Base de dados : MEDLINE
Pesquisa : B03.440.040.050.500 [Categoria DeCS]
Referências encontradas : 310 [refinar]
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[PMID]:28169172
[Au] Autor:Sisson D; Hufschmid J; Jolles A; Beechler B; Jabbar A
[Ad] Endereço:Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria, Australia. Electronic address: dsisson@student.unimelb.edu.au.
[Ti] Título:Molecular characterisation of Anaplasma species from African buffalo (Syncerus caffer) in Kruger National Park, South Africa.
[So] Source:Ticks Tick Borne Dis;8(3):400-406, 2017 Mar.
[Is] ISSN:1877-9603
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bovine anaplasmosis is a tick-borne disease, mainly caused by Anaplasma marginale and A. centrale and is distributed in tropical and sub-tropical areas. This study aimed to characterise A. marginale and A. centrale from African buffaloes in Kruger National Park (KNP), South Africa, using the DNA sequences of the genes coding for major surface protein (msp1ß) and heat shock protein (groEL), respectively. A total of 747 blood samples were collected from February 2014 to August 2016 from African buffaloes kept in KNP, and DNAs were tested using a molecular-phylogenetic approach. Out of 747 samples tested, 129 (17.3%) and 98 (13.1%) were positive for single infection with A. marginale and A. centrale, respectively; whereas 113 (15.1%) were positive for both Anaplasma spp. Pairwise difference of 1.6-8.5% was observed in msp1ß sequences of A. marginale whereas that was only 0.3-2.4% for groEL sequences of A. centrale. Separate phylogenetic analyses of msp1ß and groEL sequences of A. marginale and A. centrale, respectively, revealed that sequences of Anaplasma spp. from African buffaloes were unique and they grouped separately when compared with previously published sequences of both species. This is the first study to characterise A. marginale and A. centrale from African buffalo using species specific molecular markers. This study will pave the way for future studies to assess genetic variation among Anaplasma spp. from wild ruminants using molecular markers that are better at differentiating between species and strains than the more commonly used 16S rRNA gene, and help to undertake health and fitness studies and host-parasite dynamics using quantitative molecular tools.
[Mh] Termos MeSH primário: Anaplasma marginale/genética
Anaplasma/genética
Anaplasma/isolamento & purificação
Anaplasmose/epidemiologia
Búfalos/microbiologia
Doenças dos Bovinos/microbiologia
[Mh] Termos MeSH secundário: Anaplasma/classificação
Anaplasma marginale/classificação
Anaplasma marginale/isolamento & purificação
Anaplasmose/microbiologia
Animais
Proteínas da Membrana Bacteriana Externa/genética
Bovinos
Doenças dos Bovinos/epidemiologia
Chaperonina 60/genética
DNA Bacteriano/genética
Variação Genética
Parques Recreativos
Filogenia
RNA Ribossômico 16S/genética
África do Sul/epidemiologia
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (Chaperonin 60); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (major surface protein 1b, Anaplasma)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE


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[PMID]:28155283
[Au] Autor:Chaisi ME; Baxter JR; Hove P; Choopa CN; Oosthuizen MC; Brayton KA; Khumalo ZT; Mutshembele AM; Mtshali MS; Collins NE
[Ad] Endereço:Department of Veterinary Tropical Diseases, University of Pretoria. mechaisi@yahoo.co.uk.
[Ti] Título:Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle.
[So] Source:Onderstepoort J Vet Res;84(1):e1-e9, 2017 Jan 23.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Several nucleic acid-based assays have been developed for detecting Anaplasma marginale and Anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas difficult. We evaluated the ability of the reverse line blot (RLB) hybridisation assay, two nested polymerase chain reaction (nPCR) assays and a duplex real-time quantitative polymerase chain reaction (qPCR) assay to detect A. marginale and A. centrale infections in cattle (n = 66) in South Africa. The lowest detection limits for A. marginale plasmid DNA were 2500 copies by the RLB assay, 250 copies by the nPCR and qPCR assays and 2500, 250 and 25 copies of A. centrale plasmid DNA by the RLB, nPCR and qPCR assays respectively. The qPCR assay detected more A. marginale- and A. centrale-positive samples than the other assays, either as single or mixed infections. Although the results of the qPCR and nPCR tests were in agreement for the majority (38) of A. marginale-positive samples, 13 samples tested negative for A. marginale using nPCR but positive using qPCR. To explain this discrepancy, the target sequence region of the nPCR assay was evaluated by cloning and sequencing the msp1ß gene from selected field samples. The results indicated sequence variation in the internal forward primer (AM100) area amongst the South African A. marginale msp1ß sequences, resulting in false negatives. We propose the use of the duplex qPCR assay in future studies as it is more sensitive and offers the benefits of quantification and multiplex detection of both Anaplasma spp.
[Mh] Termos MeSH primário: Anaplasma centrale
Anaplasma marginale
Anaplasmose/diagnóstico
Doenças dos Bovinos/diagnóstico
Hibridização de Ácido Nucleico
Reação em Cadeia da Polimerase/veterinária
Reação em Cadeia da Polimerase em Tempo Real/veterinária
[Mh] Termos MeSH secundário: Anaplasma centrale/genética
Anaplasma marginale/genética
Anaplasmose/microbiologia
Animais
Bovinos
Doenças dos Bovinos/microbiologia
DNA Bacteriano/genética
Hibridização de Ácido Nucleico/métodos
Reação em Cadeia da Polimerase/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v84i1.1262


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[PMID]:28122249
[Au] Autor:Battilani M; De Arcangeli S; Balboni A; Dondi F
[Ad] Endereço:Department of Veterinary Medical Sciences, Alma Mater Studiorum - University of Bologna, Via Tolara di Sopra, 50, 40064 Ozzano Emilia, Bo, Italy. Electronic address: mara.battilani@unibo.it.
[Ti] Título:Genetic diversity and molecular epidemiology of Anaplasma.
[So] Source:Infect Genet Evol;49:195-211, 2017 Apr.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Anaplasma are obligate intracellular bacteria of cells of haematopoietic origin and are aetiological agents of tick-borne diseases of both veterinary and medical interest common in both tropical and temperate regions. The recent disclosure of their zoonotic potential has greatly increased interest in the study of these bacteria, leading to the recent reorganisation of Rickettsia taxonomy and to the possible discovery of new species belonging to the genus Anaplasma. This review is particularly focused on the common and unique characteristics of Anaplasma marginale and Anaplasma phagocytophilum, with an emphasis on genetic diversity and evolution, and the main distinguishing features of the diseases caused by the different Anaplasma spp. are described as well.
[Mh] Termos MeSH primário: Anaplasma marginale/genética
Anaplasma/genética
Anaplasmose/epidemiologia
Doenças dos Bovinos/epidemiologia
Filogenia
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Anaplasma/classificação
Anaplasma marginale/classificação
Anaplasmose/microbiologia
Anaplasmose/transmissão
Animais
Antígenos de Bactérias/genética
Evolução Biológica
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/transmissão
DNA Bacteriano/genética
Variação Genética
Epidemiologia Molecular
Filogeografia
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/microbiologia
Doenças Transmitidas por Carrapatos/transmissão
Carrapatos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (DNA, Bacterial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE


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[PMID]:28028631
[Au] Autor:Jirapattharasate C; Adjou Moumouni PF; Cao S; Iguchi A; Liu M; Wang G; Zhou M; Vudriko P; Efstratiou A; Changbunjong T; Sungpradit S; Ratanakorn P; Moonarmart W; Sedwisai P; Weluwanarak T; Wongsawang W; Suzuki H; Xuan X
[Ad] Endereço:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
[Ti] Título:Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand.
[So] Source:Parasitol Res;116(2):751-762, 2017 Feb.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Babesia spp., Theileria orientalis, and Anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. In this study, we used PCR to detect the presence of Babesia bovis, Babesia bigemina, and T. orientalis in 279 beef cattle from Western Thailand and A. marginale in 608 beef cattle from the north, northeastern, and western regions. The PCRs were performed using species-specific primers based on the B. bovis spherical body protein 2 (BboSBP2), B. bigemina rhoptry-associated protein 1a (BbiRAP-1a), T. orientalis major piroplasm surface protein (ToMPSP), and A. marginale major surface protein 4 (AmMSP4) genes. To determine the genetic diversity of the above parasites, amplicons of B. bovis and B. bigemina ITS1-5.8s rRNA gene-ITS2 regions (B. bovis ITS, B. bigemina ITS), ToMPSP, and AmMSP4 genes were sequenced for phylogenetic analysis. PCR results revealed that the prevalence of B. bovis, B. bigemina, T. orientalis, and A. marginale in the Western region was 11.1, 12.5, 7.8, and 39.1 %, respectively. Coinfections of two or three parasites were observed in 17.9 % of the animals sampled. The study revealed that the prevalence of A. marginale in the western region was higher than in the north and northeastern regions (7 %). Sequence analysis showed the BboSBP2 gene to be more conserved than B. bovis ITS in the different isolates and, similarly, the BbiRAP-1a was more conserved than B. bigemina ITS. In the phylogenetic analysis, T. orientalis MPSP sequences were classified into types 3, 5, and 7 as previously reported. A. marginale MSP4 gene sequences shared high identity and similarity with each other and clustered with isolates from other countries. This study provides information on the prevalence and genetic diversity of tick-borne pathogens in beef cattle and highlights the need for effective strategies to control these pathogens in Thailand.
[Mh] Termos MeSH primário: Anaplasmose/microbiologia
Babesiose/parasitologia
Doenças dos Bovinos
Variação Genética
Theileriose/parasitologia
[Mh] Termos MeSH secundário: Anaplasma marginale/genética
Anaplasma marginale/isolamento & purificação
Anaplasmose/epidemiologia
Animais
Babesia/genética
Babesia/isolamento & purificação
Babesia bovis/genética
Babesia bovis/isolamento & purificação
Babesiose/epidemiologia
Sequência de Bases
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/parasitologia
Primers do DNA/genética
DNA de Protozoário/química
DNA de Protozoário/genética
Geografia
Filogenia
Reação em Cadeia da Polimerase/veterinária
Alinhamento de Sequência/veterinária
Análise de Sequência de DNA/veterinária
Tailândia/epidemiologia
Theileria/genética
Theileria/isolamento & purificação
Theileriose/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Protozoan)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-016-5345-2


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[PMID]:27993973
[Au] Autor:Hebert KS; Seidman D; Oki AT; Izac J; Emani S; Oliver LD; Miller DP; Tegels BK; Kannagi R; Marconi RT; Carlyon JA
[Ad] Endereço:Department of Microbiology and Immunology, Virginia Commonwealth University Medical Center, School of Medicine, Richmond, Virginia, USA.
[Ti] Título:Anaplasma marginale Outer Membrane Protein A Is an Adhesin That Recognizes Sialylated and Fucosylated Glycans and Functionally Depends on an Essential Binding Domain.
[So] Source:Infect Immun;85(3), 2017 Mar.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:causes bovine anaplasmosis, a debilitating and potentially fatal tick-borne infection of cattle. Because is an obligate intracellular organism, its adhesins that mediate entry into host cells are essential for survival. Here, we demonstrate that outer membrane protein A (AmOmpA; AM854) contributes to the invasion of mammalian and tick host cells. AmOmpA exhibits predicted structural homology to OmpA of (ApOmpA), an adhesin that uses key lysine and glycine residues to interact with α2,3-sialylated and α1,3-fucosylated glycan receptors, including 6-sulfo-sialyl Lewis x (6-sulfo-sLe ). Antisera against AmOmpA or its predicted binding domain inhibits infection of host cells. Residues G55 and K58 are contributory, and K59 is essential for recombinant AmOmpA to bind to host cells. Enzymatic removal of α2,3-sialic acid and α1,3-fucose residues from host cell surfaces makes them less supportive of AmOmpA binding. AmOmpA is both an adhesin and an invasin, as coating inert beads with it confers adhesiveness and invasiveness. Recombinant forms of AmOmpA and ApOmpA competitively antagonize infection of host cells, but a monoclonal antibody against 6-sulfo-sLe fails to inhibit AmOmpA adhesion and infection. Thus, the two OmpA proteins bind related but structurally distinct receptors. This study provides a detailed understanding of AmOmpA function, identifies its essential residues that can be targeted by blocking antibody to reduce infection, and determines that it binds to one or more α2,3-sialylated and α1,3-fucosylated glycan receptors that are unique from those targeted by ApOmpA.
[Mh] Termos MeSH primário: Adesinas Bacterianas/metabolismo
Anaplasma marginale/fisiologia
Proteínas da Membrana Bacteriana Externa/metabolismo
Polissacarídeos/metabolismo
Domínios e Motivos de Interação entre Proteínas
[Mh] Termos MeSH secundário: Adesinas Bacterianas/química
Motivos de Aminoácidos
Aminoácidos
Animais
Proteínas da Membrana Bacteriana Externa/química
Proteínas da Membrana Bacteriana Externa/imunologia
Sítios de Ligação
Linhagem Celular
Fucose/metabolismo
Soros Imunes/imunologia
Modelos Moleculares
Conformação Molecular
Ácido N-Acetilneuramínico/metabolismo
Polissacarídeos/química
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adhesins, Bacterial); 0 (Amino Acids); 0 (Bacterial Outer Membrane Proteins); 0 (Immune Sera); 0 (Polysaccharides); 149024-69-1 (OMPA outer membrane proteins); 28RYY2IV3F (Fucose); GZP2782OP0 (N-Acetylneuraminic Acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161221
[St] Status:MEDLINE


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[PMID]:27939332
[Au] Autor:George N; Bhandari V; Sharma P
[Ad] Endereço:National Institute of Animal Biotechnology-DBT, Hyderabad, India.
[Ti] Título:Phylogenetic relationship and genotypic variability in Anaplasma marginale strains causing anaplasmosis in India.
[So] Source:Infect Genet Evol;48:71-75, 2017 Mar.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Anaplasma marginale is a tick borne rickesttsial parasite known to cause bovine anaplasmosis. There are prevalence reports from different parts of India, however, information regarding genetic diversity and phylogenetic association of the Indian strains are unknown. In the current study, 965 cattle blood samples from two states of India, Seemandhra and Telangana, were investigated for the presence of A. marginale by PCR using major surface protein 4 gene (msp4). We found an overall infection of 16.4%, with 3.4% prevalence in Seemandhra and 22.2% in Telangana. Sequence analysis of the 24 cloned msp4 gene indicated genetic diversity among Indian clinical strains of A. marginale which may be due to evolutionary pressure or migration of strains. Phylogenetic association analysis revealed that most of the strains showed close proximity with strains from Mexico and other strains showed closeness to strains reported from countries like Brazil, Zimbabwe, Prico and Hungary. This is the first report from India, identifying heterogeneous population of A. marginale strains causing anaplasmosis, and such data can play an important role in designing new control policies.
[Mh] Termos MeSH primário: Anaplasma marginale/genética
Anaplasmose/microbiologia
Doenças dos Bovinos/microbiologia
[Mh] Termos MeSH secundário: Anaplasmose/epidemiologia
Animais
Proteínas de Bactérias/genética
Bovinos
Doenças dos Bovinos/epidemiologia
Genes Bacterianos
Variação Genética
Genótipo
Índia/epidemiologia
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27878428
[Au] Autor:Wesonga FD; Gachohi JM; Kitala PM; Gathuma JM; Njenga MJ
[Ad] Endereço:Kenya Agricultural Research Institute (KARI), Muguga South, P.O. Box 32, Kikuyu, Kenya.
[Ti] Título:Seroprevalence of Anaplasma marginale and Babesia bigemina infections and associated risk factors in Machakos County, Kenya.
[So] Source:Trop Anim Health Prod;49(2):265-272, 2017 Feb.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Anaplasma marginale and Babesia bigemina are important tick-borne pathogens of cattle. A cross-sectional survey was undertaken to determine the seroprevalence of A. marginale and B. bigemina infections and identify associated risk factors on traditional smallholder farms in Machakos County, Kenya. A total of 421 cattle from 127 farms from four divisions in the county were sampled and visited between September and November 2007. The farms were selected by a proportional allocation approach based on the number of farms in the four divisions previously selected by stratified random sampling method. Information on animal and individual farm management variables was obtained using standardized questionnaires. Prevalence of serum antibodies due to A. marginale and B. bigemina pathogens was determined using the enzyme-linked immunosorbent assay (ELISA) technique. The relationship between the seropositivity and associated risk factors was assessed by multivariable analyses using standard logistic regression models. The overall estimation (and their 95% confidence intervals) of A. marginale and B. bigemina seropositivity at the animal level was 53.4% (48.5%, 58.2%) and 40.6% (35.8%, 45.4%), respectively. Two variables, "animal age" and "administrative division," were significantly associated with the A. marginale seroresponse. Three variables, "animal age" "grazing system" and "administrative division" were significantly associated with the B. bigemina seroresponse. These findings suggest possible indicators of existence of endemic instability for the two infections. The study identifies characterization of environmental suitability for the vectors and how they interact with grazing systems to cause the infections as an area for further studies, for improved understanding of the infections and in designing disease control programs.
[Mh] Termos MeSH primário: Anaplasma marginale/isolamento & purificação
Anaplasmose/epidemiologia
Babesia/isolamento & purificação
Babesiose/epidemiologia
Doenças dos Bovinos/epidemiologia
[Mh] Termos MeSH secundário: Anaplasmose/microbiologia
Animais
Babesiose/parasitologia
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/parasitologia
Estudos Transversais
Ensaio de Imunoadsorção Enzimática/veterinária
Feminino
Quênia/epidemiologia
Modelos Logísticos
Masculino
Prevalência
Fatores de Risco
Estudos Soroepidemiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-016-1187-2


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[PMID]:27795302
[Au] Autor:Deringer JR; Forero-Becerra EG; Ueti MW; Turse JE; Futse JE; Noh SM; Palmer GH; Brown WC
[Ad] Endereço:Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
[Ti] Título:Identification of a T-Cell Epitope That Is Globally Conserved among Outer Membrane Proteins (OMPs) OMP7, OMP8, and OMP9 of Anaplasma marginale Strains and with OMP7 from the A. marginale subsp. centrale Vaccine Strain.
[So] Source:Clin Vaccine Immunol;24(1), 2017 Jan.
[Is] ISSN:1556-679X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Within the protective outer membrane (OM) fraction of Anaplasma marginale, several vaccine candidates have emerged, including a family of OM proteins (OMPs) 7 to 9, which share sequence identity with each other and with the single protein OMP7 in the vaccine strain A. marginale subsp. centrale. A. marginale OMPs 7 to 9 are logical vaccine candidates because they are surface exposed, present in the OM immunogen and protective cross-linked OM proteins, recognized by immune serum IgG2 and T cells in cattle immunized with OM, and recognized by immune serum IgG2 from cattle immunized with the A. centrale vaccine strain. We report the identification of a globally conserved 9-amino-acid T-cell epitope FLLVDDAI/VV shared between A. centrale vaccine strain OMP7 and the related A. marginale OMPs 7 to 9, where position 8 of the peptide can be isoleucine or valine. The epitope is conserved in American A. marginale strains, in the Australia Gypsy Plains strain, and in multiple field isolates from Ghana. This epitope, together with additional T-cell epitopes that are present within these proteins, should be considered for inclusion in a multivalent vaccine for A. marginale that can provide protection against disease caused by globally distributed bacterial strains.
[Mh] Termos MeSH primário: Anaplasma marginale/imunologia
Proteínas da Membrana Bacteriana Externa/imunologia
Sequência Conservada
Epitopos de Linfócito T/imunologia
[Mh] Termos MeSH secundário: Américas
Anaplasma marginale/isolamento & purificação
Animais
Austrália
Gana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (Epitopes, T-Lymphocyte)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE


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[PMID]:27787614
[Au] Autor:Elelu N; Ferrolho J; Couto J; Domingos A; Eisler MC
[Ad] Endereço:School of Veterinary Science, University of Bristol, Langford, Bristol, BS40 5DU, UK.
[Ti] Título:Molecular diagnosis of the tick-borne pathogen Anaplasma marginale in cattle blood samples from Nigeria using qPCR.
[So] Source:Exp Appl Acarol;70(4):501-510, 2016 Dec.
[Is] ISSN:1572-9702
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Tick-borne diseases (TBDs) are some of the most important animal health and management problems in Africa, including Nigeria. This study aims to determine the prevalence of an important TBD, anaplasmosis, in a North-central region of Nigeria. Blood samples were collected from cattle and stored on Whatman FTA cards. Information on village, age and sex associated with each cattle was also recorded. The packed red blood cell volume (PCV) for each blood sample was determined. After DNA extraction, pathogen presence was evaluated by TaqMan based qPCR of which 75.9 % of the cattle tested positive for Anaplasma marginale. Statistical analysis revealed that the presence of A. marginale infection differed significantly between cattle age groups. However, there was no significant difference in the prevalence of this pathogen between the sexes or among cattle grouped by PCV level. Finally, using a highly sensitive molecular method our pioneer study contributes to the improvement of the current knowledge regarding tick-borne pathogens that seriously affect animal health in specific areas of Nigeria.
[Mh] Termos MeSH primário: Anaplasma marginale/fisiologia
Anaplasmose/diagnóstico
Doenças dos Bovinos/diagnóstico
[Mh] Termos MeSH secundário: Anaplasmose/epidemiologia
Anaplasmose/microbiologia
Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/microbiologia
DNA Bacteriano/genética
Nigéria/epidemiologia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Análise de Sequência de DNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161028
[St] Status:MEDLINE


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[PMID]:27765073
[Au] Autor:M'ghirbi Y; Bèji M; Oporto B; Khrouf F; Hurtado A; Bouattour A
[Ad] Endereço:Université Tunis El Manar, Institut Pasteur de Tunis, Laboratoire d'Epidémiologie et de Microbiologie Vétérinaire, Service d'Entomologie Médicale, 1002, Tunis-Belvédère, Tunisia.
[Ti] Título:Anaplasma marginale and A. phagocytophilum in cattle in Tunisia.
[So] Source:Parasit Vectors;9(1):556, 2016 Oct 20.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tick-borne diseases caused by Anaplasma species put serious constraints on the health and production of domestic cattle in tropical and sub-tropical regions. After recovering from a primary infection, cattle typically become persistent carriers of pathogens and play a critical role in the epidemiology of the disease, acting as reservoirs of the Anaplasma spp. METHODS: In this study a duplex PCR assay was used for the simultaneous detection of Anaplasma marginale and Anaplasma phagocytophilum in cattle using two primer pairs targeting msp4 and msp2 genes, respectively. We used this method to analyze DNA preparations derived from 328 blood cattle samples that were collected from 80 farms distributed among Tunisia's four bioclimatic zones. RESULTS: The prevalence of the A. marginale infection (24.7 %) was significantly higher and more widespread (in all bioclimatic areas) than that of A. phagocytophilum (0.6 %), which was found in a mixed infection with A. marginale. CONCLUSIONS: The duplex PCR assay used proved to be a rapid, specific and inexpensive mean for the simultaneous detection of Anaplasma marginale and Anaplasma phagocytophilum in cattle blood. It allowed us to report the identification of A. phagocytophilum for the first time in cattle in Tunisia and confirm the presence of A. marginale in cattle from several geographical areas of the country. Further epidemiological studies undertaken using this assay will help improve the surveillance of the associated diseases in the regions where they are endemic.
[Mh] Termos MeSH primário: Anaplasma marginale/isolamento & purificação
Anaplasma phagocytophilum/isolamento & purificação
Anaplasmose/epidemiologia
Doenças dos Bovinos/epidemiologia
[Mh] Termos MeSH secundário: Anaplasma marginale/genética
Anaplasma phagocytophilum/genética
Anaplasmose/microbiologia
Animais
Sangue/microbiologia
Bovinos
Doenças dos Bovinos/microbiologia
Reação em Cadeia da Polimerase Multiplex
Prevalência
Análise de Sequência de DNA
Tunísia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161022
[St] Status:MEDLINE



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