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[PMID]: | 22417294 |
[Au] Autor: | Kumar PS; Mason MR; Brooker MR; O'Brien K |
[Ad] Endereço: | Division of Periodontology, College of Dentistry, The Ohio State University, Columbus, OH, USA. |
[Ti] Título: | Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants. |
[So] Source: | J Clin Periodontol;39(5):425-33, 2012 May. | [Is] ISSN: | 1600-051X |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | AIM: Although it is established that peri-implantitis is a bacterially induced disease, little is known about the bacterial profile of peri-implant communities in health and disease. The purpose of the present investigation was to examine the microbial signatures of the peri-implant microbiome in health and disease. MATERIALS AND METHODS: Subgingival and submucosal plaque samples were collected from forty subjects with periodontitis, peri-implantitis, periodontal and peri-implant health and analysed using 16S pyrosequencing. RESULTS: Peri-implant biofilms demonstrated significantly lower diversity than subgingival biofilms in both health and disease, however, several species, including previously unsuspected and unknown organisms, were unique to this niche. The predominant species in peri-implant communities belonged to the genera Butyrivibrio, Campylobacter, Eubacterium, Prevotella, Selenomonas, Streptococcus, Actinomyces, Leptotrichia, Propionibacterium, Peptococcus, Lactococcus and Treponema. Peri-implant disease was associated with lower levels of Prevotella and Leptotrichia and higher levels of Actinomyces, Peptococcus, Campylobacter, non-mutans Streptococcus, Butyrivibrio and Streptococcus mutans than healthy implants. These communities also demonstrated lower levels of Prevotella, non-mutans Streptococcus, Lactobacillus, Selenomonas, Leptotrichia, Actinomyces and higher levels of Peptococcus, Mycoplasma, Eubacterium, Campylobacter, Butyrivibrio, S. mutans and Treponema when compared to periodontitis-associated biofilms. CONCLUSION: The peri-implant microbiome differs significantly from the periodontal community in both health and disease. Peri-implantitis is a microbially heterogeneous infection with predominantly gram-negative species, and is less complex than periodontitis. |
[Mh] Termos MeSH primário: |
Biofilmes/classificação Implantes Dentários/microbiologia Peri-Implantite/microbiologia
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[Mh] Termos MeSH secundário: |
Actinomyces/classificação Adulto Carga Bacteriana Butyrivibrio/classificação Campylobacter/classificação DNA Bacteriano/genética Placa Dentária/microbiologia Falha de Restauração Dentária Eubacterium/classificação Feminino Seres Humanos Lactobacillus/classificação Lactococcus/classificação Leptotrichia/classificação Masculino Metagenoma/genética Mycoplasma/classificação Peptococcus/classificação Periodontite/microbiologia Prevotella/classificação Propionibacterium/classificação RNA Ribossômico 16S/genética Selenomonas/classificação Análise de Sequência de DNA Streptococcus/classificação Treponema/classificação
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (DNA, Bacterial); 0 (Dental Implants); 0 (RNA, Ribosomal, 16S) |
[Em] Mês de entrada: | 1209 |
[Cu] Atualização por classe: | 161019 |
[Lr] Data última revisão:
| 161019 |
[Sb] Subgrupo de revista: | D; IM |
[Da] Data de entrada para processamento: | 120316 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1111/j.1600-051X.2012.01856.x |
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