ocular complications. Intraocular implants could substitute the conventional therapy. Poly(lactic-co-glycolic acid) (PLGA) implants comprising on vancomycin and dexamethasone were evaluated as drug delivery system to treat endophthalmitis after cataract surgery. Implants were characterized by drug content uniformity, Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Wide Angle X-ray Scattering (WAXS), Scanning Electron Microscopy (SEM) and in vitro drug release. The bactericidal effect of vancomycin, eluted from the implants, was demonstrated against Staphylococcus aureus and Staphylococcus epidermidis. The drugs were uniformly distributed in the polymer. The analytical techniques revealed the chemical integrity of the drugs incorporated into the polymer and the modification of dexamethasone semi-crystalline nature. Drugs were controlled released from implants; and the eluted vancomycin showed bactericidal effects. In conclusion, PLGA implants containing vancomycin and dexamethasone may represent a therapeutic alternative to treat post-operative endophthalmitis.
[Mh] Termos MeSH primário: Antibacterianos/administração & dosagem
Antibacterianos/uso terapêutico
Anti-Inflamatórios/administração & dosagem
Anti-Inflamatórios/uso terapêutico
Bactérias/efeitos dos fármacos
Dexametasona/administração & dosagem
Dexametasona/uso terapêutico
Portadores de Fármacos
Ácido Láctico
Ácido Poliglicólico
Infecção da Ferida Cirúrgica/prevenção & controle
Vancomicina/administração & dosagem
Vancomicina/uso terapêutico
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Implantes de Medicamento
Liberação Controlada de Fármacos
Endoftalmite/microbiologia
Endoftalmite/prevenção & controle
Seres Humanos
Testes de Sensibilidade Microbiana
Procedimentos Cirúrgicos Oftalmológicos
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus epidermidis/efeitos dos fármacos
Vancomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Inflammatory Agents); 0 (Drug Carriers); 0 (Drug Implants); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 6Q205EH1VU (Vancomycin); 7S5I7G3JQL (Dexamethasone)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1691/ph.2016.6009


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[PMID]:29361927
[Au] Autor:Lin Z; Feng X; Zheng L; Moonasar N; Shen L; Wu R; Chen F
[Ad] Endereço:The Eye Hospital, School of Ophthalmology and Optometry, Wenzhou Medical University, No. 270 West College Road, Wenzhou, Zhejiang, 325027, China.
[Ti] Título:Incidence of endophthalmitis after 23-gauge pars plana vitrectomy.
[So] Source:BMC Ophthalmol;18(1):16, 2018 Jan 23.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Endophthalmitis is a rare but severe complication following PPV. The incidence of endophthalmitis varies between 20-gauge, 23-gauge, and 25-gauge incisions. The incidence and clinical features of endophthalmitis after 23-gauge PPV in an eye hospital in China was reported in this study. METHODS: Data of the eyes that underwent 23-gauge PPV from January 2011 to December 2014 at the Eye Hospital of Wenzhou Medical University was retrospectively collected. All the information was obtained from the electronic medical system. The exclusion criteria included: (1) preoperative diagnosis of endophthalmitis; (2) history of vitrectomy; (3) intraocular surgery within 6 months; (4) history of ocular penetrating trauma; (5) sutures for any of the 3 sclerotomy incisions; (6) patients with cancer, acquired immune deficiency syndrome, or taking drugs that may influence the immune system. The diagnosis of endophthalmitis was based on clinical characteristics and/or culture results from an operative sample. RESULTS: Three thousand nine hundred seventy nine eyes that underwent 23-gauge PPV surgery were included in this study. Among these eyes, 3 eyes developed endophthalmitis after surgery, giving an incidence of 0.075% (3/3979). The period in which endophthalmitis developed ranged from 1 to 5 days post-operation. The visual acuity decreased to hand motions or light perception postoperatively. The culture of aqueous and vitreous of the 2 eyes revealed Staphylococcus epidermidis and enterococcus faecalis respectively, however was negative for the third eye. All 3 eyes had a favorable response to the treatment of vitreous tap and intravitreal antibiotics injection. Two eyes gained visual acuity of 0.05 and 0.5, respectively at the final visit. CONCLUSIONS: Endophthalmitis is a rare but sight-threatening complication after 23-gauge pars plana vitrectomy. The peak duration of onset was within 5 days post-operation, with gram positive cocci being the common pathogenic organism.
[Mh] Termos MeSH primário: Endoftalmite/epidemiologia
Infecções Oculares Bacterianas/epidemiologia
Microcirurgia/efeitos adversos
Doenças Retinianas/cirurgia
Infecções Estafilocócicas/epidemiologia
Infecção da Ferida Cirúrgica/epidemiologia
Vitrectomia/efeitos adversos
[Mh] Termos MeSH secundário: Adulto
Idoso
Humor Aquoso/microbiologia
China/epidemiologia
Endoftalmite/diagnóstico
Endoftalmite/microbiologia
Enterococcus faecalis/isolamento & purificação
Infecções Oculares Bacterianas/diagnóstico
Infecções Oculares Bacterianas/microbiologia
Feminino
Seguimentos
Infecções por Bactérias Gram-Positivas/diagnóstico
Infecções por Bactérias Gram-Positivas/epidemiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Seres Humanos
Incidência
Masculino
Microcirurgia/instrumentação
Meia-Idade
Estudos Retrospectivos
Infecções Estafilocócicas/diagnóstico
Infecções Estafilocócicas/microbiologia
Staphylococcus epidermidis/isolamento & purificação
Infecção da Ferida Cirúrgica/microbiologia
Ultrassonografia
Acuidade Visual
Vitrectomia/instrumentação
Corpo Vítreo/diagnóstico por imagem
Corpo Vítreo/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0678-5


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[PMID]:28554232
[Au] Autor:Guo T; Tan SB; Wang Y; Chang J
[Ad] Endereço:a School of Life Science and Engineering , Lanzhou University of Technology , Lanzhou , China.
[Ti] Título:Two new monoterpenoid glycosides from the fresh rhizome of Tongling White Ginger (Zingiber officinale).
[So] Source:Nat Prod Res;32(1):71-76, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two new monoterpenoid glycosides, trans-1,8-cineole-3,6-dihydroxy-3-O-ß-D-glucopyranoside (1), and 5,9-dihydroxy borneol 2-O-ß-D-glucopyranoside (2), together with four known monoterpenoid glycosides (3-6), were isolated from the water-soluble constituents of the fresh rhizome of Tongling White Ginger (Zingiber officinale). Their structures were decisively elucidated by spectroscopic analysis. In vitro tests for antimicrobial activity showed that compounds 1 and 3 possess significant activity against two Gram-positive organisms, Staphylococcus aureus and Staphylococcus epidermidis.
[Mh] Termos MeSH primário: Antibacterianos/química
Gengibre/química
Glicosídeos/química
Monoterpenos/química
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Glicosídeos/farmacologia
Espectroscopia de Ressonância Magnética
Testes de Sensibilidade Microbiana
Estrutura Molecular
Monoterpenos/farmacologia
Rizoma/química
Solubilidade
Espectrometria de Massas por Ionização por Electrospray
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus epidermidis/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Glycosides); 0 (Monoterpenes)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1333994


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[PMID]:27773522
[Au] Autor:Tamulaitis G; Venclovas C; Siksnys V
[Ad] Endereço:Institute of Biotechnology, Vilnius University, Sauletekio av. 7, Vilnius 10257, Lithuania.
[Ti] Título:Type III CRISPR-Cas Immunity: Major Differences Brushed Aside.
[So] Source:Trends Microbiol;25(1):49-61, 2017 Jan.
[Is] ISSN:1878-4380
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:For a long time the mechanism of immunity provided by the Type III CRISPR-Cas systems appeared to be inconsistent: the Type III-A Csm complex of Staphylococcus epidermidis was first reported to target DNA while Type III-B Cmr complexes were shown to target RNA. This long-standing conundrum has now been resolved by finding that the Type III CRISPR-Cas systems are both RNases and target RNA-activated DNA nucleases. The immunity is achieved by coupling binding and cleavage of RNA transcripts to the degradation of invading DNA. The base-pairing potential between the target RNA and the CRISPR RNA (crRNA) 5'-handle seems to play an important role in discriminating self and non-self nucleic acids; however, the detailed mechanism remains to be uncovered.
[Mh] Termos MeSH primário: Antibiose/genética
Sistemas CRISPR-Cas/genética
Proteínas de Ligação a DNA/genética
DNA/metabolismo
Proteínas de Ligação a RNA/genética
RNA/metabolismo
Staphylococcus epidermidis/genética
[Mh] Termos MeSH secundário: Sistemas CRISPR-Cas/fisiologia
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (RNA-Binding Proteins); 63231-63-0 (RNA); 9007-49-2 (DNA)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29242153
[Au] Autor:Raj R; Mitra S; Gopal B
[Ad] Endereço:Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
[Ti] Título:Characterization of Staphylococcus epidermidis Polynucleotide phosphorylase and its interactions with ribonucleases RNase J1 and RNase J2.
[So] Source:Biochem Biophys Res Commun;495(2):2078-2084, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polynucleotide phosphorylase catalyzes both 3'-5' exoribonuclease and polyadenylation reactions. The crystal structure of Staphylococcus epidermidis PNPase revealed a bound phosphate in the PH2 domain of each protomer coordinated by three adjacent serine residues. Mutational analysis suggests that phosphate coordination by these serine residues is essential to maintain the catalytic center in an active conformation. We note that PNPase forms a complex with RNase J1 and RNase J2 without substantially altering either exo-ribonuclease or polyadenylation activity of this enzyme. This decoupling of catalytic activity from protein-protein interactions suggests that association of these endo- or exo-ribonucleases with PNPase could be more relevant for cellular localization or concerted targeting of structured RNA for recycling.
[Mh] Termos MeSH primário: Simulação de Acoplamento Molecular
Nucleotidiltransferases/química
Nucleotidiltransferases/ultraestrutura
Ribonucleases/química
Ribonucleases/ultraestrutura
Staphylococcus epidermidis/enzimologia
[Mh] Termos MeSH secundário: Sítios de Ligação
Ativação Enzimática
Estabilidade Enzimática
Modelos Químicos
Complexos Multienzimáticos
Ligação Proteica
Conformação Proteica
Relação Estrutura-Atividade
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Multienzyme Complexes); EC 2.7.7.- (Nucleotidyltransferases); EC 2.7.7.- (polynucleotide pyrophosphorylase); EC 3.1.- (Ribonucleases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE


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[PMID]:29241121
[Au] Autor:Udayabhanu J; Kannan V; Tiwari M; Natesan G; Giovanni B; Perumal V
[Ad] Endereço:Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India.
[Ti] Título:Nanotitania crystals induced efficient photocatalytic color degradation, antimicrobial and larvicidal activity.
[So] Source:J Photochem Photobiol B;178:496-504, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Textile industries release tonnes of harmful toxic dyes into the environment, causing severe effects on living organisms, including humans. Mosquitoes vectors spread important diseases which cause millions of human deaths worldwide. To control mosquitoes a number of synthetic mosquitocidal agents have been employed but all these pesticides pose harmful effects to human health and non-target species and also led to resistance development in treated vectors. Microbial strains are also developing resistance to the available antibiotics, this currently represents a major public health challenge. The current study is focused on the green synthesis of titanium dioxide nanoparticles (TiO NPs) using aqueous leaf extracts of Euphorbia hirta. Results suggested an efficient remedy for the above mentioned problems using TiO NPs against the dye degradation, mosquito larvae and bacterial pathogens. The fabrication of TiO NPs was confirmed by UV-visible spectroscopy, the biomolecules involved in the synthesis process were evidenced by Fourier transform infra-red spectroscopy (FT-IR), the crystalline structure was observed by using X-ray powder diffraction (XRD) analysis. Spherical shaped TiO NPs were recorded using field emission scanning electron microscopy (FESEM). Energy dispersive X-ray spectroscopy (EDX) results showed the elemental composition of TiO NPs. Enhanced rate of photocatalytic dye degradation efficacy was recorded in in methylene blue (95.8%) followed by crystal violet (86.7%). Antibacterial activity assays indicated growth inhibition was highest in Staphylococcus epidermidis and Proteus vulgaris. The LC of TiO NPs and E. hirta extract on Aedes aegypti larvae were 13.2mg/l and 81.2mg/l, while on Culex quinquefasciatus they were 6.89mg/l and 46.1mg/l respectively. Overall, based on the results of the present study, the green engineered nanotitania could be considered as novel and promising photocatalytic, antibacterial, and mosquitocidal agent.
[Mh] Termos MeSH primário: Anti-Infecciosos/química
Corantes/química
Nanopartículas Metálicas/química
Titânio/química
[Mh] Termos MeSH secundário: Aedes/efeitos dos fármacos
Aedes/crescimento & desenvolvimento
Animais
Anti-Infecciosos/farmacologia
Compostos Azo/química
Catálise
Escherichia coli/efeitos dos fármacos
Euphorbia/química
Euphorbia/metabolismo
Química Verde
Larva/efeitos dos fármacos
Luz
Nanopartículas Metálicas/toxicidade
Azul de Metileno/química
Fotólise/efeitos dos fármacos
Fotólise/efeitos da radiação
Extratos Vegetais/química
Folhas de Planta/química
Folhas de Planta/metabolismo
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus epidermidis/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Azo Compounds); 0 (Coloring Agents); 0 (Plant Extracts); 15FIX9V2JP (titanium dioxide); 6B4TC34456 (methyl orange); D1JT611TNE (Titanium); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE


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[PMID]:28744944
[Au] Autor:Dodou HV; de Morais Batista AH; Sales GWP; de Medeiros SC; Rodrigues ML; Nogueira PCN; Silveira ER; Nogueira NAP
[Ad] Endereço:Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Brazil.
[Ti] Título:Violacein antimicrobial activity on Staphylococcus epidermidis and synergistic effect on commercially available antibiotics.
[So] Source:J Appl Microbiol;123(4):853-860, 2017 Oct.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The study aimed to assess whether violacein has antimicrobial activity on Staphylococcus epidermidis and synergistically modulates the action of commercially available antimicrobial drugs. METHODS AND RESULTS: Violacein showed excellent antimicrobial activity on biofilm-forming and nonbiofilm-forming S. epidermidis strains (ATCC 35984) (ATCC 12228), with bacteriostatic (MIC = 20 µg ml and 10 µg ml respectively) and bactericidal effects (MBC = 20 µg ml for both strains), observed in short periods of exposure. The violacein bactericidal concentration led to S. epidermidis death after 2-3 h of exposure. Additionally, violacein synergistically modulated the activity of different antimicrobial classes on S. epidermidis ATCC 12228 (81·8%; n = 9) and on S. epidermidis ATCC 35984 (54·5%; n = 6), reducing the MIC of these antibiotics by up to 16-fold. CONCLUSION: Violacein shows excellent antimicrobial activity on S. epidermidis strains. SIGNIFICANCE AND IMPACT OF THE STUDY: Violacein shows the potential for the development of a new drug for the treatment of infections caused by S. epidermidis.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Indóis/farmacologia
Staphylococcus epidermidis/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antibacterianos/economia
Biofilmes/efeitos dos fármacos
Sinergismo Farmacológico
Testes de Sensibilidade Microbiana
Staphylococcus epidermidis/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Indoles); QJH0DSQ3SG (violacein)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13547


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[PMID]:28458355
[Au] Autor:Ikeda R; Ogasawara Y; Takatori K; Ichikawa T; Nakajima M; Harigaya K; Watanabe M; Okudaira E; Yoshikawa H; Yanagisawa K
[Ad] Endereço:Department of Microbial Science and Host Defense, Meiji Pharmaceutical University.
[Ti] Título:Growth Inhibition of an Opportunistic Yeast Pathogen Trichosporon asahii by Staphylococcus epidermidis.
[So] Source:Biol Pharm Bull;40(5):693-697, 2017.
[Is] ISSN:1347-5215
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:In the co-culture of Staphylococcus epidermidis and Trichosporon asahii, a fungal pathogen, it was observed that live S. epidermidis inhibited the growth of T. asahii. Soluble active anti-T. asahii substances were speculated to be produced by S. epidermidis in culture medium. Using H- and C-NMR spectra and electron ionization-high resolution mass spectrometry (HR-negative-FAB-MS), we separated the active molecule and identified it as lactic acid. Commercially available L-lactic acid and D-lactic acid inhibited the growth of T. asahii. These results show that metabolites from bacterial populations are involved in the interactions of pathogenic fungi. The use of antibacterial agents to treat primary diseases could lead to the disruption of normal microbial communities and could cause opportunistic infections such as trichosporonosis.
[Mh] Termos MeSH primário: Staphylococcus epidermidis/metabolismo
Trichosporon/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Meios de Cultura
Ácido Láctico/química
Ácido Láctico/farmacologia
Espectroscopia de Ressonância Magnética
Espectrometria de Massas de Bombardeamento Rápido de Átomos
Staphylococcus epidermidis/química
Estereoisomerismo
Trichosporon/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 33X04XA5AT (Lactic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1248/bpb.b16-01000


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[PMID]:29388540
[Au] Autor:Taha M; Kohnen C; Mallya S; Kou Y; Zapata A; Ramirez-Arcos S
[Ad] Endereço:Canadian Blood Services, Ottawa, ON, Canada.
[Ti] Título:Comparative characterisation of the biofilm-production abilities of Staphylococcus epidermidis isolated from human skin and platelet concentrates.
[So] Source:J Med Microbiol;67(2):190-197, 2018 Feb.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Staphylococcus epidermidis is the predominant contaminant of platelet concentrates (PCs), a blood product used to treat patients with platelet deficiencies. This microorganism is able to form surface-attached aggregates (biofilms) in human skin. Herein, the abundance of S. epidermidis biofilm-producers in contaminated PCs compared to skin isolates was explored. Furthermore, the potential positive selection of S. epidermidis biofilm-producers during the blood donation process and PC manufacturing was investigated. METHODOLOGY: Twenty-four S. epidermidis isolates obtained from contaminated PCs and 48 S. epidermidis isolates obtained from the venipuncture area of human volunteers were compared for their ability to form biofilms in laboratory media and in PCs using a semi quantitative crystal violet assay. Also, the presence of the biofilm-associated icaA and icaD genes was assessed by PCR-amplification.Results/Key findings.Biofilm production in laboratory media showed a higher number of S. epidermidis biofilm-producers in the skin-derived group (43.7 %) compared to the PC-derived isolates (25 %). However, all skin and PC isolates formed biofilms in PCs. The prevalence of ica-positive biofilm-producer isolates was similar in PC and skin isolates (16.6 and 18.8 %, respectively). In contrast, the abundance of ica-negative biofilm-producers was lower in PC isolates compared to skin isolates (8.3 vs 25 %, respectively). CONCLUSION: Positive selection of S. epidermidis biofilm-producers during blood donation and PC manufacturing was not observed. Interestingly, ica-negative biofilm-producers seem to be negatively affected by skin disinfection, blood processing and PC storage. Furthermore, this study shows that S. epidermidis adopts a biofilm-forming phenotype in PCs regardless of its genetic background or origin.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Plaquetas/microbiologia
Pele/microbiologia
Staphylococcus epidermidis/fisiologia
[Mh] Termos MeSH secundário: DNA Bacteriano/genética
Feminino
Genótipo
Seres Humanos
Masculino
Fenótipo
Reação em Cadeia da Polimerase
Infecções Estafilocócicas/microbiologia
Staphylococcus epidermidis/classificação
Staphylococcus epidermidis/genética
Staphylococcus epidermidis/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000673



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