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  1 / 1845 MEDLINE  
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[PMID]:29458479
[Au] Autor:Núñez-Montero K; Leclercq A; Moura A; Vales G; Peraza J; Pizarro-Cerdá J; Lecuit M
[Ad] Endereço:1​Centro de Investigación en Biotecnología, Escuela de Biología, Instituto Tecnológico de Costa Rica, Cartago, Costa Rica.
[Ti] Título:Listeria costaricensis sp. nov.
[So] Source:Int J Syst Evol Microbiol;68(3):844-850, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bacterial strain isolated from a food processing drainage system in Costa Rica fulfilled the criteria as belonging to the genus Listeria, but could not be assigned to any of the known species. Phylogenetic analysis based on the 16S rRNA gene revealed highest sequence similarity with the type strain of Listeria floridensis (98.7 %). Phylogenetic analysis based on Listeria core genomes placed the novel taxon within the Listeria fleishmannii, L. floridensis and Listeria aquatica clade (Listeria sensu lato). Whole-genome sequence analyses based on the average nucleotide blast identity (ANI<80 %) indicated that this isolate belonged to a novel species. Results of pairwise amino acid identity (AAI>70 %) and percentage of conserved proteins (POCP>68 %) with currently known Listeria species, as well as of biochemical characterization, confirmed that the strain constituted a novel species within the genus Listeria. The name Listeria costaricensis sp. nov. is proposed for the novel species, and is represented by the type strain CLIP 2016/00682 (=CIP 111400 =DSM 105474 ).
[Mh] Termos MeSH primário: Indústria Alimentícia
Listeria/classificação
Filogenia
Águas Residuais/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Costa Rica
DNA Bacteriano/genética
Listeria/genética
Listeria/isolamento & purificação
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Waste Water)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002596


  2 / 1845 MEDLINE  
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ALVARENGA, Elson Santiago
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[PMID]:29286652
[Au] Autor:Pinheiro PF; Menini LAP; Bernardes PC; Saraiva SH; Carneiro JWM; Costa AV; Arruda TR; Lage MR; Gonçalves PM; Bernardes CO; Alvarenga ES; Menini L
[Ti] Título:Semisynthetic Phenol Derivatives Obtained from Natural Phenols: Antimicrobial Activity and Molecular Properties.
[So] Source:J Agric Food Chem;66(1):323-330, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Semisynthetic phenol derivatives were obtained from the natural phenols: thymol, carvacrol, eugenol, and guaiacol through catalytic oxychlorination, Williamson synthesis, and aromatic Claisen rearrangement. The compounds characterization was carried out by H NMR, C NMR, and mass spectrometry. The natural phenols and their semisynthetic derivatives were tested for their antimicrobial activity against the bacteria: Staphylococcus aureus, Escherichia coli, Listeria innocua, Pseudomonas aeruginosa, Salmonella enterica Typhimurium, Salmonella enterica ssp. enterica, and Bacillus cereus. Minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values were determined using concentrations from 220 to 3.44 µg mL . Most of the tested compounds presented MIC values ≤220 µg mL for all the bacteria used in the assays. The molecular properties of the compounds were computed with the PM6 method. Through principle components analysis, the natural phenols and their semisynthetic derivatives with higher antimicrobial potential were grouped.
[Mh] Termos MeSH primário: Antibacterianos/síntese química
Antibacterianos/farmacologia
Fenol/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Escherichia coli/efeitos dos fármacos
Eugenol/química
Eugenol/farmacologia
Listeria/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Monoterpenos/química
Monoterpenos/farmacologia
Fenol/síntese química
Fenol/química
Salmonella typhimurium/efeitos dos fármacos
Staphylococcus aureus/efeitos dos fármacos
Timol/química
Timol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Monoterpenes); 339NCG44TV (Phenol); 3J50XA376E (Thymol); 3T8H1794QW (Eugenol); 9B1J4V995Q (carvacrol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04418


  3 / 1845 MEDLINE  
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[PMID]:28945113
[Au] Autor:Liu D; Wang Y; Wang Y; Zhang L; Luo L; Liu K; Ye C
[Ad] Endereço:State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, Changbai Road 155,
[Ti] Título:Development of a Novel Listeria Enrichment Broth for the Isolation of Pathogenic Listeria.
[So] Source:J Food Prot;80(10):1768-1776, 2017 Oct.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Listeriosis, the disease caused by pathogenic Listeria species, can present severe symptoms in susceptible people. The goal of this study was to develop a novel enrichment broth, Listeria allose enrichment broth (LAEB), to improve isolation of Listeria monocytogenes and Listeria ivanovii from samples through incorporating a specific carbohydrate and reducing inhibitor concentrations. Other coexisting bacteria, particularly Listeria innocua, can interfere with the isolation of pathogenic Listeria in such ways as overgrowth of L. innocua and the generation of inhibitory metabolites. The incorporation of allose into the novel LAEB was effective for slowing the growth of L. innocua and other nontarget microorganisms. We determined that 35°C and pH 7.0 under aerobic conditions are optimal for Listeria growth in this medium. The novelty of the use of LAEB is the single enrichment procedure at 35°C for 24 h, obviating the need for a secondary enrichment medium. In 50 simulated samples, the sensitivity of the LAEB method (86%) was higher than that of the International Organization for Standardization (EN ISO) method (70%). In 142 naturally contaminated samples tested, the isolation rate for pathogenic Listeria with the LAEB method was 26.0% (37 of 142 samples), which was significantly higher than the 17.6% (25 of 142 samples) for the EN ISO method. Higher isolation rates and a quicker and easier protocol make the novel LAEB method an appropriate alternative for the isolation of pathogenic Listeria.
[Mh] Termos MeSH primário: Meios de Cultura
Microbiologia de Alimentos
Listeria/isolamento & purificação
[Mh] Termos MeSH secundário: Seres Humanos
Listeria monocytogenes
Listeriose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-529


  4 / 1845 MEDLINE  
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[PMID]:28912268
[Au] Autor:Shen Y; Boulos S; Sumrall E; Gerber B; Julian-Rodero A; Eugster MR; Fieseler L; Nyström L; Ebert MO; Loessner MJ
[Ad] Endereço:From the Laboratory of Food Microbiology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, CH-8092 Zurich, yang.shen@hest.ethz.ch.
[Ti] Título:Structural and functional diversity in cell wall teichoic acids.
[So] Source:J Biol Chem;292(43):17832-17844, 2017 Oct 27.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wall teichoic acids (WTAs) are the most abundant glycopolymers found on the cell wall of many Gram-positive bacteria, whose diverse surface structures play key roles in multiple biological processes. Despite recent technological advances in glycan analysis, structural elucidation of WTAs remains challenging due to their complex nature. Here, we employed a combination of ultra-performance liquid chromatography-coupled electrospray ionization tandem-MS/MS and NMR to determine the structural complexity of WTAs from species. We unveiled more than 10 different types of WTA polymers that vary in their linkage and repeating units. Disparity in GlcNAc to ribitol connectivity, as well as variable -acetylation and glycosylation of GlcNAc contribute to the structural diversity of WTAs. Notably, SPR analysis indicated that constitution of WTA determines the recognition by bacteriophage endolysins. Collectively, these findings provide detailed insight into cell wall-associated carbohydrates, and will guide further studies on the structure-function relationship of WTAs.
[Mh] Termos MeSH primário: Parede Celular/química
Parede Celular/metabolismo
Listeria/metabolismo
Ácidos Teicoicos/química
Ácidos Teicoicos/metabolismo
[Mh] Termos MeSH secundário: Acetilglucosamina/química
Acetilglucosamina/metabolismo
Ribitol/química
Ribitol/metabolismo
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Teichoic Acids); 488-81-3 (Ribitol); V956696549 (Acetylglucosamine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.813964


  5 / 1845 MEDLINE  
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[PMID]:28757183
[Au] Autor:Sherratt AR; Rouleau Y; Luebbert C; Strmiskova M; Veres T; Bidawid S; Corneau N; Pezacki JP
[Ad] Endereço:Department of Chemistry and Biomolecular Sciences, Centre for Chemical and Synthetic Biology, University of Ottawa, 10 Marie-Curie Private, Ottawa K1N 6N5, Canada; Life Sciences Division, National Research Council of Canada, 100 Sussex Drive, Ottawa K1A 0R6, Canada.
[Ti] Título:Rapid Screening and Identification of Living Pathogenic Organisms via Optimized Bioorthogonal Non-canonical Amino Acid Tagging.
[So] Source:Cell Chem Biol;24(8):1048-1055.e3, 2017 Aug 17.
[Is] ISSN:2451-9456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pathogenic bacteria can be a major cause of illness from environmental sources as well as the consumption of contaminated products, giving rise to public health concerns globally. The surveillance of such living organisms in food and water supplies remains an important challenge in mitigating their deleterious societal effects. Here, we have developed an optimized bioorthogonal non-canonical amino acid tagging approach to the imaging, capture, and interrogation of shigatoxigenic/verotoxigenic Escherichia coli (VTEC) and Listeria that enables the distinction between living wild-type pathogenic bacteria. The approaches utilize homopropargylglycine (HPG), as well as optimized growth media, that restricts endogenous methionine biosynthesis in a variety of species of public health concern. Endogenous methionine residues are then replaced with HPG, which can then be modified using a myriad of compatible bioorthogonal reactions for tagging of exclusively live bacteria. The methods reported allow for the very rapid screening and identification of living pathogenic organisms.
[Mh] Termos MeSH primário: Aminoácidos/metabolismo
Escherichia coli/isolamento & purificação
Listeria/isolamento & purificação
[Mh] Termos MeSH secundário: Alquinos/química
Alquinos/metabolismo
Aminoácidos/química
Azidas/química
Cobre/química
Reação de Cicloadição
Escherichia coli/metabolismo
Microbiologia de Alimentos
Glicina/análogos & derivados
Glicina/química
Glicina/metabolismo
Seres Humanos
Listeria/metabolismo
Microscopia de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkynes); 0 (Amino Acids); 0 (Azides); 0 (homopropargylglycine); 789U1901C5 (Copper); TE7660XO1C (Glycine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE


  6 / 1845 MEDLINE  
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[PMID]:28549184
[Au] Autor:Gupta K; Sharp R; Yuan JB; Li H; Van Duyne GD
[Ad] Endereço:Department of Biochemistry & Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 10104, USA.
[Ti] Título:Coiled-coil interactions mediate serine integrase directionality.
[So] Source:Nucleic Acids Res;45(12):7339-7353, 2017 Jul 07.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Serine integrases are bacteriophage enzymes that carry out site-specific integration and excision of their viral genomes. The integration reaction is highly directional; recombination between the phage attachment site attP and the host attachment site attB to form the hybrid sites attL and attR is essentially irreversible. In a recent model, extended coiled-coil (CC) domains in the integrase subunits are proposed to interact in a way that favors the attPxattB reaction but inhibits the attLxattR reaction. Here, we show for the Listeria innocua integrase (LI Int) system that the CC domain promotes self-interaction in isolated Int and when Int is bound to attachment sites. Three independent crystal structures of the CC domain reveal the molecular nature of the CC dimer interface. Alanine substitutions of key residues in the interface support the functional significance of the structural model and indicate that the same interaction is responsible for promoting integration and for inhibiting excision. An updated model of a LI Int•attL complex that incorporates the high resolution CC dimer structure provides insights that help to explain the unusual CC dimer structure and potential sources of stability in Int•attL and Int•attR complexes. Together, the data provide a molecular basis for understanding serine integrase directionality.
[Mh] Termos MeSH primário: Sítios de Ligação Microbiológicos
Bacteriófagos/genética
DNA Bacteriano/química
Integrases/química
Listeria/virologia
Serina/química
Proteínas Virais/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Bacteriófagos/metabolismo
Sítios de Ligação
Clonagem Molecular
Cristalografia por Raios X
DNA Bacteriano/genética
DNA Bacteriano/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Integrases/genética
Integrases/metabolismo
Cinética
Listeria/genética
Listeria/metabolismo
Modelos Moleculares
Mutagênese Insercional
Ligação Proteica
Conformação Proteica em alfa-Hélice
Domínios e Motivos de Interação entre Proteínas
Multimerização Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Recombinação Genética
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Serina/metabolismo
Especificidade por Substrato
Termodinâmica
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Recombinant Proteins); 0 (Viral Proteins); 452VLY9402 (Serine); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx474


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[PMID]:28411150
[Au] Autor:Suryawanshi RD; Malik SVS; Jayarao B; Chaudhari SP; Savage E; Vergis J; Kurkure NV; Barbuddhe SB; Rawool DB
[Ad] Endereço:Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, India.
[Ti] Título:Comparative diagnostic efficacy of recombinant LLO and PI-PLC-based ELISAs for detection of listeriosis in animals.
[So] Source:J Microbiol Methods;137:40-45, 2017 Jun.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and phosphatidyl-inositol phospholipase C (rPI-PLC). Indirect ELISA with the above recombinant antigens was used on samples collected from bovines (n=106), goats (n=138) and pigs (n=92) having either a history of abortion, emaciation and/or apparently healthy animals. Isolation of Listeria was attempted from the blood samples using USDA-FSIS method. On screening of test sera by rLLO-based ELISA, antibodies against anti-listeriolysin O (ALLO) were observed in goats (22.46%), bovines (15.10%) and pigs (16.31%). As advocated, after adsorption of positive serum samples with streptolysin O (SLO), the seropositivity for ALLO was marginally reduced (p>0.05) in goats (21.73%) and bovines (10.38%), whereas, in pigs the reduction (5.43%) was significant (p<0.05). On the contrary, rPI-PLC-based ELISA revealed higher non-specific seropositivity for antilisterial antibodies in goats (45.65%), bovines (31.13%) and pigs (8.69%). Further, on comparing the seropositivity with isolation rate, of the 16 animals that were culturally-positive for L. monocytogenes, 15 showed ALLO positivity in unadsorbed as well as SLO-adsorbed sera by rLLO-based ELISA, however, rPI-PLC-based ELISA could detect seropositivity in only 5 animals. Moreover, rPI-PLC-based ELISA also showed seropositivity in those animals (7/30) that were culturally positive for other Listeria spp. In conclusion, rLLO can serve as a better antigen than rPI-PLC in ELISA for the serodiagnosis of listeriosis in animals; however, prior adsorption of test sera with SLO is required to avoid false positive results.
[Mh] Termos MeSH primário: Doenças dos Animais/microbiologia
Toxinas Bacterianas/análise
Ensaio de Imunoadsorção Enzimática/veterinária
Proteínas de Choque Térmico/análise
Proteínas Hemolisinas/análise
Listeriose/veterinária
Fosfoinositídeo Fosfolipase C/análise
[Mh] Termos MeSH secundário: Doenças dos Animais/sangue
Doenças dos Animais/diagnóstico
Animais
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/genética
Proteínas de Bactérias/sangue
Toxinas Bacterianas/genética
Toxinas Bacterianas/imunologia
Bovinos
Ensaio de Imunoadsorção Enzimática/métodos
Cabras
Proteínas de Choque Térmico/genética
Proteínas de Choque Térmico/imunologia
Proteínas Hemolisinas/genética
Proteínas Hemolisinas/imunologia
Listeria/enzimologia
Listeria/isolamento & purificação
Listeriose/sangue
Listeriose/diagnóstico
Listeriose/imunologia
Fosfoinositídeo Fosfolipase C/genética
Fosfoinositídeo Fosfolipase C/imunologia
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Sensibilidade e Especificidade
Testes Sorológicos/métodos
Testes Sorológicos/veterinária
Estreptolisinas/sangue
Suínos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (Heat-Shock Proteins); 0 (Hemolysin Proteins); 0 (Recombinant Proteins); 0 (Streptolysins); 0 (streptolysin O); 72270-41-8 (hlyA protein, Listeria monocytogenes); EC 3.1.4.11 (Phosphoinositide Phospholipase C)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE


  8 / 1845 MEDLINE  
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[PMID]:28319798
[Au] Autor:Jiang Y; Sokorai K; Pyrgiotakis G; Demokritou P; Li X; Mukhopadhyay S; Jin T; Fan X
[Ad] Endereço:Key Laboratory of Food Nutrition and Safety (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457, China.
[Ti] Título:Cold plasma-activated hydrogen peroxide aerosol inactivates Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria innocua and maintains quality of grape tomato, spinach and cantaloupe.
[So] Source:Int J Food Microbiol;249:53-60, 2017 May 16.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was to investigate the efficacy of aerosolized hydrogen peroxide in inactivating bacteria and maintaining quality of grape tomatoes, baby spinach leaves and cantaloupes. Stem scars and smooth surfaces of tomatoes, spinach leaves, and cantaloupe rinds, inoculated with Escherichia coli O157:H7, Salmonella Typhimurium and Listeria innocua, were treated for 45s followed by additional 30min dwell time with hydrogen peroxide (7.8%) aerosols activated by atmospheric cold plasma. Non-inoculated samples were used to study the effects on quality and native microflora populations. Results showed that two ranges of hydrogen peroxide droplets with mean diameters of 40nm and 3.0µm were introduced into the treatment chamber. The aerosolized hydrogen peroxide treatment reduced S. Typhimurium populations by 5.0logCFU/piece, and E. coli O157:H7 and L. innocua populations from initial levels of 2.9 and 6.3logCFU/piece, respectively, to non-detectable levels (detection limit 0.6logCFU/piece) on the smooth surface of tomatoes. However, on the stem scar area of tomatoes, the reductions of E. coli O157:H7, S. Typhimurium, and L. innocua were only 1.0, 1.3, and 1.3 log, respectively. On the cantaloupe rind, the treatment reduced populations of E. coli O157:H7, S. Typhimurium and L. innocua by 4.9, 1.3, and 3.0logCFU/piece, respectively. Under the same conditions, reductions achieved on spinach leaves were 1.5, 4.2 and 4.0 log for E. coli O157:H7, S. Typhimurium and L. innocua, respectively. The treatments also significantly reduced native aerobic plate count, and yeasts and mold count of tomato fruits and spinach leaves. Furthermore, firmness and color of the samples were not significantly affected by the aerosolized hydrogen peroxide. Overall, our results showed that the efficacy of aerosolized hydrogen peroxide depended on type of inoculated bacteria, location of bacteria and type of produce items, and aerosolized hydrogen peroxide could potentially be used to sanitize fresh fruits and vegetables.
[Mh] Termos MeSH primário: Cucumis melo/microbiologia
Escherichia coli O157/efeitos dos fármacos
Conservação de Alimentos/métodos
Peróxido de Hidrogênio/farmacologia
Listeria/efeitos dos fármacos
Lycopersicon esculentum/microbiologia
Salmonella typhimurium/efeitos dos fármacos
Spinacia oleracea/microbiologia
[Mh] Termos MeSH secundário: Aerossóis
Contagem de Colônia Microbiana
Microbiologia de Alimentos/métodos
Seres Humanos
Peróxido de Hidrogênio/química
Gases em Plasma/química
Verduras/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Plasma Gases); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE


  9 / 1845 MEDLINE  
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[PMID]:28289084
[Au] Autor:Mandali S; Gupta K; Dawson AR; Van Duyne GD; Johnson RC
[Ad] Endereço:Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA.
[Ti] Título:Control of Recombination Directionality by the Listeria Phage A118 Protein Gp44 and the Coiled-Coil Motif of Its Serine Integrase.
[So] Source:J Bacteriol;199(11), 2017 Jun 01.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The serine integrase of phage A118 catalyzes integrative recombination between on the phage and a specific locus on the chromosome of , but it is unable to promote excisive recombination between the hybrid and sites found on the integrated prophage without assistance by a recombination directionality factor (RDF). We have identified and characterized the phage-encoded RDF Gp44, which activates the A118 integrase for excision and inhibits integration. Gp44 binds to the C-terminal DNA binding domain of integrase, and we have localized the primary binding site to be within the mobile coiled-coil (CC) motif but distinct from the distal tip of the CC that is required for recombination. This interaction is sufficient to inhibit integration, but a second interaction involving the N-terminal end of Gp44 is also required to activate excision. We provide evidence that these two contacts modulate the trajectory of the CC motifs as they extend out from the integrase core in a manner dependent upon the identities of the four sites. Our results support a model whereby Gp44 shapes the Int-bound complexes to control which sites can synapse and recombine. Serine integrases mediate directional recombination between bacteriophage and bacterial chromosomes. These highly regulated site-specific recombination reactions are integral to the life cycle of temperate phage and, in the case of lysogenized by A118 family phage, are an essential virulence determinant. Serine integrases are also utilized as tools for genetic engineering and synthetic biology because of their exquisite unidirectional control of the DNA exchange reaction. Here, we identify and characterize the recombination directionality factor (RDF) that activates excision and inhibits integration reactions by the phage A118 integrase. We provide evidence that the A118 RDF binds to and modulates the trajectory of the long coiled-coil motif that extends from the large carboxyl-terminal DNA binding domain and is postulated to control the early steps of recombination site synapsis.
[Mh] Termos MeSH primário: Bacteriófagos/enzimologia
Bacteriófagos/genética
Integrases/química
Integrases/metabolismo
Listeria/virologia
Recombinação Genética
Proteínas Virais/química
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Bacteriófagos/química
Bacteriófagos/fisiologia
Regulação Viral da Expressão Gênica
Integrases/genética
Domínios Proteicos
Serina/metabolismo
Proteínas Virais/genética
Integração Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins); 452VLY9402 (Serine); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE


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[PMID]:28216007
[Au] Autor:Yildirim Y; Ertas Onmaz N; Gonulalan Z; Al S; Yildirim A; Karadal F; Hizlisoy H; Pamuk S
[Ad] Endereço:University of Erciyes, Veterinary Faculty, Department of Food Hygiene and Technology, 38039, Kayseri, Turkey. Electronic address: yyildirim@erciyes.edu.tr.
[Ti] Título:Microbiological quality of pastrami and associated surfaces at the point of sale in Kayseri, Turkey.
[So] Source:Public Health;146:152-158, 2017 May.
[Is] ISSN:1476-5616
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The aim of this study is to trace the possible relations between the hygienic status of slicing utensils and the microbiological quality of pastrami. STUDY DESIGN: A total of 75 pastrami retail markets were visited in Kayseri, Turkey, where the pastrami (a ready-to-eat meat product) is commonly produced and consumed. Sliced pastrami, the cutting board and knife surface swabs were collected from each pastrami retail point to trace possible sources of contamination. METHODS: Samples were analysed for the presence of total viable counts (TVC), total coliforms, Escherichia coli, members of Enterobacteriaceae, Staphylococcus aureus and Listeria spp. In addition, pastrami samples were analysed for sulphite-reducing Clostridium spp. and Toxoplasma gondii. RESULTS: When compared with the target values of related literatures, a total of 6 (8%) pastrami samples were found unsatisfactory as a result of TVC (5.3%), Enterobacteriaceae (5.3%), E. coli (2.6%), S. aureus (2.6%), Listeria spp. (2.6%) and Listeria monocytogenes (1.3%) contaminations. No T. gondii positivity was observed among the pastrami samples. None of the cutting board and knife surface swabs were found to harbour TVC level >10 cfu/cm , E. coli and L. monocytogenes. For the total coliforms, 7 (9.3%) and 5 (6.6%) of cutting board and knife surface swabs were found to exceed the target value (<2.5 cfu/cm ), respectively. No statistically significant correlation was detected between the organisms on pastrami and slicing utensils indicating that pastrami were not cross-contaminated by the contact surfaces. CONCLUSION: More emphasis needs to be placed for training of food handlers and to apply good hygienic practices at the point of pastrami sale. The conditions at retail points must be monitored and inspections should be tightened to protect public health.
[Mh] Termos MeSH primário: Comércio
Microbiologia de Alimentos/estatística & dados numéricos
Qualidade dos Alimentos
Produtos da Carne/microbiologia
[Mh] Termos MeSH secundário: Contagem de Colônia Microbiana/estatística & dados numéricos
Enterobacteriaceae/isolamento & purificação
Escherichia coli/isolamento & purificação
Contaminação de Alimentos/análise
Manipulação de Alimentos/normas
Seres Humanos
Higiene/normas
Listeria/isolamento & purificação
Listeria monocytogenes/isolamento & purificação
Staphylococcus aureus/isolamento & purificação
Turquia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE



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