Base de dados : MEDLINE
Pesquisa : B03.353.500.700 [Categoria DeCS]
Referências encontradas : 34 [refinar]
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[PMID]:28901904
[Au] Autor:Lin P; Yan ZF; Li CT; Kook M; Wang QJ; Yi TH
[Ad] Endereço:1​Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
[Ti] Título:Chryseomicrobium deserti sp. nov., isolated from desert soil in South Korea.
[So] Source:Int J Syst Evol Microbiol;67(10):4126-4131, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain positive, aerobic, non-motile, rod-shaped bacterium (THG-T1.18 ) was isolated from desert soil. Growth occurred at 20-35 °C (optimum 28-30 °C), at pH 5-7 (optimum 7) and at 0-4 % NaCl (optimum 0-1 %). Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T1.18 were identified as Chryseomicrobium amylolyticum DSM 23442 (96.6 %), Chryseomicrobium imtechense JCM 16573 (96.3 %) and Chryseomicrobium aureum KACC 17219 (96.1 %). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and one unidentified glycolipid. The quinone system was composed of MK-7, MK-8 and MK-6. The major fatty acids were iso C15 : 0 and anteiso C15 : 0. The type of peptidoglycan was A4ß, containing of l-Orn-D-Glu. The DNA G+C content of strain THG-T1.18 was 50.4 mol%. DNA-DNA hybridization values between strain THG-T1.18 and C. amylolyticum DSM 23442 , C. imtechense JCM 16573 , C. aureum KACC 17219 were 24.7 % (20.1 % reciprocal analysis), 19.5 % (16.1 %) and 10.4 % (6.7 %) respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T1.18 represents a novel species of the genus Chryseomicrobium, for which the name Chryseomicrobium deserti sp. nov. is proposed. The type strain is THG-T1.18 (=KACC 18929 =CCTCC AB 2016179 ).
[Mh] Termos MeSH primário: Clima Desértico
Filogenia
Planococáceas/classificação
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
Planococáceas/genética
Planococáceas/isolamento & purificação
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002264


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[PMID]:28281198
[Au] Autor:Lee CW; Park SH; Lee SG; Shin SC; Han SJ; Kim HW; Park HH; Kim S; Kim HJ; Park H; Park H; Lee JH
[Ad] Endereço:Unit of Polar Genomics, Korea Polar Research Institute, Incheon, 21990, Republic of Korea.
[Ti] Título:Crystal structure of the inactive state of the receiver domain of Spo0A from Paenisporosarcina sp. TG-14, a psychrophilic bacterium isolated from an Antarctic glacier.
[So] Source:J Microbiol;55(6):464-474, 2017 Jun.
[Is] ISSN:1976-3794
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:The two-component phosphorelay system is the most prevalent mechanism for sensing and transducing environmental signals in bacteria. Spore formation, which relies on the two-component phosphorelay system, enables the long-term survival of the glacial bacterium Paenisporosarcina sp. TG-14 in the extreme cold environment. Spo0A is a key response regulator of the phosphorelay system in the early stage of spore formation. The protein is composed of a regulatory N-terminal phospho-receiver domain and a DNA-binding C-terminal activator domain. We solved the three-dimensional structure of the unphosphorylated (inactive) form of the receiver domain of Spo0A (PaSpo0A-R) from Paenisporosarcina sp. TG-14. A structural comparison with phosphorylated (active form) Spo0A from Bacillus stearothermophilus (BsSpo0A) showed minor notable differences. A molecular dynamics study of a model of the active form and the crystal structures revealed significant differences in the α4 helix and the preceding loop region where phosphorylation occurs. Although an oligomerization study of PaSpo0A-R by analytical ultracentrifugation (AUC) has shown that the protein is in a monomeric state in solution, both crosslinking and crystal-packing analyses indicate the possibility of weak dimer formation by a previously undocumented mechanism. Collectively, these observations provide insight into the mechanism of phosphorylation-dependent activation unique to Spo0A.
[Mh] Termos MeSH primário: Proteínas de Ligação a DNA/genética
Camada de Gelo/microbiologia
Planococáceas/genética
Domínios Proteicos/genética
Esporos Bacterianos/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Regiões Antárticas
Proteínas de Bactérias/genética
Temperatura Baixa
Cristalografia por Raios X
Simulação de Dinâmica Molecular
Fosforilação
Planococáceas/isolamento & purificação
Estrutura Secundária de Proteína
Transdução de Sinais/genética
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA-Binding Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1007/s12275-017-6599-9


  3 / 34 MEDLINE  
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[PMID]:27669827
[Au] Autor:Markande AR; Nerurkar AS
[Ad] Endereço:a Department of Microbiology and Biotechnology Centre, Faculty of Science , The Maharaja Sayajirao University of Baroda , Vadodara , India.
[Ti] Título:Bioemulsifier (BE-AM1) produced by Solibacillus silvestris AM1 is a functional amyloid that modulates bacterial cell-surface properties.
[So] Source:Biofouling;32(10):1153-62, 2016 Nov.
[Is] ISSN:1029-2454
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel estuarine bacterial strain, Solibacillus silvestris AM1, produces an extracellular, thermostable and fibrous, glycoprotein bioemulsifier (BE-AM1). The amyloid nature of the bioemulsifier (BE-AM1) was confirmed by biophysical techniques (Congo red based polarization microscopy, ThioflavinS based fluorescent microscopy, fibrous arrangement in transmission electron microscopy and secondary structure measurement by FTIR and CD spectrum analysis). Cell-bound BE-AM1 production by S. silvestris AM1 during the mid-logarithmic phase of growth coincided with a decrease in cell surface hydrophobicity, and an increase in cell autoaggregation and biofilm formation. It was observed that the total interfacial interaction energy ([Formula: see text]) for the surface of the bioemulsifier producing S. silvestris AM1 and different derivatized surfaces of polystyrene (silanized and sulfonated) was found to support biofilm formation. This study has revealed that the BE-AM1, a bacterial bioemulsifier, is a functional amyloid and has a role in biofilm formation and cell surface modulation in S. silvestris AM1.
[Mh] Termos MeSH primário: Amiloide/metabolismo
Aderência Bacteriana/fisiologia
Biofilmes/crescimento & desenvolvimento
Planococáceas/fisiologia
[Mh] Termos MeSH secundário: Amiloide/química
Dicroísmo Circular
Interações Hidrofóbicas e Hidrofílicas
Microscopia Eletrônica de Transmissão
Planococáceas/metabolismo
Poliestirenos/química
Conformação Proteica
Espectroscopia de Infravermelho com Transformada de Fourier
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amyloid); 0 (Polystyrenes)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160928
[St] Status:MEDLINE
[do] DOI:10.1080/08927014.2016.1232716


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[PMID]:27667642
[Au] Autor:Srinivas A; Divyasree B; Sasikala C; Tushar L; Bharti D; Ramana CV
[Ad] Endereço:1​Bacterial Discovery Laboratory, Center for Environment, Institute of Science and Technology, J.N.T. University Hyderabad, Kukatpally, Hyderabad 500085, India.
[Ti] Título:Description of Jeotgalibacillus alkaliphilus sp. nov., isolated from a solar salt pan, and Jeotgalibacillus terrae sp. nov., a name to replace 'Jeotgalibacillus soli' Chen et al. 2010.
[So] Source:Int J Syst Evol Microbiol;66(12):5167-5172, 2016 Dec.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-positive, non-motile, rod-shaped bacterium (strain JC303T) isolated from a salt pan was identified based on 16S rRNA gene sequence analysis as a member of the genus Jeotgalibacillus. It was related most closely to Jeotgalibacillus salarius ASL-1T (99.1 % similarity), Jeotgalibacillusalimentarius YKJ-13T (97.9 %), Jeotgalibacillussoli JSM 081008 (97.9 %), Jeotgalibacillusmalaysiensis D5T (97.8 %), Jeotgalibacillusmarinus DSM 1297T (96.3 %), Jeotgalibacilluscampisalis SF-57T (96.1 %) and J. soli P9T (94.9 %). Genomic relatedness based on DNA-DNA hybridization of strain JC303T with the type strains of the closest related species was less than 40 %. Diphosphatidylglycerol, three aminophospholipids, an unidentified aminoglycolipid, two unidentified phospholipids and an unidentified lipid were the polar lipids of strain JC303T. Major (>10 %) fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C14 : 0. Cell-wall amino acids contained peptidoglycan with l-lysine as the diagnostic diamino acid. Strain JC303T contained MK-7 as the predominant (96 %) menaquinone with the presence of a significant amount (4 %) of MK-8. The DNA G+C content was 43 mol%. On the basis of morphological, physiological, genotypic, phylogenetic and chemotaxonomic analyses, strain JC303T is considered to represent a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus alkaliphilus sp. nov. is proposed. The type strain is JC303T (=KCTC 33662T=LMG 28756T). In addition, we propose to rename J. soli (Chen et al., 2010), an illegitimate homonym of the validly published name Jeotgalibacillus soli(Cunha et al., 2012) as Jeotgalibacillus terrae sp. nov. with type strain JSM 081008T (=DSM 22174T=KCTC 13528T).
[Mh] Termos MeSH primário: Filogenia
Planococáceas/classificação
Cloreto de Sódio
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Índia
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfolipídeos/química
Planococáceas/genética
Planococáceas/isolamento & purificação
RNA Ribossômico 16S/genética
Salinidade
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 451W47IQ8X (Sodium Chloride); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160927
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001491


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[PMID]:27373687
[Au] Autor:Pindi PK; Ashwitha K; Rani AS
[Ad] Endereço:1​Department of Microbiology, Palamuru University, Mahabubnagar-509 001, T.S, India.
[Ti] Título:Chryseomicrobium palamuruense sp. nov., a haloalkalitolerant bacterium isolated from a sediment sample.
[So] Source:Int J Syst Evol Microbiol;66(9):3731-3736, 2016 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel Gram-stain-positive, rod shaped, motile bacterium, designated strain PU1T, was isolated from a sediment sample collected from a drainage near hostel of Palamuru University, Mahabubnagar district, T.S, India (16°43'23″N 77°58'49″E). Cells of strain PU1T are positive for catalase, oxidase, phosphatase, lipase and urease, and negative for gelatinase, amylase, protease, cellulase, lysine decarboxylase and ornithine decarboxylase. The fatty acids were dominated by saturated fatty acids (82.7 %), with a high abundance of iso-C15 : 0 (48.8 %), anteiso-C15 : 0 (7.3 %), iso-C16 : 0 (11.9 %), C16 : 1ω7c alcohol (11.8 %) and iso-C17 : 0 (5.3 %). Strain PU1T contained MK-8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine make up the phospholipid composition. The cell-wall peptidoglycan contains meso-diaminopimelic acid as the diamino acid and cell-wall sugars are d-glucose and d-galactose. 16S rRNA gene sequence analysis indicated Chryseomicrobium imtechense and Chryseomicrobiumamylolyticum, members of family Planococcaceae within the phylum Firmicutes, are the closest related species with 16S rRNA gene sequence similarities of 99 %. Other members of the family Planococcaceae had sequence similarities of 99 %, and DNA-DNA relatedness values between strain PU1T and Chryseomicrobium imtechense MW 10T, Chryseomicrobiumamylolyticum JC16T were 38 and 32 % respectively. The G+C content of DNA of strain PU1T is 48.5 mol%. Based on the above-mentioned phenotypic and phylogenetic characteristics, strain PU1T represents a novel species of the genus Chryseomicrobium for which the name Chryseomicrobiumpalamuruense sp. nov. is proposed. The type strain is PU1T(=CCUG 59101T=JCM 16712T=KCTC 13722T=NBRC106750T).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Filogenia
Planococáceas/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Índia
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfolipídeos/química
Planococáceas/genética
Planococáceas/isolamento & purificação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-38-6 (vitamin MK 8); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001256


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[PMID]:26907585
[Au] Autor:Mual P; Singh NK; Verma A; Schumann P; Krishnamurthi S; Dastager S; Mayilraj S
[Ad] Endereço:1Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology,Sector 39A, Chandigarh 160 036,India.
[Ti] Título:Reclassification of Bacillus isronensisShivaji et al. 2009 as Solibacillus isronensis comb. nov. and emended description of genus Solibacillus Krishnamurthi et al. 2009.
[So] Source:Int J Syst Evol Microbiol;66(5):2113-20, 2016 May.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An investigation into the taxonomic position of Bacillus isronensis MTCC 7902T revealed that the strain shares a common phylogenetic lineage with Solibacillus silvestris MTCC 10789T. It displays considerable overlap in phenotypic properties with the genus Solibacillus, including endospore shape and position, oxidase and catalase activities, presence of iso-C15 : 0, C16 : 1ω7c alcohol and iso-C17 : 1ω7c as major cellular fatty acids, A4α-type cell-wall peptidoglycan, polar lipids and menaquinone pattern. These features reinforce the findings of molecular phylogenetic analyses based on 16S rRNA gene, gyrB gene and 16S-23S internal transcribed spacer (ITS) region sequences and, in line with the recommendations of Kämpfer et al. [Int J Syst Evol Microbiol 56 (2006), 781-786], provide justification for the transfer of Bacillus isronensis from the genus Bacillus to Solibacillus as Solibacillus isronensis comb. nov. The type strain is B3W22T ( = MTCC 7902T = DSM 21046T = JCM 13838T). An emended description of the genus Solibacillus is also provided.
[Mh] Termos MeSH primário: Bacillus/classificação
Filogenia
Planococáceas/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
DNA Espaçador Ribossômico/genética
Ácidos Graxos/química
Genes Bacterianos
Lipídeos/química
Peptidoglicano/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Esporos Bacterianos
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal Spacer); 0 (Fatty Acids); 0 (Lipids); 0 (Peptidoglycan); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.000982


  7 / 34 MEDLINE  
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[PMID]:26808870
[Au] Autor:See-Too WS; Convey P; Pearce DA; Lim YL; Ee R; Yin WF; Chan KG
[Ad] Endereço:Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.
[Ti] Título:Complete genome of Planococcus rifietoensis M8(T), a halotolerant and potentially plant growth promoting bacterium.
[So] Source:J Biotechnol;221:114-5, 2016 Mar 10.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Planococcus rifietoensis M8(T) (=DSM 15069(T)=ATCC BAA-790(T)) is a halotolerant bacterium with potential plant growth promoting properties isolated from an algal mat collected from a sulfurous spring in Campania (Italy). This paper presents the first complete genome of P. rifietoensis M8(T). Genes coding for various potentially plant growth promoting properties were identified within its genome.
[Mh] Termos MeSH primário: Genoma Bacteriano
Planococáceas/isolamento & purificação
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/farmacologia
Composição de Bases
Tamanho do Genoma
Itália
Planococáceas/genética
Desenvolvimento Vegetal/efeitos dos fármacos
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160126
[St] Status:MEDLINE


  8 / 34 MEDLINE  
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[PMID]:25999308
[Au] Autor:Yaakop AS; Chan KG; Gan HM; Goh KM
[Ad] Endereço:Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.
[Ti] Título:Draft genome of Jeotgalibacillus campisalis SF-57(T), a moderate halophilic bacterium isolated from marine saltern.
[So] Source:Mar Genomics;23:59-60, 2015 Oct.
[Is] ISSN:1876-7478
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Jeotgalibacillus campisalis SF-57(T) (=KCCM 41644(T), JCM 11810(T)) is a moderate halophilic bacterium isolated from a Korean marine saltern. In this study, we describe the high-quality draft genome of strain SF-57(T), which was assembled into 24 contigs containing 3,650,490bp with a G+C content of 41.06%. Availability of the genome sequence of J. campisalis SF-57(T) will contribute to a better understanding of the genus Jeotgalibacillus.
[Mh] Termos MeSH primário: Genoma Bacteriano
Planococáceas/genética
[Mh] Termos MeSH secundário: DNA Bacteriano/genética
Água do Mar/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150523
[St] Status:MEDLINE


  9 / 34 MEDLINE  
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[PMID]:25980832
[Au] Autor:Whitehead TR; Johnson CN; Patel NB; Cotta MA; Moore ER; Lawson PA
[Ad] Endereço:Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL, 61604, USA, Terry.Whitehead@ars.usda.gov.
[Ti] Título:Savagea faecisuis gen. nov., sp. nov., a tylosin- and tetracycline-resistant bacterium isolated from a swine-manure storage pit.
[So] Source:Antonie Van Leeuwenhoek;108(1):151-61, 2015 Jul.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular methods was performed on three strains of a Gram-stain positive, non-sporeforming, motile aerobic rod-shaped bacterium resistant to tylosin and tetracycline isolated from a swine-manure storage pit. On the basis of 16S rRNA gene sequence analyses, it was confirmed that these isolates are highly related to each other and form a hitherto unknown lineage within the Planococcaceae. In particular, pairwise analysis of the 16S rRNA gene sequence demonstrated that the novel organism is closely related to members of the genus Sporosarcina (92.8-94.5 %), Pyschrobacillus (93.5-93.9 %) and Paenisporosarcina (93.3-94.5 %). The predominant fatty acids were found to consist of iso-C15:0 and iso-C17:1 ω10c and the G+C mol% was determined to be 41.8. Based on biochemical, chemotaxonomic, and phylogenetic evidence, it is proposed that these novel strains be classified as a novel genus and species, Savagea faecisuis gen nov., sp. nov. The type strain is Con12(T) (=CCUG 63563(T) = NRRL B-59945(T) = NBRC 109956(T)).
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Microbiologia Ambiental
Planococáceas/classificação
Planococáceas/isolamento & purificação
Tetraciclina/farmacologia
Tilosina/farmacologia
[Mh] Termos MeSH secundário: Aerobiose
Animais
Composição de Bases
Análise por Conglomerados
Citosol/química
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Ácidos Graxos/análise
Locomoção
Esterco
Dados de Sequência Molecular
Filogenia
Planococáceas/efeitos dos fármacos
Planococáceas/genética
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Fatty Acids); 0 (Manure); 0 (RNA, Ribosomal, 16S); F8VB5M810T (Tetracycline); YEF4JXN031 (Tylosin)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150606
[Lr] Data última revisão:
150606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150519
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-015-0473-8


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[PMID]:25945579
[Au] Autor:Akita H; Seto T; Ohshima T; Sakuraba H
[Ad] Endereço:Applied Molecular Microbiology and Biomass Chemistry, Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
[Ti] Título:Structural insight into the thermostable NADP(+)-dependent meso-diaminopimelate dehydrogenase from Ureibacillus thermosphaericus.
[So] Source:Acta Crystallogr D Biol Crystallogr;71(Pt 5):1136-46, 2015 May.
[Is] ISSN:1399-0047
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Crystal structures of the thermostable meso-diaminopimelate dehydrogenase (DAPDH) from Ureibacillus thermosphaericus were determined for the enzyme in the apo form and in complex with NADP(+) and N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid. The main-chain coordinates of the enzyme showed notable similarity to those of Symbiobacterium thermophilum DAPDH. However, the subunit arrangement of U. thermosphaericus DAPDH (a dimer) was totally different from that of the S. thermophilum enzyme (a hexamer). Structural comparison with the dimeric enzyme from the mesophile Corynebacterium glutamicum revealed that the presence of large numbers of intrasubunit and intersubunit hydrophobic interactions, as well as the extensive formation of intersubunit ion-pair networks, were likely to be the main factors contributing to the higher thermostability of U. thermosphaericus DAPDH. This differs from S. thermophilum DAPDH, within which the unique hexameric assembly is likely to be responsible for its high thermostability. Analysis of the active site of U. thermosphaericus DAPDH revealed the key factors responsible for the marked difference in substrate specificity between DAPDH and the D-amino acid dehydrogenase recently created from DAPDH by introducing five point mutations [Akita et al. (2012). Biotechnol. Lett. 34, 1693-1699; 1701-1702].
[Mh] Termos MeSH primário: Aminoácido Oxirredutases/química
Aminoácido Oxirredutases/metabolismo
NADP/metabolismo
Planococáceas/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Cristalografia por Raios X
Modelos Moleculares
Dados de Sequência Molecular
Conformação Proteica
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
53-59-8 (NADP); EC 1.4.- (Amino Acid Oxidoreductases); EC 1.4.1.16 (diaminopimelate dehydrogenase)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150507
[Lr] Data última revisão:
150507
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150507
[St] Status:MEDLINE
[do] DOI:10.1107/S1399004715003673



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