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[PMID]:29245306
[Au] Autor:Chen D; Dong M; Zhao K; Sun F; Wang H; Liu Z
[Ad] Endereço:aThe First Affiliated Hospital of Medical School of Zhejiang University, Hangzhou, ZhejiangbKey Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province, Hangzhou, China.
[Ti] Título:Unusual synchronous liver and brain abscesses infected by rare Aerococcus viridians in a patient with pulmonary arteriovenous malformations on FDG PET/CT: A case report and literature review.
[So] Source:Medicine (Baltimore);96(49):e9048, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Pulmonary arteriovenous malformations (PAVMs) complicated with multiple organ abscesses is an uncommon manifestation. Because of the low incidence of the disease, F-18 fluorodeoxyglucose positron emission tomography with computed tomography (F-FDG PET/CT) imaging studies for PAVMs complicated with multiple organ abscesses are scarce. PATIENT CONCERNS: We report a case of a 54-year-old man presenting with PAVMs complicated with synchronous multiple organ abscesses founded by F-FDG PET/CT. F-FDG PET/CT revealed tortuous stripes and mass opacities with no significant FDG uptake in the left upper lung lobe. However, hypermetabolic lesions located in the anterior inferior segment of right hepatic lobe [with maximum standardized uptake value (SUVmax) of 10.7], and in the right basal ganglia with SUVmax of 14.1 were found by F-FDG PET/CT. DIAGNOSES: A diagnosis of synchronous liver and brain abscesses infected by rare Aerococcus viridans was determined by tissue culture. INTERVENTIONS: Vancomycin was provided intravenously, and oral linezolidate tablets were prescribed for anti-inflammatory treatment for 1 month. Liver and head magnetic resonance imaging was performed during the follow-up. OUTCOMES: The lesion in the right basal ganglia was reduced, and the lesion in the right liver had disappeared, indicating the lesions were abscesses. LESSONS: The present case indicated that the possibility of abscesses should be considered with patients with PAVMs, and whole-body F-FDG PET/CT is suggested to identify possible accompanying abscesses in multiple organs for PAVMs patients.
[Mh] Termos MeSH primário: Abscesso Encefálico/diagnóstico por imagem
Fluordesoxiglucose F18
Infecções por Bactérias Gram-Positivas/diagnóstico por imagem
Abscesso Hepático/diagnóstico por imagem
Tomografia Computadorizada com Tomografia por Emissão de Pósitrons/métodos
Compostos Radiofarmacêuticos
[Mh] Termos MeSH secundário: Aerococcus
Malformações Arteriovenosas/diagnóstico por imagem
Malformações Arteriovenosas/microbiologia
Abscesso Encefálico/microbiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Seres Humanos
Abscesso Hepático/microbiologia
Masculino
Meia-Idade
Artéria Pulmonar/anormalidades
Artéria Pulmonar/diagnóstico por imagem
Artéria Pulmonar/microbiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Radiopharmaceuticals); 0Z5B2CJX4D (Fluorodeoxyglucose F18)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009048


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[PMID]:28321788
[Au] Autor:Sun M; Gao J; Ali T; Yu D; Zhang S; Khan SU; Fanning S; Han B
[Ad] Endereço:Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, People's Republic of China.
[Ti] Título:Characteristics of Aerococcus viridans isolated from bovine subclinical mastitis and its effect on milk SCC, yield, and composition.
[So] Source:Trop Anim Health Prod;49(4):843-849, 2017 Apr.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aerococcus viridians (A. viridans), an environmental Gram-positive bacterium, has been documented to be associated with bovine mastitis. However, its exact role in bovine mastitis and the changes it brings about in milk characteristics are not yet known. The objectives of the current study were to describe the antibiotic resistance of A. viridans from bovine mastitis as well as the correlation between existence of this pathogen in udders and the somatic cell counts (SCC), daily milk yield, and composition of individual cow. One-year sampling for subclinical mastitis composite milk was conducted based on monthly DHI data from September 2013 to August 2014, in a commercial herd located in Beijing, China. All samples were cultured and pathogens were identified using microbiology method. A. viridians isolates were further identified by API identification system and 16S ribosomal RNA (rRNA) sequencing method. Kirby-Bauer disk diffusion method was used to test the antibiotic resistance of A. viridians against kinds of antimicrobial substance. SCC, milk yield, and milk composition data were from monthly Dairy Herd Improvement (DHI) results. Results showed that a total of 279 (16.67%) A. viridans isolates were identified from among 1674 bacterial isolates cultured from milk samples with high SCC. The incidence of mastitis caused by A. viridans was the highest (48-53%) during the summer season. Majority of the isolates were susceptible to most of antimicrobial compounds tested, especially to ß-lactams, but were found to be resistant (50-90%) to aminoglycosides, sulfonamides, and tetracycline. The average SCC of the A. viridans infected cows was significantly higher (1000.0 × 10 cells/mL) (P < 0.01) as compared to healthy cows (72.4 × 10 cells/mL) and daily milk yield decreased (P > 0.05) by 1.86 kg/day. Reductions were also observed in fat content (P > 0.05), lactose (P < 0.01), and total solids (P > 0.05), whereas protein content increased significantly (P < 0.01) in milk samples of cows infected with A. viridans. The results of this study suggest that A. viridans could be considered as an emerging aetiological agent of bovine subclinical mastitis wherein it exerts an effect on SCC, milk yield, and composition.
[Mh] Termos MeSH primário: Aerococcus/fisiologia
Farmacorresistência Bacteriana
Interações Hospedeiro-Patógeno
Lactação
Mastite Bovina/microbiologia
[Mh] Termos MeSH secundário: Aerococcus/isolamento & purificação
Animais
Antibacterianos
Bovinos
Contagem de Células/veterinária
China/epidemiologia
Feminino
Lactose/análise
Glândulas Mamárias Animais/microbiologia
Mastite Bovina/epidemiologia
Mastite Bovina/fisiopatologia
Testes de Sensibilidade Microbiana
Leite/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); J2B2A4N98G (Lactose)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1271-2


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[PMID]:27876382
[Au] Autor:Pundir CS; Aggarwal V
[Ad] Endereço:Department of Biochemistry, M.D. University, Rohtak 124001, Haryana, India. Electronic address: chandraspundir@gmail.com.
[Ti] Título:Amperometric triglyceride bionanosensor based on nanoparticles of lipase, glycerol kinase, glycerol-3-phosphate oxidase.
[So] Source:Anal Biochem;517:56-63, 2017 Jan 15.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The nanoparticles (NPs) aggregates of lipase from porcine pancreas, glycerol kinase (GK) from Cellulomonas sp. and glycerol-3-phosphate oxidase (GPO) from Aerococcus viridanss were prepared by desolvation and glutaraldehyde crosslinking and functionalized by cysteamine. These enzyme nanoparticles (ENPs) were characterized by transmission electron microscopy (TEM) and Fourier transform infra red (FTIR) spectroscopy. The functionalzed ENPs aggregates were co-immobilized covalently onto polycrystalline Au electrode through thiolated bond. An improved amperometric triglyceride (TG) bionanosensor was constructed using this ENPs modified Au electrode as working electrode. Biosensor showed optimum current at 1.2 V within 5s, at pH 6.5 and 35 °C.A linear relationship was obtained between current (mA) and triolein concentration in lower concentration range,10-100 mg/dL and higher concentration range, 100-500 mg/dL. Limit of detection (LOD) of bionanosensor was 1.0 µg/ml. Percent analytical recovery of added trolein (50 and 100 mg/dL) in serum was 95.2 ± 0.5 and 96.0 ± 0.17. Within and between batch coefficients of variation (CV) were 2.33% and 2.15% respectively. A good correlation (R = 0.99) was obtained between TG values in sera measured by present biosensor and standard enzymic colorimetric method with the regression equation: y= (0.993x + 0.967). ENPs/Au electrode was used 180 times over a period of 3 months with 50% loss in its initial activity, when stored dry at 4 °C.
[Mh] Termos MeSH primário: Aerococcus/enzimologia
Proteínas de Bactérias/química
Técnicas Biossensoriais/métodos
Cellulomonas/enzimologia
Glicerol Quinase/química
Glicerolfosfato Desidrogenase/química
Lipase/química
Nanopartículas/química
Triglicerídeos/sangue
[Mh] Termos MeSH secundário: Animais
Feminino
Seres Humanos
Masculino
Nanopartículas/ultraestrutura
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Triglycerides); EC 1.1.- (Glycerolphosphate Dehydrogenase); EC 1.1.3.21 (glycerol-3-phosphate oxidase); EC 2.7.1.30 (Glycerol Kinase); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


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[PMID]:27816682
[Au] Autor:Pan Z; Ma Y; Ma J; Dong W; Yao H
[Ad] Endereço:College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; OIE Reference Laboratory for Swine Streptococcosis, Nanjing 210095, China.
[Ti] Título:Acute meningitis of piglets and mice caused by co-infected with Streptococcus suis and Aerococcus viridans.
[So] Source:Microb Pathog;106:60-64, 2017 May.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The two opportunistic pathogens, Streptococcus suis (S. suis) and Aerococcus. viridans (A. viridans) were isolated from the brains of piglets suffered bacterial meningitis in a farm of China. The murine model has been established to evaluate the pathogenicity and symbiotic relationship of S. suis and A. viridans simultaneously infection. Our results demonstrated the ability of new serotype S. suis to cause the classical bacterial meningitis and death were greatly enhanced during co-infection with A. viridans in mice at a proportion. We also examined the distribution and titer of bacteria coinfection in organs, the titer of S. suis appeared a significant trend for an increase in the lung meanwhile the concentration titer of A. viridans maintain a low level. This is the first reported the A. viridans and S. suis coinfection cause the bacterial meningitis outbroke in the piglets and mice. Moreover, further investigation of the pathogenesis of A. viridans and S. suis is urgently needed in swine industry.
[Mh] Termos MeSH primário: Aerococcus/patogenicidade
Coinfecção/microbiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Meningite/microbiologia
Meningite/veterinária
Infecções Estreptocócicas/microbiologia
Streptococcus suis/patogenicidade
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Aerococcus/isolamento & purificação
Animais
Animais Domésticos/microbiologia
Encéfalo/microbiologia
Encéfalo/patologia
China/epidemiologia
Coinfecção/mortalidade
Coinfecção/patologia
Contagem de Colônia Microbiana
Modelos Animais de Doenças
Surtos de Doenças
Infecções por Bactérias Gram-Positivas/patologia
Infecções por Bactérias Gram-Positivas/veterinária
Coração/microbiologia
Rim/microbiologia
Rim/patologia
Dose Letal Mediana
Pulmão/microbiologia
Pulmão/patologia
Masculino
Meningite/epidemiologia
Meningite/mortalidade
Camundongos
Camundongos Endogâmicos BALB C
Infecções Estreptocócicas/patologia
Infecções Estreptocócicas/veterinária
Streptococcus suis/isolamento & purificação
Suínos
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:27645966
[Au] Autor:Kotkar KD; Said SM; Michelena H; Wanta B; Fritock MD; Baddour LM
[Ad] Endereço:Division of Cardiovascular Surgery, Mayo Clinic, Rochester, Minnesota.
[Ti] Título:Right Coronary Artery Septic Embolization Secondary to Aerococcus urinae Native Mitral Valve Endocarditis.
[So] Source:Ann Thorac Surg;102(4):e295-7, 2016 Oct.
[Is] ISSN:1552-6259
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Septic embolization to the coronary arteries from infective endocarditis is a rare occurrence. Appropriate treatment is not yet fully determined. We report a case of mitral valve endocarditis due to Aerococcus urinae presenting as acute inferior myocardial infarction.
[Mh] Termos MeSH primário: Aerococcus/isolamento & purificação
Trombose Coronária/cirurgia
Endocardite Bacteriana/complicações
Infecções por Bactérias Gram-Positivas/complicações
Insuficiência da Valva Mitral/cirurgia
Infarto do Miocárdio/diagnóstico
[Mh] Termos MeSH secundário: Bacteriemia/complicações
Bacteriemia/diagnóstico
Ponte de Artéria Coronária/métodos
Trombose Coronária/complicações
Trombose Coronária/diagnóstico
Diagnóstico Diferencial
Endocardite Bacteriana/diagnóstico
Seguimentos
Infecções por Bactérias Gram-Positivas/diagnóstico
Implante de Prótese de Valva Cardíaca/métodos
Seres Humanos
Masculino
Meia-Idade
Valva Mitral/microbiologia
Valva Mitral/cirurgia
Insuficiência da Valva Mitral/diagnóstico por imagem
Insuficiência da Valva Mitral/etiologia
Infarto do Miocárdio/etiologia
Medição de Risco
Trombectomia/métodos
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE


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[PMID]:27451085
[Au] Autor:Hirzel C; Hirzberger L; Furrer H; Endimiani A
[Ad] Endereço:Department of Infectious Diseases, Bern University Hospital (Inselspital), University of Bern, Poliklinik Trakt 2, CH-3010 Bern, Switzerland.
[Ti] Título:Bactericidal activity of penicillin, ceftriaxone, gentamicin and daptomycin alone and in combination against Aerococcus urinae.
[So] Source:Int J Antimicrob Agents;48(3):271-6, 2016 Sep.
[Is] ISSN:1872-7913
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Aerococcus urinae can cause severe infections (bacteraemia and endocarditis) that are associated with high mortality. However, data on the bactericidal and synergistic activity for clinically implemented antibiotics are scarce. Time-kill analyses were performed on two clinical isolates (AU1 and AU2) and the reference strain ATCC 700306 for penicillin (PG), ceftriaxone (CRO), gentamicin (GEN), daptomycin (DAP) and their combinations. AU1 and AU2 were CRO-resistant (MICs, 2 µg/mL) and ATCC 700306 was high-level GEN-resistant (MIC, 512 µg/mL), whereas all strains were PG- and DAP-susceptible (MICs, ≤0.125 and ≤1 µg/mL, respectively). CFU counts were determined at various time points from 0 to 48 h. All experiments were performed at 0.5×, 1×, 2× and 4× MIC. PG and CRO were not bactericidal for all strains, whereas DAP exhibited bactericidal activity at all concentrations for AU2 and ATCC 700306. The combination of PG or CRO with GEN was bactericidal for AU1 and AU2 at antibiotic concentrations ≥1× MIC. Bactericidal synergism was detected for PG or CRO combined with GEN in the two clinical isolates. PG plus CRO showed non-bactericidal synergism for ATCC 700306. DAP with GEN was synergistic at 1× MIC for AU1, whereas the killing activity of DAP was too pronounced to detect potential synergism in AU2. The combination of PG or CRO with GEN is synergistic and bactericidal. Moreover, these in vitro data suggest that DAP may represent a potential bactericidal treatment alternative against A. urinae. This finding could be important for the treatment of patients with a ß-lactam allergy or renal insufficiency.
[Mh] Termos MeSH primário: Aerococcus/efeitos dos fármacos
Antibacterianos/farmacologia
Daptomicina/farmacologia
Sinergismo Farmacológico
Gentamicinas/farmacologia
Viabilidade Microbiana/efeitos dos fármacos
beta-Lactamas/farmacologia
[Mh] Termos MeSH secundário: Aerococcus/isolamento & purificação
Aerococcus/fisiologia
Idoso
Idoso de 80 Anos ou mais
Contagem de Colônia Microbiana
Feminino
Infecções por Bactérias Gram-Positivas/microbiologia
Seres Humanos
Testes de Sensibilidade Microbiana
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Gentamicins); 0 (beta-Lactams); NWQ5N31VKK (Daptomycin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE


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[PMID]:27440847
[Au] Autor:Melnick S; Nazir S; Hingorani R; Wexler P
[Ad] Endereço:Department of Internal Medicine, Reading Hospital and Medical Center, Reading, Pennsylvania, USA Lebanon, Pennsylvania, USA.
[Ti] Título:Aerococcus urinae, a rare cause of infective endocarditis.
[So] Source:BMJ Case Rep;2016, 2016 Jul 20.
[Is] ISSN:1757-790X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We present the case of an elderly male who was initially seen in our hospital for a urinary tract infection that was treated with oral ciprofloxacin. He was admitted 2 weeks later with altered mental status and fever, and was found to have bacteraemia with Aerococcus urinae Owing to altered mental status a brain MRI was performed which showed evidence of embolic stroke. Following this, a transesophageal echocardiogram showed severe mitral regurgitation and a vegetation >1 cm involving the mitral valve with associated destruction of posterior valve leaflets. The patient was started on antibiotics intravenous penicillin G and intravenous gentamicin for a total duration of 6 weeks. He underwent mitral valve replacement on day 4 of hospitalisation. The postoperative course was complicated by ventilator-dependent respiratory failure, requiring tracheostomy and eventual transfer to a skilled nursing facility. Unfortunately, he died after 2 weeks of stay at the facility.
[Mh] Termos MeSH primário: Endocardite Bacteriana/diagnóstico
Endocardite Bacteriana/etiologia
Insuficiência da Valva Mitral/microbiologia
Infecções Urinárias/complicações
[Mh] Termos MeSH secundário: Aerococcus
Idoso
Antibacterianos/uso terapêutico
Encéfalo/diagnóstico por imagem
Ecocardiografia Transesofagiana
Endocardite Bacteriana/tratamento farmacológico
Evolução Fatal
Gentamicinas/uso terapêutico
Seres Humanos
Imagem por Ressonância Magnética
Masculino
Valva Mitral/microbiologia
Valva Mitral/cirurgia
Insuficiência da Valva Mitral/cirurgia
Penicilinas/uso terapêutico
Acidente Vascular Cerebral/complicações
Infecções Urinárias/diagnóstico
Infecções Urinárias/tratamento farmacológico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Gentamicins); 0 (Penicillins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170303
[Lr] Data última revisão:
170303
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160722
[St] Status:MEDLINE


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[PMID]:26868516
[Au] Autor:Lee MY; Kim MH; Lee WI; Kang SY; Jeon YL
[Ad] Endereço:Department of Laboratory Medicine, Graduate School, Kyung Hee University, Seoul, South Korea and Department of Laboratory Medicine, School of Medicine, Kyung Hee University and Kyung Hee University Hospital at Gangdong, Seoul, South Korea.
[Ti] Título:A Case of Sepsis in a 92-Year-Old Korean Woman Caused by Aerococcus urinae and Identified by Sequencing the 16S Ribosomal RNA Gene.
[So] Source:Lab Med;47(2):e15-7, 2016 May.
[Is] ISSN:1943-7730
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aerococcus urinae is an uncommon pathogen that was first identified in 1992. Herein, we report a case of bloodstream infection caused by A. urinae, which occurred in a 92-year-old Korean female patient with an underlying urologic infection who had altered consciousness. The blood culture yielded positive results for A. urinae; however, identifying A. urinae was challenging. Ultimately, we used 16S ribosomal RNA (rRNA) gene sequencing to identify the organism. The patient recovered after being treated with ertapenem and meropenem. To our knowledge, this is the first report of a case of A. urinae sepsis in South Korea.
[Mh] Termos MeSH primário: Aerococcus/genética
Infecções por Bactérias Gram-Positivas/diagnóstico
RNA Ribossômico 16S/análise
Sepse/diagnóstico
Infecções Urinárias/diagnóstico
[Mh] Termos MeSH secundário: Idoso de 80 Anos ou mais
Antibacterianos/uso terapêutico
Feminino
Infecções por Bactérias Gram-Positivas/tratamento farmacológico
Seres Humanos
Indução de Remissão
Sepse/tratamento farmacológico
Análise de Sequência de RNA
Tienamicinas/uso terapêutico
beta-Lactamas/uso terapêutico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA, Ribosomal, 16S); 0 (Thienamycins); 0 (beta-Lactams); FV9J3JU8B1 (meropenem); G32F6EID2H (ertapenem)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161231
[Lr] Data última revisão:
161231
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE
[do] DOI:10.1093/labmed/lmv030


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[PMID]:26854338
[Au] Autor:Moreno LZ; Matajira CE; Gomes VT; Silva AP; Mesquita RE; Christ AP; Sato MI; Moreno AM
[Ad] Endereço:Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Av Prof Dr Orlando Marques de Paiva, 87; Cidade Universitária. São Paulo/SP, Brazil. Electronic address: luzanolli@gmail.com.
[Ti] Título:Molecular and antibiotic susceptibility characterization of Aerococcus viridans isolated from porcine urinary infection.
[So] Source:Vet Microbiol;184:7-10, 2016 Feb 29.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Aerococcus viridans has been reported as a human and animal pathogen causing urinary tract infection, arthritis, pneumonia, meningitis and endocarditis. Routinely, A. viridans is not surveyed in clinical diagnosis laboratories and commonly is misidentified as other bacteria. There is no concrete data on the prevalence and impact of the pathogen to both human and animal health. In the present study, we report the isolation and molecular and antibiotic susceptibility characterization of A. viridans strains from porcine urinary infections. A total of 22 isolates were identified as A. viridans by MALDI-TOF MS and confirmed by 16S rRNA gene sequencing. Isolates were genotyped by single enzyme amplified fragments length polymorphism (SE-AFLP) that resulted in 19 clusters of which 81.2% were composed by single isolates. The high genetic heterogeneity corroborates previous studies and appears to be a particularity of A. viridans. The minimal inhibitory concentration (MIC) values also presented variability especially for ceftiofur, fluoroquinolones and aminoglycosides. The high MICs of aminoglycosides, tetracyclines and macrolides seen among the A. viridans corroborate previous reports and the widespread veterinary usage of these antibiotics demand attention for the implication of A. viridans infection to both human and animal health.
[Mh] Termos MeSH primário: Aerococcus/efeitos dos fármacos
Aerococcus/genética
Antibacterianos/farmacologia
Infecções Urinárias/veterinária
[Mh] Termos MeSH secundário: Aerococcus/isolamento & purificação
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Animais
Genótipo
Testes de Sensibilidade Microbiana
RNA Ribossômico 16S
Suínos
Doenças dos Suínos/microbiologia
Infecções Urinárias/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE


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[PMID]:26838685
[Au] Autor:Senneby E; Göransson L; Weiber S; Rasmussen M
[Ad] Endereço:Clinical Microbiology, Lund, Region Skåne, Sweden. erik.senneby@med.lu.se.
[Ti] Título:A population-based study of aerococcal bacteraemia in the MALDI-TOF MS-era.
[So] Source:Eur J Clin Microbiol Infect Dis;35(5):755-62, 2016 May.
[Is] ISSN:1435-4373
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was to determine the incidence of aerococcal bacteraemia in the MALDI-TOF MS-era, to describe the clinical presentation and to determine the MIC values of aerococci for ten antibiotics. Aerococci in blood cultures were identified through searches in the laboratory database for the years 2012-2014. MALDI-TOF MS, sequencing of the 16S rRNA gene and a PYR test were used for species identification. Patients' medical charts were systematically reviewed. Etests were used to determine MIC values. Seventy-seven patients were identified (Aerococcus urinae n = 49, Aerococcus viridans n = 14, Aerococcus sanguinicola n = 13 and Aerococcus christensenii n = 1) corresponding to incidences of 14 cases per 1,000,000 inhabitants per year (A. urinae) and 3.5 cases per 1,000,000 inhabitants per year (A. sanguinicola and A.viridans). A. urinae was in pure culture in 61 %, A. sanguinicola in 46 % and A. viridans in 36 % of the cases. The A. urinae and A. sanguinicola patients were old and many had urinary tract disorders, and a majority had a suspected urinary tract focus of the bacteraemia. Eighty percent of the A. urinae patients were men. Five A. urinae patients were diagnosed with infective endocarditis. Six patients died within 30 days. Most isolates had low MICs to penicillins and carbapenems. MALDI-TOF MS has led to an increased identification of aerococcal bacteremia. A. urinae remains the most common Aerococcus in blood cultures and in aerococcal IE.
[Mh] Termos MeSH primário: Aerococcus/classificação
Bacteriemia
Infecções por Bactérias Gram-Positivas/epidemiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Vigilância da População
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
[Mh] Termos MeSH secundário: Adolescente
Adulto
Aerococcus/efeitos dos fármacos
Idoso
Idoso de 80 Anos ou mais
Antibacterianos/farmacologia
Criança
Pré-Escolar
Comorbidade
Feminino
Infecções por Bactérias Gram-Positivas/diagnóstico
Infecções por Bactérias Gram-Positivas/tratamento farmacológico
Seres Humanos
Lactente
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Mortalidade
Estudos Retrospectivos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE
[do] DOI:10.1007/s10096-016-2594-z



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