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[PMID]:28455331
[Au] Autor:Schötta AM; Wijnveld M; Stockinger H; Stanek G
[Ad] Endereço:Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria anna-margarita.schoetta@meduniwien.ac.at.
[Ti] Título:Approaches for Reverse Line Blot-Based Detection of Microbial Pathogens in Ixodes ricinus Ticks Collected in Austria and Impact of the Chosen Method.
[So] Source:Appl Environ Microbiol;83(13), 2017 Jul 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ticks transmit a large number of pathogens capable of causing human disease. In this study, the PCR-reverse line blot (RLB) method was used to screen for pathogens in a total of 554 ticks collected from all provinces of Austria. These pathogens belong to the genera , , / (including " Neoehrlichia"), , and The pathogens with the highest detected prevalence were spirochetes of the complex, in 142 ticks (25.6%). (80/142) was the most frequently detected species, followed by (38/142) and (36/142). , , and were found in 28 ticks, 5 ticks, and 1 tick, respectively. spp. were detected in 93 ticks (16.8%): (39/93), (38/93), (2/93), and (1/93). Thirteen samples remain uncharacterized. " Neoehrlichia mikurensis," spp. ( , , ), and were found in 4.5%, 2.7%, and 0.7%, respectively. was not detected. Multiple microorganisms were detected in 40 ticks (7.2%), and the cooccurrence of spp. and " Neoehrlichia mikurensis" showed a significant positive correlation. We also compared different PCR-RLBs for detection of and spp. and showed that different detection approaches provide highly diverse results, indicating that analysis of environmental samples remains challenging. This study determined the wide spectrum of tick-borne bacterial and protozoal pathogens that can be encountered in Austria. Surveillance of (putative) pathogenic microorganisms occurring in the environment is of medical importance, especially when those agents can be transmitted by ticks and cause disease. The observation of significant coinfections of certain microorganisms in field-collected ticks is an initial step to an improved understanding of microbial interactions in ticks. In addition, we show that variations in molecular detection methods, such as in primer pairs and target genes, can considerably influence the final results. For instance, detection of certain genospecies of borreliae may be better or worse by one method or the other, a fact of great importance for future screening studies.
[Mh] Termos MeSH primário: Anaplasma/isolamento & purificação
Southern Blotting/métodos
Borrelia/isolamento & purificação
Ixodes/microbiologia
Reação em Cadeia da Polimerase/métodos
Rickettsia/isolamento & purificação
[Mh] Termos MeSH secundário: Anaplasma/classificação
Anaplasma/genética
Animais
Áustria
Borrelia/classificação
Borrelia/genética
DNA Bacteriano/genética
Rickettsia/classificação
Rickettsia/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:28738085
[Au] Autor:Liu Y; Watson SC; Gettings JR; Lund RB; Nordone SK; Yabsley MJ; McMahan CS
[Ad] Endereço:Department of Mathematical Sciences, Clemson University, Clemson, SC, United States of America.
[Ti] Título:A Bayesian spatio-temporal model for forecasting Anaplasma species seroprevalence in domestic dogs within the contiguous United States.
[So] Source:PLoS One;12(7):e0182028, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This paper forecasts the 2016 canine Anaplasma spp. seroprevalence in the United States from eight climate, geographic and societal factors. The forecast's construction and an assessment of its performance are described. The forecast is based on a spatial-temporal conditional autoregressive model fitted to over 11 million Anaplasma spp. seroprevalence test results for dogs conducted in the 48 contiguous United States during 2011-2015. The forecast uses county-level data on eight predictive factors, including annual temperature, precipitation, relative humidity, county elevation, forestation coverage, surface water coverage, population density and median household income. Non-static factors are extrapolated into the forthcoming year with various statistical methods. The fitted model and factor extrapolations are used to estimate next year's regional prevalence. The correlation between the observed and model-estimated county-by-county Anaplasma spp. seroprevalence for the five-year period 2011-2015 is 0.902, demonstrating reasonable model accuracy. The weighted correlation (accounting for different sample sizes) between 2015 observed and forecasted county-by-county Anaplasma spp. seroprevalence is 0.987, exhibiting that the proposed approach can be used to accurately forecast Anaplasma spp. seroprevalence. The forecast presented herein can a priori alert veterinarians to areas expected to see Anaplasma spp. seroprevalence beyond the accepted endemic range. The proposed methods may prove useful for forecasting other diseases.
[Mh] Termos MeSH primário: Anaplasma/imunologia
Anaplasmose/sangue
Anaplasmose/imunologia
Anticorpos Antibacterianos/sangue
Doenças do Cão/sangue
Doenças do Cão/imunologia
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Clima
Cães
Previsões/métodos
Densidade Demográfica
Prevalência
Estudos Soroepidemiológicos
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182028


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[PMID]:28678004
[Au] Autor:Ochirkhuu N; Konnai S; Odbileg R; Murata S; Ohashi K
[Ad] Endereço:1 Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University , Sapporo, Japan .
[Ti] Título:Molecular Epidemiological Survey and Genetic Characterization of Anaplasma Species in Mongolian Livestock.
[So] Source:Vector Borne Zoonotic Dis;17(8):539-549, 2017 Aug.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. Few studies on Anaplasma infections in Mongolian livestock have been conducted. This study examined the prevalence of Anaplasma marginale, Anaplasma ovis, Anaplasma phagocytophilum, and Anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (Bos taurus), yaks (Bos grunniens), sheep (Ovis aries), and goats (Capra aegagrus hircus) in four provinces of Ulaanbaatar city in Mongolia. We genetically characterized positive samples through sequencing analysis based on the heat-shock protein groEL, major surface protein 4 (msp4), and 16S rRNA genes. Only A. ovis was detected in Mongolian livestock (cattle, yaks, sheep, and goats), with 413 animals (44.5%) positive for groEL and 308 animals (33.2%) positive for msp4 genes. In the phylogenetic tree, we separated A. ovis sequences into two distinct clusters based on the groEL gene. One cluster comprised sequences derived mainly from sheep and goats, which was similar to that in A. ovis isolates from other countries. The other divergent cluster comprised sequences derived from cattle and yaks and appeared to be newly branched from that in previously published single isolates in Mongolian cattle. In addition, the msp4 gene of A. ovis using same and different samples with groEL gene of the pathogen demonstrated that all sequences derived from all animal species, except for three sequences derived from cattle and yak, were clustered together, and were identical or similar to those in isolates from other countries. We used 16S rRNA gene sequences to investigate the genetically divergent A. ovis and identified high homology of 99.3-100%. However, the sequences derived from cattle did not match those derived from sheep and goats. The results of this study on the prevalence and molecular characterization of A. ovis in Mongolian livestock can facilitate the control of infectious diseases in livestock.
[Mh] Termos MeSH primário: Anaplasma/isolamento & purificação
Anaplasmose/microbiologia
Gado/microbiologia
Epidemiologia Molecular
[Mh] Termos MeSH secundário: Anaplasma/genética
Anaplasmose/epidemiologia
Animais
Chaperonina 60/genética
Chaperonina 60/metabolismo
DNA Bacteriano/genética
Regulação Bacteriana da Expressão Gênica
Mongólia/epidemiologia
Filogenia
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chaperonin 60); 0 (DNA, Bacterial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170706
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2017.2111


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[PMID]:28598270
[Au] Autor:Trout Fryxell RT; Hendricks BM; Pompo K; Mays SE; Paulsen DJ; Operario DJ; Houston AE
[Ad] Endereço:1 Department of Entomology and Plant Pathology, University of Tennessee , Knoxville, Tennessee.
[Ti] Título:Investigating the Adult Ixodid Tick Populations and Their Associated Anaplasma, Ehrlichia, and Rickettsia Bacteria at a Rocky Mountain Spotted Fever Hotspot in Western Tennessee.
[So] Source:Vector Borne Zoonotic Dis;17(8):527-538, 2017 Aug.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ehrlichiosis and rickettsiosis are two common bacterial tick-borne diseases in the southeastern United States. Ehrlichiosis is caused by ehrlichiae transmitted by Amblyomma americanum and rickettsiosis is caused by rickettsiae transmitted by Amblyomma maculatum and Dermacentor variabilis. These ticks are common and have overlapping distributions in the region. The objective of this study was to identify Anaplasma, Ehrlichia, and Rickettsia species associated with questing ticks in a Rocky Mountain spotted fever (RMSF) hotspot, and identify habitats, time periods, and collection methods for collecting questing-infected ticks. Using vegetation drags and CO -baited traps, ticks were collected six times (May-September 2012) from 100 sites (upland deciduous, bottomland deciduous, grassland, and coniferous habitats) in western Tennessee. Adult collections were screened for Anaplasma and Ehrlichia (simultaneous polymerase chain reaction [PCR]) and Rickettsia using genus-specific PCRs, and resulting positive amplicons were sequenced. Anaplasma and Ehrlichia were only identified within A. americanum (Ehrlichia ewingii, Ehrlichia chaffeensis, Panola Mountain Ehrlichia, and Anaplasma odocoilei sp. nov.); more Ehrlichia-infected A. americanum were collected at the end of June regardless of habitat and collection method. Rickettsia was identified in three tick species; "Candidatus Rickettsia amblyommii" from A. americanum, R. parkeri and R. andeanae from A. maculatum, and R. montanensis ( = montana) from D. variabilis. Overall, significantly more Rickettsia-infected ticks were identified as A. americanum and A. maculatum compared to D. variabilis; more infected-ticks were collected from sites May-July and with dragging. In this study, we report in the Tennessee RMSF hotspot the following: (1) Anaplasma and Ehrlichia are only found in A. americanum, (2) each tick species has its own Rickettsia species, (3) a majority of questing-infected ticks are collected May-July, (4) A. americanum and A. maculatum harbor pathogenic bacteria in western Tennessee, and (5) R. rickettsii remains unidentified.
[Mh] Termos MeSH primário: Anaplasma/isolamento & purificação
Ehrlichia/isolamento & purificação
Ixodidae/microbiologia
Rickettsia/isolamento & purificação
Febre Maculosa das Montanhas Rochosas/epidemiologia
[Mh] Termos MeSH secundário: Animais
Ecossistema
Seres Humanos
Febre Maculosa das Montanhas Rochosas/microbiologia
Estações do Ano
Tennessee/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2016.2091


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[PMID]:28438088
[Au] Autor:Pennisi MG; Hofmann-Lehmann R; Radford AD; Tasker S; Belák S; Addie DD; Boucraut-Baralon C; Egberink H; Frymus T; Gruffydd-Jones T; Hartmann K; Horzinek MC; Hosie MJ; Lloret A; Lutz H; Marsilio F; Thiry E; Truyen U; Möstl K
[Ti] Título:Anaplasma, Ehrlichia and Rickettsia species infections in cats: European guidelines from the ABCD on prevention and management
[So] Source:J Feline Med Surg;19(5):542-548, 2017 05.
[Is] ISSN:1532-2750
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OVERVIEW: Anaplasma species, Ehrlichia species and Rickettsia species are vector-borne pathogens infecting a wide variety of mammals, but causing disease in very few of them. Infection in cats: Anaplasma phagocytophilum is the most important feline pathogen among these rickettsial organisms, and coinfections are possible. Little information is available on the pathogenesis of these agents in cats. Clinical signs are usually reported soon after tick infestation. They are mostly non-specific, consisting of fever, anorexia and lethargy. Joint pain may occur. Infection in humans: Some rickettsial species ( A phagocytophilum, Ehrlichia chaffeensis, Ehrlichia ewingii, Rickettsia conorii, Rickettsia rickettsii, Rickettsia felis, Rickettsia typhi and Candidatus Neoehrlichia mikurensis) are of zoonotic concern. Direct contact with cat saliva should be avoided because of potential contamination by R felis. Infected cats are 'sentinels' of the presence of rickettsial pathogens in ticks and fleas in a given geographical area, and they signal a risk for people exposed to vectors.
[Mh] Termos MeSH primário: Anaplasmose
Doenças do Gato
Ehrlichiose/veterinária
Infecções por Rickettsia/veterinária
[Mh] Termos MeSH secundário: Anaplasma/fisiologia
Anaplasmose/diagnóstico
Anaplasmose/tratamento farmacológico
Anaplasmose/microbiologia
Anaplasmose/prevenção & controle
Animais
Doenças do Gato/diagnóstico
Doenças do Gato/tratamento farmacológico
Doenças do Gato/microbiologia
Doenças do Gato/prevenção & controle
Gatos
Ehrlichia/fisiologia
Ehrlichiose/diagnóstico
Ehrlichiose/microbiologia
Ehrlichiose/terapia
Seres Humanos
Rickettsia/fisiologia
Infecções por Rickettsia/diagnóstico
Infecções por Rickettsia/microbiologia
Infecções por Rickettsia/terapia
[Pt] Tipo de publicação:GUIDELINE; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1177/1098612X17706462


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[PMID]:28410950
[Au] Autor:Elhamiani Khatat S; Khallaayoune K; Errafyk N; Van Gool F; Duchateau L; Daminet S; Kachani M; El Amri H; Azrib R; Sahibi H
[Ad] Endereço:Institut Agronomique et Vétérinaire Hassan II, Madinat Al Irfane, BP 6202, Rabat-Instituts, 10101, Morocco; Faculty of Veterinary Medicine of Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium. Electronic address: elhamianis@yahoo.fr.
[Ti] Título:Detection of Anaplasma spp. and Ehrlichia spp. anibodies, and Dirofilaria immitis antigens in dogs from seven locations of Morocco.
[So] Source:Vet Parasitol;239:86-89, 2017 May 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In Morocco no data has been published on canine exposure to Anaplasma spp., Borrrelia burgdorferi, and Ehrlichia spp., and only one report is available on the occurrence of Dirofilaria immitis in dogs. Therefore, the aim of this study was to collect current data on the canine exposure to these vector-borne pathogens (VBPs) in Morocco. A total of 217 urban (n=57), rural (n=110) and military (n=50) dogs from seven Moroccan locations were screened for Anaplasma spp., B. burgdorferi and Ehrlichia spp. antibodies and for D. immitis antigens using a commercial in-clinic ELISA test. Of these dogs, 182 (83.9%) tested positive for at least one pathogen and positivity to two or three pathogens was found in 14.3% and 2.3% of the dogs, respectively. Ehrlichia spp. antibodies (34.6%) were the most frequently detected followed by Anaplasma spp. antibodies (16.6%) and D. immitis antigens (16.1%). None of the dogs was tested seropositive to B. burgdorferi. Statistically significant differences in seropositivity rates were found for Ehrlichia spp. and D. immitis in rural dogs especially those from the north central region (p<0.001) but not for Anaplasma spp. No significant difference was found according to the health status of the dog. This study demonstrates that Moroccan dogs are at high risk of acquiring a vector-borne infection.
[Mh] Termos MeSH primário: Anaplasma/imunologia
Anaplasmose/imunologia
Dirofilaria immitis/imunologia
Dirofilariose/imunologia
Doenças do Cão/parasitologia
Ehrlichia/imunologia
[Mh] Termos MeSH secundário: Anaplasmose/epidemiologia
Animais
Anticorpos Antibacterianos/sangue
Antígenos de Helmintos/sangue
Dirofilariose/epidemiologia
Doenças do Cão/epidemiologia
Cães
Ehrlichiose/epidemiologia
Ehrlichiose/imunologia
Ehrlichiose/veterinária
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Helminth)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE


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[PMID]:28288773
[Au] Autor:Montenegro VM; Bonilla MC; Kaminsky D; Romero-Zúñiga JJ; Siebert S; Krämer F
[Ad] Endereço:Laboratorio de Parasitología, Escuela de Medicina Veterinaria, Universidad Nacional, Campus Benjamín Núñez, Heredia, Costa Rica.
[Ti] Título:Serological detection of antibodies to Anaplasma spp., Borrelia burgdorferi sensu lato and Ehrlichia canis and of Dirofilaria immitis antigen in dogs from Costa Rica.
[So] Source:Vet Parasitol;236:97-107, 2017 Mar 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In a study in Costa Rica 314 serum samples from dogs throughout all seven provinces were tested using a commercial kit for the detection of circulating antibodies against Anaplasma spp., Borrelia burgdorferi sensu lato and Ehrlichia canis, and of circulating antigen of Dirofilaria immitis. A total of 6.4% (20/314) and 38.2% (120/314) were positive for Anaplasma spp. (An) and E. canis (Ec) antibodies. Overall, 8.0% (25/314) were positive for D. immitis (Di) antigen. One single dog reacted positive with B. burgdorferi s.l. (Bb) antigen (0.3%, 1/314). E. canis positive dogs were detected in all provinces (highest percentages in Guanacaste, Puntarenas [both significantly different compared to the overall] and Limón). Guanacaste and Puntarenas also showed the highest prevalences of Anaplasma spp. (both significantly different compared to the overall). The highest prevalence of D. immitis was detected in Puntarenas (significantly different compared to the overall). Double pathogen exposure (Ec plus An; Ec plus Di; Ec plus Bb) were recorded in 8.9% (28/314). Two dogs showed a triple pathogen exposure (0.6%, 2/314; An, Ec and Di). There was a significant difference between male (11.5%, 18/156) and female (4.4%, 7/158) animals for D. immitis positive results. There was also a significant difference between breed and no breed dogs regarding the characteristics of a general positive test, as well as seropositivity to the single pathogens of Anaplasma spp., E. canis and D. immitis. Finally there was a significant difference in the presence of clinical signs again regarding the characteristics of a general positive test, as well as seropositivity to Anaplasma spp., E. canis and D. immitis. Practitioners in Costa Rica should be aware of the canine vector-borne diseases mentioned as dogs are at risk of becoming infected. Concerning the positive B. burgdorferi s.l. dog, an autochthonous occurrence cannot be confirmed due to a history of adoption and an unusual tattoo number. Veterinary advice to protect dogs and limit transmission of vector-borne pathogens, also to humans, by using prophylactic measures is strongly recommended.
[Mh] Termos MeSH primário: Anaplasmose/epidemiologia
Dirofilariose/epidemiologia
Doenças do Cão/epidemiologia
Ehrlichiose/veterinária
Doença de Lyme/veterinária
[Mh] Termos MeSH secundário: Fatores Etários
Anaplasma/isolamento & purificação
Anaplasmose/genética
Anaplasmose/microbiologia
Animais
Anticorpos Antibacterianos/sangue
Antígenos de Helmintos/sangue
Grupo Borrelia Burgdorferi/isolamento & purificação
Costa Rica/epidemiologia
Dirofilaria immitis/isolamento & purificação
Dirofilariose/genética
Dirofilariose/parasitologia
Doenças do Cão/genética
Doenças do Cão/microbiologia
Doenças do Cão/parasitologia
Cães
Ehrlichia canis/isolamento & purificação
Ehrlichiose/epidemiologia
Ehrlichiose/genética
Ehrlichiose/microbiologia
Feminino
Doença de Lyme/epidemiologia
Doença de Lyme/genética
Doença de Lyme/microbiologia
Masculino
Prevalência
Fatores de Risco
Estudos Soroepidemiológicos
Fatores Sexuais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Helminth)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE


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[PMID]:28285597
[Au] Autor:Attipa C; Papasouliotis K; Solano-Gallego L; Baneth G; Nachum-Biala Y; Sarvani E; Knowles TG; Mengi S; Morris D; Helps C; Tasker S
[Ad] Endereço:Molecular Diagnostic Unit, Diagnostic Laboratories, Langford Vets and School of Veterinary Sciences, University of Bristol, Langford, UK. attipacy@gmail.com.
[Ti] Título:Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus.
[So] Source:Parasit Vectors;10(1):130, 2017 Mar 13.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Feline infectious agent studies are lacking in Cyprus. The aims of this study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (FVBP), in cats from Cyprus. METHODS: A cross-sectional, descriptive, multicentre study was performed on 174 feline samples [138 owned and 36 shelter-feral, including both healthy (43) and non-healthy (131), cats] from private veterinary clinics from all six districts of Cyprus. Real-time quantitative polymerase chain reaction (qPCR) assays were used to detect Mycoplasma haemofelis (Mhf), "Candidatus Mycoplasma haemominutum" (CMhm) and "Candidatus Mycoplasma turicensis" (CMt). The population was tested for four FVBP including Bartonella henselae and Leishmania spp. using qPCR, while conventional PCR assays were used to detect Ehrlichia/Anaplasma spp. and Hepatozoon spp. Serological assays were performed to detect Leishmania infantum antibodies, feline leukaemia virus (FeLV) antigen and feline immunodeficiency virus (FIV) antibodies. Statistical analysis was performed to test associations and possible risk factors between variables and infectious agents. RESULTS: Ninety-six (55.2%) of the 174 cats were PCR-positive for at least one infectious agent. Forty-six cats (26.4%) were haemoplasma positive, including 13 (7.5%) for Mhf, 36 (20.7%) for CMhm and 12 (6.9%) for CMt. Sixty-six cats (37.9%) were positive for Hepatozoon spp., while 19 (10.9%) were positive for B. henselae, four (2.3%) for Leishmania spp. and one (0.6%) for Ehrlichia/Anaplasma spp. Sequencing revealed the presence of Hepatozoon felis, L. infantum and Anaplasma platys. Of the 164 cats that underwent retroviral serology, 10 (6.1%) were FeLV-positive and 31 (18.9%) were FIV-positive, while L. infantum serology was positive in 7 (4.4%) of the 160 cats tested. Multivariable logistic regression revealed significant associations for various infectious agents including L. infantum with each of Hepatozoon spp. and CMt infection. CONCLUSIONS: A high prevalence of infectious agents was found in cats from Cyprus with Mhf, CMhm, CMt, L. infantum, B. henselae, H. felis, A. platys, FeLV and FIV infections reported for the first time. The significant associations between different pathogens provide a better understanding of similarities in the epidemiology of these pathogens and interactions between them.
[Mh] Termos MeSH primário: Infecções Bacterianas/veterinária
Doenças do Gato/epidemiologia
Infecções por Mycoplasma/veterinária
Infecções Protozoárias em Animais/epidemiologia
Infecções por Retroviridae/veterinária
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Anaplasma/genética
Anaplasma/isolamento & purificação
Anaplasmose/epidemiologia
Anaplasmose/microbiologia
Animais
Infecções Bacterianas/epidemiologia
Infecções Bacterianas/microbiologia
Doenças do Gato/microbiologia
Doenças do Gato/parasitologia
Doenças do Gato/virologia
Gatos
Coccidiose/epidemiologia
Coccidiose/veterinária
Estudos Transversais
Chipre/epidemiologia
Ehrlichia/genética
Ehrlichia/isolamento & purificação
Ehrlichiose/epidemiologia
Ehrlichiose/microbiologia
Ehrlichiose/veterinária
Análise Fatorial
Feminino
Leishmania infantum/genética
Leishmania infantum/isolamento & purificação
Leishmaniose Visceral/epidemiologia
Leishmaniose Visceral/parasitologia
Leishmaniose Visceral/veterinária
Vírus da Leucemia Felina/genética
Vírus da Leucemia Felina/isolamento & purificação
Masculino
Mycoplasma/genética
Mycoplasma/isolamento & purificação
Infecções por Mycoplasma/epidemiologia
Infecções por Mycoplasma/microbiologia
Prevalência
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Análise de Regressão
Infecções por Retroviridae/epidemiologia
Infecções por Retroviridae/virologia
Medição de Risco
Fatores de Risco
Doenças Transmitidas por Carrapatos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2063-2


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[PMID]:28285585
[Au] Autor:Irwin PJ; Robertson ID; Westman ME; Perkins M; Straubinger RK
[Ad] Endereço:Vector and Water-Borne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia. P.Irwin@murdoch.edu.au.
[Ti] Título:Searching for Lyme borreliosis in Australia: results of a canine sentinel study.
[So] Source:Parasit Vectors;10(1):114, 2017 Mar 13.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Lyme borreliosis is a common tick-borne disease of the northern hemisphere that is caused by bacterial spirochaetes of the Borrelia burgdorferi (sensu lato) (Bbsl) complex. To date, there has been no convincing evidence for locally-acquired Lyme borreliosis on the Australian continent and there is currently a national debate concerning the nature and distributions of zoonotic tick-transmitted infectious disease in Australia. In studies conducted in Europe and the United States, dogs have been used as sentinels for tick-associated illness in people since they readily contact ticks that may harbour zoonotic pathogens. Applying this principle, we used a combination of serological assays to test dogs living in tick 'hot spots' and exposed to the Australian paralysis tick, Ixodes holocyclus, for evidence of exposure to B. burgdorferi (s.l.) antigens and other vector-borne pathogens. RESULTS: Altogether, 555 dogs from four demographic groups were recruited into this study. One dog had evidence of exposure to Anaplasma spp. but no other dog was positive in screening tests. A total of 122 dogs (22.0%) had a kinetic ELISA (KELA) unit value > 100, and one dog with a high titre (399.9 KELA units) had been vaccinated against B. burgdorferi (sensu stricto) before travelling to Australia. Older dogs and those with a history of tick paralysis were significantly more likely to have a KELA unit value > 100. Line immunoassay analysis revealed moderate-to-weak (equivocal) bands in 27 (4.9%) dogs. CONCLUSIONS: Except for a single dog presumed to have been exposed to Anaplasma platys, infection with Anaplasma spp. B. burgdorferi (s.l.), Ehrlichia spp., and Dirofilaria immitis, was not detected in the cohort of Australian dogs evaluated in this study. These results provide further evidence that Lyme borreliosis does not exist in Australia but that cross-reacting antibodies (false positive results) are common and may be caused by the transmission of other tick-associated organisms.
[Mh] Termos MeSH primário: Doenças do Cão/epidemiologia
Doença de Lyme/veterinária
Espécies Sentinelas/microbiologia
Espécies Sentinelas/parasitologia
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Anaplasma/imunologia
Animais
Anticorpos Antibacterianos/sangue
Austrália/epidemiologia
Borrelia burgdorferi/imunologia
Borrelia burgdorferi/isolamento & purificação
Doenças do Cão/diagnóstico
Doenças do Cão/imunologia
Doenças do Cão/microbiologia
Cães
Ehrlichia/imunologia
Ehrlichiose/veterinária
Ensaio de Imunoadsorção Enzimática
Ixodes/microbiologia
Doença de Lyme/diagnóstico
Doença de Lyme/epidemiologia
Doença de Lyme/imunologia
Espécies Sentinelas/imunologia
Testes Sorológicos
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/imunologia
Doenças Transmitidas por Carrapatos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2058-z


  10 / 891 MEDLINE  
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[PMID]:28285583
[Au] Autor:Movilla R; Altet L; Serrano L; Tabar MD; Roura X
[Ad] Endereço:Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Carrer de L'Hospital s/n, 08193 Bellaterra, Cerdanyola del Vallès, Barcelona, Spain.
[Ti] Título:Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease.
[So] Source:Parasit Vectors;10(1):131, 2017 Mar 13.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (VBP), which could have a fundamental role in the pathogenesis of splenic disease. This study aimed to evaluate certain VBP in samples from dogs with splenic lesions. METHODS: Seventy-seven EDTA-blood and 64 splenic tissue samples were collected from 78 dogs with splenic disease in a Mediterranean area. Babesia spp., Bartonella spp., Ehrlichia/Anaplasma spp., Hepatozoon canis, Leishmania infantum, hemotropic Mycoplasma spp. and Rickettsia spp. were targeted using PCR assays. Sixty EDTA-blood samples from dogs without evidence of splenic lesions were included as a control group. RESULTS: More than half (51.56%) of the biopsies (33/64) were consistent with benign lesions and 48.43% (31/64) with malignancy, mostly hemangiosarcoma (25/31). PCR yielded positive results in 13 dogs with spleen alterations (16.67%), for Babesia canis (n = 3), Babesia gibsoni (n = 2), hemotropic Mycoplasma spp. (n = 2), Rickettsia massiliae (n = 1) and "Babesia vulpes" (n = 1), in blood; and for B. canis, B. gibsoni, Ehrlichia canis and L. infantum (n = 1 each), in spleen. Two control dogs (3.3%) were positive for B. gibsoni and H. canis (n = 1 each). Benign lesions were detected in the 61.54% of infected dogs (8/13); the remaining 38.46% were diagnosed with malignancies (5/13). Infection was significantly associated to the presence of splenic disease (P = 0.013). There was no difference in the prevalence of infection between dogs with benign and malignant splenic lesions (P = 0.69); however B. canis was more prevalent in dogs with hemangiosarcoma (P = 0.006). CONCLUSIONS: VBP infection could be involved in the pathogenesis of splenic disease. The immunological role of the spleen could predispose to alterations of this organ in infected dogs. Interestingly, all dogs with B. canis infection were diagnosed with hemangiosarcoma in the present survey. As previously reported, results support that VBP diagnosis could be improved by analysis of samples from different tissues. The sample size included here warrants further investigation.
[Mh] Termos MeSH primário: Doenças do Cão/microbiologia
Doenças do Cão/parasitologia
Baço/microbiologia
Baço/parasitologia
Esplenopatias/veterinária
[Mh] Termos MeSH secundário: Anaplasma/genética
Anaplasmose/sangue
Anaplasmose/diagnóstico
Animais
Babesia/genética
Babesia/isolamento & purificação
Babesiose/sangue
Babesiose/diagnóstico
Bartonella/genética
Bartonella/isolamento & purificação
Infecções por Bartonella/diagnóstico
Infecções por Bartonella/veterinária
Vetores de Doenças
Doenças do Cão/sangue
Doenças do Cão/diagnóstico
Cães
Ehrlichia canis/genética
Ehrlichiose/diagnóstico
Ehrlichiose/veterinária
Leishmania infantum/genética
Leishmania infantum/isolamento & purificação
Leishmaniose Visceral/diagnóstico
Leishmaniose Visceral/veterinária
Mycoplasma/genética
Mycoplasma/isolamento & purificação
Infecções por Mycoplasma/diagnóstico
Infecções por Mycoplasma/veterinária
Reação em Cadeia da Polimerase/métodos
Rickettsia/genética
Rickettsia/isolamento & purificação
Infecções por Rickettsia/diagnóstico
Infecções por Rickettsia/veterinária
Baço/patologia
Esplenopatias/diagnóstico
Esplenopatias/microbiologia
Esplenopatias/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2074-z



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