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[PMID]:28859149
[Au] Autor:Seng R; Kitti T; Thummeepak R; Kongthai P; Leungtongkam U; Wannalerdsakun S; Sitthisak S
[Ad] Endereço:Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.
[Ti] Título:Biofilm formation of methicillin-resistant coagulase negative staphylococci (MR-CoNS) isolated from community and hospital environments.
[So] Source:PLoS One;12(8):e0184172, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Methicillin-resistant coagulase negative staphylococci (MR-CoNS) are the major cause of infectious diseases because of their potential ability to form biofilm and colonize the community or hospital environments. This study was designed to investigate the biofilm producing ability, and the presence of mecA, icaAD, bap and fnbA genes in MR-CoNS isolates. The MR-CoNS used in this study were isolated from various samples of community environment and five wards of hospital environments, using mannitol salt agar (MSA) supplemented with 4 µg/ml of oxacillin. The specie level of Staphylococcus haemolyticus, Staphylococcus epidermidis, Staphylococcus hominis and Staphylococcus warneri was identified by specific primers of groESL (S. haemolyticus), rdr (S. epidermidis) and nuc (S. hominis and S. warneri). The remainder isolates were identified by tuf gene sequencing. Biofilm production was determined using Congo red agar (CRA) and Microtiter plate (MTP) assay. The mecA and biofilm associated genes (icaAD, fnbA and bap) were detected using PCR method. From the 558 samples from community and hospital environments, 292 MR-CoNS were isolated (41 from community environments, and 251 from hospital environments). S. haemolyticus (41.1%) and S. epidermidis (30.1%) were the predominant species in this study. Biofilm production was detected in 265 (90.7%) isolates by CRA, and 260 (88.6%) isolates were detected by MTP assay. The staphylococci isolates derived from hospital environments were more associated with biofilm production than the community-derived isolates. Overall, the icaAD and bap genes were detected in 74 (29.5%) and 14 (5.6%) of all isolates from hospital environments. When tested by MTP, the icaAD gene from hospital environment isolates was associated with biofilm biomass. No association was found between bap gene and biofilm formation. The MR-CoNS isolates obtained from community environments did not harbor the icaAD and bap genes. Conversely, fnbA gene presented in MR-CoNS isolated from both community and hospital environments. The high prevalence of biofilm producing MR-CoNS strains demonstrated in this study indicates the persisting ability in environments, and is useful in developing prevention strategies countering the spread of MR-CoNS.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Infecção Hospitalar/genética
Resistência a Meticilina/genética
Infecções Estafilocócicas/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Coagulase/genética
Infecção Hospitalar/microbiologia
Seres Humanos
Oxacilina/administração & dosagem
Proteínas de Ligação às Penicilinas/genética
Infecções Estafilocócicas/microbiologia
Staphylococcus epidermidis/genética
Staphylococcus epidermidis/crescimento & desenvolvimento
Staphylococcus haemolyticus/genética
Staphylococcus haemolyticus/crescimento & desenvolvimento
Staphylococcus hominis/genética
Staphylococcus hominis/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bap protein, Staphylococcus aureus); 0 (Coagulase); 0 (Penicillin-Binding Proteins); 0 (mecA protein, Staphylococcus aureus); UH95VD7V76 (Oxacillin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184172


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[PMID]:28700050
[Au] Autor:Soroush S; Jabalameli F; Taherikalani M; Eslampour MA; Beigverdi R; Emaneini M
[Ad] Endereço:Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
[Ti] Título:Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children.
[So] Source:Rev Soc Bras Med Trop;50(3):329-333, 2017 May-Jun.
[Is] ISSN:1678-9849
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION:: Methicillin resistant Staphylococcus hominis (MRSHo) has been recognized as an important human pathogen, particularly in immunocompromised patients. METHODS:: A total of 19 S. hominis isolates were collected from children at the Children's Medical Centre, Tehran, Iran, from March 2012 to February 2013. MRSHo susceptibility against 13 antimicrobial and 3 antiseptic agents was determined using disk diffusion (DAD) and minimum inhibitory concentration (MIC), respectively. All isolates were subjected to polymerase chain reaction (PCR) assay for 15 distinct resistance genes, staphylococcal cassette chromosome mec (SCCmec), and arginine catabolic mobile elements (ACMEs). Biofilm production of the isolates was determined using a colorimetric microtiter plate assay. RESULTS: : Of the 19 isolates, 16 were resistant to oxacillin and harbored mecA. High resistance was also observed against trimethoprim/sulfamethoxazole (81.2%). All MRSHo isolates were susceptible to the three disinfectants tested (Septicidine-PC, Septi turbo, and Sayacept-HP). In total, 15 (78.9%) isolates produced biofilms. Three isolates had SCCmec types (V and VIII), 13 were untypable (UT), and 5 had ACME type II. CONCLUSIONS:: The results indicate that MRSHo with high antibiotic resistance and unknown SCCmec might become a serious problem in the future for the treatment of patients such as children.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Biofilmes/crescimento & desenvolvimento
Cromossomos Bacterianos/genética
Resistência a Meticilina/genética
Infecções Estafilocócicas/microbiologia
Staphylococcus hominis/efeitos dos fármacos
[Mh] Termos MeSH secundário: Criança
DNA Bacteriano
Farmacorresistência Bacteriana
Seres Humanos
Irã (Geográfico)
Testes de Sensibilidade Microbiana
Staphylococcus hominis/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE


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[PMID]:27979420
[Au] Autor:Yan ST; Sun LC; Jia HB; Gao W; Yang JP; Zhang GQ
[Ad] Endereço:Department of Emergency Medicine, China-Japan Friendship Hospital, Beijing, China.
[Ti] Título:Procalcitonin levels in bloodstream infections caused by different sources and species of bacteria.
[So] Source:Am J Emerg Med;35(4):579-583, 2017 Apr.
[Is] ISSN:1532-8171
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The aim of this study was to evaluate procalcitonin (PCT) diagnostic accuracy in discriminating gram-negative (GN) from gram-positive (GP) bloodstream infections and determining the relationship between PCT levels, infection sites, and pathogen types. METHODS: Clinical and laboratory data were collected from patients with blood culture (BC)-positive sepsis between January 2014 and December 2015. PCT levels at different infection sites were compared, as was the presence of GN and GP bloodstream infection. A receiver operating characteristic (ROC) curve was generated to assess diagnostic accuracy. RESULTS: Of the 486 monomicrobial BCs, 254 (52.26%) were positive for GN bacteria (GNB), and 202 (42.18%) for GP bacteria (GPB). Median PCT levels were higher in BCs positive for GN (2.42ng/ml, IQR: 0.38-15.52) than in those positive for GPB (0.49ng/ml, IQR: 0.13-5.89) (P<0.001). In the ROC analysis to differentiate between GNB and GPB, the area under the curve was 0.628 (95% CI: 0.576-0.679). When the cutoffs for PCT were 10.335 and 15.000ng/ml, the specificity of GNB infection was 80.2% and 84.2%, respectively. PCT levels caused by GNB differed between Escherichia coli and Acinetobacter baumanni/Burkholderia cepacia, Klebsiella pneumonia and Acinetobacter baumanni. PCT levels caused by GPB differed between Staphylococcus epidermidis/Staphylococcus aureus and Staphylococcus hominis/Staphylococcus haemolyticus, Enterococcus faecium and Enterococcus faecalis/S.hominis/S. haemolyticus. Among patients with known infection sites, there were statistical differences in PCT levels between abdominal infection and pneumonia/infective endocarditis, urinary tract infection and pneumonia/catheter-related infection/infective endocarditis. CONCLUSION: PCT can distinguish between GNB and GPB infection, as well as between different bacterial species and infection sites.
[Mh] Termos MeSH primário: Bacteriemia/sangue
Calcitonina/sangue
Infecções Relacionadas a Cateter/sangue
Endocardite Bacteriana/sangue
Infecções por Bactérias Gram-Negativas/sangue
Infecções por Bactérias Gram-Positivas/sangue
Pneumonia Bacteriana/sangue
Infecções Urinárias/sangue
[Mh] Termos MeSH secundário: Infecções por Acinetobacter/sangue
Infecções por Acinetobacter/microbiologia
Acinetobacter baumannii
Idoso
Idoso de 80 Anos ou mais
Bacteriemia/microbiologia
Biomarcadores/sangue
Infecções por Burkholderia/sangue
Infecções por Burkholderia/microbiologia
Burkholderia cepacia
Infecções Relacionadas a Cateter/microbiologia
Serviço Hospitalar de Emergência
Endocardite Bacteriana/microbiologia
Enterococcus faecalis
Enterococcus faecium
Escherichia coli
Infecções por Escherichia coli/sangue
Infecções por Escherichia coli/microbiologia
Feminino
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Seres Humanos
Unidades de Terapia Intensiva
Infecções por Klebsiella/sangue
Infecções por Klebsiella/microbiologia
Klebsiella pneumoniae
Masculino
Meia-Idade
Pneumonia Bacteriana/microbiologia
Estudos Retrospectivos
Infecções Estafilocócicas/sangue
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus haemolyticus
Staphylococcus hominis
Infecções Urinárias/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 9007-12-9 (Calcitonin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


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[PMID]:27934830
[Au] Autor:Singh S; Dhawan B; Kapil A; Kabra SK; Suri A; Sreenivas V; Das BK
[Ad] Endereço:Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
[Ti] Título:Coagulase-negative staphylococci causing blood stream infection at an Indian tertiary care hospital: Prevalence, antimicrobial resistance and molecular characterisation.
[So] Source:Indian J Med Microbiol;34(4):500-505, 2016 Oct-Dec.
[Is] ISSN:1998-3646
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Recent years have seen a rise of coagulase-negative staphylococci (CoNS) from common contaminants to agents of nosocomial blood stream infections (BSI's). Molecular typing and establishing a correlation with antibiotic resistance is essential particularly in countries like India where genotyping studies for drug-resistant CoNS are sparse. METHODS: A prospective study was done over 18 months, wherein 42,693 blood samples were received, and 59 patients with BSI due to CoNS were evaluated. The isolates recovered were identified by a biochemical test panel and matrix-assisted laser desorption ionization - time of flight mass spectrometry followed by antimicrobial susceptibility testing by Kirby-Baur disc diffusion method and E-test strips. Staphylococcal chromosomal cassette mec (SCCmec) element was characterised by multiplex polymerase chain reaction for all methicillin-resistant (MR) isolates. RESULTS: The majority of CoNS isolated were constituted by Staphylococcus haemolyticus (47.5%) followed by Staphylococcus epidermidis (33.9%), Staphylococcus hominis (11.86%), Staphylococcus cohnii (5.08%) and Staphylococcus warneri (1.69%). Among all isolates 57.6% were MR with statistically significant higher resistance versus methicillin sensitive-CoNS. This difference was significant for erythromycin (76% vs. 44%, P = 0.011), rifampicin (50% vs. 12%,P= 0.002) and amikacin (26.5% vs. 4%, P = 0.023), ciprofloxacin (64.7% vs. 20%, P = 0.001) and cotrimoxazole (55.9% vs. 20%, P = 0.006). SCCmec type I was predominant (61.8%, P = 0.028) and exhibited multidrug resistance (76.2%). Coexistence of SCCmec type I and III was seen in 8.82% MR isolates. CONCLUSION: CoNS exhibit high antimicrobial resistance thereby limiting treatment options. The presence of new variants of SCCmec type in hospital-acquired CoNS may predict the antibiotic resistance pattern. This is the first evaluation of the molecular epidemiology of CoNS causing BSI from India and can serve as a guide in the formulation of hospital infection control and treatment guidelines.
[Mh] Termos MeSH primário: Bacteriemia/epidemiologia
Coagulase/análise
Infecção Hospitalar/epidemiologia
Farmacorresistência Bacteriana
Tipagem Molecular
Infecções Estafilocócicas/epidemiologia
Staphylococcus/isolamento & purificação
[Mh] Termos MeSH secundário: Bacteriemia/microbiologia
Técnicas de Tipagem Bacteriana
Infecção Hospitalar/microbiologia
Genótipo
Seres Humanos
Índia/epidemiologia
Testes de Sensibilidade Microbiana
Prevalência
Estudos Prospectivos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Infecções Estafilocócicas/microbiologia
Staphylococcus/classificação
Staphylococcus/efeitos dos fármacos
Staphylococcus/genética
Staphylococcus epidermidis
Staphylococcus haemolyticus
Staphylococcus hominis
Centros de Atenção Terciária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coagulase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.4103/0255-0857.195374


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[PMID]:27930711
[Au] Autor:Cirkovic I; Bozic DD; Draganic V; Lozo J; Beric T; Kojic M; Arsic B; Garalejic E; Djukic S; Stankovic S
[Ad] Endereço:Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
[Ti] Título:Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates.
[So] Source:PLoS One;11(12):e0167995, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Coagulase negative staphylococci (CoNS) and Listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. The aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of CoNS and L. monocytogenes genital isolates. METHODS: The effects of licheniocin 50.2 from Bacillus licheniformis VPS50.2 and crude extract of bacteriocins produced by Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 (BGBU1-4 crude extract) were evaluated on biofilm formation and formed biofilms of eight CoNS (four S. epidermidis, two S. hominis, one S. lugdunensis and one S. haemolyticus) and 12 L. monocytogenes genital isolates. RESULTS: Licheniocin 50.2 and BGBU1-4 crude extract inhibited the growth of both CoNS and L. monocytogenes isolates, with MIC values in the range between 200-400 AU/ml for licheniocin 50.2 and 400-3200 AU/ml for BGBU1-4 crude extract. Subinhibitory concentrations (1/2 × and 1/4 × MIC) of licheniocin 50.2 inhibited biofilm formation by all CoNS isolates (p < 0.05, respectively), while BGBU1-4 crude extract inhibited biofilm formation by all L. monocytogenes isolates (p < 0.01 and p < 0.05, respectively). Both bacteriocins in concentrations of 100 AU/mL and 200 AU/mL reduced the amount of 24 h old CoNS and L. monocytogenes biofilms (p < 0.05, p < 0.01, p < 0.001). CONCLUSIONS: This study suggests that novel bacteriocins have potential to be used for genital application, to prevent biofilm formation and/or to eradicate formed biofilms, and consequently reduce genital and neonatal infections by CoNS and L. monocytogenes.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bacillus licheniformis/fisiologia
Bacteriocinas/farmacologia
Biofilmes/efeitos dos fármacos
Lactococcus lactis/fisiologia
Listeria monocytogenes/efeitos dos fármacos
Staphylococcus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Bacillus licheniformis/metabolismo
Biofilmes/crescimento & desenvolvimento
Seres Humanos
Lactococcus lactis/metabolismo
Listeria monocytogenes/crescimento & desenvolvimento
Testes de Sensibilidade Microbiana
Staphylococcus/crescimento & desenvolvimento
Staphylococcus epidermidis/efeitos dos fármacos
Staphylococcus epidermidis/crescimento & desenvolvimento
Staphylococcus haemolyticus/efeitos dos fármacos
Staphylococcus haemolyticus/crescimento & desenvolvimento
Staphylococcus hominis/efeitos dos fármacos
Staphylococcus hominis/crescimento & desenvolvimento
Staphylococcus lugdunensis/efeitos dos fármacos
Staphylococcus lugdunensis/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacteriocins); 0 (licheniocin 50.2, Lactococcus lactis)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161209
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0167995


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[PMID]:27873736
[Au] Autor:Machet P; Lanotte P; Giraudeau B; Leperlier M; Tavernier E; Maruani A
[Ad] Endereço:University François Rabelais Tours,, CHRU Tours, Department of Dermatology, Unit of Paediatric Dermatology.
[Ti] Título:Amber necklaces: reasons for use and awareness of risk associated with bacterial colonisation.
[So] Source:Eur J Dermatol;26(6):580-585, 2016 Dec 01.
[Is] ISSN:1952-4013
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Parents are increasingly placing amber necklaces on their infants or toddlers to prevent teething pain. The use of the necklaces can pose a risk of death by strangulation, however, there are no data on the potential infectious risk linked to bacterial colonisation associated with the necklaces. We aimed to analyse bacterial colonisation of amber necklaces worn by children during hospital consultations. This prospective observational study included all children wearing a teething necklace at consultation in the Paediatric Dermatology and the Paediatric Emergency Department of our hospital from April to December 2014. The study included 27 children (median age: 10.7 months; 70.4% female). The surface of necklaces underwent bacteriological analyses using three different agar cultures. Parents completed a brief questionnaire to provide reasons for using necklaces and express awareness of risks. One child had a history of impetigo. All necklaces were colonised by bacteria (median: four species per necklace [range: 1-9]); 32 different species were found, the most frequent being coagulase-negative staphylococci (Staphylococcus epidermidis in 88.9% of cases). In three cases, methicillin-sensitive Staphylococcus aureus was found. The most frequent reason for wearing a necklace was to prevent teething pain (n = 17; 63.0%); the necklace was judged effective (moderately/highly effective) in 74.1% of cases, however, 70.4% of parents considered the use of the necklace to be risky. Amber necklaces may be highly colonised by commensal germs of the skin, mainly coagulase-negative staphylococci. Although these bacteria are saprophytes, they may become pathogenic in particular conditions.
[Mh] Termos MeSH primário: Âmbar
Fômites/microbiologia
Conhecimentos, Atitudes e Prática em Saúde
Joias/microbiologia
Staphylococcus/isolamento & purificação
[Mh] Termos MeSH secundário: Âmbar/efeitos adversos
Contagem de Colônia Microbiana
Feminino
Seres Humanos
Lactente
Joias/efeitos adversos
Masculino
Dor/prevenção & controle
Estudos Prospectivos
Staphylococcus aureus/isolamento & purificação
Staphylococcus epidermidis/isolamento & purificação
Staphylococcus hominis/isolamento & purificação
Inquéritos e Questionários
Erupção Dentária
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Amber)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE
[do] DOI:10.1684/ejd.2016.2871


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[PMID]:27720189
[Au] Autor:Rougé A; Wintzer-Wehekind J; Demailly B; Abdellaoui M; Faurie B; Monségu J
[Ad] Endereço:Institut cardiovasculaire, groupe hospitalier mutualiste de Grenoble, 8, rue Dr.-Calmette, 38000 Grenoble, France. Electronic address: A.ROUGE@ghm-grenoble.fr.
[Ti] Título:[Purulent pericarditis in a patient with diabetes mellitus treated by percutaneous pericardiocentesis].
[Ti] Título:Péricardite purulente chez un patient diabétique traitée par drainage péricardique percutané..
[So] Source:Ann Cardiol Angeiol (Paris);65(5):370-372, 2016 Nov.
[Is] ISSN:1768-3181
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:Purulent pericarditis seldom occurs in Western countries, yet its mortality rate remains high between 20 and 35 % despite early treatment. We report the case of a 43-year-old patient admitted in the intensive cardiologic care unit with a pre-tamponade, requiring an immediate percutaneous pericardiocentesis allowing the drainage of a purulent effusion. Evolution with antibiotic therapy adapted according to the bacteriological findings was favorable and 3-months follow-up shows a near complete regression of the effusion. This case recalls us this rare diagnosis entity and illustrates the possibility of a mere percutaneous pericardial drainage with the condition of a strict medical surveillance.
[Mh] Termos MeSH primário: Complicações do Diabetes/terapia
Derrame Pericárdico/terapia
Pericardiocentese/métodos
Pericardite/terapia
Infecções Estafilocócicas/terapia
Staphylococcus hominis
Infecções Estreptocócicas/terapia
Streptococcus intermedius
[Mh] Termos MeSH secundário: Adulto
Antibacterianos/uso terapêutico
Tamponamento Cardíaco/terapia
Terapia Combinada
Drenagem
Seguimentos
Seres Humanos
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161011
[St] Status:MEDLINE


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[PMID]:27439935
[Au] Autor:Geçmen Ç; Geçmen G; Kahyaoglu M; Hatipoglu S; Aksüt M
[Ad] Endereço:Department of Cardiology, Kartal Kosuyolu Heart and Research Hospital, Istanbul, Turkey. koronerr@hotmail.com.
[Ti] Título:Case images: Catheter tip covered by mass in the right atrium: An unusual example of infective endocarditis.
[So] Source:Turk Kardiyol Dern Ars;44(5):446, 2016 Jul.
[Is] ISSN:1308-4488
[Cp] País de publicação:Turkey
[La] Idioma:eng
[Mh] Termos MeSH primário: Infecções Relacionadas a Cateter
Cateteres/microbiologia
Endocardite
Átrios do Coração
[Mh] Termos MeSH secundário: Adulto
Bacteriemia
Infecções Relacionadas a Cateter/diagnóstico por imagem
Infecções Relacionadas a Cateter/cirurgia
Endocardite/diagnóstico por imagem
Endocardite/cirurgia
Feminino
Átrios do Coração/diagnóstico por imagem
Átrios do Coração/cirurgia
Seres Humanos
Infecções Estafilocócicas
Staphylococcus hominis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160722
[St] Status:MEDLINE
[do] DOI:10.5543/tkda.2016.32966


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[PMID]:26838903
[Au] Autor:Krupa AN; Vimala R
[Ad] Endereço:Bioremediation Lab, School of Biosciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India.
[Ti] Título:Evaluation of tetraethoxysilane (TEOS) sol-gel coatings, modified with green synthesized zinc oxide nanoparticles for combating microfouling.
[So] Source:Mater Sci Eng C Mater Biol Appl;61:728-35, 2016 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Green synthesis of zinc oxide nanoparticles (ZnO-NPs) is gaining importance as an eco-friendly alternative to conventional methods due to its enormous applications. The present work reports the synthesis of ZnO-NPs using the endosperm of Cocos nucifera (coconut water) and the bio-molecules responsible for nanoparticle formation have been identified. The synthesized nanoparticles were characterized using UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Transmission Electron Microscopy (TEM) and Zeta potential measurement. The results obtained reveal that the synthesized nanoparticles are moderately stable with the size ranging from 20 to 80 nm. The bactericidal effect of the nanoparticles was proved by well diffusion assay and determination of minimum inhibitory concentration (MIC) against marine biofilm forming bacteria. Further the green synthesized ZnO-NPs were doped with TEOS sol-gels (TESGs) in order to assess their antimicrofouling capability. Different volumes of liquid sol-gels were coated on to 96-well microtitre plate and cured under various conditions. The optimum curing conditions were found to be temperature 60 °C, time 72 h and volume 200 µl. Antiadhesion test of the undoped (SG) and ZnO-NP doped TEOS sol-gel (ZNSG) coatings were evaluated using marine biofilm forming bacteria. ZNSG coatings exhibited highest biofilm inhibition (89.2%) represented by lowest OD value against Pseudomonasotitidis strain NV1.
[Mh] Termos MeSH primário: Géis/química
Nanopartículas Metálicas/química
Silanos/química
Óxido de Zinco/química
[Mh] Termos MeSH secundário: Biofilmes/efeitos dos fármacos
Enterobacter cloacae/efeitos dos fármacos
Enterobacter cloacae/fisiologia
Química Verde
Nanopartículas Metálicas/toxicidade
Testes de Sensibilidade Microbiana
Microscopia Eletrônica de Varredura
Tamanho da Partícula
Pseudomonas/efeitos dos fármacos
Pseudomonas/fisiologia
Espectroscopia de Infravermelho com Transformada de Fourier
Staphylococcus hominis/efeitos dos fármacos
Staphylococcus hominis/fisiologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Gels); 0 (Silanes); 42064KRE49 (tetraethoxysilane); SOI2LOH54Z (Zinc Oxide)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE


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[PMID]:26659392
[Au] Autor:Hassan Z; Mustafa S; Rahim RA; Isa NM
[Ad] Endereço:Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
[Ti] Título:Anti-breast cancer effects of live, heat-killed and cytoplasmic fractions of Enterococcus faecalis and Staphylococcus hominis isolated from human breast milk.
[So] Source:In Vitro Cell Dev Biol Anim;52(3):337-48, 2016 Mar.
[Is] ISSN:1543-706X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Development of tumour that is resistant to chemotherapeutics and synthetic drugs, coupled with their life-threatening side effects and the adverse effects of surgery and hormone therapies, led to increased research on probiotics' anticancer potentials. The current study investigated the potential of live, heat-killed cells (HKC) and the cytoplasmic fractions (CF) of Enterococcus faecalis and Staphylococcus hominis as anti-breast cancer agents. MCF-7 cell line was treated with 25, 50, 100 and 200 µg/mL each of live, HKC and CF of the bacteria; and cytotoxicity was evaluated for 24, 48 and 72 h using MTT assay. The morphological features of the treated cells were examined by fluorescence microscopy. The stage of cell cycle arrest and apoptosis were quantified by flow cytometry. The bacterial effect on non-malignant breast epithelial cell line, MCF-10A, was assessed using MTT assay for 24, 48 and 72 h. All the three forms of the bacteria caused a significant decrease in MCF-7 (up to 33.29%) cell proliferation in concentration- and time-dependent manner. Morphological features of apoptosis like cell death, cell shrinkage and membrane blebbing were observed. Flow cytometry analyses suggested that about 34.60% of treated MCF-7 was undergoing apoptosis. A strong anti-proliferative activity was efficiently induced through sub-G1 accumulation (up to 83.17%) in treated MCF-7 and decreased number in the G0/G1 phase (74.39%). MCF-10A cells treated with both bacteria showed no significant difference with the untreated (>90% viability). These bacteria can be used as good alternative nutraceutical with promising therapeutic indexes for breast cancer because of their non-cytotoxic effects to normal cells.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Apoptose/efeitos dos fármacos
Neoplasias da Mama/tratamento farmacológico
Citoplasma/metabolismo
Enterococcus faecalis/metabolismo
Leite Humano/microbiologia
Staphylococcus hominis/metabolismo
[Mh] Termos MeSH secundário: Neoplasias da Mama/patologia
Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Enterococcus faecalis/isolamento & purificação
Feminino
Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos
Seres Humanos
Células MCF-7
Probióticos
Staphylococcus hominis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171105
[Lr] Data última revisão:
171105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE
[do] DOI:10.1007/s11626-015-9978-8



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