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[PMID]:28700642
[Au] Autor:Doyle RM; Harris K; Kamiza S; Harjunmaa U; Ashorn U; Nkhoma M; Dewey KG; Maleta K; Ashorn P; Klein N
[Ad] Endereço:UCL Great Ormond Street Institute of Child Health, University College London, London, United Kingdom.
[Ti] Título:Bacterial communities found in placental tissues are associated with severe chorioamnionitis and adverse birth outcomes.
[So] Source:PLoS One;12(7):e0180167, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Preterm birth is a major cause of neonatal mortality and morbidity worldwide. Bacterial infection and the subsequent inflammatory response are recognised as an important cause of preterm birth. It is hypothesised that these organisms ascend the cervical canal, colonise placental tissues, cause chorioamnionitis and in severe cases infect amniotic fluid and the foetus. However, the presence of bacteria within the intrauterine cavity does not always precede chorioamnionitis or preterm birth. Whereas previous studies observing the types of bacteria present have been limited in size and the specificity of a few predetermined organisms, in this study we characterised bacteria found in placental tissues from a cohort of 1391 women in rural Malawi using 16S ribosomal RNA gene sequencing. We found that specific bacteria found concurrently on placental tissues associate with chorioamnionitis and delivery of a smaller newborn. Severe chorioamnionitis was associated with a distinct difference in community members, a higher bacterial load and lower species richness. Furthermore, Sneathia sanguinengens and Peptostreptococcus anaerobius found in both matched participant vaginal and placental samples were associated with a lower newborn length-for-age Z-score. This is the largest study to date to examine the placental microbiome and its impact of birth outcomes. Our results provide data on the role of the vaginal microbiome as a source of placental infection as well as the possibility of therapeutic interventions against targeted organisms during pregnancy.
[Mh] Termos MeSH primário: Corioamnionite/microbiologia
Microbiota
Placenta/microbiologia
Nascimento Prematuro/microbiologia
[Mh] Termos MeSH secundário: Adulto
Corioamnionite/epidemiologia
Feminino
Seres Humanos
Peptostreptococcus/genética
Peptostreptococcus/isolamento & purificação
Gravidez
Nascimento Prematuro/epidemiologia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180167


  2 / 1314 MEDLINE  
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[PMID]:28126350
[Au] Autor:Tsoi H; Chu ESH; Zhang X; Sheng J; Nakatsu G; Ng SC; Chan AWH; Chan FKL; Sung JJY; Yu J
[Ad] Endereço:Institute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong-Shenzhen Research Institute, Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
[Ti] Título:Peptostreptococcus anaerobius Induces Intracellular Cholesterol Biosynthesis in Colon Cells to Induce Proliferation and Causes Dysplasia in Mice.
[So] Source:Gastroenterology;152(6):1419-1433.e5, 2017 May.
[Is] ISSN:1528-0012
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND & AIMS: Stool samples from patients with colorectal cancer (CRC) have a higher abundance of Peptostreptococcus anaerobius than stool from individuals without CRC, based on metagenome sequencing. We investigated whether P anaerobius contributes to colon tumor formation in mice and its possible mechanisms of carcinogenesis. METHODS: We performed quantitative polymerase chain reaction analyses to measure P anaerobius in 112 stool samples and 255 colon biopsies from patients with CRC or advanced adenoma and from healthy individuals (controls) undergoing colonoscopy examination at hospitals in Hong Kong and Beijing. C57BL/6 mice were given broad-spectrum antibiotics, followed by a single dose of azoxymethane, to induce colon tumor formation. Three days later, mice were given P anaerobius or Esherichia coli MG1655 (control bacteria), via gavage, for 6 weeks. Some mice were also given the nicotinamide adenine dinucleotide phosphate oxidase inhibitor apocynin. Intestine tissues were collected and analyzed histologically. The colon epithelial cell line NCM460 and colon cancer cell lines HT-29 and Caco-2 were exposed to P anaerobius or control bacteria; cells were analyzed by immunoblot, proliferation, and bacterial attachment analyses and compared in gene expression profiling studies. Gene expression was knocked down in these cell lines with small interfering RNAs. RESULTS: P anaerobius was significantly enriched in stool samples from patients with CRC and in biopsies from patients with colorectal adenoma or CRC compared with controls. Mice depleted of bacteria and exposed to azoxymethane and P anaerobius had a higher incidence of intestinal dysplasia (63%) compared with mice not given the bacteria (8.3%; P < .01). P anaerobius mainly colonized the colon compared with the rest of the intestine. Colon cells exposed to P anaerobius had significantly higher levels of proliferation than control cells. We found genes that regulate cholesterol biosynthesis, Toll-like receptor (TLR) signaling, and AMP-activated protein kinase signaling to be significantly up-regulated in cells exposed to P anaerobius. Total cholesterol levels were significantly increased in colon cell lines exposed to P anaerobius via activation of sterol regulatory element-binding protein 2. P anaerobius interacted with TLR2 and TLR4 to increase intracellular levels of reactive oxidative species, which promoted cholesterol synthesis and cell proliferation. Depletion of reactive oxidative species by knockdown of TLR2 or TLR4, or incubation of cells with an antioxidant, prevented P anaerobius from inducing cholesterol biosynthesis and proliferation. CONCLUSIONS: Levels of P anaerobius are increased in human colon tumor tissues and adenomas compared with non-tumor tissues; this bacteria increases colon dysplasia in a mouse model of CRC. P anaerobius interacts with TLR2 and TLR4 on colon cells to increase levels of reactive oxidative species, which promotes cholesterol synthesis and cell proliferation.
[Mh] Termos MeSH primário: Adenoma/metabolismo
Colesterol/biossíntese
Colo/microbiologia
Neoplasias do Colo/metabolismo
Neoplasias do Colo/microbiologia
Infecções por Bactérias Gram-Positivas/metabolismo
Peptostreptococcus
[Mh] Termos MeSH secundário: Proteínas Quinases Ativadas por AMP/genética
Proteínas Quinases Ativadas por AMP/metabolismo
Acetofenonas/farmacologia
Adenoma/microbiologia
Idoso
Animais
Azoximetano
Biópsia
Vias Biossintéticas/genética
Células CACO-2
Estudos de Casos e Controles
Proliferação Celular
Colo/patologia
Neoplasias do Colo/induzido quimicamente
DNA Bacteriano/análise
Inibidores Enzimáticos/farmacologia
Fezes/microbiologia
Expressão Gênica
Infecções por Bactérias Gram-Positivas/complicações
Células HT29
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Meia-Idade
Peptostreptococcus/isolamento & purificação
Espécies Reativas de Oxigênio/metabolismo
Transdução de Sinais/genética
Proteína de Ligação a Elemento Regulador de Esterol 2/metabolismo
Receptor 2 Toll-Like/genética
Receptor 2 Toll-Like/metabolismo
Receptor 4 Toll-Like/genética
Receptor 4 Toll-Like/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetophenones); 0 (DNA, Bacterial); 0 (Enzyme Inhibitors); 0 (Reactive Oxygen Species); 0 (Sterol Regulatory Element Binding Protein 2); 0 (TLR2 protein, human); 0 (TLR4 protein, human); 0 (Toll-Like Receptor 2); 0 (Toll-Like Receptor 4); 97C5T2UQ7J (Cholesterol); B6J7B9UDTR (acetovanillone); EC 2.7.11.31 (AMP-Activated Protein Kinases); MO0N1J0SEN (Azoxymethane)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE


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[PMID]:27797940
[Au] Autor:Wong SH; Kwong TNY; Chow TC; Luk AKC; Dai RZW; Nakatsu G; Lam TYT; Zhang L; Wu JCY; Chan FKL; Ng SSM; Wong MCS; Ng SC; Wu WKK; Yu J; Sung JJY
[Ad] Endereço:State Key Laboratory of Digestive Disease, Department of Medicine and Therapeutics, Institute of Digestive Disease, Hong Kong, Hong Kong.
[Ti] Título:Quantitation of faecal improves faecal immunochemical test in detecting advanced colorectal neoplasia.
[So] Source:Gut;66(8):1441-1448, 2017 Aug.
[Is] ISSN:1468-3288
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: There is a need for an improved biomarker for colorectal cancer (CRC) and advanced adenoma. We evaluated faecal microbial markers for clinical use in detecting CRC and advanced adenoma. DESIGN: We measured relative abundance of ( ), ( ) and ( ) by quantitative PCR in 309 subjects, including 104 patients with CRC, 103 patients with advanced adenoma and 102 controls. We evaluated the diagnostic performance of these biomarkers with respect to faecal immunochemical test (FIT), and validated the results in an independent cohort of 181 subjects. RESULTS: The abundance was higher for all three individual markers in patients with CRC than controls (p<0.001), and for marker in patients with advanced adenoma than controls (p=0.022). The marker , when combined with FIT, showed superior sensitivity (92.3% vs 73.1%, p<0.001) and area under the receiver-operating characteristic curve (AUC) (0.95 vs 0.86, p<0.001) than stand-alone FIT in detecting CRC in the same patient cohort. This combined test also increased the sensitivity (38.6% vs 15.5%, p<0.001) and AUC (0.65 vs 0.57, p=0.007) for detecting advanced adenoma. The performance gain for both CRC and advanced adenoma was confirmed in the validation cohort (p=0.0014 and p=0.031, respectively). CONCLUSIONS: This study identified marker as a valuable marker to improve diagnostic performance of FIT, providing a complementary role to detect lesions missed by FIT alone. This simple approach may improve the clinical utility of the current FIT, and takes one step further towards a non-invasive, potentially more accurate and affordable diagnosis of advanced colorectal neoplasia.
[Mh] Termos MeSH primário: Adenocarcinoma/diagnóstico
Adenoma/diagnóstico
Neoplasias Colorretais/diagnóstico
DNA Bacteriano/análise
Fezes/microbiologia
Fusobacterium nucleatum
Sangue Oculto
Peptostreptococcus
[Mh] Termos MeSH secundário: Área Sob a Curva
Biomarcadores Tumorais/análise
Feminino
Seres Humanos
Masculino
Meia-Idade
Curva ROC
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (DNA, Bacterial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE
[do] DOI:10.1136/gutjnl-2016-312766


  4 / 1314 MEDLINE  
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[PMID]:27149930
[Au] Autor:Chioma O; Aruni AW; Milford TA; Fletcher HM
[Ad] Endereço:Division of Microbiology and Molecular Genetics, Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, USA.
[Ti] Título:Filifactor alocis collagenase can modulate apoptosis of normal oral keratinocytes.
[So] Source:Mol Oral Microbiol;32(2):166-177, 2017 Apr.
[Is] ISSN:2041-1014
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:To successfully colonize host cells, pathogenic bacteria must circumvent the host's structural barrier such as the collagen-rich extracellular matrix (ECM), as a preliminary step to invasion and colonization of the periodontal tissue. Filifactor alocis possesses a putative Peptidase U32 family protein (HMPREF0389_00504) with collagenase activity that may play a significant role in colonization of host tissue during periodontitis by breaking down collagen into peptides and disruption of the host cell. Domain architecture of the HMPREF0389_00504 protein predicted the presence of a characteristic PrtC-like collagenase domain, and a peptidase domain. Our study demonstrated that the recombinant F. alocis peptidase U32 protein (designated PrtFAC) can interact with, and degrade, type I collagen, heat-denatured collagen and gelatin in a calcium-dependent manner. PrtFAC decreased viability and induced apoptosis of normal oral keratinocytes (NOKs) in a time and dose-dependent manner. Transcriptome analysis of NOK cells treated with PrtFAC showed an upregulation of the genes encoding human pro-apoptotic proteins: Apoptotic peptidase activating factor 1 (Apaf1) cytochrome C, as well as caspase 3 and caspase 9, suggesting the involvement of the mitochondrial apoptotic pathway. There was a significant increase in caspase 3/7 activity in NOK cells treated with PrtFAC. Taken together, these findings suggest that F. alocis PrtFAC protein may play a role in the virulence and pathogenesis of F. alocis.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Colágeno Tipo I/metabolismo
Colagenases/farmacologia
Queratinócitos/efeitos dos fármacos
Peptostreptococcus/enzimologia
[Mh] Termos MeSH secundário: Sequência de Bases
Células Cultivadas
Colagenases/química
Colagenases/isolamento & purificação
Colagenases/metabolismo
Células Epiteliais/efeitos dos fármacos
Gelatina/metabolismo
Perfilação da Expressão Gênica
Seres Humanos
Queratinócitos/citologia
Modelos Moleculares
Peptostreptococcus/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type I); 9000-70-8 (Gelatin); EC 3.4.24.- (Collagenases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE
[do] DOI:10.1111/omi.12163


  5 / 1314 MEDLINE  
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[PMID]:26408641
[Au] Autor:Yu J; Feng Q; Wong SH; Zhang D; Liang QY; Qin Y; Tang L; Zhao H; Stenvang J; Li Y; Wang X; Xu X; Chen N; Wu WK; Al-Aama J; Nielsen HJ; Kiilerich P; Jensen BA; Yau TO; Lan Z; Jia H; Li J; Xiao L; Lam TY; Ng SC; Cheng AS; Wong VW; Chan FK; Xu X; Yang H; Madsen L; Datz C; Tilg H; Wang J; Brünner N; Kristiansen K; Arumugam M; Sung JJ; Wang J
[Ad] Endereço:Department of Medicine & Therapeutics, State Key Laboratory of Digestive Disease, Institute of Digestive Disease, LKS Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong.
[Ti] Título:Metagenomic analysis of faecal microbiome as a tool towards targeted non-invasive biomarkers for colorectal cancer.
[So] Source:Gut;66(1):70-78, 2017 Jan.
[Is] ISSN:1468-3288
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate the potential for diagnosing colorectal cancer (CRC) from faecal metagenomes. DESIGN: We performed metagenome-wide association studies on faecal samples from 74 patients with CRC and 54 controls from China, and validated the results in 16 patients and 24 controls from Denmark. We further validated the biomarkers in two published cohorts from France and Austria. Finally, we employed targeted quantitative PCR (qPCR) assays to evaluate diagnostic potential of selected biomarkers in an independent Chinese cohort of 47 patients and 109 controls. RESULTS: Besides confirming known associations of Fusobacterium nucleatum and Peptostreptococcus stomatis with CRC, we found significant associations with several species, including Parvimonas micra and Solobacterium moorei. We identified 20 microbial gene markers that differentiated CRC and control microbiomes, and validated 4 markers in the Danish cohort. In the French and Austrian cohorts, these four genes distinguished CRC metagenomes from controls with areas under the receiver-operating curve (AUC) of 0.72 and 0.77, respectively. qPCR measurements of two of these genes accurately classified patients with CRC in the independent Chinese cohort with AUC=0.84 and OR of 23. These genes were enriched in early-stage (I-II) patient microbiomes, highlighting the potential for using faecal metagenomic biomarkers for early diagnosis of CRC. CONCLUSIONS: We present the first metagenomic profiling study of CRC faecal microbiomes to discover and validate microbial biomarkers in ethnically different cohorts, and to independently validate selected biomarkers using an affordable clinically relevant technology. Our study thus takes a step further towards affordable non-invasive early diagnostic biomarkers for CRC from faecal samples.
[Mh] Termos MeSH primário: Biomarcadores Tumorais
Neoplasias Colorretais/diagnóstico
Disbiose/microbiologia
Fezes/microbiologia
Microbioma Gastrointestinal/genética
[Mh] Termos MeSH secundário: Idoso
Área Sob a Curva
Áustria
Estudos de Casos e Controles
China
Estudos de Coortes
Neoplasias Colorretais/complicações
Dinamarca
Disbiose/complicações
Feminino
Firmicutes/isolamento & purificação
França
Fusobacterium nucleatum/isolamento & purificação
Estudo de Associação Genômica Ampla
Seres Humanos
Masculino
Metagenômica
Meia-Idade
Peptostreptococcus/isolamento & purificação
Curva ROC
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Biomarkers, Tumor)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:150927
[St] Status:MEDLINE
[do] DOI:10.1136/gutjnl-2015-309800


  6 / 1314 MEDLINE  
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[PMID]:27864301
[Au] Autor:García Carretero R; Luna-Heredia E; Olid-Velilla M; Vazquez-Gomez O
[Ad] Endereço:Hospital Universitario de Mostoles, Mostoles, Madrid, Spain.
[Ti] Título:Bacteraemia due to Parvimonas micra, a commensal pathogen, in a patient with an oesophageal tumour.
[So] Source:BMJ Case Rep;2016, 2016 Nov 18.
[Is] ISSN:1757-790X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A man aged 53 years was admitted to our hospital due to general malaise, fever and chills for the past 24 hours. He had a history of chronic alcoholic liver disease. The blood tests showed leucocytosis with neutrophilia, lactic acidosis and acute-phase reactants. The blood cultures were positive for Parvimonas micra, an anaerobic pathogen which is part of the flora of the oral cavity. There was no evidence of abscess formation in either the examination or the imaging tests, but in the work-up that followed, a gastroscopy showed a stenotic oesophageal mass that turned out to be an invasive squamous cell carcinoma.
[Mh] Termos MeSH primário: Bacteriemia/microbiologia
Carcinoma de Células Escamosas/complicações
Neoplasias Esofágicas/complicações
Esôfago/patologia
Bactérias Gram-Positivas/crescimento & desenvolvimento
Boca/microbiologia
[Mh] Termos MeSH secundário: Bacteriemia/complicações
Carcinoma de Células Escamosas/diagnóstico
Neoplasias Esofágicas/diagnóstico
Gastroscopia
Seres Humanos
Masculino
Meia-Idade
Peptostreptococcus
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE


  7 / 1314 MEDLINE  
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[PMID]:27756619
[Au] Autor:Liu M; Jin J; Pan H; Feng J; Cerniglia CE; Yang M; Chen H
[Ad] Endereço:Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72079, United States; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.
[Ti] Título:Effect of smokeless tobacco products on human oral bacteria growth and viability.
[So] Source:Anaerobe;42:152-161, 2016 Dec.
[Is] ISSN:1095-8274
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To evaluate the toxicity of smokeless tobacco products (STPs) on oral bacteria, seven smokeless tobacco aqueous extracts (STAEs) from major brands of STPs and three tobacco-specific N-nitrosamines (TSNAs) were used in a growth and viability test against 38 oral bacterial species or subspecies. All seven STAEs showed concentration-dependent effects on the growth and viability of tested oral bacteria under anaerobic culture conditions, although there were strain-to-strain variations. In the presence of 1 mg/ml STAEs, the growth of 4 strains decreased over 0.32-2.14 log fold, while 14 strains demonstrated enhanced growth of 0.3-1.76 log fold, and the growth of 21 strains was not significantly affected. In the presence of 10 mg/ml STAEs, the growth of 17 strains was inhibited 0.3-2.11 log fold, 18 strains showed enhanced growth of 0.3-0.97 log fold, and 4 strains were not significantly affected. In the presence of 50 mg/ml STAEs, the growth of 32 strains was inhibited 0.3-2.96 log fold, 8 strains showed enhanced growth of 0.3-1.0 log fold, and 2 strains were not significantly affected. All seven STAEs could promote the growth of 4 bacterial strains, including Eubacterium nodatum, Peptostreptococcus micros, Streptococcus anginosus, and Streptococcus constellatus. Exposure to STAEs modulated the viability of some bacterial strains, with 21.1-66.5% decrease for 4 strains at 1 mg/ml, 20.3-85.7% decrease for 10 strains at 10 mg/ml, 20.0-93.3% decrease for 27 strains at 50 mg/ml, and no significant effect for 11 strains at up to 50 mg/ml. STAEs from snuffs inhibited more tested bacterial strains than those from snus indicating that the snuffs may be more toxic to the oral bacteria than snus. For TSNAs, cell growth and viability of 34 tested strains were not significantly affected at up to 100 µg/ml; while the growth of P. micros was enhanced 0.31-0.54 log fold; the growth of Veillonella parvula was repressed 0.33-0.36 log fold; and the cell viabilities of 2 strains decreased 56.6-69.9%. The results demonstrate that STAEs affected the growth of some types of oral bacteria, which may affect the healthy ecological balance of oral bacteria in humans. On the other hand, TSNAs did not significantly affect the growth of the oral bacteria.
[Mh] Termos MeSH primário: Misturas Complexas/farmacologia
Microbiota/efeitos dos fármacos
Boca/microbiologia
Nitrosaminas/farmacologia
Tabaco sem Fumaça/análise
[Mh] Termos MeSH secundário: Meios de Cultura/química
Eubacterium/efeitos dos fármacos
Eubacterium/isolamento & purificação
Eubacterium/fisiologia
Seres Humanos
Concentração de Íons de Hidrogênio
Viabilidade Microbiana/efeitos dos fármacos
Microbiota/fisiologia
Peptostreptococcus/efeitos dos fármacos
Peptostreptococcus/isolamento & purificação
Peptostreptococcus/fisiologia
Especificidade da Espécie
Streptococcus anginosus/efeitos dos fármacos
Streptococcus anginosus/isolamento & purificação
Streptococcus anginosus/fisiologia
Streptococcus constellatus/efeitos dos fármacos
Streptococcus constellatus/isolamento & purificação
Streptococcus constellatus/fisiologia
Veillonella/efeitos dos fármacos
Veillonella/isolamento & purificação
Veillonella/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complex Mixtures); 0 (Culture Media); 0 (Nitrosamines)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  8 / 1314 MEDLINE  
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[PMID]:27555373
[Au] Autor:Testa M; Erbiti S; Delgado A; Cardenas IL
[Ad] Endereço:Microbiology and Parasitology Department, Faculty of Dentistry, National University of Tucuman, Argentina. Electronic address: mercedes.testa@gmail.com.
[Ti] Título:Evaluation of oral microbiota in undernourished and eutrophic children using checkerboard DNA-DNA hybridization.
[So] Source:Anaerobe;42:55-59, 2016 Dec.
[Is] ISSN:1095-8274
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate the relationship among nutritional status, gingival health and the composition of oral microbiota in children of a public school from a very poor area of San Miguel de Tucuman. Forty-five children ranging in age from 6 to 14 years old, 13 males and 32 females were studied. Twenty of these children were undernourished (Lejarraga-Morasso Table) and twenty-five were eutrophic. A clinical study that included DMF and dmf indexes, Löe Silness Plaque Index and bleeding on probing was performed. For microbiological study, saliva samples without stimulation were taken; aliquots of them were immediately placed in TAE buffer pH 7.6, adding NaOH (N and keeping at -70 °C until processed by checkerboard DNA-DNA hybridization method to check the presence of 40 oral microorganism species. Positive bleeding on probing was present in more than 80% of children, without significant differences between eutrophic and undernourished groups. Same result were obtain for the other clinical indexes (p > 0.05, Two Way ANOVA). Significant differences were found for some oral microorganism species, with a higher percentage of undernourished children harboring them. That was the case of S. gordonii (p < 0.05), Capnocitophaga gingivalis and C. ochraceae (p < 0.01 and p < 0.10, respectively), F. nucleatum ss nucleatum (p < 0.05), P. nigrescens (p < 0.10), Campylobacter gracilis (p < 0,05), and T. denticola (p < 0.10, multiple logistic regression). Significant differences were also found between children groups for E. saborreum (p < 0.001), P. acnes (p < 0.10), G. morbillorum (p < 0.05) and L. buccalis (p < 0.10). Gingivitis and bleeding on probing would not be related to nutritional status in the groups of children studied. There were significant differences for the presence of some of the main periodontal pathogen species between eutrophic and undernourished children. It would be important to study the meaning of significant differences found for the other microorganisms more deeply.
[Mh] Termos MeSH primário: DNA Bacteriano/genética
Gengiva/microbiologia
Gengivite/microbiologia
Desnutrição/microbiologia
Microbiota/genética
[Mh] Termos MeSH secundário: Adolescente
Aggregatibacter actinomycetemcomitans/classificação
Aggregatibacter actinomycetemcomitans/genética
Aggregatibacter actinomycetemcomitans/isolamento & purificação
Argentina
Bacteroides/classificação
Bacteroides/genética
Bacteroides/isolamento & purificação
Campylobacter/classificação
Campylobacter/genética
Campylobacter/isolamento & purificação
Capnocytophaga/classificação
Capnocytophaga/genética
Capnocytophaga/isolamento & purificação
Estudos de Casos e Controles
Criança
Feminino
Fusobacterium nucleatum/classificação
Fusobacterium nucleatum/genética
Fusobacterium nucleatum/isolamento & purificação
Gengivite/fisiopatologia
Seres Humanos
Masculino
Desnutrição/fisiopatologia
Hibridização de Ácido Nucleico
Peptostreptococcus/classificação
Peptostreptococcus/genética
Peptostreptococcus/isolamento & purificação
Porphyromonas gingivalis/classificação
Porphyromonas gingivalis/genética
Porphyromonas gingivalis/isolamento & purificação
Saliva/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:27471059
[Au] Autor:Harlow BE; Bryant RW; Cohen SD; O'Connell SP; Flythe MD
[Ad] Endereço:Department of Animal and Food Sciences, University of Kentucky, Lexington, KY, USA.
[Ti] Título:Degradation of spent craft brewer's yeast by caprine rumen hyper ammonia-producing bacteria.
[So] Source:Lett Appl Microbiol;63(4):307-12, 2016 Oct.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Spent yeast from craft beers often includes more hops (Humulus lupulus L.) secondary metabolites than traditional recipes. These compounds include α- and ß- acids, which are antimicrobial to the rumen hyper ammonia-producing bacteria (HAB) that are major contributors to amino acid degradation. The objective was to determine if the hops acids in spent craft brewer's yeast (CY; ~ 3·5 mg g(-1) hops acids) would protect it from degradation by caprine rumen bacteria and HAB when compared to a baker's yeast (BY; no hops acids). Cell suspensions were prepared by harvesting rumen fluid from fistulated goats, straining and differential centrifugation. The cells were re-suspended in media with BY or CY. After 24 h (39°C), HAB were enumerated and ammonia was measured. Fewer HAB and less ammonia was produced from CY than from BY. Pure culture experiments were conducted with Peptostreptococcus anaerobiusBG1 (caprine HAB). Ammonia production by BG1 from BY was greater than from CY. Ammonia production was greater when exogenous amino acids were included, but similar inhibition was observed in CY treatments. These results indicate that rumen micro-organisms deaminated the amino acids in CY to a lesser degree than BY. SIGNIFICANCE AND IMPACT OF THE STUDY: Spent brewer's yeast has long been included in ruminant diets as a protein supplement. However, modern craft beers often include more hops (Humulus lupulus L.) than traditional recipes. These compounds include α- and ß- acids, which are antimicrobial to the rumen hyper ammonia-producing bacteria (HAB) that are major contributors to amino acid degradation. This study demonstrated that hops acids in spent craft brewer's yeast protected protein from destruction by HABin vitro. These results suggest that the spent yeast from craft breweries, a source of beneficial hops secondary metabolites, could have value as rumen-protected protein.
[Mh] Termos MeSH primário: Amônia/metabolismo
Anti-Infecciosos/farmacologia
Cerveja/microbiologia
Humulus/química
Peptostreptococcus/metabolismo
Extratos Vegetais/farmacologia
Rúmen/microbiologia
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/química
Animais
Desaminação/fisiologia
Dieta
Cabras
Ruminantes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Anti-Infective Agents); 0 (Plant Extracts); 7664-41-7 (Ammonia)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160730
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12623


  10 / 1314 MEDLINE  
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Varanda, Eliana Aparecida
Bastos, Jairo Kenupp
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[PMID]:27452156
[Au] Autor:Leandro LF; Moraes Tda S; de Oliveira PF; Alves JM; Senedese JM; Ozelin SD; Resende FA; De Grandis RA; Varanda EA; Bastos JK; Tavares DC; Martins CH
[Ad] Endereço:1​ Laboratory of Research in Applied Microbiology, University of Franca - UNIFRAN, Franca, 14404-600 São Paulo, Brazil.
[Ti] Título:Assessment of the antibacterial, cytotoxic and mutagenic potential of the phenolic-rich hydroalcoholic extract from Copaifera trapezifolia Hayne leaves.
[So] Source:J Med Microbiol;65(9):937-50, 2016 Sep.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Copaifera trapezifolia Hayne occurs in the Atlantic Rainforest, which is considered one of the most important and endangered tropical forests on the planet. Although literature works have described many Copaifera spp., their biological activities remain little known. In the present study, we aimed to evaluate (1) the potential of the hydroalcoholic extract from C. trapezifolia leaves (CTE) to act against the causative agents of tooth decay and apical periodontitis and (2) the cytotoxicity and mutagenicity of CTE to ensure that it is safe for subsequent application. Concerning the tested bacteria, the MIC and the minimum bactericidal concentration of CTE varied between 100 and 400 µg ml-1. The time-kill assay conducted at a CTE concentration of 100 µg ml-1 evidenced bactericidal activity against Porphyromonas gingivalis (ATCC 33277) and Peptostreptococcus micros (clinical isolate) within 72 h. CTE at 200 µg ml-1 inhibited Porphyromonas gingivalis and Peptostreptococcus micros biofilm formation by at least 50 %. A combination of CTE with chlorhexidine dichlorohydrate did not prompt any synergistic effects. The colony-forming assay conducted on V79 cells showed that CTE was cytotoxic at concentrations above 156 µg ml-1. CTE exerted mutagenic effect on V79 cells, but the micronucleus test conducted on Swiss mice and the Ames test did not reveal any mutagenicity. Therefore, the use of standardized and safe extracts could be an important strategy to develop novel oral care products with antibacterial action. These extracts could also serve as a source of compounds for the discovery of new promising biomolecules.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antibacterianos/toxicidade
Produtos Biológicos/farmacologia
Produtos Biológicos/toxicidade
Fabaceae/química
Extratos Vegetais/farmacologia
Extratos Vegetais/toxicidade
[Mh] Termos MeSH secundário: Animais
Antibacterianos/isolamento & purificação
Biofilmes/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Cricetinae
Seres Humanos
Masculino
Camundongos
Testes de Sensibilidade Microbiana
Viabilidade Microbiana/efeitos dos fármacos
Testes de Mutagenicidade
Peptostreptococcus/efeitos dos fármacos
Peptostreptococcus/fisiologia
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Porphyromonas gingivalis/efeitos dos fármacos
Porphyromonas gingivalis/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Biological Products); 0 (Plant Extracts)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160726
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000316



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