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Pesquisa : B03.440.400.425.180.500 [Categoria DeCS]
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  1 / 11 MEDLINE  
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[PMID]:28710792
[Au] Autor:Korasick DA; Singh H; Pemberton TA; Luo M; Dhatwalia R; Tanner JJ
[Ad] Endereço:Department of Biochemistry, University of Missouri, Columbia, MO, USA.
[Ti] Título:Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.
[So] Source:FEBS J;284(18):3029-3049, 2017 Sep.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many enzymes form homooligomers, yet the functional significance of self-association is seldom obvious. Herein, we examine the connection between oligomerization and catalytic function for proline utilization A (PutA) enzymes. PutAs are bifunctional enzymes that catalyze both reactions of proline catabolism. Type A PutAs are the smallest members of the family, possessing a minimal domain architecture consisting of N-terminal proline dehydrogenase and C-terminal l-glutamate-γ-semialdehyde dehydrogenase modules. Type A PutAs form domain-swapped dimers, and in one case (Bradyrhizobium japonicum PutA), two of the dimers assemble into a ring-shaped tetramer. Whereas the dimer has a clear role in substrate channeling, the functional significance of the tetramer is unknown. To address this question, we performed structural studies of four-type A PutAs from two clades of the PutA tree. The crystal structure of Bdellovibrio bacteriovorus PutA covalently inactivated by N-propargylglycine revealed a fold and substrate-channeling tunnel similar to other PutAs. Small-angle X-ray scattering (SAXS) and analytical ultracentrifugation indicated that Bdellovibrio PutA is dimeric in solution, in contrast to the prediction from crystal packing of a stable tetrameric assembly. SAXS studies of two other type A PutAs from separate clades also suggested that the dimer predominates in solution. To assess whether the tetramer of B. japonicum PutA is necessary for catalytic function, a hot spot disruption mutant that cleanly produces dimeric protein was generated. The dimeric variant exhibited kinetic parameters similar to the wild-type enzyme. These results implicate the domain-swapped dimer as the core structural and functional unit of type A PutAs. ENZYMES: Proline dehydrogenase (EC 1.5.5.2); l-glutamate-γ-semialdehyde dehydrogenase (EC 1.2.1.88). DATABASES: The atomic coordinates and structure factor amplitudes have been deposited in the Protein Data Bank under accession number 5UR2. The SAXS data have been deposited in the SASBDB under the following accession codes: SASDCP3 (BbPutA), SASDCQ3 (DvPutA 1.5 mg·mL ), SASDCX3 (DvPutA 3.0 mg·mL ), SASDCY3 (DvPutA 4.5 mg·mL ), SASDCR3 (LpPutA 3.0 mg·mL ), SASDCV3 (LpPutA 5.0 mg·mL ), SASDCW3 (LpPutA 8.0 mg·mL ), SASDCS3 (BjPutA 2.3 mg·mL ), SASDCT3 (BjPutA 4.7 mg·mL ), SASDCU3 (BjPutA 7.0 mg·mL ), SASDCZ3 (R51E 2.3 mg·mL ), SASDC24 (R51E 4.7 mg·mL ), SASDC34 (R51E 7.0 mg·mL ).
[Mh] Termos MeSH primário: Alquinos/química
Proteínas de Bactérias/química
Bdellovibrio bacteriovorus/química
Bradyrhizobium/química
Glicina/análogos & derivados
Proteínas de Membrana/química
Prolina/química
[Mh] Termos MeSH secundário: Alquinos/metabolismo
Motivos de Aminoácidos
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Bdellovibrio bacteriovorus/enzimologia
Sítios de Ligação
Bradyrhizobium/enzimologia
Clonagem Molecular
Cristalografia por Raios X
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Glicina/química
Glicina/metabolismo
Cinética
Proteínas de Membrana/genética
Proteínas de Membrana/metabolismo
Modelos Moleculares
Filogenia
Prolina/metabolismo
Ligação Proteica
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Multimerização Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Espalhamento a Baixo Ângulo
Homologia Estrutural de Proteína
Especificidade por Substrato
Termodinâmica
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkynes); 0 (Bacterial Proteins); 0 (Membrane Proteins); 0 (PutA protein, Bacteria); 0 (Recombinant Proteins); 64165-64-6 (propargylglycine); 9DLQ4CIU6V (Proline); TE7660XO1C (Glycine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170716
[St] Status:MEDLINE
[do] DOI:10.1111/febs.14165


  2 / 11 MEDLINE  
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[PMID]:28355554
[Au] Autor:Jashnsaz H; Al Juboori M; Weistuch C; Miller N; Nguyen T; Meyerhoff V; McCoy B; Perkins S; Wallgren R; Ray BD; Tsekouras K; Anderson GG; Pressé S
[Ad] Endereço:Department of Physics, Indiana University - Purdue University Indianapolis (IUPUI), Indianapolis, Indiana.
[Ti] Título:Hydrodynamic Hunters.
[So] Source:Biophys J;112(6):1282-1289, 2017 Mar 28.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Gram-negative Bdellovibrio bacteriovorus (BV) is a model bacterial predator that hunts other bacteria and may serve as a living antibiotic. Despite over 50 years since its discovery, it is suggested that BV probably collides into its prey at random. It remains unclear to what degree, if any, BV uses chemical cues to target its prey. The targeted search problem by the predator for its prey in three dimensions is a difficult problem: it requires the predator to sensitively detect prey and forecast its mobile prey's future position on the basis of previously detected signal. Here instead we find that rather than chemically detecting prey, hydrodynamics forces BV into regions high in prey density, thereby improving its odds of a chance collision with prey and ultimately reducing BV's search space for prey. We do so by showing that BV's dynamics are strongly influenced by self-generated hydrodynamic flow fields forcing BV onto surfaces and, for large enough defects on surfaces, forcing BV in orbital motion around these defects. Key experimental controls and calculations recapitulate the hydrodynamic origin of these behaviors. While BV's prey (Escherichia coli) are too small to trap BV in hydrodynamic orbit, the prey are also susceptible to their own hydrodynamic fields, substantially confining them to surfaces and defects where mobile predator and prey density is now dramatically enhanced. Colocalization, driven by hydrodynamics, ultimately reduces BV's search space for prey from three to two dimensions (on surfaces) even down to a single dimension (around defects). We conclude that BV's search for individual prey remains random, as suggested in the literature, but confined, however-by generic hydrodynamic forces-to reduced dimensionality.
[Mh] Termos MeSH primário: Bdellovibrio bacteriovorus/fisiologia
Hidrodinâmica
[Mh] Termos MeSH secundário: Escherichia coli/fisiologia
Processos Estocásticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE


  3 / 11 MEDLINE  
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[PMID]:28334102
[Au] Autor:Feng S; Tan CH; Constancias F; Kohli GS; Cohen Y; Rice SA
[Ad] Endereço:The Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore.
[Ti] Título:Predation by Bdellovibrio bacteriovorus significantly reduces viability and alters the microbial community composition of activated sludge flocs and granules.
[So] Source:FEMS Microbiol Ecol;93(4), 2017 Apr 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We recently isolated and characterised a predatory Bdellovibrio bacteriovorus strain from activated sludge (Ulu Pandan Water Reclamation Plant, Singapore), and this strain, B. bacteriovorus UP, was able to prey upon a broad spectrum of bacterial isolates from the activated sludge when grown as planktonic cells or as biofilms. Here, we have tested the effect of Bdellovibrio predation on floccular and granular sludge to determine if the spatial organisation, loosely or tightly aggregated communities, was protective from predation. The effect of predation was assessed using a combination of biomass quantification, cellular activity measurement and microscopic image analysis to determine community viability. Additionally, changes in the microbial communities due to predation by B. bacteriovorus UP were analysed through total RNA sequencing. Predation led to a significant reduction in microbial activity and total biomass for both floccular and granular sludge communities. Predation was also associated with significant changes in the microbial community composition in both communities, with >90% of the community members reduced in relative abundance after 24 h. Of those community members, the dominant organisms, such as Proteobacteria and Bacteroidetes, were the most affected phylotypes. This suggests that predatory bacteria, which display indiscriminant feeding, could significantly shift the species composition and thus, may disturb the operational performance of wastewater treatment systems.
[Mh] Termos MeSH primário: Bdellovibrio bacteriovorus/fisiologia
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Bactérias
Bdellovibrio/genética
Biofilmes
Singapura
Eliminação de Resíduos Líquidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sewage)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix020


  4 / 11 MEDLINE  
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[PMID]:28177793
[Au] Autor:McNeely D; Chanyi RM; Dooley JS; Moore JE; Koval SF
[Ad] Endereço:a Department of Microbiology and Immunology, University of Western Ontario, London, ON N6A 5C1, Canada.
[Ti] Título:Biocontrol of Burkholderia cepacia complex bacteria and bacterial phytopathogens by Bdellovibrio bacteriovorus.
[So] Source:Can J Microbiol;63(4):350-358, 2017 Apr.
[Is] ISSN:1480-3275
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Bdellovibrio and like organisms are predatory bacteria that have the unusual property of using the cytoplasmic constituents of other Gram-negative bacteria as nutrients. These predators may thus provide an alternative approach to the biocontrol of human and plant pathogens. Predators were isolated on Burkholderia cenocepacia K56-2 and J2315 as prey cells, in enrichment cultures with soil and sewage. Three isolates (DM7C, DM8A, and DM11A) were identified as Bdellovibrio bacteriovorus on the basis of morphology, a periplasmic life cycle, and 16S rRNA gene sequencing. The prey range of these isolates was tested on Burkholderia cepacia complex bacteria and several phytopathogenic bacteria of agricultural importance. Of 31 strains of the Burkholderia cepacia complex tested, only 4 were resistant to predation by strain DM7C. A subset of 9 of the prey tested were also susceptible to strains DM8A and DM11A. Of 12 phytopathogens tested, 4 were resistant to strains DM7C and DM8A, and only 2 were resistant to strain DM11A. Thus, Bdellovibrio bacteriovorus strains retrieved from environmental samples on 2 Burkholderia cenocepacia isolates from cystic fibrosis patients did not distinguish in their prey range between other isolates of that pathogen or phytopathogens. Such strains hold promise as potential wide-spectrum biocontrol agents.
[Mh] Termos MeSH primário: Bdellovibrio bacteriovorus/fisiologia
Complexo Burkholderia cepacia/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Antibiose
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1139/cjm-2016-0612


  5 / 11 MEDLINE  
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[PMID]:28053112
[Au] Autor:Dattner I; Miller E; Petrenko M; Kadouri DE; Jurkevitch E; Huppert A
[Ad] Endereço:Department of Statistics, University of Haifa, 199 Abba Khoushy Avenue, Mount Carmel, Haifa 3498838, Israel idattner@stat.haifa.ac.il.
[Ti] Título:Modelling and parameter inference of predator-prey dynamics in heterogeneous environments using the direct integral approach.
[So] Source:J R Soc Interface;14(126), 2017 Jan.
[Is] ISSN:1742-5662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Most bacterial habitats are topographically complex in the micro scale. Important examples include the gastrointestinal and tracheal tracts, and the soil. Although there are myriad theoretical studies that explore the role of spatial structures on antagonistic interactions (predation, competition) among animals, there are many fewer experimental studies that have explored, validated and quantified their predictions. In this study, we experimentally monitored the temporal dynamic of the predatory bacterium Bdellovibrio bacteriovorus, and its prey, the bacterium Burkholderia stabilis in a structured habitat consisting of sand under various regimes of wetness. We constructed a dynamic model, and estimated its parameters by further developing the direct integral method, a novel estimation procedure that exploits the separability of the states and parameters in the model. We also verified that one of our parameter estimates was consistent with its known, directly measured value from the literature. The ability of the model to fit the data combined with realistic parameter estimates indicate that bacterial predation in the sand can be described by a relatively simple model, and stress the importance of prey refuge on predation dynamics in heterogeneous environments.
[Mh] Termos MeSH primário: Bdellovibrio bacteriovorus/fisiologia
Burkholderia/fisiologia
Cadeia Alimentar
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE


  6 / 11 MEDLINE  
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[PMID]:27824344
[Au] Autor:Otto S; Bruni EP; Harms H; Wick LY
[Ad] Endereço:Department of Environmental Microbiology, UFZ-Helmholtz Centre for Environmental Research, Leipzig, Germany.
[Ti] Título:Catch me if you can: dispersal and foraging of Bdellovibrio bacteriovorus 109J along mycelia.
[So] Source:ISME J;11(2):386-393, 2017 Feb.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To cope with heterogeneous environments and resource distributions, filamentous fungi have evolved a spatially extensive growth enabling their hyphae to penetrate air-water interfaces and pass through air-filled pores. Such mycelia are also known to act as dispersal networks for the mobilisation of bacteria ('fungal highways') and connection of microbial microhabitats. Hitherto, however, nothing is known about the effect of mycelia-based dispersal on interactions between bacterial predators and their prey and concomitant effects on biomass formation. We here hypothesise that mycelia enable the contact between predators and their prey and shape a prey's population. We investigated the impact of predation by Bdellovibrio bacteriovorus 109J on the growth of its potential prey Pseudomonas fluorescens LP6a in the presence of mycelia. Our data give evidence that hyphae increase the accessibility of the prey to B. bacteriovorus 109J and, hence, allow for efficient foraging and shaping of prey populations not seen in the absence of mycelia. To test our hypothesis tailored microbial landscapes were used for better reduction of emerging properties in complex systems. Our data suggest that mycelia have substantial influence on prey-predator relationship and hereby may promote the structure of prey and predator populations and, hence, may be a determinant for biomass formation in heterogeneous environments.
[Mh] Termos MeSH primário: Bdellovibrio bacteriovorus/fisiologia
Fungos/fisiologia
Interações Microbianas
Micélio
Pseudomonas fluorescens/fisiologia
[Mh] Termos MeSH secundário: Bdellovibrio bacteriovorus/crescimento & desenvolvimento
Biomassa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2016.135


  7 / 11 MEDLINE  
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[PMID]:27580114
[Au] Autor:Boileau MJ; Mani R; Breshears MA; Gilmour M; Taylor JD; Clinkenbeard KD
[Ti] Título:Efficacy of Bdellovibrio bacteriovorus 109J for the treatment of dairy calves with experimentally induced infectious bovine keratoconjunctivitis.
[So] Source:Am J Vet Res;77(9):1017-28, 2016 Sep.
[Is] ISSN:1943-5681
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE To determine the efficacy of Bdellovibrio bacteriovorus 109J for the treatment of calves with experimentally induced infectious bovine keratoconjunctivitis (IBK). ANIMALS 12 healthy dairy calves. PROCEDURES For each calf, a grid keratotomy was performed on both eyes immediately before inoculation with Moraxella bovis hemolytic strain Epp63-300 (n = 11 calves) or nonhemolytic strain 12040577 (1 calf). For each calf inoculated with M bovis Epp63-300, the eyes were randomly assigned to receive an artificial tear solution with (treatment group) or without (control group) lyophilized B bacteriovorus 109J. Six doses of the assigned treatment (0.2 mL/eye, topically, q 48 h) were administered to each eye. On nontreatment days, eyes were assessed and corneal swab specimens and tear samples were collected for bacterial culture. Calves were euthanized 12 days after M bovis inoculation. The eyes were harvested for gross and histologic evaluation and bacterial culture. RESULTS The calf inoculated with M bovis 12040577 did not develop corneal ulcers. Of the 22 eyes inoculated with M bovis Epp63-300, 18 developed corneal ulcers consistent with IBK within 48 hours after inoculation; 4 of those eyes developed secondary corneal ulcers that were not consistent with IBK. Corneal ulcer size and severity and the time required for ulcer healing did not differ between the treatment and control groups. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that B bacteriovorus 109J was not effective for the treatment of IBK; however, the experimental model used produced lesions that did not completely mimic naturally occurring IBK.
[Mh] Termos MeSH primário: Bdellovibrio bacteriovorus
Doenças dos Bovinos/terapia
Conjuntivite Bacteriana/veterinária
Ceratoconjuntivite/veterinária
Infecções por Moraxellaceae/veterinária
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/microbiologia
Conjuntivite Bacteriana/microbiologia
Conjuntivite Bacteriana/terapia
Córnea
Ceratoconjuntivite/terapia
Ceratoconjuntivite Infecciosa/microbiologia
Masculino
Moraxella (Moraxella) bovis
Infecções por Moraxellaceae/microbiologia
Infecções por Moraxellaceae/terapia
Vacinação/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170123
[Lr] Data última revisão:
170123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160901
[St] Status:MEDLINE
[do] DOI:10.2460/ajvr.77.9.1017


  8 / 11 MEDLINE  
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[PMID]:27579919
[Au] Autor:Gupta S; Tang C; Tran M; Kadouri DE
[Ad] Endereço:Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, United States of America.
[Ti] Título:Effect of Predatory Bacteria on Human Cell Lines.
[So] Source:PLoS One;11(8):e0161242, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Predatory bacteria are Gram-negative bacteria that prey on other Gram-negative bacteria and have been considered as potential therapeutic agents against multi-drug resistant pathogens. In vivo animal models have demonstrated that predatory bacteria are non-toxic and non-immunogenic in rodents. In order to consider the use of predatory bacteria as live antibiotics, it is important to investigate their effect on human cells. The aim of this study was to determine the effect of Bdellovibrio bacteriovorus strains 109J and HD100, and Micavibrio aeruginosavorus strain ARL-13 on cell viability and inflammatory responses of five human cell lines, representative of clinically relevant tissues. We found that the predators were not cytotoxic to any of the human cell lines tested. Microscopic imaging showed no signs of cell detachment, as compared to predator-free cells. In comparison to an E. coli control, exposure to higher concentrations of the predators did not trigger a significant elevation of pro-inflammatory cytokines in four of the five human cell lines tested. Our work underlines the non-pathogenic attributes of predatory bacteria on human cells and highlights their potential use as live antibiotics against human pathogens.
[Mh] Termos MeSH primário: Bdellovibrio bacteriovorus/metabolismo
Infecções por Escherichia coli/metabolismo
Escherichia coli/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular
Seres Humanos
Inflamação/metabolismo
Inflamação/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160901
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0161242


  9 / 11 MEDLINE  
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[PMID]:27328268
[Au] Autor:Feng S; Tan CH; Cohen Y; Rice SA
[Ad] Endereço:The Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore.
[Ti] Título:Isolation of Bdellovibrio bacteriovorus from a tropical wastewater treatment plant and predation of mixed species biofilms assembled by the native community members.
[So] Source:Environ Microbiol;18(11):3923-3931, 2016 Nov.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:It is reported here that a predatory bacterium belonging to the Genus Bdellovibrio, was isolated from activated sludge at the Ulu Pandan Water Reclamation Plant, Singapore. 16S rDNA gene sequencing analysis revealed that this isolate was 99% identical to 'Bdellovibrio bacteriovorus strain Tiberius' and hence is designated as 'Bdellovibrio bacteriovorus UP'. Using a novel approach based on fluorescence in situ hybridization (FISH), a prey cell density-dependent growth pattern of B. bacteriovorus UP was established. B. bacteriovorus UP preyed upon a broad range of bacterial species (60 species) isolated from the activated sludge. Except for Ochrobactrum anthropi, all Gram-negative species were sensitive to predation by B. bacteriovorus UP irrespective of the mode of growth (planktonic or biofilm). Similarly, the predation-sensitive species were not protected by the predation-resistant species, O. anthropi, as determined in multiple dual-species planktonic and biofilm consortia. Given the broad prey spectrum, B. bacteriovorus UP may impact functional community members, which are largely members of the Proteobacteria. Thus, these results provide an important insight to the role of predatory bacteria in shaping of community structure and function in both natural and engineered ecosystems.
[Mh] Termos MeSH primário: Bdellovibrio bacteriovorus/isolamento & purificação
Bdellovibrio bacteriovorus/fisiologia
Biofilmes
Águas Residuais/microbiologia
[Mh] Termos MeSH secundário: Bdellovibrio bacteriovorus/genética
Biodegradação Ambiental
Biodiversidade
Hibridização in Situ Fluorescente
Purificação da Água/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160622
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13384


  10 / 11 MEDLINE  
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[PMID]:27106259
[Au] Autor:Spain EM; Núñez ME; Kim HJ; Taylor RJ; Thomas N; Wengen MB; Dalleska NF; Bromley JP; Schermerhorn KH; Ferguson MA
[Ad] Endereço:Department of Chemistry, Occidental College, 1600 Campus Rd, Los Angeles, CA 90041, USA. Electronic address: emspain@oxy.edu.
[Ti] Título:Identification and differential production of ubiquinone-8 in the bacterial predator Bdellovibrio bacteriovorus.
[So] Source:Res Microbiol;167(5):413-23, 2016 Jun.
[Is] ISSN:1769-7123
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Bdellovibrio bacteriovorus 109J, a predatory bacterium with potential as a bacterial control agent, can exist in several lifestyles that differ both in predatory capacity and color. We determined that levels of ubiquinone-8 contribute to the distinctive but variable yellow color of different types of Bdellovibrio cells. Steady-state ubiquinone-8 concentrations did not differ markedly between conventional predatory and host-independent B. bacteriovorus despite upregulation of a suite of ubiquinone-8 synthesis genes in host-independent cells. In contrast, in spatially organized B. bacteriovorus films, the yellow inner regions contain significantly higher ubiquinone-8 concentrations than the off-white outer regions. Correspondingly, RT-PCR analysis reveals that the inner region, previously shown to consist primarily of active predators, clearly expresses two ubiquinone biosynthesis genes, while the outer region, composed mainly of quiescent or stalled bdelloplasts, expresses those genes weakly or not at all. Moreover, B. bacteriovorus cells in the inner region of week-old interfacial films, which are phenotypically attack-phase, have much higher UQ8 levels than regular attack-phase bdellovibrios, most likely because their "trapped" state prevents a high expenditure of energy to power flagellar motion.
[Mh] Termos MeSH primário: Bdellovibrio bacteriovorus/metabolismo
Ubiquinona/metabolismo
[Mh] Termos MeSH secundário: Bdellovibrio bacteriovorus/genética
Bdellovibrio bacteriovorus/crescimento & desenvolvimento
Vias Biossintéticas
Perfilação da Expressão Gênica
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160424
[St] Status:MEDLINE



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