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[PMID]:29458668
[Au] Autor:Al-Ahmad A; Muzafferiy F; Anderson AC; Wölber JP; Ratka-Krüger P; Fretwurst T; Nelson K; Vach K; Hellwig E
[Ad] Endereço:1​Department of Operative Dentistry and Periodontology, Faculty of Medicine, Medical Center - University of Freiburg, Germany.
[Ti] Título:Shift of microbial composition of peri-implantitis-associated oral biofilm as revealed by 16S rRNA gene cloning.
[So] Source:J Med Microbiol;67(3):332-340, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Micro-organisms are important triggers of peri-implant inflammation and analysing their diversity is necessary for peri-implantitis treatment. This study aimed to analyse and compare the microbiota associated with individuals with peri-implantitis, as well as clinically healthy implant sites. METHODOLOGY: Subgingival biofilm samples were taken from 10 individuals with peri-implantitis and from at least 1 clinically healthy implant. DNA was extracted and bacterial 16S rRNA genes were amplified using universal primers. After cloning the PCR-products, amplified inserts of positive clones were digested using restriction endonucleases, and the chosen clones were sequenced. The 16S rDNA-sequences were compared to those from the public sequence databases GenBank, EMBL and DDBJ to determine the corresponding taxa. RESULTS: Differing distributions of taxa belonging to the phyla Firmicutes, Bacteroidetes, Fusobacteria, Actinobacteria, Proteobacteria, Synergistetes, Spirochaetae and TM 7 were detected in both the healthy implant (HI) and the peri-implantitis (PI) groups. A significantly higher relative abundance of phylum Bacteroidetes, as well as of the species Fusobacterium nucleatum, were found in the PI group (P<0.05). The putative periodontal red complex (Porphyromonas gingivalis, Tannerella forsythia) was also detected at significantly higher levels in the PI group (P<0.05), whereas the yellow group, as well as the species Veillonella dispar, tended to be associated with the HI group. CONCLUSION: A shift in the healthy subgingival microbiota was shown in peri-implantitis-associated biofilm. Anaerobic Gram-negative periopathogens, including P. gingivalis and T. forsythia, seem to play an important role in peri-implantitis development and should be considered in treatment and prevention strategies.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Biofilmes
Microbiota/genética
Peri-Implantite/microbiologia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Bactérias/classificação
Bactérias/genética
Carga Bacteriana
Fenômenos Fisiológicos Bacterianos
Bacteroides/genética
Bacteroides/isolamento & purificação
Feminino
Fusobacterium nucleatum/genética
Fusobacterium nucleatum/isolamento & purificação
Genes de RNAr
Gengiva/microbiologia
Seres Humanos
Masculino
Meia-Idade
Porphyromonas gingivalis/genética
Porphyromonas gingivalis/isolamento & purificação
Prevotella intermedia/genética
Prevotella intermedia/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000682


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[PMID]:29217301
[Au] Autor:Bruneau A; Baylatry MT; Joly AC; Sokol H
[Ad] Endereço:AP-HP, hôpital Saint-Antoine, pharmacie hospitaliere, 184, rue du faubourg Saint-Antoine, 75012 Paris, France.
[Ti] Título:[Gut microbiota: What impact on colorectal carcinogenesis and treatment?]
[Ti] Título:Le microbiote intestinal : quels impacts sur la carcinogenèse et le traitement du cancer colorectal ?.
[So] Source:Bull Cancer;105(1):70-80, 2018 Jan.
[Is] ISSN:1769-6917
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:The gut microbiota, composed of 10 microorganisms, is now considered as a "hidden organ", regarding to its digestive, metabolic and immune functions, which are helpful to its host. For the last 15 years, advances in molecular biology have highlighted the association of gut microbiota dysbiosis with several diseases, including colorectal cancer. An increased abundance of some bacteria (including Fusobacterium nucleatum, Bacteroides fragilis, Escherichia coli) is associated with cancer, whereas others seem to be protective (Faecalibacterium prausnitzii). Several mechanisms, which are species-specific, are involved in colorectal carcinogenesis. Most of the time, bacterial toxins are involved in pro-inflammatory processes and in activation of angiogenesis and cellular proliferation pathways. The identification of these bacteria leads to envisage the gut microbiota as potential screening tool for colorectal cancer. Recent studies showed a relation between the gut microbiota and the efficacy and toxicity of chemotherapies (oxaliplatin, irinotecan) and immunotherapies (including ipilimumab). Therapeutic approaches targeting the gut microbiota are now available (probiotics, fecal microbiota transplantation…). New therapeutic strategy combining both chemotherapy and/or immunotherapy with an adjuvant treatment targeting the gut microbiota can now be developed in order to improve treatment response and tolerance.
[Mh] Termos MeSH primário: Neoplasias Colorretais/microbiologia
Neoplasias Colorretais/terapia
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Toxinas Bacterianas/metabolismo
Bacteroides fragilis
Proliferação Celular
Resistência a Medicamentos Antineoplásicos
Disbiose/induzido quimicamente
Disbiose/complicações
Enterococcus faecalis
Escherichia coli
Faecalibacterium prausnitzii
Transplante de Microbiota Fecal
Fusobacterium nucleatum
Microbioma Gastrointestinal/fisiologia
Seres Humanos
Neovascularização Patológica/microbiologia
Probióticos/uso terapêutico
Streptococcus gallolyticus
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Toxins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


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[PMID]:29190775
[Au] Autor:Ahn SH; Chun S; Park C; Lee JH; Lee SW; Lee TH
[Ad] Endereço:Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
[Ti] Título:Transcriptome profiling analysis of senescent gingival fibroblasts in response to Fusobacterium nucleatum infection.
[So] Source:PLoS One;12(11):e0188755, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Periodontal disease is caused by dental plaque biofilms. Fusobacterium nucleatum is an important periodontal pathogen involved in the development of bacterial complexity in dental plaque biofilms. Human gingival fibroblasts (GFs) act as the first line of defense against oral microorganisms and locally orchestrate immune responses by triggering the production of reactive oxygen species and pro-inflammatory cytokines (IL-6 and IL-8). The frequency and severity of periodontal diseases is known to increase in elderly subjects. However, despite several studies exploring the effects of aging in periodontal disease, the underlying mechanisms through which aging affects the interaction between F. nucleatum and human GFs remain unclear. To identify genes affected by infection, aging, or both, we performed an RNA-Seq analysis using GFs isolated from a single healthy donor that were passaged for a short period of time (P4) 'young GFs' or for longer period of time (P22) 'old GFs', and infected or not with F. nucleatum. Comparing F. nucleatum-infected and uninfected GF(P4) cells the differentially expressed genes (DEGs) were involved in host defense mechanisms (i.e., immune responses and defense responses), whereas comparing F. nucleatum-infected and uninfected GF(P22) cells the DEGs were involved in cell maintenance (i.e., TGF-ß signaling, skeletal development). Most DEGs in F. nucleatum-infected GF(P22) cells were downregulated (85%) and were significantly associated with host defense responses such as inflammatory responses, when compared to the DEGs in F. nucleatum-infected GF(P4) cells. Five genes (GADD45b, KLF10, CSRNP1, ID1, and TM4SF1) were upregulated in response to F. nucleatum infection; however, this effect was only seen in GF(P22) cells. The genes identified here appear to interact with each other in a network associated with free radical scavenging, cell cycle, and cancer; therefore, they could be potential candidates involved in the aged GF's response to F. nucleatum infection. Further studies are needed to confirm these observations.
[Mh] Termos MeSH primário: Fusobacterium nucleatum/patogenicidade
Perfilação da Expressão Gênica
Gengiva/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Células Cultivadas
Senescência Celular
Fibroblastos/metabolismo
Fibroblastos/microbiologia
Gengiva/citologia
Gengiva/microbiologia
Seres Humanos
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188755


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[PMID]:29016688
[Au] Autor:Jia YP; Wang K; Zhang ZJ; Tong YN; Han D; Hu CY; Li Q; Xiang Y; Mao XH; Tang B
[Ad] Endereço:Department of Clinical Microbiology and Immunology, Southwest Hospital & College of Medical Laboratory Science, Third Military Medical University, Chongqing, China.
[Ti] Título:TLR2/TLR4 activation induces Tregs and suppresses intestinal inflammation caused by Fusobacterium nucleatum in vivo.
[So] Source:PLoS One;12(10):e0186179, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Toll-like receptors (TLRs) 2 and 4 play critical roles in intestinal inflammation caused by Fusobacterium nucleatum (F. nucleatum) infection, but the role of TLR2/TLR4 in regulation of proinflammatory cytokines remains unknown. In this study, through microarray analysis and qRT-PCR, we showed that TLR2/TLR4 are involved in the F. nucleatum-induced inflammatory signaling pathway in Caco-2 cells, C57BL/6 mice and human clinical specimens. In TLR2-/- and TLR4-/- mice, F. nucleatum infection resulted in increased colonization of the bacteria and production of the proinflammatory cytokines IL-8, IL-1ß and TNF-α. In addition, the ratio of Foxp3+ CD4+ T cells in the total CD4+ T cells in TLR2-/- and TLR4-/- mice was less than that in wild-type mice, and the ratio in hybrid mice was more than that in knockout mice, which suggested that TLR2/TLR4 mediated the number of Tregs. Furthermore, it was observed that inflammatory cytokine levels were reduced in TLR2-/- mice after Treg transfer. Thus, these data indicate that TLR2/TLR4 regulate F. nucleatum-induced inflammatory cytokines through Tregs in vivo.
[Mh] Termos MeSH primário: Infecções por Fusobacterium/imunologia
Inflamação/imunologia
Receptor 2 Toll-Like/genética
Receptor 4 Toll-Like/genética
[Mh] Termos MeSH secundário: Adulto
Animais
Células CACO-2
Feminino
Infecções por Fusobacterium/microbiologia
Infecções por Fusobacterium/patologia
Fusobacterium nucleatum/imunologia
Fusobacterium nucleatum/patogenicidade
Seres Humanos
Inflamação/genética
Inflamação/microbiologia
Intestinos/microbiologia
Intestinos/patologia
Masculino
Camundongos
Camundongos Knockout
Análise em Microsséries
Meia-Idade
Transdução de Sinais
Linfócitos T Reguladores/imunologia
Receptor 2 Toll-Like/imunologia
Receptor 4 Toll-Like/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tlr2 protein, mouse); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 2); 0 (Toll-Like Receptor 4)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186179


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[PMID]:28833079
[Au] Autor:Eklöf V; Löfgren-Burström A; Zingmark C; Edin S; Larsson P; Karling P; Alexeyev O; Rutegård J; Wikberg ML; Palmqvist R
[Ad] Endereço:Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden.
[Ti] Título:Cancer-associated fecal microbial markers in colorectal cancer detection.
[So] Source:Int J Cancer;141(12):2528-2536, 2017 Dec 15.
[Is] ISSN:1097-0215
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Colorectal cancer (CRC) is the second most common cause of cancer death in the western world. An effective screening program leading to early detection of disease would severely reduce the mortality of CRC. Alterations in the gut microbiota have been linked to CRC, but the potential of microbial markers for use in CRC screening has been largely unstudied. We used a nested case-control study of 238 study subjects to explore the use of microbial markers for clbA+ bacteria harboring the pks pathogenicity island, afa-C+ diffusely adherent Escherichia coli harboring the afa-1 operon, and Fusobacterium nucleatum in stool as potential screening markers for CRC. We found that individual markers for clbA+ bacteria and F. nucleatum were more abundant in stool of patients with CRC, and could predict cancer with a relatively high specificity (81.5% and 76.9%, respectively) and with a sensitivity of 56.4% and 69.2%, respectively. In a combined test of clbA+ bacteria and F. nucleatum, CRC was detected with a specificity of 63.1% and a sensitivity of 84.6%. Our findings support a potential value of microbial factors in stool as putative noninvasive biomarkers for CRC detection. We propose that microbial markers may represent an important future screening strategy for CRC, selecting patients with a "high-risk" microbial pattern to other further diagnostic procedures such as colonoscopy.
[Mh] Termos MeSH primário: Neoplasias Colorretais/diagnóstico
Fezes/microbiologia
Fusobacterium nucleatum/isolamento & purificação
Fatores de Virulência/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Proteínas de Bactérias/genética
Estudos de Casos e Controles
Neoplasias Colorretais/microbiologia
Detecção Precoce de Câncer
Feminino
Seres Humanos
Masculino
Programas de Rastreamento/métodos
Meia-Idade
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Virulence Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1002/ijc.31011


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[PMID]:28797303
[Au] Autor:Heyman L; Houri-Haddad Y; Heyman SN; Ginsburg I; Gleitman Y; Feuerstein O
[Ad] Endereço:Department of Prosthodontics, Hebrew University-Hadassah Faculty of Dental Medicine, P.O.B. 12272, 91120, Jerusalem, Israel.
[Ti] Título:Combined antioxidant effects of Neem extract, bacteria, red blood cells and Lysozyme: possible relation to periodontal disease.
[So] Source:BMC Complement Altern Med;17(1):399, 2017 Aug 10.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The common usage of chewing sticks prepared from Neem tree (Azadirachta indica) in India suggests its potential efficacy in periodontal diseases. The objective of this study is to explore the antibacterial effects of Neem leaf extract on the periodontophatic bacteria Porphyromonas gingivalis and Fusobacterium nucleatum, and its antioxidant capacities alone and in combination with bacteria and polycationic peptides that may be at the site of inflammation. METHODS: Neem leaf extract was prepared by ethanol extraction. The growth kinetics of P. gingivalis and F. nucleatum under anaerobic conditions in the presence of Neem leaf extract were measured. Broth microdilution test was used to determine the Minimal Inhibitory Concentration (MIC) of Neem leaf extract against each bacterial strain. The effect of Neem leaf extract on the coaggregation of the bacteria was assessed by a visual semi-quantitative assay. The antioxidant capacities of Neem leaf extract alone and in combination with bacteria, with the addition of red blood cells or the polycationic peptides chlorhexidine and lisozyme, were determined using a chemiluminescence assay. RESULTS: Neem leaf extract showed prominent dose-dependent antibacterial activity against P. gingivalis, however, had no effect on the growth of F. nucleatum nor on the coaggregation of the two bacteria. Yet, it showed intense antioxidant activity, which was amplified following adherence to bacteria and with the addition of red blood cells or the polycationic peptides. CONCLUSIONS: Neem leaf extract, containing polyphenols that adhere to oral surfaces, have the potential to provide long-lasting antibacterial as well as synergic antioxidant activities when in complex with bacteria, red blood cells and lisozyme. Thus, it might be especially effective in periodontal diseases.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antioxidantes/farmacologia
Azadirachta/química
Eritrócitos
Muramidase/metabolismo
Doenças Periodontais/microbiologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Anti-Infecciosos Locais
Clorexidina
Fusobacterium/efeitos dos fármacos
Fusobacterium nucleatum/efeitos dos fármacos
Fusobacterium nucleatum/crescimento & desenvolvimento
Seres Humanos
Índia
Medicina Tradicional
Testes de Sensibilidade Microbiana
Peptídeos
Doenças Periodontais/tratamento farmacológico
Doenças Periodontais/metabolismo
Fitoterapia
Folhas de Planta
Poliaminas
Polifenóis/farmacologia
Porphyromonas/efeitos dos fármacos
Porphyromonas gingivalis/efeitos dos fármacos
Porphyromonas gingivalis/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents, Local); 0 (Antioxidants); 0 (Peptides); 0 (Plant Extracts); 0 (Polyamines); 0 (Polyphenols); 0 (polycations); EC 3.2.1.17 (Muramidase); R4KO0DY52L (Chlorhexidine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1900-3


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[PMID]:28753429
[Au] Autor:Yu T; Guo F; Yu Y; Sun T; Ma D; Han J; Qian Y; Kryczek I; Sun D; Nagarsheth N; Chen Y; Chen H; Hong J; Zou W; Fang JY
[Ad] Endereço:State Key Laboratory for Oncogenes and Related Genes, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Cancer Institute,Shanghai Institute of Digestive Disea
[Ti] Título:Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy.
[So] Source:Cell;170(3):548-563.e16, 2017 Jul 27.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gut microbiota are linked to chronic inflammation and carcinogenesis. Chemotherapy failure is the major cause of recurrence and poor prognosis in colorectal cancer patients. Here, we investigated the contribution of gut microbiota to chemoresistance in patients with colorectal cancer. We found that Fusobacterium (F.) nucleatum was abundant in colorectal cancer tissues in patients with recurrence post chemotherapy, and was associated with patient clinicopathological characterisitcs. Furthermore, our bioinformatic and functional studies demonstrated that F. nucleatum promoted colorectal cancer resistance to chemotherapy. Mechanistically, F. nucleatum targeted TLR4 and MYD88 innate immune signaling and specific microRNAs to activate the autophagy pathway and alter colorectal cancer chemotherapeutic response. Thus, F. nucleatum orchestrates a molecular network of the Toll-like receptor, microRNAs, and autophagy to clinically, biologically, and mechanistically control colorectal cancer chemoresistance. Measuring and targeting F. nucleatum and its associated pathway will yield valuable insight into clinical management and may ameliorate colorectal cancer patient outcomes.
[Mh] Termos MeSH primário: Autofagia
Neoplasias Colorretais/tratamento farmacológico
Neoplasias Colorretais/patologia
Fusobacterium nucleatum/fisiologia
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/uso terapêutico
Capecitabina/uso terapêutico
Neoplasias Colorretais/metabolismo
Resistência a Medicamentos Antineoplásicos
Xenoenxertos
Camundongos
MicroRNAs/metabolismo
Transplante de Neoplasias
Compostos de Platina/uso terapêutico
Recidiva
Receptores Toll-Like/metabolismo
Microambiente Tumoral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (MIRN18A microRNA, human); 0 (MIRN4802 microRNA, human); 0 (MicroRNAs); 0 (Platinum Compounds); 0 (Toll-Like Receptors); 6804DJ8Z9U (Capecitabine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE


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[PMID]:28753421
[Au] Autor:Ramos A; Hemann MT
[Ad] Endereço:Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
[Ti] Título:Drugs, Bugs, and Cancer: Fusobacterium nucleatum Promotes Chemoresistance in Colorectal Cancer.
[So] Source:Cell;170(3):411-413, 2017 07 27.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The tumor microenvironment has recently been shown to play decisive roles in chemotherapeutic response. In this issue of Cell, Yu et al. add to these findings by identifying the bacterium Fusobacterium nucleatum as a previously unrecognized chemoresistance mediator in colorectal cancer, thereby establishing the microbiota as a potential therapeutic target.
[Mh] Termos MeSH primário: Neoplasias Colorretais/microbiologia
Fusobacterium nucleatum
[Mh] Termos MeSH secundário: Seres Humanos
Microbiota
Microambiente Tumoral
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE


  9 / 1268 MEDLINE  
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[PMID]:28633639
[Au] Autor:Jayasimhan D; Wu L; Huggan P
[Ad] Endereço:Department of Medicine, Waikato Hospital, Level 2 Waiora Waikato Building, Pembroke Street, Hamilton, 3204, New Zealand. d.jayasimhan@gmail.com.
[Ti] Título:Fusobacterial liver abscess: a case report and review of the literature.
[So] Source:BMC Infect Dis;17(1):440, 2017 Jun 20.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Fusobacteriae are facultative anaerobic gram-negative bacilli which cause a range of invasive infections, amongst which pyogenic liver abscesses are rare. We describe a case of Fusobacterium nucleatum liver abscess and review the relevant literature. CASE PRESENTATION: A 51-year-old lady presented with a 4-day history of abdominal pain, diarrhoea, fever, rigors, and lethargy. Imaging revealed an abscess which was drained. Cultures of the blood and abscess aspirate grew Fusobacterium nucleatum and Prevotella pleuritidis respectively. She achieved full recovery following treatment. A MEDLINE search was undertaken using free-text and Medical Subject Headings (MeSH), keywords "Fusobacterium" and "Liver abscess". Non-English language reports and cases without confirmed growth of Fusobacterium species were excluded. Additional cases were identified by surveying the references of each report and by using the same keywords in a web-based search. Forty-eight cases were identified, 41 in men. The median age was 42.5, with an interquartile range of 33. F. nucleatum and F. necrophorum were in involved in 22 cases each, and 4 cases were not further speciated. Among cases of F. nucleatum liver abscess, nine were attributed to periodontal disease, four to lower gastrointestinal tract disease, one to Lemierre's Syndrome, and eight were considered cryptogenic. All patients treated made a full recovery. Antimicrobial treatment duration ranged from 2 weeks to 6 months with a median of 6 weeks. CONCLUSION: Fusobacterium nucleatum is an uncommon cause of liver abscess generally associated with good clinical outcomes with contemporary medical and surgical care.
[Mh] Termos MeSH primário: Infecções por Fusobacterium/etiologia
Fusobacterium nucleatum/patogenicidade
Abscesso Hepático Piogênico/microbiologia
[Mh] Termos MeSH secundário: Infecções por Bacteroidaceae/etiologia
Drenagem
Feminino
Infecções por Fusobacterium/tratamento farmacológico
Infecções por Fusobacterium/microbiologia
Seres Humanos
Abscesso Hepático Piogênico/tratamento farmacológico
Abscesso Hepático Piogênico/etiologia
Masculino
Meia-Idade
Doenças Periodontais/microbiologia
Prevotella/patogenicidade
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2548-9


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[PMID]:28628741
[Au] Autor:Jiang YS; Stacy A; Whiteley M; Ellington AD; Bhadra S
[Ad] Endereço:Department of Molecular Biosciences, College of Natural Sciences, The University of Texas, Austin, TX, 78712, USA.
[Ti] Título:Amplicon Competition Enables End-Point Quantitation of Nucleic Acids Following Isothermal Amplification.
[So] Source:Chembiochem;18(17):1692-1695, 2017 Sep 05.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:It is inherently difficult to quantitate nucleic acid analytes with most isothermal amplification assays. We developed loop-mediated isothermal amplification (LAMP) reactions in which competition between defined numbers of "false" and "true" amplicons leads to order of magnitude quantitation by a single endpoint determination. These thresholded LAMP reactions were successfully used to directly and quantitatively estimate the numbers of nucleic acids in complex biospecimens, including directly from cells and in sewage, with the values obtained closely correlating with qPCR quantitations. Thresholded LAMP reactions are amenable to endpoint readout by cell phone, unlike other methods that require continuous monitoring, and should therefore prove extremely useful in developing one-pot reactions for point-of-care diagnostics without needing sophisticated material or informatics infrastructure.
[Mh] Termos MeSH primário: Técnicas de Amplificação de Ácido Nucleico
Ácidos Nucleicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Infecções por Fusobacterium/diagnóstico
Fusobacterium nucleatum/genética
Camundongos
Neuropilina-2/genética
Neuropilina-2/metabolismo
Ácidos Nucleicos/genética
Sistemas Automatizados de Assistência Junto ao Leito
Proteínas Proto-Oncogênicas B-raf/genética
Proteínas Proto-Oncogênicas B-raf/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Neuropilin-2); 0 (Nucleic Acids); 0 (RNA, Messenger); EC 2.7.11.1 (Braf protein, mouse); EC 2.7.11.1 (Proto-Oncogene Proteins B-raf)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700317



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