Base de dados : MEDLINE
Pesquisa : B03.353.750.250.250.280 [Categoria DeCS]
Referências encontradas : 9192 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 920 ir para página                         

  1 / 9192 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29215338
[Au] Autor:Kart D; Kustimur AS; Sagiroglu M; Kalkanci A
[Ad] Endereço:Department of Pharmaceutical Microbiology, Hacettepe University Faculty of Pharmacy, Ankara, Turkey.
[Ti] Título:Evaluation of Antimicrobial Durability and Anti-Biofilm Effects in Urinary Catheters Against Clinical Isolates and Reference Strains.
[So] Source:Balkan Med J;34(6):546-552, 2017 12 01.
[Is] ISSN:2146-3131
[Cp] País de publicação:Turkey
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: and biofilms are major causes of catheter-associated urinary tract infections. Antimicrobial-coated or impregnated urinary catheters are seen as a possible way to prevent these infections. AIMS: To determine the biofilm-forming ability of 89 isolates from urinary tract infections and to compare several urinary catheters for antimicrobial durability and the inhibitory effects on biofilm formation of different laboratory strains and clinical isolates of . STUDY DESIGN: experimental study. METHODS: The biofilm forming ability of isolates was determined by the crystal violet staining and plate counting methods. For comparison of urinary catheters, biofilms of 45 isolates from the catheter samples of hospitalized patients and five laboratory strains of ATCC25922, ATCC35984, ATCC27853, ATCC29212 and ATCC90028 were formed on the catheters in 24-well tissue culture plates. Scanning electron microscopy analysis was performed to observe biofilms. RESULTS: All 89 isolates were found to be biofilm positive. Nitrofurazone-impregnated catheters significantly reduced the cell counts of isolates and completely inhibited the formation of and biofilms compared with the others. Regarding reduction of biofilm cell counts, a hydrophilic-coated catheter was more effective against , whereas a silver-coated catheter was found to be more effective against . The nitrofurazone-impregnated catheter had the best antimicrobial durability. CONCLUSION: Urine isolates of had considerable ability with respect to biofilm formation. The nitrofurazone-impregnated catheter was the most effective against all tested bacteria; however, the effect of a hydrophilic or silver-coated catheter depends on the species present in it.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Biofilmes/efeitos dos fármacos
Infecções Relacionadas a Cateter/microbiologia
Cateteres de Demora/microbiologia
Enterococcus faecalis/efeitos dos fármacos
Nitrofurazona/farmacologia
Infecções Urinárias/microbiologia
[Mh] Termos MeSH secundário: Infecções Relacionadas a Cateter/prevenção & controle
Materiais Revestidos Biocompatíveis
Enterococcus faecalis/isolamento & purificação
Seres Humanos
Técnicas In Vitro
Testes de Sensibilidade Microbiana
Silicones
Prata
Cateterismo Urinário/efeitos adversos
Infecções Urinárias/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Coated Materials, Biocompatible); 0 (Silicones); 3M4G523W1G (Silver); X8XI70B5Z6 (Nitrofurazone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.4274/balkanmedj.2016.1853


  2 / 9192 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29361927
[Au] Autor:Lin Z; Feng X; Zheng L; Moonasar N; Shen L; Wu R; Chen F
[Ad] Endereço:The Eye Hospital, School of Ophthalmology and Optometry, Wenzhou Medical University, No. 270 West College Road, Wenzhou, Zhejiang, 325027, China.
[Ti] Título:Incidence of endophthalmitis after 23-gauge pars plana vitrectomy.
[So] Source:BMC Ophthalmol;18(1):16, 2018 Jan 23.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Endophthalmitis is a rare but severe complication following PPV. The incidence of endophthalmitis varies between 20-gauge, 23-gauge, and 25-gauge incisions. The incidence and clinical features of endophthalmitis after 23-gauge PPV in an eye hospital in China was reported in this study. METHODS: Data of the eyes that underwent 23-gauge PPV from January 2011 to December 2014 at the Eye Hospital of Wenzhou Medical University was retrospectively collected. All the information was obtained from the electronic medical system. The exclusion criteria included: (1) preoperative diagnosis of endophthalmitis; (2) history of vitrectomy; (3) intraocular surgery within 6 months; (4) history of ocular penetrating trauma; (5) sutures for any of the 3 sclerotomy incisions; (6) patients with cancer, acquired immune deficiency syndrome, or taking drugs that may influence the immune system. The diagnosis of endophthalmitis was based on clinical characteristics and/or culture results from an operative sample. RESULTS: Three thousand nine hundred seventy nine eyes that underwent 23-gauge PPV surgery were included in this study. Among these eyes, 3 eyes developed endophthalmitis after surgery, giving an incidence of 0.075% (3/3979). The period in which endophthalmitis developed ranged from 1 to 5 days post-operation. The visual acuity decreased to hand motions or light perception postoperatively. The culture of aqueous and vitreous of the 2 eyes revealed Staphylococcus epidermidis and enterococcus faecalis respectively, however was negative for the third eye. All 3 eyes had a favorable response to the treatment of vitreous tap and intravitreal antibiotics injection. Two eyes gained visual acuity of 0.05 and 0.5, respectively at the final visit. CONCLUSIONS: Endophthalmitis is a rare but sight-threatening complication after 23-gauge pars plana vitrectomy. The peak duration of onset was within 5 days post-operation, with gram positive cocci being the common pathogenic organism.
[Mh] Termos MeSH primário: Endoftalmite/epidemiologia
Infecções Oculares Bacterianas/epidemiologia
Microcirurgia/efeitos adversos
Doenças Retinianas/cirurgia
Infecções Estafilocócicas/epidemiologia
Infecção da Ferida Cirúrgica/epidemiologia
Vitrectomia/efeitos adversos
[Mh] Termos MeSH secundário: Adulto
Idoso
Humor Aquoso/microbiologia
China/epidemiologia
Endoftalmite/diagnóstico
Endoftalmite/microbiologia
Enterococcus faecalis/isolamento & purificação
Infecções Oculares Bacterianas/diagnóstico
Infecções Oculares Bacterianas/microbiologia
Feminino
Seguimentos
Infecções por Bactérias Gram-Positivas/diagnóstico
Infecções por Bactérias Gram-Positivas/epidemiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Seres Humanos
Incidência
Masculino
Microcirurgia/instrumentação
Meia-Idade
Estudos Retrospectivos
Infecções Estafilocócicas/diagnóstico
Infecções Estafilocócicas/microbiologia
Staphylococcus epidermidis/isolamento & purificação
Infecção da Ferida Cirúrgica/microbiologia
Ultrassonografia
Acuidade Visual
Vitrectomia/instrumentação
Corpo Vítreo/diagnóstico por imagem
Corpo Vítreo/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0678-5


  3 / 9192 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Bramante, Clóvis Monteiro
Texto completo SciELO Brasil
[PMID]:29211285
[Au] Autor:Alcalde MP; Bramante CM; Vivan RR; Amorso-Silva PA; Andrade FB; Duarte MAH
[Ad] Endereço:Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Dentística, Endodontia e Materiais Odontológicos, Bauru, SP, Brasil.
[Ti] Título:Intradentinal antimicrobial action and filling quality promoted by ultrasonic agitation of epoxy resin-based sealer in endodontic obturation.
[So] Source:J Appl Oral Sci;25(6):641-649, 2017 Nov-Dec.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:MATERIAL AND METHODS: The aim of this study was evaluate the influence of ultrasonic activation (UA) of AH Plus to improve canal and isthmus filing, and analyse the antimicrobial effect against Enterococcus faecalis within dentinal tubules. Thirty mesial roots of mandibular first molars were selected and divided into 2 groups (n = 15): with and without UA of the sealer. Then the root canals were filled by using the single cone technique, and the specimens were sectioned at 2, 4 and 6 mm from the apex for stereomicroscope and confocal laser scanner microscopy (CLSM) analysis. In addition, 30 bovine incisors were contaminated with Enterococcus faecalis and divided into 3 groups (n = 10). The specimens were obturated by using the single cone technique with (G1) and without (G2) UA of the sealer and G3 as the control group. All were sectioned into 6 mm-long cylinders and stained with LIVE/DEAD to assess bacterial viability by CLSM. RESULTS: The UA of the sealer significantly reduced the presence of unfilled areas in the canal and isthmus area in all sections (p<0.05), and there was a significant increase in sealer penetration in both canals and isthmuses (p<0.05). As regards gaps, a significant reduction was found at 2 and 6 mm in the isthmus area of the UA group (p<0.05). Moreover, UA of the sealer significantly reduced bacterial viability in the superficial dentine when compared with the other groups (p<0.05). CONCLUSION: Ultrasonic activation of the AH Plus sealer promoted a better quality of root canal filling and increased the intratubular penetration of sealer, especially in the isthmus area. Additionally, ultrasonic activation of the sealer increased the intradentinal antimicrobial action against Enterococcus faecalis, mainly in the superficial dentine of the root canal.
[Mh] Termos MeSH primário: Cavidade Pulpar/microbiologia
Enterococcus faecalis/efeitos dos fármacos
Resinas Epóxi
Materiais Restauradores do Canal Radicular/farmacologia
Obturação do Canal Radicular/métodos
Ultrassom
[Mh] Termos MeSH secundário: Seres Humanos
Microscopia Confocal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Epoxy Resins); 0 (Root Canal Filling Materials)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  4 / 9192 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29353702
[Au] Autor:Manoil D; Lange N; Bouillaguet S
[Ad] Endereço:Endodontics Unit, Section of Dental Medicine, University of Geneva, 1, rue Michel-Servet, CH-1206 Geneva, Switzerland. Electronic address: Daniel.Manoil@unige.ch.
[Ti] Título:Enzyme-mediated photoinactivation of Enterococcus faecalis using Rose Bengal-acetate.
[So] Source:J Photochem Photobiol B;179:84-90, 2018 Feb.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Rose Bengal-acetate (RB-Ac) is a pro-photosensitizer claimed to diffuse into target cells, where the acetate groups are hydrolyzed and the photosensitizing properties of Rose Bengal (RB) are restored. Despite promising results on tumor cells, the interaction of RB-Ac with bacteria has never been investigated. This study aimed to assess the interaction of RB-Ac with Enterococcus faecalis and to evaluate its potential use in antimicrobial photodynamic therapy (aPDT). Spectrofluorometry was used to assess the ability of E. faecalis to hydrolyze the RB-Ac compound. Fluorescence microscopy was employed to observe the distribution and to evaluate the cellular uptake of the RB produced. The antibacterial efficiency of RB-Ac-mediated aPDT was assessed by flow cytometry in combination with the LIVE/DEAD® staining. Results showed that RB-Ac was successfully hydrolyzed in the presence of E. faecalis cells. The RB produced appeared to incorporate the membrane of bacteria. Higher concentrations of RB-Ac resulted in higher incorporation of RB. The blue-light irradiation of RB-Ac-treated samples significantly reduced bacterial viability. Less than 0.01% of E. faecalis survived after incubation with 200 µM RB-Ac during 900 min and blue-light activation. The current report indicates that E. faecalis cells can hydrolyze the RB-Ac compound to produce active RB. The use of RB-Ac did not appear to allow cytoplasmic internalization of the RB produced, which rather incorporated the membrane bilayers of E. faecalis. The use of RB-Ac did not provide additional advantages over RB in terms of PS localization. Nonetheless, sufficient RB was produced and incorporated into the membranes of bacteria to elicit effective aPDT.
[Mh] Termos MeSH primário: Enterococcus faecalis/efeitos dos fármacos
Fármacos Fotossensibilizantes/farmacologia
Rosa Bengala/análogos & derivados
[Mh] Termos MeSH secundário: Enterococcus faecalis/efeitos da radiação
Hidrólise/efeitos da radiação
Luz
Viabilidade Microbiana/efeitos dos fármacos
Viabilidade Microbiana/efeitos da radiação
Microscopia de Fluorescência
Rosa Bengala/farmacologia
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosensitizing Agents); 0 (Rose Bengal acetate); 1ZPG1ELY14 (Rose Bengal)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE


  5 / 9192 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29385177
[Au] Autor:Dickel F; Münch D; Amdam GV; Mappes J; Freitak D
[Ad] Endereço:Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, Finland.
[Ti] Título:Increased survival of honeybees in the laboratory after simultaneous exposure to low doses of pesticides and bacteria.
[So] Source:PLoS One;13(1):e0191256, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent studies of honeybees and bumblebees have examined combinatory effects of different stressors, as insect pollinators are naturally exposed to multiple stressors. At the same time the potential influences of simultaneously occurring agricultural agents on insect pollinator health remain largely unknown. Due to different farming methods, and the drift of applied agents and manure, pollinators are most probably exposed to insecticides but also bacteria from organic fertilizers at the same time. We orally exposed honeybee workers to sub-lethal doses of the insecticide thiacloprid and two strains of the bacterium Enterococcus faecalis, which can occur in manure from farming animals. Our results show that under laboratory conditions the bees simultaneously exposed to the a bacterium and the pesticide thiacloprid thiacloprid had significant higher survival rates 11 days post exposure than the controls, which surprisingly showed the lowest survival. Bees that were exposed to diet containing thiacloprid showed decreased food intake. General antibacterial activity is increased by the insecticide and the bacteria, resulting in a higher immune response observed in treated individuals compared to control individuals. We thus propose that caloric restriction through behavioural and physiological adaptations may have mediated an improved survival and stress resistance in our tests. However, the decreased food consumption could in long-term also result in possible negative effects at colony level. Our study does not show an additive negative impact of sub-lethal insecticide and bacteria doses, when tested under laboratory conditions. In contrast, we report seemingly beneficial effects of simultaneous exposure of bees to agricultural agents, which might demonstrate a surprising biological capacity for coping with stressors, possibly through hormetic regulation.
[Mh] Termos MeSH primário: Abelhas/efeitos dos fármacos
Abelhas/microbiologia
Enterococcus faecalis/patogenicidade
Inseticidas/toxicidade
Neonicotinoides/toxicidade
Tiazinas/toxicidade
[Mh] Termos MeSH secundário: Animais
Abelhas/fisiologia
Ingestão de Alimentos/efeitos dos fármacos
Hormese
Inseticidas/administração & dosagem
Neonicotinoides/administração & dosagem
Polinização
Estresse Fisiológico
Tiazinas/administração & dosagem
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insecticides); 0 (Neonicotinoids); 0 (Thiazines); DSV3A944A4 (thiacloprid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191256


  6 / 9192 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28467378
[Au] Autor:Ali L; Goraya MU; Arafat Y; Ajmal M; Chen JL; Yu D
[Ad] Endereço:College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. liaqatpaksw@yahoo.com.
[Ti] Título:Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches.
[So] Source:Int J Mol Sci;18(5), 2017 May 03.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Quorum-sensing systems control major virulence determinants in , which causes nosocomial infections. The . quorum-sensing systems include several virulence factors that are regulated by the operon, which encodes the cytolysin toxin. In addition, the . Fsr regulator system controls the expression of gelatinase, serine protease, and enterocin O16. The cytolysin and Fsr virulence factor systems are linked to enterococcal diseases that affect the health of humans and other host models. Therefore, there is substantial interest in understanding and targeting these regulatory pathways to develop novel therapies for enterococcal infection control. Quorum-sensing inhibitors could be potential therapeutic agents for attenuating the pathogenic effects of . . Here, we discuss the regulation of cytolysin, the LuxS system, and the Fsr system, their role in . -mediated infections, and possible therapeutic approaches to prevent . infection.
[Mh] Termos MeSH primário: Infecção Hospitalar/microbiologia
Enterococcus faecalis/patogenicidade
Infecções por Bactérias Gram-Positivas/microbiologia
Percepção de Quorum/fisiologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Antibacterianos/uso terapêutico
Proteínas de Bactérias/genética
Biofilmes/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Liases de Carbono-Enxofre/genética
Infecção Hospitalar/tratamento farmacológico
Enterococcus faecalis/efeitos dos fármacos
Enterococcus faecalis/genética
Regulação Bacteriana da Expressão Gênica/fisiologia
Infecções por Bactérias Gram-Positivas/tratamento farmacológico
Seres Humanos
Perforina/genética
Percepção de Quorum/efeitos dos fármacos
Virulência
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Virulence Factors); 126465-35-8 (Perforin); EC 4.4.- (Carbon-Sulfur Lyases); EC 4.4.1.21 (LuxS protein, Bacteria)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  7 / 9192 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29217301
[Au] Autor:Bruneau A; Baylatry MT; Joly AC; Sokol H
[Ad] Endereço:AP-HP, hôpital Saint-Antoine, pharmacie hospitaliere, 184, rue du faubourg Saint-Antoine, 75012 Paris, France.
[Ti] Título:[Gut microbiota: What impact on colorectal carcinogenesis and treatment?]
[Ti] Título:Le microbiote intestinal : quels impacts sur la carcinogenèse et le traitement du cancer colorectal ?.
[So] Source:Bull Cancer;105(1):70-80, 2018 Jan.
[Is] ISSN:1769-6917
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:The gut microbiota, composed of 10 microorganisms, is now considered as a "hidden organ", regarding to its digestive, metabolic and immune functions, which are helpful to its host. For the last 15 years, advances in molecular biology have highlighted the association of gut microbiota dysbiosis with several diseases, including colorectal cancer. An increased abundance of some bacteria (including Fusobacterium nucleatum, Bacteroides fragilis, Escherichia coli) is associated with cancer, whereas others seem to be protective (Faecalibacterium prausnitzii). Several mechanisms, which are species-specific, are involved in colorectal carcinogenesis. Most of the time, bacterial toxins are involved in pro-inflammatory processes and in activation of angiogenesis and cellular proliferation pathways. The identification of these bacteria leads to envisage the gut microbiota as potential screening tool for colorectal cancer. Recent studies showed a relation between the gut microbiota and the efficacy and toxicity of chemotherapies (oxaliplatin, irinotecan) and immunotherapies (including ipilimumab). Therapeutic approaches targeting the gut microbiota are now available (probiotics, fecal microbiota transplantation…). New therapeutic strategy combining both chemotherapy and/or immunotherapy with an adjuvant treatment targeting the gut microbiota can now be developed in order to improve treatment response and tolerance.
[Mh] Termos MeSH primário: Neoplasias Colorretais/microbiologia
Neoplasias Colorretais/terapia
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Toxinas Bacterianas/metabolismo
Bacteroides fragilis
Proliferação Celular
Resistência a Medicamentos Antineoplásicos
Disbiose/induzido quimicamente
Disbiose/complicações
Enterococcus faecalis
Escherichia coli
Faecalibacterium prausnitzii
Transplante de Microbiota Fecal
Fusobacterium nucleatum
Microbioma Gastrointestinal/fisiologia
Seres Humanos
Neovascularização Patológica/microbiologia
Probióticos/uso terapêutico
Streptococcus gallolyticus
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Toxins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


  8 / 9192 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29253813
[Au] Autor:de Annunzio SR; de Freitas LM; Blanco AL; da Costa MM; Carmona-Vargas CC; de Oliveira KT; Fontana CR
[Ad] Endereço:Universidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquara. Rodovia Araraquara-Jaú, Km1, Campus Ville, Araraquara, SP, CEP 14800-903, Brazil.
[Ti] Título:Susceptibility of Enterococcus faecalis and Propionibacterium acnes to antimicrobial photodynamic therapy.
[So] Source:J Photochem Photobiol B;178:545-550, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Bacterial resistance to available antibiotics nowadays is a global threat leading researchers around the world to study new treatment modalities for infections. Antimicrobial photodynamic therapy (aPDT) has been considered an effective and promising therapeutic alternative in this scenario. Briefly, this therapy is based on the activation of a non-toxic photosensitizing agent, known as photosensitizer (PS), by light at a specific wavelength generating cytotoxic singlet oxygen and free radicals. Virtually all studies related to aPDT involve a huge screening to identify ideal PS concentration and light dose combinations, a laborious and time-consuming process that is hardly disclosed in the literature. Herein, we describe an antimicrobial Photodynamic Therapy (aPDT) study against Enterococcus faecalis and Propionibacterium acnes employing methylene blue, chlorin-e6 or curcumin as PS. Similarities and discrepancies between the two bacterial species were pointed out in an attempt to speed up and facilitate futures studies against those clinical relevant strains. Susceptibility tests were performed by the broth microdilution method. Our results demonstrate that aPDT mediated by the three above-mentioned PS was effective in eliminating both gram-positive bacteria, although P. acnes showed remarkably higher susceptibility to aPDT when compared to E. faecalis. PS uptake assays revealed that P. acnes is 80 times more efficient than E. faecalis in internalizing all three PS molecules. Our results evidence that the cell wall structure is not a limiting feature when predicting bacterial susceptibility to aPDT treatment.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Enterococcus faecalis/efeitos dos fármacos
Fármacos Fotossensibilizantes/farmacologia
Propionibacterium acnes/efeitos dos fármacos
[Mh] Termos MeSH secundário: Anti-Infecciosos/química
Curcumina/química
Curcumina/farmacologia
Enterococcus faecalis/efeitos da radiação
Luz
Azul de Metileno/química
Azul de Metileno/farmacologia
Fármacos Fotossensibilizantes/química
Porfirinas/química
Porfirinas/farmacologia
Propionibacterium acnes/efeitos da radiação
Oxigênio Singlete/química
Oxigênio Singlete/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Photosensitizing Agents); 0 (Porphyrins); 17778-80-2 (Singlet Oxygen); 5S2CCF3T1Z (phytochlorin); IT942ZTH98 (Curcumin); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  9 / 9192 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29175602
[Au] Autor:Thangaraj M; Gengan RM; Ranjan B; Muthusamy R
[Ad] Endereço:Department of Chemistry, Faculty of Applied Sciences, Durban University of Technology, Durban 4001, South Africa.
[Ti] Título:Synthesis, molecular docking, antimicrobial, antioxidant and toxicity assessment of quinoline peptides.
[So] Source:J Photochem Photobiol B;178:287-295, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A series of quinoline based peptides were synthesized by a one-pot reaction through Ugi-four component condensation of lipoic acid, cyclohexyl isocyanide, aniline derivatives and 2-methoxy quinoline-3-carbaldehyde derivatives under microwave irradiation. The products were obtained in excellent yields and high purity. Solvent optimization and the effect of microwave irradiation with various powers were also observed. All the synthesized compounds were characterized by FTIR, NMR spectral data and elemental analysis. A total of eight peptides were subjected to antimicrobial, antioxidant and toxicity evaluation. Among them, four peptides showed potential towards antibacterial screening with Bacillus cereus, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis and Candida albicans, Candida utilis and three peptides showed antioxidant test positive (DPPH). Besides, toxicity of all the peptides were evaluated by using brine shrimp and it was observed that four peptides showed mortality rate less than 50% up to 48h. Molecular docking studies revealed that the higher binding affinity of the two peptides toward DNA gyrase than ciprofloxacin based on Libdock score. The described chemistry represents a facile tool to synthesize complex heterocycles of pharmaceutical relevance in a highly efficient and one-pot fashion. The advantages of this method are its green approach, inexpensive solvent, shorter reaction times and excellent yields.
[Mh] Termos MeSH primário: Anti-Infecciosos/síntese química
Antioxidantes/química
Simulação de Acoplamento Molecular
Peptídeos/química
Quinolinas/química
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Artemia/efeitos dos fármacos
Artemia/crescimento & desenvolvimento
Bacillus cereus/efeitos dos fármacos
Sítios de Ligação
Candida/efeitos dos fármacos
DNA Girase/química
DNA Girase/metabolismo
Enterococcus faecalis/efeitos dos fármacos
Escherichia coli/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Peptídeos/síntese química
Estrutura Terciária de Proteína
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Peptides); 0 (Quinolines); E66400VT9R (quinoline); EC 5.99.1.3 (DNA Gyrase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  10 / 9192 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29325735
[Au] Autor:Hua R; Xia Y; Wu W; Yan J; Yang M
[Ad] Endereço:The First Affiliated Hospital of Chongqing Medical University, Department of Clinical Laboratory, Chongqing 400016, China.
[Ti] Título:Whole transcriptome analysis reveals potential novel mechanisms of low-level linezolid resistance in Enterococcus faecalis.
[So] Source:Gene;647:143-149, 2018 Mar 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Linezolid is an oxazolidinone antibiotic commonly used to treat serious infections caused by vancomycin-resistant enterococcus. Recently, low-level linezolid resistant Enterococcus faecalis strains have emerged worldwide, but the resistant mechanisms remain undefined. Whole-transcriptome profiling was performed on an E. faecalis strain P10748 with low-level linezolid resistance in comparison with a linezolid-susceptible strain 3138 and the standard control strain ATCC29212. The functions of differentially expressed genes (DEGs) were predicted, with some DEGs potentially involved in drug resistance were validated by PCR and quantitative PCR (qPCR). RNA-Seq on three E. faecalis strains generated 1920 unigenes, with 98% of them assigned to various function groups. A total of 150 DEGs were identified in the linezolid resistant strain P10748 compared to the linezolid susceptible strains 3138 and ATCC29212. Functional analysis indicated a significant transcriptomic shift to membrane transportation and biofilm formation in strain P10748, with three significantly up-regulated DEGs predicted to be associated with drug resistance through active efflux pumps and biofilm formation. The existence of these three DEGs was further confirmed by PCR and qPCR. The significant upregulation of genes associated with efflux pumps and biofilm formation in the linezolid resistant strain suggests their roles in low-level resistance to linezolid in E. faecalis.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana/genética
Enterococcus faecalis/efeitos dos fármacos
Enterococcus faecalis/genética
Linezolida/farmacologia
Transcriptoma/genética
[Mh] Termos MeSH secundário: Perfilação da Expressão Gênica/métodos
Oxazolidinonas/farmacologia
Regulação para Cima/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Oxazolidinones); ISQ9I6J12J (Linezolid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE



página 1 de 920 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde