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[PMID]:28551873
[Au] Autor:Singh P; Kim YJ; Singh H; Farh ME; Yang DC
[Ad] Endereço:Department of Oriental Medicine Biotechnology, Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 17104, Republic of Korea. prnksingh254@gmail.com.
[Ti] Título:Achromobacter panacis sp. nov., isolated from rhizosphere of Panax ginseng.
[So] Source:J Microbiol;55(6):428-434, 2017 Jun.
[Is] ISSN:1976-3794
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:A novel strain DCY105 was isolated from soil collected from the rhizosphere of ginseng (Panax ginseng), in Gochang, Republic of Korea. Strain DCY105 is Gram-reaction-negative, white, non-motile, non-flagellate, rod-shaped and aerobic. The bacteria grow optimally at 30°C, pH 6.5-7.0 and in the absence of NaCl. Phylogenetically, strain DCY105 is most closely related to Achromobacter marplatensis LMG 26219 (96.81%). The DNA G+C content of strain DCY105 was 64.4 mol%. Ubiquinone 8 was the major respiratory quinone, and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were amongst the major polar lipids. C , C 3OH and iso-C 3OH were identified as the major fatty acids present in DCY105 . The results of physiological and biochemical tests allowed strain DCY105 to be differentiated phenotypically from other recognized species belonging to the genus Achromobacter. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Achromobacter panacis sp. nov. is proposed with the type strain designated as DCY105 (=CCTCCAB 2015193 =KCTC 42751 ).
[Mh] Termos MeSH primário: Achromobacter
Panax/microbiologia
[Mh] Termos MeSH secundário: Achromobacter/classificação
Achromobacter/genética
Achromobacter/isolamento & purificação
Composição de Bases/genética
DNA Bacteriano/genética
Ácidos Graxos/análise
RNA Ribossômico 16S/genética
República da Coreia
Rizosfera
Análise de Sequência de DNA
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170529
[St] Status:MEDLINE
[do] DOI:10.1007/s12275-017-6612-3


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[PMID]:28357512
[Au] Autor:Dreiseikelmann B; Bunk B; Spröer C; Rohde M; Nimtz M; Wittmann J
[Ad] Endereço:Department of Microbiology/Genetechnology, University of Bielefeld, 33615, Bielefeld, Germany.
[Ti] Título:Characterization and genome comparisons of three Achromobacter phages of the family Siphoviridae.
[So] Source:Arch Virol;162(8):2191-2201, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:In this study, we present the characterization and genomic data of three Achromobacter phages belonging to the family Siphoviridae. Phages 83-24, JWX and JWF were isolated from sewage samples in Paris and Braunschweig, respectively, and infect Achromobacter xylosoxidans, an emerging nosocomial pathogen in cystic fibrosis patients. Analysis of morphology and growth parameters revealed that phages 83-24 and JWX have similar properties, both have nearly the same head and tail measurements, and both have a burst size between 85 and 100 pfu/cell. In regard to morphological properties, JWF had a much longer and more flexible tail compared to other phages. The linear double-stranded DNAs of all three phages are terminally redundant and not circularly permutated. The complete nucleotide sequences consist of 81,541 bp for JWF, 49,714 bp for JWX and 48,216 bp for 83-24. Analysis of the genome sequences showed again that phages JWX and 83-24 are quite similar. Comparison to the GenBank database via BLASTN revealed partial similarities to Roseobacter phage RDJL phi1 and Burkholderia phage BcepGomr. In contrast, BLASTN analysis of the genome sequence of phage JWF revealed only few similarities to non-annotated prophage regions in different strains of Burkholderia and Mesorhizobium.
[Mh] Termos MeSH primário: Achromobacter/virologia
Genoma Viral
Siphoviridae/classificação
[Mh] Termos MeSH secundário: Sequência de Bases
Mapeamento Cromossômico
Análise de Sequência de DNA
Siphoviridae/genética
Siphoviridae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3347-8


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[PMID]:28184965
[Au] Autor:Ermakova IT; Shushkova TV; Sviridov AV; Zelenkova NF; Vinokurova NG; Baskunov BP; Leontievsky AA
[Ad] Endereço:G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 5 Prospect Nauki, Pushchino, Moscow, 142290, Russia.
[Ti] Título:Organophosphonates utilization by soil strains of Ochrobactrum anthropi and Achromobacter sp.
[So] Source:Arch Microbiol;199(5):665-675, 2017 Jul.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Four bacterial strains from glyphosate- or alkylphosphonates-contaminated soils were tested for ability to utilize different organophosphonates. All studied strains readily utilized methylphosphonic acid and a number of other phosphonates, but differed in their ability to degrade glyphosate. Only strains Ochrobactrum anthropi GPK 3 and Achromobacter sp. Kg 16 utilized this compound after isolation from enrichment cultures with glyphosate. Achromobacter sp. MPK 7 from the same enrichment culture, similar to Achromobacter sp. MPS 12 from methylphosphonate-polluted source, required adaptation to growth on GP. Studied strains varied significantly in their growth parameters, efficiency of phosphonates degradation and characteristic products of this process, as well as in their energy metabolism. These differences give grounds to propose a possible model of interaction between these strains in microbial consortium in phosphonate-contaminated soils.
[Mh] Termos MeSH primário: Achromobacter/metabolismo
Biodegradação Ambiental
Glicina/análogos & derivados
Ochrobactrum anthropi/metabolismo
Organofosfonatos/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Glicina/metabolismo
Consórcios Microbianos
Compostos Organofosforados/metabolismo
Solo/química
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Organophosphonates); 0 (Organophosphorus Compounds); 0 (Soil); 0 (Soil Pollutants); 329W4YM10Z (methylphosphonic acid); 4632WW1X5A (glyphosate); TE7660XO1C (Glycine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-017-1343-8


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[PMID]:28154986
[Au] Autor:Wu ZG; Wang F; Ning LQ; Stedtfeld RD; Yang ZZ; Cao JG; Sheng HJ; Jiang X
[Ad] Endereço:College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology, Tianjin, 300457, People's Republic of China.
[Ti] Título:Biodegradation of 5-chloro-2-picolinic acid by novel identified co-metabolizing degrader Achromobacter sp. f1.
[So] Source:Biodegradation;28(2-3):139-144, 2017 Jun.
[Is] ISSN:1572-9729
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Several bacteria have been isolated to degrade 4-chloronitrobenzene. Degradation of 4-chloronitrobenzene by Cupriavidus sp. D4 produces 5-chloro-2-picolinic acid as a dead-end by-product, a potential pollutant. To date, no bacterium that degrades 5-chloro-2-picolinic acid has been reported. Strain f1, isolated from a soil polluted by 4-chloronitrobenzene, was able to co-metabolize 5-chloro-2-picolinic acid in the presence of ethanol or other appropriate carbon sources. The strain was identified as Achromobacter sp. based on its physiological, biochemical characteristics, and 16S rRNA gene sequence analysis. The organism completely degraded 50, 100 and 200 mg L of 5-chloro-2-picolinic acid within 48, 60, and 72 h, respectively. During the degradation of 5-chloro-2-picolinic acid, Cl was released. The initial metabolic product of 5-chloro-2-picolinic acid was identified as 6-hydroxy-5-chloro-2-picolinic acid by LC-MS and NMR. Using a mixed culture of Achromobacter sp. f1 and Cupriavidus sp. D4 for degradation of 4-chloronitrobenzen, 5-chloro-2-picolinic acid did not accumulate. Results infer that Achromobacter sp. f1 can be used for complete biodegradation of 4-chloronitrobenzene in remedial applications.
[Mh] Termos MeSH primário: Achromobacter/metabolismo
Ácidos Picolínicos/metabolismo
[Mh] Termos MeSH secundário: Achromobacter/isolamento & purificação
Biodegradação Ambiental
Cromatografia Líquida
Técnicas de Cocultura
Cupriavidus/metabolismo
Hidroxilação
Cinética
Espectrometria de Massas
Metaboloma
Nitrobenzenos/metabolismo
Espectroscopia de Prótons por Ressonância Magnética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-chloro-2-picolinic acid); 0 (Nitrobenzenes); 0 (Picolinic Acids); CVL66U249D (4-chloronitrobenzene); QZV2W997JQ (picolinic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1007/s10532-017-9783-z


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[PMID]:28124776
[Au] Autor:Shi Y; Li C; Yang H; Zhang T; Gao Y; Chu M; Zeng J; Lin Q; OuTiKuEr; Li Y; Huo X; Lou K
[Ad] Endereço:Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.
[Ti] Título:Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar beet.
[So] Source:J Microbiol;55(4):267-272, 2017 Apr.
[Is] ISSN:1976-3794
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:This study details the introduction of a gfp marker into an endophytic bacterial strain (Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its colonization of sugar beet (Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days. gfp-Expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the Achromobacter marplatensis 17:gfp strain in plants 20 days after inoculation, even in the absence of selective pressure, suggests that it is good colonizer. These results also suggest that this strain could be a useful tool for the delivery of enzymes or other proteins into plants. In addition, the study highlights that sugar beet plants can be used effectively for detailed in vitro studies on the interactions between A. marplatensis strain 17 and its host, particularly if a gfp-tagged strain of the pathogen is used.
[Mh] Termos MeSH primário: Achromobacter/crescimento & desenvolvimento
Beta vulgaris/microbiologia
Endófitos/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Achromobacter/química
Achromobacter/genética
Endófitos/química
Endófitos/genética
Instabilidade Genômica
Proteínas de Fluorescência Verde/análise
Proteínas de Fluorescência Verde/genética
Microscopia de Fluorescência
Raízes de Plantas/microbiologia
Caules de Planta/microbiologia
Plasmídeos
Proteínas Recombinantes/análise
Proteínas Recombinantes/genética
Coloração e Rotulagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1007/s12275-017-6371-1


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[PMID]:27933337
[Au] Autor:Xia ZY; Zhang L; Zhao Y; Yan X; Li SP; Gu T; Jiang JD
[Ad] Endereço:Yunnan Academy of Tobacco Agricultural Science, Kunming, 650031, People's Republic of China.
[Ti] Título:Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by a New Isolated Strain of Achromobacter sp. LZ35.
[So] Source:Curr Microbiol;74(2):193-202, 2017 Feb.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, a bacterial strain of Achromobacter sp. LZ35, which was capable of utilizing 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-methyl-4-chlorophenoxy acetic acid (MCPA) as the sole sources of carbon and energy for growth, was isolated from the soil in a disused pesticide factory in Suzhou, China. The optimal 2,4-D degradation by strain LZ35 occurred at 30 °C and pH 8.0 when the initial 2,4-D concentration was 200 mg L . Strain LZ35 harbored the conserved 2,4-D/alpha-ketoglutarate dioxygenase (96%) and 2,4-dichlorophenol hydroxylase (99%), and catabolized 2,4-D via the intermediate 2,4-dichlorophenol. The inoculation of 7.8 × 10 CFU g soil of strain LZ35 cells to 2,4-D-contaminated soil could efficiently remove over 75 and 90% of 100 and 50 mg L 2,4-D in 12 days and significantly released the phytotoxicity of maize caused by the 2,4-D residue. This is the first report of an Achromobacter sp. strain that was capable of mineralizing both 2,4-D and MCPA. This study provides us a promising candidate for its application in the bioremediation of 2,4-D- or MCPA-contaminated sites.
[Mh] Termos MeSH primário: Ácido 2,4-Diclorofenoxiacético/metabolismo
Achromobacter/metabolismo
Herbicidas/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Ácido 2,4-Diclorofenoxiacético/toxicidade
Ácido 2-Metil-4-clorofenoxiacético/metabolismo
Ácido 2-Metil-4-clorofenoxiacético/toxicidade
Achromobacter/isolamento & purificação
Biotransformação
China
Enzimas/análise
Herbicidas/toxicidade
Concentração de Íons de Hidrogênio
Redes e Vias Metabólicas
Poluentes do Solo/toxicidade
Temperatura Ambiente
Fatores de Tempo
Zea mays/efeitos dos fármacos
Zea mays/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes); 0 (Herbicides); 0 (Soil Pollutants); 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid); D888C394VO (2-Methyl-4-chlorophenoxyacetic Acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-016-1173-y


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[PMID]:27902206
[Au] Autor:Kuncharoen N; Muramatsu Y; Shibata C; Kamakura Y; Nakagawa Y; Tanasupawat S
[Ad] Endereço:1​Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
[Ti] Título:Achromobacter aloeverae sp. nov., isolated from the root of Aloe vera (L.) Burm.f.
[So] Source:Int J Syst Evol Microbiol;67(1):37-41, 2017 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two Gram-staining-negative, strictly aerobic, rod-shaped bacteria, designated strains AVA-1T and AVA-2, were isolated from the root of Aloe vera (L.) Brum.f. derived from Chachoengsao Province, Thailand. The strains contained cytochrome oxidase and catalase activities. They grew in 4 % (w/v) NaCl, at a pH range of 6.0-9.0 (optimally at pH 7) and at 20-42 °C (optimally at 30-37 °C). The major isoprenoid quinone was ubiquinone with eight isoprene units (Q-8). The major fatty acids were C16 : 0 and C17 : 0 cyclo. On the basis of 16S rRNA gene sequence analysis, the strains represent a species belonging to the genus Achromobacter and are closely related to Achromobacter xylosoxidans NBRC 15126T (98.80 %), Achromobacter insolitus LMG 6003T (98.64 %), Achromobacter aminicus LMG 26690T (98.59 %), Achromobacter pulmonis LMG 26696T (98.58 %) and Achromobacter insuavis LMG 26845T (98.58 %). The DNA G+C content of strain AVA-1T was 66.5 mol%. The novel strains had low DNA-DNA relatedness values with related type strains. On the basis of the phenotypic and genotypic data obtained, the strains clearly represent a novel species, for which the name Achromobacter aloeverae sp. nov. is proposed. The type strain is strain AVA-1T (=LMG 29108T=NBRC 111463T=PCU 352T=TISTR 2383T).
[Mh] Termos MeSH primário: Achromobacter/classificação
Aloe/microbiologia
Filogenia
Raízes de Plantas/microbiologia
[Mh] Termos MeSH secundário: Achromobacter/genética
Achromobacter/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Tailândia
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001566


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[PMID]:27884748
[Au] Autor:Blanco-Moreno R; Sáez LP; Luque-Almagro VM; Roldán MD; Moreno-Vivián C
[Ad] Endereço:Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, 1ª Planta, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain.
[Ti] Título:Isolation of bacterial strains able to degrade biphenyl, diphenyl ether and the heat transfer fluid used in thermo-solar plants.
[So] Source:N Biotechnol;35:35-41, 2017 Mar 25.
[Is] ISSN:1876-4347
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Thermo-solar plants use eutectic mixtures of diphenyl ether (DE) and biphenyl (BP) as heat transfer fluid (HTF). Potential losses of HTF may contaminate soils and bioremediation is an attractive tool for its treatment. DE- or BP-degrading bacteria are known, but up to now bacteria able to degrade HTF mixture have not been described. Here, five bacterial strains which are able to grow with HTF or its separate components DE and BP as sole carbon sources have been isolated, either from soils exposed to HTF or from rhizospheric soils of plants growing near a thermo-solar plant. The organisms were identified by 16S rRNA gene sequencing as Achromobacter piechaudii strain BioC1, Pseudomonas plecoglossicida strain 6.1, Pseudomonas aeruginosa strains HBD1 and HBD3, and Pseudomonas oleovorans strain HBD2. Activity of 2,3-dihydroxybiphenyl dioxygenase (BphC), a key enzyme of the biphenyl upper degradation pathway, was detected in all isolates. Pseudomonas strains almost completely degraded 2000ppm HTF after 5-day culture, and even tolerated and grew in the presence of 150,000ppm HTF, being suitable candidates for in situ soil bioremediation. Degradation of both components of HTF is of particular interest since in the DE-degrader Sphingomonas sp. SS3, growth on DE or benzoate was strongly inhibited by addition of BP.
[Mh] Termos MeSH primário: Achromobacter/metabolismo
Compostos de Bifenilo/metabolismo
Éteres Fenílicos/metabolismo
Pseudomonas/metabolismo
[Mh] Termos MeSH secundário: Achromobacter/isolamento & purificação
Biodegradação Ambiental
Biotecnologia
Temperatura Alta
Microbiologia Industrial
Pseudomonas/isolamento & purificação
Pseudomonas aeruginosa/isolamento & purificação
Pseudomonas aeruginosa/metabolismo
Pseudomonas oleovorans/isolamento & purificação
Pseudomonas oleovorans/metabolismo
Rizosfera
Microbiologia do Solo
Energia Solar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Phenyl Ethers); 2L9GJK6MGN (diphenyl); 3O695R5M1U (phenyl ether)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170228
[Lr] Data última revisão:
170228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


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[PMID]:27873565
[Au] Autor:Pereira RH; Leão RS; Carvalho-Assef AP; Albano RM; Rodrigues ER; Firmida MC; Folescu TW; Plotkowski MC; Bernardo VG; Marques EA
[Ad] Endereço:Departamento de Microbiologia,Imunologia e Parasitologia,Faculdade de Ciências Médicas,Universidade do Estado do Rio de Janeiro,Brazil.
[Ti] Título:Patterns of virulence factor expression and antimicrobial resistance in Achromobacter xylosoxidans and Achromobacter ruhlandii isolates from patients with cystic fibrosis.
[So] Source:Epidemiol Infect;145(3):600-606, 2017 02.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Achromobacter spp. are opportunistic pathogens increasingly recovered from adult patients with cystic fibrosis (CF). We report the characterization of 122 Achromobacter spp. isolates recovered from 39 CF patients by multilocus sequence typing, virulence traits, and susceptibility to antimicrobials. Two species, A. xylosoxidans (77%) and A. ruhlandii (23%) were identified. All isolates showed a similar biofilm formation ability, and a positive swimming phenotype. By contrast, 4·3% and 44·4% of A. xylosoxidans and A. ruhlandii, respectively, exhibited a negative swarming phenotype, making the swimming and swarming abilities of A. xylosoxidans significantly higher than those of A. ruhlandii. A. xylosoxidans isolates from an outbreak clone also exhibited significantly higher motility. Both species were generally susceptible to ceftazidime, ciprofloxacin, imipenem and trimethoprim/sulphamethoxazole and there was no significant difference in susceptibility between isolates from chronic or sporadic infection. However, A. xylosoxidans isolates from chronic and sporadic cases were significantly more resistant to imipenem and ceftazidime than isolates of the outbreak clone.
[Mh] Termos MeSH primário: Achromobacter/isolamento & purificação
Fibrose Cística/complicações
Infecções por Bactérias Gram-Negativas/microbiologia
Fatores de Virulência/análise
[Mh] Termos MeSH secundário: Achromobacter/classificação
Achromobacter/efeitos dos fármacos
Achromobacter/fisiologia
Antibacterianos/farmacologia
Biofilmes/crescimento & desenvolvimento
Seres Humanos
Locomoção
Testes de Sensibilidade Microbiana
Tipagem de Sequências Multilocus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Virulence Factors)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268816002624


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[PMID]:27807149
[Au] Autor:Dupont C; Jumas-Bilak E; Michon AL; Chiron R; Marchandin H
[Ad] Endereço:Université Montpellier, UMR5569 Hydrosciences Montpellier, Equipe Pathogènes Hydriques, Santé, Environnements, UFR des Sciences Pharmaceutiques et Biologiques, Montpellier, France.
[Ti] Título:Impact of High Diversity of Achromobacter Populations within Cystic Fibrosis Sputum Samples on Antimicrobial Susceptibility Testing.
[So] Source:J Clin Microbiol;55(1):206-215, 2017 Jan.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic colonization by opportunistic environmental bacteria is frequent in the airways of cystic fibrosis (CF) patients. Studies of Pseudomonas aeruginosa evolution during persistence have highlighted the emergence of pathoadaptive genotypes and phenotypes, leading to complex and diversified inpatient colonizing populations also observed at the intraspecimen level. Such diversity, including heterogeneity in resistance profiles, has been considered an adaptive strategy devoted to host persistence. Longitudinal genomic diversity has been shown for the emergent opportunistic pathogen Achromobacter, but phenotypic and genomic diversity has not yet been studied within a simple CF sputum sample. Here, we studied the genomic diversity and antimicrobial resistance heterogeneity of 132 Achromobacter species strains (8 to 27 strains of identical or distinct colonial morphotypes per specimen) recovered from the sputum samples of 9 chronically colonized CF patients. We highlighted the high within-sample and within-morphotype diversity of antimicrobial resistance (disk diffusion) and genomic (pulsed-field gel electrophoresis) profiles. No sputum sample included strains with identical pulsotypes or antibiotic susceptibility patterns. Differences in clinical categorization were observed for the 9 patients and concerned 3 to 11 antibiotics, including antibiotics recommended for use against Achromobacter Within-sample antimicrobial resistance heterogeneity, not predictable from colonial morphology, suggested that it may represent a selective advantage against antibiotics in an Achromobacter persisting population and potentially compromise the antibiotic management of CF airway infections.
[Mh] Termos MeSH primário: Achromobacter/classificação
Achromobacter/efeitos dos fármacos
Fibrose Cística/complicações
Farmacorresistência Bacteriana
Variação Genética
Infecções por Bactérias Gram-Negativas/microbiologia
Escarro/microbiologia
[Mh] Termos MeSH secundário: Achromobacter/genética
Achromobacter/isolamento & purificação
Adolescente
Adulto
Criança
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Eletroforese em Gel de Campo Pulsado
Feminino
Genótipo
Seres Humanos
Masculino
Meia-Idade
Fenótipo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE
[do] DOI:10.1128/JCM.01843-16



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