Base de dados : MEDLINE
Pesquisa : B03.440.040.050.575 [Categoria DeCS]
Referências encontradas : 25 [refinar]
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  1 / 25 MEDLINE  
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[PMID]:28131376
[Au] Autor:Aouadi A; Leulmi H; Boucheikhchoukh M; Benakhla A; Raoult D; Parola P
[Ad] Endereço:Université Chadli Bendjdid, Département des Sciences Vétérinaires, El Tarf, 36000, Algeria; Université Mohamed Cherif Messaadia, Institut des Sciences Agronomiques et Vétérinaires, Souk Ahras, 41000, Algeria.
[Ti] Título:Molecular evidence of tick-borne hemoprotozoan-parasites (Theileria ovis and Babesia ovis) and bacteria in ticks and blood from small ruminants in Northern Algeria.
[So] Source:Comp Immunol Microbiol Infect Dis;50:34-39, 2017 Feb.
[Is] ISSN:1878-1667
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Using qPCR, standard PCR and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from Souk Ahras, Algeria. Borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. Anaplasma ovis was screened in (38/73, 52%) Rhipicephalus bursa and (5/22, 22.7%) R. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. Coxiella burnetii tested positive in R. bursa (4/73, 5.5%) and (7/120, 5.8%) blood of sheep and (2/120, 1.7%) goats. Theileria ovis was detected in (50/147, 34%) R. bursa and (3/22, 13.6%) R. turanicus and in (64/120, 53.3%) blood of sheep and (25/120, 20.8%) goats. Babesia ovis was screened positive in (23/147, 15.6%) R. bursa and (7/48, 14.6%) R. turanicus. Our findings expand knowledge about the repertoire of tick-borne microorganisms present in ectoparasites and/or the blood of small ruminants in Algeria.
[Mh] Termos MeSH primário: Bactérias Gram-Negativas/isolamento & purificação
Piroplasmida/isolamento & purificação
Rhipicephalus/microbiologia
Rhipicephalus/parasitologia
Ruminantes/microbiologia
Ruminantes/parasitologia
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Argélia/epidemiologia
Anaplasma ovis/genética
Anaplasma ovis/isolamento & purificação
Anaplasmose/diagnóstico
Anaplasmose/epidemiologia
Anaplasmose/microbiologia
Animais
Babesia/genética
Babesia/isolamento & purificação
Babesiose/diagnóstico
Babesiose/epidemiologia
Babesiose/parasitologia
Borrelia/genética
Borrelia/isolamento & purificação
Infecções por Borrelia/diagnóstico
Infecções por Borrelia/epidemiologia
Infecções por Borrelia/microbiologia
Coxiella burnetii/genética
Coxiella burnetii/isolamento & purificação
Estudos Transversais
Doenças das Cabras/diagnóstico
Doenças das Cabras/microbiologia
Doenças das Cabras/parasitologia
Cabras/parasitologia
Bactérias Gram-Negativas/genética
Piroplasmida/genética
Reação em Cadeia da Polimerase/veterinária
Febre Q/diagnóstico
Febre Q/epidemiologia
Febre Q/microbiologia
Febre Q/veterinária
Ovinos/parasitologia
Doenças dos Ovinos/diagnóstico
Doenças dos Ovinos/microbiologia
Doenças dos Ovinos/parasitologia
Theileria/genética
Theileria/isolamento & purificação
Theileriose/diagnóstico
Theileriose/epidemiologia
Theileriose/parasitologia
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/microbiologia
Doenças Transmitidas por Carrapatos/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170217
[Lr] Data última revisão:
170217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170130
[St] Status:MEDLINE


  2 / 25 MEDLINE  
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[PMID]:27142027
[Au] Autor:Jalali SM; Bahrami S; Rasooli A; Hasanvand S
[Ad] Endereço:Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. mi.jalali@scu.ac.ir.
[Ti] Título:Evaluation of oxidant/antioxidant status, trace mineral levels, and erythrocyte osmotic fragility in goats naturally infected with Anaplasma ovis.
[So] Source:Trop Anim Health Prod;48(6):1175-81, 2016 Aug.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Anaplasma ovis, an arthropod-borne pathogen that infects erythrocytes, is the major cause of ovine and caprine anaplasmosis. This study was performed to assess in goats infected with A. ovis the osmotic fragility of erythrocytes, antioxidant status, and serum levels of microminerals. Blood samples were collected from 104 mixed breed goats in Ahvaz area, southwest Iran and subjected to parasitologic, hematologic, oxidant/antioxidant, and micromineral assessment. Anaplasma infection was detected in 30 samples (28.8 %) by microscopic examination of blood smears while PCR-RFLP analysis revealed infection with A. ovis in 68 samples (65.4 %). Studied animals were divided into three groups based on A. ovis infection: Uninfected goats as control group (group 1), PCR positive without parasitemia (group 2) and PCR positive with parasitemia (group 3). Hematological evaluation showed significantly increased lymphocyte and monocyte counts in Anaplasma-infected groups (group 2 and 3). A significantly lower MCHC and higher MCV were also observed in infected groups. In group 3 significant rises in erythrocyte's osmotic fragility in different salt concentrations and also in median corpuscular fragility (MCF) was seen. Evaluation of the antioxidant defense system of the erythrocytes revealed a decrease in total antioxidant capacity (TAC) and superoxide dismutase (SOD) activity in group 3. There was no significant difference in serum micromineral levels between infected and uninfected animals. Overall, the observed substantial decrease in the antioxidant enzyme activities with remarkable elevated levels of erythrocyte osmotic fragility indicate high exposure of erythrocytes to oxidative damage in Anaplasma-infected goats. These results also suggest that the disturbed antioxidant defense mechanisms in caprine anaplasmosis can promote the development of anemia.
[Mh] Termos MeSH primário: Anaplasma ovis/patogenicidade
Anaplasmose/microbiologia
[Mh] Termos MeSH secundário: Anaplasmose/sangue
Animais
Antioxidantes/metabolismo
Eritrócitos/fisiologia
Doenças das Cabras/sangue
Doenças das Cabras/microbiologia
Cabras
Irã (Geográfico)
Fragilidade Osmótica
Oxidantes/sangue
Estações do Ano
Oligoelementos/sangue
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Oxidants); 0 (Trace Elements)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160505
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-016-1071-0


  3 / 25 MEDLINE  
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[PMID]:25660960
[Au] Autor:Li Y; Chen Z; Liu Z; Liu J; Yang J; Li Q; Li Y; Luo J; Yin H
[Ad] Endereço:State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China. youquan-li@163.com, liyouquan@caas.cn.
[Ti] Título:Molecular Survey of Anaplasma and Ehrlichia of Red Deer and Sika Deer in Gansu, China in 2013.
[So] Source:Transbound Emerg Dis;63(6):e228-e236, 2016 Dec.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Anaplasma and Ehrlichia are important emerging tick-borne pathogens in both humans and animals. Here, we conducted a molecular surveillance study in Gansu, China to assess the prevalence of Anaplasma and Ehrlichia spp. in red deer and sika deer based on polymerase chain reaction (PCR) analysis and sequencing of 16S rRNA or msp genes. PCR revealed that the prevalence of Anaplasma ovis, Anaplasma bovis and Anaplasma platys of the Qilian Mountain samples was 32%, 9% and 9%, respectively; the prevalence of Anaplasma ovis, Anaplasma bovis, Anaplasma platys was 20%, 15% and 15% among the Long Mountain samples, respectively. Of the Long Mountain samples, two (5%) of the 40 samples were positive for Ehrlichia canis, but all 44 of the Qilian Mountain samples were negative for E. canis, and no other Anaplasma or Ehrlichia spp. were found in the samples. The phylogenetic tree showed that the newly isolated Anaplasma and Ehrlichia spp. could be classified as belonging to four clades, including an A. bovis cluster, A. ovis cluster, A. platys cluster and E. canis cluster. In addition, Bartonella schoenbuchensis was firstly identified in blood samples from red deer in Gansu, China. Our results provide important data to increase the understanding of the epidemiology of anaplasmosis and ehrlichiosis of red deer and sika deer and will assist with the implementation of measures to control anaplasmosis and ehrlichiosis transmission to red deer, sika deer and other animals in Gansu, China.
[Mh] Termos MeSH primário: Anaplasma/classificação
Anaplasmose/epidemiologia
Cervos/microbiologia
Ehrlichia/classificação
Ehrlichiose/veterinária
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Anaplasma/genética
Anaplasma ovis/classificação
Anaplasma ovis/genética
Anaplasmose/microbiologia
Animais
Vetores Aracnídeos/microbiologia
China/epidemiologia
Ehrlichia/genética
Ehrlichia canis/classificação
Ehrlichia canis/genética
Ehrlichiose/epidemiologia
Ehrlichiose/microbiologia
Seres Humanos
Filogenia
Análise de Sequência de DNA/veterinária
Inquéritos e Questionários
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/microbiologia
Carrapatos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150210
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12335


  4 / 25 MEDLINE  
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[PMID]:26455373
[Au] Autor:Giadinis N; Katsoulos P; Chochlakis D; Tselentis Y; Ntais P; Lafi S; Karatzias H; Psaroulaki A
[Ad] Endereço:Clinic of Farm Animals, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 546 27 Thessaloniki, Greece.
[Ti] Título:Serological investigation for West Nile virus, Anaplasma ovis and Leishmania infantum in Greek cattle.
[So] Source:Vet Ital;51(3):205-9, 2015 Jul-Sep.
[Is] ISSN:1828-1427
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:This study investigates the seroprevalence of Greek adult cattle against West Nile virus, Anaplasma ovis and Lehismania infantum. In total, 156 serum samples were examined, drawn from cattle between 2-4 years old. All the examined cattle originated from slaughterhouses of 4 prefectures in Northern Greece (Thessaloniki, Pella, Chalkidiki, Kilkis), in 2 of which (Thessaloniki, Pella) human cases of West Nile virus had been recorded some months before. Thirty out of the 156 (18.6%) samples have tested positive for West Nile virus and fifty-five (35.9%) samples for Anaplasma ovis. All the examined samples tested negative for Leishmania infantum. The prefectures with positive samples against West Nile virus also showed human cases of West Nile virus infections. This should raise questions whether cattle could become markers for West Nile virus activity in high risk areas.
[Mh] Termos MeSH primário: Anaplasma ovis/imunologia
Anticorpos Antibacterianos/sangue
Anticorpos Antiprotozoários/sangue
Anticorpos Antivirais/sangue
Doenças dos Bovinos/sangue
Doenças dos Bovinos/epidemiologia
Leishmania infantum/imunologia
Vírus do Nilo Ocidental/imunologia
[Mh] Termos MeSH secundário: Anaplasmose/sangue
Anaplasmose/epidemiologia
Animais
Bovinos
Grécia/epidemiologia
Leishmaniose Visceral/sangue
Leishmaniose Visceral/epidemiologia
Leishmaniose Visceral/veterinária
Estudos Soroepidemiológicos
Febre do Nilo Ocidental/sangue
Febre do Nilo Ocidental/epidemiologia
Febre do Nilo Ocidental/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antibodies, Protozoan); 0 (Antibodies, Viral)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151012
[Lr] Data última revisão:
151012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151013
[St] Status:MEDLINE
[do] DOI:10.12834/VetIt.174.523.4


  5 / 25 MEDLINE  
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[PMID]:24589111
[Au] Autor:Chi Q; Liu Z; Li Y; Yang J; Chen Z; Yue C; Luo J; Yin H
[Ad] Endereço:State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, China.
[Ti] Título:Development of a real-time PCR assay for detection and quantification of Anaplasma ovis infection.
[So] Source:Transbound Emerg Dis;60 Suppl 2:119-24, 2013 Nov.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Anaplasma ovis is a tick-borne intra-erythrocytic rickettsial pathogen of small ruminants. Real-time PCR possesses merits of rapidity, accuracy, reliability, automation and ease of standardization, but has not been used for detection of A. ovis, to the best of our knowledge. In this study, a real-time PCR assay was developed for detection and quantification of A. ovis. Species-specific primers and TaqMan probe were designed based on the gltA gene. No cross-reactions were observed with Anaplasma marginale, Anaplasma bovis, Anaplasma phagocytophilum, Borrelia burgdorferi s. l., Chlamydia psittaci, Mycoplasma mycoides, Theileria luwenshuni and Babesia sp. Xinjiang isolate. Analytic sensitivity results revealed that real-time PCR could detect as few as 10 copies of the gltA gene. The performance of real-time PCR was assessed by testing 254 blood samples from goats and comparing with the results from conventional PCR. This demonstrated that the real-time PCR assay was significantly more sensitive than conventional PCR. Our results indicated that real-time PCR is a useful approach for detecting A. ovis infections and has potential as an alternative tool for ecological and epidemiological surveillance of ovine anaplasmosis.
[Mh] Termos MeSH primário: Anaplasma ovis/genética
Anaplasmose/diagnóstico
DNA Bacteriano/análise
Reação em Cadeia da Polimerase em Tempo Real/métodos
Ruminantes/microbiologia
[Mh] Termos MeSH secundário: Anaplasma/genética
Anaplasma marginale/genética
Anaplasma phagocytophilum
Anaplasmose/microbiologia
Animais
Cabras/microbiologia
Dados de Sequência Molecular
Curva ROC
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Reprodutibilidade dos Testes
Ovinos/microbiologia
Doenças dos Ovinos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:160511
[Lr] Data última revisão:
160511
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140305
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12139


  6 / 25 MEDLINE  
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[PMID]:24589109
[Au] Autor:Renneker S; Abdo J; Salih DE; Karagenç T; Bilgiç H; Torina A; Oliva AG; Campos J; Kullmann B; Ahmed J; Seitzer U
[Ad] Endereço:Division of Veterinary Infection Biology and Immunology, Research Center Borstel, Borstel, Germany.
[Ti] Título:Can Anaplasma ovis in small ruminants be neglected any longer?
[So] Source:Transbound Emerg Dis;60 Suppl 2:105-12, 2013 Nov.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Anaplasma species are obligate intracellular rickettsial pathogens transmitted by ticks with an impact on human and animal health. Anaplasma ovis infects sheep and goats in many regions of the world, and it can be diagnosed by different methods like Giemsa staining, PCR or competitive ELISA. In this study, a PCR based on the gene coding for major surface protein 4 (MSP-4) was used to examine field samples collected from sheep in different countries. Altogether, 1161 blood samples from Turkey (n = 830), Iraq (n = 195), Sudan (n = 96) and Portugal (n = 40) were examined, of which 31.4%, 66.6% 41.6% and 82.5%, respectively, were positive. This indicates high prevalence of A. ovis in the countries under investigation, and it can be assumed that the situation in other areas of the world might be similar. Thus, A. ovis should be considered as an important constraint of livestock production, and further efforts are needed to better understand the epidemiology and to implement suitable control measures.
[Mh] Termos MeSH primário: Anaplasma ovis/isolamento & purificação
Anaplasma/isolamento & purificação
Surtos de Doenças
Doenças Negligenciadas/epidemiologia
Ruminantes/microbiologia
[Mh] Termos MeSH secundário: Anaplasma/genética
Anaplasma ovis/genética
Anaplasma ovis/imunologia
Animais
Anticorpos Antibacterianos/imunologia
DNA Bacteriano/genética
Ensaio de Imunoadsorção Enzimática/métodos
Cabras/microbiologia
Seres Humanos
Doenças Negligenciadas/microbiologia
Reação em Cadeia da Polimerase
Portugal/epidemiologia
Ovinos/microbiologia
Doenças dos Ovinos/epidemiologia
Doenças dos Ovinos/microbiologia
Doenças dos Ovinos/transmissão
Doenças Transmitidas por Carrapatos/microbiologia
Doenças Transmitidas por Carrapatos/transmissão
Carrapatos/microbiologia
Turquia/epidemiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (DNA, Bacterial)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:160511
[Lr] Data última revisão:
160511
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140305
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12149


  7 / 25 MEDLINE  
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[PMID]:24053615
[Au] Autor:Ciani E; Alloggio I; Petazzi F; Pieragostini E
[Ad] Endereço:Dipartimento dell'Emergenza e dei Trapianti di Organi - Unità di Cliniche Veterinarie e Produzioni Animali, Università degli Studi di Bari, Via Amendola 165/A, 70126 Bari, Italy. elisa.pieragostini@uniba.it.
[Ti] Título:Looking for prognosticators in ovine anaplasmosis: discriminant analysis of clinical and haematological parameters in lambs belonging to differently susceptible breeds experimentally infected with Anaplasma ovis.
[So] Source:Acta Vet Scand;55:71, 2013 Sep 22.
[Is] ISSN:1751-0147
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A study was carried out to evaluate the response of different native sheep breeds to experimental infection with Anaplasma ovis, the most prevalent sheep tick-borne pathogen in Apulia (Southern Italy). Thirty-four lambs belonging to a Northern European breed (Suffolk) and two Southern Italian breeds (Comisana and Altamurana) were infected. Eleven clinical as well as haematological parameters were monitored at different temporal resolutions on the same subjects before and after the infection, resulting in a data set of 435 observations. The present work, aiming to further the research, presents the results of a multivariate analysis carried out to identify which parameters out of the eleven considered are the most reliable parameters to be considered as markers of the disease phenotype as well as prognosticators of practical clinical importance. RESULTS: Data were analysed by discriminant analysis. Out of the eleven considered variables (red blood cells, packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin content, haemoglobin concentration, white blood cells, neutrophils, leukocytes, platelets, rectal temperature), only seven were included in the step-wise model since significantly increasing the Mahlanobis distance between the two closest groups. Both discriminant functions resulted to be highly significant (P < 0.0001) and the percentage of variation accounted for by the first discriminant function was 63.6% of the variance in the grouping variable. CONCLUSIONS: Taken together, the observed results stress the marked differentiation among the three breeds in terms of physio-pathological phenotypes indicating packed cell volume and red blood cell count as the most informative parameters in the routine clinical practice for A. ovis infection in sheep.
[Mh] Termos MeSH primário: Anaplasma ovis
Anaplasmose/patologia
Predisposição Genética para Doença
Doenças dos Ovinos/genética
[Mh] Termos MeSH secundário: Anaplasmose/sangue
Animais
Análise Discriminante
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1401
[Cu] Atualização por classe:150422
[Lr] Data última revisão:
150422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130924
[St] Status:MEDLINE
[do] DOI:10.1186/1751-0147-55-71


  8 / 25 MEDLINE  
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[PMID]:23182548
[Au] Autor:Torina A; Agnone A; Blanda V; Alongi A; D'Agostino R; Caracappa S; Marino AM; Di Marco V; de la Fuente J
[Ad] Endereço:Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi, n. 3, 90123 Palermo, Italy. alessandra.torina@izssicilia.it
[Ti] Título:Development and validation of two PCR tests for the detection of and differentiation between Anaplasma ovis and Anaplasma marginale.
[So] Source:Ticks Tick Borne Dis;3(5-6):283-7, 2012 Dec.
[Is] ISSN:1877-9603
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Anaplasma ovis and Anaplasma marginale are tick-transmitted bacteria that cause anaplasmosis in domestic and wild animals. Recent results show that some domestic and wild animals and ticks are susceptible to both A. ovis and A. marginale, thus supporting the need to differentiate between these species in hosts and ticks diagnosed with Anaplasma infection. However, although anaplasmosis is one of the most common diseases of grazing animals worldwide, rapid and effective tests are not available for the detection of and discrimination between these 2 Anaplasma species. The objective of this research was to develop an easy and reliable method to identify and discriminate between the closely related pathogens A. ovis and A. marginale. A. ovis and A. marginale major surface protein 4 (msp4) gene sequences were retrieved from different geographic strains and aligned to design 2 sets of primers in a region with significant differences between the 2 species, but completely conserved among strains. PCR reactions using these primers were 100% species-specific and detected all strains from each pathogen previously identified with other methods. The 2 sets of primers designed for the specific PCR amplification of A. ovis and A. marginale allow easy-to-detect and discriminate between the 2 pathogens, thus avoiding the time-consuming sequencing or multi-gene amplification procedures. This PCR provides a tool for the detection of A. ovis and A. marginale in ticks and in wildlife and domestic hosts.
[Mh] Termos MeSH primário: Anaplasma marginale/isolamento & purificação
Anaplasma ovis/isolamento & purificação
Anaplasmose/microbiologia
Técnicas Bacteriológicas/métodos
Técnicas de Diagnóstico Molecular/métodos
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Anaplasma marginale/genética
Anaplasma ovis/genética
Anaplasmose/diagnóstico
Animais
Proteínas de Bactérias/genética
Primers do DNA/genética
DNA Bacteriano/química
DNA Bacteriano/genética
Dados de Sequência Molecular
Sensibilidade e Especificidade
Análise de Sequência de DNA
Medicina Veterinária/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA Primers); 0 (DNA, Bacterial)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:121204
[Lr] Data última revisão:
121204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121128
[St] Status:MEDLINE


  9 / 25 MEDLINE  
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[PMID]:21094505
[Au] Autor:Hornok S; Micsutka A; Fernández de Mera IG; Meli ML; Gönczi E; Tánczos B; Mangold AJ; Farkas R; Lutz H; Hofmann-Lehmann R; de la Fuente J
[Ad] Endereço:Department of Parasitology and Zoology, Szent István University, Budapest, Hungary. Hornok.Sandor@aotk.szie.hu
[Ti] Título:Fatal bovine anaplasmosis in a herd with new genotypes of Anaplasma marginale, Anaplasma ovis and concurrent haemoplasmosis.
[So] Source:Res Vet Sci;92(1):30-5, 2012 Feb.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Haematological and molecular analysis of blood samples was carried out during an outbreak of bovine anaplasmosis in Hungary. Acute disease was observed in five animals, two of which died. Anaplasma-carrier state was diagnosed in 69 (92%) of cattle. Further evaluation of 24 blood samples revealed concurrent infections with Mycoplasma wenyonii and 'CandidatusM. haemobos' in 22 and 21 animals, respectively. In addition, two cows were identified with rickettsaemia. Regarding molecular investigation of potential hard tick vectors, Haemaphysalis inermis and Dermacentor marginatus males collected from the animals were PCR-negative. However, in one pool (out of 18) of Ixodesricinus males, and in six pools (out of 18) of D. reticulatus males the msp4 gene of Anaplasma marginale was detected. In the same I. ricinus pool Anaplasma ovis was also identified. All ticks were negative for haemoplasmas. Anaplasma sequences yielded 97-99% homology to sequences deposited in the Genbank. This is the first report of fatal bovine anaplasmosis associated with divergent A. marginale genotypes and concurrent 'CandidatusM. haemobos' infection, as well as of an A. ovis strain in ticks collected from cattle.
[Mh] Termos MeSH primário: Anaplasma marginale/genética
Anaplasma ovis/genética
Anaplasmose/microbiologia
Doenças dos Bovinos/microbiologia
Coinfecção/veterinária
Surtos de Doenças/veterinária
Genótipo
[Mh] Termos MeSH secundário: Anaplasma marginale/isolamento & purificação
Anaplasma ovis/isolamento & purificação
Anaplasmose/epidemiologia
Anaplasmose/transmissão
Animais
Vetores Aracnídeos/microbiologia
Proteínas de Bactérias/genética
Sequência de Bases
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/transmissão
Coinfecção/epidemiologia
Coinfecção/microbiologia
DNA Bacteriano/análise
DNA Bacteriano/genética
Dermacentor/microbiologia
Hungria/epidemiologia
Ixodes/microbiologia
Masculino
Proteínas de Membrana/genética
Dados de Sequência Molecular
Mycoplasma/genética
Mycoplasma/isolamento & purificação
Infecções por Mycoplasma/microbiologia
Infecções por Mycoplasma/veterinária
Filogenia
Reação em Cadeia da Polimerase/veterinária
Prevalência
Rickettsia/genética
Rickettsia/isolamento & purificação
Infecções por Rickettsia/microbiologia
Infecções por Rickettsia/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Membrane Proteins); 0 (Msp4 protein, Anaplasma marginale)
[Em] Mês de entrada:1208
[Cu] Atualização por classe:111221
[Lr] Data última revisão:
111221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101125
[St] Status:MEDLINE
[do] DOI:10.1016/j.rvsc.2010.10.011


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Texto completo
[PMID]:21923269
[Au] Autor:Hornok S; de la Fuente J; Biró N; Fernández de Mera IG; Meli ML; Elek V; Gönczi E; Meili T; Tánczos B; Farkas R; Lutz H; Hofmann-Lehmann R
[Ad] Endereço:Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University, Budapest, Hungary.
[Ti] Título:First molecular evidence of Anaplasma ovis and Rickettsia spp. in keds (Diptera: Hippoboscidae) of sheep and wild ruminants.
[So] Source:Vector Borne Zoonotic Dis;11(10):1319-21, 2011 Oct.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To evaluate the presence of rickettsial agents in hippoboscid flies with molecular methods, 81 sheep keds (Melophagus ovinus) were collected from 23 sheep, 144 deer keds (Lipoptena cervi) were caught in the environment, and a further 463 and 59 individuals of the latter species were obtained from fresh carcasses of 29 red deer and 17 roe deer, respectively. DNA was extracted individually or in pools. Anaplasma ovis was demonstrated in all examined sheep keds, and from one pool of free-living deer keds. Rickettsia helvetica or other, unidentified rickettsiae were also present in one pool of sheep keds, and in four pools of deer keds from both red deer and roe deer. This is the first account of polymerase chain reaction positivity of hippoboscid flies for A. ovis and rickettsiae. These results raise the possibility that-apart from cattle and roe deer as already reported-sheep and red deer might also play a reservoir role in the epidemiology of rickettsioses.
[Mh] Termos MeSH primário: Anaplasma ovis/genética
Anaplasmose/epidemiologia
Cervos
Dípteros/microbiologia
Infecções por Rickettsia/veterinária
Doenças dos Ovinos/epidemiologia
[Mh] Termos MeSH secundário: Anaplasma ovis/isolamento & purificação
Anaplasmose/transmissão
Animais
Animais Selvagens
Sequência de Bases
DNA Bacteriano/genética
Cervos/microbiologia
Cervos/parasitologia
Reservatórios de Doenças
Ectoparasitoses/parasitologia
Ectoparasitoses/veterinária
Seres Humanos
Hungria/epidemiologia
Insetos Vetores/microbiologia
Dados de Sequência Molecular
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Rickettsia/genética
Rickettsia/isolamento & purificação
Infecções por Rickettsia/epidemiologia
Infecções por Rickettsia/transmissão
Ovinos
Doenças dos Ovinos/microbiologia
Doenças dos Ovinos/parasitologia
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:111006
[Lr] Data última revisão:
111006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110920
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2011.0649



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