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Pesquisa : B03.440.400.425.115.050.200 [Categoria DeCS]
Referências encontradas : 155 [refinar]
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[PMID]:28448840
[Au] Autor:Bhattacharyya D; Lee YH
[Ad] Endereço:Division of Biotechnology, Chonbuk National University, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596, Republic of Korea.
[Ti] Título:A cocktail of volatile compounds emitted from Alcaligenes faecalis JBCS1294 induces salt tolerance in Arabidopsis thaliana by modulating hormonal pathways and ion transporters.
[So] Source:J Plant Physiol;214:64-73, 2017 Jul.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In our previous study we showed that volatile organic compounds (VOCs) from Alcaligenes faecalis JBCS1294 (JBCS1294) induced tolerance to salt stress in Arabidopsis thaliana by influencing the auxin and gibberellin pathways and upregulating the expression of key ion transporters. The aim of this study was to evaluate the contribution of each VOC and blends of the VOCs on the induction of salt tolerance and signaling pathways. The key VOCs emitted from JBCS1294 were dissolved in lanolin and applied to one side of bipartite I-plates that contained Arabidopsis seeds on Murashige and Skoog (MS) media supplemented with NaCl on the other side. Changes in plant growth were investigated using Arabidopsis mutant lines and hormone inhibitors, and gene expression was assessed by real-time PCR (qPCR). Among the VOCs, butyric acid conferred salt tolerance over a concentration range of 5.6µM (10ng)-56mM (100µg), whereas propionic and benzoic acid were effective at micromolar doses. Intriguingly, the optimized cocktail of the three VOCs increased fresh weight of Arabidopsis under salt stress compared to that achieved with each single compound. However, Arabidopsis growth was not promoted by the VOCs without salt stress. Exogenous indole-3-acetic acid (IAA) application arrested salt tolerance or growth promotion of Arabidopsis induced by volatiles from propionic acid, but not from butyric acid and an optimized volatile mixture of butyric acid, propionic acid, and benzoic acid (1PBB). High and intense auxin-responsive DR5:GUS activity was observed in the roots of Arabidopsis grown on media without salt via 1PBB, butyric acid, and benzoic acid. Growth promotion by the cocktail was inhibited in the eir1 mutant and in Col-0 plants treated with inhibitors of auxin and gibberellin. The present study clearly demonstrated the effects of individual VOCs and blends of VOCs from a rhizobacterial strain on the induction of salt stress. The results with the blend of VOCs, which mimics bacterial emissions in nature, may lead to a deeper understanding of the interaction between rhizobacteria and plants.
[Mh] Termos MeSH primário: Alcaligenes faecalis/química
Arabidopsis/efeitos dos fármacos
Arabidopsis/metabolismo
Compostos Orgânicos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Giberelinas/metabolismo
Ácidos Indolacéticos/metabolismo
Tolerância a Sal/efeitos dos fármacos
Compostos Orgânicos Voláteis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gibberellins); 0 (Indoleacetic Acids); 0 (Volatile Organic Compounds)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


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[PMID]:28259060
[Au] Autor:Giovanella P; Cabral L; Costa AP; de Oliveira Camargo FA; Gianello C; Bento FM
[Ad] Endereço:Fuels and Biofuels Biodeterioration Laboratory (LAB-BIO), Departament of Microbiology, Immunology and Parasitology - Institute of Health Science, Federal University of Rio Grande do Sul, 500 Sarmento Leite St, 90050-170 Porto Alegre, RS, Brazil. Electronic address: patricia.giovanella@hotmail.com.
[Ti] Título:Metal resistance mechanisms in Gram-negative bacteria and their potential to remove Hg in the presence of other metals.
[So] Source:Ecotoxicol Environ Saf;140:162-169, 2017 Jun.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Contamination of the environment by heavy metals has been increasing in recent years due to industrial activities. Thus research involving microorganisms capable of surviving in multi-contaminated environments is extremely important. The objectives of the present study were to evaluate the removal of mercury alone and in the presence of cadmium, nickel and lead by four mercury-resistant microorganisms; estimate the removal of Cd, Ni and Pb; understand the mechanisms involved (reduction, siderophores, biofilms, biosorption and bioaccumulation) in the metal resistance of the isolate Pseudomonas sp. B50D; and determine the capacity of Pseudomonas sp. B50D in removing Hg, Cd, Ni and Pb from an industrial effluent. It was shown that the four isolates evaluated were capable of removing from 62% to 95% of mercury from a culture medium with no addition of other metals. The isolate Pseudomonas sp. B50D showed the best performance in the removal of mercury when evaluated concomitantly with other metals. This isolate was capable of removing 75% of Hg in the presence of Cd and 91% in the presence of Ni and Pb. With respect to the other metals it removed 60%, 15% and 85% of Cd, Ni and Pb, respectively. In tests with effluent, the isolate Pseudomonas sp. B50D removed 85% of Hg but did not remove the other metals. This isolate presented reduction, biosorption, biofilm production and siderophore production as its metal resistance mechanisms. Pseudomonas sp. B50D was thus a candidate with potential for application in the bioremediation of effluents with complex metal contaminations.
[Mh] Termos MeSH primário: Adaptação Fisiológica/fisiologia
Mercúrio/metabolismo
Pseudomonas putida/metabolismo
Pseudomonas/metabolismo
[Mh] Termos MeSH secundário: Alcaligenes faecalis/isolamento & purificação
Alcaligenes faecalis/metabolismo
Biodegradação Ambiental
Biofilmes
Cádmio/metabolismo
Resíduos Industriais
Chumbo/metabolismo
Metais Pesados/isolamento & purificação
Níquel/metabolismo
Pseudomonas/isolamento & purificação
Pseudomonas putida/isolamento & purificação
Sideróforos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Industrial Waste); 0 (Metals, Heavy); 0 (Siderophores); 00BH33GNGH (Cadmium); 2P299V784P (Lead); 7OV03QG267 (Nickel); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


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[PMID]:28243718
[Au] Autor:Qiu J; Zhang J; Zhang Y; Wang Y; Tong L; Hong Q; He J
[Ad] Endereço:Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
[Ti] Título:Biodegradation of Picolinic Acid by a Newly Isolated Bacterium Alcaligenes faecalis Strain JQ135.
[So] Source:Curr Microbiol;74(4):508-514, 2017 Apr.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We isolated a bacterial strain JQ135 from municipal wastewater, which was capable of efficiently degrading picolinic acid (PA). Based on the physico-biochemical characteristics and 16S rDNA analysis, strain JQ135 was identified as Alcaligenes faecalis. In addition, strain JQ135 produced an orange pigment when cultured in the Luria-Bertani medium, which is different from the previously reported strains of A. faecalis. During the degradation of PA by the resting strain JQ135 cells, only one intermediate, 6-hydroxypicolinic acid (6HPA), was detected by ultraviolet spectrophotometry, high-pressure liquid chromatography, and liquid chromatography-mass spectrometry. A random transposon mutagenesis library of strain JQ135 was constructed. One mutant, Mut-G31, could convert PA into 6HPA without further degradation. The disrupted gene (orf2) was amplified from Mut-G31, and its product showed 32% identity to the 3-deoxy-D-manno-octulosonic acid kinase (KdkA) from Haemophilus influenzae. Results from complementation analysis confirmed that GTG was the initiation codon of the kdkA-like orf2, and that it was essential for PA biodegradation by strain JQ135. This study provides the first genetic evidence for the bacterial degradation of PA.
[Mh] Termos MeSH primário: Alcaligenes faecalis/metabolismo
Ácidos Picolínicos/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Cromatografia Líquida de Alta Pressão
Cromatografia Líquida
Elementos de DNA Transponíveis/genética
Espectrometria de Massas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (Picolinic Acids); QZV2W997JQ (picolinic acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-017-1205-2


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[PMID]:28217898
[Au] Autor:Matilda SC; Shanthi C
[Ad] Endereço:School of Bioscience and Technology, VIT University, Vellore-632014, India.
[Ti] Título:Metal induced changes in trivalent chromium resistant Alcaligenes faecalis VITSIM2.
[So] Source:J Basic Microbiol;57(5):402-412, 2017 May.
[Is] ISSN:1521-4028
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The changes induced in bacterial strains under stress conditions provide an insight into metal resistance strategies. Trivalent chromium resistant bacterium were isolated and identified by 16S rRNA gene sequencing and designated as Alcaligenes faecalis VITSIM2. The growth pattern was monitored. The organism also showed resistance to copper, cadmium, and certain antibiotics. The differentially expressed proteins in SDS PAGE were identified by mass spectrometry as flagellin and 50S ribosomal L36 protein. The morphological changes were identified by scanning electron microscopy. The changes in the cell wall content were estimated by peptidoglycan analysis and transformation of phosphates was detected by P NMR. Flow cytometry was employed to measure the membrane integrity, esterase activity and intracellular pH. In conclusion spectrum of proteomic, physiological, and morphological alterations was observed that aid the organism to overcome chromium stress.
[Mh] Termos MeSH primário: Alcaligenes faecalis/efeitos dos fármacos
Cromo/farmacologia
Metais/metabolismo
[Mh] Termos MeSH secundário: Alcaligenes faecalis/genética
Alcaligenes faecalis/isolamento & purificação
Alcaligenes faecalis/metabolismo
Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Membrana Celular/química
Membrana Celular/metabolismo
Parede Celular/metabolismo
DNA Bacteriano
Esterases/metabolismo
Genes Bacterianos
Peptidoglicano/metabolismo
Filogenia
RNA Ribossômico 16S/genética
Solo/química
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Metals); 0 (Peptidoglycan); 0 (RNA, Ribosomal, 16S); 0 (Soil); 0R0008Q3JB (Chromium); EC 3.1.- (Esterases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE
[do] DOI:10.1002/jobm.201600596


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[PMID]:27978438
[Au] Autor:Zhao B; Tian M; An Q; Ye J; Guo JS
[Ad] Endereço:Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, PR China; National Centre for International Research of Low-carbon and Green Buildings, Chongqing University, Chongqing 400045, PR China.
[Ti] Título:Characteristics of a heterotrophic nitrogen removal bacterium and its potential application on treatment of ammonium-rich wastewater.
[So] Source:Bioresour Technol;226:46-54, 2017 Feb.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nitrogen and organic carbon are major pollutants in wastewater causing environmental problems. Alcaligenes faecalis strain NR, isolated from activated sludge, exhibited the ability to remove ammonium and organic carbon from wastewater simultaneously under sole aerobic conditions in batch culture. Changes in carbon type, C/N ratio, oxygen concentration and inorganic ions significantly affected the treatment efficiency. Furthermore, a continuous bioreactor, solely inoculated with A. faecalis strain NR, was conducted to assess its feasibility for simultaneous nitrogen and organic matter removal in a single aerated reactor. Approximately 66.7-78.3% of NH -N and 85.8-92.2% of TOC were removed by using synthetic wastewater with 150-200mg/L of NH -N and 1350-2000mg/L of TOC. This research would be valuable to develop an innovative treatment method for ammonium-rich wastewater under aerobic conditions.
[Mh] Termos MeSH primário: Alcaligenes faecalis/metabolismo
Nitrogênio/metabolismo
Eliminação de Resíduos Líquidos/métodos
[Mh] Termos MeSH secundário: Aerobiose
Alcaligenes faecalis/isolamento & purificação
Compostos de Amônio/química
Compostos de Amônio/metabolismo
Reatores Biológicos/microbiologia
Carbono/metabolismo
Desnitrificação
Processos Heterotróficos
Esgotos/microbiologia
Eliminação de Resíduos Líquidos/instrumentação
Águas Residuais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Sewage); 0 (Waste Water); 7440-44-0 (Carbon); N762921K75 (Nitrogen)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE


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[PMID]:27816824
[Au] Autor:Ray S; Singh V; Singh S; Sarma BK; Singh HB
[Ad] Endereço:Department of Botany, Institute of Science, Banaras Hindu University, India.
[Ti] Título:Biochemical and histochemical analyses revealing endophytic Alcaligenes faecalis mediated suppression of oxidative stress in Abelmoschus esculentus challenged with Sclerotium rolfsii.
[So] Source:Plant Physiol Biochem;109:430-441, 2016 Dec.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Sclerotium rolfsii is a highly aggressive pathogen that causes huge economic losses, especially in temperate climates. Alcaligenes faecalis, particularly in endophytic form, has rarely been used to control this fungus. In this study, endophytic Alcaligenes sp. strain BHU 12, BHU 16 (isolated from Abelmoschus esculentus leaf) and BHU M7 (isolated from Andrographis paniculata leaf) were reported to trigger a wide range of host defenses in Okra plant against the collar-rot pathogen S. rolfsii. Endophytic colonization of the strains in ten days old plants was assessed through re-isolation of the rif-tagged strains on rifampicin augmented nutrient agar media. The ability of the endophytic strains to induce systemic defense responses in above-ground organs was assessed by collecting leaf tissues of the Okra plants grown under non-gnotobiotic conditions at different time intervals post seedling bacterization with the endophytic biocontrol agents. The pathogen challenged unprimed plants exhibited flaccidity of the stem and leaves at 48 h post infection (hpi) in contrast to the bioprimed and challenged plants. Biochemical and histochemical analyses explained the above phenomenon as activation of phyto-peroxidases leading to an increased metabolism of the reactive oxygen species (ROS), accompanied by activation of the phenylpropanoid network and a subsequent enhancement in plant phenolics. Interestingly, though the maximum increase in the defense pathways was observed in treatments with native endophytes of Okra plant, yet the enhancement in antioxidant pathway due to A. paniculata borne endophytes was also quite significant. Thus, this work clearly demonstrates how Okra plants respond to the "non-hostile" colonization of bacterial endophytes and how induced defense response can contribute to the biocontrol activity of the endophytic strains.
[Mh] Termos MeSH primário: Abelmoschus/metabolismo
Abelmoschus/microbiologia
Alcaligenes faecalis/metabolismo
Ascomicetos/patogenicidade
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: Abelmoschus/crescimento & desenvolvimento
Antioxidantes/metabolismo
Ácido Ascórbico/metabolismo
Morte Celular
Endófitos/metabolismo
Interações Hospedeiro-Patógeno
Peróxido de Hidrogênio/metabolismo
Lignina/metabolismo
Peroxidação de Lipídeos
Estresse Oxidativo
Doenças das Plantas/prevenção & controle
Folhas de Planta/citologia
Folhas de Planta/metabolismo
Folhas de Planta/microbiologia
Espécies Reativas de Oxigênio/metabolismo
Superóxidos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Reactive Oxygen Species); 11062-77-4 (Superoxides); 9005-53-2 (Lignin); BBX060AN9V (Hydrogen Peroxide); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:27591519
[Au] Autor:Li X; Xu QM; Cheng JS; Yuan YJ
[Ad] Endereço:Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center o
[Ti] Título:Improving the bioremoval of sulfamethoxazole and alleviating cytotoxicity of its biotransformation by laccase producing system under coculture of Pycnoporus sanguineus and Alcaligenes faecalis.
[So] Source:Bioresour Technol;220:333-340, 2016 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The occurrence of sulfamethoxazole (SMX) in aquatic environment is a health concern. The presence of SMX significantly inhibited the laccase activity of Pycnoporus sanguineus with a lower removal efficiency of SMX. Although a laccase system with 2,20-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) eliminated 100% SMX within 6h, ABTS might cause an environmental issue. An alternative to SMX elimination is the coculture of Alcaligenes faecalis and P. sanguineus. The SMX removal efficiency at 48h under the coculture with vitamins was higher than that under their pure culture alone, indicating that a coculture was more efficient in eliminating SMX than a pure culture. Only 1% SMX was detected in mycelia, indicating that SMX elimination is achieved primarily through biotransformation rather than adsorption. Laccase production by the coculture effectively inhibited the accumulations of N4-acetyl-SMX and N-hydroxy-SMX and alleviated the cytotoxicity of SMX transformation products. The mixture of SMX and sulfadiazine inhibited their removal efficiency.
[Mh] Termos MeSH primário: Alcaligenes faecalis/metabolismo
Pycnoporus/metabolismo
Sulfametoxazol/metabolismo
[Mh] Termos MeSH secundário: Adsorção
Biodegradação Ambiental
Técnicas de Cocultura
Lacase/metabolismo
Sulfametoxazol/análise
Sulfametoxazol/química
Trametes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.10.3.2 (Laccase); JE42381TNV (Sulfamethoxazole)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170923
[Lr] Data última revisão:
170923
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160904
[St] Status:MEDLINE


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[PMID]:27449326
[Au] Autor:Kim HS; Park KH; Lee HK; Kim JS; Kim YG; Lee JH; Kim KH; Yun J; Hwang BY; Hong JT; Kim Y; Han SB
[Ad] Endereço:College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
[Ti] Título:Curdlan activates dendritic cells through dectin-1 and toll-like receptor 4 signaling.
[So] Source:Int Immunopharmacol;39:71-78, 2016 Oct.
[Is] ISSN:1878-1705
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Curdlan, a ß-1,3-glucan isolated from Alcaligenes faecalis, is an agonist of dectin-1 in various immune cells, including dendritic cells (DCs). However, whether curdlan also activates DCs through other receptors remains unknown. In this study, we found that curdlan activates DCs through dectin-1 and toll-like receptor 4 (TLR4). Curdlan increased the expression levels of surface molecules (CD40, CD80, CD86, and MHC-I/II), the production of cytokines (IL-12, IL-1ß, TNF-α, and IFN-ß), migration toward MIP-3ß, and allogeneic T cell stimulation activity of DCs. Curdlan increased the phosphorylation of Syk, Raf-1, Akt, MAPKs, IKK, and NF-κB p65 in DCs. However, curdlan only slightly activated DCs transfected with small interfering RNAs against dectin-1 or TLR4 and C3H/HeJ DCs, which have non-functional TLR4, in comparison with control DCs. Curdlan increased antitumor activity of DCs in a syngeneic tumor model. In summary, our data show that curdlan activates DCs through dectin-1 and TLR4 signaling and the combination of curdlan and DCs efficiently inhibit tumor growth in mice.
[Mh] Termos MeSH primário: Alcaligenes faecalis/imunologia
Antineoplásicos/uso terapêutico
Células Dendríticas/efeitos dos fármacos
Lectinas Tipo C/metabolismo
Neoplasias Cutâneas/tratamento farmacológico
Linfócitos T/imunologia
Receptor 4 Toll-Like/metabolismo
beta-Glucanas/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/efeitos dos fármacos
Diferenciação Celular/genética
Movimento Celular/efeitos dos fármacos
Citocinas/metabolismo
Células Dendríticas/fisiologia
Mediadores da Inflamação/metabolismo
Lectinas Tipo C/genética
Ativação Linfocitária
Melanoma Experimental
Camundongos
Camundongos Endogâmicos C3H
Camundongos Endogâmicos C57BL
Camundongos Knockout
RNA Interferente Pequeno/genética
Receptor 4 Toll-Like/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Cytokines); 0 (Inflammation Mediators); 0 (Lectins, C-Type); 0 (RNA, Small Interfering); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 4); 0 (beta-Glucans); 0 (dectin 1); 6930DL209R (curdlan)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170813
[Lr] Data última revisão:
170813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE


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[PMID]:27391673
[Au] Autor:Mishra P; Kaur S; Sharma AN; Jolly RS
[Ad] Endereço:Department of Bioorganic Chemistry, CSIR-Institute of Microbial Technology, Sector 39, Chandigarh, India.
[Ti] Título:Characterization of an Indole-3-Acetamide Hydrolase from Alcaligenes faecalis subsp. parafaecalis and Its Application in Efficient Preparation of Both Enantiomers of Chiral Building Block 2,3-Dihydro-1,4-Benzodioxin-2-Carboxylic Acid.
[So] Source:PLoS One;11(7):e0159009, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Both the enantiomers of 2,3-dihydro-1,4-benzodioxin-2-carboxylic acid are valuable chiral synthons for enantiospecific synthesis of therapeutic agents such as (S)-doxazosin mesylate, WB 4101, MKC 242, 2,3-dihydro-2-hydroxymethyl-1,4-benzodioxin, and N-[2,4-oxo-1,3-thiazolidin-3-yl]-2,3-dihydro-1,4-benzodioxin-2-carboxamide. Pharmaceutical applications require these enantiomers in optically pure form. However, currently available methods suffer from one drawback or other, such as low efficiency, uncommon and not so easily accessible chiral resolving agent and less than optimal enantiomeric purity. Our interest in finding a biocatalyst for efficient production of enantiomerically pure 2,3-dihydro-1,4-benzodioxin-2-carboxylic acid lead us to discover an amidase activity from Alcaligenes faecalis subsp. parafaecalis, which was able to kinetically resolve 2,3-dihydro-1,4-benzodioxin-2-carboxyamide with E value of >200. Thus, at about 50% conversion, (R)-2,3-dihydro-1,4-benzodioxin-2-carboxylic acid was produced in >99% e.e. The remaining amide had (S)-configuration and 99% e.e. The amide and acid were easily separated by aqueous (alkaline)-organic two phase extraction method. The same amidase was able to catalyse, albeit at much lower rate the hydrolysis of (S)-amide to (S)-acid without loss of e.e. The amidase activity was identified as indole-3-acetamide hydrolase (IaaH). IaaH is known to catalyse conversion of indole-3-acetamide (IAM) to indole-3-acetic acid (IAA), which is phytohormone of auxin class and is widespread among plants and bacteria that inhabit plant rhizosphere. IaaH exhibited high activity for 2,3-dihydro-1,4-benzodioxin-2-carboxamide, which was about 65% compared to its natural substrate, indole-3-acetamide. The natural substrate for IaaH indole-3-acetamide shared, at least in part a similar bicyclic structure with 2,3-dihydro-1,4-benzodioxin-2-carboxamide, which may account for high activity of enzyme towards this un-natural substrate. To the best of our knowledge this is the first application of IaaH in production of industrially important molecules.
[Mh] Termos MeSH primário: Alcaligenes faecalis/enzimologia
Amidoidrolases/química
Proteínas de Bactérias/química
Ácidos Carboxílicos/química
Ácidos Carboxílicos/síntese química
[Mh] Termos MeSH secundário: Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Carboxylic Acids); EC 3.5.- (Amidohydrolases); EC 3.5.1.- (indole-3-acetamide hydrolase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0159009


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[PMID]:27060247
[Au] Autor:Wang Y; Chen H; Liu YX; Ren RP; Lv YK
[Ad] Endereço:Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China.
[Ti] Título:An adsorption-release-biodegradation system for simultaneous biodegradation of phenol and ammonium in phenol-rich wastewater.
[So] Source:Bioresour Technol;211:711-9, 2016 Jul.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The feasibility of simultaneous biodegradation of phenol and ammonium in phenol-rich wastewater was evaluated in a reusable system, which contained macroporous adsorption resin and Alcaligenes faecalis strain WY-01. In the system, up to 6000mg/L phenol could be completely degraded by WY-01; meanwhile, 99.03±3.95% of ammonium was removed from the initial concentration of 384mg/L. This is the first study to show the capability of single strain in simultaneous removal of ammonium and phenol in wastewater containing such high concentrations of phenol. Moreover, the resin was regenerated during the biodegradation process without any additional manipulations, indicating the system was reusable. Furthermore, enzyme assay, gene expression patterns, HPLC-MS and gas chromatography analysis confirmed that phenol biodegradation accompanied with aerobic nitrifier denitrification process. Results imply that the reusable system provides a novel strategy for more efficient biodegradation of phenol and ammonium contained in some particular industrial wastewater.
[Mh] Termos MeSH primário: Compostos de Amônio/metabolismo
Fenol/metabolismo
Águas Residuais/química
[Mh] Termos MeSH secundário: Adsorção
Aerobiose
Alcaligenes faecalis/genética
Alcaligenes faecalis/metabolismo
Biodegradação Ambiental
Regulação Bacteriana da Expressão Gênica
Concentração de Íons de Hidrogênio
Nitrogênio/análise
Porosidade
Resinas Sintéticas/química
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Resins, Synthetic); 0 (Waste Water); 339NCG44TV (Phenol); N762921K75 (Nitrogen)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160410
[St] Status:MEDLINE



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