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[PMID]: 28951607 [Au] Autor: Sahu AK; Quadri SR; Agasar D; Ruwaili JA; Jun-Li W; Dastager SG [Ad] Endereço: Academy of Scientific and Innovative Research (AcSIR), and NCIM Resource Center, CSIR-National Chemical Laboratory, NCIM Resource Center, Pune, India. [Ti] Título: Allostreptomyces indica sp. nov., isolated from India. [So] Source: J Antibiot (Tokyo);70(10):1000-1003, 2017 Oct. [Is] ISSN: 0021-8820 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: A novel actinobacterium, designated strain YIM 75704 , was isolated from a limestone quarry located at Gulbarga, Karnataka, India. The novel strain has showed typical morphological and chemotaxonomic characteristics of the family Streptomycetaceae. Comparison of 16S rRNA gene sequences indicated that this strain represents a novel member of the family Streptomycetaceae and exhibited 99.0% 16S rRNA gene sequence similarities with the type species of the recently described novel genus Allostreptomyces, that is, Allostreptomyces psammosilenae, whereas other species of Streptomyces were below 95% sequence similarity. The cell hydrolysates contained the LL-isomer of diaminopimelic acid and the predominant quinones were MK-9 (H , H and H ). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylinositolmannosides and three unknown phospholipids. The DNA G+C content was 75.0 mol%. A polyphasic study of the strain with morphological, phenotypic, phylogenetic and with DNA-DNA hybridization evidence with related members showed that this strain represents novel species of Allostreptomyces for which the name Allostreptomyces indica sp. nov., is proposed. The type strain is YIM 75704 (= DSM 41985 =CCTCC AA 209051 = NCIM 5485 ). [Mh] Termos MeSH primário: Microbiologia Ambiental Streptomycetaceae/classificação Streptomycetaceae/isolamento & purificação [Mh] Termos MeSH secundário: Composição de Bases Parede Celular/química Análise por Conglomerados Citosol/química DNA Bacteriano/química DNA Bacteriano/genética DNA Ribossômico/química DNA Ribossômico/genética Ácido Diaminopimélico/análise Índia Hibridização de Ácido Nucleico Fosfolipídeos/análise Filogenia Quinonas/análise RNA Ribossômico 16S/genética Análise de Sequência de DNA Streptomycetaceae/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Phospholipids); 0 (Quinones); 0 (RNA, Ribosomal, 16S); 583-93-7 (Diaminopimelic Acid) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171010 [Lr] Data última revisão: 171010 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170928 [St] Status: MEDLINE [do] DOI: 10.1038/ja.2017.82

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[PMID]: 28196975 [Au] Autor: Momose I; Watanabe T [Ad] Endereço: Institute of Microbial Chemistry (BIKAKEN), Shizuoka, Japan. [Ti] Título: Tyropeptins, proteasome inhibitors produced by Kitasatospora sp. MK993-dF2. [So] Source: J Antibiot (Tokyo);70(5):542-550, 2017 May. [Is] ISSN: 0021-8820 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: Tyropeptins are new proteasome inhibitors isolated from the culture broth of Kitasatospora sp. MK993-dF2. Tyropeptins permeate cell membranes, inhibit intracellular proteasomes and reduce the degradation of ubiquitinated proteins in mammalian cells. We performed structure-based drug design and structure-activity relationship studies on tyropeptin derivatives to obtain valuable information of derivatives. Among the synthesized tyropeptin derivatives, some boronic acid derivatives exhibited potent antitumor effects against human multiple myeloma. In this review, we summarize the discovery of tyropeptins and the development of tyropeptin derivatives. [Mh] Termos MeSH primário: Dipeptídeos/isolamento & purificação Desenho de Drogas Streptomycetaceae/metabolismo [Mh] Termos MeSH secundário: Animais Antineoplásicos/química Antineoplásicos/isolamento & purificação Antineoplásicos/farmacologia Dipeptídeos/química Dipeptídeos/farmacologia Seres Humanos Complexo de Endopeptidases do Proteassoma Inibidores de Proteassoma/química Inibidores de Proteassoma/isolamento & purificação Inibidores de Proteassoma/farmacologia Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Antineoplastic Agents); 0 (Dipeptides); 0 (Proteasome Inhibitors); 0 (tyropeptin A); 0 (tyropeptin B); EC 3.4.25.1 (Proteasome Endopeptidase Complex) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170620 [Lr] Data última revisão: 170620 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170216 [St] Status: MEDLINE [do] DOI: 10.1038/ja.2017.9

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[PMID]: 28196972 [Au] Autor: Takahashi Y [Ad] Endereço: Kitasato Institute for Life Sciences, Kitasato University, Minato-ku, Tokyo, Japan. [Ti] Título: Genus Kitasatospora, taxonomic features and diversity of secondary metabolites. [So] Source: J Antibiot (Tokyo);70(5):506-513, 2017 May. [Is] ISSN: 0021-8820 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: The genus Kitasatospora was proposed in 1982. Although Kitasatospora strains resemble Streptomyces strains in morphology, they are clearly different in cell-wall composition, as they contain both LL- and meso-diaminopimelic acid. Aerial and submerged spores contain LL-, while vegetative and submerged mycelia contain mainly meso- in their cell walls. Currently, 23 species have been validly proposed. Members of the genus Kitasatospora form a tight cluster and represent a legitimate genus distinct from Streptomyces on the basis of phylogenetic analysis of 16S rRNA gene sequences. A variety of biologically active compounds have been found from Kitasatospora strains and structures of these compounds are extremely diverse. Genome sequences of 15 strains published so far are about 7-9 Mb in size and contain many genes governing secondary metabolites. [Mh] Termos MeSH primário: RNA Ribossômico 16S/genética Metabolismo Secundário/genética Streptomycetaceae/genética [Mh] Termos MeSH secundário: Parede Celular/química Genoma Bacteriano Filogenia Análise de Sequência de RNA Especificidade da Espécie Streptomyces/classificação Streptomyces/genética Streptomycetaceae/classificação Streptomycetaceae/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (RNA, Ribosomal, 16S) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170620 [Lr] Data última revisão: 170620 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170216 [St] Status: MEDLINE [do] DOI: 10.1038/ja.2017.8

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[PMID]: 27902296 [Au] Autor: Huang MJ; Rao MP; Salam N; Xiao M; Huang HQ; Li WJ [Ad] Endereço: 2​Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China 1​College of Landscape Architecture, Southwest Forestry University, Kunming 650224, PR China. [Ti] Título: Allostreptomyces psammosilenae gen. nov., sp. nov., an endophytic actinobacterium isolated from the roots of Psammosilene tunicoides and emended description of the family Streptomycetaceae [Waksman and Henrici (1943)AL] emend. Rainey et al. 1997, emend. Kim et al. 2003, emend. Zhi et al. 2009. [So] Source: Int J Syst Evol Microbiol;67(2):288-293, 2017 Feb. [Is] ISSN: 1466-5034 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A Gram-stain-positive actinobacterium, designated strain YIM DR4008T, was isolated from the root sample of Psammosilene tunicoides collected from Lijiang, Yunnan, China. Strain YIM DR4008T could grow at temperatures ranging from 10 to 50 °C (optimum 28-30 °C), at pH 5.0-11.0 (optimum pH 7.0) and in the presence of up to 4 % (w/v) NaCl. Sequence analysis of the 16S ribosomal RNA gene revealed that strain YIM DR4008T shared highest similarity (95.0 %) with Streptomyces griseoplanus NBRC 12779T and <95 % similarity with other known members of the genera Streptomyces, Kitasatospora and Streptacidiphilus. The diagnostic cell-wall diamino acid of strain YIM DR4008T was found to be ll-diaminopimelic acid. The whole-cell hydrolysates contained a major amount of galactose and mannose along with a small proportion of fucose, glucose, rhamnose and ribose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylinositol mannosides and three unidentified phospholipids. The respiratory menaquinones were MK-9(H6) and MK-9(H8), while the major cellular fatty acids (>10 %) were anteiso-C15 : 0, C16 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C17 : 0. The genomic DNA G+C content was determined to be 75.3 mol%. Based on the phenotypic, chemotaxonomic and molecular characteristics, strain YIM DR4008T is proposed to be recognized as a novel species of a new genus in the family Streptomycetaceae, with the name Allostreptomyces psammosilenae gen. nov., sp. nov. The type strain of the type species is YIM DR4008T (=DSM 42178T=CGMCC 4.7247T). An emended description of the family Streptomycetaceae is also provided. [Mh] Termos MeSH primário: Caryophyllaceae/microbiologia Filogenia Raízes de Plantas/microbiologia Streptomycetaceae/classificação [Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana Composição de Bases China DNA Bacteriano/genética Ácido Diaminopimélico/química Ácidos Graxos/química Fosfolipídeos/química RNA Ribossômico 16S/genética Análise de Sequência de DNA Streptomycetaceae/genética Streptomycetaceae/isolamento & purificação Vitamina K 2/análogos & derivados Vitamina K 2/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-39-7 (menaquinone 9); 583-93-7 (Diaminopimelic Acid) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170818 [Lr] Data última revisão: 170818 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161201 [St] Status: MEDLINE [do] DOI: 10.1099/ijsem.0.001617

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[PMID]: 27349134 [Au] Autor: Grossmann I; Döring C; Jekle M; Becker T; Koehler P [Ad] Endereço: Deutsche Forschungsanstalt für Lebensmittelchemie, Leibniz Institut , Lise-Meitner-Straße 34, 85354 Freising, Germany. [Ti] Título: Compositional Changes and Baking Performance of Rye Dough As Affected by Microbial Transglutaminase and Xylanase. [So] Source: J Agric Food Chem;64(28):5751-8, 2016 Jul 20. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Doughs supplemented with endoxylanase (XYL) and varying amounts of microbial transglutaminase (TG) were analyzed by sequential protein extraction, quantitation of protein fractions and protein types, and determination of water-extractable arabinoxylans. With increasing TG activity, the concentration of prolamins and glutelins decreased and increased, respectively, and the prolamin-to-glutelin ratio strongly declined. The overall amount of extractable protein decreased with increasing TG level showing that cross-linking by TG provided high-molecular-weight protein aggregates. The decrease of the high-molecular-weight arabinoxylan fraction and the concurrent increase of the medium-molecular-weight fraction confirmed the degradation of arabinoxylans by XYL. However, XYL addition did not lead to significant improved cross-linking of rye proteins by TG. Volume and crumb hardness measurements of bread showed increased protein connectivity induced by XYL and TG. Significant positive effects on the final bread quality were especially obtained by XYL addition. [Mh] Termos MeSH primário: Bacillus subtilis/enzimologia Proteínas de Bactérias/química Endo-1,4-beta-Xilanases/química Secale/química Streptomycetaceae/enzimologia Transglutaminases/química [Mh] Termos MeSH secundário: Pão/análise Culinária Farinha/análise Temperatura Alta [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Proteins); EC 2.3.2.13 (Transglutaminases); EC 3.2.1.8 (Endo-1,4-beta Xylanases) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170414 [Lr] Data última revisão: 170414 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160629 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.6b01545

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[PMID]: 27289100 [Au] Autor: Cruz-Morales P; Kopp JF; Martínez-Guerrero C; Yáñez-Guerra LA; Selem-Mojica N; Ramos-Aboites H; Feldmann J; Barona-Gómez F [Ad] Endereço: Evolution of Metabolic Diversity Laboratory, Langebio, Cinvestav-IPN, Irapuato, Guanajuato, México francisco.barona@cinvestav.mx cruzmoralesp@gmail.com. [Ti] Título: Phylogenomic Analysis of Natural Products Biosynthetic Gene Clusters Allows Discovery of Arseno-Organic Metabolites in Model Streptomycetes. [So] Source: Genome Biol Evol;8(6):1906-16, 2016 07 02. [Is] ISSN: 1759-6653 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Natural products from microbes have provided humans with beneficial antibiotics for millennia. However, a decline in the pace of antibiotic discovery exerts pressure on human health as antibiotic resistance spreads, a challenge that may better faced by unveiling chemical diversity produced by microbes. Current microbial genome mining approaches have revitalized research into antibiotics, but the empirical nature of these methods limits the chemical space that is explored.Here, we address the problem of finding novel pathways by incorporating evolutionary principles into genome mining. We recapitulated the evolutionary history of twenty-three enzyme families previously uninvestigated in the context of natural product biosynthesis in Actinobacteria, the most proficient producers of natural products. Our genome evolutionary analyses where based on the assumption that expanded-repurposed enzyme families-from central metabolism, occur frequently and thus have the potential to catalyze new conversions in the context of natural products biosynthesis. Our analyses led to the discovery of biosynthetic gene clusters coding for hidden chemical diversity, as validated by comparing our predictions with those from state-of-the-art genome mining tools; as well as experimentally demonstrating the existence of a biosynthetic pathway for arseno-organic metabolites in Streptomyces coelicolor and Streptomyces lividans, Using a gene knockout and metabolite profile combined strategy.As our approach does not rely solely on sequence similarity searches of previously identified biosynthetic enzymes, these results establish the basis for the development of an evolutionary-driven genome mining tool termed EvoMining that complements current platforms. We anticipate that by doing so real 'chemical dark matter' will be unveiled. [Mh] Termos MeSH primário: Farmacorresistência Fúngica/genética Evolução Molecular Genômica Streptomycetaceae/genética [Mh] Termos MeSH secundário: Antibacterianos/uso terapêutico Vias Biossintéticas/genética Genoma Bacteriano Seres Humanos Família Multigênica Streptomycetaceae/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170302 [Lr] Data última revisão: 170302 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160612 [St] Status: MEDLINE [do] DOI: 10.1093/gbe/evw125

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[PMID]: 27221142 [Au] Autor: Rashid AM; Batey SF; Syson K; Koliwer-Brandl H; Miah F; Barclay JE; Findlay KC; Nartowski KP; Khimyak YZ; Kalscheuer R; Bornemann S [Ad] Endereço: Biological Chemistry Department, John Innes Centre , Norwich Research Park, Norwich NR4 7UH, United Kingdom. [Ti] Título: Assembly of α-Glucan by GlgE and GlgB in Mycobacteria and Streptomycetes. [So] Source: Biochemistry;55(23):3270-84, 2016 Jun 14. [Is] ISSN: 1520-4995 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Actinomycetes, such as mycobacteria and streptomycetes, synthesize α-glucan with α-1,4 linkages and α-1,6 branching to help evade immune responses and to store carbon. α-Glucan is thought to resemble glycogen except for having shorter constituent linear chains. However, the fine structure of α-glucan and how it can be defined by the maltosyl transferase GlgE and branching enzyme GlgB were not known. Using a combination of enzymolysis and mass spectrometry, we compared the properties of α-glucan isolated from actinomycetes with polymer synthesized in vitro by GlgE and GlgB. We now propose the following assembly mechanism. Polymer synthesis starts with GlgE and its donor substrate, α-maltose 1-phosphate, yielding a linear oligomer with a degree of polymerization (∼16) sufficient for GlgB to introduce a branch. Branching involves strictly intrachain transfer to generate a C chain (the only constituent chain to retain its reducing end), which now bears an A chain (a nonreducing end terminal branch that does not itself bear a branch). GlgE preferentially extends A chains allowing GlgB to act iteratively to generate new A chains emanating from B chains (nonterminal branches that themselves bear a branch). Although extension and branching occur primarily with A chains, the other chain types are sometimes extended and branched such that some B chains (and possibly C chains) bear more than one branch. This occurs less frequently in α-glucans than in classical glycogens. The very similar properties of cytosolic and capsular α-glucans from Mycobacterium tuberculosis imply GlgE and GlgB are sufficient to synthesize them both. [Mh] Termos MeSH primário: Glucanos/química Glucanos/metabolismo Glucosiltransferases/metabolismo Mycobacterium/metabolismo Streptomycetaceae/metabolismo Fosfatos Açúcares/metabolismo [Mh] Termos MeSH secundário: Eletroforese Capilar Espectroscopia de Ressonância Magnética Mycobacterium/classificação Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Glucans); 0 (Sugar Phosphates); 15896-49-8 (maltose 1-phosphate); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.- (maltosyltransferase) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170502 [Lr] Data última revisão: 170502 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160526 [St] Status: MEDLINE [do] DOI: 10.1021/acs.biochem.6b00209

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[PMID]: 26877148 [Au] Autor: Li J; Wang M; Ding Y; Tang Y; Zhang Z; Chen Y [Ad] Endereço: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. [Ti] Título: Involvement of an octose ketoreductase and two acyltransferases in the biosynthesis of paulomycins. [So] Source: Sci Rep;6:21180, 2016 Feb 15. [Is] ISSN: 2045-2322 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: C-4 hydroxyethyl branched octoses have been observed in polysaccharides of several genera of gram negative bacteria and in various antibiotics produced by gram positive bacteria. The C-4 hydroxyethyl branch was proposed to be converted from C-4 acetyl branch by an uncharacterized ketoreduction step. Paulomycins (PAUs) are glycosylated antibiotics with potent inhibitory activity against gram positive bacteria and are structurally defined by its unique C-4' hydroxyethyl branched paulomycose moiety. A novel aldo-keto-reductase, Pau7 was characterized as the enzyme catalyzing the stereospecific ketoreduction of 7'-keto of PAU E (1) to give the C-4' hydroxyethyl branched paulomycose moiety of PAU F (2). An acyltransferase Pau6 further decorates the C-4' hydroxyethyl branch of paulomycose moiety of 2 by attaching various fatty acyl chains to 7'-OH to generate diverse PAUs. In addition, another acyltransferase Pau24 was proposed to be responsible for the 13-O-acetylation of PAUs. [Mh] Termos MeSH primário: Aciltransferases/química Antibacterianos/química Bactérias Gram-Positivas/efeitos dos fármacos Polissacarídeos/química [Mh] Termos MeSH secundário: Aciltransferases/biossíntese Aciltransferases/uso terapêutico Antibacterianos/biossíntese Antibacterianos/uso terapêutico Infecções por Chlamydia/tratamento farmacológico Infecções por Chlamydia/microbiologia Cicloexenos/química Dissacarídeos/biossíntese Dissacarídeos/química Bactérias Gram-Positivas/patogenicidade Oxirredutases/biossíntese Oxirredutases/química Polissacarídeos/biossíntese Polissacarídeos/uso terapêutico Streptomycetaceae/química [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Cyclohexenes); 0 (Disaccharides); 0 (Polysaccharides); 101411-69-2 (paulomycin E); 114413-27-3 (paulomycin F); EC 1.- (Oxidoreductases); EC 2.3.- (Acyltransferases) [Em] Mês de entrada: 1612 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160216 [St] Status: MEDLINE [do] DOI: 10.1038/srep21180

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[PMID]: 26675041 [Au] Autor: Wu C; Medema MH; Läkamp RM; Zhang L; Dorrestein PC; Choi YH; van Wezel GP [Ad] Endereço: Molecular Biotechnology, Institute of Biology, Leiden University , Sylviusweg 72, 2333 BE Leiden, The Netherlands. [Ti] Título: Leucanicidin and Endophenasides Result from Methyl-Rhamnosylation by the Same Tailoring Enzymes in Kitasatospora sp. MBT66. [So] Source: ACS Chem Biol;11(2):478-90, 2016 Feb 19. [Is] ISSN: 1554-8937 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The increasing bacterial multidrug resistance necessitates novel drug-discovery efforts. One way to obtain novel chemistry is glycosylation, which is prevalent in nature, with high diversity in both the sugar moieties and the targeted aglycones. Kitasatospora sp. MBT66 produces endophenaside antibiotics, which is a family of (methyl-)rhamnosylated phenazines. Here we show that this strain also produces the plecomacrolide leucanicidin (1), which is derived from bafilomycin A1 by glycosylation with the same methyl-rhamnosyl moiety as present in the endophenasides. Immediately adjacent to the baf genes for bafilomycin biosynthesis lie leuA and leuB, which encode a sugar-O-methyltransferase and a glycosyltransferase, respectively. LeuA and LeuB are the only enzymes encoded by the genome of Kitasatospora sp. MBT66 that are candidates for the methyl-rhamnosylation of natural products, and mutation of leuB abolished glycosylation of both families of natural products. Thus, LeuA and -B mediate the post-PKS methyl-rhamnosylation of bafilomycin A1 to leucanicidin and of phenazines to endophenasides, showing surprising promiscuity by tolerating both macrolide and phenazine skeletons as the substrates. Detailed metabolic analysis by MS/MS based molecular networking facilitated the characterization of nine novel phenazine glycosides 6-8, 16, and 22-26, whereby compounds 23 and 24 represent an unprecedented tautomeric glyceride phenazine, further enriching the structural diversity of endophenasides. [Mh] Termos MeSH primário: Antibacterianos/metabolismo Glicosiltransferases/metabolismo Macrolídeos/metabolismo Metiltransferases/metabolismo Fenazinas/metabolismo Streptomycetaceae/enzimologia [Mh] Termos MeSH secundário: Antibacterianos/química Glicosiltransferases/genética Macrolídeos/química Redes e Vias Metabólicas Metiltransferases/genética Família Multigênica Mutação Fenazinas/química Streptomycetaceae/química Streptomycetaceae/genética Streptomycetaceae/metabolismo Especificidade por Substrato Espectrometria de Massas em Tandem [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Macrolides); 0 (Phenazines); 0 (leucanicidin); 88899-55-2 (bafilomycin A1); EC 2.1.1.- (Methyltransferases); EC 2.4.- (Glycosyltransferases) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151218 [St] Status: MEDLINE [do] DOI: 10.1021/acschembio.5b00801

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[PMID]: 26852493 [Au] Autor: Vinogradoya A; Bulgakova VG; Polin AN; Kozhevin PA [Ti] Título: [On Biofilms of Streptomycetes. II. Use in Biotechnology]. [So] Source: Antibiot Khimioter;60(5-6):27-33, 2015. [Is] ISSN: 0235-2990 [Cp] País de publicação: Russia (Federation) [La] Idioma: rus [Ab] Resumo: Streptomycetes or mycelial microorganisms are able to form biofilms under the natural, industrial and clinical conditions. The controlled use of biofilms in various industrial processes is much more efficient vs. the cultivation of plankton suspended cells. Optimization of biotechnological processes with the use of streptomycete biofilms is advisable in production of lactic acid and detoxication of the liquor in pyrolysis of plant biomass. Streptomycete biofilms are used in water purification systems. It is recommended to use biofilms for detoxication of wastes and bioremediation of soils contaminated with hard metals. The use of biofilms of streptomycetes producing biologically active substances is of special interest. High yields of.antibiotics and actinomycin D in particular was observed with. cultivation of antibioc-producing streptomycetes as biofilms in bioreactors of unique design. [Mh] Termos MeSH primário: Biofilmes Biotecnologia/métodos Streptomycetaceae/fisiologia Purificação da Água/métodos [Mh] Termos MeSH secundário: Biodegradação Ambiental Biomassa [Pt] Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE; REVIEW [Em] Mês de entrada: 1602 [Cu] Atualização por classe: 161020 [Lr] Data última revisão: 161020 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160209 [St] Status: MEDLINE

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2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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