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[PMID]:29458538
[Au] Autor:Ma J; Yu L; Song B; Yu Y; Zhang S; Wei Y; Wu Z; Yao D; Yu W; Zhu Z; Cui Y
[Ad] Endereço:1​College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China.
[Ti] Título:The double adjuvants LTB and CpG significantly enhanced the immuno-protective effects of recombinant GIT derived from Staphylococcus aureus and Streptococcus in mice.
[So] Source:J Med Microbiol;67(3):432-440, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: In this study, we prepared GapC1-150-IsdB126-361-TRAP (GIT) proteins plus heat-labile enterotoxin B (LTB) as an intra-molecular adjuvant, together with CpG to further enhance its immunogenicity. METHODOLOGY: Initially, the target genes were acquired and inserted into pET-32a (+) vectors to express LTB-GIT protein. LTB-GIT expression was confirmed by Western blotting and its immunocompetence was estimated through ELISA. Further, we immunized BALB/c mice with the LTB-GIT plus CpG adjuvant. After the second immunization, the antigen-specific CD4 cell responses for IFN-γ, IL-2, IL-4 and IL-10 were monitored by intracellular cytokine staining (ICS) assay. After the third immunization, the level of IgG antibodies in the serum from immunized groups was assessed by ELISA, and the protective immune response was appraised by Staphylococcus aureus and Streptococcus dysgalactiae challenge. RESULTS: The ELISA results showed that the OD450nm value of the LTB-GIT group was significantly higher than that of the BSA group. The group immunized with LTB-GIT plus CpG exhibited significantly stronger CD4 T cell responses for IFN-γ, IL-2, IL-4 and IL-10 compared to the group immunized with LTB-GIT, GIT alone orLTB-GIT plus CpG. In addition, the group immunized with LTB-GIT plus CpG generated the highest level of IgG antibodies against GIT among all of the groups, and our results also showed that LTB-GIT plus CpG markedly improved the survival percentage of mice compared to other groups. CONCLUSION: We confirmed that the novel double adjuvants, LTB and CpG, are able to significantly improve GIT-induced immune responses. This formula could be a promising strategy for enhancing the immune efficacy of multi-subunit vaccines against Staphylococcus aureus and streptococcal infection.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos
Toxinas Bacterianas/imunologia
Vacinas Bacterianas/imunologia
Enterotoxinas/imunologia
Oligodesoxirribonucleotídeos/imunologia
Staphylococcus aureus/imunologia
Streptococcus/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Proteínas de Bactérias/imunologia
Toxinas Bacterianas/administração & dosagem
Toxinas Bacterianas/genética
Linfócitos T CD4-Positivos
Enterotoxinas/administração & dosagem
Enterotoxinas/genética
Feminino
Interferon gama/imunologia
Interleucina-10/imunologia
Interleucina-2/imunologia
Interleucina-4/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Oligodesoxirribonucleotídeos/genética
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Infecções Estafilocócicas/imunologia
Staphylococcus aureus/química
Infecções Estreptocócicas/imunologia
Streptococcus/química
Vacinação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (Bacterial Vaccines); 0 (CPG-oligonucleotide); 0 (Enterotoxins); 0 (IL10 protein, mouse); 0 (Interleukin-2); 0 (Oligodeoxyribonucleotides); 0 (Recombinant Proteins); 130068-27-8 (Interleukin-10); 207137-56-2 (Interleukin-4); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000666


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[PMID]:28449969
[Au] Autor:Le Doare K; Faal A; Jaiteh M; Sarfo F; Taylor S; Warburton F; Humphries H; Birt J; Jarju S; Darboe S; Clarke E; Antonio M; Foster-Nyarko E; Heath PT; Gorringe A; Kampmann B
[Ad] Endereço:Centre for International Child Health, Imperial College London, Norfolk Place, London W2 1PG, UK,; Paediatric Infectious Diseases Research Group, St. George's University of London, Cranmer Terrace, London SW17 0TE, UK; Vaccines & Immunity Theme, MRC Unit The Gambia, Atlantic Road, Fajara, Gambia
[Ti] Título:Association between functional antibody against Group B Streptococcus and maternal and infant colonization in a Gambian cohort.
[So] Source:Vaccine;35(22):2970-2978, 2017 05 19.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Vertical transmission of Group B Streptococcus (GBS) is a prerequisite for early-onset disease and a consequence of maternal GBS colonization. Disease protection is associated with maternally-derived anti-GBS antibody. Using a novel antibody-mediated C3b/iC3b deposition flow cytometry assay which correlates with opsonic killing we developed a model to assess the impact of maternally-derived functional anti-GBS antibody on infant GBS colonization from birth to day 60-89 of life. METHODS: Rectovaginal swabs and cord blood (birth) and infant nasopharyngeal/rectal swabs (birth, day 6 and day 60-89) were obtained from 750 mother/infant pairs. Antibody-mediated C3b/iC3b deposition with cord and infant sera was measured by flow cytometry. RESULTS: We established that as maternally-derived anti-GBS functional antibody increases, infant colonization decreases at birth and up to three months of life, the critical time window for the development of GBS disease. Further, we observed a serotype (ST)-dependent threshold above which no infant was colonized at birth. Functional antibody above the upper 95th confidence interval for the geometric mean concentration was associated with absence of infant GBS colonization at birth for STII (p<0.001), STIII (p=0.01) and STV (p<0.001). Increased functional antibody was also associated with clearance of GBS between birth and day 60-89. CONCLUSIONS: Higher concentrations of maternally-derived antibody-mediated complement deposition are associated with a decreased risk of GBS colonization in infants up to day 60-89 of life. Our findings are of relevance to establish thresholds for protection following vaccination of pregnant women with future GBS vaccines.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/imunologia
Imunidade Materno-Adquirida
Transmissão Vertical de Doença Infecciosa
Infecções Estreptocócicas/imunologia
Streptococcus/crescimento & desenvolvimento
Streptococcus/imunologia
[Mh] Termos MeSH secundário: Adulto
Anticorpos Antibacterianos/sangue
Portador Sadio
Criança
Pré-Escolar
Estudos de Coortes
Complemento C3b/imunologia
Feminino
Sangue Fetal/imunologia
Citometria de Fluxo
Gâmbia/epidemiologia
Seres Humanos
Técnicas Imunológicas
Lactente
Recém-Nascido
Estudos Longitudinais
Mães
Nasofaringe/microbiologia
Proteínas Opsonizantes
Gravidez
Complicações Infecciosas na Gravidez/microbiologia
Infecções Estreptocócicas/epidemiologia
Infecções Estreptocócicas/transmissão
Streptococcus/classificação
Streptococcus/isolamento & purificação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Opsonin Proteins); 80295-43-8 (Complement C3b)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


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[PMID]:27774436
[Au] Autor:Huang J; Ma J; Shang K; Hu X; Liang Y; Li D; Wu Z; Dai L; Chen L; Wang L
[Ad] Endereço:Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
[Ti] Título:Evolution and Diversity of the Antimicrobial Resistance Associated Mobilome in : A Probable Mobile Genetic Elements Reservoir for Other Streptococci.
[So] Source:Front Cell Infect Microbiol;6:118, 2016.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:is a previously neglected, newly emerging multidrug-resistant zoonotic pathogen. Mobile genetic elements (MGEs) play a key role in intra- and interspecies horizontal transfer of antimicrobial resistance (AMR) determinants. Although, previous studies showed the presence of several MGEs, a comprehensive analysis of AMR-associated mobilome as well as their interaction and evolution has not been performed. In this study, we presented the AMR-associated mobilome and their insertion hotspots in . Integrative conjugative elements (ICEs), prophages and tandem MGEs were located at different insertion sites, while 86% of the AMR-associated MGEs were inserted at and loci. Comprehensive analysis of insertions at and loci among four pathogenic species ( , and ) revealed the existence of different groups of MGEs, including Tn5252, ICE 1108, and TnGBS2 groups ICEs, Φm46.1 group prophage, ICE_ICE and ICE_prophage tandem MGEs. Comparative ICE genomics of ICE 2603 family revealed that module exchange and acquisition/deletion were the main mechanisms in MGEs' expansion and evolution. Furthermore, the observation of tandem MGEs reflected a novel mechanism for MGE diversity. Moreover, an competition assay showed no visible fitness cost was observed between different MGE-carrying isolates and a conjugation assay revealed the transferability of ICE 2603 family of ICEs. Our statistics further indicated that the prevalence and diversity of MGEs in is much greater than in other three species which prompted our hypothesis that is probably a MGEs reservoir for other streptococci. In conclusion, our results showed that acquisition of MGEs confers not only its capability as a multidrug resistance pathogen, but also represents a paradigm to study the modular evolution and matryoshkas of MGEs.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana
Sequências Repetitivas Dispersas
Streptococcus/efeitos dos fármacos
Streptococcus/genética
[Mh] Termos MeSH secundário: Conjugação Genética
Transferência Genética Horizontal
Loci Gênicos
Recombinação Genética
Análise de Sequência de DNA
Streptococcus/crescimento & desenvolvimento
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171224
[Lr] Data última revisão:
171224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29191329
[Au] Autor:Olmos C; Vilacosta I; Fernández-Pérez C; Bernal JL; Ferrera C; García-Arribas D; Pérez-García CN; San Román JA; Maroto L; Macaya C; Elola FJ
[Ad] Endereço:Cardiovascular Institute, Hospital Clínico San Carlos, Madrid, Spain. Electronic address: carmen.olmosblanco@gmail.com.
[Ti] Título:The Evolving Nature of Infective Endocarditis in Spain: A Population-Based Study (2003 to 2014).
[So] Source:J Am Coll Cardiol;70(22):2795-2804, 2017 Dec 05.
[Is] ISSN:1558-3597
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Little information exists regarding population-based epidemiological changes in infective endocarditis (IE) in Europe. OBJECTIVES: This study sought to analyze temporal trends in IE in Spain from 2003 to 2014. METHODS: This retrospective, population-based, temporal trend study analyzed the incidence, epidemiological and clinical characteristics, and outcome of all patients discharged from hospitals included in the Spanish National Health System with a diagnosis of IE, from January 2003 to December 2014. RESULTS: Overall, 16,867 episodes of IE were identified during the study period, 66.3% in men. The rate of IE significantly increased, from 2.72 in 2003 to 3.49 per 100,000 person-years in 2014, and this rise was higher among older adults. The most frequent microorganisms were staphylococci (28.7%), followed by streptococci (20.4%) and enterococci (13.1%). Twenty-three percent of patients underwent cardiac surgery. The in-hospital mortality rate was 20.4%. Throughout the study period, the proportion of patients with previously known heart valve disease and diabetes mellitus significantly increased, whereas the prevalence of intravenous drug use decreased. Regarding microorganisms, Staphylococcus aureus and streptococci slightly declined, whereas coagulase-negative staphylococci and enterococci consistently increased over the years. In-hospital complications and cardiac surgery rates significantly increased across the years. The risk-adjusted in-hospital mortality rate diminished (0.2% per year) during the study period. CONCLUSIONS: The incidence of IE episodes significantly increased over the decade of the study period, particularly among older adults. Relevant changes in clinical and microbiological profile included older patients with more comorbidity and a rise in enterococci and coagulase-negative staphylococcal infections. Adjusted mortality rates slightly declined over the study period.
[Mh] Termos MeSH primário: Procedimentos Cirúrgicos Cardíacos
Endocardite
Enterococcus/isolamento & purificação
Doenças das Valvas Cardíacas
Streptococcus/isolamento & purificação
[Mh] Termos MeSH secundário: Adulto
Idoso
Procedimentos Cirúrgicos Cardíacos/métodos
Procedimentos Cirúrgicos Cardíacos/estatística & dados numéricos
Procedimentos Cirúrgicos Cardíacos/tendências
Endocardite/diagnóstico
Endocardite/microbiologia
Endocardite/mortalidade
Endocardite/cirurgia
Feminino
Doenças das Valvas Cardíacas/diagnóstico
Doenças das Valvas Cardíacas/epidemiologia
Doenças das Valvas Cardíacas/etiologia
Mortalidade Hospitalar/tendências
Seres Humanos
Incidência
Masculino
Meia-Idade
Estudos Retrospectivos
Espanha/epidemiologia
Análise Espaço-Temporal
Staphylococcus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171205
[Lr] Data última revisão:
171205
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE


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[PMID]:28456490
[Au] Autor:Shinjoh M; Yamaguchi Y; Iwata S
[Ad] Endereço:Department of Pediatrics, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; Center for Infectious Diseases and Infection Control, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. Electronic address: m-shinjo@z2.keio.jp.
[Ti] Título:Pediatric bacterial meningitis in Japan, 2013-2015 - 3-5 years after the wide use of Haemophilus influenzae type b and Streptococcus pneumoniae conjugated vaccines.
[So] Source:J Infect Chemother;23(7):427-438, 2017 Jul.
[Is] ISSN:1437-7780
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Haemophilus influenzae type b (Hib) vaccine and pneumococcal conjugated vaccine (PCV) have been widely used since 2010 in Japan when both vaccines were supported by the regional governments, and they were covered as routine recommended vaccines in 2013. The incidence of bacterial meningitis due to these organisms decreased in 2011 and 2012, but meningitis due to Streptococcus agalactiae and Escherichia coli remained unchanged. OBJECTIVES: We planned to confirm whether the incidence also decreased in subsequent years. METHODS: We analyzed the epidemiological and clinical data for 2013-2015, and compared the information obtained in the previous nationwide survey database and our previous reports. We also investigated the risk factors for disease outcome. RESULTS: In the 2013-2015 surveys, 407 patients from 366 hospitals from all prefectures were evaluated. S. agalactiae (33%), Streptococcus pneumoniae (25%), and E. coli (10%) were the main organisms. The total number of patients hospitalized with bacterial meningitis per 1000 admissions decreased from 1.19 in 2009-2010 to 0.37 in 2013-2015 (p < 0.001). The incidence of H. influenzae and S. pneumoniae meningitis significantly decreased from 0.66 in 2009-2010 to 0.01 in 2013-2015, and from 0.30 to 0.09, respectively (p < 0.001). Only 0-2 cases with Neisseria meningitidis were reported each year throughout 2001-2015. The fatality rates for H. influenzae, S. pneumoniae, S. agalactiae, and E. coli in 2013-2015 were 0.0, 4.1, 3.1, and 2.6%, respectively. Risk factors for death and sequelae were consciousness disturbance, convulsion, low CSF glucose, and Staphylococcus sp. as a causative organism (p < 0.01). CONCLUSIONS: Hib vaccine and PCV have decreased the rate of bacterial meningitis. S. agalactiae has subsequently become the most common cause of bacterial meningitis in Japan.
[Mh] Termos MeSH primário: Cápsulas Bacterianas
Vacinas Anti-Haemophilus
Meningites Bacterianas/epidemiologia
Meningites Bacterianas/microbiologia
Vacinas Pneumocócicas
[Mh] Termos MeSH secundário: Adolescente
Anti-Inflamatórios/uso terapêutico
Criança
Pré-Escolar
Estudos de Coortes
Estudos Transversais
Dexametasona/uso terapêutico
Feminino
Haemophilus influenzae
Seres Humanos
Lactente
Recém-Nascido
Japão/epidemiologia
Masculino
Meningites Bacterianas/prevenção & controle
Fatores de Risco
Streptococcus
Vacinação
Vacinas Conjugadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Haemophilus Vaccines); 0 (Haemophilus influenzae type b polysaccharide vaccine); 0 (Pneumococcal Vaccines); 0 (Vaccines, Conjugate); 7S5I7G3JQL (Dexamethasone)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:27777193
[Au] Autor:Hu XY; Yao YF; Cui BM; Lv J; Shen X; Ren B; Li MY; Guo Q; Huang RJ; Li Y
[Ad] Endereço:State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China. E-mail: drhuxiaoyu@163.com.
[Ti] Título:[Analysis of causes and whole microbial structure in a case of rampant caries].
[So] Source:Nan Fang Yi Ke Da Xue Xue Bao;36(10):1328-1333, 2016 Oct 20.
[Is] ISSN:1673-4254
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To analyze the whole microbial structure in a case of rampant caries to provide evidence for its prevention and treatment. METHODS: Clinical samples including blood, supragingival plaque, plaque in the caries cavity, saliva, and mucosal swabs were collected with the patient's consent. The blood sample was sent for routine immune test, and the others samples were stained using Gram method and cultured for identifying colonies and 16S rRNA sequencing. DNA was extracted from the samples and tested for the main cariogenic bacterium (Streptococcus mutans) with qPCR, and the whole microbial structure was analyzed using DGGE. RESULTS: The patient had a high levels of IgE and segmented neutrophils in his blood. Streptococci with extremely long chains were found in the saliva samples under microscope. Culture of the samples revealed the highest bacterial concentration in the saliva. The relative content of hemolytic bacterium was detected in the samples, the highest in the caries cavity; C. albicans was the highest in the dental plaque. In addition, 33 bacterial colonies were identified by VITEK system and 16S rDNA sequence phylogenetic analysis, and among them streptococci and Leptotrichia wade were enriched in the dental plaque sample, Streptococcus mutans, Fusobacterium nucleatum, and Streptococcus tigurinus in the caries cavity, and Lactobacillus in the saliva. S. mutans was significantly abundant in the mucosal swabs, saliva and plaque samples of the caries cavity as shown by qPCR. Compared to samples collected from a healthy individual and another two patients with rampant caries, the samples from this case showed a decreased bacterial diversity and increased bacterial abundance shown by PCR-DGGE profiling, and multiple Leptotrichia sp. were detected by gel sequencing. CONCLUSION: The outgrowth of such pathogenic microorganisms as S. mutans and Leptotrichia sp., and dysbiosis of oral microbial community might contribute to the pathogenesis of rampant caries in this case.
[Mh] Termos MeSH primário: Cárie Dentária/microbiologia
Microbiota
[Mh] Termos MeSH secundário: Anormalidades Múltiplas
Placa Dentária/microbiologia
Fusobacterium/isolamento & purificação
Seres Humanos
Imunoglobulina E/sangue
Lactobacillus/isolamento & purificação
Leptotrichia/isolamento & purificação
Deformidades Congênitas dos Membros
Mucosa Bucal/microbiologia
Neutrófilos/citologia
Filogenia
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Saliva/microbiologia
Streptococcus/isolamento & purificação
Anormalidades Dentárias
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:29016613
[Au] Autor:Bourgeois D; David A; Inquimbert C; Tramini P; Molinari N; Carrouel F
[Ad] Endereço:Laboratory "Systemic Health Care" EA4129, University Lyon 1, Lyon, France.
[Ti] Título:Quantification of carious pathogens in the interdental microbiota of young caries-free adults.
[So] Source:PLoS One;12(10):e0185804, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The majority of caries lesions in adults occur on the proximal tooth surfaces of the posterior teeth. A comprehensive study of the composition of the oral microbiota is fundamental for a better understanding of the etiology of interdental caries. METHODS: Twenty-five caries-free subjects (20-35 years old) were enrolled in the study. The interdental biofilm of four interdental sites were collected. The real-time polymerase chain reaction (PCR) methodology were used to quantify (i) the following bacteria: Streptococcus spp., Streptococcus mutans, Lactobacillus spp., Enterococcus spp., and Enterococcus faecalis; (ii) the fungus Candida albicans; and (iii) total bacteria. RESULTS: Streptococcus spp. was the most abundant species, followed by Lactobacillus spp. and Enterococcus spp. Streptococcus spp. and Lactobacillus spp. were detected at all tested sites and Enterococcus spp. at 99% of sites. S. mutans was detected at only 28% of the tested sites and C. albicans was detected at 11% of sites. E. faecalis was never detected. In 54.5% of the biofilm inhabited by C. albicans, S. mutans was present. Moreover, 28% of the ID sites co-expressed S. mutans and Lactobacillus spp. The studied pathogens were organized into two correlated groups of species. Strikingly, the fungus C. albicans and the bacteria Enterococcus spp. cluster together, whereas Streptococcus spp., S. mutans and Lactobacillus spp. form one distinct cluster. CONCLUSION: The interdental biofilm of young caries-free adults is comprised of pathogens that are able to induce interproximal caries. That several of these pathogens are implicated in heart disease or other systemic diseases is an argument for the disruption of interdental biofilms using daily oral hygiene.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Candida albicans/isolamento & purificação
Placa Dentária/microbiologia
Enterococcus/isolamento & purificação
Lactobacillus/isolamento & purificação
Streptococcus/isolamento & purificação
[Mh] Termos MeSH secundário: Adulto
Carga Bacteriana
Candida albicans/classificação
Candida albicans/genética
DNA Bacteriano/genética
Enterococcus/classificação
Enterococcus/genética
Feminino
Seres Humanos
Lactobacillus/classificação
Lactobacillus/genética
Masculino
Microbiota/genética
Higiene Bucal/educação
Reação em Cadeia da Polimerase em Tempo Real
Streptococcus/classificação
Streptococcus/genética
Dente/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185804


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[PMID]:28885723
[Au] Autor:Ma JS; Chen SY; Lo HH
[Ad] Endereço:Department of Pediatrics, Feng-Yuan Hospital, Taichung, Taiwan.
[Ti] Título:Biofilm formation of beta-hemolytic group G Streptococcus dysgalactiae subspecies equisimilis isolates and its association with emm polymorphism.
[So] Source:APMIS;125(11):1027-1032, 2017 Nov.
[Is] ISSN:1600-0463
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Biofilm formation has been well known as a determinant of bacterial virulence. Group G Streptococcus dysgalactiae subspecies equisimilis (SDSE), a relevant pathogen with increasing medical importance, was evaluated for the biofilm-forming potential. Microtiter plate assay was used to assess the most feasible medium for group G SDSE to form a biofilm. Among 246 SDSE isolates examined, 46.7%, 43.5%, 33.3%, and 26.4% of isolates showed moderate or strong biofilm-forming abilities using tryptic soy broth (TSB), brain heart infusion broth (BHI), Todd-Hewitt broth (THB), and C medium with 30 mM glucose (CMG), respectively. The addition of glucose significantly increased the biofilm-forming ability of group G SDSE. FCT (fibronectin-collagen-T-antigen) typing of SDSE was first undertaken and 11 FCT types were found. Positive associations of stG10.0 or negative associations of stG245.0, stG840.0, and stG6.1 with biofilm-forming ability of SDSE were, respectively, found. This was the first investigation demonstrating biofilm-forming potential in clinical group G SDSE isolates; also, some significant associations of biofilm-forming ability with certain emm types were presented.
[Mh] Termos MeSH primário: Antígenos de Bactérias/genética
Proteínas da Membrana Bacteriana Externa/genética
Biofilmes/efeitos dos fármacos
Proteínas de Transporte/genética
Glucose/farmacologia
Streptococcus/efeitos dos fármacos
Streptococcus/genética
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Biofilmes/crescimento & desenvolvimento
Meios de Cultura/química
Meios de Cultura/farmacologia
Expressão Gênica
Glucose/metabolismo
Seres Humanos
Polimorfismo Genético
Infecções Estreptocócicas/epidemiologia
Infecções Estreptocócicas/microbiologia
Streptococcus/classificação
Streptococcus/isolamento & purificação
Taiwan/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Bacterial Outer Membrane Proteins); 0 (Carrier Proteins); 0 (Culture Media); 0 (streptococcal M protein); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1111/apm.12746


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[PMID]:28802354
[Au] Autor:Kassab A; Dabous O; Morsy M
[Ad] Endereço:Medical Department, National Institute of Laser Enhanced Sciences, Cairo University, Egypt. Electronic address: Kassabahmed28@yahoo.com.
[Ti] Título:A novel management of streptococcal pharyngotonsillar infections by laser-activated silver nanoparticles and methylene blue conjugate, in vitro study.
[So] Source:Int J Pediatr Otorhinolaryngol;100:114-118, 2017 Sep.
[Is] ISSN:1872-8464
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Species of Streptococcus are classified based on their hemolytic properties into alpha and beta types. And, since antimicrobial drug resistance is an increasingly problematic issue, the efforts to develop modalities that would overcome this obstacle and avoid antibiotic side effects is an ongoing challenge. METHODS AND MATERIALS: 20 patients from both sexes were selected. The isolated organisms were identified according to standard laboratory methods. Bacterial Cultures were subjected to the low-level diode laser (660 nm), methylene blue (MB) as a photosensitizing agent and for silver nanoparticles. RESULTS: All the experimental groups showed statistically lower values of CFU/ml than the positive control group. CONCLUSION: The photoactivated MB, silver nanoparticles conjugate showed the maximum inhibitory effect on Streptococci, which opens a gate to further investigation of such a promising protocol to establish a safe and efficient method of management for resistant cases of streptococcal tonsillar infections.
[Mh] Termos MeSH primário: Lasers Semicondutores/uso terapêutico
Azul de Metileno/uso terapêutico
Nanopartículas/uso terapêutico
Fármacos Fotossensibilizantes/uso terapêutico
Infecções Estreptocócicas/tratamento farmacológico
Streptococcus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
Feminino
Seres Humanos
Técnicas In Vitro
Masculino
Prata/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosensitizing Agents); 3M4G523W1G (Silver); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170814
[St] Status:MEDLINE


  10 / 18761 MEDLINE  
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[PMID]:28792235
[Au] Autor:Pummer A; Knüttel H; Hiller KA; Buchalla W; Cieplik F; Maisch T
[Ad] Endereço:Department of Conservative Dentistry & Periodontology, University Medical Center Regensburg, 93053 Regensburg, Germany.
[Ti] Título:Antimicrobial efficacy of irradiation with visible light on oral bacteria in vitro: a systematic review.
[So] Source:Future Med Chem;9(13):1557-1574, 2017 Sep.
[Is] ISSN:1756-8927
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: Resistances to antibiotics employed for treatment of infectious diseases have increased to alarming numbers making it more and more difficult to treat diseases caused by microorganisms resistant to common antibiotics. Consequently, novel methods for successful inactivation of pathogens are required. In this instance, one alternative could be application of light for treatment of topical infections. Antimicrobial properties of UV light are well documented, but due to its DNA-damaging properties use for medical purposes is limited. In contrast, irradiation with visible light may be more promising. METHODS: Literature was systematically screened for research concerning inactivation of main oral bacterial species by means of visible light. RESULTS: Inactivation of bacterial species, especially pigmented ones, in planktonic state showed promising results. There is a lack of research examining the situation when organized as biofilms. CONCLUSION: More research concerning situation in a biofilm state is required.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Bactérias/efeitos dos fármacos
Luz
[Mh] Termos MeSH secundário: Aggregatibacter/efeitos dos fármacos
Aggregatibacter/efeitos da radiação
Anti-Infecciosos/química
Bactérias/efeitos da radiação
Escherichia coli/efeitos dos fármacos
Escherichia coli/efeitos da radiação
Fusobacterium/efeitos dos fármacos
Fusobacterium/efeitos da radiação
Seres Humanos
Boca/microbiologia
Porphyromonas/efeitos dos fármacos
Porphyromonas/efeitos da radiação
Prevotella/efeitos dos fármacos
Prevotella/efeitos da radiação
Staphylococcus/efeitos dos fármacos
Staphylococcus/efeitos da radiação
Streptococcus/efeitos dos fármacos
Streptococcus/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Infective Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.4155/fmc-2017-0051



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