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[PMID]:29211972
[Au] Autor:Punjee P; Siripornadulsil W; Siripornadulsil S
[Ad] Endereço:a Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
[Ti] Título:Reduction of cadmium uptake in rice endophytically colonized with the cadmium-tolerant bacterium Cupriavidus taiwanensis KKU2500-3.
[So] Source:Can J Microbiol;64(2):131-145, 2018 Feb.
[Is] ISSN:1480-3275
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:The effects of the cadmium (Cd)-tolerant bacterium Cupriavidus taiwanensis KKU2500-3 on the growth, yield, and Cd concentration in rice grains were investigated in the rice variety Phitsanulok 2 (PL2), which was cultivated in a hydroponic greenhouse. The numbers of Cd-tolerant bacteria isolated from the roots and shoots of plants under the RB (rice with bacteria) and RBC (rice with bacteria and Cd) treatments ranged from 2.60 to 9.03 and from 3.99 to 9.60 log cfu·g of PL2, respectively. This KKU2500-3 strain was successfully colonized in rice, indicating that it was not only nontoxic to the plants but also became distributed and reproduced throughout the plants. Scanning electron microscopy analysis revealed attachment of the bacterium to the root surface, whereas the internally colonized bacteria were located in the vascular tissue, cell wall, and intercellular space. Although the Cd contents found in PL2 were very high (189.10 and 79.49 mg·kg in the RC (rice with Cd) and RBC roots, respectively), the Cd accumulated inside the rice seeds at densities of only 3.10 and 1.31 mg·kg , respectively; thus, the bacteria reduced the Cd content to 57.74% of the control content. Therefore, the colonizing bacteria likely acted as an inhibitor of Cd translocation in PL2.
[Mh] Termos MeSH primário: Cádmio/metabolismo
Cupriavidus/fisiologia
Oryza/metabolismo
Oryza/microbiologia
[Mh] Termos MeSH secundário: Cádmio/análise
Cupriavidus/ultraestrutura
Microscopia Eletrônica de Varredura
Oryza/química
Oryza/crescimento & desenvolvimento
Raízes de Plantas/microbiologia
Raízes de Plantas/ultraestrutura
Sementes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
00BH33GNGH (Cadmium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1139/cjm-2017-0198


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[PMID]:28457658
[Au] Autor:Huong KH; Azuraini MJ; Aziz NA; Amirul AA
[Ad] Endereço:Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, 11700 Gelugor, Penang, Malaysia.
[Ti] Título:Pilot scale production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) biopolymers with high molecular weight and elastomeric properties.
[So] Source:J Biosci Bioeng;124(1):76-83, 2017 Jul.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [(P(3HB-co-4HB)] copolymer receives attention as next generation biomaterial in medical application. However, the exploitation of the copolymer is still constrained since such copolymer has not yet successfully been performed in industrial scale production. In this work, we intended to establish pilot production system of the copolymer retaining the copolymer quality which has recently discovered to have novel characteristic from lab scale fermentation. An increase of agitation speed has significantly improved the copolymer accumulation efficiency by minimizing the utilization of substrates towards cell growth components. This is evidenced by a drastic increase of PHA content from 28 wt% to 63 wt% and PHA concentration from 3.1 g/L to 6.5 g/L but accompanied by the reduction of residual biomass from 8.0 g/L to 3.8 g/L. Besides, fermentations at lower agitation and aeration have resulted in reduced molecular weight and mechanical strength of the copolymer, suggesting the role of sufficient oxygen supply efficiency in improving the properties of the resulting copolymers. The K a-based scale-up fermentation was performed successfully in maintaining the yield and the quality of the copolymers produced without a drastic fluctuation. This suggests that the scale-up based on the K a values supported the fermentation system of P(3HB-co-4HB) copolymer production in single-stage using mixed-substrate cultivation strategy.
[Mh] Termos MeSH primário: Elastômeros/química
Elastômeros/metabolismo
Hidroxibutiratos/química
Hidroxibutiratos/metabolismo
Poliésteres/química
Poliésteres/metabolismo
[Mh] Termos MeSH secundário: Biomassa
Cupriavidus/metabolismo
Fermentação
Peso Molecular
Projetos Piloto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Elastomers); 0 (Hydroxybutyrates); 0 (Polyesters); 117068-64-1 (poly(3-hydroxybutyrate-co-4-hydroxybutyrate))
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:28434228
[Au] Autor:Wu X; Wang W; Liu J; Pan D; Tu X; Lv P; Wang Y; Cao H; Wang Y; Hua R
[Ad] Endereço:College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui Province, Anhui Agricultural University , Hefei 230036, People's Republic of China.
[Ti] Título:Rapid Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by Cupriavidus gilardii T-1.
[So] Source:J Agric Food Chem;65(18):3711-3720, 2017 May 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phytotoxicity and environmental pollution of residual herbicides have caused much public concern during the past several decades. An indigenous bacterial strain capable of degrading 2,4-dichlorophenoxyacetic acid (2,4-D), designated T-1, was isolated from soybean field soil and identified as Cupriavidus gilardii. Strain T-1 degraded 2,4-D 3.39 times more rapidly than the model strain Cupriavidus necator JMP134. T-1 could also efficiently degrade 2-methyl-4-chlorophenoxyacetic acid (MCPA), MCPA isooctyl ester, and 2-(2,4-dichlorophenoxy)propionic acid (2,4-DP). Suitable conditions for 2,4-D degradation were pH 7.0-9.0, 37-42 °C, and 4.0 mL of inoculums. Degradation of 2,4-D was concentration-dependent. 2,4-D was degraded to 2,4-dichlorophenol (2,4-DCP) by cleavage of the ether bond and then to 3,5-dichlorocatechol (3,5-DCC) via hydroxylation, followed by ortho-cleavage to cis-2-dichlorodiene lactone (CDL). The metabolites 2,4-DCP or 3,5-DCC at 10 mg L were completely degraded within 16 h. Fast degradation of 2,4-D and its analogues highlights the potential for use of C. gilardii T-1 in bioremediation of phenoxyalkanoic acid herbicides.
[Mh] Termos MeSH primário: Ácido 2,4-Diclorofenoxiacético/metabolismo
Cupriavidus/metabolismo
Herbicidas/metabolismo
[Mh] Termos MeSH secundário: Ácido 2,4-Diclorofenoxiacético/química
Biodegradação Ambiental
Cupriavidus/química
Cupriavidus/genética
Cupriavidus/isolamento & purificação
Herbicidas/química
Estrutura Molecular
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00544


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[PMID]:28346361
[Au] Autor:Andrews M; Andrews ME
[Ad] Endereço:Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 84, Lincoln 7647, New Zealand. mitchell.andrews@lincoln.ac.nz.
[Ti] Título:Specificity in Legume-Rhizobia Symbioses.
[So] Source:Int J Mol Sci;18(4), 2017 Mar 26.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Most species in the Leguminosae (legume family) can fix atmospheric nitrogen (N2) via symbiotic bacteria (rhizobia) in root nodules. Here, the literature on legume-rhizobia symbioses in field soils was reviewed and genotypically characterised rhizobia related to the taxonomy of the legumes from which they were isolated. The Leguminosae was divided into three sub-families, the Caesalpinioideae, Mimosoideae and Papilionoideae. spp. were the exclusive rhizobial symbionts of species in the Caesalpinioideae, but data are limited. Generally, a range of rhizobia genera nodulated legume species across the two Mimosoideae tribes Ingeae and Mimoseae, but spp. show specificity towards in central and southern Brazil, / in central Mexico and in southern Uruguay. These specific symbioses are likely to be at least in part related to the relative occurrence of the potential symbionts in soils of the different regions. Generally, Papilionoideae species were promiscuous in relation to rhizobial symbionts, but specificity for rhizobial genus appears to hold at the tribe level for the Fabeae ( ), the genus level for ( ), ( ) and the New Zealand native spp. ( ) and species level for ( ), ( ) and ( ). Specificity for rhizobial species/symbiovar appears to hold for ( sv. ) ( sv. ), ( ), ( sv. ), ( sv. s) and ( sv. ). Lateral gene transfer of specific symbiosis genes within rhizobial genera is an important mechanism allowing legumes to form symbioses with rhizobia adapted to particular soils. Strain-specific legume rhizobia symbioses can develop in particular habitats.
[Mh] Termos MeSH primário: Fabaceae/microbiologia
Rhizobium/fisiologia
Simbiose
[Mh] Termos MeSH secundário: Proteínas de Bactérias/classificação
Proteínas de Bactérias/genética
Bradyrhizobium/classificação
Bradyrhizobium/genética
Bradyrhizobium/fisiologia
Cupriavidus/classificação
Cupriavidus/fisiologia
Fabaceae/metabolismo
Filogenia
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
RNA Ribossômico 16S/genética
RNA Ribossômico 16S/metabolismo
Rhizobium/classificação
Rhizobium/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170507
[Lr] Data última revisão:
170507
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE


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[PMID]:28334155
[Au] Autor:Dall'Agnol RF; Bournaud C; de Faria SM; Béna G; Moulin L; Hungria M
[Ad] Endereço:Soil Biotechnology Laboratory, Embrapa Soja, C.P. 231, 86001-970, Londrina, PR, Brazil.
[Ti] Título:Genetic diversity of symbiotic Paraburkholderia species isolated from nodules of Mimosa pudica (L.) and Phaseolus vulgaris (L.) grown in soils of the Brazilian Atlantic Forest (Mata Atlântica).
[So] Source:FEMS Microbiol Ecol;93(4), 2017 Apr 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Some species of the genus Paraburkholderia that are able to nodulate and fix nitrogen in symbiosis with legumes are called ß-rhizobia and represent a group of ecological and biotechnological importance. We used Mimosa pudica and Phaseolus vulgaris to trap 427 rhizobial isolates from rhizospheric soil of Mimoseae trees in the Brazilian Atlantic Forest. Eighty-four representative strains were selected according to the 16S rRNA haplotypes and taxonomically characterized using a concatenated 16S rRNA-recA phylogeny. Most strains were assembled in the genus Paraburkholderia, including Paraburkholderia sabiae and Pa. nodosa. Mesorhizobium (α-rhizobia) and Cupriavidus (ß-rhizobia) were also isolated, but in smaller proportions. Multilocus sequence analysis and BOX-PCR analyses indicated that six clusters of Paraburkholderia represent potential new species. In the phylogenetic analysis of the nodC gene, the majority of the strains were positioned in the same groups as in the 16S rRNA-recA tree, indicative of stability and vertical inheritance, but we also identified horizontal transfer of nodC in Pa. sabiae. All α- and ß-rhizobial species were trapped by both legumes, although preferences of the host plants for specific rhizobial species have been observed.
[Mh] Termos MeSH primário: Betaproteobacteria/genética
Florestas
Variação Genética
Mimosa/microbiologia
Phaseolus/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Brasil
Cupriavidus/classificação
RNA Ribossômico 16S/genética
Rhizobium/genética
Solo
Microbiologia do Solo
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (Soil)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix027


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[PMID]:28248683
[Au] Autor:Yahya R; Alyousef W; Omara A; Alamoudi S; Alshami A; Abdalhamid B
[Ad] Endereço:King Fahad Specialist Hospital, Dammam, Kingdom of Saudi Arabia. Raghda.Yahya@kfsh.med.sa.
[Ti] Título:First case of pneumonia caused by Cupriavidus pauculus in an infant in the Gulf Cooperation Council.
[So] Source:J Infect Dev Ctries;11(2):196-198, 2017 Feb 28.
[Is] ISSN:1972-2680
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:Cupriavidus pauculus is an emerging organism causing infections in immunocompromised and immunocompetent patients. We report a C.pauculus pneumonia case susceptible to cefepime in an infant with end-stage renal failure. To our knowledge, this is the first case report of C. pauculus causing respiratory infections in the Gulf Cooperation Council.
[Mh] Termos MeSH primário: Cupriavidus/isolamento & purificação
Infecções por Bactérias Gram-Negativas/diagnóstico
Infecções por Bactérias Gram-Negativas/patologia
Pneumonia Bacteriana/diagnóstico
Pneumonia Bacteriana/patologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Cefalosporinas/farmacologia
Cupriavidus/efeitos dos fármacos
Infecções por Bactérias Gram-Negativas/microbiologia
Seres Humanos
Lactente
Falência Renal Crônica/complicações
Masculino
Testes de Sensibilidade Microbiana
Pneumonia Bacteriana/microbiologia
Arábia Saudita
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephalosporins); 807PW4VQE3 (cefepime)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE
[do] DOI:10.3855/jidc.8349


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[PMID]:28238184
[Au] Autor:Teng Y; Wang X; Zhu Y; Chen W; Christie P; Li Z; Luo Y
[Ad] Endereço:Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. yteng@issas.ac.cn.
[Ti] Título:Biodegradation of pentachloronitrobenzene by Cupriavidus sp. YNS-85 and its potential for remediation of contaminated soils.
[So] Source:Environ Sci Pollut Res Int;24(10):9538-9547, 2017 Apr.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Pentachloronitrobenzene (PCNB) is a toxic chlorinated nitroaromatic compound. However, only a few bacteria have been reported to be able to utilize PCNB. In the present study, one pentachloronitrobenzene (PCNB)-degrading bacterium, Cupriavidus sp. YNS-85, was isolated from a contaminated Panax notoginseng plantation. The strain co-metabolized 200 mg L PCNB in aqueous solution with a removal rate of 73.8% after 5 days. The bacterium also degraded PCNB effectively under acid conditions (pH 4-6) and showed resistance to toxic trace elements (arsenic, copper, and cadmium). Its ability to utilize proposed PCNB intermediates as sole carbon sources was also confirmed. The soil microcosm experiment further demonstrated that bacterial bioaugmentation enhanced the removal of PCNB (37.8%) from soil and the accumulation of pentachloroaniline (89.3%) after 30 days. Soil enzyme activity and microbial community functional diversity were positively influenced after bioremediation. These findings indicate that Cupriavidus sp. YNS-85 may be a suitable inoculant for in situ bioremediation of PCNB-polluted sites, especially those with acid soils co-contaminated with heavy metal(loid)s.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Cupriavidus/metabolismo
[Mh] Termos MeSH secundário: Nitrobenzenos/metabolismo
Solo
Microbiologia do Solo
Poluentes do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrobenzenes); 0 (Soil); 0 (Soil Pollutants); Q37G40S4S8 (quintozene)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-017-8640-2


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[PMID]:28154986
[Au] Autor:Wu ZG; Wang F; Ning LQ; Stedtfeld RD; Yang ZZ; Cao JG; Sheng HJ; Jiang X
[Ad] Endereço:College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology, Tianjin, 300457, People's Republic of China.
[Ti] Título:Biodegradation of 5-chloro-2-picolinic acid by novel identified co-metabolizing degrader Achromobacter sp. f1.
[So] Source:Biodegradation;28(2-3):139-144, 2017 Jun.
[Is] ISSN:1572-9729
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Several bacteria have been isolated to degrade 4-chloronitrobenzene. Degradation of 4-chloronitrobenzene by Cupriavidus sp. D4 produces 5-chloro-2-picolinic acid as a dead-end by-product, a potential pollutant. To date, no bacterium that degrades 5-chloro-2-picolinic acid has been reported. Strain f1, isolated from a soil polluted by 4-chloronitrobenzene, was able to co-metabolize 5-chloro-2-picolinic acid in the presence of ethanol or other appropriate carbon sources. The strain was identified as Achromobacter sp. based on its physiological, biochemical characteristics, and 16S rRNA gene sequence analysis. The organism completely degraded 50, 100 and 200 mg L of 5-chloro-2-picolinic acid within 48, 60, and 72 h, respectively. During the degradation of 5-chloro-2-picolinic acid, Cl was released. The initial metabolic product of 5-chloro-2-picolinic acid was identified as 6-hydroxy-5-chloro-2-picolinic acid by LC-MS and NMR. Using a mixed culture of Achromobacter sp. f1 and Cupriavidus sp. D4 for degradation of 4-chloronitrobenzen, 5-chloro-2-picolinic acid did not accumulate. Results infer that Achromobacter sp. f1 can be used for complete biodegradation of 4-chloronitrobenzene in remedial applications.
[Mh] Termos MeSH primário: Achromobacter/metabolismo
Ácidos Picolínicos/metabolismo
[Mh] Termos MeSH secundário: Achromobacter/isolamento & purificação
Biodegradação Ambiental
Cromatografia Líquida
Técnicas de Cocultura
Cupriavidus/metabolismo
Hidroxilação
Cinética
Espectrometria de Massas
Metaboloma
Nitrobenzenos/metabolismo
Espectroscopia de Prótons por Ressonância Magnética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-chloro-2-picolinic acid); 0 (Nitrobenzenes); 0 (Picolinic Acids); CVL66U249D (4-chloronitrobenzene); QZV2W997JQ (picolinic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1007/s10532-017-9783-z


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[PMID]:28119513
[Au] Autor:Xu H; Denny TP
[Ad] Endereço:College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, P.R. China.
[Ti] Título:Native and Foreign Proteins Secreted by the Type II System and an Alternative Mechanism.
[So] Source:J Microbiol Biotechnol;27(4):791-807, 2017 Apr 28.
[Is] ISSN:1738-8872
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:The type II secretion system (T2SS), which transports selected periplasmic proteins across the outer membrane, has rarely been studied in nonpathogens or in organisms classified as Betaproteobacteria. Therefore, we studied ( ), a facultative chemilithoautotroph. Gel analysis of extracellular proteins revealed no remarkable differences between the wild type and the T2SS mutants. However, enzyme assays revealed that native extracellular alkaline phosphatase is a T2SS substrate, because activity was 10-fold greater for the wild type than a T2SS mutant. In engineered to produce three ( ) exoenzymes, at least 95% of their total activities were extracellular, but unexpectedly high percentages of these exoenzymes remained extracellular in T2SS mutants cultured in rich broth. These conditions appear to permit an alternative secretion process, because neither cell lysis nor periplasmic leakage was observed when produced a exoenzyme, and wild-type cultured in minimal medium secreted 98% of polygalacturonase, but 92% of this exoenzyme remained intracellular in T2SS mutants. We concluded that has a functional T2SS despite lacking any abundant native T2SS substrates. The efficient secretion of three foreign exoenzymes by is remarkable, but so too is the indication of an alternative secretion process in rich culture conditions. When not transiting the T2SS, we suggest that exoenzymes are probably selectively packaged into outer membrane vesicles. Phylogenetic analysis of T2SS proteins supports the existence of at least three T2SS subfamilies, and we propose that , as a representative of the Betaproteobacteria, could become a new useful model system for studying T2SS substrate specificity.
[Mh] Termos MeSH primário: Proteínas de Bactérias/secreção
Cupriavidus/enzimologia
Cupriavidus/metabolismo
Sistemas de Secreção Tipo II/metabolismo
Sistemas de Secreção Tipo II/fisiologia
[Mh] Termos MeSH secundário: Fosfatase Alcalina/genética
Fosfatase Alcalina/secreção
Sequência de Aminoácidos
Proteínas de Bactérias/genética
Transporte Biológico
Hidrolases de Éster Carboxílico/genética
Hidrolases de Éster Carboxílico/secreção
Celulase/genética
Celulase/secreção
Cupriavidus/genética
DNA Bacteriano
Ensaios Enzimáticos
Escherichia coli/genética
Regulação Bacteriana da Expressão Gênica
Genes Bacterianos
Família Multigênica/genética
Mutação
Pectobacterium carotovorum/enzimologia
Filogenia
Poligalacturonase/genética
Poligalacturonase/secreção
Domínios Proteicos
Estrutura Secundária de Proteína
Sistemas de Translocação de Proteínas/classificação
Sistemas de Translocação de Proteínas/genética
Sistemas de Translocação de Proteínas/metabolismo
Sistemas de Translocação de Proteínas/fisiologia
Ralstonia solanacearum/enzimologia
Alinhamento de Sequência
Sistemas de Secreção Tipo II/classificação
Sistemas de Secreção Tipo II/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Protein Translocation Systems); 0 (Type II Secretion Systems); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.11 (pectinesterase); EC 3.1.3.1 (Alkaline Phosphatase); EC 3.2.1.15 (Polygalacturonase); EC 3.2.1.4 (Cellulase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.4014/jmb.1611.11002


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[PMID]:28110659
[Au] Autor:Syafiq IM; Huong KH; Shantini K; Vigneswari S; Aziz NA; Amirul AA; Bhubalan K
[Ad] Endereço:School of Marine and Environmental Sciences, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia; Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM MOSTI, Malaysia.
[Ti] Título:Synthesis of high 4-hydroxybutyrate copolymer by Cupriavidus sp. transformants using one-stage cultivation and mixed precursor substrates strategy.
[So] Source:Enzyme Microb Technol;98:1-8, 2017 Mar.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer is noted for its high biocompatibility, which makes it an excellent candidate for biopharmaceutical applications. The wild-type Cupriavidus sp. USMAA1020 strain is able to synthesize P(3HB-co-4HB) copolymers with different 4HB monomer compositions (up to 70mol%) in shaken flask cultures. Combinations of 4HB carbon precursors consisting of 1,6-hexanediol and γ-butyrolactone were applied for the production of P(3HB-co-4HB) with different 4HB molar fraction. A sharp increase in 4HB monomer composition was attained by introducing additional copies of PHA synthase gene (phaC), responsible for P(3HB-co-4HB) polymerization. The phaC of Cupriavidus sp. USMAA1020 and Cupriavidus sp. USMAA2-4 were cloned and heterologously introduced into host, wild-type Cupriavidus sp. USMAA1020. The gene dosage treatment resulted in the accumulation of 93mol% 4HB by the transformant strains when grown in similar conditions as the wild-type USMAA1020. The PHA synthase activities for both transformants were almost two-fold higher than the wild-type. The ability of the transformants to produce copolymers with high 4HB monomer composition was also tested in large scale production system using 5L and 30L bioreactors with a constant oxygen mass transfer rate. The 4HB monomer composition could be maintained at a range of 83-89mol%. The mechanical and thermal properties of copolymers improved with increasing 4HB monomer composition. The copolymers produced could be tailored for specific biopharmaceutical applications based on their properties.
[Mh] Termos MeSH primário: Cupriavidus/metabolismo
Hidroxibutiratos/metabolismo
Poliésteres/metabolismo
[Mh] Termos MeSH secundário: Aciltransferases/genética
Aciltransferases/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Reatores Biológicos/microbiologia
Biotecnologia
Cupriavidus/genética
Dosagem de Genes
Genes Bacterianos
Hidroxibutiratos/química
Microbiologia Industrial
Poliésteres/química
Transformação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Hydroxybutyrates); 0 (Polyesters); 117068-64-1 (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)); EC 2.3.- (Acyltransferases); EC 2.3.1.- (poly(3-hydroxyalkanoic acid) synthase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE



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