Base de dados : MEDLINE
Pesquisa : B03.300.390.400.800.750.750 [Categoria DeCS]
Referências encontradas : 72 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 8 ir para página                    

  1 / 72 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28245081
[Au] Autor:Deutch CE
[Ad] Endereço:Department of Biology, Creighton University, Omaha, NE, USA.
[Ti] Título:Limited effectiveness of over-the-counter plant preparations used for the treatment of urinary tract infections as inhibitors of the urease activity from Staphylococcus saprophyticus.
[So] Source:J Appl Microbiol;122(5):1380-1388, 2017 May.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: Urease is a key virulence factor for the Gram-positive urinary tract pathogen Staphylococcus saprophyticus and a potential target for antimicrobial therapy. The enzyme from S. saprophyticus is unusual in that it does not contain cysteine at the active site. The aims of this study were to test 14 over-the-counter plant preparations as inhibitors of this urease and to determine whether they can prevent the increase in pH that normally occurs in bacterial cultures containing urea. METHODS AND RESULTS: Urease activity was measured colorimetrically by the formation of ammonium ions. The green tea and Uva-Ursi preparations reduced urease activity in a soluble extract of S. saprophyticus by more than 75%. Two herbal mixtures were weakly inhibitory and reduced activity by about 25%, but the other products had little or no effect. The green tea and Uva-Ursi extracts also inhibited urease activity in whole cells by more than 75%. One of the herbal products (WishGarden UTI) showed some inhibition of urease activity but the other (UTI Clear) did not. The green tea and Uva-Ursi preparations prevented the increase in pH that normally occurs when S. saprophyticus is grown in an artificial urine medium, but this was due primarily to bacterial death. The WishGarden UTI preparation could partially delay the pH increase while allowing some cells to remain viable. CONCLUSION: These results indicate that only a few of the commercially available over-the-counter plant preparations commonly used for the treatment of urinary tract infections (UTIs) can inhibit the urease activity from S. saprophyticus. SIGNIFICANCE AND IMPACT OF THE STUDY: While over-the-counter plant preparations may be considered an alternative to traditional antibiotics for the treatment of UTIs, they should be used with caution and a product should be matched to the properties of the virulence factors of the bacterial pathogen involved.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/antagonistas & inibidores
Preparações de Plantas/farmacologia
Plantas/química
Staphylococcus saprophyticus/enzimologia
Staphylococcus saprophyticus/isolamento & purificação
Urease/antagonistas & inibidores
Infecções Urinárias/microbiologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Proteínas de Bactérias/metabolismo
Seres Humanos
Cinética
Preparações de Plantas/química
Staphylococcus/efeitos dos fármacos
Staphylococcus saprophyticus/efeitos dos fármacos
Staphylococcus saprophyticus/genética
Urease/metabolismo
Infecções Urinárias/tratamento farmacológico
Fatores de Virulência/antagonistas & inibidores
Fatores de Virulência/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Plant Preparations); 0 (Virulence Factors); EC 3.5.1.5 (Urease)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13430


  2 / 72 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28130140
[Au] Autor:Retnoningrum DS; Arumsari S; Artarini A; Ismaya WT
[Ad] Endereço:Laboratory of Pharmaceutical Biotechnology, School of Pharmacy, Bandung Institute of Technology, Jalan Ganesa No. 10, Bandung, 40132, Indonesia. Electronic address: retnoningrum@indo.net.id.
[Ti] Título:Structure-activity relationship of a recombinant hybrid Manganese superoxide dismutase of Staphylococcus saprophyticus/S. equorum.
[So] Source:Int J Biol Macromol;98:222-227, 2017 May.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Recombinant hybrid Manganese superoxide dismutase from Staphyloccus saphropyticus/S. equorum (rMnSODSeq) exhibits stability at high temperatures. The enzyme occurs as a dimer that dissociates around 52°C prior to unfolding of the monomer around 64°C, demonstrating contribution of the dimeric form to stability. Here, structure - activity relationship of rMnSODSeq was evaluated on the basis of its activity and stability in the presence of inhibitors, NaCl, denaturants, detergents, reducing agents, and at different pH values. The activity was evaluated at both 37°C and 52°C, which the latter is the temperature for dissociation of the dimer. Dimer to monomer transition coincided with significant decrease in residual activity at 52°C. However, the activity assay results at 52°C and 37°C suggest spontaneous re-association of the monomer into dimer. Intriguingly, various new species with melting temperature (T ) values other than those of the dimer or monomer were observed. These species displayed medium to comparable level of residual activities to the native at 37°C. This report suggests that dimer to monomer transition may be not the only explanation for activity loss or decrease.
[Mh] Termos MeSH primário: Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Staphylococcus saprophyticus/enzimologia
Superóxido Dismutase/química
Superóxido Dismutase/metabolismo
[Mh] Termos MeSH secundário: Detergentes/farmacologia
Inibidores Enzimáticos/farmacologia
Concentração de Íons de Hidrogênio
Desnaturação Proteica/efeitos dos fármacos
Substâncias Redutoras/farmacologia
Cloreto de Sódio/farmacologia
Relação Estrutura-Atividade
Superóxido Dismutase/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Detergents); 0 (Enzyme Inhibitors); 0 (Recombinant Proteins); 0 (Reducing Agents); 451W47IQ8X (Sodium Chloride); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170426
[Lr] Data última revisão:
170426
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE


  3 / 72 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:27889420
[Au] Autor:Ferreira AM; Martins KB; Silva VR; Mondelli AL; Cunha ML
[Ad] Endereço:Universidade Estadual Paulista (UNESP), Botucatu Biosciences Institute, Department of Microbiology and Immunology, Botucatu, SP, Brazil; Universidade Estadual Paulista (UNESP), Botucatu School of Medicine University Hospital, Department of Tropical Diseases, Botucatu, SP, Brazil.
[Ti] Título:Correlation of phenotypic tests with the presence of the blaZ gene for detection of beta-lactamase.
[So] Source:Braz J Microbiol;48(1):159-166, 2017 Jan - Mar.
[Is] ISSN:1678-4405
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Staphylococcus aureus and Staphylococcus saprophyticus are the most common and most important staphylococcal species associated with urinary tract infections. The objective of the present study was to compare and to evaluate the accuracy of four phenotypic methods for the detection of beta-lactamase production in Staphylococcus spp. Seventy-three strains produced a halo with a diameter ≤28mm (penicillin resistant) and all of them were positive for the blaZ gene. Among the 28 susceptible strain (halo ≥29mm), 23 carried the blaZ gene and five did not. The zone edge test was the most sensitive (90.3%), followed by MIC determination (85.5%), but the specificity of the former was low (40.0%). The nitrocefin test was the least sensitive (28.9%). However, the nitrocefin test together with the disk diffusion method showed the highest specificity (100%). The present results demonstrated that the zone edge test was the most sensitive phenotypic test for detection of beta-lactamase, although it is still not an ideal test to detect this type of resistance since its specificity was low. However, the inhibition halo diameter of the penicillin disk can be used together with the zone edge test since the same disk is employed in the two tests. Combined analysis of the two tests shows a sensitivity of 90.3% and specificity of 100%, proving better sensitivity, especially for S. saprophyticus. This is a low-cost test of easy application and interpretation that can be used in small and medium-sized laboratories where susceptibility testing is usually performed by the disk diffusion method.
[Mh] Termos MeSH primário: Testes de Sensibilidade Microbiana
Resistência beta-Lactâmica
beta-Lactamases/genética
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Genótipo
Resistência às Penicilinas
Sensibilidade e Especificidade
Infecções Estafilocócicas/microbiologia
Staphylococcus saprophyticus/efeitos dos fármacos
Staphylococcus saprophyticus/genética
Staphylococcus saprophyticus/metabolismo
Infecções Urinárias/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161128
[St] Status:MEDLINE


  4 / 72 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27733519
[Au] Autor:Sadahira T; Wada K; Araki M; Ishii A; Takamoto A; Kobayashi Y; Watanabe M; Watanabe T; Nasu Y; Kumon H; Okayama Urological Research Group (OURG)
[Ad] Endereço:Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
[Ti] Título:Efficacy and safety of 3 day versus 7 day cefditoren pivoxil regimens for acute uncomplicated cystitis: multicentre, randomized, open-label trial.
[So] Source:J Antimicrob Chemother;72(2):529-534, 2017 Feb.
[Is] ISSN:1460-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Fluoroquinolone-non-susceptible Escherichia coli isolated from patients with acute uncomplicated cystitis are a matter of increasing concern. Cefditoren pivoxil is an oral, ß-lactamase-stable, extended-spectrum cephalosporin that is effective against fluoroquinolone-non-susceptible bacteria. OBJECTIVES: To evaluate the clinical and microbiological efficacies of cefditoren pivoxil against acute uncomplicated cystitis and to determine the optimal duration of cefditoren pivoxil treatment. METHODS: We compared 3 and 7 day regimens of cefditoren pivoxil in a multicentre, randomized, open-label study. RESULTS: A total of 104 female patients with acute uncomplicated cystitis were enrolled and randomized into 3 day (n = 51) or 7 day (n = 53) treatment groups. At first visit, 94 bacterial strains were isolated from the 104 participants of which 81.7% (85/104) were E. coli. Clinical and microbiological efficacies were evaluated 5-9 days following administration of the final dose of cefditoren pivoxil. The clinical efficacies of the 3 and 7 day groups were 90.9% (40/44) and 93.2% (41/44), respectively (P = 1.000). The microbiological efficacies of the 3 and 7 day groups were 82.5% (33/40) and 90.2% (37/41), respectively (P = 0.349). There were no adverse events due to cefditoren pivoxil treatment, with the exception of a mild allergic reaction in one patient, after which the cefditoren pivoxil was exchanged for another antimicrobial. CONCLUSIONS: Cefditoren pivoxil is safe and effective for uncomplicated cystitis, with no significant differences in clinical and microbiological efficacies between 3 and 7 day regimens.
[Mh] Termos MeSH primário: Antibacterianos/administração & dosagem
Antibacterianos/uso terapêutico
Cefalosporinas/administração & dosagem
Cefalosporinas/uso terapêutico
Cistite/tratamento farmacológico
Cistite/microbiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Antibacterianos/efeitos adversos
Cefalosporinas/efeitos adversos
Citrobacter koseri/efeitos dos fármacos
Citrobacter koseri/isolamento & purificação
Farmacorresistência Bacteriana
Enterococcus faecalis/efeitos dos fármacos
Enterococcus faecalis/isolamento & purificação
Escherichia coli/efeitos dos fármacos
Escherichia coli/isolamento & purificação
Infecções por Escherichia coli/tratamento farmacológico
Infecções por Escherichia coli/microbiologia
Feminino
Fluoroquinolonas/farmacologia
Seres Humanos
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/isolamento & purificação
Testes de Sensibilidade Microbiana
Meia-Idade
Staphylococcus saprophyticus/efeitos dos fármacos
Staphylococcus saprophyticus/isolamento & purificação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephalosporins); 0 (Fluoroquinolones); 78THA212DH (cefditoren pivoxil)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161014
[St] Status:MEDLINE
[do] DOI:10.1093/jac/dkw424


  5 / 72 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27629897
[Au] Autor:Bonjean M; Hodille E; Dumitrescu O; Dupieux C; Nkoud Mongo C; Allam C; Beghin M; Paris M; Borrel O; Chardon H; Laurent F; Rasigade JP; Lina G
[Ad] Endereço:IAI, Hospices Civils de Lyon, Lyon, France.
[Ti] Título:Disk Diffusion Testing for Detection of Methicillin-Resistant Staphylococci: Does Moxalactam Improve upon Cefoxitin?
[So] Source:J Clin Microbiol;54(12):2905-2909, 2016 Dec.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Disk diffusion testing is widely used to detect methicillin resistance in staphylococci, and cefoxitin is currently considered the best marker for mecA-mediated methicillin resistance. In low-inoculum diffusion testing (colony suspension at 10 CFU/ml), the addition of moxalactam in combination with cefoxitin has been reported to improve on cefoxitin alone for the detection of methicillin-heteroresistant staphylococci. However, moxalactam is absent from EUCAST and CLSI guidelines, which use high-inoculum diffusion testing (colony suspension at 10 CFU/ml), calling into question the potential interest of including moxalactam in their recommendations. The inhibition zone diameters of cefoxitin and moxalactam, alone and in combination, were evaluated for concordance with mecA and mecC positivity in a large collection of clinical Staphylococcus isolates (611 Staphylococcus aureus, Staphylococcus lugdunensis, and Staphylococcus saprophyticus isolates and 307 coagulase-negative staphylococci other than S. lugdunensis and S. saprophyticus isolates, of which 22% and 53% were mecA-positive, respectively) and in 25 mecC-positive S. aureus isolates using high-inoculum diffusion testing. Receiver operating characteristic, sensitivity, and specificity analyses indicated that the detection of mecA- and mecC-positive and negative isolates did not improve with moxalactam, either alone or in combination with cefoxitin, compared to cefoxitin alone. These findings were similar in both the S. aureus/S. lugdunensis/S. saprophyticus group and in the coagulase-negative staphylococci group. Our results do not support the use of moxalactam as an additional marker of methicillin resistance when testing with high-inoculum disk diffusion.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Cefoxitina/farmacologia
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/métodos
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Moxalactam/farmacologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Seres Humanos
Staphylococcus aureus Resistente à Meticilina/genética
Proteínas de Ligação às Penicilinas/genética
Staphylococcus lugdunensis/efeitos dos fármacos
Staphylococcus lugdunensis/genética
Staphylococcus lugdunensis/isolamento & purificação
Staphylococcus saprophyticus/efeitos dos fármacos
Staphylococcus saprophyticus/genética
Staphylococcus saprophyticus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Penicillin-Binding Proteins); 0 (mecA protein, Staphylococcus aureus); 6OEV9DX57Y (Cefoxitin); VUF6C936Z3 (Moxalactam)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE


  6 / 72 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27601383
[Au] Autor:Paiva-Santos W; Barros EM; Sousa VS; Laport MS; Giambiagi-deMarval M
[Ad] Endereço:Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde (CCS), Av. Carlos Chagas Filho, 373, Bloco I, Cidade Universitária, 21941-920Rio de Janeiro, RJ, Brazil.
[Ti] Título:Identification of coagulase-negative Staphylococcus saprophyticus by polymerase chain reaction based on the heat-shock repressor encoding hrcA gene.
[So] Source:Diagn Microbiol Infect Dis;86(3):253-256, 2016 Nov.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Staphylococcus saprophyticus is an uropathogen belonging to the human microbiota and is responsible for community-acquired infections of the urinary tract. Identification of Staphylococcus species by biochemical tests is laborious and costly when compared to routine laboratory tests. Because of their high sensitivity and specificity, molecular methods are better suited for accurate identification of Staphylococcusspp. Therefore, the goal of this work was to standardize a polymerase chain reaction (PCR) protocol using species-specific primers, based on the heat-shock repressor coding hrcA gene, for the identification of S.saprophyticus. A total of 142 S. saprophyticus strains were obtained from different sources, including clinical, environmental, and foodborne strains. We also included 98 strains of Staphylococcus spp. to further validate the proposed method. Reliable results for the detection of S. saprophyticus isolates were obtained for 100% of the strains evaluated. The results were in accordance with matrix-assisted laser desorption ionization-time of flight mass spectrometry identification, thus highlighting the applicability of species-specific PCR for the molecular identification of S. saprophyticus.
[Mh] Termos MeSH primário: Técnicas de Diagnóstico Molecular/métodos
Reação em Cadeia da Polimerase/métodos
Proteínas Repressoras/genética
Staphylococcus saprophyticus/classificação
Staphylococcus saprophyticus/isolamento & purificação
[Mh] Termos MeSH secundário: Microbiologia Ambiental
Feminino
Microbiologia de Alimentos
Seres Humanos
Gravidez
Complicações Infecciosas na Gravidez/microbiologia
Sensibilidade e Especificidade
Infecções Estafilocócicas/microbiologia
Staphylococcus saprophyticus/genética
Infecções Urinárias/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Repressor Proteins)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170208
[Lr] Data última revisão:
170208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE


  7 / 72 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27566110
[Au] Autor:Phitaktim S; Chomnawang M; Sirichaiwetchakoon K; Dunkhunthod B; Hobbs G; Eumkeb G
[Ad] Endereço:School of Pharmacology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
[Ti] Título:Synergism and the mechanism of action of the combination of α-mangostin isolated from Garcinia mangostana L. and oxacillin against an oxacillin-resistant Staphylococcus saprophyticus.
[So] Source:BMC Microbiol;16(1):195, 2016 Aug 26.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Globally, staphylococci have developed resistance to many antibiotics. New approaches to chemotherapy are needed and one such approach could be to use plant derived actives with conventional antibiotics in a synergestic way. The purpose of this study was to isolate α-mangostin from the mangosteen (Garcinia mangostana L.; GML) and investigate antibacterial activity and mechanisms of action when used singly and when combined with oxacillin against oxacillin-resistant Staphylococcus saprophyticus (ORSS) strains. The isolated α-mangostin was confirmed by HPLC chromatogram and NMR spectroscopy. The minimum inhibitory concentration (MIC), checkerboard and killing curve were determined. The modes of action of these compounds were also investigated by enzyme assay, transmission electron microscopy (TEM), confocal microscopic images, and cytoplasmic membrane (CM) permeabilization studies. RESULTS: The MICs of isolated α-mangostin and oxacillin against these strains were 8 and 128 µg/ml, respectively. Checkerboard assays showed the synergistic activity of isolated α-mangostin (2 µg/ml) plus oxacillin (16 µg/ml) at a fractional inhibitory concentration index (FICI) of 0.37. The kill curve assay confirmed that the viability of oxacillin-resistant Staphylococcus saprophyticus DMST 27055 (ORSS-27055) was dramatically reduced after exposure to isolated α-mangostin (2 µg/ml) plus oxacillin (16 µg/ml). Enzyme assays demonstrated that isolated α-mangostin had an inhibitory activity against ß-lactamase in a dose-dependent manner. TEM results clearly showed that these ORSS-27055 cells treated with this combination caused peptidoglycan and cytoplasmic membrane damage, irregular cell shapes and average cell areas were significantly larger than the control. Clearly, confocal microscopic images confirmed that this combination caused considerable peptidoglycan damage and DNA leakage. In addition, the CM permeability of ORSS-27055 was also increased by this combination of actives. CONCLUSIONS: These findings provide evidence that isolated α-mangostin alone has not only some activity but also shows the synergistic activity with oxacillin against ORSS-27055. The chromone and isoprenyl structures could play a significant role in its action. This synergistic activity may involve three mechanisms of action. Firstly, potential effects of cytoplasmic membrane disruption and increases permeability. Secondly, inhibit ß-lactamase activity. Finally, also damage to the peptidoglycan structure. We proposes the potential to develop a novel adjunct phytopharmaceutical to oxacillin for the treatment of ORSS. Future studies require clinical trials to establish if the synergy reported can be translated to animals and humans.
[Mh] Termos MeSH primário: Garcinia mangostana/química
Oxacilina/farmacologia
Staphylococcus saprophyticus/efeitos dos fármacos
Xantonas/farmacologia
[Mh] Termos MeSH secundário: Células 3T3-L1
Animais
Antibacterianos/farmacologia
Membrana Celular/efeitos dos fármacos
Membrana Celular/metabolismo
Permeabilidade da Membrana Celular/efeitos dos fármacos
DNA Bacteriano/efeitos dos fármacos
Farmacorresistência Bacteriana
Sinergismo Farmacológico
Ensaios Enzimáticos
Camundongos
Testes de Sensibilidade Microbiana
Viabilidade Microbiana/efeitos dos fármacos
Staphylococcus saprophyticus/citologia
Xantonas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Xanthones); U6RIV93RU1 (mangostin); UH95VD7V76 (Oxacillin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-016-0814-4


  8 / 72 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27252156
[Au] Autor:Marlinghaus L; Huß M; Korte-Berwanger M; Sakinc-Güler T; Gatermann SG
[Ad] Endereço:Department of Medical Microbiology, Institute for Hygiene and Microbiologie, Ruhr-University Bochum, Universitätsstraße 150, 44892 Bochum, Germany Lennart.Marlinghaus@rub.de.
[Ti] Título:D-serine transporter in Staphylococcus saprophyticus identified.
[So] Source:FEMS Microbiol Lett;363(14), 2016 Jul.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Among staphylococci Staphylococcus saprophyticus is the only species that is typically uropathogenic and an important cause of urinary tract infections in young women. The amino acid D-serine occurs in relatively high concentrations in human urine and has a bacteriostatic or toxic effect on many bacteria. In uropathogenic Escherichia coli and S. saprophyticus, the amino acid regulates the expression of virulence factors and can be used as a nutrient. The ability of uropathogens to respond to or to metabolize D-serine has been suggested as a factor that enables colonization of the urinary tract. Until now nothing is known about D-serine transport in S. saprophyticus We generated mutants of putative transporter genes in S. saprophyticus 7108 that show homology to the D-serine transporter cycA of E. coli and tested them in a D-serine depletion assay to analyze the D-serine uptake rate of the cells. The mutant of SPP1070 showed a strong decrease in D-serine uptake. Therefore, SSP1070 was identified as a major D-serine transporter in S. saprophyticus 7108 and was named D-serine transporter A (DstA). D-serine caused a prolonged lag phase of S. saprophyticus in a chemically defined medium. This negative effect was dependent on the presence of DstA.
[Mh] Termos MeSH primário: Sistemas de Transporte de Aminoácidos/genética
Sistemas de Transporte de Aminoácidos/metabolismo
Serina/metabolismo
Staphylococcus saprophyticus/genética
Staphylococcus saprophyticus/metabolismo
[Mh] Termos MeSH secundário: Alelos
Expressão Gênica
Mutação
Staphylococcus saprophyticus/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acid Transport Systems); 452VLY9402 (Serine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160603
[St] Status:MEDLINE


  9 / 72 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27133307
[Au] Autor:Dziri R; Klibi N; Lozano C; Ben Said L; Bellaaj R; Tenorio C; Boudabous A; Ben Slama K; Torres C
[Ad] Endereço:Laboratoire des Microorganismes et Biomolécules actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisie.
[Ti] Título:High prevalence of Staphylococcus haemolyticus and Staphylococcus saprophyticus in environmental samples of a Tunisian hospital.
[So] Source:Diagn Microbiol Infect Dis;85(2):136-40, 2016 Jun.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was to evaluate the rate of detection of coagulase negative staphylococci (CoNS) in environmental samples of 17 services in a Tunisian hospital, determining the antimicrobial resistance phenotypes and genotypes of recovered isolates. To our knowledge, this is the first study that determines the prevalence of CoNS with correlation of antibiotic resistance in the hospital environment in Tunisia. CoNS were obtained from 83 of the 200 tested samples (41.5%). Staphylococcus haemolyticus was the most prevalent species (45.8%), followed by S. saprophyticus (36.1%). The remaining CoNS species detected were S. epidermidis, S. cohnii, S. warneri, S. sciuri, S. simulans, S. pasteuri, S. arlettae, and S. xilosus. Methicillin-resistant CoNS were detected in 20 of the 200 tested samples (10%), and the mecA gene was demonstrated in 18 S. haemolyticus, one S. epidermidis and one S. saprophyticus isolates. Methicillin susceptible isolates were detected in 63 samples (31.5%). Antimicrobial resistance genes detected were as follows (number of isolates): erythromycin [msr(A) (n = 32); erm(C) (n = 8)], tetracycline [tet(K) and/or tet(M) (n = 21)], gentamicin [aac(6')-Ie-aph(2″)-Ia (n = 16)], kanamycin [(aph(3')-IIIa (n = 19)], tobramycin [ant(4')-Ia (n = 14)], and streptomycin [ant(6')-Ia (n = 3)]. The high frequency of detection of multi-drug-resistant CoNS in the hospital environment, especially S. haemolyticus and S. saprophyticus, is of relevance and could be due to cross-transmission between patients, staff, and environment.
[Mh] Termos MeSH primário: Microbiologia Ambiental
Staphylococcus haemolyticus/isolamento & purificação
Staphylococcus saprophyticus/isolamento & purificação
[Mh] Termos MeSH secundário: Farmacorresistência Bacteriana
Genes Bacterianos
Genótipo
Hospitais
Seres Humanos
Prevalência
Staphylococcus haemolyticus/classificação
Staphylococcus haemolyticus/efeitos dos fármacos
Staphylococcus haemolyticus/genética
Staphylococcus saprophyticus/classificação
Staphylococcus saprophyticus/efeitos dos fármacos
Staphylococcus saprophyticus/genética
Tunísia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170208
[Lr] Data última revisão:
170208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160503
[St] Status:MEDLINE


  10 / 72 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27020869
[Au] Autor:Adeghate J; Juhász E; Pongrácz J; Rimanóczy É; Kristóf K
[Ad] Endereço:Department of Laboratory Medicine, Semmelweis University , Budapest , Hungary.
[Ti] Título:Does Staphylococcus Saprophyticus Cause Acute Cystitis only in Young Females, or is there more to the Story? A One-Year Comprehensive Study Done in Budapest, Hungary.
[So] Source:Acta Microbiol Immunol Hung;63(1):57-67, 2016 Mar.
[Is] ISSN:1217-8950
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:Staphylococcus saprophyticus is a well-known urinary pathogen in acute cystitis in young females. We completed a retrospective overview of the distribution of urinary tract infections (UTIs) occurring in 2014, at Semmelweis University hospitals and at Heim Pál Children's Hospital. Six age-groups (ages 0-100) were examined, with the frequency of S. saprophyticus in females being: 0.1% (0-4), 0.7%, (5-15), 7.4% (16-24), 1.2% (25-39), 0.4% (40-59) and 0.1% (60-100), and S. saprophyticus being the 3(rd) most common pathogen in females aged 16-24. In males, S. saprophyticus was only isolated from those aged 5-15. Seasonal distribution of UTIs caused by S. saprophyticus showed that most infections occurred during the months of January, June, August and November. Antibiotic-resistance rates of amoxicillin, clindamycin, doxycycline, erythromycin, gentamicin and sulfamethoxazole- trimethoprim varied as follows: 0.9%, 32.7%, 19.6%, 34.6%, 0.9% and 0.9%, respectively. Thirty randomly selected samples were analysed by pulsed-field gelelectrophoresis, and 28 different genotypes were identified. S. saprophyticus is involved in the pathogenesis of acute cystitis not only in young females, but also in other age-groups, and in young males as well. We did not find any significant seasonal occurrence in S. saprophyticus-caused UTIs. The infective strains were genetically diverse. Antibiotic-resistance does not pose any issue as of yet.
[Mh] Termos MeSH primário: Cistite/microbiologia
Infecções Estafilocócicas/microbiologia
Staphylococcus saprophyticus/fisiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Criança
Pré-Escolar
Cistite/epidemiologia
Feminino
Seres Humanos
Hungria/epidemiologia
Lactente
Masculino
Meia-Idade
Estudos Retrospectivos
Fatores Sexuais
Infecções Estafilocócicas/epidemiologia
Staphylococcus saprophyticus/isolamento & purificação
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160329
[Lr] Data última revisão:
160329
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160330
[St] Status:MEDLINE
[do] DOI:10.1556/030.63.2016.1.4



página 1 de 8 ir para página                    
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde