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[PMID]: 29194752 [Au] Autor: Engen SA; Schreurs O; Petersen F; Blix IJS; Baekkevold ES; Schenck K [Ad] Endereço: Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway. [Ti] Título: The Regulatory Role of the Oral Commensal Streptococcus mitis on Human Monocytes. [So] Source: Scand J Immunol;87(2):80-87, 2018 Feb. [Is] ISSN: 1365-3083 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Streptococcus mitis colonizes all niches of the human oral cavity from early infancy and throughout life. Monocytes patrol blood vessels, lymphoid and non-lymphoid tissues and migrate into infected tissue where they participate in the inflammatory cascade and immune regulation. Here, we studied the effect of S. mitis on monocytes. Transcriptome analysis of monocytes exposed to S. mitis (SmMo) revealed increased transcription of chemotactic factors (CCL2, CCL3, CCL20, CXCL1, CXCL2) and cytokines (IL1A, IL1B, IL6, IL23, IL36G, TNF), indicating that S. mitis may trigger recruitment of leucocytes and initiate inflammation. Increased transcription in SmMo of IL1B, IL6 and IL23 indicated that S. mitis may participate in the induction of Th17 responses and agreed with our earlier findings of S. mitis-mediated memory Th17 reactivity. Furthermore, S. mitis inhibited tetanus toxoid-specific CD4 T cell proliferation. This can be due to the increased secretion of IL-10 and expression of PD-L1 that was observed in SmMo. PGE2 can modulate IL-10 and PD-L1 expression, concomitant with that of CCR7, IL-12 and IL-23 that also were changed. This, along with increased SmMo transcription of PTGS2 (COX2) and PTGER4 (EP4), pointed to a role of PGE2. Measurement of PGE2 secretion by SmMo showed indeed a marked increase, and chemical inhibition of PGE2 production lowered the PD-L1 expression on SmMo. In conclusion, our findings show that S. mitis may trigger immune modulation by recruiting immune cells to the site of infection, while at the same time dampening the severity of the response through expression of IL-10, PGE2 and PD-L1. [Mh] Termos MeSH primário: Monócitos/imunologia Boca/microbiologia Infecções Estreptocócicas/imunologia Streptococcus mitis/imunologia [Mh] Termos MeSH secundário: Antígeno B7-H1/metabolismo Células Cultivadas Quimiotaxia Ciclo-Oxigenase 2/genética Ciclo-Oxigenase 2/metabolismo Citocinas/genética Citocinas/metabolismo Dinoprostona/metabolismo Perfilação da Expressão Gênica Regulação da Expressão Gênica Seres Humanos Imunomodulação Mediadores da Inflamação/metabolismo Monócitos/microbiologia Receptores de Prostaglandina E Subtipo EP4/genética Receptores de Prostaglandina E Subtipo EP4/metabolismo Simbiose [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (B7-H1 Antigen); 0 (Cytokines); 0 (Inflammation Mediators); 0 (PTGER4 protein, human); 0 (Receptors, Prostaglandin E, EP4 Subtype); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (PTGS2 protein, human); K7Q1JQR04M (Dinoprostone) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180206 [Lr] Data última revisão: 180206 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171202 [St] Status: MEDLINE [do] DOI: 10.1111/sji.12636

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[PMID]: 28513788 [Au] Autor: Bertoldo BB; Silva CBD; Rodrigues DBR; Geraldo-Martins VR; Ferriani VPL; Nogueira RD [Ad] Endereço: Universidade de Uberaba - Uniube, School of Dentistry, Department of Dentistry, Uberaba, MG, Brazil. [Ti] Título: Comparisons of IgA response in saliva and colostrum against oral streptococci species. [So] Source: Braz Oral Res;31:e39, 2017 May 15. [Is] ISSN: 1807-3107 [Cp] País de publicação: Brazil [La] Idioma: eng [Ab] Resumo: The present study compared IgA specificity against oral streptococci in colostrum and saliva samples. Sixty-two mother-and-child pairs were included; samples of colostrum (C) and saliva (MS) were collected from the mothers and saliva samples were collected from babies (BS). The specificity of IgA against Streptococcus mutans and S. mitis were analyzed by western blot. Only 30% of babies' samples presented IgA reactivity to S. mutans, while 74 and 80% of MS and C, respectively, presented this response. IgA reactivity to S. mutans virulence antigens (Ag I/II, Gtf and GbpB) in positive samples showed differences between samples for Gtf and especially for GbpB (p < 0.05), but responses to Ag I/II were similar (p > 0.05). The positive response of Gtf-reactive IgA was different between C (90%) and MS (58%) samples (p < 0.05), but did not differ from BS (p > 0.05). GbpB was the least detected, with 48 and 26% of C and MS, and only 5% of BS samples presenting reactivity (p > 0.05). Eight percent of MS and C samples presented identical bands to SM in the same time-point. In conclusion, the differences of IgA response found between C and MS can be due to the different ways of stimulation, proliferation and transportation of IgA in those secretions. The colostrum has high levels of IgA against S. mutans virulence antigens, which could affect the installation and accumulation process of S. mutans, mainly by supplying anti-GbpB IgA to the neonate. [Mh] Termos MeSH primário: Colostro/imunologia Imunoglobulina A Secretora/análise Imunoglobulina A Secretora/imunologia Saliva/imunologia Streptococcus mitis/imunologia Streptococcus mutans/imunologia [Mh] Termos MeSH secundário: Análise de Variância Formação de Anticorpos/imunologia Antígenos de Bactérias/análise Antígenos de Bactérias/imunologia Proteínas de Bactérias/análise Proteínas de Bactérias/imunologia Western Blotting Colostro/microbiologia Ensaio de Imunoadsorção Enzimática Feminino Glucosiltransferases/análise Glucosiltransferases/imunologia Glicoproteínas/análise Glicoproteínas/imunologia Seres Humanos Recém-Nascido Mães Saliva/microbiologia Virulência [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antigens, Bacterial); 0 (Bacterial Proteins); 0 (Glycoproteins); 0 (IDG-60 protein, Streptococcus mutans); 0 (Immunoglobulin A, Secretory); EC 2.4.1.- (Glucosyltransferases) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170605 [Lr] Data última revisão: 170605 [Sb] Subgrupo de revista: D; IM [Da] Data de entrada para processamento: 170518 [St] Status: MEDLINE

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[PMID]: 28272747 [Au] Autor: Patil S; Chaudhari B [Ad] Endereço: Department of Microbiology and Biotechnology, R.C. Patel Arts, Commerce and Science College, Shirpur, India. [Ti] Título: A simple, rapid and sensitive plate assay for detection of microbial hyaluronidase activity. [So] Source: J Basic Microbiol;57(4):358-361, 2017 Apr. [Is] ISSN: 1521-4028 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Hyaluronidase (hyase) is a glycosidase enzyme that predominantly degrades hyaluronic acid (HA) having important applications in many biotechnological processes and therapeutics. Several assay methods have been proposed to screen hyase producing microorganisms; however, they rely on unique reagents and sophisticated instruments, which are expensive and could be unavailable in general laboratories. In the present studies, a rapid, simple, sensitive, highly reproducible, and cost-effective qualitative plate assay has been developed for the screening of hyase producing microorganisms. The routinely used plate assay method of Richman and Baer requires a special chemical cetylpyridinium chloride and long incubation period of 20 h; but still, the zones of clearance are not very clear and distinct. While, the present method requires an incubation period of only 1 h and the distinct zones of clearance appear with Gram's iodine within 1 min of time. This method does not require any special medium, unlike previously reported methods. Moreover, use of commonly available Gram's iodine makes this method suitable for many researchers. The results of the assay method were validated by TLC, zymographic analysis and determining the growth of isolates in minimal medium containing HA as a sole carbon source. [Mh] Termos MeSH primário: Ensaios Enzimáticos/métodos Hialuronoglucosaminidase/isolamento & purificação Streptococcus equi/enzimologia [Mh] Termos MeSH secundário: Meios de Cultura/química Ensaios Enzimáticos/economia Seres Humanos Ácido Hialurônico/isolamento & purificação Ácido Hialurônico/metabolismo Hialuronoglucosaminidase/metabolismo Iodo Sensibilidade e Especificidade Sefarose Streptococcus equi/química Streptococcus equi/crescimento & desenvolvimento Streptococcus mitis/enzimologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Culture Media); 9004-61-9 (Hyaluronic Acid); 9012-36-6 (Sepharose); 9679TC07X4 (Iodine); EC 3.2.1.35 (Hyaluronoglucosaminidase) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170421 [Lr] Data última revisão: 170421 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170309 [St] Status: MEDLINE [do] DOI: 10.1002/jobm.201600579

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[PMID]: 28053215 [Au] Autor: Harju I; Lange C; Kostrzewa M; Maier T; Rantakokko-Jalava K; Haanperä M [Ad] Endereço: Clinical Microbiology Laboratory, Turku University Hospital, Turku, Finland inka.harju@tyks.fi. [Ti] Título: Improved Differentiation of Streptococcus pneumoniae and Other S. mitis Group Streptococci by MALDI Biotyper Using an Improved MALDI Biotyper Database Content and a Novel Result Interpretation Algorithm. [So] Source: J Clin Microbiol;55(3):914-922, 2017 Mar. [Is] ISSN: 1098-660X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Reliable distinction of and viridans group streptococci is important because of the different pathogenic properties of these organisms. Differentiation between and closely related species group streptococci has always been challenging, even when using such modern methods as 16S rRNA gene sequencing or matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. In this study, a novel algorithm combined with an enhanced database was evaluated for differentiation between and species group streptococci. One hundred one clinical species group streptococcal strains and 188 clinical strains were identified by both the standard MALDI Biotyper database alone and that combined with a novel algorithm. The database update from 4,613 strains to 5,627 strains drastically improved the differentiation of and species group streptococci: when the new database version containing 5,627 strains was used, only one of the 101 species group isolates was misidentified as , whereas 66 of them were misidentified as when the earlier 4,613-strain MALDI Biotyper database version was used. The updated MALDI Biotyper database combined with the novel algorithm showed even better performance, producing no misidentifications of the species group strains as All strains were correctly identified as with both the standard MALDI Biotyper database and the standard MALDI Biotyper database combined with the novel algorithm. This new algorithm thus enables reliable differentiation between pneumococci and other species group streptococci with the MALDI Biotyper. [Mh] Termos MeSH primário: Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos Streptococcus mitis/classificação Streptococcus pneumoniae/classificação [Mh] Termos MeSH secundário: Algoritmos Bases de Dados de Compostos Químicos Seres Humanos Infecções Estreptocócicas/microbiologia Streptococcus mitis/química Streptococcus mitis/isolamento & purificação Streptococcus pneumoniae/química Streptococcus pneumoniae/isolamento & purificação [Pt] Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170902 [Lr] Data última revisão: 170902 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170106 [St] Status: MEDLINE [do] DOI: 10.1128/JCM.01990-16

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[PMID]: 27787811 [Au] Autor: Gallorini M; di Giacomo V; Di Valerio V; Rapino M; Bosco D; Travan A; Di Giulio M; Di Pietro R; Paoletti S; Cataldi A; Sancilio S [Ad] Endereço: Department of Pharmacy, "G. d'Annunzio" University, Chieti-Pescara, Italy. [Ti] Título: Cell-protection mechanism through autophagy in HGFs/S. mitis co-culture treated with Chitlac-nAg. [So] Source: J Mater Sci Mater Med;27(12):186, 2016 Dec. [Is] ISSN: 1573-4838 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Silver-based products have been proven to be effective in retarding and preventing bacterial growth since ancient times. In the field of restorative dentistry, the use of silver ions/nanoparticles has been explored to counteract bacterial infections, as silver can destroy bacterial cell walls by reacting with membrane proteins. However, it is also cytotoxic towards eukaryotic cells, which are capable of internalizing nanoparticles. In this work, we investigated the biological effects of Chitlac-nAg, a colloidal system based on a modified chitosan (Chitlac), administered for 24-48 h to a co-culture of primary human gingival fibroblasts and Streptococcus mitis in the presence of saliva, developed to mimic the microenvironment of the oral cavity. We sought to determine its efficiency to combat oral hygiene-related diseases without affecting eukaryotic cells. Cytotoxicity, reactive oxygen species production, apoptosis induction, nanoparticles uptake, and lysosome and autophagosome metabolism were evaluated. In vitro results show that Chitlac-nAg does not exert cytotoxic effects on human gingival fibroblasts, which seem to survive through a homoeostasis mechanism involving autophagy. That suggests that the novel biomaterial Chitlac-nAg could be a promising tool in the field of dentistry. [Mh] Termos MeSH primário: Autofagia Técnicas de Cocultura Fibroblastos/microbiologia [Mh] Termos MeSH secundário: Aderência Bacteriana/efeitos dos fármacos Sobrevivência Celular Quitosana/farmacologia Coloides/química Materiais Dentários Fibroblastos/citologia Citometria de Fluxo Gengiva/citologia Seres Humanos Íons L-Lactato Desidrogenase/química Nanopartículas Metálicas/química Microscopia Eletrônica de Transmissão Espécies Reativas de Oxigênio/química Prata/farmacologia Streptococcus mitis/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Colloids); 0 (Dental Materials); 0 (Ions); 0 (Reactive Oxygen Species); 3M4G523W1G (Silver); 9012-76-4 (Chitosan); EC 1.1.1.27 (L-Lactate Dehydrogenase) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 171021 [Lr] Data última revisão: 171021 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161028 [St] Status: MEDLINE

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[PMID]: 27737290 [Au] Autor: Feder OI; Gruson KI [Ti] Título: Glenohumeral Joint Sepsis Caused by Streptococcus mitis: A Case Report. [So] Source: Am J Orthop (Belle Mead NJ);45(6):E343-E346, 2016 Sep/Oct. [Is] ISSN: 1934-3418 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Septic arthritis of the shoulder girdle remains relatively uncommon, with Staphylococcus aureus and ß-hemolytic streptococci the typical offending organisms in adult patients. Rare cases of Streptococcus viridans, an oral bacterium with low virulence, have been reported in the setting of septic arthritis, mostly involving the knee joint or the sternoclavicular joint. In this article, we report a case of Streptococcus mitis infection of the glenohumeral joint that likely resulted from hematogenous spread after oral trauma in a patient with poor underlying dentition. Prompt diagnosis followed by arthroscopic irrigation and débridement of the glenohumeral joint resulted in a satisfactory clinical outcome. [Mh] Termos MeSH primário: Artrite Infecciosa/cirurgia Artroplastia do Ombro Articulação do Ombro/cirurgia Infecções Estreptocócicas/cirurgia Streptococcus mitis/isolamento & purificação [Mh] Termos MeSH secundário: Antibacterianos/uso terapêutico Artrite Infecciosa/diagnóstico por imagem Artrite Infecciosa/tratamento farmacológico Artrite Infecciosa/microbiologia Desbridamento Seres Humanos Masculino Meia-Idade Articulação do Ombro/microbiologia Infecções Estreptocócicas/diagnóstico por imagem Infecções Estreptocócicas/tratamento farmacológico Infecções Estreptocócicas/microbiologia Irrigação Terapêutica Resultado do Tratamento [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170206 [Lr] Data última revisão: 170206 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161014 [St] Status: MEDLINE

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[PMID]: 27659083 [Au] Autor: Pandey V; Choudhary I; Kumar V; Tripathi P; Misra A; Bagde H [Ad] Endereço: Reader, Department of Conservative Dentistry and Endodontics Institute of Dental Studies and Technology, Modinagar Uttar Pradesh, India, Phone: +919648886633, e-mail: vinisha9@gmail.com. [Ti] Título: Assessment of Correlation between Clinical Parameters and Pulp Canal Pathogens in Endodontic Pathologies: A Microbiological Study. [So] Source: J Contemp Dent Pract;17(8):654-8, 2016 Aug 01. [Is] ISSN: 1526-3711 [Cp] País de publicação: India [La] Idioma: eng [Ab] Resumo: INTRODUCTION: Success and failure of root canal therapy is largely dependent on the clinician and the patient. Once the tooth is restored into its functional and esthetic place, the root canal therapy is considered to be complete. It is a well-known fact that root canal space acts as a rich reservoir for microorganisms. Past studies show that root canal harbors an array of different microorganisms. Streptococcus mitis and Enterococcus faecalis have been found to be the most prevalent microorganisms isolated from the infected pulp canal space. Hence, we evaluated the association of endodontic signs and symptoms with root canal pathogens. MATERIALS AND METHODS: A total of 120 patients were selected for the study and divided broadly into two groups: Group I containing patients with primary infection and group II containing patients in which retreatment was required. Both groups contained 60 patients each. Clinical parameters recorded for the study are pain, tenderness on percussion, swelling, periapical radiolucency on radiographic analysis, caries, sinus formation, and tooth mobility. After assessment, opening, and initial filing, sterile paper points were inserted into the root canal space for collection of microbiological sample, which was further send to laboratory for cultural analysis. All the results were analyzed by Statistical Package for the Social Sciences (SPSS) software. Student's t-test and Fisher's exact test were used to evaluate the level of significance. RESULTS: While comparing the mean age of the patients in the two groups, no significant association was seen. Statistically significant results were obtained while comparing the association between pain and S. mitis. Positive correlation was seen on comparing the association between tenderness on percussion and E. faecalis. Also, a positive association was seen between periapical radiolucency and S. mitis. CONCLUSION: Strong positive correlation exists between S. mitis and E. faecalis and endodontic signs and symptoms. CLINICAL SIGNIFICANCE: Sound knowledge regarding the association of microbes of root canal and specific endodontic signs and symptoms could help therapeutic management of root canal infections effectively. [Mh] Termos MeSH primário: Cavidade Pulpar/microbiologia Dor/etiologia Doenças Periapicais/diagnóstico por imagem Periodontite Periapical/microbiologia Tratamento do Canal Radicular [Mh] Termos MeSH secundário: Adulto Enterococcus faecalis/isolamento & purificação Seres Humanos Meia-Idade Streptococcus mitis/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171107 [Lr] Data última revisão: 171107 [Sb] Subgrupo de revista: D; IM [Da] Data de entrada para processamento: 160924 [St] Status: MEDLINE

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[PMID]: 27471999 [Au] Autor: Leuenhagen S; Burghaus L; Kukolja J; Rosenkranz S; Kabbasch C; Fink GR; Onur OA [Ad] Endereço: Uniklinik Köln, Klinik und Poliklinik für Neurologie, Köln. [Ti] Título: [The Therapeutic Dilemma in Treatment of Intracranial Infectious Aneurysm in Patients with Infective Endocarditis: Proposal for a Patient-Centered, Interdisciplinary Treatment Concept]. [Ti] Título: Das infektiöse Pseudoaneurysma - eine seltene Komplikation bei Patienten mit septischem Krankheitsbild: aktuelle Datenlage und Vorschlag eines diagnostischen und therapeutischen Algorithmus.. [So] Source: Fortschr Neurol Psychiatr;84(7):411-8, 2016 Jul. [Is] ISSN: 1439-3522 [Cp] País de publicação: Germany [La] Idioma: ger [Ab] Resumo: Intracranial infectious aneurysms are rare but hazardous complications of an infective endocarditis. To date, there are no evidence-based recommendations for the treatment of patients with this condition. Therefore, it remains an interdisciplinary challenge to decide which treatment steps are required and in which order they should be carried out. To illustrate the interdisciplinary dilemma in the treatment of these patients, we here present a case of a 23-year-old, drug-addicted woman with infectious endocarditis. While antibiotic treatment of the streptococcus-mitis-induced endocarditis stabilized the overall status of the patient, rupture of a basilar artery aneurysm caused her sudden death. We discuss the decision-making processes of the treatment, potential difficulties and dilemmas when dealing with patients suffering from infectious endocarditis and infectious intracranial aneurysm. Based upon case reports, studies and reviews, we present the options and risks of conservative, neurosurgical, endovascular, and cardiosurgical treatment of intracranial infectious aneurysms, and propose a patient-centered, interdisciplinary treatment concept. [Mh] Termos MeSH primário: Aneurisma Infectado/complicações Aneurisma Infectado/terapia Endocardite Bacteriana/complicações Endocardite Bacteriana/terapia Comunicação Interdisciplinar Colaboração Intersetorial Aneurisma Intracraniano/complicações Aneurisma Intracraniano/terapia Assistência Centrada no Paciente Infecções Estreptocócicas/complicações Infecções Estreptocócicas/terapia Streptococcus mitis [Mh] Termos MeSH secundário: Adulto Algoritmos Aneurisma Roto/complicações Antibacterianos/uso terapêutico Encéfalo/patologia Terapia Combinada Imagem de Difusão por Ressonância Magnética Evolução Fatal Feminino Seres Humanos Exame Neurológico [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Anti-Bacterial Agents) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160730 [St] Status: MEDLINE [do] DOI: 10.1055/s-0042-110850

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[PMID]: 27343694 [Au] Autor: Abdulbaqi HR; Himratul-Aznita WH; Baharuddin NA [Ad] Endereço: Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia. [Ti] Título: Anti-plaque effect of a synergistic combination of green tea and Salvadora persica L. against primary colonizers of dental plaque. [So] Source: Arch Oral Biol;70:117-124, 2016 Oct. [Is] ISSN: 1879-1506 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: OBJECTIVE: Green tea (Gt), leafs of Camellia sinensis var. assamica, is widely consumed as healthy beverage since thousands of years in Asian countries. Chewing sticks (miswak) of Salvadora persica L. (Sp) are traditionally used as natural brush to ensure oral health in developing countries. Both Gt and Sp extracts were reported to have anti-bacterial activity against many dental plaque bacteria. However, their combination has never been tested to have anti-bacterial and anti-adherence effect against primary dental plaque colonizers, playing an initial role in the dental plaque development, which was investigated in this study. METHODS: Two-fold serial micro-dilution method was used to measure minimal inhibitory concentration (MIC) of aqueous extracts of Gt, Sp and their combinations. Adsorption to hexadecane was used to determine the cell surface hydrophobicity (CSH) of bacterial cells. Glass beads were used to mimic the hard tissue surfaces, and were coated with saliva to develop experimental pellicles for the adhesion of the primary colonizing bacteria. RESULTS: Gt aqueous extracts exhibited better anti-plaque effect than Sp aqueous extracts. Their combination, equivalent to 1/4 and 1/2 of MIC values of Gt and Sp extracts respectively, showed synergistic anti-plaque properties with fractional inhibitory concentration (FIC) equal to 0.75. This combination was found to significantly reduce CSH (p<0.05) and lower the adherence ability (p<0.003) towards experimental pellicles. CONCLUSION: Combination between Gt and Sp aqueous extracts exhibited synergistic anti-plaque activity, and could be used as a useful active agent to produce oral health care products. [Mh] Termos MeSH primário: Actinomyces viscosus/efeitos dos fármacos Placa Dentária/microbiologia Extratos Vegetais/farmacologia Salvadoraceae/química Streptococcus mitis/efeitos dos fármacos Streptococcus sanguis/efeitos dos fármacos Chá/química [Mh] Termos MeSH secundário: Actinomyces viscosus/fisiologia Antibacterianos/farmacologia Aderência Bacteriana/efeitos dos fármacos Biofilmes/efeitos dos fármacos Película Dentária/microbiologia Placa Dentária/tratamento farmacológico Sinergismo Farmacológico Testes de Sensibilidade Microbiana Saliva/química Streptococcus mitis/fisiologia Streptococcus sanguis/fisiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Plant Extracts); 0 (Tea) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170813 [Lr] Data última revisão: 170813 [Sb] Subgrupo de revista: D; IM [Da] Data de entrada para processamento: 160626 [St] Status: MEDLINE

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[PMID]: 27325438 [Au] Autor: Rasmussen LH; Dargis R; Højholt K; Christensen JJ; Skovgaard O; Justesen US; Rosenvinge FS; Moser C; Lukjancenko O; Rasmussen S; Nielsen XC [Ad] Endereço: Department of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark. [Ti] Título: Whole genome sequencing as a tool for phylogenetic analysis of clinical strains of Mitis group streptococci. [So] Source: Eur J Clin Microbiol Infect Dis;35(10):1615-25, 2016 Oct. [Is] ISSN: 1435-4373 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Identification of Mitis group streptococci (MGS) to the species level is challenging for routine microbiology laboratories. Correct identification is crucial for the diagnosis of infective endocarditis, identification of treatment failure, and/or infection relapse. Eighty MGS from Danish patients with infective endocarditis were whole genome sequenced. We compared the phylogenetic analyses based on single genes (recA, sodA, gdh), multigene (MLSA), SNPs, and core-genome sequences. The six phylogenetic analyses generally showed a similar pattern of six monophyletic clusters, though a few differences were observed in single gene analyses. Species identification based on single gene analysis showed their limitations when more strains were included. In contrast, analyses incorporating more sequence data, like MLSA, SNPs and core-genome analyses, provided more distinct clustering. The core-genome tree showed the most distinct clustering. [Mh] Termos MeSH primário: Variação Genética Genoma Bacteriano Filogenia Análise de Sequência de DNA Streptococcus mitis/classificação Streptococcus mitis/genética [Mh] Termos MeSH secundário: Análise por Conglomerados Dinamarca Endocardite/microbiologia Seres Humanos Estudos Retrospectivos Infecções Estreptocócicas/microbiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160622 [St] Status: MEDLINE [do] DOI: 10.1007/s10096-016-2700-2

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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