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  1 / 3213 MEDLINE  
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[PMID]:27771184
[Au] Autor:van Straten M; Bardenstein S; Keningswald G; Banai M
[Ad] Endereço:"Hachaklait", Mutual Society for Veterinary Services, P.O.B. 3039, Caesarea Industrial Park, 38900, Israel. Electronic address: vanstraten@hachaklait.co.il.
[Ti] Título:Brucella abortus S19 vaccine protects dairy cattle against natural infection with Brucella melitensis.
[So] Source:Vaccine;34(48):5837-5839, 2016 11 21.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Brucellosis is a zoonotic disease that can cause severe illness in humans and considerable economic loss in the livestock industry. Although small ruminants are the preferential host for Brucella melitensis, this pathogen has emerged as a cause for Brucella outbreaks in cattle. S19 vaccination is implemented in many countries where B. abortus is endemic but its effectiveness against B. melitensis has not been validated. Here we show that vaccine effectiveness in preventing disease transmission between vaccinated and unvaccinated cohorts, as determined by seroconversion, was 87.2% (95% CI 69.5-94.6%). Furthermore, vaccination was associated with a reduced risk for abortion. Together, our data emphasize the role S19 vaccination could play in preventing B. melitensis outbreaks in areas where this pathogen is prevalent in small ruminant populations.
[Mh] Termos MeSH primário: Aborto Animal/prevenção & controle
Vacina contra Brucelose/administração & dosagem
Brucella melitensis/imunologia
Brucelose Bovina/prevenção & controle
[Mh] Termos MeSH secundário: Aborto Animal/microbiologia
Animais
Anticorpos Antibacterianos
Vacina contra Brucelose/imunologia
Brucella abortus/imunologia
Brucella melitensis/patogenicidade
Brucelose Bovina/transmissão
Bovinos
Proteção Cruzada
Feminino
Gravidez
Complicações Infecciosas na Gravidez/prevenção & controle
Complicações Infecciosas na Gravidez/veterinária
Soroconversão
Vacinação/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Brucella Vaccine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  2 / 3213 MEDLINE  
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[PMID]:29041788
[Au] Autor:Chisi SL; Schmidt T; Akol GW; Van Heerden H
[Ad] Endereço:Department of Agriculture and Rural Development KwaZulu Natal Province, Allerton Provincial Veterinary Laboratory. songechisi@gmail.com.
[Ti] Título:Use of Brucella abortus species specific polymerase chain reaction assay for the diagnosis of bovine brucellosis.
[So] Source:J S Afr Vet Assoc;88(0):e1-e3, 2017 Sep 27.
[Is] ISSN:2224-9435
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Serology is primarily used in the diagnosis of bovine brucellosis. Bacterial culture and isolation is the gold standard in diagnosing brucellosis but, like serology, it does not offer complete (100%) diagnostic sensitivity and specificity. Polymerase chain reaction (PCR) has been suggested to offer better specificity and sensitivity. In this study, we evaluated the performance of Brucella abortus species specific (BaSS) PCR directly from different samples in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture. The BaSS PCR had a low diagnostic sensitivity (DSe) of 70%, but was able to identify vaccine strains using abomasal fluid from aborted foetuses and detect Brucella DNA from decomposing samples. The best sample for the BaSS PCR was abomasal fluid.
[Mh] Termos MeSH primário: Brucella abortus/isolamento & purificação
Brucelose Bovina/microbiologia
[Mh] Termos MeSH secundário: Abomaso/microbiologia
Animais
Brucella abortus/genética
Bovinos
Feminino
Feto/microbiologia
Leite/microbiologia
Reação em Cadeia da Polimerase/veterinária
Gravidez
Sensibilidade e Especificidade
Vacinação/veterinária
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171019
[St] Status:MEDLINE
[do] DOI:10.4102/jsava.v88i0.1433


  3 / 3213 MEDLINE  
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[PMID]:28808159
[Au] Autor:Machelart A; Khadrawi A; Demars A; Willemart K; De Trez C; Letesson JJ; Muraille E
[Ad] Endereço:Unité de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, Université de Namur, Namur, Belgium.
[Ti] Título:Chronic Brucella Infection Induces Selective and Persistent Interferon Gamma-Dependent Alterations of Marginal Zone Macrophages in the Spleen.
[So] Source:Infect Immun;85(11), 2017 Nov.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The spleen is known as an important filter for blood-borne pathogens that are trapped by specialized macrophages in the marginal zone (MZ): the CD209 MZ macrophages (MZMs) and the CD169 marginal metallophilic macrophages (MMMs). Acute systemic infection strongly impacts MZ populations and the location of T and B lymphocytes. This phenomenon has been linked to reduced chemokine secretion by stromal cells. spp. are the causative agent of brucellosis, a widespread zoonotic disease. Here, we used infection as a model to investigate the impact of chronic stealth infection on splenic MZ macrophage populations. During the late phase of infection, we observed a loss of both MZMs and MMMs, with a durable disappearance of MZMs, leading to a reduction of the ability of the spleen to take up soluble antigens, beads, and unrelated bacteria. This effect appears to be selective as every other lymphoid and myeloid population analyzed increased during infection, which was also observed following and infection. Comparison of wild-type and deficient mice suggested that MZ macrophage population loss is dependent on interferon gamma (IFN-γ) receptor but independent of T cells or tumor necrosis factor alpha receptor 1 (TNF-αR1) signaling pathways and is not correlated to an alteration of CCL19, CCL21, and CXCL13 chemokine mRNA expression. Our results suggest that MZ macrophage populations are particularly sensitive to persistent low-level IFN-γ-mediated inflammation and that infection could reduce the ability of the spleen to perform certain MZM- and MMM-dependent tasks, such as antigen delivery to lymphocytes and control of systemic infection.
[Mh] Termos MeSH primário: Brucelose/imunologia
Interações Hospedeiro-Patógeno
Interferon gama/imunologia
Macrófagos/imunologia
Receptores de Interferon/imunologia
Baço/imunologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Linfócitos B/imunologia
Linfócitos B/microbiologia
Brucella abortus/efeitos dos fármacos
Brucella abortus/imunologia
Brucella abortus/patogenicidade
Brucella melitensis/efeitos dos fármacos
Brucella melitensis/imunologia
Brucella melitensis/patogenicidade
Brucella suis/efeitos dos fármacos
Brucella suis/imunologia
Brucella suis/patogenicidade
Brucelose/tratamento farmacológico
Brucelose/genética
Brucelose/microbiologia
Quimiocina CCL19/genética
Quimiocina CCL19/imunologia
Quimiocina CCL21/genética
Quimiocina CCL21/imunologia
Quimiocina CXCL13/genética
Quimiocina CXCL13/imunologia
Doença Crônica
Regulação da Expressão Gênica
Interferon gama/genética
Macrófagos/microbiologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
RNA Mensageiro/genética
RNA Mensageiro/imunologia
Receptores de Interferon/deficiência
Receptores de Interferon/genética
Receptores Tipo I de Fatores de Necrose Tumoral/deficiência
Receptores Tipo I de Fatores de Necrose Tumoral/genética
Receptores Tipo I de Fatores de Necrose Tumoral/imunologia
Rifampina/farmacologia
Transdução de Sinais
Baço/microbiologia
Estreptomicina/farmacologia
Linfócitos T/imunologia
Linfócitos T/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Ccl19 protein, mouse); 0 (Chemokine CCL19); 0 (Chemokine CCL21); 0 (Chemokine CXCL13); 0 (Cxcl13 protein, mouse); 0 (RNA, Messenger); 0 (Receptors, Interferon); 0 (Receptors, Tumor Necrosis Factor, Type I); 0 (interferon gamma receptor); 82115-62-6 (Interferon-gamma); VJT6J7R4TR (Rifampin); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE


  4 / 3213 MEDLINE  
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[PMID]:28797045
[Au] Autor:Hernández-Mora G; Ruiz-Villalobos N; Bonilla-Montoya R; Romero-Zúniga JJ; Jiménez-Arias J; González-Barrientos R; Barquero-Calvo E; Chacón-Díaz C; Rojas N; Chaves-Olarte E; Guzmán-Verri C; Moreno E
[Ad] Endereço:Servicio Nacional de Salud Animal (SENASA), Ministerio de Agricultura y Ganadería, Heredia, Costa Rica.
[Ti] Título:Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease.
[So] Source:PLoS One;12(8):e0182380, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Brucellosis, caused by Brucella abortus is a major disease of cattle and a zoonosis. In order to estimate the bovine brucellosis prevalence in Costa Rica (CR), a total 765 herds (13078 bovines) from six regions of CR were randomly sampled during 2012-2013. A non-random sample of 7907 herds (532199 bovines) of the six regions, arriving for diagnoses during 2014-2016 to the Costa Rican Animal Health Service was also studied. The prevalence estimated by Rose Bengal test (RBT) ranged from 10.5%-11.4%; alternatively, the prevalence estimated by testing the RBT positives in iELISA, ranged from 4.1%-6.0%, respectively. However, cattle in CR are not vaccinated with B. abortus S19 but with RB51 (vaccination coverage close to 11%), and under these conditions the RBT displays 99% specificity and 99% sensitivity. Therefore, the RBT herd depicted in the random analysis stands as a feasible assessment and then, the recommended value in case of planning an eradication program in CR. Studies of three decades reveled that bovine brucellosis prevalence has increased in CR. B. abortus was identified by biochemical and molecular studies as the etiological agent of bovine brucellosis. Multiple locus variable-number tandem repeat analysis-16 revealed four B. abortus clusters. Cluster one and three are intertwined with isolates from other countries, while clusters two and four have only representatives from CR. Cluster one is widely distributed in all regions of the country and may be the primary B. abortus source. The other clusters seem to be restricted to specific areas in CR. The implications of our findings, in relation to the control of the disease in CR, are critically discussed.
[Mh] Termos MeSH primário: Brucelose Bovina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Brucella abortus/genética
Brucella abortus/isolamento & purificação
Brucelose Bovina/microbiologia
Bovinos
Costa Rica/epidemiologia
Feminino
Tipagem Molecular
Prevalência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182380


  5 / 3213 MEDLINE  
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[PMID]:28767704
[Au] Autor:Milillo MA; Velásquez LN; Trotta A; Delpino MV; Marinho FV; Balboa L; Vermeulen M; Espindola SL; Rodriguez-Rodrigues N; Fernández GC; Oliveira SC; Giambartolomei GH; Barrionuevo P
[Ad] Endereço:Instituto de Medicina Experimental (CONICET-Academia Nacional de Medicina), Buenos Aires, Argentina.
[Ti] Título:B. abortus RNA is the component involved in the down-modulation of MHC-I expression on human monocytes via TLR8 and the EGFR pathway.
[So] Source:PLoS Pathog;13(8):e1006527, 2017 Aug.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite eliciting a potent CD8+ T cell response, Brucella abortus is able to persist and establish a chronic infection inside its host. We have previously reported that the infection of human monocytes/macrophages with B. abortus inhibits the IFN-γ-induced MHC-I cell surface expression down-modulating cytotoxic CD8+ T cell responses. MHC-I down-modulation depends on bacterial viability and results from the capacity of B. abortus to retain the MHC-I molecules within the Golgi apparatus. Furthermore, we recently demonstrated that epidermal growth factor receptor (EGFR) pathway is involved in this phenomenon and that this is an early event during infection. However, the components and mechanisms whereby B. abortus is able to down-modulate MHC-I remained to be elucidated. In this study we demonstrated that the down-modulation of MHC-I expression is not mediated by well-known Brucella virulence factors but instead by B. abortus RNA, a PAMP associated to viability (vita-PAMP). Surprisingly, completely degraded RNA was also able to inhibit MHC-I expression to the same extent as intact RNA. Accordingly, B. abortus RNA and its degradation products were able to mimic the MHC-I intracellular retention within the Golgi apparatus observed upon infection. We further demonstrated that TLR8, a single-stranded RNA and RNA degradation products sensor, was involved in MHC-I inhibition. On the other hand, neutralization of the EGFR reversed the MHC-I inhibition, suggesting a connection between the TLR8 and EGFR pathways. Finally, B. abortus RNA-treated macrophages display diminished capacity of antigen presentation to CD8+ T cells. Overall, our results indicate that the vita-PAMP RNA as well as its degradation products constitute novel virulence factors whereby B. abortus, by a TLR8-dependent mechanism and through the EGFR pathway, inhibits the IFN-γ-induced MHC-I surface expression on human monocytes/macrophages. Thus, bacteria can hide within infected cells and avoid the immunological surveillance of cytotoxic CD8+ T cells.
[Mh] Termos MeSH primário: Brucelose/imunologia
Evasão da Resposta Imune/imunologia
Monócitos/imunologia
RNA Bacteriano/imunologia
Receptor do Fator de Crescimento Epidérmico/imunologia
Receptor 8 Toll-Like/imunologia
[Mh] Termos MeSH secundário: Animais
Brucella abortus/imunologia
Apresentação Cruzada/imunologia
Regulação para Baixo
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Antígenos de Histocompatibilidade Classe I/biossíntese
Seres Humanos
Camundongos
Camundongos Endogâmicos C57BL
Microscopia Confocal
Monócitos/microbiologia
Transdução de Sinais/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histocompatibility Antigens Class I); 0 (RNA, Bacterial); 0 (TLR8 protein, human); 0 (Toll-Like Receptor 8); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006527


  6 / 3213 MEDLINE  
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[PMID]:28609437
[Au] Autor:Brennan A; Cross PC; Portacci K; Scurlock BM; Edwards WH
[Ad] Endereço:Wyoming Cooperative Fish and Wildlife Research Unit, Department of Zoology and Physiology, University of Wyoming, Laramie, Wyoming, United States of America.
[Ti] Título:Shifting brucellosis risk in livestock coincides with spreading seroprevalence in elk.
[So] Source:PLoS One;12(6):e0178780, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tracking and preventing the spillover of disease from wildlife to livestock can be difficult when rare outbreaks occur across large landscapes. In these cases, broad scale ecological studies could help identify risk factors and patterns of risk to inform management and reduce incidence of disease. Between 2002 and 2014, 21 livestock herds in the Greater Yellowstone Area (GYA) were affected by brucellosis, a bacterial disease caused by Brucella abortus, while no affected herds were detected between 1990 and 2001. Using a Bayesian analysis, we examined several ecological covariates that may be associated with affected livestock herds across the region. We showed that livestock risk has been increasing over time and expanding outward from the historical nexus of brucellosis in wild elk on Wyoming's feeding grounds where elk are supplementally fed during the winter. Although elk were the presumed source of cattle infections, occurrences of affected livestock herds were only weakly associated with the density of seropositive elk across the GYA. However, the shift in livestock risk did coincide with recent increases in brucellosis seroprevalence in unfed elk populations. As increasing brucellosis in unfed elk likely stemmed from high levels of the disease in fed elk, disease-related costs of feeding elk have probably been incurred across the entire GYA, rather than solely around the feeding grounds. Our results suggest that focused disease mitigation in areas where seroprevalence in unfed elk is high could reduce the spillover of brucellosis to livestock. We also highlight the need to better understand the epidemiology of spillover events with detailed histories of disease testing, calving, and movement of infected livestock. Finally, we recommend using case-control studies to investigate local factors important to livestock risk.
[Mh] Termos MeSH primário: Animais Selvagens/microbiologia
Brucella abortus/fisiologia
Brucelose/microbiologia
Cervos/microbiologia
Gado/microbiologia
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Bison
Brucelose/epidemiologia
Bovinos
Surtos de Doenças/veterinária
Geografia
Interações Hospedeiro-Patógeno
Idaho/epidemiologia
Incidência
Modelos Teóricos
Montana/epidemiologia
Fatores de Risco
Estudos Soroepidemiológicos
Wyoming/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178780


  7 / 3213 MEDLINE  
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[PMID]:28559292
[Au] Autor:Czyz DM; Willett JW; Crosson S
[Ad] Endereço:Howard Taylor Ricketts Laboratory, University of Chicago, Argonne National Laboratory, Lemont, Illinois, USA.
[Ti] Título:Brucella abortus Induces a Warburg Shift in Host Metabolism That Is Linked to Enhanced Intracellular Survival of the Pathogen.
[So] Source:J Bacteriol;199(15), 2017 Aug 01.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intracellular bacterial pathogens exploit host cell resources to replicate and survive inside the host. Targeting these host systems is one promising approach to developing novel antimicrobials to treat intracellular infections. We show that human macrophage-like cells infected with undergo a metabolic shift characterized by attenuated tricarboxylic acid cycle metabolism, reduced amino acid consumption, altered mitochondrial localization, and increased lactate production. This shift to an aerobic glycolytic state resembles the Warburg effect, a change in energy production that is well described in cancer cells and also occurs in activated inflammatory cells. efficiently uses lactic acid as its sole carbon and energy source and requires the ability to metabolize lactate for normal survival in human macrophage-like cells. We demonstrate that chemical inhibitors of host glycolysis and lactate production do not affect growth of in axenic culture but decrease its survival in the intracellular niche. Our data support a model in which infection shifts host metabolism to a Warburg-like state, and uses this change in metabolism to promote intracellular survival. Pharmacological perturbation of these features of host cell metabolism may be a useful strategy to inhibit infection by intracellular pathogens. spp. are intracellular bacterial pathogens that cause disease in a range of mammals, including livestock. Transmission from livestock to humans is common and can lead to chronic human disease. Human macrophage-like cells infected with undergo a Warburg-like metabolic shift to an aerobic glycolytic state where the host cells produce lactic acid and have reduced amino acid catabolism. We provide evidence that the pathogen can exploit this change in host metabolism to support growth and survival in the intracellular niche. Drugs that inhibit this shift in host cell metabolism inhibit intracellular replication and decrease the survival of in an infection model; these drugs may be broadly useful therapeutics for intracellular infections.
[Mh] Termos MeSH primário: Brucella abortus/fisiologia
Glicólise
Interações Hospedeiro-Patógeno
Viabilidade Microbiana
Monócitos/microbiologia
[Mh] Termos MeSH secundário: Anaerobiose
Brucella abortus/crescimento & desenvolvimento
Brucella abortus/metabolismo
Linhagem Celular
Metabolismo Energético
Seres Humanos
Ácido Láctico/metabolismo
Monócitos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
33X04XA5AT (Lactic Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE


  8 / 3213 MEDLINE  
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[PMID]:28376905
[Au] Autor:Bao Y; Tian M; Li P; Liu J; Ding C; Yu S
[Ad] Endereço:Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China.
[Ti] Título:Characterization of Brucella abortus mutant strain Δ22915, a potential vaccine candidate.
[So] Source:Vet Res;48(1):17, 2017 Apr 04.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Brucellosis, caused by Brucella spp., is an important zoonosis worldwide. Vaccination is an effective strategy for protection against Brucella infection in livestock in developing countries and in wildlife in developed countries. However, current vaccine strains including S19 and RB51 are pathogenic to humans and pregnant animals, limiting their use. In this study, we constructed the Brucella abortus (B. abortus) S2308 mutant strain Δ22915, in which the putative lytic transglycosylase gene BAB_RS22915 was deleted. The biological properties of mutant strain Δ22915 were characterized and protection of mice against virulent S2308 challenge was evaluated. The mutant strain Δ22915 showed reduced survival within RAW264.7 cells and survival in vivo in mice. In addition, the mutant strain Δ22915 failed to escape fusion with lysosomes within host cells, and caused no observable pathological damage. RNA-seq analysis indicated that four genes associated with amino acid/nucleotide transport and metabolism were significantly upregulated in mutant strain Δ22915. Furthermore, inoculation of ∆22915 at 10 colony forming units induced effective host immune responses and long-term protection of BALB/c mice. Therefore, mutant strain ∆22915 could be used as a novel vaccine candidate in the future to protect animals against B. abortus infection.
[Mh] Termos MeSH primário: Vacina contra Brucelose/imunologia
Brucella abortus/genética
Brucelose Bovina/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Aderência Bacteriana/imunologia
Vacina contra Brucelose/uso terapêutico
Brucella abortus/imunologia
Brucelose Bovina/imunologia
Brucelose Bovina/microbiologia
Bovinos
Feminino
Imunofluorescência/veterinária
Camundongos
Camundongos Endogâmicos BALB C
Células RAW 264.7
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Brucella Vaccine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-017-0422-9


  9 / 3213 MEDLINE  
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[PMID]:28334833
[Au] Autor:Kleinman CL; Sycz G; Bonomi HR; Rodríguez RM; Zorreguieta A; Sieira R
[Ad] Endereço:Lady Davis Institute, McGill University, Montreal, QC H3T 1E2, Canada.
[Ti] Título:ChIP-seq analysis of the LuxR-type regulator VjbR reveals novel insights into the Brucella virulence gene expression network.
[So] Source:Nucleic Acids Res;45(10):5757-5769, 2017 Jun 02.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:LuxR-type transcription factors control diverse physiological functions necessary for bacterial adaptation to environmental changes. In the intracellular pathogen Brucella, the LuxR homolog VjbR has been shown to regulate the expression of virulence factors acting at early stages of the intracellular infection and, directly or indirectly, hundreds of additional genes. However, the precise determination of VjbR direct targets has so far proved elusive. Here, we performed chromatin immunoprecipitation of VjbR followed by next-generation sequencing (ChIP-seq). We detected a large amount of VjbR-binding sites distributed across the Brucella genome and determined a markedly asymmetric binding consensus motif, an unusual feature among LuxR-type regulators. RNA-seq analysis performed under conditions mimicking the eukaryotic intracellular environment revealed that, among all loci associated to VjbR-binding, this regulator directly modulated the expression of only a subset of genes encoding functions consistent with an intracellular adaptation strategy for survival during the initial stages of the host cell infection. Other VjbR-binding events, however, showed to be dissociated from transcription and may require different environmental signals to produce a transcriptional output. Taken together, our results bring new insights into the extent and functionality of LuxR-type-related transcriptional networks.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Brucella abortus/genética
Brucella abortus/patogenicidade
Regulação Bacteriana da Expressão Gênica
Redes Reguladoras de Genes
Proteínas Repressoras/genética
Transativadores/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Sítios de Ligação
Brucella abortus/metabolismo
Imunoprecipitação da Cromatina
Sequenciamento de Nucleotídeos em Larga Escala
Motivos de Nucleotídeos
Ligação Proteica
Percepção de Quorum/genética
Proteínas Repressoras/metabolismo
Transativadores/metabolismo
Transcrição Genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Repressor Proteins); 0 (Trans-Activators); 115038-68-1 (LuxR autoinducer binding proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx165


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[PMID]:28284850
[Au] Autor:Hashemifar I; Yadegar A; Jazi FM; Amirmozafari N
[Ad] Endereço:Microbiology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
[Ti] Título:Molecular prevalence of putative virulence-associated genes in Brucella melitensis and Brucella abortus isolates from human and livestock specimens in Iran.
[So] Source:Microb Pathog;105:334-339, 2017 Apr.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Molecular prevalence of nine putative virulence factors in two more prevalent Brucella species in Iranian patients and livestock was investigated. During five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of Iran. All samples were cultured in blood culture media and subcultured into Brucella agar medium. Nine primer pairs were designed for detection of VirB2, VirB5, VceC, BtpA, BtpB, PrpA, BetB, BPE275 and BSPB virulence factors using PCR and sequence analysis. Totally, 68 Brucella isolates including 60 B. melitensis and 8 B. abortus were isolated from the human and animal specimens examined. Approximately, all B. melitensis and B. abortus strains were positive (100%) regarding btpA, btpB, virB5, vceC, bpe275, bspB, and virB2 genes except for prpA and betB that were detected in 86% and 97% of the strains, respectively. Significant relationships were found between the presence of prpA and human B. melitensis isolates (P = 0.04), and also between the presence of betB and human isolates of B. abortus (P = 0.03). In conclusion, our results revealed that Iranian Brucella strains, regardless of human or animal sources, are extremely virulent due to high prevalence of virulence attributes in almost all strains studied.
[Mh] Termos MeSH primário: Brucella abortus/genética
Brucella melitensis/genética
Brucelose/microbiologia
Brucelose/veterinária
Gado/microbiologia
Fatores de Virulência/genética
[Mh] Termos MeSH secundário: Doenças dos Animais/microbiologia
Animais
Brucella abortus/isolamento & purificação
Brucella abortus/patogenicidade
Brucella melitensis/isolamento & purificação
Brucella melitensis/patogenicidade
Brucelose/sangue
Brucelose/epidemiologia
DNA Bacteriano/genética
Feminino
Genes Bacterianos
Genótipo
Seres Humanos
Irã (Geográfico)/epidemiologia
Masculino
Prevalência
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Virulence Factors)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE



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