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[PMID]:29185955
[Au] Autor:Choi GM; Im WT
[Ad] Endereço:1​Department of Biotechnology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 456-749, Republic of Korea.
[Ti] Título:Paraburkholderia azotifigens sp. nov., a nitrogen-fixing bacterium isolated from paddy soil.
[So] Source:Int J Syst Evol Microbiol;68(1):310-316, 2018 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain, designated NF2-5-3 , was isolated from a paddy soil in Anseong city, Republic of Korea. This bacterium was characterized to determine its taxonomic position using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NF2-5-3 had a close relationship with, and was related most closely to, members of the genus Paraburkholderia, namely Paraburkholderia caribensis MWAP64 (98.8 % similarity), P. sabiae Br3407 (98.8 %), P. hospita LMG 20598 (98.5 %), P. terrae NBRC 100964 (98.3 %) and P. phymatum STM815 (98.1 %). Growth of strain NF2-5-3 occurred at 15-37 °C, at pH 6.0-8.0 and at NaCl concentrations of 0-2 % (w/v). The major respiratory quinone was ubiquinone 8 (Q-8) and the major fatty acids were C16 : 0, summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C17 : 0 cyclo and C16 : 0 3-OH. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, unidentified aminophospholipids, unidentified aminolipids and unidentified polar lipids. The G+C content of the genomic DNA was 64.2 mol%. DNA-DNA relatedness values between strain NF2-5-3 and its closest phylogenetic neighbours were much lower than 70 %. Strain NF2-5-3 could be differentiated phylogenetically and phenotypically from recognized species of the genus Paraburkholderia. The isolate therefore represents a novel species, for which the name Paraburkholderia azotifigens sp. nov. is proposed, with NF2-5-3 (=KACC 18968 =LMG 29961 ) as the type strain.
[Mh] Termos MeSH primário: Burkholderiaceae/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Burkholderiaceae/genética
Burkholderiaceae/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Fixação de Nitrogênio
Hibridização de Ácido Nucleico
Oryza
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002505


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[PMID]:28829016
[Au] Autor:Hahn MW; Karbon G; Koll U; Schmidt J; Lang E
[Ad] Endereço:1​Research Institute for Limnology, University of Innsbruck, Mondseestrasse 9, A-5310 Mondsee, Austria.
[Ti] Título:Polynucleobacter sphagniphilus sp. nov. a planktonic freshwater bacterium isolated from an acidic and humic freshwater habitat.
[So] Source:Int J Syst Evol Microbiol;67(9):3261-3267, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Strain MWH-Weng1-1T, isolated from an acidic freshwater habitat located in the Wenger Moor, Austria, was characterized by investigating its phenotypic, chemotaxonomic and genomic traits. Phylogenetic analyses based on 16S rRNA gene sequencing placed the strain in the cryptic species complex PnecC within the genus Polynucleobacter. The strain had a genome of 2.04 Mbp with a G+C content of 45.6 mol%. The major fatty acids of the strain were C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. In order to resolve the systematic position of the strain within the species complex PnecC, concatenated partial sequences of eight housekeeping genes were used for phylogenetic analyses. The obtained trees did not place strain MWH-Weng1-1T close to any of the six previously described species within this cryptic species complex. Pairwise whole genome average nucleotide identity comparisons with genome sequences of strains representing the six previously described species of the subcluster resulted throughout in values <78 %, which clearly suggested that strain MWH-Weng1-1T (DSM 24018T=CIP 111099T) represents a novel species. We propose the name Polynucleobacter sphagniphilus sp. nov. and strain MWH-Weng1-1T as the type strain for this new species.
[Mh] Termos MeSH primário: Burkholderiaceae/classificação
Água Doce/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Ácidos
Áustria
Técnicas de Tipagem Bacteriana
Composição de Bases
Burkholderiaceae/genética
Burkholderiaceae/isolamento & purificação
DNA Bacteriano/genética
Ecossistema
Ácidos Graxos/química
Genes Bacterianos
Plâncton/classificação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002096


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[PMID]:28684420
[Au] Autor:Motl N; Skiba MA; Kabil O; Smith JL; Banerjee R
[Ad] Endereço:From the Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0600 and.
[Ti] Título:Structural and biochemical analyses indicate that a bacterial persulfide dioxygenase-rhodanese fusion protein functions in sulfur assimilation.
[So] Source:J Biol Chem;292(34):14026-14038, 2017 Aug 25.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hydrogen sulfide (H S) is a signaling molecule that is toxic at elevated concentrations. In eukaryotes, it is cleared via a mitochondrial sulfide oxidation pathway, which comprises sulfide quinone oxidoreductase, persulfide dioxygenase (PDO), rhodanese, and sulfite oxidase and converts H S to thiosulfate and sulfate. Natural fusions between the non-heme iron containing PDO and rhodanese, a thiol sulfurtransferase, exist in some bacteria. However, little is known about the role of the PDO-rhodanese fusion (PRF) proteins in sulfur metabolism. Herein, we report the kinetic properties and the crystal structure of a PRF from the Gram-negative endophytic bacterium The crystal structures of wild-type PRF and a sulfurtransferase-inactivated C314S mutant with and without glutathione were determined at 1.8, 2.4, and 2.7 Å resolution, respectively. We found that the two active sites are distant and do not show evidence of direct communication. The PRF exhibited robust PDO activity and preferentially catalyzed sulfur transfer in the direction of thiosulfate to sulfite and glutathione persulfide; sulfur transfer in the reverse direction was detectable only under limited turnover conditions. Together with the kinetic data, our bioinformatics analysis reveals that PRF is poised to metabolize thiosulfate to sulfite in a sulfur assimilation pathway rather than in sulfide stress response as seen, for example, with the PRF or sulfide oxidation and disposal as observed with the homologous mammalian proteins.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Burkholderiaceae/enzimologia
Modelos Moleculares
Proteínas Mutantes Quiméricas/metabolismo
Quinona Redutases/metabolismo
Tiossulfato Sulfurtransferase/metabolismo
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Apoenzimas/química
Apoenzimas/genética
Apoenzimas/metabolismo
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Biocatálise
Domínio Catalítico
Biologia Computacional
Cristalografia por Raios X
Cisteína/química
Dissulfetos/metabolismo
Estabilidade Enzimática
Glutationa/análogos & derivados
Glutationa/química
Glutationa/metabolismo
Sulfeto de Hidrogênio/metabolismo
Proteínas Mutantes Quiméricas/química
Proteínas Mutantes Quiméricas/genética
Mutação
Fragmentos de Peptídeos/química
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Conformação Proteica
Quinona Redutases/química
Quinona Redutases/genética
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Tiossulfato Sulfurtransferase/química
Tiossulfato Sulfurtransferase/genética
Tiossulfatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoenzymes); 0 (Bacterial Proteins); 0 (Disulfides); 0 (Mutant Chimeric Proteins); 0 (Peptide Fragments); 0 (Recombinant Proteins); 0 (Thiosulfates); 0 (glutathione persulfide); EC 1.6.99.- (Quinone Reductases); EC 1.8.5.- (sulfide quinone reductase); EC 2.8.1.1 (Thiosulfate Sulfurtransferase); GAN16C9B8O (Glutathione); K848JZ4886 (Cysteine); YY9FVM7NSN (Hydrogen Sulfide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.790170


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[PMID]:28671521
[Au] Autor:Pheng S; Lee JJ; Eom MK; Lee KH; Kim SG
[Ad] Endereço:1​Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, 181 Ipsingil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea 2​University of Science and Technology (UST), 217 Gajeongro, Yuseong, Daejeon 34113, Republic of Kor
[Ti] Título:Paucibacter oligotrophus sp. nov., isolated from fresh water, and emended description of the genus Paucibacter.
[So] Source:Int J Syst Evol Microbiol;67(7):2231-2235, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, rod-shaped, non-spore forming, motile and strictly oxidative bacterium, strain CHU3T, was isolated from fresh water in the Daecheong Reservoir, South Korea. A comparison of the 16S rRNA gene sequence showed that the novel bacterium is closely related to Paucibacter toxinivorans 2C20T (=KCTC 42569T) with a sequence similarity value of 97.8 %, Pelomonas saccharophila DSM 654T (=KCTC 52256T) with 97.4 % similarity and Pelomonas aquatica CCUG 52575T (=KCTC 42961T) with 97.3 % similarity, respectively. The major fatty acids (>10 %) of the isolate were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. Ubiquinone-8 was detected as the respiratory quinone. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and an unidentified aminolipid. The DNA G+C content was 62.5 mol%. DNA-DNA hybridization experiments with PaucibactertoxinivoransKCTC 42569T (=2C20T), PelomonassaccharophilaKCTC 52256T (=DSM 654T) and PelomonasaquaticaKCTC 42961T (=CCUG 52575T) resulted in relatedness values of 20 % (reciprocal 11 %), 16 % (reciprocal 17 %) and 15 % (reciprocal 19 %), respectively. The phylogenetic analysis, DNA-DNA hybridization value, polar lipids, fatty acid composition and other physiological characteristics confirmed that strain CHU3T represents a novel species in the genus Paucibacter for which the name Paucibacter oligotrophus sp. nov. is proposed. The type strain is CHU3T (=KCTC 42519T=CICC 24092T). An emended description of the genus Paucibacter is also proposed on the basis of new data obtained in this study.
[Mh] Termos MeSH primário: Burkholderiaceae/classificação
Água Doce/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Burkholderiaceae/genética
Burkholderiaceae/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001931


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[PMID]:28370478
[Au] Autor:Kellici TF; Mavromoustakos T; Jendrossek D; Papageorgiou AC
[Ad] Endereço:Department of Chemistry, National and Kapodistrian University of Athens, Athens, 15784, Greece.
[Ti] Título:Crystal structure analysis, covalent docking, and molecular dynamics calculations reveal a conformational switch in PhaZ7 PHB depolymerase.
[So] Source:Proteins;85(7):1351-1361, 2017 Jul.
[Is] ISSN:1097-0134
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An open and a closed conformation of a surface loop in PhaZ7 extracellular poly(3-hydroxybutyrate) depolymerase were identified in two high-resolution crystal structures of a PhaZ7 Y105E mutant. Molecular dynamics (MD) simulations revealed high root mean square fluctuations (RMSF) of the 281-295 loop, in particular at residue Asp289 (RMSF 7.62 Å). Covalent docking between a 3-hydroxybutyric acid trimer and the catalytic residue Ser136 showed that the binding energy of the substrate is significantly more favorable in the open loop conformation compared to that in the closed loop conformation. MD simulations with the substrate covalently bound depicted 1 Å RMSF higher values for the residues 281-295 in comparison to the apo (substrate-free) form. In addition, the presence of the substrate in the active site enhanced the ability of the loop to adopt a closed form. Taken together, the analysis suggests that the flexible loop 281-295 of PhaZ7 depolymerase can act as a lid domain to control substrate access to the active site of the enzyme. Proteins 2017; 85:1351-1361. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Burkholderiaceae/química
Hidrolases de Éster Carboxílico/química
Hidroxibutiratos/química
Simulação de Dinâmica Molecular
Poliésteres/química
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Bacillus subtilis/genética
Bacillus subtilis/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Sítios de Ligação
Burkholderiaceae/enzimologia
Hidrolases de Éster Carboxílico/genética
Hidrolases de Éster Carboxílico/metabolismo
Domínio Catalítico
Clonagem Molecular
Cristalografia por Raios X
Expressão Gênica
Hidroxibutiratos/metabolismo
Cinética
Simulação de Acoplamento Molecular
Mutação
Poliésteres/metabolismo
Ligação Proteica
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Estrutura Terciária de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Hydroxybutyrates); 0 (Polyesters); 0 (Recombinant Proteins); 26063-00-3 (poly-beta-hydroxybutyrate); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.- (PHAZ7 protein, Paucimonas lemoignei)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1002/prot.25296


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[PMID]:28336132
[Au] Autor:Greninger AL; Streithorst J; Golden JA; Chiu CY; Miller S
[Ad] Endereço:Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
[Ti] Título:Complete genome sequence of sequential Pandoraea apista isolates from the same cystic fibrosis patient supports a model of chronic colonization with in vivo strain evolution over time.
[So] Source:Diagn Microbiol Infect Dis;87(1):1-6, 2017 Jan.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pandoraea apista in the family Burkholderiaceae is an emerging opportunistic pathogen in cystic fibrosis patients. Here, we describe a case from which 3 separate isolates of P. apista were recovered over a 1-year period. Using a combination of first-, second-, and third-generation sequencing technologies, we sequenced and de novo assembled the complete genomes of these 3 P. apista isolates. The genome of P. apista TF81F4 sequenced in this study was 5.58 Mb with a GC% of 62.3%, differed in sequence from other Pandoraea species by >20%, and included a number of previously undescribed loci. Three P. apista isolates cultured over a 12-month period were >99.999% identical by nucleotide, consistent with a model of chronic colonization by a single strain. Over time, the isolates accumulated point mutations, deletions, and insertions in a stepwise fashion, indicating in vivo strain evolution within the cystic fibrosis lung niche.
[Mh] Termos MeSH primário: Infecções por Burkholderia/microbiologia
Burkholderiaceae/isolamento & purificação
Fibrose Cística/complicações
DNA Bacteriano/genética
Evolução Molecular
Variação Genética
Genoma Bacteriano
[Mh] Termos MeSH secundário: Composição de Bases
Burkholderiaceae/classificação
Burkholderiaceae/genética
DNA Bacteriano/química
Genótipo
Seres Humanos
Masculino
Mutação
Análise de Sequência de DNA
Fatores de Tempo
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


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[PMID]:28173787
[Au] Autor:Martina PF; Martínez M; Frada G; Alvarez F; Leguizamón L; Prieto C; Barrias C; Bettiol M; Lagares A; Bosch A; Ferreras J; Von Specht M
[Ad] Endereço:Instituto de Biología Subtropical (IBS), CONICET-UNaM, Misiones, Argentina.
[Ti] Título:First time identification of Pandoraea sputorum from a patient with cystic fibrosis in Argentina: a case report.
[So] Source:BMC Pulm Med;17(1):33, 2017 Feb 07.
[Is] ISSN:1471-2466
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Pandoraea species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, Pandoraea sputorum has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to P. sputorum in a CF patient in Argentina. CASE PRESENTATION: Pandoraea sputorum was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of P. sputorum, patient's clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim-sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of P. sputorum. CONCLUSIONS: All combined data showed a chronic colonization with P. sputorum associated to a deterioration of lung function. We noted that the presence of P. sputorum can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of Pandoraea species.
[Mh] Termos MeSH primário: Burkholderiaceae/genética
Burkholderiaceae/isolamento & purificação
Fibrose Cística/microbiologia
[Mh] Termos MeSH secundário: Argentina
Criança
Seres Humanos
Masculino
RNA Ribossômico 16S/genética
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Escarro/microbiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1186/s12890-017-0373-y


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[PMID]:28110615
[Au] Autor:Kasai D; Imai S; Asano S; Tabata M; Iijima S; Kamimura N; Masai E; Fukuda M
[Ad] Endereço:a Department of Bioengineering , Nagaoka University of Technology , Nagaoka , Japan.
[Ti] Título:Identification of natural rubber degradation gene in Rhizobacter gummiphilus NS21.
[So] Source:Biosci Biotechnol Biochem;81(3):614-620, 2017 Mar.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-negative rubber-degrading bacterium, Rhizobacter gummiphilus NS21 grew and produced aldehyde metabolites on a deproteinized natural rubber (DPNR)-overlay agar medium forming a clearing zone. A transposon-insertion mutant, which had lost the ability to degrade DPNR, was isolated to identify the rubber degradation genes. Sequencing analysis indicated that the transposon was inserted into a putative oxygenase gene, latA. The deduced amino acid sequence of latA has 36% identity with that of roxA, which encodes a rubber oxygenase of Xanthomonas sp. strain 35Y. Phylogenetic analysis revealed that LatA constitutes a distinct group from RoxA. Heterologous expression in a Methylibium host and deletion analysis of latA indicated that the latA product is responsible for the depolymerization of DPNR. The quantitative reverse transcription-PCR analysis indicated that the transcription of latA is induced during the growth on DPNR. These results strongly suggest that latA is directly involved in the degradation of rubber in NS21.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Burkholderiaceae/genética
Oxigenases/genética
Borracha/metabolismo
[Mh] Termos MeSH secundário: Betaproteobacteria/genética
Biodegradação Ambiental
Burkholderiaceae/metabolismo
Regulação Bacteriana da Expressão Gênica
Genes Bacterianos
Oxigenases/metabolismo
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 9006-04-6 (Rubber); EC 1.13.- (Oxygenases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2016.1263147


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[PMID]:27902302
[Au] Autor:Hahn MW; Huymann LR; Koll U; Schmidt J; Lang E; Hoetzinger M
[Ad] Endereço:1​Research Institute for Limnology, University of Innsbruck, Mondseestrasse 9, Mondsee A-5310, Austria.
[Ti] Título:Polynucleobacter wuianus sp. nov., a free-living freshwater bacterium affiliated with the cryptic species complex PnecC.
[So] Source:Int J Syst Evol Microbiol;67(2):379-385, 2017 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Strain QLW-P1FAT50C-4T, isolated from a shallow, acidic freshwater pond located in the Austrian Alps at an altitude of 1300 m, was characterized by investigation of phenotypic, chemotaxonomic and genomic traits. As shown previously, phylogenetic analyses based on 16S rRNA gene sequences placed the strain in the cryptic species complex PnecC within the genus Polynucleobacter. The major fatty acids of the strain were C16 : 1ω7c and C18 : 1ω7c. The strain has a genome of 2.23 Mbp with a DNA G+C content of 44.9 mol%. The strain encodes a seemingly complete gene cluster for anoxygenic photosynthesis but lacks typical genes for CO2 assimilation. In order to resolve the phylogenetic position of the strain within the species complex PnecC, concatenated partial sequences of eight housekeeping genes were analysed. The phylogenetic reconstruction obtained did not place strain QLW-P1FAT50C-4T close to any of the five previously described species within subcluster PnecC. Pairwise average nucleotide identity (ANI) comparisons of whole-genome sequences suggested that strain QLW-P1FAT50C-4T (=DSM 24008T=CIP 111100T) represents a novel species, for which we propose the name Polynucleobacter wuianus sp. nov.
[Mh] Termos MeSH primário: Burkholderiaceae/classificação
Água Doce/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Áustria
Técnicas de Tipagem Bacteriana
Composição de Bases
Burkholderiaceae/genética
Burkholderiaceae/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001637


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[PMID]:27902193
[Au] Autor:Chen WM; Xie PB; Young CC; Sheu SY
[Ad] Endereço:1​Department of Seafood Science, Laboratory of Microbiology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
[Ti] Título:Formosimonas limnophila gen. nov., sp. nov., a new member of the family Burkholderiaceae isolated from a freshwater lake.
[So] Source:Int J Syst Evol Microbiol;67(1):17-24, 2017 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Strain AHQ-12T, isolated from a freshwater lake in Taiwan, was characterized using a polyphasic taxonomy approach. Cells of strain AHQ-12T were Gram-staining-negative, aerobic, non-motile, non-spore forming, straight rods and formed translucent white-coloured colonies. Optimal growth occurred at 20 °C, pH 6.0 and with 0 % NaCl. The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The major isoprenoid quinone was Q-8, and the DNA G+C content was 50.4 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and lipids. The major polyamine was cadaverine. 16S rRNA gene sequence analysis demonstrated that this isolate was unique, showing less than 91 % sequence similarity to its closest relatives, including members of the genera Ralstonia (89.7-90.8 %), Cupriavidus (88.8-90.3 %), Polynucleobacter (88.2-89.5 %), Burkholderia (86.6-90.3 %) and Pandoraea (89.2-90.1 %). Phylogenetic analyses demonstrated that strain AHQ-12T formed a distinct clade closely related to species of the family Burkholderiaceae. On the basis of the phylogenetic inference and phenotypic data, strain AHQ-12T should be classified as a novel species of a new genus in the family Burkholderiaceae, for which the name Formosimonas limnophila gen. nov., sp. nov. is proposed. The type strain is AHQ-12T (=BCRC 80690T=LMG 27847T=KCTC 32501T).
[Mh] Termos MeSH primário: Burkholderiaceae/classificação
Lagos/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Burkholderiaceae/genética
Burkholderiaceae/isolamento & purificação
Cadaverina/química
DNA Bacteriano/genética
Ácidos Graxos/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Taiwan
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8); L90BEN6OLL (Cadaverine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001561



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