Base de dados : MEDLINE
Pesquisa : B03.440.040.050.100 [Categoria DeCS]
Referências encontradas : 19 [refinar]
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  1 / 19 MEDLINE  
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[PMID]:28155283
[Au] Autor:Chaisi ME; Baxter JR; Hove P; Choopa CN; Oosthuizen MC; Brayton KA; Khumalo ZT; Mutshembele AM; Mtshali MS; Collins NE
[Ad] Endereço:Department of Veterinary Tropical Diseases, University of Pretoria. mechaisi@yahoo.co.uk.
[Ti] Título:Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle.
[So] Source:Onderstepoort J Vet Res;84(1):e1-e9, 2017 Jan 23.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Several nucleic acid-based assays have been developed for detecting Anaplasma marginale and Anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas difficult. We evaluated the ability of the reverse line blot (RLB) hybridisation assay, two nested polymerase chain reaction (nPCR) assays and a duplex real-time quantitative polymerase chain reaction (qPCR) assay to detect A. marginale and A. centrale infections in cattle (n = 66) in South Africa. The lowest detection limits for A. marginale plasmid DNA were 2500 copies by the RLB assay, 250 copies by the nPCR and qPCR assays and 2500, 250 and 25 copies of A. centrale plasmid DNA by the RLB, nPCR and qPCR assays respectively. The qPCR assay detected more A. marginale- and A. centrale-positive samples than the other assays, either as single or mixed infections. Although the results of the qPCR and nPCR tests were in agreement for the majority (38) of A. marginale-positive samples, 13 samples tested negative for A. marginale using nPCR but positive using qPCR. To explain this discrepancy, the target sequence region of the nPCR assay was evaluated by cloning and sequencing the msp1ß gene from selected field samples. The results indicated sequence variation in the internal forward primer (AM100) area amongst the South African A. marginale msp1ß sequences, resulting in false negatives. We propose the use of the duplex qPCR assay in future studies as it is more sensitive and offers the benefits of quantification and multiplex detection of both Anaplasma spp.
[Mh] Termos MeSH primário: Anaplasma centrale
Anaplasma marginale
Anaplasmose/diagnóstico
Doenças dos Bovinos/diagnóstico
Hibridização de Ácido Nucleico
Reação em Cadeia da Polimerase/veterinária
Reação em Cadeia da Polimerase em Tempo Real/veterinária
[Mh] Termos MeSH secundário: Anaplasma centrale/genética
Anaplasma marginale/genética
Anaplasmose/microbiologia
Animais
Bovinos
Doenças dos Bovinos/microbiologia
DNA Bacteriano/genética
Hibridização de Ácido Nucleico/métodos
Reação em Cadeia da Polimerase/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v84i1.1262


  2 / 19 MEDLINE  
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[PMID]:26809916
[Au] Autor:Hosgör M; Bilgiç HB; Bakirci S; Ünlü AH; Karagenç T; Eren H
[Ad] Endereço:Gida, Tarim ve Hayvancilik Bakanligi, Karpuzlu Ilçe Gida Tarim ve Hayvancilik Müdürlügü, Aydin, Türkiye. hbilgic@adu.edu.tr.
[Ti] Título:[Detection of Anaplasma / Ehrlichia Species of Cattle and Ticks in Aydin Region].
[Ti] Título:Aydin Yöresinde Sigirlarda ve Kenelerde Anaplasma / Ehrlichia Türlerinin Belirlenmesi..
[So] Source:Turkiye Parazitol Derg;39(4):291-8, 2015 Dec.
[Is] ISSN:2146-3077
[Cp] País de publicação:Turkey
[La] Idioma:tur
[Ab] Resumo:OBJECTIVE: The aim of this study is to detect the Anaplasma/Ehrlichia species of cattle and ticks and to provide knowledge on the prevalence of these species during sampling periods. METHODS: A total of 679 blood and 186 tick samples were collected from the Osmanbükü, Akçaova, Dalama, and Söke districts of Aydin. The samples were screened with genus polymerase chain reaction (PCR) for Anaplasma/Ehrlichia spp., species-specific polymerase chain reaction for Anaplasma marginale and A. centrale, and nested PCR for A. bovis and A. phagocytophilum. RESULTS: A. centrale was detected in Söke during September and in Dalama and Akçaova during March, June, September, and December. A. marginale was detected in Osmanbükü during June; in Söke during March and December; in Akçaova during June, September, and March; and in Dalama during the entire sampling period. A. phagocytophilum was detected in all regions during the entire sampling period. None of the samples were positive for A. bovis. Mixed infections were detected in 50 blood samples. A. marginale and A. phagocytophilum were detected in the tick samples. CONCLUSION: In this study, A. phagocytophilum was abundantly detected compared with A. marginale and A. centrale. A. phagocytophilum and A. centrale were extensively found in Akçaova and A. marginale was mostly seen in Dalama. Parasites were extensively detected in September and March. The analysis indicated that collected ticks were infected with different Anaplasma/Ehrlichia species.
[Mh] Termos MeSH primário: Anaplasma centrale/isolamento & purificação
Anaplasma marginale/isolamento & purificação
Anaplasma phagocytophilum/isolamento & purificação
Anaplasmose/parasitologia
Doenças dos Bovinos/parasitologia
Ehrlichiose/veterinária
[Mh] Termos MeSH secundário: Anaplasma centrale/genética
Anaplasma marginale/genética
Anaplasma phagocytophilum/genética
Anaplasmose/epidemiologia
Anaplasmose/transmissão
Animais
Vetores Aracnídeos/parasitologia
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/transmissão
Ehrlichiose/epidemiologia
Ehrlichiose/parasitologia
Ehrlichiose/transmissão
Reação em Cadeia da Polimerase/veterinária
Especificidade da Espécie
Carrapatos/parasitologia
Turquia/epidemiologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160127
[St] Status:MEDLINE
[do] DOI:10.5152/tpd.2015.4525


  3 / 19 MEDLINE  
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[PMID]:26210950
[Au] Autor:Bell-Sakyi L; Palomar AM; Bradford EL; Shkap V
[Ad] Endereço:The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK. Electronic address: lesley.sakyi@pirbright.ac.uk.
[Ti] Título:Propagation of the Israeli vaccine strain of Anaplasma centrale in tick cell lines.
[So] Source:Vet Microbiol;179(3-4):270-6, 2015 Sep 30.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Anaplasma centrale has been used in cattle as a live blood vaccine against the more pathogenic Anaplasma marginale for over 100 years. While A. marginale can be propagated in vitro in tick cell lines, facilitating studies on antigen production, immunisation and vector-pathogen interaction, to date there has been no in vitro culture system for A. centrale. In the present study, 25 cell lines derived from 13 ixodid tick species were inoculated with the Israeli vaccine strain of A. centrale and monitored for at least 12 weeks by microscopic examination of Giemsa-stained cytocentrifuge smears. Infection of 19 tick cell lines was subsequently attempted by transfer of cell-free supernate from vaccine-inoculated tick cells. In two separate experiments, rickettsial inclusions were detected in cultures of the Rhipicephalus appendiculatus cell line RAE25 28-32 days following inoculation with the vaccine. Presence of A. centrale in the RAE25 cells was confirmed by PCR assays targeting the 16S rRNA, groEL and msp4 genes; sequenced PCR products were 100% identical to published sequences of the respective genes in the Israeli vaccine strain of A. centrale. A. centrale was taken through three subcultures in RAE25 cells over a 30 week period. In a single experiment, the Dermacentor variabilis cell line DVE1 was also detectably infected with A. centrale 11 weeks after inoculation with the vaccine. Availability of an in vitro culture system for A. centrale in tick cells opens up the possibility of generating a safer and more ethical vaccine for bovine anaplasmosis.
[Mh] Termos MeSH primário: Anaplasma centrale/crescimento & desenvolvimento
Anaplasmose/prevenção & controle
Vacinas Bacterianas/imunologia
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/prevenção & controle
Ixodidae/citologia
Ixodidae/microbiologia
[Mh] Termos MeSH secundário: Anaplasma centrale/genética
Anaplasma centrale/imunologia
Anaplasmose/imunologia
Animais
Sequência de Bases
Bovinos
Doenças dos Bovinos/imunologia
Técnicas de Cultura de Células/métodos
Linhagem Celular
Chaperonina 60/genética
Dados de Sequência Molecular
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vacinação/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Vaccines); 0 (Chaperonin 60); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150930
[Lr] Data última revisão:
150930
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150727
[St] Status:MEDLINE


  4 / 19 MEDLINE  
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[PMID]:26117444
[Au] Autor:Belkahia H; Ben Said M; Alberti A; Abdi K; Issaoui Z; Hattab D; Gharbi M; Messadi L
[Ad] Endereço:Laboratoire de Microbiologie, Ecole Nationale de Médecine Vétérinaire, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, Université de La Manouba, 2020 Sidi Thabet, Tunisia; Faculté des Sciences de Bizerte, Université de Carthage, 7021 Jarzouna, Tunisia.
[Ti] Título:First molecular survey and novel genetic variants' identification of Anaplasma marginale, A. centrale and A. bovis in cattle from Tunisia.
[So] Source:Infect Genet Evol;34:361-71, 2015 Aug.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Few data are available about the presence and distribution of Anaplasma species in cattle in North African countries. In this study prevalence, co-infections, risk factors and genetic diversity of Anaplasma species were evaluated in bovines from Northern Tunisia. A total of 232 cattle from 36 randomly selected farms in three Tunisian localities were investigated for the presence of Anaplasma species in blood by Real-time PCR and/or nested PCR. Overall infection rates of Anaplasma spp., Anaplasma marginale, Anaplasma centrale and Anaplasma bovis were 34.9%, 25.4%, 15.1%, and 3.9%, respectively. Anaplasma phagocytophilum was not detected in cattle. The most common co-infection pattern was an association of A. marginale and A. centrale (11.2%). Five cattle (2.1%) all reared in the sub-humid bioclimatic area, were co-infected by the three Anaplasma species. Molecular prevalence of Anaplasma infection varied significantly according to locality, bioclimatic area, tick infestation and type of breeding. Animals of the Holstein breed were less infected by A. marginale and A. centrale than other breeds. Genetic analysis of A. marginale msp4 gene indicated a high sequence diversity of Tunisian strains, suggesting a multiple introduction of infected cattle from different origins. Phylogenetic studies based on the 16S rRNA gene showed that the most prevalent A. centrale strains were closely related to the A. centrale vaccine strain. Moreover, all A. bovis variants clustered with other A. bovis sequences obtained from domestic and wild ruminant strains. This is the first molecular investigation on Anaplasma species in Tunisian cattle providing pivotal background for designing epidemiological studies and to develop control strategies in the country.
[Mh] Termos MeSH primário: Anaplasma centrale/genética
Anaplasma marginale/genética
Anaplasmose/microbiologia
Doenças dos Bovinos/microbiologia
[Mh] Termos MeSH secundário: Anaplasmose/epidemiologia
Animais
Proteínas de Bactérias/genética
Bovinos
Doenças dos Bovinos/epidemiologia
Variação Genética
Proteínas de Membrana/genética
Tipagem Molecular
Filogenia
RNA Ribossômico 16S/genética
Tunísia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Membrane Proteins); 0 (Msp4 protein, Anaplasma marginale); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150808
[Lr] Data última revisão:
150808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150629
[St] Status:MEDLINE


  5 / 19 MEDLINE  
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[PMID]:26111122
[Au] Autor:Palomar AM; Portillo A; Santibáñez P; Mazuelas D; Roncero L; García-Álvarez L; Santibáñez S; Gutiérrez Ó; Oteo JA
[Ad] Endereço:Centre of Rickettsiosis and Arthropod-Borne Diseases, Hospital San Pedro-CIBIR, Logroño, La Rioja, Spain.
[Ti] Título:Detection of tick-borne Anaplasma bovis, Anaplasma phagocytophilum and Anaplasma centrale in Spain.
[So] Source:Med Vet Entomol;29(3):349-53, 2015 Sep.
[Is] ISSN:1365-2915
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The genus Anaplasma (Rickettsiales: Anaplasmataceae) includes species of medical and veterinary importance. The presence of Anaplasma spp. in ticks from birds, as well as in Haemaphysalis punctata (Ixodida: Ixodidae) specimens collected from cattle and vegetation in northern Spain was investigated. A total of 336 ticks from birds [174 Ixodes frontalis (Ixodida: Ixodidae), 108 H. punctata, 34 Hyalomma marginatum (Ixodida: Ixodidae), 17 Ixodes ricinus (Ixodida: Ixodidae) and three Ixodes spp.], and 181 H. punctata specimens collected from cattle (n = 71) and vegetation (n = 110) were analysed. Anaplasma bovis was detected in five H. punctata, including two from birds (1.9%) and three from vegetation (2.7%). Four I. frontalis (2.3%) (one co-infected with 'Candidatus Midichloria mitochondrii') and one I. ricinus (5.9%) removed from birds, as well as four H. punctata (5.6%) collected from cattle showed Anaplasma phagocytophilum infection. In addition, Anaplasma centrale was found in two H. punctata, one from a cow (1.4%) and the other from vegetation (0.9%). This study represents the first evidence of the presence of A. bovis in European ticks, and reports the first detection of A. bovis and A. centrale in H. punctata, and the first finding of A. phagocytophilum and 'Ca. Midichloria mitochondrii' in I. frontalis.
[Mh] Termos MeSH primário: Anaplasma/fisiologia
Doenças das Aves/epidemiologia
Doenças dos Bovinos/epidemiologia
Ixodidae/microbiologia
Doenças Transmitidas por Carrapatos/epidemiologia
[Mh] Termos MeSH secundário: Anaplasma centrale/fisiologia
Anaplasma phagocytophilum/fisiologia
Anaplasmose/epidemiologia
Anaplasmose/microbiologia
Animais
Doenças das Aves/microbiologia
Bovinos
Doenças dos Bovinos/microbiologia
Ehrlichiose/epidemiologia
Ehrlichiose/microbiologia
Feminino
Ixodes/crescimento & desenvolvimento
Ixodes/microbiologia
Ixodidae/crescimento & desenvolvimento
Larva/crescimento & desenvolvimento
Larva/microbiologia
Masculino
Ninfa/crescimento & desenvolvimento
Ninfa/microbiologia
Espanha/epidemiologia
Doenças Transmitidas por Carrapatos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150725
[Lr] Data última revisão:
150725
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150626
[St] Status:MEDLINE
[do] DOI:10.1111/mve.12124


  6 / 19 MEDLINE  
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[PMID]:21189322
[Au] Autor:Agnes JT; Brayton KA; LaFollett M; Norimine J; Brown WC; Palmer GH
[Ad] Endereço:Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA.
[Ti] Título:Identification of Anaplasma marginale outer membrane protein antigens conserved between A. marginale sensu stricto strains and the live A. marginale subsp. centrale vaccine.
[So] Source:Infect Immun;79(3):1311-8, 2011 Mar.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Live vaccination with Anaplasma marginale subsp. centrale (synonym for Anaplasma centrale) induces protection against severe disease upon challenge with A. marginale sensu stricto strains. Despite over a century of field use, the targets of protective immunity remained unknown. Using a broad proteomic approach, we identified the proteins in a challenge sensu stricto strain that were bound by the relevant antibody isotype induced by live vaccination with Anaplasma marginale subsp. centrale. A core of 15 proteins was identified in vaccinated animals across multiple major histocompatibility complex (MHC) haplotypes. This core separated into two structural/functional classes: "housekeeping" proteins involved in replication and metabolism and outer membrane proteins (OMPs). Orthologous proteins of both classes were identified within the vaccine strain and among sensu stricto strains. In contrast to the broad conservation among strains in the sequences of the housekeeping proteins, there was significantly greater divergence in the OMPs and greater divergence in both OMP sequences and the encoding locus structure between the vaccine strain and the sensu stricto strains than among the sensu stricto strains. The OMPs bound by live vaccine-induced antibody overlapped with OMPs that were immunogenic in animals vaccinated with inactivated vaccines and subsequently protected against bacteremia and disease. The identification of this core set of OMPs is consistent with the hypothesis that "subdominant" immunogens are required for vaccine-induced protection against A. marginale and provides clear direction for development of a safer, more effective vaccine.
[Mh] Termos MeSH primário: Anaplasma centrale/genética
Anaplasma marginale/genética
Proteínas da Membrana Bacteriana Externa/genética
Vacinas Bacterianas/imunologia
[Mh] Termos MeSH secundário: Anaplasma centrale/imunologia
Anaplasma marginale/imunologia
Anaplasmose/genética
Anaplasmose/imunologia
Anaplasmose/prevenção & controle
Animais
Antígenos de Bactérias/genética
Antígenos de Bactérias/imunologia
Proteínas da Membrana Bacteriana Externa/imunologia
Vacinas Bacterianas/genética
Sequência de Bases
Bovinos
Cromatografia Líquida
Sequência Conservada
Eletroforese em Gel Bidimensional
Immunoblotting
Dados de Sequência Molecular
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Bacterial Outer Membrane Proteins); 0 (Bacterial Vaccines)
[Em] Mês de entrada:1104
[Cu] Atualização por classe:161028
[Lr] Data última revisão:
161028
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101230
[St] Status:MEDLINE
[do] DOI:10.1128/IAI.01174-10


  7 / 19 MEDLINE  
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[PMID]:20528172
[Au] Autor:Portillo A; Pérez-Martínez L; Santibáñez S; Santibáñez P; Palomar AM; Oteo JA
[Ad] Endereço:Área de Enfermedades Infecciosas (Centro de Rickettsiosis y Enfermedades Transmitidas por Artrópodos Vectores), Hospital San Pedro-CIBIR, Logroño, Spain.
[Ti] Título:Anaplasma spp. in wild mammals and Ixodes ricinus from the north of Spain.
[So] Source:Vector Borne Zoonotic Dis;11(1):3-8, 2011 Jan.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Our objectives were to investigate the presence of Anaplasma spp. infection in red deer, wild boars, and Ixodes ricinus removed from deer surveyed in La Rioja, as well as to analyze the presence of Anaplasma spp. in I. ricinus from different Spanish regions--ours included. A total of 21 deer and 13 wild boar blood samples as well as 295 I. ricinus removed from deer, vegetation, or asymptomatic people were tested by polymerase chain reaction targeting Anaplasma spp. 16S rRNA gene and groESL heat shock operon. Twelve deer blood samples were found to be infected with Anaplasma centrale (n = 7) or Anaplasma phagocytophilum (n = 5). No wild boar blood samples gave positive polymerase chain reaction results. Further, A. phagocytophilum was detected in 12 out of 89 I. ricinus removed from deer and in 18 out of 168 I. ricinus collected over vegetation in the North of Spain. Anaplasma spp. was not detected in any of the 38 I. ricinus removed from people. Nucleotide sequences for 16S rRNA gene showed substancial heterogeneity. The etiological agent of human anaplasmosis was found in two deer blood samples, an adult tick from deer, and a nymph from vegetation. The 16S rRNA sequences for 12 out of 35 samples matched the sequence of the Ap-variant 1 strain previously described in the United States, and the remaining 19 positive samples (deer blood and I. ricinus) showed variations with unknown significance. Although the groEL DNA sequences varied among analyzed strains, the deduced amino acid sequences did not change for any of them. This study suggests that deer population from La Rioja harbors strains of A. phagocytophilum similar to that pathogen for humans and other of unknown pathogenicity. Further, it seems that the Ap-variant 1 is circulating among I. ricinus ticks from the North of Spain more frequently than the A. phagocytophilum strain associated to human anaplasmosis.
[Mh] Termos MeSH primário: Anaplasma centrale/isolamento & purificação
Anaplasma phagocytophilum/isolamento & purificação
Cervos/microbiologia
Ixodes/imunologia
Sus scrofa/microbiologia
[Mh] Termos MeSH secundário: Anaplasma centrale/classificação
Anaplasma centrale/genética
Anaplasma phagocytophilum/classificação
Anaplasma phagocytophilum/genética
Anaplasmose/microbiologia
Anaplasmose/transmissão
Animais
Vetores Aracnídeos/microbiologia
Proteínas de Bactérias/genética
Sequência de Bases
Chaperoninas/genética
Cervos/sangue
Cervos/parasitologia
Proteínas de Choque Térmico/genética
Seres Humanos
Ixodes/microbiologia
Dados de Sequência Molecular
Ninfa/microbiologia
Filogenia
Reação em Cadeia da Polimerase/veterinária
Polimorfismo Genético
RNA Ribossômico 16S/genética
Alinhamento de Sequência
Espanha
Sus scrofa/sangue
Sus scrofa/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (GroESL protein, Bacteria); 0 (Heat-Shock Proteins); 0 (RNA, Ribosomal, 16S); EC 3.6.1.- (Chaperonins)
[Em] Mês de entrada:1105
[Cu] Atualização por classe:110125
[Lr] Data última revisão:
110125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100610
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2009.0214


  8 / 19 MEDLINE  
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[PMID]:20036077
[Au] Autor:Molad T; Leibovich B; Mazuz M; Fleiderovich L; Fish L; Shkap V
[Ad] Endereço:Division of Parasitology, Kimron Veterinary Institute, P. O. Box 12, Bet Dagan 50250, Israel. moladt@int.gov.il
[Ti] Título:Identification of Anaplasma centrale major surface protein-2 pseudogenes.
[So] Source:Vet Microbiol;143(2-4):277-83, 2010 Jul 14.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study was aimed to identify msp2 pseudogenes and MSP2 variants in the vaccine Anaplama centrale strain. Five msp2 pseudogenes were identified in the A. centrale genome, and multiple MSP2 variants that emerged during both acute and persistent infection were detected. The pseudogene copies of msp2 were truncated; they contained a central hypervariable region flanked by short portions of the 5' and 3' conserved regions. Alignment of the hypervariable region sequence of the expression site of MSP2 variants with msp2 pseudogenes showed that MSP2 variants are generated by two mechanisms, previously described in Anaplasma marginale: (i) recombination of the whole pseudogene into the single msp2 expression site, and (ii) recombination of small segments of pseudogenes into the expression site by segmental gene conversion. The present study showed that the A. centrale MSP2 variants and the msp2 pseudogene repertoire were different from those reported for A. marginale. Unique MSP2 variants and pseudogenes identified in the vaccine strain allow the A. centrale-vaccinated cattle to be superinfected with the field strains of A. marginale. The knowledge gained in the present study on the mechanisms of antigenic variations in the vaccine strain of A. centrale is a further step in the development of a new generation vaccine against anaplasmosis.
[Mh] Termos MeSH primário: Anaplasma centrale/genética
Anaplasma centrale/metabolismo
Proteínas da Membrana Bacteriana Externa/genética
Pseudogenes/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Anaplasmose/microbiologia
Anaplasmose/prevenção & controle
Animais
Proteínas da Membrana Bacteriana Externa/química
Proteínas da Membrana Bacteriana Externa/metabolismo
Vacinas Bacterianas/imunologia
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/prevenção & controle
Regulação Bacteriana da Expressão Gênica/fisiologia
Genoma Bacteriano
Genômica
Dados de Sequência Molecular
Compostos Organometálicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (Bacterial Vaccines); 0 (Organometallic Compounds); 0 (RT-01 organotellurium compound); 0 (p44 protein, Anaplasma phagocytophila)
[Em] Mês de entrada:1011
[Cu] Atualização por classe:100524
[Lr] Data última revisão:
100524
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091229
[St] Status:MEDLINE
[do] DOI:10.1016/j.vetmic.2009.11.018


  9 / 19 MEDLINE  
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[PMID]:19967931
[Au] Autor:Palmer GH
[Ad] Endereço:Department of Veterinary Microbiology and Pathology and School for Global Animal Health, Washington State University, Pullman, Washington, USA 99164-7040.
[Ti] Título:Sir Arnold Theiler and the discovery of anaplasmosis: a centennial perspective.
[So] Source:Onderstepoort J Vet Res;76(1):75-9, 2009 Mar.
[Is] ISSN:0030-2465
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Sir Arnold Theiler's research in 1908/09 led to the discovery of the first rickettsial pathogen, Anaplasma marginale, and set the stage for his development and implementation of an effective live vaccine based on a less virulent strain, A. marginale ss. centrale. His 1910 report, describing A. marginale, is among the classic monographs in infectious disease research, presenting not only observations in exacting detail but also highlighting the deductive reasoning leading to association of a new pathogen with a specific disease. With a centennial perspective and both conceptual frameworks and molecular tools unimaginable in Theiler's time, the significance of several observations in the original report--cyclic bacteremia, strain superinfection, and taxonomic position--is now clear and highlight the broad applicability of key principles of pathogen biology.
[Mh] Termos MeSH primário: Anaplasma/classificação
Anaplasmose/história
Vacinas Bacterianas/história
Medicina Veterinária/história
[Mh] Termos MeSH secundário: Anaplasma/imunologia
Anaplasma/patogenicidade
Anaplasma centrale/classificação
Anaplasma centrale/imunologia
Anaplasma centrale/patogenicidade
Anaplasma marginale/classificação
Anaplasma marginale/imunologia
Anaplasma marginale/patogenicidade
Anaplasmose/microbiologia
Anaplasmose/prevenção & controle
Animais
História do Século XX
História do Século XXI
Seres Humanos
África do Sul
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE
[Ps] Nome de pessoa como assunto:Theiler A
[Nm] Nome de substância:
0 (Bacterial Vaccines)
[Em] Mês de entrada:1002
[Cu] Atualização por classe:091208
[Lr] Data última revisão:
091208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091209
[St] Status:MEDLINE


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[PMID]:19380205
[Au] Autor:Fletcher TI; Wigg JL; Rolls PJ; de Vos AJ
[Ad] Endereço:Tick Fever Centre, Department of Primary Industries and Fisheries, 280 Grindle Road, Wacol 4076, Australia.
[Ti] Título:Viability assays of intra-erythrocytic organisms using fluorescent dyes.
[So] Source:Vet Parasitol;163(1-2):144-7, 2009 Jul 07.
[Is] ISSN:0304-4017
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Three intra-erythrocytic tick fever organisms of cattle (Babesia bovis, Babesia bigemina and Anaplasma centrale) were subjected to a range of stressors, including heat, storage over time, specific chemotherapy and cryopreservation. Various stains, both alone and in combination, were used in an attempt to assess viability of these organisms before and after the stressors were applied. Carboxyfluorescein diacetate succinimidyl ester (CFSE) stained live Babesia spp. very well while fluorescein diacetate (FDA) stained A. centrale successfully. Propidium iodide (PI) and ethidium-homodimer-1 (Eth-D) were used as counter stains to identify dead organisms. Stain combinations allowed differentiation between living and dead Babesia organisms after exposure to heat and after chemotherapy. PI and Eth-D as counter stains were of little value after deglycerolisation of cryopreserved organisms. Possible reasons for this limited success in determining death or viability of tick fever organisms after some treatments include the impermeability of red blood cells to PI and Eth-D counter stains or the loss of live and/or dead organisms during sample processing.
[Mh] Termos MeSH primário: Anaplasma centrale/citologia
Babesia/citologia
Doenças dos Bovinos/sangue
Eritrócitos/parasitologia
[Mh] Termos MeSH secundário: Anaplasma centrale/efeitos dos fármacos
Anaplasma centrale/fisiologia
Anaplasmose/sangue
Animais
Antiprotozoários/uso terapêutico
Babesia/efeitos dos fármacos
Babesia/fisiologia
Babesiose/parasitologia
Babesiose/veterinária
Bovinos
Doenças dos Bovinos/tratamento farmacológico
Criopreservação
Corantes Fluorescentes
Temperatura Alta
Manejo de Espécimes
Coloração e Rotulagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Fluorescent Dyes)
[Em] Mês de entrada:0909
[Cu] Atualização por classe:090615
[Lr] Data última revisão:
090615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090422
[St] Status:MEDLINE
[do] DOI:10.1016/j.vetpar.2009.03.029



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