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Pesquisa : B03.353.750.737.872.405 [Categoria DeCS]
Referências encontradas : 30 [refinar]
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  1 / 30 MEDLINE  
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[PMID]:28658259
[Au] Autor:Vincent WJB; Harvie EA; Sauer JD; Huttenlocher A
[Ad] Endereço:Microbiology Doctoral Training Program, Department of Medical Microbiology and Immunology, University of Wisconsin-Madison; Madison, WI; United States of America.
[Ti] Título:Neutrophil derived LTB4 induces macrophage aggregation in response to encapsulated Streptococcus iniae infection.
[So] Source:PLoS One;12(6):e0179574, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Immune cells sense and react to a multitude of factors including both host and microbe-derived signals. Understanding how cells translate these cues into particular cellular behaviors is a complex yet critical area of study. We have previously shown that both neutrophils and macrophages are important for controlling the fish pathogen Streptococcus iniae. Here, we report both host and bacterial determinants leading to the formation of organized macrophage aggregates as part of the host inflammatory response in a subset of infected larvae. Streptococcal capsule was a required signal for aggregate formation. Macrophage aggregation coincided with NFκB activity, and the formation of these aggregates is mediated by leukotriene B4 (LTB4) produced by neutrophils. Depletion, inhibition, or genetic deletion of leukotriene A4 hydrolase (Lta4h), which catalyzes the last step in LTB4 synthesis, resulted in the absence of macrophage aggregation. Larvae with impaired neutrophil function also had impaired macrophage aggregation; however, aggregate formation was partially rescued with the addition of exogenous LTB4. Neutrophil-specific expression of lta4h was sufficient to rescue macrophage aggregation in Lta4h-deficient larvae and increased host survival following infection. In summary, our findings highlight a novel innate immune response to infection in which specific bacterial products drive neutrophils that modulate macrophage behavior through eicosanoid signaling.
[Mh] Termos MeSH primário: Leucotrieno B4/metabolismo
Macrófagos/metabolismo
Neutrófilos/metabolismo
Infecções Estreptocócicas/metabolismo
[Mh] Termos MeSH secundário: Animais
Epóxido Hidrolases/genética
Epóxido Hidrolases/metabolismo
Deleção de Genes
Imunidade Inata
Inflamação/metabolismo
NF-kappa B/metabolismo
Streptococcus iniae
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B); 1HGW4DR56D (Leukotriene B4); EC 3.3.2.- (Epoxide Hydrolases); EC 3.3.2.- (leukotriene A4 hydrolase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179574


  2 / 30 MEDLINE  
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[PMID]:28425874
[Au] Autor:Na-Phatthalung P; Chusri S; Suanyuk N; Voravuthikunchai SP
[Ad] Endereço:1​Department of Microbiology and Excellence Research Laboratory on Natural Products, Faculty of Science and Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
[Ti] Título:In vitro and in vivo assessments of Rhodomyrtus tomentosa leaf extract as an alternative anti-streptococcal agent in Nile tilapia (Oreochromis niloticus L.).
[So] Source:J Med Microbiol;66(4):430-439, 2017 Apr.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Rhodomyrtustomentosa is a Thai medicinal plant that has been attracting attention for its remarkable antibacterial properties against Gram-positive pathogenic bacteria. The purpose of this study was to evaluate the antibacterial properties of R. tomentosa leaf extract against Streptococcus agalactiae and Streptococcus iniae isolated from infected tilapia. METHODOLOGY: The anti-streptococcal activity of R. tomentosa was determined using broth microdilution assays. RESULTS: The extract demonstrated strong antibacterial activity against the fish pathogens, with minimum inhibitory concentrations (MICs) ranging from 7.8‒62.5 µg ml-1. It was found to possess a dose-dependent bacteriostatic effect on this organism. Scanning electron microscopy revealed irregular and long chains of swollen cells, as well as corkscrew shapes andincomplete separation of cell division of S. agalactiae cells following the treatment at sub-MIC. Moreover, S. agalactiae cells pre-treated with the extract became more sensitive to oxidative stress induced by H2O2 than the untreated cells. Based on the mortality of Nile tilapia after intraperitoneal infection of S. agalactiae at median lethal dose (LD50), the pre-treated cells caused a significant (P<0.01) reduction in mortality of S. agalactiae-infected Nile tilapia. CONCLUSION: The results suggested that R. tomentosa could be further developed as a simple and effective agent for the treatment of streptococcosis in Nile tilapia.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Ciclídeos/microbiologia
Doenças dos Peixes/tratamento farmacológico
Myrtaceae/química
Extratos Vegetais/farmacologia
Infecções Estreptocócicas/tratamento farmacológico
Streptococcus agalactiae/efeitos dos fármacos
Streptococcus iniae/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Sinergismo Farmacológico
Doenças dos Peixes/microbiologia
Peróxido de Hidrogênio/farmacologia
Testes de Sensibilidade Microbiana
Microscopia Eletrônica de Varredura
Estresse Oxidativo/efeitos dos fármacos
Folhas de Planta/química
Infecções Estreptocócicas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Extracts); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000453


  3 / 30 MEDLINE  
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[PMID]:28357982
[Au] Autor:Soto E; Richey C; Stevens B; Yun S; Kenelty K; Reichley S; Griffin M; Kurobe T; Camus A
[Ad] Endereço:Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USA.
[Ti] Título:Co-infection of Acipenserid herpesvirus 2 (AciHV-2) and Streptococcus iniae in cultured white sturgeon Acipenser transmontanus.
[So] Source:Dis Aquat Organ;124(1):11-20, 2017 Mar 30.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A mortality event in cultured white sturgeon Acipenser transmontanus (Richardson, 1836) sub-adults was investigated. After transfer between farms, high mortality was observed in fish, associated with back arching, abnormal swimming, and ulcerative skin lesions. Necropsy of moribund individuals revealed hemorrhagic ascites and petechial hemorrhages in the coelomic peritoneum and serosa of internal organs. Acipenserid herpesvirus 2 (AciHV-2) was isolated from external tissue samples, then identified and genotyped by sequencing of the terminase and polymerase genes. In addition, Streptococcus iniae was recovered from internal organs of affected fish. Histologic changes were limited to interstitial hematopoietic areas of the kidney and consisted of small foci of necrosis accompanied by fibrin deposition, minimal inflammatory response, and small numbers of bacterial cocci compatible with streptococci. Identity was confirmed by partial sequencing of the 16S rRNA, rpoB, and gyrB genes. Genetic fingerprinting demonstrated a genetic profile distinct from S. iniae isolates recovered from previous outbreaks in wild and cultured fish in North America, South America, and the Caribbean. Although the isolates were resistant to white sturgeon complement in serum killing assays, in vivo challenges failed to fulfill Koch's postulates. However, the clinical presentation, coupled with consistent recovery of S. iniae and AciHV-2 from moribund fish, suggests viral and bacterial co-infection were the proximate cause of death. To our knowledge, this represents the first report of AciHV-2 and S. iniae co-infection in cultured white sturgeon.
[Mh] Termos MeSH primário: Coinfecção/veterinária
Doenças dos Peixes/microbiologia
Infecções por Herpesviridae/veterinária
Herpesviridae
Infecções Estreptocócicas/veterinária
Streptococcus iniae
[Mh] Termos MeSH secundário: Animais
Aquicultura
Análise por Conglomerados
Doenças dos Peixes/virologia
Peixes
Genótipo
Herpesviridae/classificação
Infecções por Herpesviridae/complicações
Infecções por Herpesviridae/virologia
Filogenia
Infecções Estreptocócicas/complicações
Infecções Estreptocócicas/microbiologia
Streptococcus iniae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.3354/dao03108


  4 / 30 MEDLINE  
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[PMID]:28235637
[Au] Autor:Qiang J; Tao YF; He J; Li HX; Xu P; Bao JW; Sun YL
[Ad] Endereço:Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China. Electronic address: Qiangj@ffrc.cn.
[Ti] Título:Inhibition of miR-92d-3p enhances inflammation responses in genetically improved farmed tilapia (GIFT, Oreochromis niloticus) with Streptococcus iniae infection by modulating complement C3.
[So] Source:Fish Shellfish Immunol;63:367-375, 2017 Apr.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) are small, non-coding RNAs that regulate target gene expression by binding to the 3'-untranslated regions (3'-UTRs) of their target mRNAs. The miR-92 family is an important miRNA family, which was discovered to be related to regulation of tumor proliferation, apoptosis, invasion, and metastasis. Inhibition of miR-92d-3p was found previously in head kidney of genetically improved farmed tilapia (GIFT, Oreochromis niloticus) exposed to Streptococcus iniae infection. In this study, we found that miR-92d-3p regulated complement C3 mRNA levels by binding to its 3'-UTR by 3'-UTR luciferase reporter assay, and reduced miR-92d-3p expression resulted in increased C3 mRNA levels. We detected a negative relationship between the expression levels of miR-92d-3p and C3 in GIFT injected with miRNA antagomir. We performed in vivo functional analysis by miR-92d-3p silencing. Inhibition of miR-92d-3p levels in GIFT head kidney caused a significant increase in C3 expression, which consequently increased the white blood cell counts and interleukin-1ß, tumor necrosis factor-α, and interferon-γ mRNA levels, all of which may help to activate the inflammatory response in GIFT post-infection with S. iniae. Our findings indicate that miR-92d-3p regulated C3 levels by binding with the C3 mRNA 3'-UTR, and this interaction affected S. iniae infection induction and the immune response in GIFT. We concluded that miR-92d-3p plays an important role in modulating the inflammatory response in GIFT head kidney. Our findings may contribute to understanding the mechanisms of miRNA-mediated gene regulation in tilapia in response to S. iniae infection.
[Mh] Termos MeSH primário: Ciclídeos
Complemento C3/genética
Doenças dos Peixes/genética
Proteínas de Peixes/genética
Inflamação/veterinária
MicroRNAs/genética
Infecções Estreptocócicas/veterinária
[Mh] Termos MeSH secundário: Animais
Ciclídeos/genética
Complemento C3/metabolismo
Doenças dos Peixes/imunologia
Doenças dos Peixes/microbiologia
Proteínas de Peixes/metabolismo
Regulação da Expressão Gênica
Imunidade Inata
Inflamação/genética
Inflamação/imunologia
Inflamação/microbiologia
MicroRNAs/metabolismo
Infecções Estreptocócicas/genética
Infecções Estreptocócicas/imunologia
Infecções Estreptocócicas/microbiologia
Streptococcus iniae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complement C3); 0 (Fish Proteins); 0 (MicroRNAs)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


  5 / 30 MEDLINE  
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[PMID]:28124778
[Au] Autor:Kim SW; Nho SW; Im SP; Lee JS; Jung JW; Lazarte JM; Kim J; Lee WJ; Lee JH; Jung TS
[Ad] Endereço:Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea.
[Ti] Título:Rapid MALDI biotyper-based identification and cluster analysis of Streptococcus iniae.
[So] Source:J Microbiol;55(4):260-266, 2017 Apr.
[Is] ISSN:1976-3794
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Streptococcus iniae causes severe mortalities among cultured marine species, especially in the olive flounder (Paralichthys olivaceus), which is economically important in Korea and Japan. Recently, there has been growing concern regarding the emergence of S. iniae as a zoonotic pathogen. Here, 89 S. iniae isolates obtained from diseased olive flounders collected from 2003 to 2008 in Jeju Island, South Korea, were characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The results were aligned both with the available Bruker Daltonics data-base and with a new set of S. iniae data entries developed in our laboratory, and the results were compared. When we used the Bruker Daltonics database, the 89 isolates yielded either "no reliable identification" or were incorrectly identified as Streptococcus pyogenes at the genus level. When we used the new data entries from our laboratory, in contrast, all of the isolates were correctly identified as S. iniae at the genus (100%) and species (96.6%) levels. We performed proteomic analysis, divided the 89 isolates into cluster I (51.7%), cluster II (20.2%), and cluster III (28.1%), and then used the MALDI Biotyper software to identify specific mass peaks that enabled discrimination between clusters and between Streptococcus species. Our results suggest that the use of MALDI TOF MS could outperform the conventional methods, proving easier, faster, cheaper and more efficient in properly identifying S. iniae. This strategy could facilitate the epidemiological and taxonomical study of this important fish pathogen.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos
Doenças dos Peixes/microbiologia
Linguado/microbiologia
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
Infecções Estreptocócicas/veterinária
Streptococcus iniae/química
Streptococcus iniae/classificação
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
Custos e Análise de Custo
Japão
Coreia (Geográfico)
República da Coreia
Sensibilidade e Especificidade
Infecções Estreptocócicas/microbiologia
Fatores de Tempo
Medicina Veterinária/métodos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1007/s12275-017-6472-x


  6 / 30 MEDLINE  
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[PMID]:28117298
[Au] Autor:Song Z; Yue R; Sun Y; Liu C; Khan SH; Li C; Zhao Y; Zhou X; Yang L; Zhao D
[Ad] Endereço:The State Key Laboratories for Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture; National Animal Transmissible Spongiform Encephalopathy Laboratory; Department of Veterinary Pathology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
[Ti] Título:Fatal bacterial septicemia in a bottlenose dolphin Tursiops truncatus caused by Streptococcus iniae.
[So] Source:Dis Aquat Organ;122(3):195-203, 2017 01 24.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A captive 8 yr old male bottlenose dolphin Tursiops truncatus succumbed to septicemia with multisystemic inflammation including suppurative enteritis, encephalitis, and pneumonia with chronic pancreatitis. A pure culture of beta-hemolytic, catalase- and oxidase-negative, Gram-positive cocci was isolated from the hilar lymph nodes and pancreas. The isolate was identified by 16S rDNA sequencing as Streptococcus iniae. Histological examination of the digestive system revealed a mixed infection of both bacteria and fungus. Recognized as a pathogen in fish, dolphins, and humans, this is the first report of S. iniae in a dolphin in mainland China. As the number of managed animals in oceanariums is increasing, so is the frequency of contact with fish used as food for marine mammals and humans, highlighting the importance of education and appropriate personal protective protocols to minimize the risk of transmission. An understanding of marine mammal infectious disease organisms is essential to ensuring the health of marine mammals and humans coming into contact with such animals and their food. This study illustrates a systematic clinical, microbiological, and pathological investigation into a septicemic bottlenose dolphin infected with S. iniae. Our findings provide useful information for those involved in the diagnosis and control of infectious diseases in marine mammals and offer insight into an important zoonotic pathogen.
[Mh] Termos MeSH primário: Golfinho Nariz-de-Garrafa
Sepse/veterinária
Infecções Estreptocócicas/veterinária
Streptococcus iniae/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Evolução Fatal
Masculino
Sepse/microbiologia
Infecções Estreptocócicas/microbiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE
[do] DOI:10.3354/dao03069


  7 / 30 MEDLINE  
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[PMID]:28091882
[Au] Autor:El Aamri F; Guillén JÁ; Padilla D; Acosta F; Real F
[Ad] Endereço:Institut National de Recherche Halieutique (INRH), M'diq, Morocco.
[Ti] Título:Identification of some main Streptococcus iniae associated proteins: relationship.
[So] Source:Vet Res Commun;41(2):85-95, 2017 Jun.
[Is] ISSN:1573-7446
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The surface-associated proteins play a key role in bacterial physiology and pathogenesis, and are the major targets in the development of new vaccines. These proteins contribute to the adaptation of bacteria to different hosts and environments. To study differences at the genomic level, we first sequenced the whole genome of Streptococcus iniae from fish (IUSA-1 strain) and compared it to Streptococcus iniae from human (9117 strain), revealing a high similitude between both strains. To gain further insights into host- and environment-specific differences, we then studied proteins in silico and by High Performance Liquid Chromatography. This approach successfully identified 54 secreted and surface proteins, including several proteins involved in cell wall synthesis and transport of solutes, as well as proteins with yet unknown function. These proteins highlight as interesting targets for further investigation in the interaction between Streptococcus iniae and its environment. Results reported in this study have shown a first analysis about the predicted and experimental associated proteins of Streptococcus iniae isolated from two different hosts: human and fish.
[Mh] Termos MeSH primário: Streptococcus iniae/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/fisiologia
Cromatografia Líquida de Alta Pressão/veterinária
Simulação por Computador
Doenças dos Peixes/microbiologia
Peixes/microbiologia
Genoma Bacteriano/genética
Proteínas de Membrana/genética
Proteínas de Membrana/fisiologia
Infecções Estreptocócicas/microbiologia
Infecções Estreptocócicas/veterinária
Streptococcus iniae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Membrane Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE
[do] DOI:10.1007/s11259-017-9675-z


  8 / 30 MEDLINE  
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[PMID]:27226029
[Au] Autor:Lim JW; Jung MH; Jung SJ; Kim DH; Park KH; Kang SY
[Ad] Endereço:Department of Aqualife Medicine, Chonnam National University, Yeosu, Korea.
[Ti] Título:The efficacy of amoxicillin sodium against streptococcosis in cultured olive flounder Paralichthys olivaceus and its pharmacokinetics.
[So] Source:J Vet Pharmacol Ther;40(1):77-87, 2017 Jan.
[Is] ISSN:1365-2885
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The efficacy of amoxicillin sodium for controlling field and experimental Streptococcus iniae and S. parauberis infections in olive flounder (Paralichthys olivaceus) was evaluated after a single intramuscular administration. Furthermore, the minimal inhibitory concentrations (MIC) against 21 Streptococcus strains were determined. In addition, the pharmacokinetics and residue depletion in olive flounder were investigated. Single intramuscular doses of amoxicillin sodium at 20, 40, 80, and 160 mg/kg b.w. fish significantly reduced cumulative mortality rates to 18.8-31.3% (P < 0.05) for S. iniae and to 5.0-15.0% (P < 0.01) for S. parauberis, whereas the S. iniae- and S. parauberis-infected positive control groups showed cumulative mortality rates of 68.8% and 60.0%, respectively. In a S. parauberis outbreak, amoxicillin sodium reduced the cumulative mortality rate to 7.5% and 4.8% at 20 and 40 mg/kg b.w. fish, respectively, whereas that of the untreated control group was 35.2%. Peak plasma concentrations (C ) following a single intramuscular dose of 40 and 80 mg/kg b.w. fish were 62.64 (T , 1.59 h) and 87.61 (T , 3.02 h) µg/mL, respectively, with large AUC /MIC and C /MIC ratios, and sufficient T > MIC (time for maintaining plasma drug concentration greater than MICs) for S. iniae and S. parauberis. The estimated withdrawal period of amoxicillin sodium from muscle of olive flounder was about 8 days at 40 mg/kg b.w. fish (at 22 ± 1 °C). These results demonstrated a single intramuscular administration of amoxicillin sodium to be effective against streptococcosis in olive flounder.
[Mh] Termos MeSH primário: Amoxicilina/uso terapêutico
Antibacterianos/uso terapêutico
Doenças dos Peixes/tratamento farmacológico
Linguado/microbiologia
Infecções Estreptocócicas/veterinária
[Mh] Termos MeSH secundário: Amoxicilina/administração & dosagem
Amoxicilina/farmacocinética
Animais
Antibacterianos/administração & dosagem
Antibacterianos/farmacocinética
Aquicultura/métodos
Injeções Intramusculares/veterinária
Infecções Estreptocócicas/tratamento farmacológico
Streptococcus/efeitos dos fármacos
Streptococcus iniae/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 804826J2HU (Amoxicillin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160527
[St] Status:MEDLINE
[do] DOI:10.1111/jvp.12321


  9 / 30 MEDLINE  
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[PMID]:27825948
[Au] Autor:Dong X; Fu Q; Liu S; Gao C; Su B; Tan F; Li C
[Ad] Endereço:Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
[Ti] Título:The expression signatures of neuronal nitric oxide synthase (NOS1) in turbot (Scophthalmus maximus L.) mucosal surfaces against bacterial challenge.
[So] Source:Fish Shellfish Immunol;59:406-413, 2016 Dec.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mucosal surfaces constitute the first immune barrier of host defense and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes. It has been long hypothesized that observed difference of disease resistance among different fish strains and species are strongly correlated to the activities of the immune actors in mucosal surfaces. Particularly, neuronal NOS (nNOS or NOS1) is a constitutively expressed gene that catalyzes the oxidation of l-arginine and water to nitric oxide (NO), which is known as a potent host defence effector in immune system with antimicrobial activity. Moreover, NOS1 was detected to be expressed in fish mucosal surfaces, but its activities in mucosal immune responses were always overlooked. In this regard, we identified the NOS1 of turbot and characterized its expression patterns in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge. The results showed that the NOS1 gene had a 4389 bp open reading frame (ORF) that encoded 1462 amino acids. Phylogenetic analysis showed the turbot NOS1 had the strongest relationship to Larimichthys crocea. And the syntenic analysis revealed the similar neighboring genes associated with turbot NOS1, compared with other teleost and mammals. In addition, NOS1 was widely expressed in all examined tissues with the highest expression level in brain, followed by intestine and gill. Finally, the NOS1 showed a general trend of up-regulation in mucosal tissues following both bacterial challenge, with the highest up-regulation in intestine. The significant quick induction of NOS1 in mucosal surfaces against infection indicated its key roles to prevent pathogen attachment and entry in mucosal immunity. More functional studies are needed to conduct in teleost to better understand the roles of NOS1 in maintaining the integrity of the mucosal barriers against infection.
[Mh] Termos MeSH primário: Doenças dos Peixes/genética
Proteínas de Peixes/genética
Linguados
Regulação da Expressão Gênica
Óxido Nítrico Sintase Tipo I/genética
Infecções Estreptocócicas/veterinária
Vibrioses/veterinária
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/imunologia
Doenças dos Peixes/microbiologia
Proteínas de Peixes/química
Proteínas de Peixes/metabolismo
Imunidade Inata
Membrana Mucosa/imunologia
Óxido Nítrico Sintase Tipo I/química
Óxido Nítrico Sintase Tipo I/metabolismo
Especificidade de Órgãos
Filogenia
Distribuição Aleatória
Alinhamento de Sequência/veterinária
Análise de Sequência de DNA/veterinária
Infecções Estreptocócicas/genética
Infecções Estreptocócicas/imunologia
Infecções Estreptocócicas/microbiologia
Streptococcus iniae/fisiologia
Vibrio/fisiologia
Vibrioses/genética
Vibrioses/imunologia
Vibrioses/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); EC 1.14.13.39 (Nitric Oxide Synthase Type I)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE


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[PMID]:27732899
[Au] Autor:Ko J; Wan Q; Bathige SDNK; Lee J
[Ad] Endereço:Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Jeju International Marine Science Research & Education Center, Korea Institute of Ocean Science & Technology (KIOST), Jeju Special Self-Gov
[Ti] Título:Molecular characterization, transcriptional profiling, and antibacterial potential of G-type lysozyme from seahorse (Hippocampus abdominalis).
[So] Source:Fish Shellfish Immunol;58:622-630, 2016 Nov.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lysozymes are a family of enzymes that catalyze the hydrolysis of bacterial cell wall, acting as antimicrobial effectors of the innate immune system. In the present study, an ortholog of goose-type lysozyme (ShLysG) from the big-belly seahorse (Hippocampus abdominalis) was identified and characterized structurally and functionally. The full-length cDNA sequence (1213 bp) of ShLysG is comprised of an open reading frame made up of 552 bp, encoding a polypeptide of 184 amino acid (aa) with a predicted molecular mass of 20 kDa. In silico analysis of ShLysG revealed the absence of signal peptide and the presence of a characteristic bacterial soluble lytic transglycosylase (SLT) domain bearing three catalytic residues (Glu , Asp , and Asp ) and seven N-acetyl-d-glucosamine binding sites (Glu , Asp , Tyr , His , Ile , Tyr , and Asn ). Homology analysis demonstrated that the aa sequence of ShLysG shared 60.7-67.4% identity and 72.6-79.3% similarity with the orthologs of other teleosts. Phylogenetic analysis of ShLysG indicated a closest relationship with the ortholog from Gadus morhua. In healthy seahorse, ShLysG mRNA showed a constitutive expression in all the tissues examined, with the highest expression in kidney and the least expression in liver. The ShLysG mRNA levels were also shown significant elevation upon the bacterial and pathogen-associated molecular pattern (PAMPs) challenges. Furthermore, lytic activities of ShLysG recombinant protein were detected against several Gram-negative and Gram-positive bacterial species. Taken together, these results suggest that ShLysG might possess a potential immune defensive role against invading microbial pathogens in seahorse.
[Mh] Termos MeSH primário: Infecções por Enterobacteriaceae/veterinária
Doenças dos Peixes/genética
Proteínas de Peixes/genética
Muramidase/genética
Padrões Moleculares Associados a Patógenos/farmacologia
Smegmamorpha
Infecções Estreptocócicas/veterinária
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Clonagem Molecular
DNA Complementar/genética
DNA Complementar/metabolismo
Edwardsiella tarda/fisiologia
Infecções por Enterobacteriaceae/genética
Infecções por Enterobacteriaceae/imunologia
Infecções por Enterobacteriaceae/microbiologia
Doenças dos Peixes/microbiologia
Proteínas de Peixes/química
Proteínas de Peixes/metabolismo
Lipopolissacarídeos/farmacologia
Muramidase/química
Muramidase/metabolismo
Filogenia
Poli I-C/farmacologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência/veterinária
Infecções Estreptocócicas/genética
Infecções Estreptocócicas/microbiologia
Streptococcus iniae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Fish Proteins); 0 (Lipopolysaccharides); 0 (Pathogen-Associated Molecular Pattern Molecules); 0 (RNA, Messenger); 0 (Recombinant Proteins); EC 3.2.1.17 (Muramidase); O84C90HH2L (Poly I-C)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161013
[St] Status:MEDLINE



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