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[PMID]:29267499
[Au] Autor:Santos APP; Silva MDS; Costa EVL; Rufino RD; Santos VA; Ramos CS; Sarubbo LA; Porto ALF
[Ad] Endereço:Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil.
[Ti] Título:Production and characterization of a biosurfactant produced by Streptomyces sp. DPUA 1559 isolated from lichens of the Amazon region.
[So] Source:Braz J Med Biol Res;51(2):e6657, 2017 Dec 11.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Surfactants are amphipathic compounds containing both hydrophilic and hydrophobic groups, capable to lower the surface or interfacial tension. Considering the advantages of the use of biosurfactants produced by microorganisms, the aim of this paper was to develop and characterize a biosurfactant produced by Streptomyces sp. DPUA1559 isolated from lichens of the Amazon region. The microorganism was cultured in a mineral medium containing 1% residual frying soybean oil as the carbon source. The kinetics of biosurfactant production was accompanied by reducing the surface tension of the culture medium from 60 to values around 27.14 mN/m, and by the emulsification index, which showed the efficiency of the biosurfactant as an emulsifier of hydrophobic compounds. The yield of the isolated biosurfactant was 1.74 g/L, in addition to the excellent capability of reducing the surface tension (25.34 mN/m), as observed from the central composite rotational design when the biosurfactant was produced at pH 8.5 at 28°C. The critical micelle concentration of the biosurfactant was determined as 0.01 g/mL. The biosurfactant showed thermal and pH stability regarding the surface tension reduction, and tolerance under high salt concentrations. The isolated biosurfactant showed no toxicity to the micro-crustacean Artemia salina, and to the seeds of lettuce (Lactuca sativa L.) and cabbage (Brassica oleracea L.). The biochemistry characterization of the biosurfactant showed a single protein band, an acid character and a molecular weight around 14.3 kDa, suggesting its glycoproteic nature. The results are promising for the industrial application of this new biosurfactant.
[Mh] Termos MeSH primário: Líquens/microbiologia
Streptomyces/metabolismo
Tensoativos/metabolismo
[Mh] Termos MeSH secundário: Análise de Variância
Contagem de Colônia Microbiana
Meios de Cultura
Eletroforese em Gel de Poliacrilamida
Fermentação
Concentração de Íons de Hidrogênio
Valores de Referência
Sementes/efeitos dos fármacos
Óleo de Soja/química
Espectroscopia de Infravermelho com Transformada de Fourier
Streptomyces/crescimento & desenvolvimento
Streptomyces/isolamento & purificação
Tensão Superficial
Tensoativos/análise
Tensoativos/química
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Surface-Active Agents); 8001-22-7 (Soybean Oil)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE


  2 / 16213 MEDLINE  
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[PMID]:28461449
[Au] Autor:Stubbendieck RM; Straight PD
[Ad] Endereço:Interdisciplinary Program in Genetics, Texas A&M University, College Station, Texas, USA.
[Ti] Título:Linearmycins Activate a Two-Component Signaling System Involved in Bacterial Competition and Biofilm Morphology.
[So] Source:J Bacteriol;199(18), 2017 09 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria use two-component signaling systems to adapt and respond to their competitors and changing environments. For instance, competitor bacteria may produce antibiotics and other bioactive metabolites and sequester nutrients. To survive, some species of bacteria escape competition through antibiotic production, biofilm formation, or motility. Specialized metabolite production and biofilm formation are relatively well understood for bacterial species in isolation. How bacteria control these functions when competitors are present is not well studied. To address fundamental questions relating to the competitive mechanisms of different species, we have developed a model system using two species of soil bacteria, and sp. strain Mg1. Using this model, we previously found that linearmycins produced by sp. strain Mg1 cause lysis of cells and degradation of colony matrix. We identified strains of with mutations in the two-component signaling system operon that confer dual phenotypes of specific linearmycin resistance and biofilm morphology. We determined that expression of the ATP-binding cassette (ABC) transporter operon, particularly and , is necessary for biofilm morphology. Using transposon mutagenesis, we identified genes that are required for YfiLMN-mediated biofilm morphology, including several chaperones. Using transcriptional fusions, we found that YfiJ signaling is activated by linearmycins and other polyene metabolites. Finally, using a truncated YfiJ, we show that YfiJ requires its transmembrane domain to activate downstream signaling. Taken together, these results suggest coordinated dual antibiotic resistance and biofilm morphology by a single multifunctional ABC transporter promotes competitive fitness of DNA sequencing approaches have revealed hitherto unexplored diversity of bacterial species in a wide variety of environments that includes the gastrointestinal tract of animals and the rhizosphere of plants. Interactions between different species in bacterial communities have impacts on our health and industry. However, many approaches currently used to study whole bacterial communities do not resolve mechanistic details of interspecies interactions, including how bacteria sense and respond to their competitors. Using a competition model, we have uncovered dual functions for a previously uncharacterized two-component signaling system involved in specific antibiotic resistance and biofilm morphology. Insights gleaned from signaling within interspecies interaction models build a more complete understanding of gene functions important for bacterial communities and will enhance community-level analytical approaches.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Antibiose
Bacillus subtilis/fisiologia
Biofilmes/crescimento & desenvolvimento
Viabilidade Microbiana
Streptomyces/fisiologia
[Mh] Termos MeSH secundário: Transportadores de Cassetes de Ligação de ATP/metabolismo
Fusão Gênica Artificial
Bacillus subtilis/efeitos dos fármacos
Elementos de DNA Transponíveis
Perfilação da Expressão Gênica
Regulação Bacteriana da Expressão Gênica
Mutagênese Insercional
Mutação
Transdução de Sinais
Streptomyces/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (ATP-Binding Cassette Transporters); 0 (Anti-Bacterial Agents); 0 (DNA Transposable Elements)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:28449057
[Au] Autor:Schumacher MA; Zeng W; Findlay KC; Buttner MJ; Brennan RG; Tschowri N
[Ad] Endereço:Department of Biochemistry, Duke University School of Medicine, Durham, NC 27701, USA.
[Ti] Título:The Streptomyces master regulator BldD binds c-di-GMP sequentially to create a functional BldD2-(c-di-GMP)4 complex.
[So] Source:Nucleic Acids Res;45(11):6923-6933, 2017 Jun 20.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Streptomyces are ubiquitous soil bacteria that undergo a complex developmental transition coinciding with their production of antibiotics. This transition is controlled by binding of a novel tetrameric form of the second messenger, 3΄-5΄ cyclic diguanylic acid (c-di-GMP) to the master repressor, BldD. In all domains of life, nucleotide-based second messengers allow a rapid integration of external and internal signals into regulatory pathways that control cellular responses to changing conditions. c-di-GMP can assume alternative oligomeric states to effect different functions, binding to effector proteins as monomers, intercalated dimers or, uniquely in the case of BldD, as a tetramer. However, at physiological concentrations c-di-GMP is a monomer and little is known about how higher oligomeric complexes assemble on effector proteins and if intermediates in assembly pathways have regulatory significance. Here, we show that c-di-GMP binds BldD using an ordered, sequential mechanism and that BldD function necessitates the assembly of the BldD2-(c-di-GMP)4 complex.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
GMP Cíclico/análogos & derivados
Proteínas Repressoras/química
Streptomyces
[Mh] Termos MeSH secundário: Sítios de Ligação
Cristalografia por Raios X
GMP Cíclico/química
Ligações de Hidrogênio
Modelos Moleculares
Ligação Proteica
Domínios Proteicos
Estabilidade Proteica
Estrutura Quaternária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Repressor Proteins); 61093-23-0 (bis(3',5')-cyclic diguanylic acid); H2D2X058MU (Cyclic GMP)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx287


  4 / 16213 MEDLINE  
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[PMID]:27778478
[Au] Autor:Hoang NH; Huong NL; Kim B; Sohng JK; Yoon YJ; Park JW
[Ad] Endereço:Department of Biotechnology Convergent Pharmaceutical Engineering, SunMoon University, Chungnam, Republic of Korea.
[Ti] Título:Istamycin aminoglycosides profiling and their characterization in Streptomyces tenjimariensis ATCC 31603 culture using high-performance liquid chromatography with tandem mass spectrometry.
[So] Source:J Sep Sci;39(24):4712-4722, 2016 Dec.
[Is] ISSN:1615-9314
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A high-performance liquid chromatography with electrospray ionization ion trap tandem mass spectrometry method was developed and validated for the robust profiling and characterization of biosynthetic congeners in the 2-deoxy-aminocyclitol istamycin pathway, from the fermentation broth of Streptomyces tenjimariensis ATCC 31603. Gradient elution on an Acquity CSH C column was performed with a gradient of 5 mM aqueous pentafluoropropionic acid and 50% acetonitrile. Sixteen natural istamycin congeners were profiled and quantified in descending order; istamycin A, istamycin B, istamycin A , istamycin B , istamycin B , istamycin A , istamycin C, istamycin A , istamycin C , istamycin C , istamycin X , istamycin A , istamycin Y , istamycin B , and istamycin FU-10 plus istamycin AP. In addition, a total of five sets of 1- or 3-epimeric pairs were chromatographically separated using a macrocyclic glycopeptide-bonded chiral column. The lower limit of quantification of istamycin-A present in S. tenjimariensis fermentation was estimated to be 2.2 ng/mL. The simultaneous identification of a wide range of 2-deoxy-aminocyclitol-type istamycin profiles from bacterial fermentation was determined for the first time by employing high-performance liquid chromatography with tandem mass spectrometry analysis and the separation of istamycin epimers.
[Mh] Termos MeSH primário: Aminoglicosídeos/biossíntese
Antibacterianos/biossíntese
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aminoglycosides); 0 (Anti-Bacterial Agents); 76774-97-5 (istamycins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1002/jssc.201600925


  5 / 16213 MEDLINE  
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[PMID]:28923725
[Au] Autor:Baoune H; Ould El Hadj-Khelil A; Pucci G; Sineli P; Loucif L; Polti MA
[Ad] Endereço:Laboratoire de protection des écosystème en zones arides et semi-arides, FNSV, Université Kasdi Merbah Ouragla, 30000, Algeria. Electronic address: baounehafida@hotmail.fr.
[Ti] Título:Petroleum degradation by endophytic Streptomyces spp. isolated from plants grown in contaminated soil of southern Algeria.
[So] Source:Ecotoxicol Environ Saf;147:602-609, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Petroleum hydrocarbons are well known by their high toxicity and recalcitrant properties. Their increasing utilization around worldwide led to environmental contamination. Phytoremediation using plant-associated microbe is an interesting approach for petroleum degradation and actinobacteria have a great potential for that. For this purpose, our study aimed to isolate, characterize, and assess the ability of endophytic actinobacteria to degrade crude petroleum, as well as to produce plant growth promoting traits. Seventeen endophytic actinobacteria were isolated from roots of plants grown naturally in sandy contaminated soil. Among them, six isolates were selected on the basis of their tolerance to petroleum on solid minimal medium and characterized by 16S rDNA gene sequencing. All petroleum-tolerant isolates belonged to the Streptomyces genus. Determination by crude oil degradation by gas chromatorgraph-flame ionization detector revealed that five strains could use petroleum as sole carbon and energy source and the petroleum removal achieved up to 98% after 7 days of incubation. These isolates displayed an important role in the degradation of the n-alkanes (C -C ), aromatic and polycyclic aromatic hydrocarbons. All strains showed a wide range of plant growth promoting features such as siderophores, phosphate solubilization, 1-aminocyclopropane-1-carboxylate deaminase, nitrogen fixation and indole-3-acetic acid production as well as biosurfactant production. This is the first study highlighting the petroleum degradation ability and plant growth promoting attributes of endophytic Streptomyces. The finding suggests that the endophytic actinobacteria isolated are promising candidates for improving phytoremediation efficiency of petroleum contaminated soil.
[Mh] Termos MeSH primário: Endófitos/metabolismo
Petróleo/análise
Plantas/microbiologia
Poluentes do Solo/análise
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Argélia
Biodegradação Ambiental
Carbono-Carbono Liases/metabolismo
Endófitos/crescimento & desenvolvimento
Fixação de Nitrogênio/efeitos dos fármacos
Desenvolvimento Vegetal/efeitos dos fármacos
Plantas/metabolismo
Solo/química
Streptomyces/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Petroleum); 0 (Soil); 0 (Soil Pollutants); EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase); EC 4.1.- (Carbon-Carbon Lyases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


  6 / 16213 MEDLINE  
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[PMID]:29251849
[Au] Autor:Kumar S; Priya E; Singh Solanki D; Sharma R; Gehlot P; Pathak R; Singh SK
[Ti] Título:Occurrence and characterization of hitherto unknown Streptomyces species in semi-arid soils.
[So] Source:J Environ Biol;37(5):927-36, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Streptomyces the predominant genus of Actinobacteria and plays an important role in the recycling of soil organic matter and production of important secondary metabolites. The occurrence and diversity assessment of Streptomyces species revealed alkaline and poor nutrient status of soils of semi-arid region of Jodhpur, Rajasthan. The morphological and biochemical characterization of 21 Streptomyces isolates facilitated Genus level identification but were insufficient to designate species. Species designation based on 16S rRNA gene delineated 21 isolates into 14 Streptomyces species. Upon BLAST search, the test isolates exhibited 98 to 100% identities with that of the best aligned sequences of the NCBI database. The GC content of 16S rRNA gene sequences of all the Streptomyces isolates tested ranged from 59.03% to 60.94%. The multiple sequence alignment of all the 21 Streptomyces isolates generated a phylogram with high bootstrap values indicating reliable grouping of isolates based on nucleotide sequence variations by way of insertion, deletion and substitutions and 16S rRNA length polymorphism. Some of the Streptomyces species molecularly identified under present study are reported for the first time from semi-arid region of Jodhpur.
[Mh] Termos MeSH primário: Streptomyces/genética
Streptomyces/isolamento & purificação
[Mh] Termos MeSH secundário: DNA Bacteriano/genética
Índia
Técnicas de Amplificação de Ácido Nucleico
Filogenia
Polimorfismo Genético
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Microbiologia do Solo
Especificidade da Espécie
[Pt] Tipo de publicação:RESEARCH SUPPORT, NON-U.S. GOV'T; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  7 / 16213 MEDLINE  
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[PMID]:29257362
[Au] Autor:Sharma P; Choudhary B; Nagpure A; Gupta RK
[Ti] Título:Antifungal potential of actinomycete isolate Streptomyces exfoliates MT9 against wood-rotting fungi.
[So] Source:J Environ Biol;37(6):1231-37, 2016 Nov.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:An actinomycete isolate, Streptomyces exfoliatus MT9 was assessed for in vitro antagonism against wood-rotting fungi. Strain MT9 showed strong antagonistic activity (ZOI ? 25 mm) towards various tested wood-rotting fungi. Extracellular production of antifungal metabolite(s) including primary and secondary was monitored up to 10 days of submerged fermentation. Antagonist S. exfoliatus MT9 produces fungal cell-wall lytic enzymes, namely chitinase (3.098 U ml-1), b-1,3 glucanase (2.4 U ml-1) and protease (144.0 U ml-1) and also showed antifungal activity towards tested P. chrysosporium MTCC 787 (12.0 mm) and P. placenta MTCC 144 (16.0 mm). Extracellular culture filtrate (ECF) of S. exfoliatus MT9 also exhibited strong antifungal activity (ZOI ≥ 25 mm) towards tested wood-rotting fungi and n-butanol was found to be the suitable solvent for complete extraction of antifungal metabolite(s) from ECF. Reduced antifungal activity of n-butanol extract against P. chrysosporium MTCC 787 (11.00 mm) and P. placenta MTCC 144 (10.00 mm) on ergosterol agar plate, no activity against bacteria and characteristic UV spectra at 224 nm revealed the polyene nature of antifungal metabolite(s) present in the n-butanol extract. A novel actinomycete isolate, S. exfoliatus MT9 is producing antifungal metabolite(s) that makes it suitable for biotechnological processes and has the potential to be used as a bioactive agent for controlling wood-rotting fungi.
[Mh] Termos MeSH primário: Proteínas de Bactérias/farmacologia
Quitinases/metabolismo
Quitinases/farmacologia
Fungos/fisiologia
Streptomyces/enzimologia
Madeira/microbiologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Parede Celular/efeitos dos fármacos
Regulação Bacteriana da Expressão Gênica
Regulação Enzimológica da Expressão Gênica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 3.2.1.14 (Chitinases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  8 / 16213 MEDLINE  
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[PMID]:29257360
[Au] Autor:Abid I; Mujamammi R; Alkahtani MDF
[Ti] Título:Antimicrobial activity and molecular identification of Streptomyces strains isolated from Saudi Arabia.
[So] Source:J Environ Biol;37(6):1225-30, 2016 Nov.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Actinomycetes are group of Gram-positive bacteria with high GC-content in their DNA. They are extremely useful for the pharmaceutical industry due to their seemingly unlimited capacity to produce secondary metabolites with diverse biological activities and chemical structure. The genus Streptomyces constitutes 50% of the total population of soil actinomycetes and about 75% of commercially and medicinally useful antibiotics that have been derived from this genus. The present study aimed in isolation of bioactive compound showing antimicrobial activities from soil Streptomyces, previously isolated and morphologically characterized from Jazan in Saudi Arabia. Six potent Streptomyces strains: JS3, JS4, JS6, JD7, JA8 and JA10 were chosen for antimicrobial activity screening against 5 human pathogenic bacteria and 5 phytopathogenic fungi before molecular identification was done. For antibacterial activity, the results showed that inhibition zones were found to range between 3.25-26.875 mm diameters, while for antifungal activity, it ranged between 13.3-40 mm diameters. The entire sequence of the 16S rDNA was determined for the strains JS6, JD7, JA8 and JA10 and deposited in the GenBank. Future studies of actinomycetes isolated from the Kingdom of Saudi Arabia's soils will assist in the discovery of new compounds that would be of industrial, pharmaceutical and agricultural importance.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
DNA Bacteriano/genética
Avaliação Pré-Clínica de Medicamentos
Filogenia
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Arábia Saudita
Microbiologia do Solo
Streptomyces/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  9 / 16213 MEDLINE  
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[PMID]:29324854
[Au] Autor:Suroto DA; Kitani S; Arai M; Ikeda H; Nihira T
[Ad] Endereço:International Center for Biotechnology, Osaka University, Suita, Osaka, Japan.
[Ti] Título:Characterization of the biosynthetic gene cluster for cryptic phthoxazolin A in Streptomyces avermitilis.
[So] Source:PLoS One;13(1):e0190973, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phthoxazolin A, an oxazole-containing polyketide, has a broad spectrum of anti-oomycete activity and herbicidal activity. We recently identified phthoxazolin A as a cryptic metabolite of Streptomyces avermitilis that produces the important anthelmintic agent avermectin. Even though genome data of S. avermitilis is publicly available, no plausible biosynthetic gene cluster for phthoxazolin A is apparent in the sequence data. Here, we identified and characterized the phthoxazolin A (ptx) biosynthetic gene cluster through genome sequencing, comparative genomic analysis, and gene disruption. Sequence analysis uncovered that the putative ptx biosynthetic genes are laid on an extra genomic region that is not found in the public database, and 8 open reading frames in the extra genomic region could be assigned roles in the biosynthesis of the oxazole ring, triene polyketide and carbamoyl moieties. Disruption of the ptxA gene encoding a discrete acyltransferase resulted in a complete loss of phthoxazolin A production, confirming that the trans-AT type I PKS system is responsible for the phthoxazolin A biosynthesis. Based on the predicted functional domains in the ptx assembly line, we propose the biosynthetic pathway of phthoxazolin A.
[Mh] Termos MeSH primário: Álcoois Graxos/metabolismo
Genes Bacterianos
Família Multigênica
Oxazóis/metabolismo
Alcamidas Poli-Insaturadas/metabolismo
Streptomyces/genética
[Mh] Termos MeSH secundário: Álcoois Graxos/química
Modelos Biológicos
Oxazóis/química
Alcamidas Poli-Insaturadas/química
Streptomyces/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Alcohols); 0 (Oxazoles); 0 (Polyunsaturated Alkamides); 130288-22-1 (phthoxazolin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190973


  10 / 16213 MEDLINE  
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[PMID]:29227220
[Au] Autor:Landwehr W; Kämpfer P; Glaeser SP; Rückert C; Kalinowski J; Blom J; Goesmann A; Mack M; Schumann P; Atasayar E; Hahnke RL; Rohde M; Martin K; Stadler M; Wink J
[Ad] Endereço:1​Department of Microbial Strain Collection, Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
[Ti] Título:Taxonomic analyses of members of the Streptomyces cinnabarinus cluster, description of Streptomyces cinnabarigriseus sp. nov. and Streptomyces davaonensis sp. nov.
[So] Source:Int J Syst Evol Microbiol;68(1):382-393, 2018 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Roseoflavin is the only known riboflavin (vitamin B2) analog with antibiotic properties. It is actively taken up by many micro-organisms and targets flavinmononucleotide riboswitches and flavoproteins. It is described as the product of the tentatively named 'Streptomyces davawensis' JCM 4913. Taxonomic analysis of this strain with a polyphasic approach showed that it is very closely related to Streptomyces cinnabarinus (DSM 40467). The two Streptomyces isolates were obtained from different geographical locations (the Philippines and the Kamchatka Peninsula, respectively), their genomes have been sequenced and the question was whether or not the two isolates were representatives of the same species. As we also worked with another isolate of Streptomyces cinnabarinus JS 360, the producer of the cinnabaramides, we wanted to clarify the taxonomic position of the three isolates by using a polyphasic approach. After analysis of the 16S rRNA gene sequence, we found in total 23 species of the genus Streptomyces that showed a similarity higher than 98.5 % to the three strains. We showed that 'S. davawensis' JCM 4913 and S. cinnabarinus DSM 40467 were very closely related but belong to two different species. Hence, we validate 'S. davawensis' as Streptomyces davaonensis sp. nov. with the type strain JCM 4913 (=DSM 101723 ). In addition, the cinnabaramide producer can be clearly differentiated from S. davaonensis and this isolate is described as Streptomyces cinnabarigriseus sp. nov. with strain JS360 (=NCCB 100590 =DSM 101724 ) as the type strain.
[Mh] Termos MeSH primário: Filogenia
Streptomyces/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
Filipinas
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002519



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