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  1 / 10 MEDLINE  
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[PMID]:28100298
[Au] Autor:Cox LM; Sohn J; Tyrrell KL; Citron DM; Lawson PA; Patel NB; Iizumi T; Perez-Perez GI; Goldstein EJC; Blaser MJ
[Ad] Endereço:2​Department of Microbiology, NYU Langone Medical Center, New York, NY 10016, USA 1​Department of Medicine, NYU Langone Medical Center, New York, NY 10016, USA 3​Department of Neurology, Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA
[Ti] Título:Description of two novel members of the family Erysipelotrichaceae: Ileibacterium valens gen. nov., sp. nov. and Dubosiella newyorkensis, gen. nov., sp. nov., from the murine intestine, and emendation to the description of Faecalibaculum rodentium.
[So] Source:Int J Syst Evol Microbiol;67(5):1247-1254, 2017 05.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To better characterize murine intestinal microbiota, a large number (187) of Gram-positive-staining, rod- and coccoid-shaped, and facultatively or strictly anaerobic bacteria were isolated from small and large intestinal contents from mice. Based on 16S rRNA gene sequencing, a total 115 isolates formed three phylogenetically distinct clusters located within the family Erysipelotrichaceae. Group 1, as represented by strain NYU-BL-A3T, was most closely related to Allobaculum stercoricanis, with 16S rRNA gene sequence similarity values of 87.7 %. A second group, represented by NYU-BL-A4T, was most closely related to Faecalibaculum rodentium, with 86.6 % 16S rRNA gene sequence similarity. A third group had a nearly identical 16S rRNA gene sequence (99.9 %) compared with the recently described Faecalibaculum rodentium, also recovered from a laboratory mouse; however, this strain had a few differences in biochemical characteristics, which are detailed in an emended description. The predominant (>10 %) cellular fatty acids of strain NYU-BL-A3T were C16 : 0 and C18 : 0, and those of strain NYU-BL-A4T were C10 : 0, C16 : 0, C18 : 0 and C18 : 1ω9c. The two groups could also be distinguished by multiple biochemical reactions, with the group represented by NYU-BL-A4T being considerably more active. Based on phylogenetic, biochemical and chemotaxonomic criteria, two novel genera are proposed, Ileibacterium valens gen. nov., sp. nov. with NYU-BL-A3T (=ATCC TSD-63T=DSM 103668T) as the type strain and Dubosiella newyorkensis gen. nov., sp. nov. with NYU-BL-A4T (=ATCC TSD-64T=DSM 103457T) as the type strain.
[Mh] Termos MeSH primário: Faecalibacterium/classificação
Intestinos/microbiologia
Camundongos/microbiologia
Filogenia
Tenericutes/classificação
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Tenericutes/genética
Tenericutes/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001793


  2 / 10 MEDLINE  
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[PMID]:27687331
[Au] Autor:Prosberg M; Bendtsen F; Vind I; Petersen AM; Gluud LL
[Ad] Endereço:a Department of Gastroenterology , Hvidovre Hospital, Copenhagen University , Hvidovre , Denmark.
[Ti] Título:The association between the gut microbiota and the inflammatory bowel disease activity: a systematic review and meta-analysis.
[So] Source:Scand J Gastroenterol;51(12):1407-1415, 2016 Dec.
[Is] ISSN:1502-7708
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The pathogenesis of inflammatory bowel diseases (IBD) involves complex interactions between the microbiome and the immune system. We evaluated the association between the gut microbiota and disease activity in IBD patients. METHODS: Systematic review of clinical studies based on a published protocol. Included patients had ulcerative colitis (UC) or Crohn's disease (CD) classified as active or in remission. We selected bacteria assessed in at least three studies identified through electronic and manual searches (November 2015). Bias control was evaluated with the Newcastle Ottawa scale (NOS). Results of random-effects meta-analyses were presented as mean differences (MD). RESULTS: Three prospective and seven cross-sectional studies (NOS score 6-8) were included. Five studies included patients with CD (231 patients) and eight included patients with UC (392 patients). Compared to patients in remission, patients with active IBD had lower abundance of Clostridium coccoides (MD = -0.49, 95% CI: -0.79 to -0.19), Clostridium leptum (MD = -0.44, 95% CI: -0.74 to -0.14), Faecalibacterium prausnitzii (MD = -0.81, 95% CI: -1.23 to -0.39) and Bifidobacterium (MD = -0.37, 95% CI: -0.56 to -0.17). Subgroup analyses showed a difference in all four bacteria between patients with UC classified as active or in remission. Patients with active CD had fewer C. leptum, F. prausnitzii and Bifidobacterium, but not C. coccoides. CONCLUSION: This systematic review suggests that dysbiosis may be involved in the activity of IBD and that there may be differences between patients with CD and UC.
[Mh] Termos MeSH primário: Colite Ulcerativa/microbiologia
Doença de Crohn/microbiologia
Disbiose/fisiopatologia
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Bifidobacterium/isolamento & purificação
Clostridium/isolamento & purificação
Faecalibacterium/isolamento & purificação
Fezes/microbiologia
Seres Humanos
Indução de Remissão
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE


  3 / 10 MEDLINE  
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[PMID]:27618652
[Au] Autor:Fujimura KE; Sitarik AR; Havstad S; Lin DL; Levan S; Fadrosh D; Panzer AR; LaMere B; Rackaityte E; Lukacs NW; Wegienka G; Boushey HA; Ownby DR; Zoratti EM; Levin AM; Johnson CC; Lynch SV
[Ad] Endereço:Division of Gastroenterology, Department of Medicine, University of California, San Francisco, San Francisco, California, USA.
[Ti] Título:Neonatal gut microbiota associates with childhood multisensitized atopy and T cell differentiation.
[So] Source:Nat Med;22(10):1187-1191, 2016 Oct.
[Is] ISSN:1546-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gut microbiota bacterial depletions and altered metabolic activity at 3 months are implicated in childhood atopy and asthma. We hypothesized that compositionally distinct human neonatal gut microbiota (NGM) exist, and are differentially related to relative risk (RR) of childhood atopy and asthma. Using stool samples (n = 298; aged 1-11 months) from a US birth cohort and 16S rRNA sequencing, neonates (median age, 35 d) were divisible into three microbiota composition states (NGM1-3). Each incurred a substantially different RR for multisensitized atopy at age 2 years and doctor-diagnosed asthma at age 4 years. The highest risk group, labeled NGM3, showed lower relative abundance of certain bacteria (for example, Bifidobacterium, Akkermansia and Faecalibacterium), higher relative abundance of particular fungi (Candida and Rhodotorula) and a distinct fecal metabolome enriched for pro-inflammatory metabolites. Ex vivo culture of human adult peripheral T cells with sterile fecal water from NGM3 subjects increased the proportion of CD4 cells producing interleukin (IL)-4 and reduced the relative abundance of CD4 CD25 FOXP3 cells. 12,13-DiHOME, enriched in NGM3 versus lower-risk NGM states, recapitulated the effect of NGM3 fecal water on relative CD4 CD25 FOXP3 cell abundance. These findings suggest that neonatal gut microbiome dysbiosis might promote CD4 T cell dysfunction associated with childhood atopy.
[Mh] Termos MeSH primário: Asma/epidemiologia
Linfócitos T CD4-Positivos/imunologia
Microbioma Gastrointestinal/genética
Hipersensibilidade/epidemiologia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Asma/imunologia
Bifidobacterium/genética
Linfócitos T CD4-Positivos/metabolismo
Candida/genética
Diferenciação Celular/imunologia
Pré-Escolar
Faecalibacterium/genética
Fezes/química
Feminino
Fatores de Transcrição Forkhead/metabolismo
Microbioma Gastrointestinal/imunologia
Seres Humanos
Hipersensibilidade/imunologia
Lactente
Recém-Nascido
Subunidade alfa de Receptor de Interleucina-2/metabolismo
Interleucina-4/imunologia
Masculino
Razão de Chances
Rhodotorula/genética
Análise de Sequência de RNA
Linfócitos T/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (FOXP3 protein, human); 0 (Forkhead Transcription Factors); 0 (IL2RA protein, human); 0 (Interleukin-2 Receptor alpha Subunit); 0 (RNA, Ribosomal, 16S); 207137-56-2 (Interleukin-4)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160913
[St] Status:MEDLINE
[do] DOI:10.1038/nm.4176


  4 / 10 MEDLINE  
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[PMID]:27353369
[Au] Autor:Duan C; Cui Y; Zhao Y; Zhai J; Zhang B; Zhang K; Sun D; Chen H
[Ad] Endereço:Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, PR China. Electronic address: chrduan@cqu.edu.cn.
[Ti] Título:Evaluation of Faecalibacterium 16S rDNA genetic markers for accurate identification of swine faecal waste by quantitative PCR.
[So] Source:J Environ Manage;181:193-200, 2016 Oct 01.
[Is] ISSN:1095-8630
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A genetic marker within the 16S rRNA gene of Faecalibacterium was identified for use in a quantitative PCR (qPCR) assay to detect swine faecal contamination in water. A total of 146,038 bacterial sequences were obtained using 454 pyrosequencing. By comparative bioinformatics analysis of Faecalibacterium sequences with those of numerous swine and other animal species, swine-specific Faecalibacterium 16S rRNA gene sequences were identified and Polymerase Chain Okabe (PCR) primer sets designed and tested against faecal DNA samples from swine and non-swine sources. Two PCR primer sets, PFB-1 and PFB-2, showed the highest specificity to swine faecal waste and had no cross-reaction with other animal samples. PFB-1 and PFB-2 amplified 16S rRNA gene sequences from 50 samples of swine with positive ratios of 86 and 90%, respectively. We compared swine-specific Faecalibacterium qPCR assays for the purpose of quantifying the newly identified markers. The quantification limits (LOQs) of PFB-1 and PFB-2 markers in environmental water were 6.5 and 2.9 copies per 100 ml, respectively. Of the swine-associated assays tested, PFB-2 was more sensitive in detecting the swine faecal waste and quantifying the microbial load. Furthermore, the microbial abundance and diversity of the microbiomes of swine and other animal faeces were estimated using operational taxonomic units (OTUs). The species specificity was demonstrated for the microbial populations present in various animal faeces.
[Mh] Termos MeSH primário: Faecalibacterium/genética
Fezes/microbiologia
Reação em Cadeia da Polimerase/métodos
RNA Ribossômico 16S/genética
Suínos/microbiologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Primers do DNA
DNA Bacteriano/análise
DNA Ribossômico
Cães
Marcadores Genéticos
Seres Humanos
Microbiota/genética
Filogenia
Sensibilidade e Especificidade
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Genetic Markers); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160630
[St] Status:MEDLINE


  5 / 10 MEDLINE  
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[PMID]:27339421
[Au] Autor:Kaakoush NO; Thomas DS; Ruzayqat MM; Lynch D; Leach ST; Lemberg DA; Day AS; Mitchell HM
[Ad] Endereço:1​ School of Medical Sciences, UNSW Australia, Sydney, NSW 2052, Australia.
[Ti] Título:Campylobacter concisus utilizes blood but not short chain fatty acids despite showing associations with Firmicutes taxa.
[So] Source:Microbiology;162(8):1388-97, 2016 Aug.
[Is] ISSN:1465-2080
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Campylobacter concisus is a member of the oral microbiota that has been associated with the development of inflammatory bowel diseases. However, the role of the bacterium in disease aetiology remains poorly understood. Here, we examine optimal conditions for the growth of C. concisus, and the pathogenic potential of this bacterium in human gastrointestinal cells from the upper tract. Further, the presence of C. concisus in the lower tract of Crohn's disease (CD) patients undergoing therapy is observed, and the associations of C. concisus with the abundance of other microbial taxa and compounds they produce are evaluated. C. concisus strains had the ability to tolerate moderate levels of acidity, adhere to and invade esophageal and gastric cells; however, these properties did not correlate with their pathogenic potential in intestinal cells. The presence of the bacterium in the lower gut of CD patients was associated with an increased relative abundance of Faecalibacterium and Lachnospiraceae incertae sedis. Short chain fatty acids that can be produced by these microbial species did not appear to be responsible for this association. However, we identified genetic similarity between C. concisus and Firmicutes, specifically within aspartate and glutamate racemases. The potential pathogenesis of C. concisus in the upper gastrointestinal tract, and the responsiveness of the bacterium to therapy in a subset of CD patients warrant further investigation into whether this bacterium has a causal role in disease or its presence is incidental.
[Mh] Termos MeSH primário: Sangue/microbiologia
Campylobacter/classificação
Campylobacter/patogenicidade
Doença de Crohn/microbiologia
Ácidos Graxos Voláteis/metabolismo
Firmicutes/classificação
Trato Gastrointestinal/microbiologia
[Mh] Termos MeSH secundário: Aderência Bacteriana/fisiologia
Campylobacter/genética
Campylobacter/metabolismo
Infecções por Campylobacter/microbiologia
Células Cultivadas
Clostridiales/isolamento & purificação
Faecalibacterium/isolamento & purificação
Firmicutes/genética
Trato Gastrointestinal/citologia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids, Volatile)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160625
[St] Status:MEDLINE
[do] DOI:10.1099/mic.0.000328


  6 / 10 MEDLINE  
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[PMID]:27160322
[Au] Autor:Hill CJ; Brown JR; Lynch DB; Jeffery IB; Ryan CA; Ross RP; Stanton C; O'Toole PW
[Ad] Endereço:School of Microbiology, University College Cork, Cork, Ireland.
[Ti] Título:Effect of room temperature transport vials on DNA quality and phylogenetic composition of faecal microbiota of elderly adults and infants.
[So] Source:Microbiome;4(1):19, 2016 05 10.
[Is] ISSN:2049-2618
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Alterations in intestinal microbiota have been correlated with a growing number of diseases. Investigating the faecal microbiota is widely used as a non-invasive and ethically simple proxy for intestinal biopsies. There is an urgent need for collection and transport media that would allow faecal sampling at distance from the processing laboratory, obviating the need for same-day DNA extraction recommended by previous studies of freezing and processing methods for stool. We compared the faecal bacterial DNA quality and apparent phylogenetic composition derived using a commercial kit for stool storage and transport (DNA Genotek OMNIgene GUT) with that of freshly extracted samples, 22 from infants and 20 from older adults. RESULTS: Use of the storage vials increased the quality of extracted bacterial DNA by reduction of DNA shearing. When infant and elderly datasets were examined separately, no differences in microbiota composition were observed due to storage. When the two datasets were combined, there was a difference according to a Wilcoxon test in the relative proportions of Faecalibacterium, Sporobacter, Clostridium XVIII, and Clostridium XlVa after 1 week's storage compared to immediately extracted samples. After 2 weeks' storage, Bacteroides abundance was also significantly different, showing an apparent increase from week 1 to week 2. The microbiota composition of infant samples was more affected than that of elderly samples by storage, with significantly higher Spearman distances between paired freshly extracted and stored samples (p < 0.001). When the microbiota profiles were analysed at the operational taxonomic unit (OTU) level, three infant datasets in the study did not cluster together, while only one elderly dataset did not. The lower microbiota diversity of the infant gut microbiota compared to the elderly gut microbiota (p < 0.001) means that any alteration in the infant datasets has a proportionally larger effect. CONCLUSIONS: The commercial storage vials appear to be suitable for high diversity microbiota samples, but may be less appropriate for lower diversity samples. Differences between fresh and stored samples mean that where storage is unavoidable, a consistent storage regime should be used. We would recommend extraction ideally within the first week of storage.
[Mh] Termos MeSH primário: DNA Bacteriano/genética
Microbioma Gastrointestinal/genética
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Bacteroides/genética
Bacteroides/crescimento & desenvolvimento
Clostridium/genética
Clostridium/crescimento & desenvolvimento
Faecalibacterium/genética
Faecalibacterium/crescimento & desenvolvimento
Fezes/microbiologia
Seres Humanos
Lactente
Intestinos/microbiologia
Meia-Idade
Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160511
[St] Status:MEDLINE
[do] DOI:10.1186/s40168-016-0164-3


  7 / 10 MEDLINE  
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[PMID]:27100182
[Au] Autor:Heinritz SN; Weiss E; Eklund M; Aumiller T; Louis S; Rings A; Messner S; Camarinha-Silva A; Seifert J; Bischoff SC; Mosenthin R
[Ad] Endereço:Institute of Animal Science, University of Hohenheim, Stuttgart, Germany.
[Ti] Título:Intestinal Microbiota and Microbial Metabolites Are Changed in a Pig Model Fed a High-Fat/Low-Fiber or a Low-Fat/High-Fiber Diet.
[So] Source:PLoS One;11(4):e0154329, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The intestinal microbiota and its metabolites appear to be an important factor for gastrointestinal function and health. However, research is still needed to further elaborate potential relationships between nutrition, gut microbiota and host's health by means of a suitable animal model. The present study examined the effect of two different diets on microbial composition and activity by using the pig as a model for humans. Eight pigs were equally allotted to two treatments, either fed a low-fat/high-fiber (LF), or a high-fat/low-fiber (HF) diet for 7 weeks. Feces were sampled at day 7 of every experimental week. Diet effects on fecal microbiota were assessed using quantitative real-time PCR, DNA fingerprinting and metaproteomics. Furthermore, fecal short-chain fatty acid (SCFA) profiles and ammonia concentrations were determined. Gene copy numbers of lactobacilli, bifidobacteria (P<0.001) and Faecalibacterium prausnitzii (P<0.05) were higher in the LF pigs, while Enterobacteriaceae were more abundant in the HF pigs (P<0.001). Higher numbers of proteins affiliated to Enterobacteriaceae were also present in the HF samples. Proteins for polysaccharide breakdown did almost exclusively originate from Prevotellaceae. Total and individual fecal SCFA concentrations were higher for pigs of the LF treatment (P<0.05), whereas fecal ammonia concentrations did not differ between treatments (P>0.05). Results provide evidence that beginning from the start of the experiment, the LF diet stimulated beneficial bacteria and SCFA production, especially butyrate (P<0.05), while the HF diet fostered those bacterial groups which have been associated with a negative impact on health conditions. These findings correspond to results in humans and might strengthen the hypothesis that the response of the porcine gut microbiota to a specific dietary modulation is in support of using the pig as suitable animal model for humans to assess diet-gut-microbiota interactions. Data are available via ProteomeXchange with identifier PXD003447.
[Mh] Termos MeSH primário: Dieta com Restrição de Gorduras
Dieta Hiperlipídica
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Animais
Bifidobacterium
Butiratos/química
Impressões Digitais de DNA
DNA Bacteriano/isolamento & purificação
Fibras na Dieta/metabolismo
Faecalibacterium
Ácidos Graxos Voláteis/metabolismo
Fezes
Dosagem de Genes
Lactobacillus
Masculino
Modelos Animais
Oligonucleotídeos/genética
Proteômica
Distribuição Aleatória
Reação em Cadeia da Polimerase em Tempo Real
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Butyrates); 0 (DNA, Bacterial); 0 (Dietary Fiber); 0 (Fatty Acids, Volatile); 0 (Oligonucleotides)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160422
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0154329


  8 / 10 MEDLINE  
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[PMID]:27018325
[Au] Autor:Altamimi M; Abdelhay O; Rastall RA
[Ad] Endereço:Department of Nutrition and Food Technology, An-Najah National University, Nablus, Palestine. Electronic address: m.altamimi@najah.edu.
[Ti] Título:Effect of oligosaccharides on the adhesion of gut bacteria to human HT-29 cells.
[So] Source:Anaerobe;39:136-42, 2016 Jun.
[Is] ISSN:1095-8274
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The influence of five oligosaccharides (cellobiose, stachyose, raffinose, lactulose and chito-oligosaccharides) on the adhesion of eight gut bacteria (Bifidobacterium bifidum ATCC 29521, Bacteroides thetaiotaomicron ATCC 29148D-5, Clostridium leptum ATCC 29065, Blautia coccoides ATCC 29236, Faecalibacterium prausnitzii ATCC 27766, Bacteroides fragilis ATCC 23745, Clostridium difficile ATCC 43255 and Lactobacillus casei ATCC 393) to mucous secreting and non-mucous secreting HT-29 human epithelial cells, was investigated. In pure culture, the bacteria showed variations in their ability to adhere to epithelial cells. The effect of oligosaccharides diminished adhesion and the presence of mucus played a major factor in adhesion, likely due to high adhesiveness to mucins present in the native human mucus layer covering the whole cell surface. However, clostridia displayed almost the same level of adhesion either with or without mucus being present. Bl. coccoides adhesion was decreased by stachyose and cellobiose in non-mucus-secreting cells in pure culture, while in mixed faecal culture cellobiose displayed the highest antiadhesive activity with an overall average of 65% inhibition amongst tested oligomers and lactulose displayed the lowest with an average of 47.4%. Bifidobacteria, Bacteroides, lactobacilli and clostridia were inhibited within the following ranges 47-78%, 32-65%, 11.7-58% and 64-85% respectively. This means that clostridia were the most strongly influenced members of the microflora amongst the bacterial groups tested in mixed culture. In conclusion, introducing oligosaccharides which are candidate prebiotics into pure or mixed cultures has affected bacterial adhesion.
[Mh] Termos MeSH primário: Aderência Bacteriana/efeitos dos fármacos
Microbioma Gastrointestinal/efeitos dos fármacos
Oligossacarídeos/farmacologia
Prebióticos/análise
[Mh] Termos MeSH secundário: Bacteroides/efeitos dos fármacos
Bacteroides/fisiologia
Bacteroides fragilis/efeitos dos fármacos
Bacteroides fragilis/fisiologia
Bifidobacterium/efeitos dos fármacos
Bifidobacterium/fisiologia
Clostridium/efeitos dos fármacos
Clostridium/fisiologia
Faecalibacterium/efeitos dos fármacos
Faecalibacterium/fisiologia
Células HT29
Seres Humanos
Lactobacillus casei/efeitos dos fármacos
Lactobacillus casei/fisiologia
Mucinas/secreção
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mucins); 0 (Oligosaccharides); 0 (Prebiotics)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170116
[Lr] Data última revisão:
170116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160329
[St] Status:MEDLINE


  9 / 10 MEDLINE  
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[PMID]:26921420
[Au] Autor:Tanaka S; Yamamoto K; Yamada K; Furuya K; Uyeno Y
[Ad] Endereço:Academic Assembly (Institute of Agriculture), Shinshu University, Minamiminowa, Nagano, Japan Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan.
[Ti] Título:Relationship of Enhanced Butyrate Production by Colonic Butyrate-Producing Bacteria to Immunomodulatory Effects in Normal Mice Fed an Insoluble Fraction of Brassica rapa L.
[So] Source:Appl Environ Microbiol;82(9):2693-9, 2016 May.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study was performed to determine the effects of feeding a fiber-rich fraction of Brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (SCFA) production in normal mice. The boiled-water-insoluble fraction of Brassica rapa L. (nozawana), which consists mainly of dietary fiber, was chosen as a test material. A total of 31 male C57BL/6J mice were divided into two groups and housed in a specific-pathogen-free facility. The animals were fed either a control diet or the control diet plus the insoluble B. rapa L. fraction for 2 weeks and sacrificed to determine microbiological and SCFA profiles in lower-gut samples and immunological molecules. rRNA-based quantification indicated that the relative population of Bacteroidetes was markedly lower in the colon samples of the insoluble B. rapa L. fraction-fed group than that in the controls. Populations of the Eubacterium rectale group and Faecalibacterium prausnitzii, both of which are representative butyrate-producing bacteria, doubled after 2 weeks of fraction intake, accompanying a marginal increase in the proportion of colonic butyrate. In addition, feeding with the fraction significantly increased levels of the anti-inflammatory cytokine interleukin-10 (IL-10) and tended to increase splenic regulatory T cell numbers but significantly reduced the population of cells expressing activation markers. We demonstrated that inclusion of the boiled-water-insoluble fraction of B. rapa L. can alter the composition of the gut microbiota to decrease the numbers of Bacteroidetes and to increase the numbers of butyrate-producing bacteria, either of which may be involved in the observed shift in the production of splenic IL-10.
[Mh] Termos MeSH primário: Bactérias/metabolismo
Brassica rapa
Ácido Butírico/metabolismo
Colo/imunologia
Colo/microbiologia
Fibras na Dieta/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Bactérias/efeitos dos fármacos
Bactérias/imunologia
Bactérias/isolamento & purificação
Bacteroidetes/efeitos dos fármacos
Bacteroidetes/isolamento & purificação
Bacteroidetes/metabolismo
Colo/efeitos dos fármacos
Suplementos Nutricionais
Eubacterium/efeitos dos fármacos
Eubacterium/isolamento & purificação
Eubacterium/metabolismo
Faecalibacterium/efeitos dos fármacos
Faecalibacterium/isolamento & purificação
Faecalibacterium/metabolismo
Ácidos Graxos Voláteis/biossíntese
Fezes/microbiologia
Interleucina-10/imunologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Linfócitos T/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dietary Fiber); 0 (Fatty Acids, Volatile); 0 (IL10 protein, mouse); 107-92-6 (Butyric Acid); 130068-27-8 (Interleukin-10)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160228
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.03343-15


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[PMID]:26743644
[Au] Autor:Sun D; Duan C; Shang Y; Ma Y; Tan L; Zhai J; Gao X; Guo J; Wang G
[Ad] Endereço:Key Laboratory for Biorheological Science and Technology of Ministry of Education (Chongqing University), State and Local Joint Engineering Laboratory for Vascular Implants (Chongqing), Bioengineering College of Chongqing University, Chongqing University, Chongqing, China.
[Ti] Título:Application of Faecalibacterium 16S rDNA genetic marker for accurate identification of duck faeces.
[So] Source:Environ Sci Pollut Res Int;23(8):7639-47, 2016 Apr.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to judge the legal duty of pollution liabilities by assessing a duck faeces-specific marker, which can exclude distractions of residual bacteria from earlier contamination accidents. With the gene sequencing technology and bioinformatics method, we completed the comparative analysis of Faecalibacterium sequences, which were associated with ducks and other animal species, and found the sequences unique to duck faeces. Polymerase chain reaction (PCR) and agarose gel electrophoresis techniques were used to verify the reliability of both human and duck faeces-specific primers. The duck faeces-specific primers generated an amplicon of 141 bp from 43.3 % of duck faecal samples, 0 % of control samples and 100 % of sewage wastewater samples that contained duck faeces. We present here the initial evidence of Faecalibacterium-based applicability as human faeces-specificity in China. Meanwhile, this study represents the initial report of a Faecalibacterium marker for duck faeces and suggests an independent or supplementary environmental biotechnology of microbial source tracking (MST).
[Mh] Termos MeSH primário: DNA Ribossômico/genética
Patos/microbiologia
Faecalibacterium/genética
Faecalibacterium/isolamento & purificação
Fezes/microbiologia
Marcadores Genéticos/genética
[Mh] Termos MeSH secundário: Animais
China
Seres Humanos
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal); 0 (Genetic Markers); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160109
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-015-6024-z



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