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[PMID]:28646634
[Au] Autor:Vandieken V; Niemann H; Engelen B; Cypionka H
[Ad] Endereço:1​Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, 26129 Oldenburg, Germany.
[Ti] Título:Marinisporobacter balticus gen. nov., sp. nov., Desulfosporosinus nitroreducens sp. nov. and Desulfosporosinus fructosivorans sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea.
[So] Source:Int J Syst Evol Microbiol;67(6):1887-1893, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Four novel Gram-stain-positive, endospore-forming bacteria of the order Clostridiales were isolated from subsurface sediments sampled during International Ocean Discovery Program Expedition 347 to the Baltic Sea. One strain (59.4MT) grew as an obligate heterotroph by aerobic respiration and anaerobically by fermentation. Optimum growth was observed with 0.5 % NaCl at 25 °C and pH 7.0-7.3. Analysis of 16S rRNA gene sequences of 59.4MT revealed Alkaliphilus transvaalensis (92.3 % identity), Candidatus Geosporobacter ferrireducens (92.2 %), Geosporobacter subterraneus (91.9 %) and Alkaliphilus peptidifermentans (91.7 %) to be the closest relatives. On the basis of the results of phenotypic and genotypic analyses, we propose that strain 59.4MT represents a novel species within a novel genus, Marinisporobacter balticus gen. nov., sp. nov., with the type strain 59.4MT (=DSM 102940T=JCM 31103T). Three other strains, 59.4F, 59.4BT and 63.6FT, were affiliated with the genus Desulfosporosinus and grew as strictly anaerobic sulfate reducers. These strains additionally used thiosulfate, elemental sulfur, sulfite and DMSO as electron acceptors and hydrogen as an electron donor. Strains 59.4F and 59.4BT had identical 16S rRNA gene sequences, which were most similar to those of Desulfosporosinus lacus (97.8 %), Desulfosporosinus hippei (97.3 %) and Desulfosporosinus orientis (97.3 %). Strain 63.6FT was closely related to D. lacus (97.7 %), Desulfosporosinus meridiei (96.6 %) and D. hippei (96.5 %). The similarity of 16S rRNA gene sequences of strains 59.4BT and 63.6FT was 96.6 %. We propose the new names Desulfosporosinus nitroreducens sp. nov., incorporating strain 59.4F (=DSM 101562=JCM 31104) and the type strain 59.4BT (=DSM 101608T=JCM 31105T), and Desulfosporosinus fructosivorans sp. nov., with the type strain 63.6FT (=DSM 101609T=JCM 31106T).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Peptococcaceae/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Oxirredução
Peptococcaceae/genética
Peptococcaceae/isolamento & purificação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Bactérias Redutoras de Enxofre/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001883


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[PMID]:28292625
[Au] Autor:Kleindienst S; Higgins SA; Tsementzi D; Chen G; Konstantinidis KT; Mack EE; Löffler FE
[Ad] Endereço:University of Tennessee and Oak Ridge National Laboratory (UT-ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA; Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA; Center for Environmenta
[Ti] Título:'Candidatus Dichloromethanomonas elyunquensis' gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family.
[So] Source:Syst Appl Microbiol;40(3):150-159, 2017 Apr.
[Is] ISSN:1618-0984
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Taxonomic assignments of anaerobic dichloromethane (DCM)-degrading bacteria remain poorly constrained but are important for understanding the microbial diversity of organisms contributing to DCM turnover in environmental systems. We describe the taxonomic classification of a novel DCM degrader in consortium RM obtained from pristine Rio Mameyes sediment. Phylogenetic analysis of full-length 16S rRNA gene sequences demonstrated that the DCM degrader was most closely related to members of the genera Dehalobacter and Syntrophobotulus, but sequence similarities did not exceed 94% and 93%, respectively. Genome-aggregate average amino acid identities against Peptococcaceae members did not exceed 66%, suggesting that the DCM degrader does not affiliate with any described genus. Phylogenetic analysis of conserved single-copy functional genes supported that the DCM degrader represents a novel clade. Growth strictly depended on the presence of DCM, which was consumed at a rate of 160±3µmolL d . The DCM degrader attained 5.25×10 ±1.0×10 cells per µmol DCM consumed. Fluorescence in situ hybridization revealed rod-shaped cells 4±0.8µm long and 0.4±0.1µm wide. Based on the unique phylogenetic, genomic, and physiological characteristics, we propose that the DCM degrader represents a new genus and species, 'Candidatus Dichloromethanomonas elyunquensis'.
[Mh] Termos MeSH primário: Cloreto de Metileno/metabolismo
Peptococcaceae/classificação
Peptococcaceae/metabolismo
[Mh] Termos MeSH secundário: Cromatografia Gasosa
Microbiologia Ambiental
Hibridização in Situ Fluorescente
Cloreto de Metileno/química
Fases de Leitura Aberta
Peptococcaceae/genética
Peptococcaceae/isolamento & purificação
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 588X2YUY0A (Methylene Chloride)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE


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[PMID]:28233989
[Au] Autor:Zhang S; Wondrousch D; Cooper M; Zinder SH; Schüürmann G; Adrian L
[Ad] Endereço:Institute for Organic Chemistry, Technical University Bergakademie Freiberg , Leipziger Straße 29, 09596 Freiberg, Germany.
[Ti] Título:Anaerobic Dehalogenation of Chloroanilines by Dehalococcoides mccartyi Strain CBDB1 and Dehalobacter Strain 14DCB1 via Different Pathways as Related to Molecular Electronic Structure.
[So] Source:Environ Sci Technol;51(7):3714-3724, 2017 Apr 04.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dehalococcoides mccartyi strain CBDB1 and Dehalobacter strain 14DCB1 are organohalide-respiring microbes of the phyla Chloroflexi and Firmicutes, respectively. Here, we report the transformation of chloroanilines by these two bacterial strains via dissimilar dehalogenation pathways and discuss the underlying mechanism with quantum chemically calculated net atomic charges of the substrate Cl, H, and C atoms. Strain CBDB1 preferentially removed Cl doubly flanked by two Cl or by one Cl and NH , whereas strain 14DCB1 preferentially dechlorinated Cl that has an ortho H. For the CBDB1-mediated dechlorination, comparative analysis with Hirshfeld charges shows that the least-negative Cl discriminates active from nonactive substrates in 14 out of 15 cases and may represent the preferred site of primary attack through cob(I)alamin. For the latter trend, three of seven active substrates provide strong evidence, with partial support from three of the remaining four substrates. Regarding strain 14DCB1, the most positive carbon-attached H atom discriminates active from nonactive chloroanilines in again 14 out of 15 cases. Here, regioselectivity is governed for 10 of the 11 active substrates by the most positive H attached to the highest-charge (most positive or least negative) aromatic C carrying the Cl to be removed. These findings suggest the aromatic ring H as primary site of attack through the supernucleophile Co(I), converting an initial H bond to a full electron transfer as start of the reductive dehalogenation. For both mechanisms, one- and two-electron transfer to Cl (strain CBDB1) or H (strain 14DCB1) are compatible with the presently available data. Computational chemistry research into reaction intermediates and pathways may further aid in understanding the bacterial reductive dehalogenation at the molecular level.
[Mh] Termos MeSH primário: Chloroflexi/metabolismo
Halogenação
[Mh] Termos MeSH secundário: Estrutura Molecular
Peptococcaceae
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1021/acs.est.6b05730


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[PMID]:28011598
[Au] Autor:Dong X; Dröge J; von Toerne C; Marozava S; McHardy AC; Meckenstock RU
[Ad] Endereço:Biofilm Centre, University of Duisburg-Essen, Universitätsstrasse 5, 45141 Essen, Germany.
[Ti] Título:Reconstructing metabolic pathways of a member of the genus Pelotomaculum suggesting its potential to oxidize benzene to carbon dioxide with direct reduction of sulfate.
[So] Source:FEMS Microbiol Ecol;93(3), 2017 Mar 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The enrichment culture BPL is able to degrade benzene with sulfate as electron acceptor and is dominated by an organism of the genus Pelotomaculum. Members of Pelotomaculum are usually known to be fermenters, undergoing syntrophy with anaerobic respiring microorganisms or methanogens. By using a metagenomic approach, we reconstructed a high-quality genome (∼2.97 Mbp, 99% completeness) for Pelotomaculum candidate BPL. The proteogenomic data suggested that (1) anaerobic benzene degradation was activated by a yet unknown mechanism for conversion of benzene to benzoyl-CoA; (2) the central benzoyl-CoA degradation pathway involved reductive dearomatization by a class II benzoyl-CoA reductase followed by hydrolytic ring cleavage and modified ß-oxidation; (3) the oxidative acetyl-CoA pathway was utilized for complete oxidation to CO2. Interestingly, the genome of Pelotomaculum candidate BPL has all the genes for a complete sulfate reduction pathway including a similar electron transfer mechanism for dissimilatory sulfate reduction as in other Gram-positive sulfate-reducing bacteria. The proteome analysis revealed that the essential enzymes for sulfate reduction were all formed during growth with benzene. Thus, our data indicated that, besides its potential to anaerobically degrade benzene, Pelotomaculum candidate BPL is the first member of the genus that can perform sulfate reduction.
[Mh] Termos MeSH primário: Benzeno/metabolismo
Dióxido de Carbono/metabolismo
Peptococcaceae/metabolismo
Sulfatos/metabolismo
[Mh] Termos MeSH secundário: Acil Coenzima A/metabolismo
Anaerobiose
Redes e Vias Metabólicas
Oxirredução
Oxirredutases atuantes sobre Doadores de Grupo CH-CH
Proteoma/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acyl Coenzyme A); 0 (Proteome); 0 (Sulfates); 142M471B3J (Carbon Dioxide); 6756-74-7 (benzoyl-coenzyme A); EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors); EC 1.3.99.- (benzoyl-coenzyme A reductase (dearomatizing)); J64922108F (Benzene)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161225
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fiw254


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[PMID]:27909745
[Au] Autor:Lu Y; Ramiro-Garcia J; Vandermeeren P; Herrmann S; Cichocka D; Springael D; Atashgahi S; Smidt H
[Ad] Endereço:Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
[Ti] Título:Dechlorination of three tetrachlorobenzene isomers by contaminated harbor sludge-derived enrichment cultures follows thermodynamically favorable reactions.
[So] Source:Appl Microbiol Biotechnol;101(6):2589-2601, 2017 Mar.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Dechlorination patterns of three tetrachlorobenzene isomers, 1,2,3,4-, 1,2,3,5-, and 1,2,4,5-TeCB, were studied in anoxic microcosms derived from contaminated harbor sludge. The removal of doubly, singly, and un-flanked chlorine atoms was noted in 1,2,3,4- and 1,2,3,5-TeCB fed microcosms, whereas only singly flanked chlorine was removed in 1,2,4,5-TeCB microcosms. The thermodynamically more favorable reactions were selectively followed by the enriched cultures with di- and/or mono-chlorobenzene as the main end products of the reductive dechlorination of all three isomers. Based on quantitative PCR analysis targeting 16S rRNA genes of known organohalide-respiring bacteria, the growth of Dehalococcoides was found to be associated with the reductive dechlorination of all three isomers, while growth of Dehalobacter, another known TeCB dechlorinator, was only observed in one 1,2,3,5-TeCB enriched microcosm among biological triplicates. Numbers of Desulfitobacterium and Geobacter as facultative dechlorinators were rather stable suggesting that they were not (directly) involved in the observed TeCB dechlorination. Bacterial community profiling suggested bacteria belonging to the phylum Bacteroidetes and the order Clostridiales as well as sulfate-reducing members of the class Deltaproteobacteria as putative stimulating guilds that provide electron donor and/or organic cofactors to fastidious dechlorinators. Our results provide a better understanding of thermodynamically preferred TeCB dechlorinating pathways in harbor environments and microbial guilds enriched and active in anoxic TeCB dechlorinating microcosms.
[Mh] Termos MeSH primário: Cloro/metabolismo
Clorobenzenos/metabolismo
DNA Bacteriano/genética
Consórcios Microbianos/genética
Esgotos/microbiologia
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Cloro/isolamento & purificação
Clorobenzenos/isolamento & purificação
Chloroflexi/genética
Chloroflexi/metabolismo
Desulfitobacterium/genética
Desulfitobacterium/metabolismo
Geobacter/genética
Geobacter/metabolismo
Seres Humanos
Peptococcaceae/genética
Peptococcaceae/metabolismo
Esgotos/química
Estereoisomerismo
Termodinâmica
Poluentes Químicos da Água/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorobenzenes); 0 (DNA, Bacterial); 0 (Sewage); 0 (Water Pollutants, Chemical); 4R7X1O2820 (Chlorine); MH0UY3V1KE (1,2,3,4-tetrachlorobenzene)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161203
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-016-8004-8


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[PMID]:27902279
[Au] Autor:Sorokin DY; Chernyh NA
[Ad] Endereço:1​Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Leninskii Avenue 33/2, Moscow 119071, Russia 2​Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
[Ti] Título:Desulfonatronospira sulfatiphila sp. nov., and Desulfitispora elongata sp. nov., two novel haloalkaliphilic sulfidogenic bacteria from soda lakes.
[So] Source:Int J Syst Evol Microbiol;67(2):396-401, 2017 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two novel haloalkaliphilic bacteria with dissimilatory sulfidogenic metabolism were recovered from syntrophic associations obtained from anaerobic sediments of hypersaline soda lakes in Kulunda Steppe (Altai, Russia). Strain ASO3-2T was a member of a sulfidogenic syntrophic association oxidizing acetate at extremely haloalkaline conditions, and was isolated in pure culture using formate as electron donor and sulfate as electron acceptor. It was identified as representing a novel member of the genus Desulfonatronospira within the Deltaproteobacteria. In contrast to the two known species of this genus, the novel isolate was able to grow with formate as electron donor and sulfate, as well as with sulfite, as electron acceptor. Strain Acr1T was a minor component in a soda lake syntrophic association converting benzoate to methane and acetate. It became dominant in a subculture fed with crotonate. While growing on crotonate, strain Acr1T formed unusually long cells filled with polyhydroxyalkanoate-like granules. Its metabolism was limited to fermentation of crotonate and pyruvate and the ability to utilize thiosulfate and sulfur/polysulfide as electron acceptor. Strain Acr1T was identified as representing a novel member of the genus Desulfitispora in the class Clostridia. Both isolates were obligately haloalkaliphilic with extreme salt tolerance. On the basis of phenotypic and phylogenetic analyses, the novel sulfidogenic isolates from soda lakes are proposed to represent two novel species: Desulfonatronospira sulfatiphila sp. nov. (ASO3-2T=DSM 100427=UNIQEM U993T) and Desulfitispora elongata sp. nov. (Acr1T=DSM 29990=UNIQEM U994T).
[Mh] Termos MeSH primário: Deltaproteobacteria/classificação
Lagos/microbiologia
Peptococcaceae/classificação
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Deltaproteobacteria/genética
Deltaproteobacteria/isolamento & purificação
Formiatos/química
Concentração de Íons de Hidrogênio
Peptococcaceae/genética
Peptococcaceae/isolamento & purificação
RNA Ribossômico 16S/genética
Federação Russa
Salinidade
Análise de Sequência de DNA
Sulfatos/química
Sulfitos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Formates); 0 (RNA, Ribosomal, 16S); 0 (Sulfates); 0 (Sulfites); 0YIW783RG1 (formic acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001640


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[PMID]:27898054
[Au] Autor:Wang PH; Tang S; Nemr K; Flick R; Yan J; Mahadevan R; Yakunin AF; Löffler FE; Edwards EA
[Ad] Endereço:Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.
[Ti] Título:Refined experimental annotation reveals conserved corrinoid autotrophy in chloroform-respiring Dehalobacter isolates.
[So] Source:ISME J;11(3):626-640, 2017 03.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two novel chlorinated alkane-respiring Dehalobacter restrictus strains CF and DCA were isolated from the same enrichment culture, ACT-3, and characterized. The closed genomes of these highly similar sister strains were previously assembled from metagenomic sequence data and annotated. The isolation of the strains enabled experimental verification of predicted annotations, particularly focusing on irregularities or predicted gaps in central metabolic pathways and cofactor biosynthesis. Similar to D. restrictus strain PER-K23, strains CF and DCA require arginine, histidine and threonine for growth, although the corresponding biosynthesis pathways are predicted to be functional. Using strain CF to experimentally verify annotations, we determined that the predicted defective serine biosynthesis pathway can be rescued with a promiscuous serine hydroxymethyltransferase. Strain CF grew without added thiamine although the thiamine biosynthesis pathway is predicted to be absent; intracellular thiamine diphosphate, the cofactor of carboxylases in central metabolism, was not detected in cell extracts. Thus, strain CF may use amino acids to replenish central metabolites, portending entangled metabolite exchanges in ACT-3. Consistent with annotation, strain CF possesses a functional corrinoid biosynthesis pathway, demonstrated by increasing corrinoid content during growth and guided cobalamin biosynthesis in corrinoid-free medium. Chloroform toxicity to corrinoid-producing methanogens and acetogens may drive the conservation of corrinoid autotrophy in Dehalobacter strains. Heme detection in strain CF cell extracts suggests the 'archaeal' heme biosynthesis pathway also functions in anaerobic Firmicutes. This study reinforces the importance of incorporating enzyme promiscuity and cofactor availability in genome-scale functional predictions and identifies essential nutrient interdependencies in anaerobic dechlorinating microbial communities.
[Mh] Termos MeSH primário: Peptococcaceae/isolamento & purificação
Peptococcaceae/metabolismo
Microbiologia da Água
[Mh] Termos MeSH secundário: Processos Autotróficos
Vias Biossintéticas
Biotina/biossíntese
Clorofórmio/metabolismo
Corrinoides/biossíntese
Heme/biossíntese
Peptococcaceae/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Corrinoids); 42VZT0U6YR (Heme); 6SO6U10H04 (Biotin); 7V31YC746X (Chloroform)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161130
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2016.158


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[PMID]:27871998
[Au] Autor:Ilhan-Sungur E; Ozuolmez D; Çotuk A; Cansever N; Muyzer G
[Ad] Endereço:Department of Biology, Istanbul University, 34134, Vezneciler, Istanbul, Turkey. Electronic address: esungur@istanbul.edu.tr.
[Ti] Título:Isolation of a sulfide-producing bacterial consortium from cooling-tower water: Evaluation of corrosive effects on galvanized steel.
[So] Source:Anaerobe;43:27-34, 2017 Feb.
[Is] ISSN:1095-8274
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sulfidogenic Clostridia and sulfate reducing bacteria (SRB) often cohabit in nature. The presence of these microorganisms can cause microbially influenced corrosion (MIC) of materials in different ways. To investigate this aspect, bacteria were isolated from cooling tower water and used in corrosion tests of galvanized steel. The identity of the isolates was determined by comparative sequence analysis of PCR-amplified 16S rDNA gene fragments, separated by denaturing gradient gel electrophoresis (DGGE). This analysis showed that, in spite of the isolation process, colonies were not pure and consisted of a mixture of bacteria affiliated with Desulfosporosinus meridiei and Clostridium sp. To evaluate the corrosive effect, galvanized steel coupons were incubated with a mixed culture for 4, 8, 24, 72, 96, 168, 360 and 744 h, along with a control set in sterile culture medium only. The corrosion rate was determined by weight loss, and biofilm formation and corroded surfaces were observed by scanning electron microscopy (SEM). Although the sulfide-producing bacterial consortium led to a slight increase in the corrosion of galvanized steel coupons, when compared to the previous studies it can be said that Clostridium sp. can reduce the corrosive effect of the Desulfosporosinus sp. strain.
[Mh] Termos MeSH primário: Bactérias/classificação
Biofilmes/efeitos dos fármacos
Aço/química
Sulfetos/metabolismo
Microbiologia da Água
[Mh] Termos MeSH secundário: Ar Condicionado
Bactérias/genética
Bactérias/crescimento & desenvolvimento
Bactérias/metabolismo
Carboidratos/análise
Clostridium/genética
Clostridium/metabolismo
Corrosão
DNA Bacteriano/química
DNA Ribossômico/química
Eletroforese em Gel de Gradiente Desnaturante
Desulfovibrio/genética
Desulfovibrio/metabolismo
Microscopia Eletrônica de Varredura
Peptococcaceae/genética
Peptococcaceae/metabolismo
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Alinhamento de Sequência
Zinco/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 0 (Sulfides); 12597-69-2 (Steel); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE


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[PMID]:27766749
[Au] Autor:Ranchou-Peyruse M; Gasc C; Guignard M; Aüllo T; Dequidt D; Peyret P; Ranchou-Peyruse A
[Ad] Endereço:Université de Pau et des Pays de l'Adour, Equipe Environnement et Microbiologie, IPREM-CNRS 5254, F-64013, Pau, France.
[Ti] Título:The sequence capture by hybridization: a new approach for revealing the potential of mono-aromatic hydrocarbons bioattenuation in a deep oligotrophic aquifer.
[So] Source:Microb Biotechnol;10(2):469-479, 2017 Mar.
[Is] ISSN:1751-7915
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The formation water of a deep aquifer (853 m of depth) used for geological storage of natural gas was sampled to assess the mono-aromatic hydrocarbons attenuation potential of the indigenous microbiota. The study of bacterial diversity suggests that Firmicutes and, in particular, sulphate-reducing bacteria (Peptococcaceae) predominate in this microbial community. The capacity of the microbial community to biodegrade toluene and m- and p-xylenes was demonstrated using a culture-based approach after several hundred days of incubation. In order to reveal the potential for biodegradation of these compounds within a shorter time frame, an innovative approach named the solution hybrid selection method, which combines sequence capture by hybridization and next-generation sequencing, was applied to the same original water sample. The bssA and bssA-like genes were investigated as they are considered good biomarkers for the potential of toluene and xylene biodegradation. Unlike a PCR approach which failed to detect these genes directly from formation water, this innovative strategy demonstrated the presence of the bssA and bssA-like genes in this oligotrophic ecosystem, probably harboured by Peptococcaceae. The sequence capture by hybridization shows significant potential to reveal the presence of genes of functional interest which have low-level representation in the biosphere.
[Mh] Termos MeSH primário: Água Subterrânea/microbiologia
Hidrocarbonetos Aromáticos/metabolismo
Metagenômica/métodos
Peptococcaceae/isolamento & purificação
Peptococcaceae/metabolismo
[Mh] Termos MeSH secundário: Biota
Biotransformação
Sequenciamento de Nucleotídeos em Larga Escala
Hibridização de Ácido Nucleico
Peptococcaceae/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrocarbons, Aromatic)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170704
[Lr] Data última revisão:
170704
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161022
[St] Status:MEDLINE
[do] DOI:10.1111/1751-7915.12426


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[PMID]:27450427
[Au] Autor:Lusk BG; Parameswaran P; Popat SC; Rittmann BE; Torres CI
[Ad] Endereço:Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, P.O. Box 875701, Tempe, AZ 85287-5701, United States. Electronic address: Bradley.lusk@asu.edu.
[Ti] Título:The effect of pH and buffer concentration on anode biofilms of Thermincola ferriacetica.
[So] Source:Bioelectrochemistry;112:47-52, 2016 Dec.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We assessed the effects of pH and buffer concentration on current production and growth of biofilms of Thermincola ferriacetica - a thermophilic, Gram-positive, anode-respiring bacterium (ARB) - grown on anodes poised at a potential of -0.06V vs. SHE in microbial electrolysis cells (MECs) at 60°C. T. ferriacetica generated current in the pH range of 5.2 to 8.3 with acetate as the electron donor and 50mM bicarbonate buffer. Maximum current density was reduced by ~80% at pH5.2 and ~14% at 7.0 compared to pH8.3. Increasing bicarbonate buffer concentrations from 10mM to 100mM resulted in an increase in the current density by 40±6%, from 6.8±1.1 to 11.2±2.7Am(-2), supporting that more buffer alleviated pH depression within T. ferriacetica biofilms. Confocal laser scanning microscopy (CLSM) images indicated that higher bicarbonate buffer concentrations resulted in larger live biofilm thicknesses: from 68±20µm at 10mM bicarbonate to >150µm at 100mM, supporting that buffer availability was a strong influence on biofilm thickness. In comparison to mesophilic Geobacter sulfurreducens biofilms, the faster transport rates at higher temperature and the ability to grow at relatively lower pH allowed T. ferriacetica to produce higher current densities with lower buffer concentrations.
[Mh] Termos MeSH primário: Fontes de Energia Bioelétrica/microbiologia
Biofilmes/efeitos dos fármacos
Peptococcaceae/efeitos dos fármacos
Peptococcaceae/fisiologia
[Mh] Termos MeSH secundário: Acetatos/farmacologia
Bicarbonatos/farmacologia
Tampões (Química)
Condutividade Elétrica
Eletrodos
Concentração de Íons de Hidrogênio
Peptococcaceae/metabolismo
Prótons
Cloreto de Sódio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Bicarbonates); 0 (Buffers); 0 (Protons); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE



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