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[PMID]:28632823
[Au] Autor:Giayetto VO; Blanco S; Mangeaud A; Barbás MG; Cudolá A; Gallego SV
[Ad] Endereço:Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
[Ti] Título:[Features of Bordetella pertussis, Bordetella spp. infection and whopping cough in Córdoba province, Argentina].
[Ti] Título:Caracterización de la infección por Bordetella pertussis, Bordetella spp. y coqueluche en la provincia de Córdoba, Argentina..
[So] Source:Rev Chilena Infectol;34(2):108-115, 2017 Apr.
[Is] ISSN:0717-6341
[Cp] País de publicação:Chile
[La] Idioma:spa
[Ab] Resumo:INTRODUCTION: Whooping cough is a re-emerging infection in the world and Latin America. OBJECTIVE: It was considered relevant to investigate the clinical and epidemiological profile of Bordetella spp. and Bordetella pertussis infection in Córdoba province, Argentina; evaluating, at the same time, the co-infection with virus producing respiratory infections that may be confused with whooping cough. MATERIAL AND METHODS: All whooping cough suspected cases were studied by Polimerase Chain Reaction, amplifying the repeated insertion sequence (IS) 481 and the promoter gene encoding pertussis toxin, between 2011 and 2013. The data were obtained from the clinical and epidemiological records. RESULTS: From 2,588 whooping cough suspected cases, 11.59% was infected by Bordetella spp. and 9.16% was confirmed as Bordetella pertussis infection. The rate of infection was 7.22 and 1.84 per 100,000 for 2011 and 2012, respectively. The infection presented a seasonal tendency and it was mainly found on the group of children between 13 and 24 months old. The co-infection with virus producing respiratory infections, were uncommon. Paroxysmal cough, cyanosis and/or vomiting were predictors of the infection for Bordetella pertussis. DISCUSSION AND CONCLUSIONS: To deal with the re-emergence of whooping cough is important the knowledge of the regional epidemiological situation. This paper shows the situation of these infections in the regional clinical and epidemiological context, and makes the information available for health decision-making.
[Mh] Termos MeSH primário: Bordetella/genética
Doenças Transmissíveis Emergentes/epidemiologia
Coqueluche/diagnóstico
[Mh] Termos MeSH secundário: Argentina/epidemiologia
Bordetella/classificação
Bordetella pertussis/genética
Criança
Pré-Escolar
Doenças Transmissíveis Emergentes/diagnóstico
Doenças Transmissíveis Emergentes/virologia
Diagnóstico Diferencial
Feminino
Seres Humanos
Lactente
Masculino
Reação em Cadeia da Polimerase
Coqueluche/epidemiologia
Coqueluche/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE


  2 / 1072 MEDLINE  
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[PMID]:27783449
[Au] Autor:Brickman TJ; Suhadolc RJ; McKelvey PJ; Armstrong SK
[Ad] Endereço:Department of Microbiology and Immunology, University of Minnesota Medical School, 3-117 Microbiology Research Facility, 689 23rd Ave. S.E, Minneapolis, MN, 55455-1507, USA.
[Ti] Título:Essential role of Bordetella NadC in a quinolinate salvage pathway for NAD biosynthesis.
[So] Source:Mol Microbiol;103(3):423-438, 2017 Feb.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nicotinamide adenine dinucleotide (NAD) is produced via de novo biosynthesis pathways and by salvage or recycling routes. The classical Bordetella bacterial species are known to be auxotrophic for nicotinamide or nicotinic acid. This study confirmed that Bordetella bronchiseptica, Bordetella pertussis and Bordetella parapertussis have the recycling/salvage pathway genes pncA and pncB, for use of nicotinamide or nicotinic acid, respectively, for NAD synthesis. Although these Bordetellae lack the nadA and nadB genes needed for de novo NAD biosynthesis, remarkably, they have one de novo pathway gene, nadC, encoding quinolinate phosphoribosyltransferase. Genomic analyses of taxonomically related Bordetella and Achromobacter species also indicated the presence of an 'orphan' nadC and the absence of nadA and nadB. When supplied as the sole NAD precursor, quinolinate promoted B. bronchiseptica growth, and the ability to use it required nadC. Co-expression of Bordetella nadC with the nadB and nadA genes of Paraburkholderia phytofirmans allowed B. bronchiseptica to grow in the absence of supplied pyridines, indicative of de novo NAD synthesis and functional confirmation of Bordetella NadC activity. Expression of nadC in B. bronchiseptica was influenced by nicotinic acid and by a NadQ family transcriptional repressor, indicating that these organisms prioritize their use of pyridines for NAD biosynthesis.
[Mh] Termos MeSH primário: NAD/biossíntese
Pentosiltransferases/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Vias Biossintéticas
Bordetella/genética
Genes Bacterianos/genética
Mutação
Ácido Quinolínico/metabolismo
Ácido Quinolínico/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0U46U6E8UK (NAD); EC 2.4.2.- (Pentosyltransferases); EC 2.4.2.19 (nicotinate-nucleotide diphosphorylase (carboxylating)); EC 6.3.4.21 (nicotinate phosphoribosyltransferase); F6F0HK1URN (Quinolinic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161027
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13566


  3 / 1072 MEDLINE  
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[PMID]:27716057
[Au] Autor:Linz B; Ivanov YV; Preston A; Brinkac L; Parkhill J; Kim M; Harris SR; Goodfield LL; Fry NK; Gorringe AR; Nicholson TL; Register KB; Losada L; Harvill ET
[Ad] Endereço:Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA 16802, USA. bxl29@psu.edu.
[Ti] Título:Acquisition and loss of virulence-associated factors during genome evolution and speciation in three clades of Bordetella species.
[So] Source:BMC Genomics;17(1):767, 2016 09 30.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The genus Bordetella consists of nine species that include important respiratory pathogens such as the 'classical' species B. bronchiseptica, B. pertussis and B. parapertussis and six more distantly related and less extensively studied species. Here we analyze sequence diversity and gene content of 128 genome sequences from all nine species with focus on the evolution of virulence-associated factors. RESULTS: Both genome-wide sequence-based and gene content-based phylogenetic trees divide the genus into three species clades. The phylogenies are congruent between species suggesting genus-wide co-evolution of sequence diversity and gene content, but less correlated within species, mainly because of strain-specific presence of many different prophages. We compared the genomes with focus on virulence-associated genes and identified multiple clade-specific, species-specific and strain-specific events of gene acquisition and gene loss, including genes encoding O-antigens, protein secretion systems and bacterial toxins. Gene loss was more frequent than gene gain throughout the evolution, and loss of hundreds of genes was associated with the origin of several species, including the recently evolved human-restricted B. pertussis and B. holmesii, B. parapertussis and the avian pathogen B. avium. CONCLUSIONS: Acquisition and loss of multiple genes drive the evolution and speciation in the genus Bordetella, including large scale gene loss associated with the origin of several species. Recent loss and functional inactivation of genes, including those encoding pertussis vaccine components and bacterial toxins, in individual strains emphasize ongoing evolution.
[Mh] Termos MeSH primário: Bordetella/classificação
Bordetella/genética
Evolução Molecular
Genoma Bacteriano
Fatores de Virulência/genética
[Mh] Termos MeSH secundário: Animais
Sistemas de Secreção Bacterianos/genética
Infecções por Bordetella/microbiologia
Conjuntos de Dados como Assunto
Genes Bacterianos
Variação Genética
Genômica
Genótipo
Seres Humanos
Tipagem de Sequências Multilocus
Filogenia
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Bacterial Secretion Systems); 0 (Virulence Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161008
[St] Status:MEDLINE


  4 / 1072 MEDLINE  
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[PMID]:27707434
[Au] Autor:Ivanov YV; Linz B; Register KB; Newman JD; Taylor DL; Boschert KR; Le Guyon S; Wilson EF; Brinkac LM; Sanka R; Greco SC; Klender PM; Losada L; Harvill ET
[Ad] Endereço:1​Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA.
[Ti] Título:Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice.
[So] Source:Int J Syst Evol Microbiol;66(12):5452-5459, 2016 Dec.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii-like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii, one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA-DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii - from poultry, humans and rabbit - and Bordetella pseudohinzii sp. nov. type strain 8-296-03T (=NRRL B-59942T=NCTC 13808T) that infect mice.
[Mh] Termos MeSH primário: Bordetella/classificação
Camundongos Endogâmicos C57BL/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
Bordetella/genética
Bordetella/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/análise
Seres Humanos
Camundongos
Hibridização de Ácido Nucleico
Aves Domésticas
RNA Ribossômico 16S/genética
Coelhos
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161007
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001540


  5 / 1072 MEDLINE  
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[PMID]:27515393
[Au] Autor:Saito M; Odanaka K; Otsuka N; Kamachi K; Watanabe M
[Ad] Endereço:Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
[Ti] Título:Development of vaccines against pertussis caused by Bordetella holmesii using a mouse intranasal challenge model.
[So] Source:Microbiol Immunol;60(9):599-608, 2016 Sep.
[Is] ISSN:1348-0421
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Bordetella holmesii is recognized as the third causative agent of pertussis (whooping cough) in addition to Bordetella pertussis and Bordetella parapertussis. Pertussis caused by B. holmesii is not rare around the world. However, to date, there is no effective vaccine against B. holmesii. We examined the protective potency of pertussis vaccines available in Japan and vaccines prepared from B. holmesii. A murine model of respiratory infection was exploited to evaluate protective potency. No Japanese commercial pertussis vaccines were effective against B. holmesii. In contrast, a wBH vaccine and an aBH vaccine prepared from B. holmesii were both protective. Passive immunization with sera from mice immunized with aBH vaccine established protection against B. holmesii, indicating that B. holmesii-specific serum antibodies might play an important role in protection. Immuno-proteomic analysis with sera from mice immunized with aBH vaccine revealed that the sera recognized a BipA-like protein of B. holmesii. An aBH vaccine prepared from a BipA-like protein-deficient mutant strain did not have a protective effect against B. holmesii. Taken together, our results suggest that the BipA-like protein plays an important role in the protective efficacy of aBH vaccine.
[Mh] Termos MeSH primário: Bordetella pertussis/imunologia
Bordetella/imunologia
Reações Cruzadas/imunologia
Vacina contra Coqueluche/imunologia
Coqueluche/imunologia
Coqueluche/prevenção & controle
[Mh] Termos MeSH secundário: Administração Intranasal
Transferência Adotiva
Animais
Anticorpos Antibacterianos/sangue
Anticorpos Antibacterianos/imunologia
Antígenos de Bactérias/imunologia
Proteínas da Membrana Bacteriana Externa/genética
Proteínas da Membrana Bacteriana Externa/imunologia
Bordetella/genética
Bordetella pertussis/genética
Modelos Animais de Doenças
Mapeamento de Epitopos
Feminino
Soros Imunes/administração & dosagem
Imunização
Camundongos
Vacina contra Coqueluche/administração & dosagem
Baço/citologia
Baço/imunologia
Coqueluche/mortalidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Bacterial Outer Membrane Proteins); 0 (BipA protein, Bordetella); 0 (Immune Sera); 0 (Pertussis Vaccine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160813
[St] Status:MEDLINE
[do] DOI:10.1111/1348-0421.12409


  6 / 1072 MEDLINE  
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[PMID]:27448237
[Au] Autor:Hiramatsu Y; Saito M; Otsuka N; Suzuki E; Watanabe M; Shibayama K; Kamachi K
[Ad] Endereço:Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
[Ti] Título:BipA Is Associated with Preventing Autoagglutination and Promoting Biofilm Formation in Bordetella holmesii.
[So] Source:PLoS One;11(7):e0159999, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bordetella holmesii causes both invasive and respiratory diseases in humans. Although the number of cases of pertussis-like respiratory illnesses due to B. holmesii infection has increased in the last decade worldwide, little is known about the virulence factors of the organism. Here, we analyzed a B. holmesii isolate that forms large aggregates and precipitates in suspension, and subsequently demonstrated that the autoagglutinating isolate is deficient in Bordetella intermediate protein A (BipA) and that this deletion is caused by a frame-shift mutation in the bipA gene. A BipA-deficient mutant generated by homologous recombination also exhibited the autoagglutination phenotype. Moreover, the BipA mutant adhered poorly to an abiotic surface and failed to form biofilms, as did two other B. holmesii autoagglutinating strains, ATCC 51541 and ATCC 700053, which exhibit transcriptional down-regulation of bipA gene expression, indicating that autoagglutination indirectly inhibits biofilm formation. In a mouse intranasal infection model, the BipA mutant showed significantly lower levels of initial lung colonization than did the parental strain (P < 0.01), suggesting that BipA might be a critical virulence factor in B. holmesii respiratory infection. Together, our findings suggest that BipA production plays an essential role in preventing autoagglutination and indirectly promoting biofilm formation by B. holmesii.
[Mh] Termos MeSH primário: Aglutinação/genética
Proteínas da Membrana Bacteriana Externa/genética
Proteínas da Membrana Bacteriana Externa/metabolismo
Biofilmes
Bordetella/fisiologia
[Mh] Termos MeSH secundário: Testes de Aglutinação
Sequência de Aminoácidos
Animais
Proteínas da Membrana Bacteriana Externa/química
Infecções por Bordetella/microbiologia
Regulação Bacteriana da Expressão Gênica
Camundongos
Mutação
Pneumonia Bacteriana/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (BipA protein, Bordetella)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0159999


  7 / 1072 MEDLINE  
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[PMID]:27397578
[Au] Autor:Majewski LL; Nogi M; Bankowski MJ; Chung HH
[Ad] Endereço:Department of Medicine, John A. Burns School of Medicine, University of Hawaii at Manoa, 1356 Lusitana Street, 7th floor, Honolulu, HI, 96813, USA. Electronic address: lorrance@hawaii.edu.
[Ti] Título:Bordetella trematum sepsis with shock in a diabetic patient with rapidly developing soft tissue infection.
[So] Source:Diagn Microbiol Infect Dis;86(1):112-4, 2016 Sep.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bordetella is a gram-negative, glucose non-fermenting bacillus, consisting of many host-associated species. B. trematum has previously been identified in wound infections, but rarely known to be a source of bacteremia. Currently, 16S rRNA sequencing represents the reference standard method by which identification is made. Herein, we present a case of fatal B. trematum bacteremia with septic shock. The presumed primary site of the infection was a rapidly developing left leg deep soft tissue infection without necrotizing fasciitis. B. trematum should now be considered as a significant pathogen in sepsis.
[Mh] Termos MeSH primário: Infecções por Bordetella/diagnóstico
Infecções por Bordetella/patologia
Bordetella/isolamento & purificação
Choque Séptico/diagnóstico
Choque Séptico/patologia
Infecções dos Tecidos Moles/complicações
Infecções dos Tecidos Moles/diagnóstico
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Bordetella/classificação
Bordetella/efeitos dos fármacos
Bordetella/genética
Infecções por Bordetella/microbiologia
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Seres Humanos
Perna (Membro)/patologia
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Choque Séptico/microbiologia
Infecções dos Tecidos Moles/microbiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE


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[PMID]:27227292
[Au] Autor:Pittet LF; Posfay-Barbe KM
[Ti] Título:Bordetella holmesii: Still Emerging and Elusive 20 Years On.
[So] Source:Microbiol Spectr;4(2), 2016 Apr.
[Is] ISSN:2165-0497
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Since the first description of Bordetella holmesii in 1995, almost 100 publications have contributed to the increasing knowledge of this emerging bacterium. Although first reported to induce bacteremia mainly in immunocompromised patients, it has also been isolated in healthy persons and has shown the capacity to induce pertussis-like symptoms and other clinical entities, such as meningitis, arthritis, or endocarditis. Respiratory diseases are generally less severe than those induced by Bordetella pertussis. However, B. holmesii was found to have a higher capacity of invasiveness given the various infection sites in which it was isolated. The diagnosis is difficult, particularly as it is a slow-growing organism but also because respiratory infections are systematically misdiagnosed as B. pertussis. Treatment is delicate, as its susceptibility to macrolides (prescribed in respiratory infections) and ceftriaxone (used in invasive disease) is challenged. Regarding prevention, there is no consensus on prophylactic treatment following index cases and no vaccine is available. Epidemiological data are also sparse, with few prevalence studies available. In this chapter, we provide an overview of the current state of knowledge on B. holmesii.
[Mh] Termos MeSH primário: Infecções por Bordetella/microbiologia
Bordetella/fisiologia
[Mh] Termos MeSH secundário: Bordetella/efeitos dos fármacos
Bordetella/patogenicidade
Infecções por Bordetella/diagnóstico
Infecções por Bordetella/epidemiologia
Infecções por Bordetella/terapia
Ceftriaxona/uso terapêutico
Seres Humanos
Macrolídeos/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Macrolides); 75J73V1629 (Ceftriaxone)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160527
[St] Status:MEDLINE
[do] DOI:10.1128/microbiolspec.EI10-0003-2015


  9 / 1072 MEDLINE  
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[PMID]:27188224
[Au] Autor:Blanco A; Girón RM; Sáez-Nieto JA; Alarcón T
[Ad] Endereço:Ana Blanco Suárez, Servicio de Microbiología y Parasitología Hospital Universitario La Princesa, C/Diego de León, 62, CP. 28006, Madrid, Spain.
[Ti] Título:[Bordetella petrii chronic colonization. First case in Spain].
[Ti] Título:Colonización crónica por Bordetella petrii. Primer caso en España..
[So] Source:Rev Esp Quimioter;29(3):167-9, 2016 Jun.
[Is] ISSN:1988-9518
[Cp] País de publicação:Spain
[La] Idioma:spa
[Mh] Termos MeSH primário: Infecções por Bordetella/microbiologia
Bordetella
[Mh] Termos MeSH secundário: Idoso
Antibacterianos/farmacologia
Antibacterianos/uso terapêutico
Bordetella/efeitos dos fármacos
Infecções por Bordetella/tratamento farmacológico
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana
RNA Bacteriano
RNA Ribossômico 16S
Espanha
[Pt] Tipo de publicação:CASE REPORTS; LETTER
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160519
[St] Status:MEDLINE


  10 / 1072 MEDLINE  
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[PMID]:27075457
[Au] Autor:Liu Y; Liu P; Lin L; Zhao Y; Zhong W; Wu L; Zhou Z; Sun W
[Ad] Endereço:Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, 610039, China.
[Ti] Título:Activity Enhancement Based on the Chemical Equilibrium of Multiple-Subunit Nitrile Hydratase from Bordetella petrii.
[So] Source:Appl Biochem Biotechnol;180(1):3-9, 2016 Sep.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The maturation mechanism of nitrile hydratase (NHase) of Pseudomonas putida NRRL-18668 was discovered and named as "self-subunit swapping." Since the NHase of Bordetella petrii DSM 12804 is similar to that of P. putida, the NHase maturation of B. petrii is proposed to be the same as that of P. putida. However, there is no further information on the application of NHase according to these findings. We successfully rapidly purified NHase and its activator through affinity his tag, and found that the cell extracts of NHase possessed multiple types of protein ingredients including α, ß, α2ß2, and α(P14K)2 who were in a state of chemical equilibrium. Furthermore, the activity was significantly enhanced through adding extra α(P14K)2 to the cell extracts of NHase according to the chemical equilibrium. Our findings are useful for the activity enhancement of multiple-subunit enzyme and for the first time significantly increased the NHase activity according to the chemical equilibrium.
[Mh] Termos MeSH primário: Bordetella/enzimologia
Hidroliases/metabolismo
Subunidades Proteicas/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Cromatografia de Afinidade
Eletroforese em Gel de Poliacrilamida
Hidroliases/isolamento & purificação
Cinética
Modelos Biológicos
Plasmídeos/metabolismo
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Protein Subunits); 0 (Recombinant Proteins); EC 4.2.1.- (Hydro-Lyases); EC 4.2.1.- (nitrile hydratase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160415
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-016-2079-7



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