Base de dados : MEDLINE
Pesquisa : B03.440.040 [Categoria DeCS]
Referências encontradas : 191 [refinar]
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  1 / 191 MEDLINE  
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[PMID]:29231658
[Au] Autor:Frivik JO; Noraas S; Grankvist A; Wennerås C; Quarsten H
[Ti] Título:A man in his sixties from Southern Norway with intermittent fever.
[Ti] Título:En mann i 60-årene fra Sørlandet med intermitterende feber..
[So] Source:Tidsskr Nor Laegeforen;137(23-24), 2017 12 12.
[Is] ISSN:0807-7096
[Cp] País de publicação:Norway
[La] Idioma:eng; nor
[Mh] Termos MeSH primário: Infecções por Anaplasmataceae
Febre/virologia
Doenças Transmitidas por Carrapatos
[Mh] Termos MeSH secundário: Idoso
Anaplasmataceae/isolamento & purificação
Infecções por Anaplasmataceae/complicações
Infecções por Anaplasmataceae/diagnóstico
Infecções por Anaplasmataceae/tratamento farmacológico
Antibacterianos/uso terapêutico
Astenia/virologia
Doxiciclina/uso terapêutico
Seres Humanos
Masculino
Meia-Idade
Noruega
Doenças Transmitidas por Carrapatos/complicações
Doenças Transmitidas por Carrapatos/diagnóstico
Doenças Transmitidas por Carrapatos/tratamento farmacológico
[Pt] Tipo de publicação:CASE REPORTS
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); N12000U13O (Doxycycline)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.4045/tidsskr.17.0353


  2 / 191 MEDLINE  
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[PMID]:27902186
[Au] Autor:Cicala F; Moore JD; Cáceres-Martínez J; Del Río-Portilla MA; Hernández-Rodríguez M; Vásquez-Yeomans R; Rocha-Olivares A
[Ad] Endereço:1​Molecular Ecology Laboratory, Department of Biological Oceanography, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada Baja California 22860, Mexico.
[Ti] Título:Multigenetic characterization of 'Candidatus Xenohaliotis californiensis'.
[So] Source:Int J Syst Evol Microbiol;67(1):42-49, 2017 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:'Candidatus Xenohaliotis californiensis' (or Ca.Xc) is the aetiological agent of withering syndrome, a chronic wasting disease affecting most if not all North American species of abalone, and has been described as a Rickettsiales-like prokaryote. Genetic data regarding this species are limited to the 16S rRNA gene. The inability to grow it axenically has hindered its genetic and genomic characterization and, in consequence, a thorough analysis of its systematics. Here, we amplified and sequenced five genes (16S rRNA, 23S rRNA, ftsZ, virD4 and virB11) of Ca.Xc from infected abalone to analyse its phylogenetic position. Phylogenies from concatenated DNA and amino acid sequences with representative genera of most Rickettsiales unequivocally place Ca.Xc in the family Anaplasmataceae. Furthermore, the family has two reciprocally monophyletic lineages: one leading to (Neorickettsia, Ca.Xc) and the other to ((Ehrlichia, Anaplasma), Wolbachia)). A molecular-clock Bayesian reconstruction places Ca.Xc as the most basal lineage in Anaplasmataceae. These phylogenetic hypotheses shed light on patterns of host evolution and of ecological transitions. Specifically, Neorickettsia and Ca.Xc inhabit aquatic hosts whereas the remaining Anaplasmataceae are found in terrestrial hosts. Additionally, our evolutionary timeline places the directly transmitted marine Ca.Xc as the basal Anaplasmataceae, ancestral to both freshwater and terrestrial species with adaptations leading to more complex life cycles involving intermediate vectors or reservoir species; this supports the hypothesis of a marine origin for this bacterial family.
[Mh] Termos MeSH primário: Anaplasmataceae/classificação
Gastrópodes/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Anaplasmataceae/genética
Animais
Técnicas de Tipagem Bacteriana
Teorema de Bayes
DNA Bacteriano/genética
Genes Bacterianos
Funções Verossimilhança
Modelos Genéticos
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001563


  3 / 191 MEDLINE  
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[PMID]:26876304
[Au] Autor:Hodzic A; Mitkovà B; Modrý D; Juránková J; Frgelecová L; Forejtek P; Steinbauer V; Duscher GG
[Ad] Endereço:Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
[Ti] Título:A new case of the enigmatic Candidatus Neoehrlichia sp. (FU98) in a fox from the Czech Republic.
[So] Source:Mol Cell Probes;31:59-60, 2017 Feb.
[Is] ISSN:1096-1194
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study reports a new case of Candidatus Neoehrlichia sp. (FU98) infection in a fox from the Czech Republic, and provides confirmatory evidence on the occurrence of this newly identified sequence type. However, further studies are needed to investigate the distribution, host range and possible vector(s) for this bacterium, as well as its impact on animals and humans.
[Mh] Termos MeSH primário: Anaplasmataceae/fisiologia
Raposas/microbiologia
[Mh] Termos MeSH secundário: Animais
República Tcheca
DNA Bacteriano/sangue
DNA Bacteriano/genética
Raposas/sangue
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160216
[St] Status:MEDLINE


  4 / 191 MEDLINE  
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[PMID]:28151609
[Au] Autor:Karbowiak G; Biernat B; Stanczak J; Werszko J; Wróblewski P; Szewczyk T; Sytykiewicz H
[Ad] Endereço:W. Stefanski Institute of Parasitology, Polish Academy of Sciences, ul. Twarda 51/55, 00-818 Warszawa, Poland
[Ti] Título:The role of particular ticks developmental stages in the circulation of tick-borne pathogens in Central Europe. 4. Anaplasmataceae
[So] Source:Ann Parasitol;62(4):267-284, 2016.
[Is] ISSN:2299-0631
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:In Central European conditions, two species of Anaplasmataceae have epidemiological significance ­ Candidatus Neoehrlichia micurensis and Anaplasma phagocytophilum. Tick Ixodes ricinus is considered as their main vector, wild mammals as the animal reservoir. There is presented the transstadial transmission in ticks, due to the lack of transovarial mode the circulation goes mainly between immature ticks and hosts; pathogen circulates primarily in the cycle: infected rodent → the tick larva → the nymph → the mammal reservoir → the larva of the tick. The tick stages able to effectively infect human are nymphs and adult females, males do not participate in the follow transmission. The summary of available data of different A. phagocytophilum strains associations with different hosts revealed at least few distinct enzootic cycle, concern the same ticks species and different mammal hosts. It is possible to reveal in Central Europe the existence of at least three different epidemiological transmission cycles of A. phagocytophilum. The first cycle involves strains pathogenic for human and identical strains from horses, dogs, cats, wild boars, hedgehogs, possibly red foxes. The second cycle involves deer, European bison and possibly domestic ruminants. The third cycle contains strains from voles, shrew and possibly Apodemus mice. In Western Europe voles might be involved in separate enzootic cycle with Ixodes trianguliceps as the vector.
[Mh] Termos MeSH primário: Infecções por Anaplasmataceae/veterinária
Anaplasmataceae/classificação
Doenças Transmitidas por Carrapatos/microbiologia
Carrapatos/microbiologia
[Mh] Termos MeSH secundário: Infecções por Anaplasmataceae/epidemiologia
Infecções por Anaplasmataceae/parasitologia
Infecções por Anaplasmataceae/transmissão
Animais
Europa (Continente)/epidemiologia
Seres Humanos
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170602
[Lr] Data última revisão:
170602
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170206
[St] Status:MEDLINE
[do] DOI:10.17420/ap6204.62


  5 / 191 MEDLINE  
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[PMID]:27747194
[Au] Autor:Cangi N; Gordon JL; Bournez L; Pinarello V; Aprelon R; Huber K; Lefrançois T; Neves L; Meyer DF; Vachiéry N
[Ad] Endereço:CIRAD, UMR CMAEEPetit-Bourg, Guadeloupe, France; INRA, UMR1309 CMAEEMontpellier, France; Centro de Biotecnologia-UEM, Eduardo Mondlane UniversityMaputo, Mozambique; Université des AntillesPointe-à-Pitre, Guadeloupe, France.
[Ti] Título:Recombination Is a Major Driving Force of Genetic Diversity in the Anaplasmataceae .
[So] Source:Front Cell Infect Microbiol;6:111, 2016.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The disease, Heartwater, caused by the , represents a major problem for tropical livestock and wild ruminants. Up to now, no effective vaccine has been available due to a limited cross protection of vaccinal strains on field strains and a high genetic diversity of within geographical locations. To address this issue, we inferred the genetic diversity and population structure of 194 isolates circulating worldwide using Multilocus Sequence Typing based on , and . Phylogenetic trees and networks were generated using BEAST and SplitsTree, respectively, and recombination between the different genetic groups was tested using the PHI test for recombination. Our study reveals the repeated occurrence of recombination between strains, suggesting that it may occur frequently in the genome and has likely played an important role in the maintenance of genetic diversity and the evolution of . Despite the unclear phylogeny and phylogeography, isolates are clustered into two main groups: Group 1 (West Africa) and a Group 2 (worldwide) which is represented by West, East, and Southern Africa, Indian Ocean, and Caribbean strains. Some sequence types are common between West Africa and Caribbean and between Southern Africa and Indian Ocean strains. These common sequence types highlight two main introduction events due to the movement of cattle: from West Africa to Caribbean and from Southern Africa to the Indian Ocean islands. Due to the long branch lengths between Group 1 and Group 2, and the propensity for recombination between these groups, it seems that the West African clusters of Subgroup 2 arrived there more recently than the original divergence of the two groups, possibly with the original waves of domesticated ruminants that spread across the African continent several thousand years ago.
[Mh] Termos MeSH primário: Anaplasmataceae/genética
Ehrlichia ruminantium/genética
Evolução Molecular
Variação Genética/genética
Genótipo
Recombinação Genética
[Mh] Termos MeSH secundário: África/epidemiologia
Animais
Proteínas de Bactérias/genética
Sequência de Bases
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/microbiologia
DNA Bacteriano
Ehrlichia ruminantium/classificação
Ehrlichia ruminantium/isolamento & purificação
Ehrlichia ruminantium/patogenicidade
Hidropericárdio/sangue
Hidropericárdio/epidemiologia
Hidropericárdio/microbiologia
Ilhas do Oceano Índico/epidemiologia
Moçambique/epidemiologia
Tipagem de Sequências Multilocus/métodos
Filogenia
Reação em Cadeia da Polimerase/métodos
Superóxido Dismutase/genética
Carrapatos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (LipA protein, Bacteria); 0 (lipase activator protein, Bacteria); EC 1.15.1.1 (SodB protein, Bacteria); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE


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[PMID]:27707682
[Au] Autor:Hofmann-Lehmann R; Wagmann N; Meli ML; Riond B; Novacco M; Joekel D; Gentilini F; Marsilio F; Pennisi MG; Lloret A; Carrapiço T; Boretti FS
[Ad] Endereço:Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Switzerland.
[Ti] Título:Detection of 'Candidatus Neoehrlichia mikurensis' and other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean countries.
[Ti] Título:Nachweis von 'Candidatus Neoehrlichia mikurensis' und anderen Anaplasmataceae und Rickettsiaceae in Kaniden in der Schweiz und in Mittelmeerländern..
[So] Source:Schweiz Arch Tierheilkd;158(10):691-700, 2016 Oct.
[Is] ISSN:0036-7281
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: 'Candidatus Neoehrlichia mikurensis' is an emerging tick-borne zoonotic agent that primarily affects immunocompromised human patients. Dogs and foxes are frequently exposed to ticks, and both species are in close proximity to humans. This is the first study to systematically investigate the occurrence of 'Candidatus Neoehrlichia mikurensis' in Canidae in Europa. We analyzed 1'739 blood samples from dogs in Switzerland, Italy, Spain and Portugal and 162 blood samples from free-ranging red foxes (Vulpes vulpes) in Switzerland. All samples were tested using a previously described multiplex real-time PCR for the Anaplasmataceae family, the 'Candidatus Neoehrlichia' genus and the 'Candidatus Neoehrlichia mikurensis' species. All Anaplasmataceae positive samples were subsequently tested using specific real-time PCRs for Anaplasma phagocytophilum, Anaplasma platys, Ehrlichia canis and Rickettsia helvetica. Among the tested animals, one dog from Zurich tested positive for 'Candidatus Neoehrlichia mikurensis'. The 12-year old West Highland white terrier had been splenectomized 3 months prior to the blood collection and presented with polyuria/polydipsia. Fanconi syndrome was diagnosed based on glucosuria with normoglycemia and hyperaminoaciduria. A. platys and E. canis were detected in 14/249 dogs from Sicily and Portugal; two of the dogs were coinfected with both agents. Four Swiss foxes tested positive for A. phagocytophilium. R. helvetica was detected for the first time in a red fox. In conclusion, 'Candidatus Neoehrlichia mikurensis' infection should be considered in sick dogs, particularly when immunocompromised. The pathogen seems not to be widespread in Canidae in the investigated countries. Conversely, other Anaplasmataceae were more readily detected in dogs and foxes.
[Mh] Termos MeSH primário: Infecções por Anaplasmataceae/veterinária
Doenças do Cão/diagnóstico
Doenças do Cão/epidemiologia
Infecções por Rickettsiaceae/veterinária
Zoonoses/diagnóstico
Zoonoses/epidemiologia
[Mh] Termos MeSH secundário: Anaplasmataceae/isolamento & purificação
Infecções por Anaplasmataceae/diagnóstico
Infecções por Anaplasmataceae/epidemiologia
Infecções por Anaplasmataceae/microbiologia
Animais
Coinfecção
Doenças do Cão/microbiologia
Cães
Raposas/microbiologia
Genes Bacterianos/genética
Região do Mediterrâneo
Reação em Cadeia da Polimerase/veterinária
Prevalência
Rickettsiaceae/isolamento & purificação
Infecções por Rickettsiaceae/epidemiologia
Infecções por Rickettsiaceae/microbiologia
Suíça
Zoonoses/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161007
[St] Status:MEDLINE
[do] DOI:10.17236/sat00087


  7 / 191 MEDLINE  
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[PMID]:27468908
[Au] Autor:Gofton AW; Doggett S; Ratchford A; Ryan U; Irwin P
[Ad] Endereço:1​Vector and Water-Borne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia, Australia.
[Ti] Título:Phylogenetic characterisation of two novel Anaplasmataceae from Australian Ixodes holocyclus ticks: 'Candidatus Neoehrlichia australis' and 'Candidatus Neoehrlichia arcana'.
[So] Source:Int J Syst Evol Microbiol;66(10):4256-4261, 2016 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recently, two novel species of Anaplasmataceae were detected in the Australian paralysis tick, Ixodes holocyclus, by 16S rRNA gene metabarcoding. Analysis of these sequences suggested that these novel organisms are closely related to the genus 'Candidatus Neoehrlichia'. In this study, phylogenetic analysis of 16S rRNA (1264 bp), groESL (1047 bp) and gltA (561 bp) gene sequences, and concatenated (2872 bp) sequences, all concur that these novel species belong in the genus 'Candidatus Neoehrlichia' and are most closely related to, but distinct from the only other recognised members of this genus, 'Candidatus Neoehrlichia mikurensis' and 'Candidatus Neoehrlichia lotoris'. Based on their unique molecular signature, we propose to designate these species 'Candidatus Neoehrlichia australis' (reference strain HT41R) and 'Candidatus Neoehrlichia arcana' (reference strain HT94R). Identical 'Candidatus Neoehrlichia australis' 16S rRNA, groESL and gltA sequences were detected in 34/391 (8.7 %) individual Ixodes holocyclus ticks, and sequences were most similar to 'Candidatus Neoehrlichia lotoris' (96.2 %, 83.1 % and 67.2 %, respectively) and 'Candidatus Neoehrlichia mikurensis' (96.2 %, 84 % and 68.4 % respectively). Likewise, identical 'Candidatus Neoehrlichia arcana' 16S rRNA, groESL and gltA sequences were detected in 12/391 (3.1 %) Ixodes holocyclus ticks, and sequences were most similar to 'Candidatus Neoehrlichia lotoris' (98.5 %, 88.7 % and 79.3 %, respectively) and 'Candidatus Neoehrlichia mikurensis' (96.3 %, 84 % and 67.4 % respectively). These new species are the first Anaplasmataceae (except Wolbachia spp.) to be found to be endemic to Australia. The pathogenic consequences of these organisms are yet to be determined.
[Mh] Termos MeSH primário: Anaplasmataceae/classificação
Ixodes/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Anaplasmataceae/genética
Anaplasmataceae/isolamento & purificação
Animais
Austrália
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Genes Bacterianos
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160730
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001344


  8 / 191 MEDLINE  
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[PMID]:27329450
[Au] Autor:Król N; Obiegala A; Pfeffer M; Lonc E; Kiewra D
[Ad] Endereço:Department of Microbial Ecology and Environmental Protection, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland. nina.krol2@uwr.edu.pl.
[Ti] Título:Detection of selected pathogens in ticks collected from cats and dogs in the Wroclaw Agglomeration, South-West Poland.
[So] Source:Parasit Vectors;9(1):351, 2016 06 21.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tick-borne infections are no longer confined to rural areas, they are documented with increasing frequency in urban settlements across the world. They are known to cause diseases in humans as well as in their companion animals. METHODS: During a period of 2 years, from January 2013 until December 2014, ticks were collected from dogs and cats in 18 veterinary clinics in the Wroclaw Agglomeration, Poland. In total, 1455 ticks were found on 931 pets: 760 domestic dogs and 171 cats. For molecular examinations 127 I. ricinus ticks (115 females and 12 males) were randomly selected, all collected I. hexagonus (n = 137, 32 females, 98 nymphs, 7 larvae) and all collected D. reticulatus (n = 46, 31 females, 15 males) were taken. Ixodes ricinus and I. hexagonus ticks were tested for Rickettsia spp., Anaplasma phagocytophilum, Candidatus Neoehrlichia mikurensis and Babesia spp., while D. reticulatus ticks were investigated for Rickettsia spp. and Babesia spp. only. RESULTS: In total, 65.4 % I. ricinus ticks were infected with at least one pathogen. Over 50 % of I. ricinus were positive for Rickettsia spp. (R. helvetica and R. monacensis). The infection level with A. phagocytophilum was 21.3 %. DNA of Cand. N. mikurensis was detected in 8.1 % I. ricinus ticks. Interestingly only female ticks were infected. The prevalence of Babesia spp. was confirmed in 9.0 % of I. ricinus involving the species B. microti and B. venatorum. A total of nineteen double, one triple and two quadruple infections were found in I. ricinus ticks only. Almost 11 % of I. hexagonus ticks were positive for at least one of the tested pathogens. Rickettsia spp. infection was found in 2.2 %, while A. phagocytophilum was detected in 8.1 % of I. hexagonus ticks. Only one nymph was positive for Cand. N. mikurensis and none of I. hexagonus ticks harbored a Babesia spp. Over 60 % of D. reticulatus ticks were positive for rickettsial DNA, exclusively belonging to the species R. raoultii. CONCLUSION: The high tick infestation rates and the prevalence of pathogens found in these ticks demonstrate a serious level of encounter to tick-borne diseases in urban dogs in the Wroclaw area, and provide evidence that dogs and cats themselves may substantially contribute to the circulation of the ticks and pathogens in the urban area.
[Mh] Termos MeSH primário: Anaplasmataceae/isolamento & purificação
Babesia/isolamento & purificação
Doenças do Gato/parasitologia
Doenças do Cão/parasitologia
Ixodidae/microbiologia
Rickettsia/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Vetores Aracnídeos/microbiologia
Doenças do Gato/epidemiologia
Doenças do Gato/transmissão
Gatos
Doenças do Cão/epidemiologia
Doenças do Cão/transmissão
Cães
Feminino
Masculino
Polônia/epidemiologia
Infestações por Carrapato/epidemiologia
Infestações por Carrapato/parasitologia
Infestações por Carrapato/veterinária
População Urbana
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170222
[Lr] Data última revisão:
170222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160623
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-016-1632-0


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[PMID]:27325400
[Au] Autor:Rudolf I; Betásová L; Bischof V; Venclíková K; Blazejová H; Mendel J; Hubálek Z; Kosoy M
[Ad] Endereço:Institute of Vertebrate Biology, v.v.i., Academy of Sciences, Kvetna 8, CZ-603 65, Brno, Czech Republic. rudolf@ivb.cz.
[Ti] Título:Molecular survey of arthropod-borne pathogens in sheep keds (Melophagus ovinus), Central Europe.
[So] Source:Parasitol Res;115(10):3679-82, 2016 Oct.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In the study, we screened a total of 399 adult sheep keds (Melophagus ovinus) for the presence of RNA and DNA specific for arboviral, bacterial, and protozoan vector-borne pathogens. All investigated keds were negative for flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi, Rickettsia spp., Anaplasma phagocytophilum, "Candidatus Neoehrlichia mikurensis," and Babesia spp. All ked pools were positive for Bartonella DNA. The sequencing of the amplified fragments of the gltA and 16S-23S rRNA demonstrated a 100 % homology with Bartonella melophagi previously isolated from a sheep ked and from human blood in the USA. The identification of B. melophagi in sheep keds in Central Europe highlights needs extending a list of hematophagous arthropods beyond ticks and mosquitoes for a search of emerging arthropod-borne pathogens.
[Mh] Termos MeSH primário: Anaplasma phagocytophilum/isolamento & purificação
Anaplasmataceae/isolamento & purificação
Babesia/isolamento & purificação
Bartonella/isolamento & purificação
Dípteros/microbiologia
Rickettsia/isolamento & purificação
Doenças dos Ovinos/microbiologia
[Mh] Termos MeSH secundário: Anaplasma phagocytophilum/genética
Anaplasmataceae/genética
Animais
Arbovirus/genética
Arbovirus/isolamento & purificação
Babesia/genética
Bartonella/genética
República Tcheca/epidemiologia
Feminino
Masculino
Rickettsia/genética
Análise de Sequência de DNA/veterinária
Ovinos
Doenças dos Ovinos/epidemiologia
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160622
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-016-5175-2


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[PMID]:27270389
[Au] Autor:Liesner JM; Krücken J; Schaper R; Pachnicke S; Kohn B; Müller E; Schulze C; von Samson-Himmelstjerna G
[Ad] Endereço:Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag Str. 7-13, 14163 Berlin, Germany.
[Ti] Título:Vector-borne pathogens in dogs and red foxes from the federal state of Brandenburg, Germany.
[So] Source:Vet Parasitol;224:44-51, 2016 Jul 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Dirofilaria repens is endemic in eastern and southern European regions but was recently found in Germany in dogs, mosquitoes and one human patient. Since some of the positive dog and mosquito samples were collected in Brandenburg, it was aimed to systematically assess the prevalence of D. repens and other canine vector-borne pathogens in Brandenburg. Dog owners also received a questionnaire and were asked to provide more information about the dogs including travel history. In total, 1023 dog blood samples as well as 195 fox spleen and 179 fox blood samples were collected. DNA was analysed by PCR for the presence of filariae, piroplasms, anaplasmataceae and Rickettsia spp. Filariae were detected in six dogs (0.6%), two were positive for DNA from D. repens, two from Dirofilaria immitis and two from Acanthocheilonema reconditum. One of the D. repens positive dogs originated from an animal shelter in Brandenburg, but the origin of the other one remained unknown. Interestingly, both D. repens ITS-1 sequences showed 100% identity to a D. repens sample obtained from a Japanese woman that travelled in Europe and were 97% identical to a newly proposed species Dirofilaria sp. 'hongkongensis' described from Hong Kong. However, identity to other D. repens sequences from Thailand was considerably lower (81%). Identity of 12S rRNA and cytochrome oxidase I to D. repens samples from southern Europe was 99%. Due to the low number of Dirofilaria spp. positive dogs and since the origin of these was unknown, endemic occurrence of Dirofilaria in Brandenburg could not be confirmed. Anaplasma phagocytophilum was found in 15 dogs (1.5%), Candidatus Neoehrlichia mikurensis in three dogs (0.3%) and E. canis in one dog (0.1%), which was co-infected with D. repens. Rickettsia spp. were detected in 8 dogs (0.8%), seven were Rickettsia raoultii and one was Rickettsia felis. To the author's knowledge, R. raoultii DNA was detected for the first time in dogs in Germany in this study and Candidatus N. mikurensis for the second time. In spleen samples of red foxes with 47.5% a high prevalence of piroplasms was found. Sequencing of 11 samples identified 10 as Theileria annae. Despite the high prevalence of this pathogen in its reservoir host, it was absent in dog samples. In one dog (0.1%), Babesia canis was detected but there was no further information about the dog's origin. Evaluation of the questionnaire identified a high proportion of dogs (74.2%, n=233) which was not protected by ectoparasiticides. Moreover, 21.2% (n=236) of the dogs originated from inland or abroad shelters, and therefore might potentially come from areas endemic for dirofilariosis or babesiosis.
[Mh] Termos MeSH primário: Babesiose/parasitologia
Doenças do Cão/parasitologia
Filariose/veterinária
Raposas/microbiologia
Raposas/parasitologia
Infecções por Bactérias Gram-Negativas/veterinária
[Mh] Termos MeSH secundário: Anaplasmataceae/genética
Anaplasmataceae/isolamento & purificação
Animais
Vetores Artrópodes/microbiologia
Vetores Artrópodes/parasitologia
Babesia/genética
Babesia/isolamento & purificação
Babesiose/epidemiologia
DNA Espaçador Ribossômico/genética
Doenças do Cão/epidemiologia
Cães
Complexo IV da Cadeia de Transporte de Elétrons/genética
Filariose/epidemiologia
Filariose/parasitologia
Filarioidea/genética
Filarioidea/isolamento & purificação
Alemanha/epidemiologia
Infecções por Bactérias Gram-Negativas/epidemiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Seres Humanos
RNA Ribossômico/genética
Rickettsia/genética
Rickettsia/isolamento & purificação
Homologia de Sequência do Ácido Nucleico
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal); 0 (RNA, ribosomal, 12S); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE



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