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Pesquisa : B03.440.400.425.200 [Categoria DeCS]
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[PMID]:28088676
[Au] Autor:Komaniecka I; Choma A; Zamlynska K; Sroka-Bartnicka A; Sowinski P
[Ad] Endereço:Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland. Electronic address: iwona.komaniecka@poczta.umcs.lublin.pl.
[Ti] Título:Structure of O-specific polysaccharide of Oligotropha carboxidovorans OM5 - a wastewater bacterium.
[So] Source:Carbohydr Res;439:30-34, 2017 Feb 01.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Oligotropha carboxidovorans strain OM5 (previously known as Pseudomonas carboxydovorans OM5) is a rod-shaped Gram-negative bacterium isolated from wastewater. This bacterium is able to live in aerobic and, facultatively, in autotrophic conditions. For autotrophic growth, the bacteria can utilize carbon monoxide or hydrogen as a source of energy. The O-specific polysaccharide isolated from O. carboxidovorans OM5 lipopolysaccharide was structurally characterized using chemical analyses, 1D and 2D NMR spectroscopy, and MALDI-TOF mass spectrometry techniques. The polysaccharide was found to be a homopolymer built up of 3-O-methyl-α-d-mannose residues linked by (1 â†’ 2)-glycosidic bonds. The degree of polymerization of high-molecular-weight polysaccharide was estimated at approximately 35-40 units. The structure of the homopolymer is depicted below: [Formula: see text].
[Mh] Termos MeSH primário: Bradyrhizobiaceae/química
Lipopolissacarídeos/química
Manose/análogos & derivados
Microbiologia da Água
[Mh] Termos MeSH secundário: Aerobiose
Processos Autotróficos
Bradyrhizobiaceae/metabolismo
Sequência de Carboidratos
Lipopolissacarídeos/isolamento & purificação
Espectroscopia de Ressonância Magnética
Peso Molecular
Polimerização
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Águas Residuais/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipopolysaccharides); 0 (Waste Water); PHA4727WTP (Mannose)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170116
[St] Status:MEDLINE


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[PMID]:27999092
[Au] Autor:Breglia R; Bruschi M; Cosentino U; De Gioia L; Greco C; Miyake T; Moro G
[Ad] Endereço:Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126Milan, Italy.
[Ti] Título:A theoretical study on the reactivity of the Mo/Cu-containing carbon monoxide dehydrogenase with dihydrogen.
[So] Source:Protein Eng Des Sel;30(3):167-172, 2017 Mar 01.
[Is] ISSN:1741-0134
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Mo/Cu-dependent CO dehydrogenase from Oligotropha carboxidovorans is an enzyme that is able to catalyze CO oxidation to CO2; moreover, it can also oxidize H2, thus eliciting a characteristic EPR signal. Interestingly, the Ag-substituted enzyme form proved unable to catalyze H2 oxidation. In the present contribution, we characterized the reactivity of the enzyme with H2 by quantum-chemical calculations. It was found that dihydrogen binding to the wild-type enzyme requires significant structural rearrangements of the active site Theoretical EPR spectra for plausible H2-bound models of the partially reduced, paramagnetic active site are also presented and compared with the experimental counterpart. Finally, density functional theory modeling shows that Ag substitution impairs H2 binding at the active site.
[Mh] Termos MeSH primário: Aldeído Oxirredutases/química
Proteínas de Bactérias/química
Bradyrhizobiaceae/enzimologia
Hidrogênio/química
Modelos Químicos
Complexos Multienzimáticos/química
Prata/química
[Mh] Termos MeSH secundário: Cobre/química
Espectroscopia de Ressonância de Spin Eletrônica
Molibdênio/química
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Multienzyme Complexes); 3M4G523W1G (Silver); 789U1901C5 (Copper); 7YNJ3PO35Z (Hydrogen); 81AH48963U (Molybdenum); EC 1.2.- (Aldehyde Oxidoreductases); EC 1.2.99.2 (carbon monoxide dehydrogenase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1093/protein/gzw071


  3 / 106 MEDLINE  
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[PMID]:27902203
[Au] Autor:Dahal RH; Kim J
[Ad] Endereço:Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
[Ti] Título:Microvirga soli sp. nov., an alphaproteobacterium isolated from soil.
[So] Source:Int J Syst Evol Microbiol;67(1):127-132, 2017 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An aerobic, Gram-stain-negative, catalase-positive and oxidase-negative, non-motile, non-spore-forming, rod-shaped, pink-pigmented bacterium designated strain R491T was isolated from soil. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain R491T formed a lineage within the family Methylobacteriaceae of the phylum Proteobacteria that was distinct from various species of the genus Microvirga, including Microvirgaaerilata 5420S-16T (97.83 % 16S rRNA gene sequence similarity), Microvirga zambiensis WSM3693T (97.76 %), Microvirga flocculans ATCC BAA-817T (97.41 %), and Microvirga lupini Lut6T, Microvirga subterranea DSM 14364T, Microvirga vignae BR3299T, and Microvirga guangxiensis 25BT (96.99 %). The major polar lipids of strain R491T were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 71.2 %), C16 : 0 (12.0 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 4.7 %), and C18 : 0 (4.2 %). The DNA G+C content of strain R491T was 61.8 mol%. DNA-DNA hybridization values between strain R491T and other members of the genus Microvirga ranged from 27 to 57 %. On the basis of phenotypic, genotypic and phylogenetic analyses, strain R491T represents a novel species of the genus Microvirga, for which the name Microvirga soli sp. nov. is proposed. The type strain is R491T (=KEMB 9005-408T=KACC 18969T=NBRC 112417T).
[Mh] Termos MeSH primário: Methylobacteriaceae/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Bradyrhizobiaceae/genética
DNA Bacteriano/genética
Ácidos Graxos/análise
Methylobacteriaceae/genética
Methylobacteriaceae/isolamento & purificação
Hibridização de Ácido Nucleico
Pigmentação
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001582


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[PMID]:27418402
[Au] Autor:Walczak AB; Kafantaris FA; Druschel GK; Yee N; Young LY
[Ad] Endereço:Department of Environmental Sciences, Rutgers University, New Brunswick, NJ, USA.
[Ti] Título:Transformation of galena to pyromorphite produces bioavailable sulfur for neutrophilic chemoautotrophy.
[So] Source:Geobiology;14(6):599-606, 2016 11.
[Is] ISSN:1472-4669
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aqueous concentration of lead [Pb(II)] in geochemical environments is controlled by the solubility of Pb-bearing minerals and their weathering products. In contaminated soils, a common method for in situ stabilization of Pb(II) is the addition of phosphate to convert more redox sensitive sulfide minerals into sparingly soluble pyromorphite [Pb (PO ) X]. In this study, we conducted experimental studies to investigate the fate of reduced sulfur during the conversion of galena [PbS] to chloropyromorphite [Pb (PO ) Cl]. Powder X-ray diffraction analysis indicated that the reaction of phosphate with galena under oxic conditions resulted in the oxidation of sulfide and formation of elemental sulfur [S ]. Under oxic abiotic conditions, the S was retained in the solid phase, and negligible concentrations of sulfur as sulfide and thiosulfate were detected in the aqueous phase and only a small amount of sulfate. When PbS reacted in the presence of the chemoautotrophic organism Bosea sp. WAO, the S in the secondary mineral was oxidized to sulfate. Strain WAO produced significantly more sulfate from the secondary S than from the primary galena. Microscopic analysis of mineral-microbe aggregates on mineral-embedded slide cultures showed that the organism was colocalized and increased in biomass over time on the secondary mineral surface supporting a microbial role. The results of this study indicate that stimulation of sulfur-oxidizing activity may be a direct consequence of phosphate amendments to Pb(II)-contaminated soils.
[Mh] Termos MeSH primário: Bradyrhizobiaceae/crescimento & desenvolvimento
Bradyrhizobiaceae/metabolismo
Crescimento Quimioautotrófico
Chumbo/química
Minerais/química
Fosfatos/química
Sulfetos/química
Enxofre/metabolismo
[Mh] Termos MeSH secundário: Disponibilidade Biológica
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Minerals); 0 (Phosphates); 0 (Sulfides); 12190-77-1 (pyromorphite); 2425D15SYM (lead sulfide); 2P299V784P (Lead); 70FD1KFU70 (Sulfur)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170419
[Lr] Data última revisão:
170419
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160716
[St] Status:MEDLINE
[do] DOI:10.1111/gbi.12199


  5 / 106 MEDLINE  
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[PMID]:26555469
[Au] Autor:Bol'shakov MA; Ashikhmin AA; Makhneva ZK; Moskalenko AA
[Ad] Endereço:Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. andrey-moskalenko@rambler.ru.
[Ti] Título:Peripheral Light-Harvesting LH2 Complex Can Be Assembled in Cells of Nonsulfur Purple Bacterium Rhodoblastus acidophilus without Carotenoids.
[So] Source:Biochemistry (Mosc);80(9):1169-77, 2015 Sep.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effect of carotenoids on the assembly of LH2 complex in cells of the purple nonsulfur bacterium Rhodoblastus acidophilus was investigated. For this purpose, the bacterial culture was cultivated with an inhibitor of carotenoid biosynthesis - 71 µM diphenylamine (DPA). The inhibitor decreased the level of biosynthesis of the colored carotenoids in membranes by ~58%. It was found that a large amount of phytoene was accumulated in them. This carotenoid precursor was bound nonspecifically to LH2 complex and did not stabilize its structure. Thermostability testing of the isolated LH2 complex together with analysis of carotenoid composition revealed that the population of this complex was heterogeneous with respect to carotenoid composition. One fraction of the LH2 complex with carotenoid content around 90% remains stable and was not destroyed under heating for 15 min at 50°C. The other fraction of LH2 complex containing on average less than one molecule of carotenoid per complex was destroyed under heating, forming a zone of free pigments (and polypeptides). The data suggest that a certain part of the LH2 complexes is assembled without carotenoids in cells of the nonsulfur bacterium Rbl. acidophilus grown with DPA. These data contradict the fact that the LH2 complex from nonsulfur bacteria cannot be assembled without carotenoids, but on the other hand, they are in good agreement with the results demonstrated in our earlier studies of the sulfur bacteria Allochromatium minutissimum and Ectothiorhodospira haloalkaliphila. Carotenoidless LH2 complex was obtained from these bacteria with the use of DPA (Moskalenko, A. A., and Makhneva, Z. K. (2012) J. Photochem. Photobiol., 108, 1-7; Ashikhmin, A., et al. (2014) Photosynth. Res., 119, 291-303).
[Mh] Termos MeSH primário: Alphaproteobacteria/fisiologia
Proteínas de Bactérias/fisiologia
Bradyrhizobiaceae/fisiologia
Carotenoides/fisiologia
Complexos de Proteínas Captadores de Luz/fisiologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/efeitos dos fármacos
Bradyrhizobiaceae/química
Bradyrhizobiaceae/citologia
Carotenoides/antagonistas & inibidores
Difenilamina/farmacologia
Complexos de Proteínas Captadores de Luz/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (B800-850 light-harvesting complex, bacteria); 0 (Bacterial Proteins); 0 (Light-Harvesting Protein Complexes); 36-88-4 (Carotenoids); 9N3CBB0BIQ (Diphenylamine)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151111
[Lr] Data última revisão:
151111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151112
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297915090072


  6 / 106 MEDLINE  
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[PMID]:26067561
[Au] Autor:Chao Y; Mao Y; Wang Z; Zhang T
[Ad] Endereço:1] Environmental Biotechnology Laboratory, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China [2] School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou, China.
[Ti] Título:Diversity and functions of bacterial community in drinking water biofilms revealed by high-throughput sequencing.
[So] Source:Sci Rep;5:10044, 2015 Jun 12.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The development of biofilms in drinking water (DW) systems may cause various problems to water quality. To investigate the community structure of biofilms on different pipe materials and the global/specific metabolic functions of DW biofilms, PCR-based 454 pyrosequencing data for 16S rRNA genes and Illumina metagenomic data were generated and analysed. Considerable differences in bacterial diversity and taxonomic structure were identified between biofilms formed on stainless steel and biofilms formed on plastics, indicating that the metallic materials facilitate the formation of higher diversity biofilms. Moreover, variations in several dominant genera were observed during biofilm formation. Based on PCA analysis, the global functions in the DW biofilms were similar to other DW metagenomes. Beyond the global functions, the occurrences and abundances of specific protective genes involved in the glutathione metabolism, the SoxRS system, the OxyR system, RpoS regulated genes, and the production/degradation of extracellular polymeric substances were also evaluated. A near-complete and low-contamination draft genome was constructed from the metagenome of the DW biofilm, based on the coverage and tetranucleotide frequencies, and identified as a Bradyrhizobiaceae-like bacterium according to a phylogenetic analysis. Our findings provide new insight into DW biofilms, especially in terms of their metabolic functions.
[Mh] Termos MeSH primário: Biofilmes
Bradyrhizobiaceae
Água Potável/microbiologia
Sequenciamento de Nucleotídeos em Larga Escala
Consórcios Microbianos/fisiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Bradyrhizobiaceae/classificação
Bradyrhizobiaceae/fisiologia
Filogenia
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drinking Water); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150619
[Lr] Data última revisão:
150619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150613
[St] Status:MEDLINE
[do] DOI:10.1038/srep10044


  7 / 106 MEDLINE  
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[PMID]:26013968
[Au] Autor:Safronova VI; Kuznetsova IG; Sazanova AL; Kimeklis AK; Belimov AA; Andronov EE; Pinaev AG; Pukhaev AR; Popov KP; Akopian JA; Willems A; Tikhonovich IA
[Ad] Endereço:All-Russia Research Institute for Agricultural Microbiology (ARRIAM), St. Petersburg, Russian Federation, v.safronova@rambler.ru.
[Ti] Título:Extra-slow-growing Tardiphaga strains isolated from nodules of Vavilovia formosa (Stev.) Fed.
[So] Source:Arch Microbiol;197(7):889-98, 2015 Sep.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Eleven extra-slow-growing strains were isolated from nodules of the relict legume Vavilovia formosa growing in North Ossetia (Caucasus) and Armenia. All isolates formed a single rrs cluster together with the type strain Tardiphaga robiniae LMG 26467(T), while the sequencing of the 16S-23S rDNA intergenic region (ITS) and housekeeping genes glnII, atpD, dnaK, gyrB, recA and rpoB divided them into three groups. North Ossetian isolates (in contrast to the Armenian ones) were clustered separately from the type strain LMG 26467(T). However, all isolates were classified as T. robiniae because the DNA-DNA relatedness between them and the type strain LMG 26467(T) was 69.6% minimum. Two symbiosis-related genes (nodM and nodT) were amplified in all isolated Tardiphaga strains. It was shown that the nodM gene phylogeny is similar to that of ITS and housekeeping genes. The presence of the other symbiosis-related genes in described Tardiphaga strains, which is recently described genus of rhizobia, as well as their ability to form nodules on any plants are under investigation.
[Mh] Termos MeSH primário: Bradyrhizobiaceae/classificação
Bradyrhizobiaceae/fisiologia
Fabaceae/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Bradyrhizobiaceae/genética
Bradyrhizobiaceae/crescimento & desenvolvimento
Bradyrhizobiaceae/isolamento & purificação
DNA Bacteriano/genética
Filogenia
RNA Ribossômico 16S/genética
Simbiose/genética
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150814
[Lr] Data última revisão:
150814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150528
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-015-1122-3


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[PMID]:25941999
[Au] Autor:Zhang H; Liu L; Chang Q; Wang H; Yang K
[Ad] Endereço:School of Civil Engineering, Wuhan University, East Lake South Road 8, Wuchang District, Wuhan 430072, People's Republic of China.
[Ti] Título:Biosorption of Cr(VI) ions from aqueous solutions by a newly isolated Bosea sp. strain Zer-1 from soil samples of a refuse processing plant.
[So] Source:Can J Microbiol;61(6):399-408, 2015 Jun.
[Is] ISSN:1480-3275
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:The adsorption behavior of Cr(VI) ions from aqueous solution by a chromium-tolerant strain was studied through batch experiments. An isolate designated Zer-1 was identified as a species of Bosea on the basis of 16S rRNA results. It showed a maximum resistance to 550 mg·L(-1) Cr(VI). The effects of 3 important operating parameters, initial solution pH, initial Cr(VI) concentration, and biomass dose, were investigated by central composite design. On the basis of response surface methodology results, maximal removal efficiency of Cr(VI) was achieved under the following conditions: pH, 2.0; initial concentration of metal ions, 55 mg·L(-1); and biomass dose, 2.0 g·L(-1). Under the optimal conditions, the maximum removal efficiency of Cr(VI) ions was found to be nearly 98%. The experimental data exhibited a better fit with the Langmuir model than the Freundlich model. The biosorption mechanisms were investigated with pseudo-first-order, pseudo-second-order, and intraparticle diffusion kinetics models. These results revealed that biosorption of Cr(VI) onto bacterial biomass could be an alternative method for the removal of metal ions from aqueous solution.
[Mh] Termos MeSH primário: Bradyrhizobiaceae/metabolismo
Cromo/metabolismo
Recuperação e Remediação Ambiental/instrumentação
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Adsorção
Biodegradação Ambiental
Bradyrhizobiaceae/classificação
Bradyrhizobiaceae/genética
Bradyrhizobiaceae/isolamento & purificação
Cromo/química
Concentração de Íons de Hidrogênio
Íons/metabolismo
Cinética
Dados de Sequência Molecular
Filogenia
Solo/química
Microbiologia do Solo
Poluentes Químicos da Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ions); 0 (Soil); 0 (Water Pollutants, Chemical); 0R0008Q3JB (Chromium); 18540-29-9 (chromium hexavalent ion)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150506
[St] Status:MEDLINE
[do] DOI:10.1139/cjm-2014-0719


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[PMID]:25874801
[Au] Autor:Pickering BS; Tyler S; Smith G; Burton L; Li M; Dallaire A; Weingartl H
[Ad] Endereço:National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada; Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
[Ti] Título:Identification of a novel Afipia species isolated from an Indian flying fox.
[So] Source:PLoS One;10(4):e0121274, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An old world fruit bat Pteropus giganteus, held in captivity and suffering from necrosis of its wing digits, failed to respond to antibiotic therapy and succumbed to the infection. Samples submitted to the National Centre for Foreign Animal Disease were tested for viral infection. Vero E6 cells exhibited minor but unique cytopathic effects on second blind passage, and full CPE by passage four. Utilizing an unbiased random amplification technique from cell culture supernatant, we identified a bacterium belonging to the Bradyrhizobiaceae. Purification of cell culture supernatant on TY media revealed a slow growing bacterial isolate. In this study using electron microscopy, 16S rRNA gene analysis and whole genome sequencing, we identify a novel bacterial species associated with the site of infection belonging to the genus Afipia. This genus of bacteria is very diverse, with only a limited number of species characterized. Afipia felis, previously described as the etiological agent to cause cat scratch disease, and Afipia septicemium, most recently shown to cause disease in humans, highlight the potential for members of this genus to form a branch of opportunistic pathogens within the Bradyrhizobiaceae. Increased utilization of next generation sequencing and genomics will aid in classifying additional members of this intriguing bacterial genera.
[Mh] Termos MeSH primário: Bradyrhizobiaceae/isolamento & purificação
Quirópteros/microbiologia
Necrose/microbiologia
Asas de Animais/microbiologia
[Mh] Termos MeSH secundário: Afipia/patogenicidade
Animais
Bradyrhizobiaceae/genética
Bradyrhizobiaceae/patogenicidade
Doença da Arranhadura de Gato/genética
Doença da Arranhadura de Gato/microbiologia
Gatos
Genoma Bacteriano
Seres Humanos
Necrose/fisiopatologia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Asas de Animais/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150422
[Lr] Data última revisão:
150422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150416
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0121274


  10 / 106 MEDLINE  
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[PMID]:25753751
[Au] Autor:Quandt CA; Kohler A; Hesse CN; Sharpton TJ; Martin F; Spatafora JW
[Ad] Endereço:Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331, USA.
[Ti] Título:Metagenome sequence of Elaphomyces granulatus from sporocarp tissue reveals Ascomycota ectomycorrhizal fingerprints of genome expansion and a Proteobacteria-rich microbiome.
[So] Source:Environ Microbiol;17(8):2952-68, 2015 Aug.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many obligate symbiotic fungi are difficult to maintain in culture, and there is a growing need for alternative approaches to obtaining tissue and subsequent genomic assemblies from such species. In this study, the genome of Elaphomyces granulatus was sequenced from sporocarp tissue. The genome assembly remains on many contigs, but gene space is estimated to be mostly complete. Phylogenetic analyses revealed that the Elaphomyces lineage is most closely related to Talaromyces and Trichocomaceae s.s. The genome of E. granulatus is reduced in carbohydrate-active enzymes, despite a large expansion in genome size, both of which are consistent with what is seen in Tuber melanosporum, the other sequenced ectomycorrhizal ascomycete. A large number of transposable elements are predicted in the E. granulatus genome, especially Gypsy-like long terminal repeats, and there has also been an expansion in helicases. The metagenome is a complex community dominated by bacteria in Bradyrhizobiaceae, and there is evidence to suggest that the community may be reduced in functional capacity as estimated by KEGG pathways. Through the sequencing of sporocarp tissue, this study has provided insights into Elaphomyces phylogenetics, genomics, metagenomics and the evolution of the ectomycorrhizal association.
[Mh] Termos MeSH primário: Bradyrhizobiaceae/genética
DNA Fúngico/genética
Eurotiales/genética
Carpóforos/genética
Genoma Fúngico/genética
Metagenoma
[Mh] Termos MeSH secundário: Sequência de Bases
Bradyrhizobiaceae/classificação
Elementos de DNA Transponíveis/genética
Eurotiales/classificação
Metagenômica
Microbiota/genética
Micorrizas/genética
Filogenia
Análise de Sequência de DNA
Talaromyces/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Fungal)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150902
[Lr] Data última revisão:
150902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150311
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.12840



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