Base de dados : MEDLINE
Pesquisa : B03.440.050 [Categoria DeCS]
Referências encontradas : 322 [refinar]
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[PMID]:28966273
[Au] Autor:El-Moselhy TF; Sidhom PA; Esmat EA; El-Mahdy NA
[Ad] Endereço:Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Tanta University.
[Ti] Título:Synthesis, Docking Simulation, Biological Evaluations and 3D-QSAR Study of 1,4-Dihydropyridines as Calcium Channel Blockers.
[So] Source:Chem Pharm Bull (Tokyo);65(10):893-903, 2017.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Resurgence to target L-type voltage-dependent calcium channels has been applied by the synthesis of two series of nifedipine analogues where the ortho- or a meta-nitrophenyl ring is retained. A pre-synthetic molecular docking study with a receptor model followed by molecular alignment has been performed on 47 compounds to predict the most active member. The IC values revealed that some of the compounds are similar to or more active than nifedipine. Substitution of groups at the 3- and 5-positions of the dihydropyridine (DHP) ring gave 3k, which is more active than nifedipine. Our valid three-dimensional quantitative structure-activity relationship (3D-QSAR) model prefigures the influence of lipophilicity, bulkiness and chelating effects of the C3 and C5 substituents. Bulky groups interfere with ring-to-ring hydrophobic interaction with tyrosine (Tyr) and limit the efficiency of increasing the length of the hydrocarbon chain of esters at the 3- and 5-positions of the DHP ring as an approach to increasing the activity. The presence of a chelating substituent on the phenyl ring at the 4-position of the DHP ring ensures strong binding to the receptor and hence stabilization of the closed-channel conformation. The validation of 3D-QSAR model indicated its proficiency in predicting activity of newly compounds belonging to the same chemical class.
[Mh] Termos MeSH primário: Bloqueadores dos Canais de Cálcio/síntese química
Di-Hidropiridinas/química
Di-Hidropiridinas/síntese química
[Mh] Termos MeSH secundário: Animais
Arcobacter/metabolismo
Proteínas de Bactérias/metabolismo
Sítios de Ligação
Bloqueadores dos Canais de Cálcio/química
Bloqueadores dos Canais de Cálcio/metabolismo
Canais de Cálcio Tipo L/química
Canais de Cálcio Tipo L/metabolismo
Di-Hidropiridinas/metabolismo
Concentração Inibidora 50
Simulação de Acoplamento Molecular
Estrutura Terciária de Proteína
Relação Quantitativa Estrutura-Atividade
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Calcium Channel Blockers); 0 (Calcium Channels, L-Type); 0 (Dihydropyridines); 0 (L-type calcium channel alpha(1C)); 7M8K3P6I89 (1,4-dihydropyridine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00186


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[PMID]:28906158
[Au] Autor:Jribi H; Sellami H; Hassena AB; Gdoura R
[Ad] Endereço:1 Laboratoire de recherche Toxicologie Microbiologie Environnementale et Santé (LR17ES06), Faculté des Sciences de Sfax, Université de Sfax, Sfax 3038, Tunisia.
[Ti] Título:Prevalence of Putative Virulence Genes in Campylobacter and Arcobacter Species Isolated from Poultry and Poultry By-Products in Tunisia.
[So] Source:J Food Prot;80(10):1705-1710, 2017 Oct.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Campylobacter and Arcobacter spp. are common causes of gastroenteritis in humans; these infections are commonly due to undercooked poultry. However, their virulence mechanism is still poorly understood. The aim of this study was to evaluate the presence of genotypic virulence markers in Campylobacter and Arcobacter species using PCR. The prevalence of virulence and cytolethal distending toxin (CDT) genes was estimated in 71 Campylobacteraceae isolates. PCR was used to detect the presence of virulence genes (iam, cadF, virB1, flaA, cdtA, cdtB, and cdtC) using specific primers for a total of 45 Campylobacter isolates, including 37 C. jejuni and 8 C. coli. All the Campylobacter isolates were positive for the cadF gene. The plasmid gene virB11 was not detected in any strain. The invasion associated marker was not detected in C. jejuni. Lower detection rates were observed for flaA, cdtA, cdtB, and cdtC. The presence of nine putative Arcobacter virulence genes (cadF, ciaB, cj1349, mviN, pldA, tlyA, irgA, hecA, and hecB) was checked in a set of 22 Arcobacter butzleri and 4 Arcobacter cryaerophilus isolates. The pldA and mviN genes were predominant (88.64%). Lower detection rates were observed for tlyA (84.76%), ciaB (84.61%), cadF and cj1349 (76.92%), IrgA and hecA (61.53%), and hecB (57.69%). The findings revealed that a majority of the Campylobacteraceae strains have these putative virulence genes that may lead to pathogenic effects in humans.
[Mh] Termos MeSH primário: Arcobacter/patogenicidade
Campylobacter/patogenicidade
Virulência/genética
[Mh] Termos MeSH secundário: Animais
Arcobacter/genética
Campylobacter/genética
Campylobacter jejuni
Seres Humanos
Reação em Cadeia da Polimerase
Aves Domésticas
Prevalência
Tunísia
Fatores de Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Virulence Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-509


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[PMID]:28820118
[Au] Autor:Tanaka R; Cleenwerck I; Mizutani Y; Iehata S; Bossier P; Vandamme P
[Ad] Endereço:1​Laboratory of Marine Microbiology, Mie University, Kurima-machiya 1577, Tsu, Mie, 514-8507, Japan.
[Ti] Título:Arcobacter haliotis sp. nov., isolated from abalone species Haliotis gigantea.
[So] Source:Int J Syst Evol Microbiol;67(8):3050-3056, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-negative, aerobic, polar-flagellated and rod-shaped, sometimes slightly curved bacterium, designated MA5T, was isolated from the gut of an abalone of the species Haliotis gigantea collected in Japan. Phylogenetic analyses based on 16S rRNA, gyrB, hsp60 and rpoB gene sequences placed strain MA5T in the genus Arcobacter in an independent phylogenetic line. Comparison of the 16S rRNA gene sequence of this strain with those of the type strains of the established Arcobacter species revealed A. nitrofigilis (95.1 %) as nearest neighbour. Strain MA5T grew optimally at 25 °C, pH 6.0 to 9.0 and in the presence of 2 to 5 % (w/v) NaCl under both aerobic and microaerobic conditions. The predominant fatty acids found were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c), C12 : 0 3-OH and C18 : 1 ω7c. Menaquinone-6 (MK-6) and menaquinone-7 (MK-7) were found as the major respiratory quinones. The major polar lipids detected were phosphatidylethanolamine and phosphatidylglycerol. Strain MA5T could be differentiated phenotypically from the phylogenetic closest Arcobacter species by its ability to grow on 0.05 % safranin and 0.01 % 2,3,5-triphenyl tetrazolium chloride (TTC), but not on 0.5 % NaCl. The obtained DNA G+C content of strain MA5T was 27.9 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic distinctiveness of MA5T, this strain is considered to represent a novel species of the genus Arcobacter, for which the name Arcobacter haliotis sp. nov. is proposed. The type strain is MA5T (=LMG 28652T=JCM 31147T).
[Mh] Termos MeSH primário: Arcobacter/classificação
Gastrópodes/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Arcobacter/genética
Arcobacter/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Genes Bacterianos
Japão
Fosfatidiletanolaminas/química
Fosfatidilgliceróis/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylethanolamines); 0 (Phosphatidylglycerols); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 39382-08-6 (phosphatidylethanolamine); 71ANL51TLA (menaquinone 6); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002080


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[PMID]:28671965
[Au] Autor:Rovetto F; Carlier A; Van den Abeele AM; Illeghems K; Van Nieuwerburgh F; Cocolin L; Houf K
[Ad] Endereço:Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, Belgium.
[Ti] Título:Characterization of the emerging zoonotic pathogen Arcobacter thereius by whole genome sequencing and comparative genomics.
[So] Source:PLoS One;12(7):e0180493, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Four Arcobacter species have been associated with human disease, and based on current knowledge, these Gram negative bacteria are considered as potential food and waterborne zoonotic pathogens. At present, only the genome of the species Arcobacter butzleri has been analysed, and still little is known about their physiology and genetics. The species Arcobacter thereius has first been isolated from tissue of aborted piglets, duck and pig faeces, and recently from stool of human patients with enteritis. In the present study, the complete genome and analysis of the A. thereius type strain LMG24486T, as well as the comparative genome analysis with 8 other A. thereius strains are presented. Genome analysis revealed metabolic pathways for the utilization of amino acids, which represent the main source of energy, together with the presence of genes encoding for respiration-associated and chemotaxis proteins. Comparative genome analysis with the A. butzleri type strain RM4018 revealed a large correlation, though also unique features. Furthermore, in silico DDH and ANI based analysis of the nine A. thereius strains disclosed clustering into two closely related genotypes. No discriminatory differences in genome content nor phenotypic behaviour were detected, though recently the species Arcobacter porcinus was proposed to encompass part of the formerly identified Arcobacter thereius strains. The report of the presence of virulence associated genes in A. thereius, the presence of antibiotic resistance genes, verified by in vitro susceptibility testing, as well as other pathogenic related relevant features, support the classification of A. thereius as an emerging pathogen.
[Mh] Termos MeSH primário: Arcobacter/genética
Genoma Bacteriano
Sequenciamento de Nucleotídeos em Larga Escala/métodos
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Arcobacter/classificação
Arcobacter/efeitos dos fármacos
Patos/microbiologia
Seres Humanos
Testes de Sensibilidade Microbiana
Filogenia
Suínos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180493


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[PMID]:28598346
[Au] Autor:Banihashemi A; Van Dyke MI; Huck PM
[Ad] Endereço:NSERC Chair in Water Treatment, Department of Civil and Environmental Engineering, University of Waterloo, 200 University Avenue West, Waterloo, ON, Canada N2L 3G1 E-mail: mvandyke@uwaterloo.ca.
[Ti] Título:Application of long amplicon propidium monoazide-PCR to assess the effects of temperature and background microbiota on pathogens in river water.
[So] Source:J Water Health;15(3):418-428, 2017 Jun.
[Is] ISSN:1477-8920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The decay rates of enteric waterborne pathogens were evaluated following the introduction of Yersinia enterocolitica, Salmonella enterica, Campylobacter jejuni and Arcobacter butzleri into river water at different temperatures (5, 15 and 25°C) for a period of 28 days. To improve the accuracy of the results a molecular viability assay, long amplicon propidium monoazide-polymerase chain reaction (PMA-PCR), was used to quantify the viable cell concentration and results from PCR with and without PMA were compared. As well, the effect of background microbiota was assessed for Y. enterocolitica and S. enterica by inoculating cells into sterile and non-sterile river water. Cell persistence was improved by up to 4 log for Y. enterocolitica and 4.5 log for S. enterica in sterile river water compared to natural river water, showing that the autochthonous biological activity in river water can accelerate the die-off of introduced bacteria. Results also showed that low temperature significantly improved the persistence of all four target bacteria in non-sterile river water. There was a more rapid decline in cell concentration in samples with PMA pretreatment; therefore using PMA-PCR analysis can provide more reliable data on viable/active enteric bacteria in aquatic microcosms and allows for improved assessment of pathogens in the environment.
[Mh] Termos MeSH primário: Azidas/química
Bactérias/isolamento & purificação
Microbiota/fisiologia
Reação em Cadeia da Polimerase
Propídio/análogos & derivados
Rios/microbiologia
Temperatura Ambiente
[Mh] Termos MeSH secundário: Arcobacter/isolamento & purificação
Campylobacter jejuni/isolamento & purificação
Viabilidade Microbiana
Ontário
Propídio/química
Salmonella enterica/isolamento & purificação
Yersinia enterocolitica/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azides); 0 (propidium monoazide); 36015-30-2 (Propidium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.2166/wh.2017.161


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[PMID]:28400014
[Au] Autor:González A; Bayas Morejón IF; Ferrús MA
[Ad] Endereço:Department of Biotechnology, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
[Ti] Título:Isolation, molecular identification and quinolone-susceptibility testing of Arcobacter spp. isolated from fresh vegetables in Spain.
[So] Source:Food Microbiol;65:279-283, 2017 Aug.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Some species of the Arcobacter genus are considered emerging foodborne and waterborne enteropathogens. However, the presence of Arcobacter spp. in vegetables very little is known, because most studies have focused on foods of animal origin. On the other hand, quinolones are considered as first-line drugs for the treatment of infection by campylobacteria in human patients, but few data are currently available about the resistance levels to these antibiotics among Arcobacter species. Therefore, the aim of this study was to investigate the presence and diversity of arcobacters isolated from fresh vegetables such as lettuces, spinaches, chards and cabbages. Resistance to quinolones of the isolates was also investigated. One hundred fresh vegetables samples purchased from seven local retail markets in Valencia (Spain) during eight months were analysed. The study included 41 lettuces, 21 spinaches, 34 chards and 4 cabbages. Samples were analysed by culture and by molecular methods before and after enrichment. By culture, 17 out of 100 analysed samples were Arcobacter positive and twenty-five isolates were obtained from them. Direct detection by PCR was low, with only 4% Arcobacter spp. positive samples. This percentage increased considerably, up 20%, after 48 h enrichment. By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), 17 out of the 25 isolates were identified as A. butzleri and 8 as A. cryaerophilus. Only two A. butzleri isolates showed resistance to levofloxacin and ciprofloxacin. The sequencing of a fragment of the QRDR region of the gyrA gene from the quinolones-resistant isolates revealed the presence of a mutation in position 254 of this gene (C-T transition). This study is the first report about the presence of pathogenic species of Arcobacter spp. in chards and cabbages and confirms that fresh vegetables can act as transmission vehicle to humans. Moreover, the presence of A. butzleri quinolone resistant in vegetables could pose a potential public health risk.
[Mh] Termos MeSH primário: Arcobacter/genética
Arcobacter/isolamento & purificação
Quinolonas/farmacologia
Verduras/microbiologia
[Mh] Termos MeSH secundário: Arcobacter/efeitos dos fármacos
Arcobacter/patogenicidade
DNA Girase/genética
Farmacorresistência Bacteriana
Microbiologia de Alimentos
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/prevenção & controle
Infecções por Bactérias Gram-Negativas/transmissão
Seres Humanos
Alface/microbiologia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Espanha
Spinacia oleracea/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Quinolones); EC 5.99.1.3 (DNA Gyrase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170427
[Lr] Data última revisão:
170427
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE


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[PMID]:28394467
[Au] Autor:Rathlavath S; Kumar S; Nayak BB
[Ad] Endereço:Quality Control Laboratory, Post-Harvest Technology Department, ICAR-Central Institute of Fisheries Education (CIFE), Mumbai, India.
[Ti] Título:Comparative isolation and genetic diversity of Arcobacter sp. from fish and the coastal environment.
[So] Source:Lett Appl Microbiol;65(1):42-49, 2017 Jul.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arcobacter species are emerging food-borne and water-borne human pathogens associated mostly with food animals and their environment. The present study was aimed to isolate Arcobacter species from fish, shellfish and coastal water samples using two methods and to determine their genetic diversity. Of 201 samples of fish, shellfish and water samples analysed, 66 (32·8%) samples showed the presence of Arcobacter DNA from both Arcobacter enrichment broth and Bolton broth. Arcobacters were isolated from 58 (87·8%) and 38 (57·5%) of Arcobacter DNA-positive samples using Arcobacter blood agar and Preston blood agar, respectively. Arcobacter sp. identified by biochemical tests were further analysed by a genus-specific PCR, followed by a multiplex-PCR and 16S rRNA-RFLP. From both the methods, four different Arcobacter species namely Arcobacter butzleri, Arcobacter skirrowii, Arcobacter mytili and Arcobacter defluvii were isolated, of which A. butzleri was the predominant species. Enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprint analysis revealed that the arcobacters isolated in this study were genetically very diverse and no specific genotype was found associated with a specific source (seafood or water). Since pathogenic arcobacters are not known to be natural inhabitants of coastal marine environment, identifying the sources of contamination will be crucial for effective management of this problem. SIGNIFICANCE AND IMPACT OF THE STUDY: Arcobacter sp. are emerging food- and water-borne human pathogens. In this study, comparison of two selective media suggested Arcobacter blood agar to be more efficient in yielding Arcobacter sp. from seafood. Furthermore, the isolation of Arcobacter sp. such as Arcobacter butzleri, A. skirrowii, A. mytili and A. defluvii from seafood suggests diverse sources of contamination of seafood by Arcobacter sp. Analysis of enterobacterial repetitive intergenic consensus sequence-PCR patterns of A. butzleri showed high genetic diversity and lack of clonality among the isolates. Arcobacter contamination of seafood is an emerging issue both from seafood safety and seafood trade point of view.
[Mh] Termos MeSH primário: Arcobacter/genética
Arcobacter/isolamento & purificação
Peixes/microbiologia
Alimentos Marinhos/microbiologia
Frutos do Mar/microbiologia
[Mh] Termos MeSH secundário: Animais
Arcobacter/classificação
DNA Bacteriano/genética
Variação Genética
Genótipo
Seres Humanos
Reação em Cadeia da Polimerase Multiplex
Reação em Cadeia da Polimerase/métodos
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12743


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[PMID]:28321791
[Au] Autor:Oliveira MG; Pressinotti LN; Carvalho GS; Oliveira MC; Moreno LZ; Matajira CE; Bergamo AS; Aleixo VM; Veiga AC; de Souza Corsino E; Christ AP; Sato MI; Moreno AM; Knöbl T
[Ad] Endereço:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87-Cidade Universitária, São Paulo, SP, Brazil.
[Ti] Título:Arcobacter spp. in fecal samples from Brazilian farmed caimans (Caiman yacare, Daudin 1802).
[So] Source:Trop Anim Health Prod;49(4):777-782, 2017 Apr.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans.
[Mh] Termos MeSH primário: Jacarés e Crocodilos/microbiologia
Arcobacter/isolamento & purificação
[Mh] Termos MeSH secundário: Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Animais
Brasil
Fezes/microbiologia
Variação Genética
Reação em Cadeia da Polimerase
Virulência/genética
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Virulence Factors)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1262-3


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[PMID]:28177789
[Au] Autor:Webb AL; Taboada EN; Selinger LB; Boras VF; Inglis GD
[Ad] Endereço:a Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada.
[Ti] Título:Prevalence and diversity of waterborne Arcobacter butzleri in southwestern Alberta, Canada.
[So] Source:Can J Microbiol;63(4):330-340, 2017 Apr.
[Is] ISSN:1480-3275
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Arcobacter butzleri is a potential enteric pathogen to human beings, but its reservoirs and modes of transmission are largely unverified. Microbiological and molecular detection and subtyping techniques can facilitate surveillance of A. butzleri in hosts and environmental reservoirs. We isolated A. butzleri from 173 surface water samples (25.6%) and 81 treated wastewater samples (77.9%) collected in southwestern Alberta over a 1-year period. Arcobacter butzleri isolates (n = 500) were genotyped and compared to determine diversity of A. butzleri in southwestern Alberta. Culture methods affected the frequency of detection and genotype diversity of A. butzleri, and isolation comprehensiveness was different for surface waters and treated wastewaters. Detection of A. butzleri in the Oldman River Watershed corresponded with season, river flow rates, and fecal coliform densities. Arcobacter butzleri was detected most frequently in treated wastewater, in the Oldman River downstream from treated wastewater outfalls, and in tributaries near areas of intensive confined feeding operations. All sample sources possessed high genotype diversity, and A. butzleri isolates from treated wastewaters were genetically similar to isolates from the Oldman River downriver from treated wastewater outfall sites. In southwestern Alberta, municipal and agricultural activities contribute to the density and genotype diversity of A. butzleri in surface waters.
[Mh] Termos MeSH primário: Arcobacter/isolamento & purificação
Rios/microbiologia
Águas Residuais/microbiologia
[Mh] Termos MeSH secundário: Alberta
Animais
Arcobacter/classificação
Arcobacter/genética
Fezes/microbiologia
Genótipo
Seres Humanos
Prevalência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1139/cjm-2016-0745


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[PMID]:28121468
[Au] Autor:Morejón IF; González A; Ferrús MA
[Ad] Endereço:Department of Biotechnology, Universitat Politècnica de València , Valencia, Spain .
[Ti] Título:Detection, Identification, and Antimicrobial Susceptibility of Arcobacter spp. Isolated from Shellfish in Spain.
[So] Source:Foodborne Pathog Dis;14(4):238-243, 2017 Apr.
[Is] ISSN:1556-7125
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This work aimed to determine the presence of Arcobacter spp. in shellfish and to determine its susceptibility to quinolones. One hundred samples (41 mussels, 37 clams, and 22 cockles) were purchased from different local retail shops in Valencia, Spain, from September 2013 to June 2015. All samples were analyzed simultaneously by culture, after an enrichment step, and by polymerase chain reaction (PCR), directly and after enrichment. The susceptibility to levofloxacin and ciprofloxacin of the isolates was tested using the disk-diffusion test and E-test strips method. To clarify the mechanism of quinolone resistance, a fragment of the quinolone resistance-determining region of the gyrA gene was sequenced. Thirty-seven samples were positive and 49 isolates were obtained by culture, and Arcobacter spp. DNA was detected in 32% of the samples by PCR. However, after 48-h enrichment, the number of positive samples increased, and 68 of the 100 samples yielded the specific Arcobacter spp. PCR product. In addition, 49 isolates were identified by PCR-restriction fragment length polymorphism. The most commonly found species was Arcobacter butzleri (25 isolates, 51.03%) followed by Arcobacter cryaerophilus (19 isolates, 38.77%) and Arcobacter defluvii (5 isolates, 10.20%). Only three isolates of A. butzleri were resistant to both antibiotics. A mutation C to T transition in the position 254 of the gyrA gene was present in the three resistant isolates. This study confirms that pathogenic arcobacters are frequently found in edible shellfish samples. Moreover, this is the first time that A. butzleri and A. cryaerophilus have been isolated from cockles.
[Mh] Termos MeSH primário: Arcobacter/efeitos dos fármacos
Arcobacter/genética
Proteínas de Bactérias/genética
Farmacorresistência Bacteriana Múltipla
Frutos do Mar/microbiologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Arcobacter/isolamento & purificação
Proteínas de Bactérias/metabolismo
DNA Bacteriano/isolamento & purificação
Contaminação de Alimentos/análise
Microbiologia de Alimentos
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Quinolonas/farmacologia
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Quinolones)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.1089/fpd.2016.2202



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