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[PMID]:28792366
[Au] Autor:Habib N; Khan IU; Hussain F; Zhou EM; Xiao M; Dong L; Zhi XY; Li WJ
[Ad] Endereço:1​Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, PR China.
[Ti] Título:Meiothermus luteus sp. nov., a slightly thermophilic bacterium isolated from a hot spring.
[So] Source:Int J Syst Evol Microbiol;67(8):2910-2914, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A slightly thermophilic, aerobic bacterium, designated YIM 72257T, was isolated from a sediment sample taken from a hot spring in Tengchong county, Yunnan province, south-west China. The isolate was Gram-stain-negative, non-sporulating and forms non-motile rods, appearing in chains. The isolate grew at 50-65 °C, pH 6.0-9.0 and with 0.5-1 % NaCl (w/v). 16S rRNA gene sequence analysis showed that strain YIM 72257T was most closely related to Meiothermus cateniformans LY1T (95.6 %), Meiothermus ruber DSM 1279T (95.1 %) and Meiothermus taiwanensis WR-30T (94.6 %). The genomic DNA G+C content of strain YIM 72257T was 62.6 mol%. The main cellular fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0, iso-C17 : 0, iso-C15 : 0 and C16 : 0. The polar lipids consisted of an uncharacterized phospholipid and two glycolipids. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain YIM 72257T is proposed to be a representative of a novel species of the genus Meiothermus, for which the name Meiothermus luteus sp. nov. is proposed. The type strain is YIM 72257T (=KCTC 52599T=CCTCC AB 2017100T).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Bacilos e Cocos Aeróbios Gram-Negativos/classificação
Fontes Termais/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Bacilos e Cocos Aeróbios Gram-Negativos/genética
Bacilos e Cocos Aeróbios Gram-Negativos/isolamento & purificação
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002040


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[PMID]:26873585
[Au] Autor:Pascual J; García-López M; Bills GF; Genilloud O
[Ad] Endereço:Fundación MEDINA, Avenida del Conocimiento 3, Health Sciences Technology Park, 18016 Granada, Spain.
[Ti] Título:Longimicrobium terrae gen. nov., sp. nov., an oligotrophic bacterium of the under-represented phylum Gemmatimonadetes isolated through a system of miniaturized diffusion chambers.
[So] Source:Int J Syst Evol Microbiol;66(5):1976-85, 2016 May.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel chemo-organoheterotroph bacterium, strain CB-286315T, was isolated from a Mediterranean forest soil sampled at the Sierra de Tejeda, Almijara and Alhama Natural Park, Spain, by using the diffusion sandwich system, a device with 384 miniature diffusion chambers. 16S rRNA gene sequence analyses identified the isolate as a member of the under-represented phylum Gemmatimonadetes, where 'Gemmatirosa kalamazoonensis' KBS708, Gemmatimonas aurantiaca T-27T and Gemmatimonas phototrophica AP64T were the closest relatives, with respective similarities of 84.4, 83.6 and 83.3 %. Strain CB-286315T was characterized as a Gram-negative, non-motile, short to long rod-shaped bacterium. Occasionally, some cells attained an unusual length, up to 35-40 µm. The strain showed positive responses for catalase and cytochrome-c oxidase and division by binary fission, and exhibited an aerobic metabolism, showing optimal growth under normal atmospheric conditions. Strain CB-286315T was also able to grow under micro-oxic atmospheres, but not under anoxic conditions. The strain is a slowly growing bacterium able to grow under low nutrient concentrations. Major fatty acids included iso-C17 : 1ω9c, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and iso-C17 : 0. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified glycolipids and three phospholipids. The major isoprenoid quinone was MK-8 and the diagnostic diamino acid was meso-diaminopimelic acid. The DNA G+C content was 67.0 mol%. Based on a polyphasic taxonomic characterization, strain CB-286315T represents a novel genus and species, Longimicrobium terrae gen. nov., sp. nov., within the phylum Gemmatimonadetes. The type strain of Longimicrobium terrae is strain CB-286315T ( = DSM 29007T = CECT 8660T). In order to classify the novel taxon within the existing taxonomic framework, the family Longimicrobiaceae fam. nov., order Longimicrobiales ord. nov. and class Longimicrobia classis nov. are also proposed.
[Mh] Termos MeSH primário: Bacilos e Cocos Aeróbios Gram-Negativos/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Complexo IV da Cadeia de Transporte de Elétrons/genética
Ácidos Graxos/química
Florestas
Bacilos e Cocos Aeróbios Gram-Negativos/genética
Bacilos e Cocos Aeróbios Gram-Negativos/isolamento & purificação
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espanha
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-38-6 (vitamin MK 8); 583-93-7 (Diaminopimelic Acid); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160214
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.000974


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[PMID]:26809714
[Au] Autor:Ko JK; Ko H; Kim KH; Choi IG
[Ad] Endereço:Clean Energy Research Center, Korea Institute of Science and Technology, Seoul, 136-791, Republic of Korea.
[Ti] Título:Characterization of the biochemical properties of recombinant Xyn10C from a marine bacterium, Saccharophagus degradans 2-40.
[So] Source:Bioprocess Biosyst Eng;39(4):677-84, 2016 Apr.
[Is] ISSN:1615-7605
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Endo-1,4-ß-xylanases are mostly classified into glycoside hydrolase (GH) family 10 or 11. In this study, we examined the catalytic functions of a recombinant endo-1,4-ß-xylanase belonging to GH10 (Xyn10C) from a marine bacterium, Saccharophagus degradans 2-40. Optimal activity of this enzyme was evident at 30 °C and pH 7.0, but activity remained even at low temperatures, indicating its adaptation to cold. With respect to other xylanases known to be active in cold temperatures, Xyn10C is unique in that it showed maximal activity in the presence of 2 M of NaCl. The action patterns of recombinant Xyn10C on xylans from hardwood and softwood differed in part, but the enzyme hydrolyzed polysaccharidic substrates primarily to xylobiose and xylotriose through xylo-oligosaccharides, releasing a small amount of xylose. The K m and V max values on birchwood xylan were 10.4 mg mL(-1) and 253 µmol mg(-1) min(-1), respectively. The efficient catalytic function of Xyn10C on short-length xylo-oligosaccharide chains was similar to the typical function of other known GH10 xylanases.
[Mh] Termos MeSH primário: Organismos Aquáticos/enzimologia
Proteínas de Bactérias/química
Glicosídeo Hidrolases/química
Bacilos e Cocos Aeróbios Gram-Negativos/enzimologia
Xilanos/química
[Mh] Termos MeSH secundário: Organismos Aquáticos/genética
Proteínas de Bactérias/genética
Glicosídeo Hidrolases/genética
Bacilos e Cocos Aeróbios Gram-Negativos/genética
Temperatura Alta
Concentração de Íons de Hidrogênio
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Proteins); 0 (Xylans); EC 3.2.1.- (Glycoside Hydrolases)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160127
[St] Status:MEDLINE
[do] DOI:10.1007/s00449-016-1548-2


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[PMID]:26659214
[Au] Autor:Schulthess B; Bloemberg GV; Zbinden A; Mouttet F; Zbinden R; Böttger EC; Hombach M
[Ad] Endereço:Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland schulthe@imm.uzh.ch.
[Ti] Título:Evaluation of the Bruker MALDI Biotyper for Identification of Fastidious Gram-Negative Rods.
[So] Source:J Clin Microbiol;54(3):543-8, 2016 Mar.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has entered clinical laboratories, facilitating identification of bacteria. Here, we evaluated the MALDI Biotyper (Bruker Daltonics) for the identification of fastidious Gram-negative rods (GNR). Three sample preparation methods, direct colony transfer, direct transfer plus on-target formic acid preparation, and ethanol-formic acid extraction, were analyzed for 151 clinical isolates. Direct colony transfer applied with the manufacturer's interpretation criteria resulted in overall species and genus identification rates of 43.0% and 32.5%, respectively; 23.2% of the isolates were not identified, and two misidentifications (1.3%) were observed. The species identification rates increased to 46.4% and 53.7% for direct transfer plus formic acid preparation and ethanol-formic acid extraction, respectively. In addition, we evaluated score value cutoff alterations. The identification rates hardly increased by reducing the genus cutoff, while reducing the 2.0 species cutoff to 1.9 and to 1.8 increased the identification rates to up to 66.2% without increasing the rate of misidentifications. This study shows that fastidious GNR can reliably be identified using the MALDI Biotyper. However, the identification rates do not reach those of nonfastidious GNR such as the Enterobacteriaceae. In addition, two approaches optimizing the identification of fastidious GNR by the MALDI Biotyper were demonstrated: formic acid-based on-target sample treatment and reductions in cutoff scores to increase the species identification rates.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos
Bacilos e Cocos Aeróbios Gram-Negativos/classificação
Bacilos e Cocos Aeróbios Gram-Negativos/isolamento & purificação
Infecções por Bactérias Gram-Negativas/diagnóstico
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Seres Humanos
Sensibilidade e Especificidade
Manejo de Espécimes/métodos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE
[do] DOI:10.1128/JCM.03107-15


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[PMID]:26630146
[Au] Autor:James J; Kaul DR; Goldberger ZD; Saint S; Skerrett SJ
[Ti] Título:CLINICAL PROBLEM-SOLVING. Back to Nature.
[So] Source:N Engl J Med;373(23):2271-6, 2015 Dec 03.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Francisella tularensis/isolamento & purificação
Hospedeiro Imunocomprometido
Pulmão/patologia
Tularemia/diagnóstico
[Mh] Termos MeSH secundário: Adulto
Anorexia/etiologia
Antibacterianos/uso terapêutico
Ciprofloxacino/uso terapêutico
Colite Ulcerativa/complicações
Diagnóstico Diferencial
Exantema/etiologia
Febre/etiologia
Bacilos e Cocos Aeróbios Gram-Negativos/isolamento & purificação
Cefaleia/etiologia
Seres Humanos
Pulmão/diagnóstico por imagem
Masculino
Radiografia
Tularemia/complicações
Tularemia/tratamento farmacológico
[Pt] Tipo de publicação:CASE REPORTS; CLINICAL CONFERENCE; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 5E8K9I0O4U (Ciprofloxacin)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:151203
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMcps1407030


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[PMID]:26619252
[Au] Autor:Zabielska J; Kunicka-Styczynska A; Rajkowska K; Tyfa A
[Ad] Endereço:Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lódz, Poland.
[Ti] Título:Opportunistic Gram-negative rods' capability of creating biofilm structures on polivynyl chloride and styrene-acronitrile copolymer surfaces.
[So] Source:Acta Biochim Pol;62(4):733-7, 2015.
[Is] ISSN:1734-154X
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Biofilms are highly organized microbial communities displaying high resistance to disinfectants and other external environmental factors. Medical equipment, such as stents and catheters, can be colonized by a variety of bacteria including opportunistic pathogens circulating in the environment and dangerous to immunocompromised patients. Application of materials resistant to biofilm formation will minimize the risk of patients' infection. Hence, the aim of this research was to determine the biofilm growth of environmental bacteria isolates on polyvinyl chloride and styrene-acronitrile copolymer surfaces. Nine strains (Pseudomonas aeruginosa, Burkholderia cepacia and Serratia liquefacies) isolated from cosmetics, and a reference P. aeruginosa strain ATCC 15442, were tested. The ability and dynamics of biofilm formation on intubation catheters (30°C, up to 24 h) in bacterial growth cultures (10(7)-10(8) CFU/ml) was investigated, with subsequent sonication and quantification by agar plate count method. The results indicated that all the tested bacteria expressed a strong ability for the polymer surface adhesion, reaching 4.6 to 6.7 log CFU/cm(2) after 30 minutes. Moreover, for the majority of strains, the level of 24-hour biofilm production was from 6.67-7.61 log CFU/cm(2). This research indicates that the environmental strains circulating between the cosmetics and patients may pose a threat of biofilm formation on medical equipment surfaces, and presumably in the clinical surroundings as well.
[Mh] Termos MeSH primário: Biofilmes
Bacilos e Cocos Aeróbios Gram-Negativos/fisiologia
Nitrilos/química
Polímeros/química
Cloreto de Polivinila
[Mh] Termos MeSH secundário: Aderência Bacteriana
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitriles); 0 (Polymers); 9002-86-2 (Polyvinyl Chloride)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151217
[Lr] Data última revisão:
151217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151201
[St] Status:MEDLINE
[do] DOI:10.18388/abp.2015_1121


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[PMID]:26552126
[Au] Autor:Murase K; Noda K; Otaki Y; Yasuda J; Oda T; Yokozawa T; Kikuchi K
[Ti] Título:[A Case of Bacteremia Which Followed a Urinary Tract Infection by Oligella urethralis].
[So] Source:Kansenshogaku Zasshi;89(2):274-8, 2015 Mar.
[Is] ISSN:0387-5911
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:An 82-year-old bedridden man with sequelae from a cerebral infarction was admitted to a welfare institution for the elderly. He developed a high fever and hematuria and was prescribed levofloxacin for the treatment of a suspected urinary tract infection. Although his condition improved, the symptoms subsequently recurred; therefore, levofloxacin was again administered. He remained febrile and was admitted to a hospital due to recalcitrant urinary tract infection. Immediately after admission, he developed ischuria and pyuria. Urine and blood cultures at admission indicated the presence of levofloxacin-resistant Oligella urethralis (O. urethralis). He recovered with ceftriaxone medication. To our knowledge, this is the first report of bacteremia associated with a urinary tract infection caused by O. urethralis in Japan.
[Mh] Termos MeSH primário: Bacteriemia/etiologia
Bacilos e Cocos Aeróbios Gram-Negativos
Infecções por Bactérias Gram-Negativas
Infecções Urinárias/complicações
[Mh] Termos MeSH secundário: Idoso de 80 Anos ou mais
Seres Humanos
Masculino
[Pt] Tipo de publicação:CASE REPORTS; ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Mês de entrada:1601
[Cu] Atualização por classe:161201
[Lr] Data última revisão:
161201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151111
[St] Status:MEDLINE


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[PMID]:26398627
[Au] Autor:Muratova A; Dubrovskaya E; Golubev S; Grinev V; Chernyshova M; Turkovskaya O
[Ad] Endereço:Institute of Biochemistry and Physiology of Plants and Microorganisms, RAS, 13 Prospekt Entuziastov, 410049 Saratov, Russia. Electronic address: amuratova@yahoo.com.
[Ti] Título:The coupling of the plant and microbial catabolisms of phenanthrene in the rhizosphere of Medicago sativa.
[So] Source:J Plant Physiol;188:1-8, 2015 Sep 01.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We studied the catabolism of the polycyclic aromatic hydrocarbon phenanthrene by four rhizobacterial strains and the possibility of enzymatic oxidation of this compound and its microbial metabolites by the root exudates of alfalfa (Medicago sativa L.) in order to detect the possible coupling of the plant and microbial metabolisms under the rhizospheric degradation of the organic pollutant. A comparative study of phenanthrene degradation pathways in the PAH-degrading rhizobacteria Ensifer meliloti, Pseudomonas kunmingensis, Rhizobium petrolearium, and Stenotrophomonas sp. allowed us to identify the key metabolites from the microbial transformation of phenanthrene, including 9,10-phenanthrenequinone, 2-carboxybenzaldehyde, and 1-hydroxy-2-naphthoic, salicylic, and o-phthalic acids. Sterile alfalfa plants were grown in the presence and absence of phenanthrene (0.03 g kg(-1)) in quartz sand under controlled environmental conditions to obtain plant root exudates. The root exudates were collected, concentrated by ultrafiltration, and the activity of oxidoreductases was detected spectrophotometrically by the oxidation rate for various substrates. The most marked activity was that of peroxidase, whereas the presence of oxidase and tyrosinase was detected on the verge of the assay sensitivity. Using alfalfa root exudates as a crude enzyme preparation, we found that in the presence of the synthetic mediator, the plant peroxidase could oxidize phenanthrene and its microbial metabolites. The results indicate the possibility of active participation of plants in the rhizospheric degradation of polycyclic aromatic hydrocarbons and their microbial metabolites, which makes it possible to speak about the coupling of the plant and microbial catabolisms of these contaminants in the rhizosphere.
[Mh] Termos MeSH primário: Bacilos e Cocos Aeróbios Gram-Negativos/metabolismo
Medicago sativa/metabolismo
Medicago sativa/microbiologia
Fenantrenos/metabolismo
Microbiologia do Solo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
Pseudomonas/metabolismo
Rhizobiaceae/metabolismo
Rizosfera
Stenotrophomonas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Phenanthrenes); 0 (Soil Pollutants); 448J8E5BST (phenanthrene)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151103
[Lr] Data última revisão:
151103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150924
[St] Status:MEDLINE


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[PMID]:26303908
[Au] Autor:Choi A; Song J; Joung Y; Kogure K; Cho JC
[Ad] Endereço:1​Department of Biological Sciences, Inha University, Incheon 40-751, Republic of Korea.
[Ti] Título:Lentisphaera profundi sp. nov., isolated from deep-sea water.
[So] Source:Int J Syst Evol Microbiol;65(11):4186-4190, 2015 Nov.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-staining-negative, aerobic, non-motile, coccus-shaped bacterium, designated SAORIC-696T, was isolated from deep-sea water at a depth of 1700 m in the western North Pacific Ocean. Optimal growth of strain SAORIC-696T was observed at 15 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. Strain SAORIC-696T formed a robust phylogenetic clade with members of the genus Lentisphaera. The 16S rRNA gene sequence similarity showed that strain SAORIC-696T was most closely related to Lentisphaera marina (98.0 % similarity) and Lentisphaera araneosa (97.3 %). The DNA-DNA relatedness between SAORIC-696T and two species of the genus Lentisphaera was only 27-42 %. The DNA G+C content of strain SAORIC-696T was 43.1 mol% and predominant cellular fatty acids were C16 : 1ω9c (36.8 %), C14 : 0 (22.5 %) and C14 : 0 3-OH and/or iso-C16 : 1 I (10.8 %). Strain SAORIC-696T contained MK-7 as the only respiratory quinone. On the basis of taxonomic data collected in this study, it was concluded that strain SAORIC-696T represents a novel species of the genus Lentisphaera, for which the name Lentisphaera profundi sp. nov. is proposed, with the type strain SAORIC-696T ( = NBRC 110692T = KCTC 42681T).
[Mh] Termos MeSH primário: Bacilos e Cocos Aeróbios Gram-Negativos/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Bactérias/genética
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Bacilos e Cocos Aeróbios Gram-Negativos/genética
Bacilos e Cocos Aeróbios Gram-Negativos/isolamento & purificação
Hibridização de Ácido Nucleico
Oceano Pacífico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150826
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.000556


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[PMID]:25607672
[Au] Autor:Liu R; Gao Y; Ji Y; Zhang Y; Yang M
[Ad] Endereço:College of Environmental and Resource Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China E-mail: yingxingao@sina.com.
[Ti] Título:Characteristics of hydrocarbon hydroxylase genes in a thermophilic aerobic biological system treating oily produced wastewater.
[So] Source:Water Sci Technol;71(1):75-82, 2015.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Alkane and aromatic hydroxylase genes in a full-scale aerobic system treating oily produced wastewater under thermophilic condition (45-50 °C) in the Jidong oilfield, China, were investigated using clone library and quantitative polymerase chain reaction methods. Rather than the normally encountered integral-membrane non-haem iron monooxygenase (alkB) genes, only CYP153-type P450 hydroxylase genes were detected for the alkane activation, indicating that the terminal oxidation of alkanes might be mainly mediated by the CYP153-type alkane hydroxylases in the thermophilic aerobic process. Most of the obtained CYP153 gene clones showed distant homology with the reference sequences, which might represent novel alkane hydroxylases. For the aromatic activation, the polycyclic aromatic hydrocarbon-ring hydroxylating dioxygenase (PAH-RHD) gene was derived from Gram-negative PAH-degraders belonging to the Burkholderiales order, with a 0.72% relative abundance of PAH-RHD gene to 16S rRNA gene. This was consistent with the result of 16S rRNA gene analysis, indicating that Burkholderiales bacteria might play a key role in the full-scale process of thermophilic hydrocarbon degradation.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Betaproteobacteria/enzimologia
Betaproteobacteria/genética
Citocromo P-450 CYP4A/genética
Dioxigenases/genética
Bacilos e Cocos Aeróbios Gram-Negativos/enzimologia
Bacilos e Cocos Aeróbios Gram-Negativos/genética
[Mh] Termos MeSH secundário: Aerobiose
Proteínas de Bactérias/metabolismo
Reatores Biológicos/microbiologia
Citocromo P-450 CYP4A/metabolismo
Dioxigenases/metabolismo
Temperatura Alta
Oxigenases de Função Mista/genética
Oxigenases de Função Mista/metabolismo
Dados de Sequência Molecular
Filogenia
RNA Ribossômico 16S/genética
RNA Ribossômico 16S/metabolismo
Análise de Sequência de DNA
Eliminação de Resíduos Líquidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (RNA, Ribosomal, 16S); EC 1.- (Mixed Function Oxygenases); EC 1.13.11.- (Dioxygenases); EC 1.14.15.3 (Cytochrome P-450 CYP4A)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150122
[Lr] Data última revisão:
150122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150122
[St] Status:MEDLINE
[do] DOI:10.2166/wst.2014.470



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