Base de dados : MEDLINE
Pesquisa : B03.353.500.875 [Categoria DeCS]
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  1 / 22 MEDLINE  
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[PMID]:28841315
[Au] Autor:Teta R; Marteinsson VT; Longeon A; Klonowski AM; Groben R; Bourguet-Kondracki ML; Costantino V; Mangoni A
[Ad] Endereço:The NeaNat Group, Dipartimento di Farmacia, Università degli Studi di Napoli Federico II , Via D. Montesano 49, 80131 Napoli, Italy.
[Ti] Título:Thermoactinoamide A, an Antibiotic Lipophilic Cyclopeptide from the Icelandic Thermophilic Bacterium Thermoactinomyces vulgaris.
[So] Source:J Nat Prod;80(9):2530-2535, 2017 Sep 22.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The thermophilic bacterium Thermoactinomyces vulgaris strain ISCAR 2354, isolated from a coastal hydrothermal vent in Iceland, was shown to contain thermoactinoamide A (1), a new cyclic hexapeptide composed of mixed d and l amino acids, along with five minor analogues (2-6). The structure of 1 was determined by one- and two-dimensional NMR spectroscopy, high-resolution tandem mass spectrometry, and advanced Marfey's analysis of 1 and of the products of its partial hydrolysis. Thermoactinoamide A inhibited the growth of Staphylococcus aureus ATCC 6538 with an MIC value of 35 µM. On the basis of literature data and this work, cyclic hexapeptides with mixed d/l configurations, one aromatic amino acid residue, and a prevalence of lipophilic residues can be seen as a starting point to define a new, easily accessible scaffold in the search for new antibiotic agents.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Peptídeos Cíclicos/isolamento & purificação
Peptídeos Cíclicos/farmacologia
Staphylococcus aureus/efeitos dos fármacos
Thermoactinomyces/química
[Mh] Termos MeSH secundário: Antibacterianos/química
Estrutura Molecular
Peptídeos Cíclicos/química
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Peptides, Cyclic); 0 (thermoactinoamide A)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00560


  2 / 22 MEDLINE  
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[PMID]:27377749
[Au] Autor:Liu F; Zhao ZS; Ren Y; Cheng G; Tang XF; Tang B
[Ad] Endereço:State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
[Ti] Título:Autocatalytic activation of a thermostable glutamyl endopeptidase capable of hydrolyzing proteins at high temperatures.
[So] Source:Appl Microbiol Biotechnol;100(24):10429-10441, 2016 Dec.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Glutamyl endopeptidases (GSEs) specifically hydrolyze peptide bonds formed by α-carboxyl groups of Glu and Asp residues. We cloned the gene for a thermophilic GSE (designated TS-GSE) from Thermoactinomyces sp. CDF. A proform of TS-GSE that contained a 61-amino acid N-terminal propeptide and a 218-amino acid mature domain was produced in Escherichia coli. We found that the proform possessed two processing sites and was capable of autocatalytic activation via multiple pathways. The N-terminal propeptide could be autoprocessed at the Glu -Ser bond to directly generate the mature enzyme. It could also be autoprocessed at the Glu -Lys bond to yield an intermediate, which was then converted into the mature form after removal of the remaining part of the propeptide. The segment surrounding the two processing sites was flexible, which allowed the proform and the intermediate form to be trans-processed into the mature form by either active TS-GSE or heterogeneous proteases. Deletion analysis revealed that the N-terminal propeptide is important for the correct folding and maturation of TS-GSE. The propeptide, even its last 11-amino acid peptide segment, could inhibit the activity of its cognate mature domain. The mature TS-GSE displayed a temperature optimum of 85 °C and retained approximately 90 % of its original activity after incubation at 70 °C for 6 h, representing the most thermostable GSE reported to date. Mutational analysis suggested that the disulfide bonds Cys -Cys and Cys -Cys cumulatively contributed to the thermostability of TS-GSE.
[Mh] Termos MeSH primário: Precursores de Proteínas/química
Precursores de Proteínas/metabolismo
Proteólise
Serina Endopeptidases/química
Serina Endopeptidases/metabolismo
Thermoactinomyces/enzimologia
[Mh] Termos MeSH secundário: Clonagem Molecular
Análise Mutacional de DNA
Estabilidade Enzimática
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Temperatura Alta
Dobramento de Proteína
Processamento de Proteína Pós-Traducional
Deleção de Sequência
Thermoactinomyces/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Precursors); EC 3.4.21.- (Serine Endopeptidases); EC 3.4.21.19 (glutamyl endopeptidase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160706
[St] Status:MEDLINE


  3 / 22 MEDLINE  
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[PMID]:26910402
[Au] Autor:Bell ME; Lasker BA; Klenk HP; Hoyles L; Spröer C; Schumann P; Brown JM
[Ad] Endereço:Centers for Disease Control and Prevention, Building 17, Room 2209, Mailstop D-11, 1600 Clifton Road, Atlanta, GA, 30333, USA. jqv7@cdc.gov.
[Ti] Título:Kroppenstedtia pulmonis sp. nov. and Kroppenstedtia sanguinis sp. nov., isolated from human patients.
[So] Source:Antonie Van Leeuwenhoek;109(5):603-10, 2016 May.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Three human clinical strains (W9323(T), X0209(T) and X0394) isolated from a lung biopsy, blood and cerebral spinal fluid, respectively, were characterised using a polyphasic taxonomic approach. Comparative analysis of the 16S rRNA gene sequences showed the three strains belong to two novel branches within the genus Kroppenstedtia: 16S rRNA gene sequence analysis of W9323(T) showed close sequence similarity to Kroppenstedtia eburnea JFMB-ATE(T) (95.3 %), Kroppenstedtia guangzhouensis GD02(T) (94.7 %) and strain X0209(T) (94.6 %); sequence analysis of strain X0209(T) showed close sequence similarity to K. eburnea JFMB-ATE(T) (96.4 %) and K. guangzhouensis GD02(T) (96.0 %). Strains X0209(T) and X0394 were 99.9 % similar to each other by 16S rRNA gene sequence analysis. The DNA-DNA relatedness was 94.6 %, confirming that X0209(T) and X0394 belong to the same species. Chemotaxonomic data for strains W9323(T) and X0209(T) were consistent with those described for the members of the genus Kroppenstedtia: the peptidoglycan was found to contain LL-diaminopimelic acid; the major cellular fatty acids were identified as iso-C15 and anteiso-C15; and the major menaquinone was identified as MK-7. Differences in endospore morphology, carbon source utilisation profiles, and cell wall sugar patterns of strains W9323(T) and X0209(T), supported by phylogenetic analysis, enabled us to conclude that the strains each represent a new species within the genus Kroppenstedtia, for which the names Kroppenstedtia pulmonis sp. nov. (type strain W9323(T) = DSM 45752(T) = CCUG 68107(T)) and Kroppenstedtia sanguinis sp. nov. (type strain X0209(T) = DSM 45749(T) = CCUG 38657(T)) are proposed.
[Mh] Termos MeSH primário: Infecções por Bactérias Gram-Positivas/microbiologia
Thermoactinomyces/isolamento & purificação
[Mh] Termos MeSH secundário: Adolescente
Idoso
Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
Feminino
Infecções por Bactérias Gram-Positivas/sangue
Infecções por Bactérias Gram-Positivas/líquido cefalorraquidiano
Seres Humanos
Pulmão/microbiologia
Masculino
Meia-Idade
Filogenia
RNA Ribossômico 16S/genética
Esporos Bacterianos/citologia
Thermoactinomyces/classificação
Thermoactinomyces/citologia
Thermoactinomyces/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-016-0663-z


  4 / 22 MEDLINE  
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[PMID]:26678783
[Au] Autor:Mokrane S; Bouras N; Meklat A; Lahoum A; Zitouni A; Verheecke C; Mathieu F; Schumann P; Spröer C; Sabaou N; Klenk HP
[Ad] Endereço:Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria.
[Ti] Título:Thermoactinomyces khenchelensis sp. nov., a filamentous bacterium isolated from soil sediment of a terrestrial hot spring.
[So] Source:Antonie Van Leeuwenhoek;109(2):311-7, 2016 Feb.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel thermophilic filamentous bacterium, designated strain T36(T), was isolated from soil sediment sample from a hot spring source collected in Khenchela province, Algeria. Strain T36(T) was identified as a member of the genus Thermoactinomyces by a polyphasic approach. Strain T36(T) was observed to form white aerial mycelium and non-coloured to pale yellow substrate mycelium, both producing endospores, sessile or borne by short sporophores. The optimum growth temperature and pH were found to be 37-55 °C and 7.0-9.0, respectively and the optimum NaCl concentration for growth was found to be 0-7 % (w/v). The diagnostic diamino acid in the cell wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinone of strain T36(T) was identified as MK-7 (H0). The major fatty acids were found to be iso-C15:0 and iso-C17:0. The phospholipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphoglycolipid. The chemotaxonomic properties of strain T36(T) are consistent with those shared by members of the genus Thermoactinomyces. 16S rRNA gene sequence analysis indicated that the sequence similarities between strain T36(T) and Thermoactinomyces species with validly published names were less than 98 %. Based on the combined genotypic and phenotypic evidence, it is proposed that strain T36(T) should be classified as representative of a novel species, for which the name Thermoactinomyces khenchelensis sp. nov. is proposed. The type strain is T36(T) (=DSM 45951(T) = CECT 8579(T)).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Fontes Termais/microbiologia
Thermoactinomyces/isolamento & purificação
[Mh] Termos MeSH secundário: Argélia
DNA Bacteriano/genética
DNA Ribossômico/genética
Ácidos Graxos/química
Ácidos Graxos/metabolismo
Dados de Sequência Molecular
Filogenia
RNA Ribossômico 16S/genética
Microbiologia do Solo
Thermoactinomyces/classificação
Thermoactinomyces/genética
Thermoactinomyces/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151219
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-015-0634-9


  5 / 22 MEDLINE  
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[PMID]:26621297
[Au] Autor:Alikhan MM; Kraft CS; Shih JA; Lee FE; Auffermann WF; Berkowitz DM
[Ad] Endereço:Departments of Medicine, Emory University, Atlanta, GA.
[Ti] Título:A 68-Year-Old Musician With Cough, Wheezing, and a Lung Mass.
[So] Source:Chest;148(6):e181-e183, 2015 Dec.
[Is] ISSN:1931-3543
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A 68-year-old man was referred to the pulmonary clinic for evaluation of cough and a 5-cm right upper lobe mass. He was in his usual state of health until 1 year prior when he developed intermittent cough, wheezing, and sinus congestion. He denied any sputum production or hemoptysis. He also denied any fevers, chills, or weight loss. He had received various treatments within the prior 6 months, including short courses of oral prednisone, levofloxacin, and bronchodilators, without any relief of his symptoms.
[Mh] Termos MeSH primário: Tosse/diagnóstico
Imunoglobulina E/sangue
Aspergilose Pulmonar Invasiva
Itraconazol/administração & dosagem
Prednisona/administração & dosagem
Sons Respiratórios/diagnóstico
[Mh] Termos MeSH secundário: Idoso
Anti-Inflamatórios/administração & dosagem
Antifúngicos/administração & dosagem
Tosse/etiologia
Monitoramento de Medicamentos/métodos
Seres Humanos
Aspergilose Pulmonar Invasiva/sangue
Aspergilose Pulmonar Invasiva/diagnóstico
Aspergilose Pulmonar Invasiva/tratamento farmacológico
Aspergilose Pulmonar Invasiva/fisiopatologia
Masculino
Sons Respiratórios/etiologia
Avaliação de Sintomas/métodos
Thermoactinomyces/isolamento & purificação
Tomografia Computadorizada por Raios X/métodos
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antifungal Agents); 304NUG5GF4 (Itraconazole); 37341-29-0 (Immunoglobulin E); VB0R961HZT (Prednisone)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:151202
[St] Status:MEDLINE


  6 / 22 MEDLINE  
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[PMID]:25991661
[Au] Autor:Wu H; Liu B; Pan S
[Ad] Endereço:1​Institute of Applied Microbiology, Guangxi University, Nanning, PR China 2​School of life science and technology, Guangxi University, Nanning, PR China.
[Ti] Título:Thermoactinomyces guangxiensis sp. nov., a thermophilic actinomycete isolated from mushroom compost.
[So] Source:Int J Syst Evol Microbiol;65(9):2859-64, 2015 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel thermophilic actinomycete, designated strain CD-1(T), was isolated from mushroom compost in Nanning, Guangxi province, China. The strain grew at 37-55 °C (optimum 45-50 °C), pH 6.0-11.0 (optimum pH 7.0-9.0) and with 0-2.0% NaCl (optimum 0-1.0%), formed well-developed white aerial mycelium and pale-yellow vegetative mycelium, and single endospores (0.8-1.0 µm diameter) were borne on long sporophores (2-3 µm length). The endospores were spherical-polyhedron in shape with smooth surface. Based on its phenotypic and phylogenetic characteristics, strain CD-1(T) is affiliated to the genus Thermoactinomyces. It contained meso-diaminopimelic acid as the diagnostic diamino acid; the whole-cell sugars were ribose and glucose. Major fatty acids were iso-C15 :  0, C16 : 0, anteiso-C15  : 0 and iso-C17  : 0. MK-7 was the predominant menaquinone. The polar phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylethanolamine containing hydroxylated fatty acids, ninhydrin-positive glycophospholipid, an unknown phospholipid and glycolipids. The G+C content of the genomic DNA was 48.8%. 16S rRNA gene sequence analysis showed that the organism was closely related to Lihuaxuella thermophila YIM 77831(T) (95.69% sequence similarity), Thermoactinomyces daqus H-18(T) (95.49%), Laceyella putida KCTC 3666(T) (95.05%), Thermoactinomyces vulgaris KCTC 9076(T) (95.01%) and Thermoactinomyces intermedius JCM 3312(T) (94.55%). Levels of DNA-DNA relatedness between strain CD-1T and Lihuaxuella thermophila JCM 18059(T), Thermoactinomyces daqus DSM 45914(T), Laceyella putida JCM 8091(T), Thermoactinomyces vulgaris JCM 3162(T) and Thermoactinomyces intermedius JCM 3312(T) were low (22.8, 33.3, 24.7, 29.4 and 30.0%, respectively). A battery of phenotypic, genotypic and DNA-DNA relatedness data indicated that strain CD-1T represented a novel species of the genus Thermoactinomyces, for which the name Thermoactinomyces guangxiensis sp. nov. is proposed. The type strain is CD-1(T) ( = ATCC BAA-2630(T) = CGMCC 4.7156(T)).
[Mh] Termos MeSH primário: Agaricales
Filogenia
Microbiologia do Solo
Thermoactinomyces/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Solo
Esporos Bacterianos
Thermoactinomyces/genética
Thermoactinomyces/isolamento & purificação
Vitamina K 2/análogos & derivados
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0 (Soil); 11032-49-8 (Vitamin K 2); 583-93-7 (Diaminopimelic Acid); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150521
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.000342


  7 / 22 MEDLINE  
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[PMID]:25837785
[Au] Autor:Zhou C; Liu Z; Huang ZL; Dong M; Yu XL; Ning P
[Ad] Endereço:Department of Environment Science and Engineering, Kunming University of Science and Technology, Yunnan 650500, China. Electronic address: c-zhou04@mails.tsinghua.edu.cn.
[Ti] Título:A new strategy for co-composting dairy manure with rice straw: Addition of different inocula at three stages of composting.
[So] Source:Waste Manag;40:38-43, 2015 Jun.
[Is] ISSN:1879-2456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In considering the impact of inoculation time and the characteristics of composting material and inoculants on the usefulness of inoculation, a new composting strategy has been proposed and studied, in which three inocula were inoculated at three stages of composting process respectively: inoculum A (Thermoactinomyces sp. GF1 and GF2) was inoculated before fermentation to increase or maintain high temperature of pile, inoculum B (Coprinus cinerea and Coprinus comatus) was inoculated after thermophilic phase to promote degradation of lignin, and inoculum C (Trichoderma harzianum and Rhizopus oryzae) was inoculated after 30-day fermentation to promote degradation of cellulose. The results showed that the inoculations could significantly enhance the temperature of pile and the degradation of lignocelluloses. When inocula A, B, and C were inoculated into pile, temperature increased from 25°C to 65°C, from 33°C to 39°C and from 33°C to 38°C respectively and 35% lignin and 43% cellulose had been degraded in inoculated pile compared to the degradation of 15% lignin and 25% cellulose in control pile. As a result, the C/N ratio dropped more rapidly degraded in the inoculated pile (reached 20 after 33-day fermentation) than that in the control pile (reached 21.7 after 45-day fermentation). In addition, the volume loss in inoculated pile (76.5%) was higher than that in control pile (53.2%). The study, therefore, indicated that inoculating proper microorganisms at appropriate time improved the composting process and our new composting strategy would be propitious to the co-composting dairy manure with rice straw.
[Mh] Termos MeSH primário: Indústria de Laticínios
Esterco/microbiologia
Oryza/química
Eliminação de Resíduos/métodos
Solo
[Mh] Termos MeSH secundário: Animais
Carbono/química
Bovinos
Celulose/química
Ecossistema
Fermentação
Concentração de Íons de Hidrogênio
Lignina/química
Nitrogênio/química
Rhizopus
Temperatura Ambiente
Thermoactinomyces
Trichoderma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Manure); 0 (Soil); 7440-44-0 (Carbon); 9004-34-6 (Cellulose); 9005-53-2 (Lignin); N762921K75 (Nitrogen)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150404
[St] Status:MEDLINE


  8 / 22 MEDLINE  
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[PMID]:25619204
[Au] Autor:Akparov VKh; Timofeev VI; Khaliullin IG; Svedas V; Chestukhina GG; Kuranova IP
[Ad] Endereço:State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
[Ti] Título:Structural insights into the broad substrate specificity of carboxypeptidase T from Thermoactinomyces vulgaris.
[So] Source:FEBS J;282(7):1214-24, 2015 Apr.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The crystal structures of carboxypeptidase T (CpT) complexes with phenylalanine and arginine substrate analogs - benzylsuccinic acid and (2-guanidinoethylmercapto)succinic acid - were determined by the molecular replacement method at resolutions of 1.57 Å and 1.62 Å to clarify the broad substrate specificity profile of the enzyme. The conservative Leu211 and Leu254 residues (also present in both carboxypeptidase A and carboxypeptidase B) were shown to be structural determinants for recognition of hydrophobic substrates, whereas Asp263 was for recognition of positively charged substrates. Mutations of these determinants modify the substrate profile: the CpT variant Leu211Gln acquires carboxypeptidase B-like properties, and the CpT variant Asp263Asn the carboxypeptidase A-like selectivity. The Pro248-Asp258 loop interacting with Leu254 and Tyr255 was shown to be responsible for recognition of the substrate's C-terminal residue. Substrate binding at the S1' subsite leads to the ligand-dependent shift of this loop, and Leu254 side chain movement induces the conformation rearrangement of the Glu277 residue crucial for catalysis. This is a novel insight into the substrate selectivity of metallocarboxypeptidases that demonstrates the importance of interactions between the S1' subsite and the catalytic center.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Carboxipeptidases/química
Thermoactinomyces/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Catálise
Domínio Catalítico
Cristalografia por Raios X
Interações Hidrofóbicas e Hidrofílicas
Modelos Moleculares
Ligação Proteica
Especificidade por Substrato
Succinatos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Succinates); 884-33-3 (benzylsuccinic acid); EC 3.4.- (Carboxypeptidases); EC 3.4.17.18 (CPT protein, Thermoactinomyces vulgaris)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:150407
[Lr] Data última revisão:
150407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150127
[St] Status:MEDLINE
[do] DOI:10.1111/febs.13210


  9 / 22 MEDLINE  
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[PMID]:25412577
[Au] Autor:Wang L; Cheng G; Ren Y; Dai Z; Zhao ZS; Liu F; Li S; Wei Y; Xiong J; Tang XF; Tang B
[Ad] Endereço:State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
[Ti] Título:Degradation of intact chicken feathers by Thermoactinomyces sp. CDF and characterization of its keratinolytic protease.
[So] Source:Appl Microbiol Biotechnol;99(9):3949-59, 2015 May.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Thermoactinomyces is known for its resistance to extreme environmental conditions and its ability to digest a wide range of hard-to-degrade compounds. Here, Thermoactinomyces sp. strain CDF isolated from soil was found to completely degrade intact chicken feathers at 55 °C, with the resulting degradation products sufficient to support growth as the primary source of both carbon and nitrogen. Although feathers were not essential for the expression of keratinase, the use of this substrate led to a further 50-300 % increase in enzyme production level under different nutrition conditions, with extracellular keratinolytic activity reaching its highest level (∼400 U/mL) during the late-log phase. Full degradation of feathers required the presence of living cells, which are thought to supply reducing agents necessary for the cleavage of keratin disulfide bonds. Direct contact between the hyphae and substrate may enhance the reducing power and protease concentrations present in the local microenvironment, thereby facilitating keratin degradation. The gene encoding the major keratinolytic protease (protease C2) of strain CDF was cloned, revealing an amino acid sequence identical to that of subtilisin-like E79 protease from Thermoactinomyces sp. E79, albeit with significant differences in the upstream flanking region. Exogenous expression of protease C2 in Escherichia coli resulted in the production of inclusion bodies with proteolytic activity, which could be solubilized to an alkaline solution to produce mature protease C2. Purified protease C2 was able to efficiently hydrolyze α- and ß-keratins at 60-80 °C and pH 11.0, representing a promising candidate for enzymatic processing of hard-to-degrade proteins such as keratinous wastes.
[Mh] Termos MeSH primário: Plumas/metabolismo
Queratinas/metabolismo
Peptídeo Hidrolases/metabolismo
Thermoactinomyces/enzimologia
[Mh] Termos MeSH secundário: Animais
Carbono/metabolismo
Galinhas
Clonagem Molecular
Estabilidade Enzimática
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Concentração de Íons de Hidrogênio
Corpos de Inclusão
Nitrogênio/metabolismo
Homologia de Sequência de Aminoácidos
Microbiologia do Solo
Temperatura Ambiente
Thermoactinomyces/crescimento & desenvolvimento
Thermoactinomyces/isolamento & purificação
Thermoactinomyces/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
68238-35-7 (Keratins); 7440-44-0 (Carbon); EC 3.4.- (Peptide Hydrolases); EC 3.4.- (keratinase); N762921K75 (Nitrogen)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150416
[Lr] Data última revisão:
150416
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141122
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-014-6207-4


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[PMID]:25200773
[Au] Autor:Buehner KP; Anand S; Djira GD; Garcia A
[Ad] Endereço:Midwest Dairy Foods Research Center, Dairy Science Department, South Dakota State University, Brookings 57007.
[Ti] Título:Prevalence of thermoduric bacteria and spores on 10 Midwest dairy farms.
[So] Source:J Dairy Sci;97(11):6777-84, 2014 Nov.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Thermoduric bacteria (TDB), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. They have the potential to adversely affect the quality and shelf life of products. The objectives of this study were to identify the origin and common species of heat-resistant bacteria occurring during summer and winter on Midwest dairy farms. Bulk tank milk samples were taken from 10 dairy farms located along the South Dakota section of Interstate 29, with herd sizes ranging from 650 to 3,500 lactating dairy cows. Milk samples were profiled for the prevalence of TDB and spore counts (SC). Corn silage samples and swabs of the milking clusters were also taken at the dairies to further profile the potential sources of TDB and SC. The samples were taken 3 times during 2 seasons [winter (January-March) and summer (June-August)] to track seasonal changes in the farm bacterial flora. During winter, the average TDB counts in bulk tank milk were 2.61 log compared with 2.76 log TDB counts in the summer. The SC was 1.08 log in the winter, which was half the 2.06 log SC present in the summer season. Corn silage sampled in winter contained a 7.57 log TDB count compared with an increased 10.77 log TDB count during summer sampling. Concentrations of SC in corn silage reached an average of 6.3 log in winter compared with 11.81 log for summer. The seasonal effect was evident with an increase in summer counts across the board for TDB and SC, both in the feed and bulk tank milk samples. Bacillus licheniformis was the predominant species identified in 62.4% of winter (85 total) and 49.4% of summer (83 total) samples. Bacillus subtilis made up 9.4% of the remaining winter isolates, followed by Bacillus sonorensis at 8.2%. Conversely, B. sonorensis made up 12% of the summer isolates followed by Bacillus pumilus at 10.8%. Bacillus licheniformis is a ubiquitous microbe and was isolated from both TDB and sporeformer categories in all 3 sample types. There were larger increases in SC than TDB, indicating that summer temperatures and conditions may favor proliferation of sporeforming bacteria over that of TDB. In conclusion, samples from bulk tank milk, milking cluster swabs, and corn silage samples at each of the 10 sites indicated that B. licheniformis was the major contaminant species, regardless of season. In this experiment, corn silage was the major environmental source of both TDB and SC with higher concentrations in summer when compared with winter.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Indústria de Laticínios/métodos
Temperatura Alta
Leite/microbiologia
Estações do Ano
Silagem/microbiologia
Esporos Bacterianos/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bacillus/classificação
Bacillus/isolamento & purificação
Bacillus subtilis/isolamento & purificação
Carga Bacteriana
Bovinos
Contagem de Colônia Microbiana
Feminino
Contaminação de Alimentos/análise
Microbiologia de Alimentos
Meio-Oeste dos Estados Unidos
South Dakota
Thermoactinomyces/isolamento & purificação
Zea mays/química
Zea mays/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1507
[Cu] Atualização por classe:141202
[Lr] Data última revisão:
141202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140910
[St] Status:MEDLINE



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