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[PMID]:28369531
[Au] Autor:Costa MO; Fernando C; Nosach R; Harding JCS; Hill JE
[Ad] Endereço:Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, SK S7N 5B4, Canada.
[Ti] Título:Infection of porcine colon explants with "Brachyspira hampsonii" leads to increased epithelial necrosis and catarrhal exudate.
[So] Source:Pathog Dis;75(3), 2017 Apr 01.
[Is] ISSN:2049-632X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mucohemorrhagic diarrhea in pigs caused by Brachyspira spp. has a global distribution, and an economic impact on affected farms due to poor performance of animals. Demonstrations that "Brachyspira hampsonii" is pathogenic have been achieved using in vivo animal models, but a critical knowledge gap exists regarding the pathogenic mechanisms employed by Brachyspira. Here, we used in vitro organ culture of porcine colon to investigate interactions between "B. hampsonii" and explants during the first 12 h of contact. Explants were either inoculated with "B. hampsonii" or sterile culture broth. Responses to infection were evaluated by optical microscopy and quantitative PCR. Significantly greater numbers of necrotic crypt cells and thicker catarrhal exudate were observed on infected explants compared to controls. Spirochaetes were observed in the mucus layer, in contact with necrotic exfoliated cells, in crypts and the lamina propria. Statistical differences were observed in mRNA levels between inoculated and control explants for IL-1α, TNF-α and ZO-1 using a Bayesian analysis, but not observed using the ΔΔCq method. These results provide a demonstration of a porcine colon explant model for investigating interactions of Brachyspira with its host and show that initial effects on the host are observed within the first 12 h of contact.
[Mh] Termos MeSH primário: Brachyspira/fisiologia
Colite/veterinária
Infecções por Bactérias Gram-Negativas/veterinária
Mucosa Intestinal/microbiologia
Mucosa Intestinal/patologia
Doenças dos Suínos/microbiologia
Doenças dos Suínos/patologia
[Mh] Termos MeSH secundário: Animais
Biomarcadores
Biópsia
Citocinas/metabolismo
Exsudatos e Transudatos
Mediadores da Inflamação/metabolismo
Mucosa Intestinal/metabolismo
Masculino
Necrose
Suínos
Doenças dos Suínos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Cytokines); 0 (Inflammation Mediators)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1093/femspd/ftx032


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[PMID]:28324657
[Au] Autor:Kulathunga DGRS; Rubin JE
[Ad] Endereço:Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
[Ti] Título:A review of the current state of antimicrobial susceptibility test methods for Brachyspira.
[So] Source:Can J Microbiol;63(6):465-474, 2017 Jun.
[Is] ISSN:1480-3275
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:The re-emergence of swine dysentery (Brachyspira-associated muco-haemorrhagic colitis) since the late 2000s has illuminated diagnostic challenges associated with this genus. The methods used to detect, identify, and characterize Brachyspira from clinical samples have not been standardized, and laboratories frequently rely heavily on in-house techniques. Particularly concerning is the lack of standardized methods for determining and interpreting the antimicrobial susceptibility of Brachyspira spp. The integration of laboratory data into a treatment plan is a critical component of prudent antimicrobial usage. Therefore, the lack of standardized methods is an important limitation to the evidence-based use of antimicrobials. This review will focus on describing the methodological limitations and inconsistencies between current susceptibility testing schemes employed for Brachyspira, provide an overview of what we do know about the susceptibility of these organisms, and suggest future directions to improve and standardize diagnostic strategies.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Brachyspira/efeitos dos fármacos
Testes de Sensibilidade Microbiana
[Mh] Termos MeSH secundário: Animais
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/veterinária
Suínos
Doenças dos Suínos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Infective Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1139/cjm-2016-0756


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[PMID]:28188751
[Au] Autor:Ogata S; Shimizu K; Tominaga S; Nakanishi K
[Ad] Endereço:Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Saitama 359-8513, Japan; Department of Diagnostic Pathology, JCHO Saitama Medical Center, Saitama, Saitama 330-0074, Japan. Electronic address: sogata@ndmc.ac.jp.
[Ti] Título:Immunohistochemical study of mucins in human intestinal spirochetosis.
[So] Source:Hum Pathol;62:126-133, 2017 Apr.
[Is] ISSN:1532-8392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Most patients with human intestinal spirochetosis (HIS; a colorectal bacterial infection caused by Brachyspira species) seem asymptomatic, and its pathogenicity remains unclear. Recently, alterations in mucin expression were reported in animal Brachyspira infection. The present question was "Is mucin expression altered in HIS?" Using antibodies for MUCs 1, 2, 4, 5AC, and 6, we immunohistochemically compared 215 specimens from 83 histology-confirmed HIS cases with 106 specimens from 26 non-HIS cases. Positive staining (which included even focal positive staining) was rated "high (+)" or "low (+)." Results were analyzed for 4 categories of lesions, and associations between MUC expression and spirochetal presence were also analyzed. In the "specimens without polyps or adenocarcinoma" category, high (+) MUC2 positivity was more frequent in HIS than in control. In the hyperplasia/serrated polyp category, in HIS (versus control), the MUC5AC positivity rate was lower, whereas high (+) MUC4 positivity was more frequent. In the conventional adenoma category, in HIS (versus control), the MUC1 positivity rate was lower, whereas both high (+) MUC2 positivity and high (+) MUC5AC positivity were less frequent. In the adenocarcinoma category, high (+) MUC2 positivity was more frequent in HIS than in control. Among the above mucins, only MUC1 positivity was significantly associated with an absence of the so-called fringe formation, an absence of spiral organisms within mucus, and an absence of strong immunopositive materials within the epithelial layer and within the subepithelial layer. The results suggest that Brachyspira infection or a related change in the microbiome may alter the large intestine mucin expression profile in humans.
[Mh] Termos MeSH primário: Adenocarcinoma/química
Pólipos Adenomatosos/química
Brachyspira/patogenicidade
Neoplasias do Colo/química
Pólipos do Colo/química
Infecções por Bactérias Gram-Negativas/metabolismo
Imuno-Histoquímica
Intestino Grosso/química
Mucinas/análise
[Mh] Termos MeSH secundário: Adenocarcinoma/microbiologia
Adenocarcinoma/patologia
Pólipos Adenomatosos/microbiologia
Pólipos Adenomatosos/patologia
Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores Tumorais/análise
Biópsia
Neoplasias do Colo/microbiologia
Neoplasias do Colo/patologia
Pólipos do Colo/microbiologia
Pólipos do Colo/patologia
Microbioma Gastrointestinal
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/patologia
Interações Hospedeiro-Patógeno
Seres Humanos
Intestino Grosso/microbiologia
Intestino Grosso/patologia
Masculino
Meia-Idade
Valor Preditivo dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Mucins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE


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[PMID]:28173799
[Au] Autor:Borgström A; Scherrer S; Kirchgässner C; Schmitt S; Frei D; Wittenbrink MM
[Ad] Endereço:Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 270, CH 8057, Zurich, Switzerland. anna.borgstroem@vetbakt.uzh.ch.
[Ti] Título:A novel multiplex qPCR targeting 23S rDNA for diagnosis of swine dysentery and porcine intestinal spirochaetosis.
[So] Source:BMC Vet Res;13(1):42, 2017 Feb 07.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A multiplex qPCR targeting a 128 bp region on the 23S rDNA gene was developed for detection of Brachyspira (B.) hyodysenteriae and B. pilosicoli, the agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), together with a triplet of apathogenic Brachyspira spp. (B. innocens, B. intermedia, B. murdochii) in porcine feces. The multiplex qPCR was evaluated against a duplex PCR (La et al., J Clin Microbiol 41:3372-5, 2003). RESULTS: Using DNA extracted from fecal culture, the multiplex qPCR showed excellent agreement with the duplex PCR (κ = 0.943 and 0.933). In addition, thanks to the three probes whereof one detecting the apathogenic Brachyspria spp., a more diversified overview of the brachyspiral flora in porcine fecal samples can be delivered as a part of the routine diagnostic. The multiplex qPCR with a limit of detection of 5-10 genomic equivalents (GE) per reaction (6 × 10 GE per gram) allows reliable detection of Brachyspira species directly from fecal swab DNA. In line with this, analysis of 202 fecal swabs in comparison with culture-based qPCR showed a high agreement for the causative agents of SD (B.hyodysenteriae: κ = 0.853, sensitivity 87% specificity 98%). CONCLUSION: The novel multiplex qPCR is robust and has a high analytical sensitivity and is therefore suitable for high-throughput screening of porcine fecal swabs for the causative agents of SD. This assay can therefore be used for the direct proof of the pathogenic B. spp. in fecal swabs within the scope of a monitoring program.
[Mh] Termos MeSH primário: Brachyspira/genética
Infecções por Bactérias Gram-Negativas/veterinária
Doenças dos Suínos/diagnóstico
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Animais
Brachyspira/isolamento & purificação
Fezes/microbiologia
Infecções por Bactérias Gram-Negativas/diagnóstico
Infecções por Bactérias Gram-Negativas/microbiologia
Limite de Detecção
Reação em Cadeia da Polimerase/veterinária
RNA Ribossômico 23S/genética
Reprodutibilidade dos Testes
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 23S)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-016-0939-6


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[PMID]:27907102
[Au] Autor:La T; Phillips ND; Hampson DJ
[Ad] Endereço:School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia.
[Ti] Título:An Investigation into the Etiological Agents of Swine Dysentery in Australian Pig Herds.
[So] Source:PLoS One;11(12):e0167424, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Swine dysentery (SD) is a mucohemorrhagic colitis, classically seen in grower/finisher pigs and caused by infection with the anaerobic intestinal spirochete Brachyspira hyodysenteriae. More recently, however, the newly described species Brachyspira hampsonii and Brachyspira suanatina have been identified as causing SD in North America and/or Europe. Furthermore, there have been occasions where strains of B. hyodysenteriae have been recovered from healthy pigs, including in multiplier herds with high health status. This study investigated whether cases of SD in Australia may be caused by the newly described species; how isolates of B. hyodysenteriae recovered from healthy herds compared to isolates from herds with disease; and how contemporary isolates compare to those recovered in previous decades, including in their plasmid gene content and antimicrobial resistance profiles. In total 1103 fecal and colon samples from pigs in 97 Australian herds were collected and tested. Of the agents of SD only B. hyodysenteriae was found, being present in 34 (35.1%) of the herds, including in 14 of 24 (58%) herds that had been considered to be free of SD. Multilocus sequence typing applied to 96 isolates from 30 herds and to 53 Australian isolates dating from the 1980s through the early 2000s showed that they were diverse, distinct from those reported in other countries, and that the 2014/16 isolates generally were different from those from earlier decades. These findings provided evidence for ongoing evolution of B. hyodysenteriae strains in Australia. In seven of the 20 herds where multiple isolates were available, two to four different sequence types (STs) were identified. Isolates with the same STs also were found in some herds with epidemiological links. Analysis of a block of six plasmid virulence-associated genes showed a lack of consistency between their presence or absence and their origin from herds currently with or without disease; however, significantly fewer isolates from the 2000s and from 2014/16 had this block of genes compared to isolates from the 1980s and 1990s. It is speculated that loss of these genes may have been responsible for the occurrence of milder disease occurring in recent years. In addition, fewer isolates from 2014/16 were susceptible to the antimicrobials lincomycin, and to a lesser extent tiamulin, than those from earlier Australian studies. Four distinct multi-drug resistant strains were identified in five herds, posing a threat to disease control.
[Mh] Termos MeSH primário: Brachyspira/patogenicidade
Disenteria/microbiologia
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Animais
Austrália
Brachyspira/genética
Brachyspira/isolamento & purificação
Disenteria/epidemiologia
Disenteria/veterinária
Fezes/microbiologia
Suínos
Doenças dos Suínos/epidemiologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170704
[Lr] Data última revisão:
170704
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0167424


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[PMID]:27664096
[Au] Autor:Aller-Morán LM; Martínez-Lobo FJ; Rubio P; Carvajal A
[Ad] Endereço:Department of Infectious Diseases and Epidemiology, Veterinary Faculty, University of León, León, Spain lallm@unileon.es.
[Ti] Título:Cross-reactions in specific Brachyspira spp. PCR assays caused by "Brachyspira hampsonii" isolates: implications for detection.
[So] Source:J Vet Diagn Invest;28(6):755-759, 2016 Nov.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An emerging novel spirochete in swine, provisionally designated "Brachyspira hampsonii," has been detected worldwide. It has been associated with swine dysentery and cannot be differentiated from B. hyodysenteriae, the classical etiologic agent of this disease, using standard phenotypic methods. We evaluated cross-reactions of "B. hampsonii" isolates recovered from avian species in some of the currently available species-specific polymerase chain reaction (PCR) assays for the identification of swine Brachyspira species. Ten avian "B. hampsonii" isolates recovered from wild waterfowl were used. No false-positive results were recorded with a B. pilosicoli-specific PCR based on the amplification of a fragment of the 16S rRNA gene. However, the percentage of false-positive results varied, with a range of 10-80%, in the evaluated B. hyodysenteriae-specific assays based on the amplification of the 23S rRNA, nox, and tlyA genes. Similarly, results of the B. intermedia-specific PCR assays yielded poor specificity, with up to 80% of the "B. hampsonii" isolates tested giving false-positive results. Finally, 2 "B. hampsonii" avian isolates yielded a positive result in a B. innocens- and B. murdochii-specific PCR. This result should be interpreted very cautiously as these 2 isolates could represent a recombinant genotype.
[Mh] Termos MeSH primário: Doenças das Aves/microbiologia
Brachyspira/isolamento & purificação
Infecções por Bactérias Gram-Negativas/veterinária
[Mh] Termos MeSH secundário: Animais
Brachyspira/classificação
Brachyspira/genética
Reações Cruzadas
Gansos
Genótipo
Infecções por Bactérias Gram-Negativas/microbiologia
Reação em Cadeia da Polimerase/veterinária
RNA Ribossômico 16S/genética
Espanha
Especificidade da Espécie
Suínos
Doenças dos Suínos/microbiologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


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[PMID]:27629903
[Au] Autor:Mirajkar NS; Phillips ND; La T; Hampson DJ; Gebhart CJ
[Ad] Endereço:Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA.
[Ti] Título:Characterization and Recognition of Brachyspira hampsonii sp. nov., a Novel Intestinal Spirochete That Is Pathogenic to Pigs.
[So] Source:J Clin Microbiol;54(12):2942-2949, 2016 Dec.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Swine dysentery (SD) is a mucohemorrhagic colitis of swine classically caused by infection with the intestinal spirochete Brachyspira hyodysenteriae Since around 2007, cases of SD have occurred in North America associated with a different strongly beta-hemolytic spirochete that has been molecularly and phenotypically characterized and provisionally named "Brachyspira hampsonii." Despite increasing international interest, B. hampsonii is currently not recognized as a valid species. To support its recognition, we sequenced the genomes of strains NSH-16 , NSH-24, and P280/1, representing B. hampsonii genetic groups I, II, and III, respectively, and compared them with genomes of other valid Brachyspira species. The draft genome of strain NSH-16 has a DNA G+C content of 27.4% and an approximate size of 3.2 Mb. Genomic indices, including digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI), and average amino acid identity (AAI), clearly differentiated B. hampsonii from other recognized Brachyspira species. Although discriminated genotypically, the three genetic groups are phenotypically similar. By electron microscopy, cells of different strains of B. hampsonii measure 5 to 10 µm by 0.28 to 0.34 µm, with one or two flat curves, and have 10 to 14 periplasmic flagella inserted at each cell end. Using a comprehensive evaluation of genotypic (gene comparisons and multilocus sequence typing and analysis), genomic (dDDH, ANI, and AAI) and phenotypic (hemolysis, biochemical profiles, protein spectra, antibiogram, and pathogenicity) properties, we classify Brachyspira hampsonii sp. nov. as a unique species with genetically diverse yet phenotypically similar genomovars (I, II, and III). We designate the type strain NSH-16 (= ATCC BAA-2463 = NCTC 13792).
[Mh] Termos MeSH primário: Brachyspira/classificação
Colite/veterinária
Disenteria/veterinária
Genoma Bacteriano/genética
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos/genética
Animais
Sequência de Bases/genética
Brachyspira/genética
Brachyspira/isolamento & purificação
Brachyspira/ultraestrutura
Colite/microbiologia
Disenteria/microbiologia
Microscopia Eletrônica
Tipagem de Sequências Multilocus
RNA Ribossômico 16S/genética
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE


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[PMID]:27599925
[Au] Autor:Scherrer S; Borgström A; Frei D; Wittenbrink MM
[Ad] Endereço:Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 270, CH 8057 Zurich, Switzerland. Electronic address: simone.scherrer@vetbakt.uzh.ch.
[Ti] Título:First screening for Brachyspira hampsonii in Swiss pigs applying a new high resolution melting assay.
[So] Source:Vet Microbiol;193:17-21, 2016 Sep 25.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new High Resolution Melting (HRM) assay was developed for the rapid detection of Brachyspira (B.) hampsonii. B. hampsonii occurs in different European countries, however, until today it has not been encountered in Switzerland. Four B. hampsonii reference strains were used to develop the HRM assay: B. hampsonii clade I ATCC BAA2463 and clade II ATCC BAA2464 strain, as well as two isolated strains P280/1 from the UK and the German isolate 5369-1x/12. A conserved region of the nox gene was used to design B. hampsonii-specific primers. The HRM melting curves for the four reference strains showed reproducible difference graphs with distinct differences between the four strains based on a slight variation between the four amplicon sequences. In addition, DNA from 22 B. hampsonii strains representing four genetic B. hampsonii groups was used to validate the method. Melting temperatures in the interval between 73.1 and 74°C were obtained for all B. hampsonii strains and allow differentiating B. hampsonii from other Brachyspira species. In total 897 Swiss porcine fecal Brachyspira isolates, cultured between 2009 and 2015, were analysed by the HRM protocol. B. hampsonii was not detected among these Swiss Brachyspira isolates. In conclusion, the rapid and low-cost HRM approach allows a sensitive and specific identification of B. hampsonii.
[Mh] Termos MeSH primário: Brachyspira/isolamento & purificação
Infecções por Bactérias Gram-Negativas/veterinária
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Animais
Brachyspira/genética
Fezes/microbiologia
Infecções por Bactérias Gram-Negativas/diagnóstico
Infecções por Bactérias Gram-Negativas/microbiologia
Alinhamento de Sequência/veterinária
Análise de Sequência de DNA/veterinária
Suínos
Doenças dos Suínos/diagnóstico
Suíça
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE


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[PMID]:27581381
[Au] Autor:Ogata S; Shimizu K; Oda T; Tominaga S; Nakanishi K
[Ad] Endereço:Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Saitama, 359-8513, Japan; Department of Diagnostic Pathology, JCHO Saitama Medical Center, Saitama, 330-0074, Japan. Electronic address: sogata@ndmc.ac.jp.
[Ti] Título:Immunohistochemical detection of human intestinal spirochetosis.
[So] Source:Hum Pathol;58:128-133, 2016 Dec.
[Is] ISSN:1532-8392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human intestinal spirochetosis (HIS) is a colorectal infection by Brachyspira species of spiral bacteria. Immunohistochemical cross-reaction to an antibody for Treponema pallidum aids its histologic diagnosis. This study's aim was to analyze the immunohistochemical characteristics of HIS. In this analysis, on 223 specimens from 83 HIS cases, we focused on so-called fringe formation (a histologic hallmark of HIS), spiral organisms within mucus or within crypts, and strong immunopositive materials in the mucosa, together with their location and the types of lesions. Fringe formation was found in 81.6% of all specimens and spiral organisms within mucus or within crypts in 97.3% and 57.0%, respectively. Strong immunopositive materials were observed in the surface epithelial layer in 87.9%, in the subepithelial layer in 94.6%, and in deeper mucosa in 2.2% of all specimens. The positive rates in conventional adenomas (24.0%, n = 146) and hyperplastic nodules (100%, n = 17) were each different from that found in inflammation (70.8%, n = 24), and spiral organisms were seen more frequently in the right-side large intestine than in the left (within mucus, 100%, n = 104 versus 95.0%, n = 119; within crypts, 65.4%, n = 104 versus 49.6%, n = 119). Thus, immunohistochemistry was effective not only in supporting the diagnosis of HIS but also in highlighting spiral organisms within mucus or crypts that were invisible in routine histology. Possibly, these spiral organisms may spread throughout the entire large intestine, although there is a potential problem with antibody specificity.
[Mh] Termos MeSH primário: Brachyspira/imunologia
Infecções por Bactérias Gram-Negativas/microbiologia
Imuno-Histoquímica
Enteropatias/microbiologia
Mucosa Intestinal/microbiologia
Intestino Grosso/microbiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Anticorpos/imunologia
Especificidade de Anticorpos
Feminino
Infecções por Bactérias Gram-Negativas/diagnóstico
Seres Humanos
Enteropatias/diagnóstico
Mucosa Intestinal/patologia
Intestino Grosso/patologia
Masculino
Meia-Idade
Valor Preditivo dos Testes
Reprodutibilidade dos Testes
Treponema pallidum/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160902
[St] Status:MEDLINE


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Fotocópia
[PMID]:27571948
[Au] Autor:van Hulsteijn LT; van Buren M
[Ad] Endereço:Department of Internal Medicine, Haga Hospital, The Hague, the Netherlands.
[Ti] Título:Chronic diarrhoea in a 26-year-old man.
[So] Source:Neth J Med;74(7):316, 2016 Aug.
[Is] ISSN:1872-9061
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Mh] Termos MeSH primário: Diarreia/etiologia
Infecções por Bactérias Gram-Negativas/complicações
[Mh] Termos MeSH secundário: Adulto
Brachyspira
Doença Crônica
Colo/patologia
Colonoscopia
Diarreia/diagnóstico
Diarreia/patologia
Infecções por Bactérias Gram-Negativas/diagnóstico
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/patologia
Seres Humanos
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE



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