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Pesquisa : B03.353.750.450.475.559 [Categoria DeCS]
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[PMID]:29073460
[Au] Autor:Scariot MC; Venturelli GL; Prudêncio ES; Arisi ACM
[Ad] Endereço:CAL CCA UFSC, Food Science and Technology Department, Federal University of Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001 Florianópolis, SC, Brazil.
[Ti] Título:Quantification of Lactobacillus paracasei viable cells in probiotic yoghurt by propidium monoazide combined with quantitative PCR.
[So] Source:Int J Food Microbiol;264:1-7, 2018 Jan 02.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Propidium monoazide (PMA) coupled with qPCR has been successfully used for specific quantification of viable bacteria cells in diverse matrices food. The present study aimed to develop PMA-qPCR assay for quantification of Lactobacillus paracasei viable cells in probiotic yoghurt. L. paracasei grown in culture medium was submitted to heat treatment at 60°C for different periods of time and probiotic yoghurt containing L. paracasei were prepared and stored at 4°C for 30days. The viable cells were quantified using qPCR and PMA-qPCR assays targeting tuf gene and also by plate counting. Standard curves were prepared and mean efficiency obtained was 94% and 96% (R >0.98) to L. paracasei in culture medium and probiotic yoghurt stored one day, respectively. The limit of detection (LOD) for both samples was 10 genome copies, corresponding to 32.1pg of DNA. For viable cells quantification, standard curves Cq versus log CFU were plotted using mean CFU by plate counting of L. paracasei grown in culture medium and probiotic yoghurt. Results obtained for L. paracasei heat-treated cells were concordant by PMA-qPCR and plate count, CFU decreased as the heat treatment time increased, while qPCR count remained constant. L. paracasei enumerations obtained by qPCR for probiotic yoghurt stored one day and 30days were higher than enumerations by PMA-qPCR for the same samples. The plate count values were similar to CFU values obtained by PMA-qPCR. These results showed that PMA-qPCR is a powerful approach compared with culture-dependent methods for quantification of L. paracasei viable cells in yoghurt. PMA-qPCR allowed reliable obtained results much faster than plate counting.
[Mh] Termos MeSH primário: Azidas/química
Carga Bacteriana/métodos
Lactobacillus paracasei/crescimento & desenvolvimento
Propídio/análogos & derivados
Reação em Cadeia da Polimerase em Tempo Real/métodos
Iogurte/microbiologia
[Mh] Termos MeSH secundário: DNA Bacteriano/análise
Temperatura Alta
Viabilidade Microbiana
Probióticos/análise
Propídio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azides); 0 (DNA, Bacterial); 0 (propidium monoazide); 36015-30-2 (Propidium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE


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[PMID]:28934296
[Au] Autor:Hu WS; Rajendran P; Tzang BS; Yeh YL; Shen CY; Chen RJ; Ho TJ; Vijaya Padma V; Chen YH; Huang CY
[Ad] Endereço:School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan, ROC.
[Ti] Título:Lactobacillus paracasei GMNL-32 exerts a therapeutic effect on cardiac abnormalities in NZB/W F1 mice.
[So] Source:PLoS One;12(9):e0185098, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Systemic lupus erythematosus (SLE) is a disease that mostly affects women. Accelerated atherosclerosis is a high-risk factor associated with SLE patients. SLE associated with cardiovascular disease is one of the most important causes of death. In this study, we demonstrated that Lactobacillus paracasei GMNL-32 (GMNL-32), a probiotic species, exhibits anti-fibrosis and anti-apoptotic effects on the cardiac tissue of NZB/WF1 mice. Female NZB/W F1 mice, a well-known and commonly used lupus-prone mouse strain, were treated with or without GMNL-32 administration for 12 weeks. Oral administration of GMNL-32 to NZB/WF1 mice significantly increased the ventricular thickness when compared to that of NZB/WF1 mice. Administration of GMNL-32 significantly attenuated the cardiac cell apoptosis that was observed in exacerbate levels in the control NZB/WF1 mice. Further, the cellular morphology that was slightly distorted in the NZB/WF1 was effectively alleviated in the treatment group mice. In addition, GMNL-32 reduced the level of Fas death receptor-related pathway of apoptosis signaling and enhanced anti-apoptotic proteins. These results indicate that GMNL-32 exhibit an effective protective effect on cardiac cells of SLE mice. Thus, GMNL-32 may be a potential therapeutic strategy against SLE associated arthrosclerosis.
[Mh] Termos MeSH primário: Lactobacillus paracasei
Lúpus Eritematoso Sistêmico/dietoterapia
Probióticos/administração & dosagem
[Mh] Termos MeSH secundário: Administração Oral
Animais
Apoptose/fisiologia
Western Blotting
Colágeno/metabolismo
Ciclo-Oxigenase 2/metabolismo
Modelos Animais de Doenças
Feminino
Fibrose/dietoterapia
Fibrose/metabolismo
Fibrose/patologia
Imunofluorescência
Ventrículos do Coração/metabolismo
Ventrículos do Coração/patologia
Marcação In Situ das Extremidades Cortadas
Lúpus Eritematoso Sistêmico/metabolismo
Lúpus Eritematoso Sistêmico/patologia
Metaloproteinase 9 da Matriz/metabolismo
Camundongos Endogâmicos NZB
Tamanho do Órgão
Distribuição Aleatória
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-34-5 (Collagen); EC 1.14.99.- (Ptgs2 protein, mouse); EC 1.14.99.1 (Cyclooxygenase 2); EC 3.4.24.35 (Matrix Metalloproteinase 9); EC 3.4.24.35 (Mmp9 protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185098


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[PMID]:28931041
[Au] Autor:Belkacem N; Serafini N; Wheeler R; Derrien M; Boucinha L; Couesnon A; Cerf-Bensussan N; Gomperts Boneca I; Di Santo JP; Taha MK; Bourdet-Sicard R
[Ad] Endereço:Institut Pasteur, Invasive Bacterial Infections Unit, Paris, France.
[Ti] Título:Lactobacillus paracasei feeding improves immune control of influenza infection in mice.
[So] Source:PLoS One;12(9):e0184976, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Respiratory tract infections such as flu cause severe morbidity and mortality and are among the leading causes of death in children and adults worldwide. Commensal microbiota is critical for orchestrating tissue homeostasis and immunity in the intestine. Probiotics represent an interesting source of immune modulators and several clinical studies have addressed the potential beneficial effects of probiotics against respiratory infections. Therefore, we have investigated the mechanisms of protection conferred by L. paracasei CNCM I-1518 strain in a mouse model of influenza infection. Notably, local myeloid cells accumulation is generated in the lungs after seven days feeding with L. paracasei prior to viral infection. L. paracasei-fed mice showed reduced susceptibility to the influenza infection, associated with less accumulation of inflammatory cells in the lungs, faster viral clearance and general health improvement. Interestingly, Allobaculum was significantly increased in L. paracasei-fed mice 7 days after influenza infection, even if the gut microbiota composition was not altered overall. L. paracasei-purified peptidoglycan partially recapitulated the protective phenotype observed with the entire bacteria. Collectively, our results demonstrate that oral consumption of L. paracasei CNCM I-1518 modulates lung immunity was associated with an improved control of influenza infection. These results further extend the beneficial role for certain lactobacilli to alleviate the burden of respiratory tract infections.
[Mh] Termos MeSH primário: Imunidade Celular/imunologia
Lactobacillus paracasei/fisiologia
Infecções por Orthomyxoviridae/imunologia
Orthomyxoviridae/imunologia
Probióticos/administração & dosagem
Infecções Respiratórias/imunologia
[Mh] Termos MeSH secundário: Animais
Contagem de Colônia Microbiana
Feminino
Camundongos
Camundongos Endogâmicos BALB C
Infecções por Orthomyxoviridae/microbiologia
Infecções por Orthomyxoviridae/prevenção & controle
Infecções Respiratórias/microbiologia
Infecções Respiratórias/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184976


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[PMID]:28837656
[Au] Autor:Surachat K; Sangket U; Deachamag P; Chotigeat W
[Ad] Endereço:Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
[Ti] Título:In silico analysis of protein toxin and bacteriocins from Lactobacillus paracasei SD1 genome and available online databases.
[So] Source:PLoS One;12(8):e0183548, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lactobacillus paracasei SD1 is a potential probiotic strain due to its ability to survive several conditions in human dental cavities. To ascertain its safety for human use, we therefore performed a comprehensive bioinformatics analysis and characterization of the bacterial protein toxins produced by this strain. We report the complete genome of Lactobacillus paracasei SD1 and its comparison to other Lactobacillus genomes. Additionally, we identify and analyze its protein toxins and antimicrobial proteins using reliable online database resources and establish its phylogenetic relationship with other bacterial genomes. Our investigation suggests that this strain is safe for human use and contains several bacteriocins that confer health benefits to the host. An in silico analysis of protein-protein interactions between the target bacteriocins and the microbial proteins gtfB and luxS of Streptococcus mutans was performed and is discussed here.
[Mh] Termos MeSH primário: Toxinas Bacterianas/metabolismo
Bacteriocinas/metabolismo
Bases de Dados de Proteínas
Lactobacillus paracasei/genética
[Mh] Termos MeSH secundário: Simulação por Computador
Farmacorresistência Bacteriana
Lactobacillus paracasei/classificação
Lactobacillus paracasei/efeitos dos fármacos
Lactobacillus paracasei/metabolismo
Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Bacteriocins); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183548


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[PMID]:28829589
[Au] Autor:Cheng MC; Pan TM
[Ad] Endereço:Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Number 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
[Ti] Título:Glyceryl 1,3-Dipalmitate Produced from Lactobacillus paracasei subspecies. paracasei NTU 101 Inhibits Oxygen-Glucose Deprivation and Reperfusion-Induced Oxidative Stress via Upregulation of Peroxisome Proliferator-Activated Receptor γ in Neuronal SH-SY5Y Cells.
[So] Source:J Agric Food Chem;65(36):7926-7933, 2017 Sep 13.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glyceryl 1,3-dipalmitate (GD) purified from Lactobacillus paracasei subsp. paracasei NTU 101-fermented products has been demonstrated to possess neuroprotective properties. We determined the effect of GD on oxygen-glucose deprivation and reperfusion (OGD/R)-induced SH-SY5Y neuroblastoma cell death. GD ameliorated OGD/R-induced apoptosis by elevating the protein expression of nuclear peroxisome proliferator-activated receptor γ (PPARγ) and nuclear factor erythroid 2-related factor 2 (Nrf2), thereby attenuating reactive oxygen species (ROS) generation. Pretreatment with GD reduced nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) expression from 1.54 ± 0.27 to 0.84 ± 0.46, thereby attenuating the induction of pro-inflammatory mediators, and increased the plasma membrane Ca ATPase (PMCA) levels from 0.81 ± 0.02 to 1.08 ± 0.06, thus reducing the levels of cytosolic Ca ; this also correlated with reduced cell death. We conclude that GD prevents SH-SY5Y cells from injury after OGD/R insult, possibly by modulating oxidative stress and inflammatory response.
[Mh] Termos MeSH primário: Glucose/metabolismo
Lactobacillus paracasei/química
Neurônios/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Oxigênio/metabolismo
Palmitatos/farmacologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Seres Humanos
Lactobacillus paracasei/metabolismo
NF-kappa B/metabolismo
Neurônios/citologia
Neurônios/metabolismo
PPAR gama/genética
PPAR gama/metabolismo
Palmitatos/química
Palmitatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B); 0 (PPAR gamma); 0 (Palmitates); IY9XDZ35W2 (Glucose); S88TT14065 (Oxygen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02728


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[PMID]:28720151
[Au] Autor:Andersen AD; Cilieborg MS; Lauridsen C; Mørkbak AL; Sangild PT
[Ad] Endereço:1Comparative Pediatrics and Nutrition, Faculty of Health and Medical Sciences,University of Copenhagen, DK-1870 Frederiksberg,Denmark.
[Ti] Título:Supplementation with Lactobacillus paracasei or Pediococcus pentosaceus does not prevent diarrhoea in neonatal pigs infected with Escherichia coli F18.
[So] Source:Br J Nutr;118(2):109-120, 2017 Jul.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Infectious diarrhoea is a worldwide problem in newborns. Optimal bacterial colonisation may enhance gut maturation and protect against pathogenic bacteria after birth. We hypothesised that lactic acid bacteria (LAB) administration prevents pathogen-induced diarrhoea in formula-fed newborns. Newborn caesarean-delivered, colostrum-deprived term piglets on parenteral nutrition for the first 15 h, were used as models for sensitive newborn infants. A commercially available probiotic strain, Lactobacillus paracasei F19 (LAP, 2·6×108 colony-forming units (CFU)/kg per d) and a novel LAB isolate, Pediococcus pentosaceus (PEP, 1·3×1010 CFU/kg per d), were administered for 5 d with or without inoculation of the porcine pathogen, Escherichia coli F18 (F18, 1010 CFU/d). This resulted in six treatment groups: Controls (n 9), LAP (n 10), PEP (n 10), F18 (n 10), F18-LAP (n 10) and F18-PEP (n 10). The pathogen challenge increased diarrhoea and density of F18 in the intestinal mucosa (P<0·05). LAB supplementation further increased the diarrhoea score, relative to F18 alone (P<0·01). Intestinal structure and permeability were similar among groups, whereas brush border enzymes were affected in variable intestinal regions with decreased activities in most cases after F18 and LAB inoculation. Bacterial density in colon mucosa increased after F18 inoculation (P<0·05) but was unaffected by LAB supplementation. In colon contents, acetic and butyric acids were increased by PEP (P<0·05). The LAB used in this study failed to reduce E. coli-induced diarrhoea in sensitive newborn pigs. In vulnerable newborns there may be a delicate balance among bacterial composition and load, diet and the host. Caution may be required when administering LAB to compromised newborns suffering from enteric infections.
[Mh] Termos MeSH primário: Animais Recém-Nascidos/microbiologia
Diarreia/veterinária
Infecções por Escherichia coli/veterinária
Lactobacillus paracasei
Pediococcus pentosaceus
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Ácido Acético/análise
Animais
Ácido Butírico/análise
Colo/química
Colo/microbiologia
Contagem de Colônia Microbiana
Diarreia/microbiologia
Diarreia/prevenção & controle
Dieta/veterinária
Suplementos Nutricionais
Modelos Animais de Doenças
Escherichia coli/crescimento & desenvolvimento
Escherichia coli/isolamento & purificação
Infecções por Escherichia coli/complicações
Mucosa Intestinal/microbiologia
Intestinos/microbiologia
Probióticos/uso terapêutico
Sus scrofa
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
107-92-6 (Butyric Acid); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.1017/S000711451700160X


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[PMID]:28502277
[Au] Autor:Hsu TC; Huang CY; Liu CH; Hsu KC; Chen YH; Tzang BS
[Ad] Endereço:1Institute of Biochemistry, Microbiology and Immunology,Chung Shan Medical University,Taichung 402,Taiwan, ROC.
[Ti] Título:Lactobacillus paracasei GMNL-32, Lactobacillus reuteri GMNL-89 and L. reuteri GMNL-263 ameliorate hepatic injuries in lupus-prone mice.
[So] Source:Br J Nutr;117(8):1066-1074, 2017 Apr.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Probiotics are known to regulate host immunity by interacting with systemic and mucosal immune cells as well as intestinal epithelial cells. Supplementation with certain probiotics has been reported to be effective against various disorders, including immune-related diseases. However, little is known about the effectiveness of Lactobacillus paracasei GMNL-32 (GMNL-32), Lactobacillus reuteri GMNL-89 (GMNL-89) and L. reuteri GMNL-263 (GMNL-263) in the management of autoimmune diseases, especially systemic lupus erythematosus (SLE). NZB/W F1 mice, which are a lupus-prone animal model, were orally gavaged with GMNL-32, GMNL-89 or GMNL-263 to investigate the effects of these Lactobacillus strains on liver injuries in NZB/W F1 mice. The results thus obtained reveal that supplementary GMNL-32, GMNL-89 or GMNL-263 in NZB/W F1 mice ameliorates hepatic apoptosis and inflammatory indicators, such as matrix metalloproteinase-9 activity and C-reactive protein and inducible nitric oxide synthase expressions. In addition, supplementation with GMNL-32, GMNL-89 or GMNL-263 in NZB/W F1 mice reduced the expressions of hepatic IL-1ß, IL-6 and TNF-α proteins by suppressing the mitogen-activated protein kinase and NF-κB signalling pathways. These findings, presented here for the first time, reveal that GMNL-32, GMNL-89 and GMNL-263 mitigate hepatic inflammation and apoptosis in lupus-prone mice and may support an alternative remedy for liver disorders in cases of SLE.
[Mh] Termos MeSH primário: Lactobacillus paracasei/classificação
Hepatopatias/etiologia
Lúpus Eritematoso Sistêmico/complicações
[Mh] Termos MeSH secundário: Animais
Apoptose
Proteína C-Reativa/metabolismo
Citocinas/genética
Citocinas/metabolismo
Feminino
Regulação da Expressão Gênica/fisiologia
Hepatócitos/microbiologia
Hepatócitos/fisiologia
Lactobacillus reuteri
Hepatopatias/prevenção & controle
Metaloproteinase 9 da Matriz/genética
Metaloproteinase 9 da Matriz/metabolismo
Camundongos
Camundongos Endogâmicos NZB
Óxido Nítrico Sintase/genética
Óxido Nítrico Sintase/metabolismo
Probióticos
Distribuição Aleatória
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 9007-41-4 (C-Reactive Protein); EC 1.14.13.39 (Nitric Oxide Synthase); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517001039


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[PMID]:28400010
[Au] Autor:Campanella D; Rizzello CG; Fasciano C; Gambacorta G; Pinto D; Marzani B; Scarano N; De Angelis M; Gobbetti M
[Ad] Endereço:Department of Soil, Plant and Food Science, Via G. Amendola 165/a, 70126, University of Bari Aldo Moro, Bari, Italy.
[Ti] Título:Exploitation of grape marc as functional substrate for lactic acid bacteria and bifidobacteria growth and enhanced antioxidant activity.
[So] Source:Food Microbiol;65:25-35, 2017 Aug.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study aimed at using grape marc for the growth of lactic acid bacteria and bifidobacteria with the perspective of producing a functional ingredient having antioxidant activity. Lactobacillus plantarum 12A and PU1, Lactobacillus paracasei 14A, and Bifidobacterium breve 15A showed the ability to grow on grape marc (GM) based media. The highest bacterial cell density (>9.0 CFU/g) was found in GM added of 1% of glucose (GMG). Compared to un-inoculated and incubated control fermented GMG showed a decrease of carbohydrates and citric acid together with an increase of lactic acid. The content of several free amino acids and phenol compounds differed between samples. Based on the survival under simulated gastro-intestinal conditions, GMG was a suitable carrier of lactic acid bacteria and bifidobacteria strains. Compared to the control, cell-free supernatant (CFS) of fermented GMG exhibited a marked antioxidant activity in vitro. The increased antioxidant activity was confirmed using Caco-2 cell line after inducing oxidative stress, and determining cell viability and radical scavenging activity through MTT and DCFH-DA assays, respectively. Supporting these founding, the SOD-2 gene expression of Caco-2 cells also showed a lowest pro-oxidant effect induced by the four CFS of GMG fermented by lactic acid bacteria and bifidobacteria.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Bifidobacterium/crescimento & desenvolvimento
Ácido Láctico/metabolismo
Lactobacillus paracasei/crescimento & desenvolvimento
Lactobacillus plantarum/crescimento & desenvolvimento
Vitis/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/análise
Antioxidantes/farmacologia
Bifidobacterium/metabolismo
Células CACO-2
Sobrevivência Celular/efeitos dos fármacos
Meios de Cultura/farmacologia
Fermentação
Ácido Gástrico/metabolismo
Glucose/metabolismo
Seres Humanos
Lactobacillus paracasei/metabolismo
Lactobacillus plantarum/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Fenóis/análise
Superóxido Dismutase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Antioxidants); 0 (Culture Media); 0 (Phenols); 33X04XA5AT (Lactic Acid); EC 1.15.1.1 (Superoxide Dismutase); EC 1.15.1.1 (superoxide dismutase 2); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170427
[Lr] Data última revisão:
170427
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE


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[PMID]:28361716
[Au] Autor:Tada I; Tanizawa Y; Arita M
[Ad] Endereço:Center for Information Biology, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan.
[Ti] Título:Visualization of consensus genome structure without using a reference genome.
[So] Source:BMC Genomics;18(Suppl 2):208, 2017 03 14.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Standard graphical tools for whole genome comparison require a reference genome. However, any reference is also subject to annotation biases and rearrangements, and may not serve as the standard except for those of extensively studied model species. To fully exploit the rapidly accumulating sequence data from the recent sequencing technologies, genome comparison without any reference has been anticipated. RESULTS: We introduce a circular genome visualizer to compare complete genomes of closely related species. This tool visualizes the position of orthologous gene clusters rather than actual sequences or their features, thereby achieving the comparative view without using a single reference genome. The essential information is the matrix of orthologous gene clusters whose positions (not sequences) are color-coded in circular graphics. As a demonstration, comparison of 14 Lactobacillus paracasei strains and one L. casei strain revealed not only large-scale rearrangements but also genomic islands that are strain-specific. Comparison of 73 Helicobacter pylori strains confirmed their genetic consistency and also revealed the three general patterns of large-scale genome inversions. CONCLUSIONS: From the ample sequence information in the GenBank/ENA/DDBJ repository, we can reconstruct a genomic consensus for particular species. By visualizing multiple strains at a glance, we can identify conserved as well as strain-specific regions in multiply sequenced genomes. Positional consistency for orthologous genes provides information orthogonal to major sequence features such as the GC content or sequence similarity of marker genes. The positional comparison is therefore useful for identifying large-scale genome rearrangements or gene transfers.
[Mh] Termos MeSH primário: Mapeamento Cromossômico/métodos
Genoma Bacteriano
Helicobacter pylori/genética
Lactobacillus paracasei/genética
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Composição de Bases
Gráficos por Computador
Bases de Dados de Ácidos Nucleicos
Rearranjo Gênico
Ilhas Genômicas
Helicobacter pylori/classificação
Lactobacillus paracasei/classificação
Família Multigênica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-017-3499-7


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[PMID]:28267809
[Au] Autor:Rossoni RD; Fuchs BB; de Barros PP; Velloso MD; Jorge AO; Junqueira JC; Mylonakis E
[Ad] Endereço:Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.
[Ti] Título:Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection.
[So] Source:PLoS One;12(3):e0173332, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Probiotics have been described as a potential strategy to control opportunistic infections due to their ability to stimulate the immune system. Using the non-vertebrate model host Galleria mellonella, we evaluated whether clinical isolates of Lactobacillus spp. are able to provide protection against Candida albicans infection. Among different strains of Lactobacillus paracasei, Lactobacillus rhamnosus and Lactobacillus fermentum, we verified that L. paracasei 28.4 strain had the greatest ability to prolong the survival of larvae infected with a lethal dose of C. albicans. We found that the injection of 107 cells/larvae of L. paracasei into G. mellonella larvae infected by C. albicans increased the survival of these insects compared to the control group (P = 0.0001). After that, we investigated the immune mechanisms involved in the protection against C. albicans infection, evaluating the number of hemocytes and the gene expression of antifungal peptides. We found that L. paracasei increased the hemocyte quantity (2.38 x 106 cells/mL) in relation to the control group (1.29 x 106 cells/mL), indicating that this strain is capable of raising the number of circulating hemocytes into the G. mellonella hemolymph. Further, we found that L. paracasei 28.4 upregulated genes that encode the antifungal peptides galiomicin and gallerymicin. In relation to the control group, L. paracasei 28.4 increased gene expression of galiomicin by 6.67-fold and 17.29-fold for gallerymicin. Finally, we verified that the prophylactic provision of probiotic led to a significant reduction of the number of fungal cells in G. mellonella hemolymph. In conclusion, L. paracasei 28.4 can modulate the immune system of G. mellonella and protect against candidiasis.
[Mh] Termos MeSH primário: Candida albicans/imunologia
Resistência à Doença/imunologia
Imunomodulação
Lactobacillus paracasei/fisiologia
Mariposas/imunologia
Mariposas/microbiologia
[Mh] Termos MeSH secundário: Animais
Contagem de Colônia Microbiana
Regulação da Expressão Gênica
Hemócitos
Hemolinfa
Interações Hospedeiro-Patógeno/genética
Interações Hospedeiro-Patógeno/imunologia
Seres Humanos
Larva/microbiologia
Mariposas/genética
Probióticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173332



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