Base de dados : MEDLINE
Pesquisa : B03.353.625 [Categoria DeCS]
Referências encontradas : 155 [refinar]
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[PMID]:29235979
[Au] Autor:Ueki A; Goto K; Kaku N; Ueki K
[Ad] Endereço:Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka, Yamagata 997-8555, Japan.
[Ti] Título:Aminipila butyrica gen. nov., sp. nov., a strictly anaerobic, arginine-decomposing bacterium isolated from a methanogenic reactor of cattle waste.
[So] Source:Int J Syst Evol Microbiol;68(1):443-448, 2018 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A strictly anaerobic bacterial strain (FH042 ) was isolated from a methanogenic reactor treating waste from cattle farms. Cells were stained Gram-positive, straight to gently curved rods with polar flagella. The strain was asaccharolytic. The strain fermented amino acids (l-arginine, l-lysine and l-serine) as growth substrates and produced acetate and butyrate. The optimum temperature for growth was 30 °C and the optimum pH was 6.1-6.8. Oxidase, catalase and nitrate-reducing activities were negative. Hydrogen sulfide was produced. The genomic DNA G+C content of strain FH042 was 44.7±0.2 mol%. The major cellular fatty acids were C18 : 1ω9c DMA, C17 : 2/C17 : 1ω9c (as summed feature), C16 : 0 DMA and C14 : 0. The cell-wall peptidoglycan contained meso-diaminopimelic acid as a diagnostic amino acid. The most closely related described species on the basis of 16S rRNA gene sequences was Anaerovorax odorimutans in the family XIII Incertae Sedis in the order Clostridiales of the class Clostridia with sequence similarity of 95.1 %. Based on the distinct differences in phylogenetic and phenotypic characteristics between strain FH042 and related species, Aminipila butyrica gen. nov., sp. nov. is proposed to accommodate the strain. Type strain is FH042 (=JCM 31555 =DSM 103574 ).
[Mh] Termos MeSH primário: Arginina/metabolismo
Reatores Biológicos/microbiologia
Clostridiales/classificação
Esterco/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Bactérias Anaeróbias/classificação
Técnicas de Tipagem Bacteriana
Composição de Bases
Bovinos
Clostridiales/genética
Clostridiales/isolamento & purificação
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Japão
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Manure); 0 (RNA, Ribosomal, 16S); 583-93-7 (Diaminopimelic Acid); 94ZLA3W45F (Arginine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002534


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[PMID]:29185932
[Au] Autor:Granja-Salcedo YT; Duarte Messana J; Carneiro de Souza V; Lino Dias AV; Takeshi Kishi L; Rocha Rebelo L; Teresinha Berchielli T
[Ad] Endereço:1Department of Animal Science,School of Agricultural and Veterinarian Sciences,Universidade Estadual Paulista (UNESP),via de Acesso Professor Paulo Donato Castellane s/n,Jaboticabal,São Paulo,14884-900,Brazil.
[Ti] Título:Effects of partial replacement of maize in the diet with crude glycerin and/or soyabean oil on ruminal fermentation and microbial population in Nellore steers.
[So] Source:Br J Nutr;118(9):651-660, 2017 Nov.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to determine whether a combination of crude glycerin (CG) and soyabean oil (SO) could be used to partially replace maize in the diet of Nellore steers while maintaining optimum feed utilisation. Eight castrated Nellore steers fitted with ruminal and duodenal cannulas were used in a double 4×4 Latin square design balanced for residual effects, in a factorial arrangement (A×B), when factor A corresponded to the provision of SO, and factor B to the provision of CG. Steers feed SO and CG showed similar DM intake, DM, organic matter and neutral-detergent fibre digestibility to that of steers fed diets without oil and without glycerine (P>0·05). Both diets with CG additions reduced the acetate:propionate ratio and increased the proportion of iso-butyrate, butyrate, iso-valerate and valerate (P<0·05). Steers fed diets containing SO had less total N excretion (P<0·001) and showed greater retained N expressed as % N intake (P=0·022). SO and CG diet generated a greater ruminal abundance of Prevotella, Succinivibrio, Ruminococcus, Syntrophococcus and Succiniclasticum. Archaea abundance (P=0·002) and total ciliate protozoa were less in steers fed diets containing SO (P=0·011). CG associated with lipids could be an energy source, which is a useful strategy for the partial replacement of maize in cattle diets, could result in reduced total N excretion and ruminal methanogens without affecting intake and digestibility.
[Mh] Termos MeSH primário: Fenômenos Fisiológicos da Nutrição Animal
Bovinos/fisiologia
Glicerol/administração & dosagem
Rúmen/microbiologia
Óleo de Soja/administração & dosagem
Zea mays
[Mh] Termos MeSH secundário: Ração Animal/análise
Animais
Bovinos/microbiologia
Clostridiales/isolamento & purificação
Clostridiales/metabolismo
Dieta/veterinária
Digestão
Fermentação
Masculino
Prevotella/isolamento & purificação
Prevotella/metabolismo
Rúmen/metabolismo
Ruminococcus/isolamento & purificação
Ruminococcus/metabolismo
Succinivibrionaceae/isolamento & purificação
Succinivibrionaceae/metabolismo
Veillonellaceae/isolamento & purificação
Veillonellaceae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8001-22-7 (Soybean Oil); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517002689


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[PMID]:28905695
[Au] Autor:Ueki A; Goto K; Ohtaki Y; Kaku N; Ueki K
[Ad] Endereço:Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka, Yamagata 997-8555, Japan.
[Ti] Título:Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum.
[So] Source:Int J Syst Evol Microbiol;67(10):4146-4153, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A strictly anaerobic bacterial strain (SH021 ) was isolated from a methanogenic reactor. Cells were Gram-stain-positive, motile, straight or slightly curved rods. The optimum temperature for growth was 35 °C, and the optimum pH was 6.1-7.7. The strain was asaccharolytic and utilized amino acids as growth substrates. The strain produced acetate and propionate from l-alanine and l-serine, and propionate and butyrate from l-threonine. Branched-chain amino acids (l-isoleucine, l-leucine and l-valine) were utilized weakly, and isovalerate or isobutyrate was produced. Strain SH021 utilized pyruvate and lactate, and converted them to acetate and propionate. The genomic DNA G+C content was 38.2 mol%. Compounds related to iso-C15 : 0 were detected as major components in the cellular fatty acids analysis. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. On the basis of 16S rRNA gene sequences, the most closely related known species were Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans in cluster XIVb of the class Clostridia. Based on the phylogenetic and phenotypic data, Anaerotignum aminivorans gen. nov., sp. nov. is proposed to accommodate strain SH021 (=JCM 31556 =DSM 103575 ). For the three related species of the genus Clostridium, Anaerotignum propionicum comb. nov. (type strain DSM 1682 =JCM 1430 =ATCC 25522 =CCUG 9280 =NCIMB 10656 =VPI 5303 ), Anaerotignum neopropionicum comb. nov. (type strain X4 =DSM 3847 =KCTC 15564 ) and Anaerotignum lactatifermentans comb. nov. (type strain G17 =DSM 14214 =LMG 20954 ) are proposed with emended descriptions of these species.
[Mh] Termos MeSH primário: Clostridiales/classificação
Clostridium/classificação
Filogenia
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
Reatores Biológicos/microbiologia
Bovinos
Clostridiales/genética
Clostridiales/isolamento & purificação
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Japão
Peptidoglicano/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Eliminação de Resíduos Líquidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (RNA, Ribosomal, 16S); 0 (Sewage); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002268


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[PMID]:28901896
[Au] Autor:Li Y; Zhang LL; Liu L; Tian YQ; Liu XF; Li WJ; Dai YM
[Ad] Endereço:1​Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Sichuan 610041, PR China.
[Ti] Título:Paludicola psychrotolerans gen. nov., sp. nov., a novel psychrotolerant chitinolytic anaerobe of the family Ruminococcaceae.
[So] Source:Int J Syst Evol Microbiol;67(10):4100-4103, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A psychrotolerant chitinolytic bacterium, designated NC1253 , was isolated from Zoige wetland on the Qinghai-Tibetan Plateau. This strain was a Gram-stain-positive, spore-forming and rod-shaped anaerobe. NC1253 grew at 4-35 °C, at pH 6.0-8.5 and could grow on chitin as the only carbon resource. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain NC1253 represented a novel bacterial genus within the family Ruminococcaceae. Strain NC1253 has less than 91.0 % similarity with other type strains, such as Harryflintia acetispora V20-281a (90.9 %), Clostridium methylpentosum DSM 5476 (90.8 %), Anaerotruncus colihominis DSM 17241 (89.8 %), Eubacterium siraeum DSM 15702 (89.6 %), and Acetanaerobacterium elongatum Z7 (89.6 %). The major components of the cellular fatty acids were iso-C14 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0. The genomic DNA G+C content was 35.4 mol%. Phenotypic, chemotaxonomic and phylogenetic characteristics allowed strain NC1253 to be clearly distinguished from genera in the family Ruminococcaceae. On the basis of polyphasic taxonomic data, the isolate is considered to represent a novel genus and novel species in the family Ruminococcaceae, for which the name Paludicola psychrotolerans gen. nov., sp. nov., is proposed. The type species is NC1253 (DSM 104738 =KCTC 15582 ).
[Mh] Termos MeSH primário: Clostridiales/classificação
Filogenia
Zonas Úmidas
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
Quitina/metabolismo
Clostridiales/genética
Clostridiales/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 1398-61-4 (Chitin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002260


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[PMID]:28892041
[Au] Autor:Knott GJ; East-Seletsky A; Cofsky JC; Holton JM; Charles E; O'Connell MR; Doudna JA
[Ad] Endereço:Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.
[Ti] Título:Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme.
[So] Source:Nat Struct Mol Biol;24(10):825-833, 2017 Oct.
[Is] ISSN:1545-9985
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:CRISPR adaptive immune systems protect bacteria from infections by deploying CRISPR RNA (crRNA)-guided enzymes to recognize and cut foreign nucleic acids. Type VI-A CRISPR-Cas systems include the Cas13a enzyme, an RNA-activated RNase capable of crRNA processing and single-stranded RNA degradation upon target-transcript binding. Here we present the 2.0-Å resolution crystal structure of a crRNA-bound Lachnospiraceae bacterium Cas13a (LbaCas13a), representing a recently discovered Cas13a enzyme subtype. This structure and accompanying biochemical experiments define the Cas13a catalytic residues that are directly responsible for crRNA maturation. In addition, the orientation of the foreign-derived target-RNA-specifying sequence in the protein interior explains the conformational gating of Cas13a nuclease activation. These results describe how Cas13a enzymes generate functional crRNAs and how catalytic activity is blocked before target-RNA recognition, with implications for both bacterial immunity and diagnostic applications.
[Mh] Termos MeSH primário: Sistemas CRISPR-Cas
Clostridiales/enzimologia
Endonucleases/química
Endonucleases/metabolismo
RNA Guia/química
RNA Guia/metabolismo
[Mh] Termos MeSH secundário: Cristalografia por Raios X
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Guide); EC 3.1.- (Endonucleases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE
[do] DOI:10.1038/nsmb.3466


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[PMID]:28781234
[Au] Autor:Yamano T; Zetsche B; Ishitani R; Zhang F; Nishimasu H; Nureki O
[Ad] Endereço:Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
[Ti] Título:Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1.
[So] Source:Mol Cell;67(4):633-645.e3, 2017 Aug 17.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA) and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) have been harnessed for eukaryotic genome editing. Cpf1 requires a specific nucleotide sequence, called a protospacer adjacent motif (PAM), for target recognition. Besides the canonical TTTV PAM, Cpf1 recognizes suboptimal C-containing PAMs. Here, we report four crystal structures of LbCpf1 in complex with the crRNA and its target DNA containing either TTTA, TCTA, TCCA, or CCCA as the PAM. These structures revealed that, depending on the PAM sequences, LbCpf1 undergoes conformational changes to form altered interactions with the PAM-containing DNA duplexes, thereby achieving the relaxed PAM recognition. Collectively, the present structures advance our mechanistic understanding of the PAM-dependent, crRNA-guided DNA cleavage by the Cpf1 family nucleases.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Proteínas Associadas a CRISPR/metabolismo
Sistemas CRISPR-Cas
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
DNA/metabolismo
Endonucleases/metabolismo
Ácidos Nucleicos Heteroduplexes/metabolismo
RNA Guia/metabolismo
[Mh] Termos MeSH secundário: Acidaminococcus/enzimologia
Acidaminococcus/genética
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Sítios de Ligação
Proteínas Associadas a CRISPR/química
Proteínas Associadas a CRISPR/genética
Clostridiales/enzimologia
Clostridiales/genética
Cristalografia por Raios X
DNA/química
DNA/genética
Endonucleases/química
Endonucleases/genética
Escherichia coli/enzimologia
Escherichia coli/genética
Células HEK293
Seres Humanos
Modelos Moleculares
Conformação de Ácido Nucleico
Ácidos Nucleicos Heteroduplexes/química
Ácidos Nucleicos Heteroduplexes/genética
Ligação Proteica
Conformação Proteica
RNA Guia/química
RNA Guia/genética
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (CRISPR-Associated Proteins); 0 (Nucleic Acid Heteroduplexes); 0 (RNA, Guide); 9007-49-2 (DNA); EC 3.1.- (Endonucleases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE


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[PMID]:28632119
[Au] Autor:Zakharyuk A; Kozyreva L; Ariskina E; Troshina O; Kopitsyn D; Shcherbakova V
[Ad] Endereço:1​Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, 142290, Pushchino, Moscow Region, Russia.
[Ti] Título:Alkaliphilus namsaraevii sp. nov., an alkaliphilic iron- and sulfur-reducing bacterium isolated from a steppe soda lake.
[So] Source:Int J Syst Evol Microbiol;67(6):1990-1995, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel alkaliphilic spore-forming bacterium was isolated from the benthic sediments of the highly mineralized steppe Lake Khilganta (Transbaikal Region, Russia). Cells of the strain, designated Ð¥-07-2T, were straight to slightly curved rods, Gram-stain-positive and motile. Strain Ð¥-07-2T grew in the pH range from 7.0 to 10.7 (optimum pH 9.6-10.3). Growth was observed at 25-47 °C (optimum 30 °C) and at an NaCl concentration from 5 to 150 g l-1 with an optimum at 40 g l-1. Strain Ð¥-07-2T was a chemo-organoheterotroph able to reduce amorphous ferric hydroxide, Fe(III) citrate and elemental sulfur in the presence of yeast extract as the electron donor. It used tryptone, peptone and trypticase with Fe(III) citrate as the electron acceptor. The predominant fatty acids in cell walls were C16:1ω8, iso-C15:0, C14 : 0 3-OH and C16 : 0. The DNA G+C content was 32.6 mol%. 16S rRNA gene sequence analysis revealed that strain Ð¥-07-2T was related most closely to members of the genus Alkaliphilus within the family Clostridiaceae. The closest relative was Alkaliphilus peptidifermentans Z-7036T (96.4 % similarity). On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Ð¥-07-2T represents a novel species in the genus Alkaliphilus, for which the name Alkaliphilus namsaraevii sp. nov. is proposed. The type strain is Ð¥-07-2T (=VKM Ð’-2746Т=DSM 26418Т).
[Mh] Termos MeSH primário: Clostridiales/classificação
Lagos/microbiologia
Filogenia
Bactérias Redutoras de Enxofre/classificação
[Mh] Termos MeSH secundário: Álcalis
Técnicas de Tipagem Bacteriana
Composição de Bases
Clostridiales/genética
Clostridiales/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Compostos Férricos/metabolismo
RNA Ribossômico 16S/genética
Federação Russa
Análise de Sequência de DNA
Enxofre
Bactérias Redutoras de Enxofre/genética
Bactérias Redutoras de Enxofre/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Ferric Compounds); 0 (RNA, Ribosomal, 16S); 70FD1KFU70 (Sulfur)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001904


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[PMID]:28628097
[Au] Autor:Zhong G; Wang H; Li Y; Tran MH; Farzan M
[Ad] Endereço:Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, Florida, USA.
[Ti] Título:Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells.
[So] Source:Nat Chem Biol;13(8):839-841, 2017 Aug.
[Is] ISSN:1552-4469
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II-driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.
[Mh] Termos MeSH primário: Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/metabolismo
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas/genética
RNA Mensageiro/genética
Transcrição Genética/genética
[Mh] Termos MeSH secundário: Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/química
Células Cultivadas
Clostridiales/química
Células HEK293
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors); 0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1038/nchembio.2410


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[PMID]:28556772
[Au] Autor:Sakamoto M; Iino T; Ohkuma M
[Ad] Endereço:2​PRIME, Japan Agency for Medical Research and Development (AMED), Tsukuba, Ibaraki 305-0074, Japan 1​Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
[Ti] Título:Faecalimonas umbilicata gen. nov., sp. nov., isolated from human faeces, and reclassification of Eubacterium contortum, Eubacterium fissicatena and Clostridium oroticum as Faecalicatena contorta gen. nov., comb. nov., Faecalicatena fissicatena comb. nov. and Faecalicatena orotica comb. nov.
[So] Source:Int J Syst Evol Microbiol;67(5):1219-1227, 2017 May.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two bacterial strains, designated EGH7T and TSAH33, were isolated from human faeces and characterized by using a polyphasic taxonomic approach that included analysis of morphology, phenotypic and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA and hsp60 gene sequence analyses. The results of 16S rRNA gene sequence analysis indicated that these strains represented members of the family Lachnospiraceae and formed a monophyletic cluster near Eubacterium contortum JCM 6483T (95 % sequence similarity), Ruminococcus gnavus JCM 6515T (95 %), Clostridium oroticum JCM 1429T (95 %), Eubacterium fissicatena JCM 31501T (95 %) and Clostridium nexile JCM 31500T (94 %). The results of a hsp60 gene sequence analysis supported the phylogenetic tree based on the 16S rRNA gene sequence, with a sequence similarity value of between 77.9 and 84.8 % to the five strains listed above. The novel strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-positive cocco-bacilli. The strains formed characteristic umbilicated colonies on EG agar plates. The major cellular fatty acids were C18 : 1ω9c, C16 : 0 and C18 : 1ω9c dimethyl acetal (DMA). EGH7T and TSAH33 have DNA G+C contents of 46.9 and 45.5 mol%, respectively. On the basis of these data, strains EGH7T and TSAH33 represent a novel species of a novel genus, for which the name Faecalimonas umbilicata gen. nov., sp. nov. is proposed. The type strain of F. umbilicata is EGH7T (=JCM 30896T=DSM 103426T).
[Mh] Termos MeSH primário: Clostridiales/classificação
Fezes/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Clostridiales/genética
Clostridiales/isolamento & purificação
Clostridium/classificação
DNA Bacteriano/genética
Eubacterium/classificação
Ácidos Graxos/química
Genes Bacterianos
Seres Humanos
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001790


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[PMID]:28498996
[Au] Autor:Lee YJ; Arguello ES; Jenq RR; Littmann E; Kim GJ; Miller LC; Ling L; Figueroa C; Robilotti E; Perales MA; Barker JN; Giralt S; van den Brink MRM; Pamer EG; Taur Y
[Ad] Endereço:Infectious Diseases Service and.
[Ti] Título:Protective Factors in the Intestinal Microbiome Against Clostridium difficile Infection in Recipients of Allogeneic Hematopoietic Stem Cell Transplantation.
[So] Source:J Infect Dis;215(7):1117-1123, 2017 Apr 01.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Clostridium difficile infection (CDI) is a frequent complication in recipients of allogeneic hematopoietic stem cell transplantation (allo-HSCT), who receive intensive treatments that significantly disrupt the intestinal microbiota. In this study, we examined the microbiota composition of allo-HSCT recipients to identify bacterial colonizers that confer protection against CDI after engraftment. Methods: Feces collected from adult recipients allo-HSCT at engraftment were analyzed; 16S ribosomal RNA genes were sequenced and analyzed from each sample. Bacterial taxa with protective effects against development of CDI were identified by means of linear discriminant analysis effect size analysis and then further assessed with clinical predictors of CDI using survival analysis. Results: A total of 234 allo-HSCT recipients were studied; postengraftment CDI developed in 53 (22.6%). Within the composition of the microbiota, the presence of 3 distinct bacterial taxa was correlated with protection against CDI: Bacteroidetes, Lachnospiraceae, and Ruminococcaceae. Colonization with these groups at engraftment was associated with a 60% lower risk of CDI, independent of clinical factors. Conclusions: Colonization with these 3 bacterial groups is associated with a lower risk of CDI. These groups have been shown to be vital components of the intestinal microbiota. Targeted efforts to maintain them may help minimize the risk of CDI in this at-risk population.
[Mh] Termos MeSH primário: Infecções por Clostridium/microbiologia
Microbioma Gastrointestinal
Transplante de Células-Tronco Hematopoéticas/efeitos adversos
[Mh] Termos MeSH secundário: Adulto
Bacteroidetes/classificação
Bacteroidetes/isolamento & purificação
Clostridiales/classificação
Clostridiales/isolamento & purificação
Clostridium difficile
Fezes/microbiologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Fatores de Proteção
RNA Ribossômico 16S/genética
Transplante Homólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix011



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